Olympus IX51 User Manual [ru]

INSTRUCTIONS

IX71/IX51

INVERTED RESEARCH MICROSCOPE/ INVERTED BASIC MICROSCOPE

This instruction manual is for the Olympus Inverted Microscopes Models IX71 and IX51. To
ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of the microscope, we recommend that you study this manual thoroughly before operating
the microscope. Retain this instruction manual in an easily accessible place near the work
desk for future reference.	A X 7 3 1 9

IX71/IX51

CONTENTS

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read Chapter 9, “ASSEMBLY” (pages 55 to 65) carefully. For the modules provided with instruction manuals, also read the assembly procedures in their instruction manuals.

IMPORTANT — Be sure to read this section for safe use of the equipment. —		1-4
1	MODULE NOMENCLATURE	5
2	CONTROLS	6-11

3TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE 12, 13

4	USING THE CONTROLS					14-31
	4-1	Power Supply Unit and Microscope Frame.........................................................................................				14-16
		1	Turning Power On, Adjusting the Brightness	2	Light Path Selection
		3	Magnification Change (IX71 Only)	4	Frame Clamping Plate
		5	Option Button (IX71 Only)
	4-2	Focusing Block.............................................................................................................................................................................				16, 17
		1	Rotation Direction of the Coarse/Fine Adjustment Knobs
		2	Adjusting the Coarse Adjustment Knob Tension
		3	Detaching the Fine Adjustment Knob	4	Pre-focusing Lever
	4-3	Stage ...........................................................................................................................................................................................................				18, 19
		1	Placing the Specimen	2	Moving the Specimen
		3	Connecting the Grounding Wire	4	Adjusting the X-Axis/Y-Axis Knob Rotation Tension
	4-4	Observation Tube ....................................................................................................................................................................				20-22
		1	Adjusting the Interpupillary Distance	2	Adjusting the Diopter
		3	Using the Eye Shades	4	Using Eyepiece Micrometer Disks
		5	Selecting the Light Path of Observation Tube (U-TR30H-2 Only)
		6	Using the CT Turret (U-BI90CT Only)	7	Adjusting the Tilt (U-TBI90 Only)
	4-5	Illumination Column IX2-ILL100...........................................................................................................................				22-24
		1	Tilting the Illumination Column	2	Mounting the Filters
		3	Using the Field Iris Diaphragm
		4	Adjusting the Condenser Height Adjustment Knob Tension
		5	Mounting the Manipulator
	4-6	Illumination Column IX2-ILL30 .............................................................................................................................				25, 26
		1	Using the Aperture Iris Diaphragm	2	Removing the Condenser Lens
		3	Mounting the Filters	4	Using the Filters
	4-7	Condenser ..........................................................................................................................................................................................				27-29
		1	Centering the Condenser	2	Using the Aperture Iris Diaphragm
		3	Flipping Up the Condenser Holder
	4-8	Objectives ..........................................................................................................................................................................................				30, 31
		1	Oil-Proof Cap (UIS Series only)	2	Adjusting the Correction Collar
		3	Using Immersion Objectives

	5	OTHER OBSERVATION METHODS		32-43
		5-1	Phase Contrast Observation (Using the IX2-ILL100 Column) .................................	32-35
		5-2	Phase Contrast Observation (Using the IX2-ILL30 Column)....................................	36, 37
		5-3 Differential Interference Contrast Observation (Using the IX2-ILL100 Column) ....		38-42
		5-4	Simplified Polarized Light Observation (Using the IX2-ILL100 Column)..............	43
		5-5	Reflected Light Fluorescence Observation (See Separate Manual) ........................	43
	6	PHOTOMICROGRAPHY AND TV OBSERVATION		44-48
		6-1	Photomicrography ..................................................................................................................................................................	44-46
		6-2	TV Observation ............................................................................................................................................................................	47, 48
	7	TROUBLESHOOTING GUIDE		49-51
	8	SPECIFICATIONS		52-54

9ASSEMBLY — See this section for the replacement of the light bulb. — 55-65

PROPER SELECTION OF THE POWER SUPPLY CORD ...................................................................	66, 67
10 LAMP HOUSING INSPECTION SHEET	68

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

IX71/IX51

IMPORTANT

This microscope employs a UIS2/UIS (Universal Infinity System) optical design, and should be used with modules designed for the IX2 or BX2 series (as well as some of the modules designed for the Olympus IX or BX series).

