Metrohm 930 User Manual

930 Compact IC Flex

930 Compact IC Flex SeS

Manual

8.930.8007EN

Metrohm AG CH-9100 Herisau Switzerland

Phone [phone] Fax [phone] [e-mail] www.metrohm.com

930 Compact IC Flex

930 Compact IC Flex SeS

2.930.1400

Manual

8.930.8007EN

11.2013 zst

Teachware Metrohm AG CH-9100 Herisau

[e-mail]

This documentation is protected by copyright. All rights reserved.

Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above.

Documentation in additional languages can be found on

http://documents.metrohm.com.

Table of contents

Table of contents

1 Introduction		1
1.1	Instrument description .........................................................	1
1.2	Intended use .........................................................................	3
1.3	Safety instructions ................................................................	3
1.3.1	General notes on safety ...........................................................	3
1.3.2	Electrical safety ........................................................................	3
1.3.3	Tubing and capillary connections .............................................	4
1.3.4	Flammable solvents and chemicals ...........................................	5
1.3.5	Recycling and disposal .............................................................	5
1.4	Symbols and conventions ....................................................	5

2 Overview of the instrument		7
2.1	Front ......................................................................................	7
2.2	Rear ........................................................................................	8
2.3	Feed-throughs for capillaries and cables ..........................	10

3 Installation		13
3.1	Setting up the instrument ..................................................	13
3.1.1	Packaging ..............................................................................	13
3.1.2	Checks ..................................................................................	13
3.1.3	Location ................................................................................	13
3.2	Capillary connections in the IC system .............................	13
3.3	Removing transport locking screws .................................	16
3.4	Connecting the drainage tubing and leak sensor ............	17
3.4.1	Installing the drainage tubing ................................................	17
3.4.2	Connecting the leak sensor ....................................................	19
3.5	Connecting the eluent bottle .............................................	19
3.6	Installing the high-pressure pump ....................................	23
3.7	Installing an inline filter .....................................................	24
3.8	Installing the pulsation absorber ......................................	25
3.9	Injection valve .....................................................................	26
3.10	Suppressor ..........................................................................	28
3.10.1	Inserting the rotors ................................................................	28
3.10.2	Connecting the suppressor ....................................................	31
3.11	Metrohm CO2 Suppressor (MCS) .......................................	36
3.11.1 General information on MCS .................................................		36
3.11.2	Connecting the MCS .............................................................	37

930 Compact IC Flex SeS (2.930.1400)

III

Table of contents
	3.11.3	Installing adsorption cartridges ..............................................	38
	3.12	Installing the detector ........................................................	40
	3.13	Connecting the instrument to a computer .......................	40
	3.14	Connecting the instrument to the power supply .............	41
	3.15	Initial start-up .....................................................................	42
	3.16	Connecting and rinsing the guard column .......................	45
	3.17	Connecting the separation column ...................................	47
	3.18	Conditioning ........................................................................	50
4	Operation		52
5	Operation and maintenance		53
	5.1	IC system .............................................................................	53
	5.1.1	Operation ..............................................................................	53
	5.1.2	Care ......................................................................................	53
	5.1.3	Maintenance by Metrohm Service ..........................................	53
	5.1.4	Shutting down and starting back up ......................................	54
	5.2	Capillary connections .........................................................	55
	5.3	Servicing the door ..............................................................	55
	5.4	Handling the eluent ............................................................	55
	5.4.1	Manufacturing eluent ............................................................	56
	5.4.2	Changing the eluent ..............................................................	56
	5.5	Notes on operating the high-pressure pump ...................	57
	5.6	Servicing the high-pressure pump ....................................	58
	5.7	Servicing the inline filter ....................................................	71
	5.8	Servicing the pulsation absorber ......................................	74
	5.9	Injection valve .....................................................................	74
	5.10	Suppressor ..........................................................................	74
	5.10.1 Notes for operating the suppressor ........................................		74
	5.10.2 Taking care of the suppressor housing ...................................		75
	5.10.3	Servicing the suppressor ........................................................	75
	5.11	Servicing the Metrohm CO2 Suppressor (MCS) ................	82
	5.11.1 Replacing the CO2 adsorption cartridge .................................		82
	5.11.2 Regenerating the H2O adsorption cartridge ............................		82
	5.12	Servicing the detector ........................................................	83
	5.13	Rinsing the sample path ....................................................	83
	5.14	Separation column .............................................................	84
	5.14.1	Separating efficiency ..............................................................	84
	5.14.2 Protecting the separation column ..........................................		85
	5.14.3 Storing the separation column ...............................................		85
	5.14.4 Regenerating the separation column ......................................		85

IV	930 Compact IC Flex SeS (2.930.1400)

			Table of contents
	5.15	Quality Management and qualification with Metrohm . . 86
6	Troubleshooting		87
	6.1	.............................................................................................	87
7	Technical specifications		91
	7.1	Reference conditions ..........................................................	91
	7.2	Instrument ...........................................................................	91
	7.3	Ambient conditions ............................................................	91
	7.4	Housing ...............................................................................	92
	7.5	Leak sensor .........................................................................	92
	7.6	High-pressure pump ...........................................................	92
	7.7	Injection valve .....................................................................	93
	7.8	Suppressor ..........................................................................	93
	7.9	Metrohm CO2 Suppressor (MCS) .......................................	93
	7.10	Detector ...............................................................................	94
	7.11	Power connection ...............................................................	94
	7.12	Interfaces .............................................................................	94
	7.13	Safety specifications ...........................................................	95
	7.14	Electromagnetic compatibility (EMC) ................................	95
8	Warranty (guarantee)		96
9	Accessories		98
	Index		100

930 Compact IC Flex SeS (2.930.1400)

V

Table of figures

Table of figures
Figure 1	Front .................................................................................................	7
Figure 2	Rear ..................................................................................................	8
Figure 3	Feed-throughs on the door .............................................................	10
Figure 4	Openings for capillaries and cables ..................................................	11
Figure 5	Ducts for capillaries .........................................................................	12
Figure 6	Removing the transport locking screws ............................................	16
Figure 7	Installing the eluent bottle cap ........................................................	20
Figure 8	Installing tubing weighting and aspiration filter ...............................	21
Figure 9	High-pressure pump with purge valve ..............................................	24
Figure 10	Inline filter .......................................................................................	25
Figure 11	Pulsation absorber ...........................................................................	25
Figure 12	Exchanging the sample loop ............................................................	27
Figure 13	Suppressor – connection capillaries .................................................	31
Figure 14	Connecting the MCS .......................................................................	37
Figure 15	High-pressure pump – parts ............................................................	58
Figure 16	High-pressure pump – cross-section ................................................	65
Figure 17	Tool for piston seal (6.2617.010) .....................................................	66
Figure 18	Removing the piston cartridge from the pump head ........................	66
Figure 19	Inserting the piston seal into the tool ...............................................	67
Figure 20	Components of the piston cartridge ................................................	69
Figure 21	Inline filter – removing the filter .......................................................	72
Figure 22	Parts of the suppressor ....................................................................	75

VI	930 Compact IC Flex SeS (2.930.1400)

1 Introduction

1 Introduction

1.1Instrument description

The 930 Compact IC Flex is a professional ion chromatograph. It is distinguished by:

Its intelligence: All of the functions are monitored, optimized and documented in an FDA-compatible manner. Intelligent components, such as iColumns, save important data onto a chip.

Its compact design: It has a small footprint.

Its transparency: All components are easily accessible and located for simple visibility and can be monitored during operation through a large window.

Its safety: The wet end and electronics are physically separated, thereby preventing fluids from coming into contact with the electronics to a large extent. A leak sensor is integrated into the wet end.

Its environmental compatibility.

Its low noise emissions.

The intelligent MagIC Net™ software

The 930 Compact IC Flex is operated solely using the MagIC Net software. A USB cable is used to connect the instrument to a computer with

MagIC Net installed. The intelligent software detects the instrument automatically and checks its functionality. The software controls and monitors the instrument, evaluates the measured data and manages it in a database.

The 930 Compact IC Flex SeS consists of the following modules:

Leak sensor

The leak sensor detects leaking liquid that collects in the instrument's base tray. Liquid that leaks in the instrument is routed to the base tray using drainage tubing and detected there.