For the applicable modules, please consult Olympus or the latest catalogues. Less than optimum performance may result if inappropriate module combinations are used.

If you want to use one or more motorized modules, you should prepare the IX2-UCB control box, U- HSTR2 hand switch, a PC and the IX2-BSW (Ver. 01.03) software (which runs on Windows 2000 or Windows Me).

Configuration of Instruction Manuals

Since these microscopes are expandable to a variety of systems, separate instruction manuals are prepared so that the user has to read only the manuals according to the user’s own system.

Manual Name	Main Contents
IX71/IX51	Observation procedures including transmitted light brightfield,
	phase contrast and DIC observations
Fluorescence System for IX2	Reflected light fluorescence observation
IX2-GCP	Glass Center Plate
IX2-MLWCD	Mid-Long Working Distance Condenser
IX2-DICD	DIC Condenser
U-FWT/FWR/FWO	Motorized filter wheels (The U-FWT cannot be used with these
	microscopes.)
IX2-UCB/U-HSTR2	Functions of the Control Box (incorporating the power supply)
	and Hand Switch
IX2 Software for PC (CD-ROM)	Methods of PC control of microscope functions
IX2-BSW (Ver. 01.03 or later)

Precautions When Unpacking the Microscope

³			Releasing the Transport Lock of the Revolving Nosepiece
			#Never attempt to rotate the coarse or fine adjustment knob @ without
				removing the clamping rod. Otherwise, the focusing mechanism may
				be damaged.
			1.	Loosen the screw ³ of the clamping rod ² using the Allen screwdriver
		|		provided with the microscope frame.
			2.	Rotate the coarse and fine adjustment knobs @ in the direction of the
				arrow and remove the clamping rod ².
			3.	Attach the provided seal (10 mm dia., black) on the hole made after remov-
²	@			ing the transport lock knob to prevent penetration of dust through the hole.
			}Retain the clamping rod ² and screw ³ carefully because they will be
	Fig. 1			used again the next time the microscope is transported.

Releasing the Transport Lock of the Light Path Selector

#Never attempt to operate the light path selector without removing the transport lock knob |. Otherwise, the light path selector mechanism may be damaged.

·Rotate the knob counterclockwise to remove it.

·To prevent penetration of dust through the hole made after removing the transport lock knob, stop the hole by attaching the provided seal (10 mm

dia., black).

}Retain the knob carefully because it will be used again the next time the microscope is transported.

Stage (IX2-SFR, IX-MVR)

·Before transporting the stage, fix the flexible knobs with pieces of adhesive tape so that they will not move.

1

SAFETY PRECAUTIONS

1.After the equipment has been used in an observation of a specimen that is accompanied with a potential of infection, clean the parts coming in contact with the specimen to prevent infection.

·Moving this product is accompanied with the risk of dropping the specimen. Be sure to remove the specimen before moving this product.

·In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures.

2.The microscope is provided with a simplified waterproof mechanism. Therefore, if culture liquid or water is spilt on the stage, revolving nosepiece or microscope frame, damage to the equipment or an electrical shock may result. Immediately wipe the liquid or water off if it is spilt on them.

3.When moving the microscope, remove the observation tube, condenser

		and reflected light mercury lamp housing, then carefully carry the micro-
		scope frame by the base (front edge) @ and the grasping part on the
		illumination column ² as shown in Fig. 2. (Weight: approx. 20 kg)
		Also be sure to remove the specimen since it may fall.
		When moving the microscope for a long distance, it is also recommended
	²	to disconnect all cables from the equipment.
		When transporting it, also engage the transport lock mechanisms and
		package it sufficiently.
	@	Also be careful against slipping of hands during carrying.
		#Damage to the microscope will occur if you grasp it by other parts
	Fig. 2	including the stage, coarse/fine adjustment knobs, etc.