High-pressure pump

The intelligent and low-pulsation high-pressure pump pumps the eluent through the system. It is equipped with a chip where its technical specifications and "life history" (operating hours, service data, etc.) are saved.

Inline filter

Inline filters protect the separation column reliably from potential contamination from the eluent. The small filter pads with 2 µm pore size can be replaced quickly and easily. They remove particles from the solutions, such as bacteria and algae.

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1.1 Instrument description

Pulsation absorber

The pulsation absorber protects the separation column from damage caused by pressure fluctuations when switching the injection valve, and reduces interfering pulsations during highly sensitive measurements.

Injection valve

The injection valve connects the eluent path to the sample path. By a quick and precise switching of the valve a quantity of sample solution defined by the size of the sample loop is injected and flushed to the separation column with the eluent.

Suppressor

The suppressor consists of a suppressor drive, a rotor and, where applicable, an adapter. The suppressor drive gives you the flexibility to use different rotors according to the principle "one drive – many rotors". With appropriate adapters, the rotor for the sample preparation module (SPM Rotor) or suppressor rotors with different capacities and construction can be easily exchanged. The rotors are not included in the instrument's scope of delivery. A rotor suitable for the application must be ordered separately.

Metrohm CO2 Suppressor (MCS)

The Metrohm CO2 Suppressor (MCS) removes the CO2 from the eluent flow. This lowers the background conductivity, improves detection sensitivity and minimizes the injection peak and carbonate peak.

Detector

Metrohm offers a series of different detectors for various analysis tasks. A suitable detector type must be ordered as a separate accessory.

Separation column

The intelligent separation column separates different components according to their interactions with the column. Metrohm separation columns are equipped with a chip where their technical specifications and history (start-up, operating hours, etc) are stored.

2	930 Compact IC Flex SeS (2.930.1400)

1 Introduction

1.2Intended use

The 930 Compact IC Flex SeS is used for the determination of anions or polar substances with sequential suppression using ion chromatography.

Sequential suppression consists of:

Chemical suppression with a Metrohm Suppressor Module (MSM) and subsequent

CO2 suppression with the Metrohm CO2 Suppressor (MCS).

Background conductivity is reduced to a minimum with sequential suppression.

The instrument can also be used as needed for the determination of cations or anions without chemical suppression.

The present instrument is suitable for processing chemicals and flammable samples. Usage of the 930 Compact IC Flex therefore requires the user to have basic knowledge and experience in handling toxic and caustic substances. Knowledge with respect to the application of the fire prevention measures prescribed for laboratories is also mandatory.

1.3Safety instructions

1.3.1General notes on safety

WARNING

This instrument may only be operated in accordance with the specifications in this documentation.

This instrument has left the factory in a flawless state in terms of technical safety. To maintain this state and ensure non-hazardous operation of the instrument, the following instructions must be observed carefully.

1.3.2Electrical safety

The electrical safety when working with the instrument is ensured as part of the international standard IEC 61010.

WARNING

Only personnel qualified by Metrohm are authorized to carry out service work on electronic components.

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1.3 Safety instructions

WARNING

Never open the housing of the instrument. The instrument could be damaged by this. There is also a risk of serious injury if live components are touched.

There are no parts inside the housing which can be serviced or replaced by the user.

Mains voltage

WARNING

An incorrect mains voltage can damage the instrument.

Only operate this instrument with a mains voltage specified for it (see rear panel of the instrument).

Protection against electrostatic charges

WARNING

Electronic components are sensitive to electrostatic charges and can be destroyed by discharges.

Do not fail to pull the mains cable out of the mains connection socket before you set up or disconnect electrical plug connections at the rear of the instrument.

1.3.3Tubing and capillary connections

CAUTION

Leaks in tubing and capillary connections are a safety risk. Tighten all connections well by hand. Avoid applying excessive force to tubing connections. Damaged tubing ends lead to leakage. Appropriate tools can be used to loosen connections.

Check the connections regularly for leakage. If the instrument is used mainly in unattended operation, then weekly inspections are mandatory.

4	930 Compact IC Flex SeS (2.930.1400)

1 Introduction

1.3.4Flammable solvents and chemicals

WARNING

All relevant safety measures are to be observed when working with flammable solvents and chemicals.

Set up the instrument in a well-ventilated location (e.g. fume cupboard).

Keep all sources of flame far from the workplace.

Clean up spilled liquids and solids immediately.

Follow the safety instructions of the chemical manufacturer.

1.3.5Recycling and disposal

This product is covered by European Directive 2002/96/EC, WEEE – Waste from Electrical and Electronic Equipment.

The correct disposal of your old equipment will help to prevent negative effects on the environment and public health.

More details about the disposal of your old equipment can be obtained from your local authorities, from waste disposal companies or from your local dealer.

1.4Symbols and conventions

The following symbols and formatting may appear in this documentation:

	Cross-reference to figure legend
	The first number refers to the figure number, the sec-
	ond to the instrument part in the figure.
	Instruction step
	Carry out these steps in the sequence shown.
Method	Dialog text, parameter in the software
File New	Menu or menu item
[Next]	Button or key
	WARNING
	This symbol draws attention to a possible life-threat-
	ening hazard or risk of injury.

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1.4 Symbols and conventions

WARNING

This symbol draws attention to a possible hazard due to electrical current.

WARNING

This symbol draws attention to a possible hazard due to heat or hot instrument parts.

WARNING

This symbol draws attention to a possible biological hazard.

CAUTION

This symbol draws attention to possible damage to instruments or instrument parts.

NOTE

This symbol highlights additional information and tips.

6	930 Compact IC Flex SeS (2.930.1400)

2 Overview of the instrument

2 Overview of the instrument

2.1Front

Figure 1 Front

1	Bottle holder		2	Column holder
	Offers space for the eluent bottle and addi-			For hanging the separation column (iCol-
	tional accessories.			umn). With column recognition.
3	Column chamber		4	Suppressor
5	Inline filter		6	Injection valve
7	Pulsation absorber		8	Base tray
				With leak sensor.

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2.2 Rear

9Purge valve

For deaerating the high-pressure pump. With rotary knob in the center and pressure sensor.

11 Metrohm CO2 Suppressor (MCS)

10High-pressure pump

Pumps the eluent through the IC system.

12Detector chamber

Offers space for an embedded detector and additional accessories.

2.2Rear

	Figure 2	Rear
1	Bottle holder			2	Drainage tubing connection
					For connecting the drainage tubing, which
					guides escaped fluids away from the bottle
					holder.

8	930 Compact IC Flex SeS (2.930.1400)

				2 Overview of the instrument
3	Knurled screws		4	Back panel
	For fastening the removable back panel.			Removable. Enables access to the detector
				chamber.

5Drainage tubing connection

For connecting the drainage tubing, which guides escaped fluids away from the detector chamber.

6Exhaust opening

Labeled Exhaust. For extracting the air from the vacuum chamber.

7	Auxiliary connection socket		8	Service connection socket
	For connecting an 891 Professional Analog			For Metrohm service only.
	Out (2.891.0010).

9Transport locking screws

For securing the high-pressure pump when transporting the instrument.

10Drainage tubing connections

For connecting two drainage tubings that guide the escaped fluid to the leak sensor and from there to the waste container.

11	Base tray		12	Type plate
	With leak sensor.
13	Serial number		14	Leak sensor connection socket
				Labeled Leak Sensor. For connecting the
				leak sensor connection cable.

15Detector connection socket

Labeled Detector. For connecting Metrohm detectors.

17USB connection sockets

Labeled USB 1 and USB 2. For connecting USB devices.

19Power socket

Power socket for connecting the power cable and power switch for switching the instrument on and off.

16MSB connection sockets

Labeled MSB 1 and MSB 2. For connecting MSB devices.

18PC connection socket

For connecting the instrument to the computer with the USB cable (6.2151.020).