4.The microscope is not covered by warranty in terms of laser safety. The user should assume liabilities for any consequence of user modification including introduction of the use of laser beam.

5.The surfaces of the lamp housing will become extremely hot during operation. When installing the microscope, make sure to allow ample free space (10 cm or more) around and in particular above the lamp housing.

6.When installing the microscope, route the power cord away from the lamp housing. Should the power cord come in contact with the hot lamp housing, the power cord could melt and cause electric shock.

	IX2-ILL100	7. To avoid potential shock hazards and burns when replacing the light
		bulb, set the main switch to “ ” (OFF) then disconnect the power cord
		from the wall outlet in advance. Whenever you replace the bulb during
	³	use or right after use, allow the lamp housing ³ and bulb to cool before
		touching. (Fig. 3)
		Designated halogen bulb	12V100WHAL (PHILIPS 7724)
		(Illumination column: IX2-ILL100)	Bulb life: 2000 hours of rated operation
		Designated halogen bulb	6V30WHAL (PHILIPS 5761)
		(Illumination column: IX2-ILL30)	Bulb life: 100 hours of rated operation

	Fig. 3	8. Always use the power cord provided by Olympus. If no power cord is
		provided, please select the proper power cord by referring to the section
		“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
		this instruction manual. If the proper power cord is not used, product
		safety performance cannot be warranted.
		9. Always ensure that the grounding terminal of the microscope and that of
		the wall outlet are properly connected. If the equipment is not grounded,
		Olympus can no longer warrant the electrical safety performance of the
		equipment.
		10. Never insert metallic objects into the air vents of the microscope frame
2		as this could result in electrical shock, personal injury and equipment
		damage.

IX71/IX51

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner.

Symbol	Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Warnings

Warning engravings are placed at parts where special precaution is required when handling and using the microscope. Always heed the warnings.

Warning engraving	Lamp housing	(High Temperature
position	(U-LH100L-3, U-LH100-3, IX-HLSH100, U-LS30-3)	warning)

1Getting Ready

1.A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.

2.Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For operating conditions, see Chapter 8, “SPECIFICATIONS” on page 54).

3.An intermediate attachment with a thickness of up to 60 mm can be mounted between the microscope frame and binocular observation tube (U-BI90CT, U-BI90).

·If the U-TBI90 is used together with an intermediate attachment, the image may be cut off or obscured.

4.The oil-proof cap can only be mounted on a UIS Series 3 objective. Note that this does not change the optical performance. (For applicable objectives, see page 30.)

5.Restrictions in brightfield, phase contrast and DIC observations

·When the U-BI90CT is used together with the optional 2X magnification changer built into the microscope frame, the brightness in the peripheral area of the field may become insufficient.

(The insufficiency in brightness can be improved by extending the light path length using the U-EPA2 eye-point adjuster.)

·With the combination of U-TR30H + U-FWO, the full optical performance may not be able to be manifested with the objectives listed in @ below and it is not possible to use the objectives listed in ² below. (This also applies to objectives other than Series 3 objectives.)

UIS2 Series

1UPlanSApo4X, 10X2, UPlanFLN4X, 10X2, 20X, CPlanFLN10X, LUCPlanFLN20X, UPlanSApo100XO

2PlanN40X, PlanApoN60XO

UIS Series

@UPlanApo4X, 10X, UPlanFl4X, 10X and 20X, CPlanFl10X, LCPlanFl20X, UPlanApo100XOI3 ²Plan40X, UPlanApo40XOI3/340, PlanApo60XO3

3

6.Restrictions in TV observation

@The following combinations are not permitted in consideration of the optical performance.

·IX2-SPT + PE4X + U-PMTVC on the side port

·IX2-SPT + PE5X + U-PMTV1X on the side port (With the IX71, this is applicable only when the magnification changer is set to 1X.)

·U-TV0.35XC + 2X magnification changer (optional)

·U-TV0.35XC + DP50 (optional 2X magnification changer on the side port)

²The following combination may deteriorate the optical performance a little.

·U-TV0.35XC + DP50 (UPlanSApo/UPlanApo4X or 10X on the side port) (With the IX71, this is applicable only when the magnification changer is set to 1X.)

7.Restrictions in fluorescence observation

·With combination of IX2-SHA + U-FWR (x 2) + U-LH100HGAPO, objectives UPlanSApo/UPlanApo40X, UPlanFLN/ UPlanFl20X, UPlanFLN100XO/100XOI, UPlanFl100XO3, PlanAPoN60XO and PlanApo60XO3 cannot be used due to a problem in the optical performance. (This also applies to objectives other than Series 3 objectives.)

8.Other

·The U-TRU or U-TVCAC cannot be mounted on the side port.

·When a large module is attached to the U-TR30H straight photo tube, it will be difficult to confirm the specimen.

·Only either the lower back port or left side port can be used.

2Maintenance and Storage

1.To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.

Since the absolute alcohol is highly flammable, it must be handled carefully.

Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical equipment that is being switched on or off.

Also remember to always use it only in a well-ventilated room.

2.Be sure to clean the oil immersion objective after use. Leaving immersion oil on it will degrades its performance.

3.Do not attempt to use organic solvents to clean the non-optical components of microscope. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

4.Never attempt to disassemble any part of the microscope.

5.When not using the microscope, make sure to set the main switch to “” (OFF), confirm that the lamp housing is cool enough and cover the microscope with the provided dust cover.

3Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment. } : Indicates commentary (for ease of operation and maintenance).

4Intended use

This instrument has been designed to be used to observe magnified images of specimens in routine and research applications.

Do not use this instrument for any purpose other than its intended use.

4

IX71/IX51

MODULE NOMENCLATURE

}The modules shown below are only the representative modules. As there are other modules which can be combined with the microscope but are not shown below, please also refer to the latest Olympus catalogues or your dealer.

For the modules marked *, refer to their separate instruction manuals.

Modules marked } are motorized modules, which should be used in combination with the IX2-UCB, a PC, etc.

Condenser

·IX2-LWUCDA2}

·IX2-LWUCD

·IX-ULWCD

·IX2-DICD*

·IX2-MLWCD*

·U-UCD8*

(Used together with IX-ADUCD)

Stage

·IX2-SFR

·IX-SVL2

· IX2-GS*

· IX2-SP

(Can be used together with IX-MVR)

· IX2-KSP

(Can be used together with CK40-MVR)

Eyepieces

· WHN10X · WHN10X-H

Observation Tube

· U-BI90CT

· U-BI90

· U-TBI90

· U-TR30H-2

(Used together with IX-ATU)

· U-MO

6-Position Revolving

Nosepiece

(Fixed on the microscope frame)

Microscope Frame

· IX71S1F-3 · IX71S8F-3 · IX51S1F-3 · IX51S8F-3

Fluorescence Mirror Unit

Cassette

·IX2-RFACA*}

·IX2-RFAC*

Hand Switch

U-HSTR2*}

Transmitted Light Lamp Housing

·U-LH100L-3

·U-LH100-3 + U-RMT

·IX-HLSH100 + U-RMT

Transmitted Light Illumination Column

·IX2-ILL100

·IX2-ILL30

Reflected Light Fluorescence Illuminator

·IX2-RFA*

·IX2-RFAL*

Reflected Light High-Intensity

Lamp Housing

·U-LH100HG*

·U-LH100HGAPO*

·U-LH75XEAPO*

High-Intensity Power

Supply Unit

· U-RFL-T* · U-RX-T*

Control Box

IX2-UCB*}

Power Supply Unit

· TH4

· TL4

PC}

(IX2-BSW Ver. 01.03 or later software installed)

5

CONTROLS

}If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 55 to 65).