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9

2.3 Feed-throughs for capillaries and cables

2.3Feed-throughs for capillaries and cables

Multiple openings are available for leading capillaries into the instrument and for leading capillaries and cables out of the instrument:

Openings on the door

Openings on the back panel

Ducts between the instrument and the base tray as well as between the instrument and the bottle holder (see Figure 5, page 12)

Openings on the door

Figure 3 Feed-throughs on the door

1	Luer connector		2	Opening for capillaries
	For connecting a capillary from inside and			For up to 3 capillaries.
	for inserting a syringe (6.2816.020) from
	outside. For manual sample injection.

An opening for up to 3 capillaries is located on the door of the instrument.

The two Luer connections above are not actually openings; the capillaries are fastened to the Luer connection from within using PEEK pressure screws. You can use a syringe to inject or draw out liquid from the outside.

10	930 Compact IC Flex SeS (2.930.1400)

2 Overview of the instrument

Openings on the back panel

Figure 4 Openings for capillaries and cables

1	Openings for capillaries		2	Openings for cables

The removable back panel is outfitted with openings through which capillaries and cables can be lead out of the detector chamber.

Ducts for capillaries

There are ducts for capillaries between the instrument and base tray as well as between the instrument and the bottle holder. The capillaries can be fed to the front of the instrument from both sides of the instrument and from the front of the instrument to the back of the instrument.

930 Compact IC Flex SeS (2.930.1400)

11

2.3 Feed-throughs for capillaries and cables

Figure 5 Ducts for capillaries

12	930 Compact IC Flex SeS (2.930.1400)

3 Installation

3 Installation

3.1Setting up the instrument

3.1.1Packaging

The instrument is supplied in highly protective special packaging together with the separately packed accessories. Keep this packaging, as only this ensures safe transportation of the instrument.

3.1.2Checks

Immediately after receipt, check whether the shipment has arrived complete and without damage by comparing it with the delivery note.

3.1.3Location

The instrument has been developed for operation indoors and may not be used in explosive environments.

Place the instrument in a location of the laboratory which is suitable for operation, free of vibrations, protected from corrosive atmosphere, and contamination by chemicals.

The instrument should be protected against excessive temperature fluctuations and direct sunlight.

3.2Capillary connections in the IC system

Generally speaking, capillary connections between two components of an IC system are made up of one connection capillary and two pressure screws used to connect the capillary to the respective components.

Pressure screws

Three types of pressure screws are used in the IC system:

Number	Designation	Use
6.2744.010 / 6.2744.014	Pressure screw	On the injection valve
6.2744.070	Pressure screw, short	High-pressure pump, purge
		valve, inline filter, pulsation
		absorber, separation columns
6.2744.090	Pressure screw, long	MCS, sample degasser, 12-
		port valve

Pressure screws are tightened and loosened by hand. A tool is not needed.

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13

3.2 Capillary connections in the IC system
	Also see: PEEK pressure screws video on the Internet http://ic-
	help.metrohm.com/maintenance.php?chapter=1_2.
	Connection capillaries
	PEEK capillaries and PTFE capillaries are used in the IC system.
PEEK capillaries (poly-	PEEK capillaries are temperature-resistant up to 100 °C, stable under pres-
etheretherketone)	sure up to 400 bar (depending on the inner diameter), flexible, chemically
	inert and have an extremely smooth surface. They can be readily cut down
	to the desired length with the capillary cutter (6.2621.080).
	Use:
	PEEK capillaries with an inner diameter of 0.25 mm (6.1831.010) for
	the entire high-pressure section.
	PEEK capillaries with an inner diameter of 0.75 mm (6.1831.030) for
	sample processing in the ultratrace range.
PTFE capillaries	PTFE capillaries are transparent and enable visual tracing of the liquids to
(poly(tetrafluoroethy-	be pumped. They are chemically inert, flexible and temperature-resistant
lene))	up to 80 °C. They can be readily cut down to the desired length with the

capillary cutter (6.2621.080). Use:

PTFE capillaries (6.1803.0x0) are used for the low-pressure section.

PTFE capillaries with an inner diameter of 0.5 mm for sample processing.

PTFE capillaries with an inner diameter of 0.97 mm for sample processing and rinsing solutions (they are not necessarily included in the scope of delivery of the instrument).

Capillary connections

NOTE

If you work with an increased system pressure (> 15 MPa), capillaries may slip out of the pressure screws.