· The illustration shows the system composed of modules enclosed in

.

IX71

Filter pocket (Page 23)

Field iris diaphragm lever

Condenser centering knobs

Universal Condenser

IX2-LWUCD

Aperture iris diaphragm lever

Tilting Binocular

Tube

U-TBI90

Indicator plate pockets

Light path selector lever (Page 15)

: Observation/

: Side port

Option button (Page 16)

Transmitted light ON-OFF button (Page 14)

Light intensity control knob (Page 14)

Coarse adjustment knob tension adjustment ring (Page 16)

Coarse adjustment knob (Page 16)

Fine adjustment knob (Page 17)

Detachable.

X-axis knob (Page 19)

Y-axis knob (Page 19)

Magnification selector knob (Page 15)

Pushed in: 1X Pulled out: 1.6X

Halogen Lamp Housing

U-LH100L-3

Applicable halogen bulb: 12V100WHAL-L

Illumination Column

IX2-ILL100

Condenser height adjustment knob (Page 27)

Column tilt clamping screw (Page 22)

Stage center plate

Cross Stage

IX2-SFR

Option connector (Page 16)

Microscope

Frame

IX71S1F-3

IX71S8F-3

Power Supply Unit

TH4

Light intensity control knob (Page 14)

Main switch (Page 14)

6

IX71/IX51

IX51

Lamp Socket

U-LS30-3

Filter holder (Page 26)

Applicable halogen bulb: 6V30WHAL

Aperture iris diaphragm lever (Page 25)

Phase Contrast Slider

IX2-SL

Diopter adjustment ring (Page 20)

Binocular

Observation

Tube

U-BI90

Indicator plate pocket

Microscope Frame

IX51S1F-3

IX51S8F-3

Coarse adjustment knob tension adjustment ring (Page 16)

Coarse adjustment knob (Page 16)

Fine adjustment knob (Page 17)

Detachable.

Light path selector lever (Page 15)

: Observation/ : Side port

Illumination Column

IX2-ILL30

Plain Stage

IX2-KSP

Mechanical Stage

CK40-MVR

Y-axis knob (Page 19)

X-axis knob (Page 19)

Power Supply Unit

TL4

Main switch (Page 14)

Light intensity control knob (Page 14)

7

Left Side View of Microscope Frame

IX71

Allen screwdriver

storage position (Rear panel)

Side port

Transport lock knob

#Be sure to remove this before use.

IX51

Allen wrench and optical element centering knob (x 2)

storage position (Rear panel)

Eyepieces

WHN10X/WHN10X-H

Binocular Observation Tube

U-BI90CT

Diopter adjustment ring (Page 20)

Interpupillary distance adjustment scale (Page 20)

CT turret (Page 21)

Focus ring (Page 21)

Coarse adjustment knob (Page 16)

Pre-focusing lever (Page 17)

Fine adjustment knob (Page 17)

Detachable.

Allen wrench and optical element centering knob (x 2)

storage position (Rear panel)

Eyepieces

Allen screwdriver

WHN10X/WHN10X-H

storage position (Rear panel) Binocular Observation Tube U-BI90CT

Diopter adjustment ring (Page 20)

Interpupillary distance adjustment scale (Page 20)

CT turret (Page 21)

Focus ring (Page 21)

Side port

Coarse adjustment knob (Page 16)

Pre-focusing lever (Page 17)

Transport lock knob

#Be sure to remove this before use.

Fine adjustment knob (Page 17)

Detachable.

8

IX71/IX51

Other Modules

Monocular Tube

U-MO

Plain Stage

IX2-KSP

Plain Stage

IX2-SP

Cross Stage

IX-SVL2

Y-axis knob

10X eyepiece

(Fixed)

Manipulator mounting screw holes

M6 screws (x 6)

CK40-MVR/CK2-SS mounting screw holes

(On both sides)

Manipulator mounting screw holes

M6 screws (x 6)

IX-MVR/CK2-SS mounting screw holes

(On both sides)

Trinocular Observation Tube H

U-TR30H-2

Straight photo tube mount (Page 44)

Light path selector knob (Page 21)

Intermediate Tube

IX-ATU

	Mechanical Stage	}Can be mounted on the IX2-KSP.
	CK40-MVR

	Mechanical Stage	}Can be mounted on the IX2-SP.
	IX-MVR

Y-axis knob

X-axis knob

X-axis knob

9

	Long WD Universal Condenser		UCD Adapter
	IX2-LWUCD		IX-ADUCD

}This is a manually controlled condenser.

	Aperture iris	Condenser height fine
		adjustment knob (Page 28)
	diaphragm lever
	(Page 27)	Turret (Page 27)
	Index mounting holes
		Centering knob insertion holes
		(x 2)

ULWCD Condenser

IX-ULWCD

}This is a manually controlled condenser.

	Aperture iris	Index mounting holes
	diaphragm lever	(x 2)
	(Page 27)
		Turret (Page 27)

Universal Condenser

U-UCD8

}This is a manually controlled condenser. Refer to the separate instruction manual for details.

Centering knob insertion hole

Motorized Long WD Universal Condenser

IX2-LWUCDA2

}This is a motorized condenser.

Index mounting holes

Centering knob insertion holes

Aperture iris diaphragm lever (Page 27)

10

IX71/IX51

Power Supply Unit

TH4

}The applicable halogen bulb is the 12V100WHAL-L. For details, refer to the separate instruction manual.

Hand Switch TH4-HS

}The IX71 does not need this module because its functions are incorporated in the microscope frame.

		Light intensity control
		knob
	POWER LED
Main switch	REMOTE LED	Lamp ON-OFF
: ON. : OFF.		switch
	Light intensity control knob

	Power Supply Unit	}The applicable halogen bulb is the 6V30WHAL.
	TL4

Light intensity control knob	Lamp socket
	connector

Main switch

: ON.: OFF.

11

TRANSMITTED LIGHT BRIGHTFIELD

OBSERVATION PROCEDURE

}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic observation method of this microscope. The operating procedures for phase contrast observation and DIC observation will be described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 32.

For the fluorescence observation, refer to the separate instruction manual entitled “Manual/Motorized Reflected Fluorescence System”.

Set the main switch to “ I ” (ON).

Disengage the DIC slider and analyzer from the light path.

(Controls Used)	(Page)
@Main switch: “ I ” (ON)	(P. 14)
²Lamp ON-OFF button: ON	(P. 14)
³Light intensity control knob	(P. 14)

		Disengage the filter other than the		|Filter pocket	(P. 23/26)
		frost filter from the light path.
Select the (observation) light path with the				ƒLight path selector lever	(P. 15)
light path selector lever.
				…X-axis and Y-axis knobs	(P. 19)
Place the specimen on the stage.
				†Revolving nosepiece
Engage a 10X objective in the light path.
Bring the specimen into focus.				‡Coarse/fine adjustment knobs	(P. 16)
				³Light intensity control knob	(P. 14)
		Adjust the brightness.
				ŠBinocular observation tube	(P. 20)
		Adjust the interpupillary distance.		‰Diopter adjustment ring	(P. 20)
		Adjust the diopter.		‹Condenser height adjustment knob* (P. 27/
		Adjust the light axis.		28)
				ŒCondenser centering knobs*	(P. 27/28)
				™Field iris diaphragm lever*	(P. 24)
Engage the objective to be used in the light				†Revolving nosepiece
path and bring the specimen in focus.				‡Coarse/fine adjustment knobs	(P. 16)
				šAperture iris diaphragm lever	(P. 29)
Adjust the aperture iris and field iris diaphragms.
				™Field iris diaphragm lever*	(P. 24)
		Engage the required filters.		|Filter pocket	(P. 23/26)
		Adjust the brightness.		³Light intensity control knob	(P. 14)
Start observation.

* This operation is not required when the IX2-ILL30 illumination column is used.