To avoid this, we recommend degreasing the ends of the capillaries before installing them.

Dampen a cloth with acetone and wipe off the ends of the capillaries before connecting them with the pressure screws.

In order to achieve optimum analysis results, capillary connections in an IC system must be absolutely tight and free of dead volume. Dead volume occurs if two capillary ends connected to each other do not fit exactly, thus allowing liquid to escape. There are two possible causes for this:

14	930 Compact IC Flex SeS (2.930.1400)

3 Installation

The capillary ends do not have exactly flat edges.

The two capillary ends do not completely meet.

One prerequisite for dead-volume-free capillary connection is that both capillary ends are cut exactly flat. Therefore we recommend cutting PEEK capillaries only with a capillary cutter (6.2621.080).

Also see: Cutting capillaries video on the Internet http://ichelp.metrohm.com/maintenance.php?chapter=1_1.

Creating dead-volume-free capillary connections

To create dead-volume-free capillary connections, proceed as follows:

1Wipe off the end of the capillary with a cloth dampened with acetone.

2Slide the pressure screw over the capillary. Ensure that the capillary protrudes 1 to 2 mm from the tip of the pressure screw.

3Push the capillary into the connection or coupling as far as it will go and hold it there.

4 Only then start turning the pressure screw.

Colored sleeves for PEEK capillaries

The enclosed set of varicolored sleeves for PEEK capillaries (6.2251.000) serves to easily differentiate the various flows of liquid in the system through color coding. Each capillary conveying a given liquid (e.g. eluent) can be marked with sleeves of the same color.

1Slide a sleeve of a selected color over a capillary and move it to an easily visible position.

2Heat the colored sleeve, such as with a hairdryer.

The colored sleeve shrinks and adapts to the shape of the capillary.

NOTE

In order to arrange capillaries more clearly, they can be bundled with the spiral band (6.1815.010).

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15

3.3 Removing transport locking screws

3.3Removing transport locking screws

	To avoid damage to the drives for the high-pressure pump and the vac-
	uum pump during transport, the pumps are secured with transport lock-
	ing screws. These are located at the rear of the instrument and labeled
	with Transport security screws.
	Remove these transport locking screws before the initial start-up.
Accessories	For this step you need:
	Hex key 4 mm (6.2621.030)
	Remove the transport locking screws

Figure 6 Removing the transport locking screws

1	Transport locking screws		2	Transport locking screws
	For the vacuum pump. Only present if the			For the high-pressure pump.
	instrument has a degasser.

1 Remove all of the transport locking screws with the hex key.

Store the transport locking screws in a safe place. Reinsert the transport locking screws each time you transport the instrument a significant distance.

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3 Installation

CAUTION

The pumps may be damaged if you transport the instrument without inserting the transport locking screws.

3.4Connecting the drainage tubing and leak sensor

The leak sensor detects leaking liquid that collects in the instrument's base tray. Liquid that leaks in the bottle holder or in the detector chamber is routed to the base tray using drainage tubing and is detected there.

If the leak sensor detects a leak in the IC system, the IC instrument is switched off and a warning is output in the software.

The leak sensor functions properly only if the following preconditions are met:

The drainage tubing is connected.

The leak sensor connection cable is inserted into the leak sensor connection socket.

The 930 Compact IC Flex is switched on.

The leak sensor is switched to active in the software.

3.4.1Installing the drainage tubing

Liquid that leaks in the bottle holder or detector chamber flows to the rear of the instrument and is drained through openings on the bottle holder and in the detector chamber. The drainage tubing has to be mounted at these openings. This drainage tubing guides the leaking liquid to the base tray where the leak sensor is located.

Accessories	For this step you need the following parts from the accessory kit: Vario/
	Flex Basic (6.5000.000):
		2 × silicone tubing (6.1816.020)
		Y connector (6.1807.010)

You also need scissors.

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17

3.4 Connecting the drainage tubing and leak sensor

Connecting the drainage tubing

1Cut a piece of silicone tubing into three pieces using scissors: 2 × approx. 40 cm and 1 × 20 cm

2Attach one end of the 40 cm long piece to the drainage tubing connection on the bottle holder.

3Attach one end of the 20 cm long piece to the drainage tubing connection on the detector chamber.

4Attach each of the loose ends of both pieces of silicone tubing to one end of the Y connector.

5Attach one end of the second 40 cm long piece to the third end of the Y connector.

Attach the loose end to the right-side drainage tubing connection on the base tray.

6Attach one end of the second piece of silicone tubing to the left-side drainage tubing connection on the base tray.

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3 Installation

Route the loose end into a waste container.

3.4.2Connecting the leak sensor

Plugging in the leak sensor connection cable

The leak sensor connection cable is coiled up in the base tray.

1Pull the leak sensor connection cable out of the base tray as far as needed.

2Plug the plug for the leak sensor connection cable into the leak sensor connection socket (labeled Leak Sensor).

3.5Connecting the eluent bottle

	The eluent is aspirated out of the eluent bottle via the eluent aspiration
	tubing . The eluent aspiration tubing is installed on the input for the high-
	pressure pump.
	The tubing has to be be fed out of the instrument through a suitable
	opening (see "Openings on the back panel", page 11) before the loose
	end can be connected to the eluent bottle.
Accessories	For this step you need the following accessories:
	These parts are part of the accessory kit Vario/Flex ONE (6.5000.010).
	Eluent bottle (6.1608.070)

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19

3.5 Connecting the eluent bottle

The eluent bottle cap GL 45 accessory set (6.1602.160)

This accessory set contains the bottle cap, an M6 tubing nipple, an M8 tubing nipple, two O-rings and an M6 and M8 threaded stopper.

The tubing adapter for aspiration filter accessory set (6.2744.210) This accessory set contains a filter holder, a clamping screw and tubing weighting.

An aspiration filter (6.2821.090)

Connecting the eluent aspiration tubing

1Installing the eluent bottle cap (6.1602.160)

Start by pushing the M8 tubing nipple onto the loose end of the eluent aspiration tubing, followed by the O-ring.

Push the loose end of the eluent aspiration tubing through the M8 opening of the bottle cap and screw it on for the time being.

Figure 7 Installing the eluent bottle cap

2Mounting the tubing adapter and the aspiration filter

Install the parts of the tubing adapter for aspiration filter

(6.2744.210) accessory set:

Start by pushing the tubing weighting onto the loose end of the eluent aspiration tubing.

Then push the clamping screw onto the loose end of the eluent aspiration tubing.

Lastly, push the filter holder onto the loose end of the eluent aspiration tubing and screw it onto the tubing nipple.

The end of the tubing should extend approximately 1 cm.

Installing the aspiration filter:

NOTE

Only handle the aspiration filter while wearing gloves.

20	930 Compact IC Flex SeS (2.930.1400)

3 Installation

Place the loose end of the eluent aspiration tubing into the aspiration filter.

The end of the tubing should reach approximately to the center of the aspiration filter.

Tighten the aspiration filter to the filter holder.

Figure 8 Installing tubing weighting and aspiration filter

3Installing the eluent bottle cap on the eluent bottle

Insert the eluent aspiration tubing into the eluent bottle (6.1608.070).

Tighten the bottle cap on the eluent bottle.

Adjust the length of the eluent aspiration tubing so that the aspiration filter is at the bottom of the eluent bottle. Then fasten it in place using the M8 tubing nipple.

Seal the M6 opening on the bottle cap with the M6 threaded stopper from the accessory set.

930 Compact IC Flex SeS (2.930.1400)

21

3.5 Connecting the eluent bottle

4 Mounting the adsorber tube

NOTE

Depending on the eluent used, the adsorber tube (6.1609.000) must be filled differently:

For alkaline eluents or eluents with a low buffer capacity: first a little cotton, then CO2 adsorber material.

For all other eluents: only cotton.

Remove the plastic cover from the large opening of the adsorber tube. Fill the adsorber tube and close it again using the plastic cover.

Insert the adsorber tube into the bottle cap's large opening. Fasten it to the bottle cap using the SGJ clip (6.2023.020).

22	930 Compact IC Flex SeS (2.930.1400)