12

IX71/IX51

|

™

Œ

š

‰

Š

ƒ

With the IX71

³

²

‡	Hand Switch
	TH4-HS

³

²

‹

†

ƒ

With the IX51

…

Power Supply Unit

TH4

@

³

When the TL4 power supply unit is used

³

@

} Make a photocopy of the observation procedure pages and post it near your microscope.

13

USING THE CONTROLS

4-1 Power Supply Unit and Microscope Frame

}The power supply unit to be used is variable depending on the illumination column in use.

·IX2-ILL100: TH4 power supply unit.

·IX2-ILL30: TL4 power supply unit.

		1	Turning Power On, Adjusting the			(Figs. 4 & 5)
			Brightness
			1.	Make sure that the light intensity control knob @ is in the MIN (minimum
				intensity) position and set the main switch ² to “ I ” (ON). (The POWER
		³		LED ³ lights up.)
			2.	Rotate the knob @ toward MAX (maximum intensity) to increase the
		|		intensity and the illumination brightness.
	²		}With the TH4, the position (approx. 9 V) marked indicates the position
		@
				where the daylight illumination suitable for photomicrography is obtained
		Fig. 4		when the 45LBD filter is engaged in the light path.
				The position marked of the IX71 and TH4-HS also has the identical
				function.
				With the TL4, the position marked	is not provided and even
				the maximum light intensity (6 V) may sometimes be unsuitable
				for photomicrography.

@

²

Fig. 5

…

ƒ

Fig. 6

With the IX71 Frame (Fig. 6)

}If the TH4 connection cable provided with the microscope frame is connected to the TH4 (the REMOTE LED | lights in this case), the light intensity control knob @ on the power supply unit is defeated and only the light intensity control knob ƒ on the front of the microscope frame is available.

1.Press the transmitted light ON-OFF button … (so that the button is illuminated) and adjust the brightness with the light intensity control knob ƒ.

2.To turn the lamp OFF, set the transmitted light ON-OFF button … to OFF.

#The microscope frame is in standby mode when the REMOTE LED | is lit. Power of about 2.5 W is consumed in this period.

When the microscope system is not be used for a long period, set the main switch ² to “” (OFF).

14

IX71/IX51

When the TH4-HS Hand Switch is Used (Fig. 7)

ƒ

}The illumination brightness can be adjusted from the hand switch in the same way as on the IX71 microscope frame.

The hand switch is provided with double-sided adhesive tape, so it can be adhered to an easy-to-use position.

…

Fig. 7

IX71

@

²

Fig. 8

2	Light Path Selection	(Figs. 8 & 9)
}The light path selector lever allows for light path switching between the
	observation ( ) and side port (	) paths.

: Observation 100% light path.

: Side port 100% light path (with the IX71S1F-2/IX51S1F-2 frame) Side port light path 80%/Observation 20% light path (with the IX71S8F-2/IX51S8F-2)

}The light path to the lower back port can also be set by the manufacturer’s part replacement operation. (IX2-LBPC)

IX51

@

Fig. 9

IX71		3 Magnification Change (IX71 Only)	(Fig. 10)
		When the magnification selector knob @ is pulled out, the magnification
		will be 1.6X. When the knob is pushed in, the magnification will be 1X.
		}The 1X 1.6X magnification changer lens can be replaced with a 1X 2X
	@	magnification changer lens (IX2-CA2) by the manufacturer operation.

Fig. 10

15

4Frame Clamping Plate

}This is the module for clamping the microscope frame onto an anti-vibration platform. The applicable anti-vibration platforms are the following four models

·25 mm pitch and 50 mm pitch anti-vibration platforms.

·1-inch pitch and 2-inch pitch anti-vibration platforms. For the assembly procedure, see item 10 on page 65.

5

Option Button (IX71 Only)

(Fig. 8)

}Pressing the option button ² makes it possible to release or close an external shutter (UNIBLITZ, etc.) To do this, the external shutter be connected to the right side of the frame using a commercially available BNC cable.

#When the option button is not used, leave the cap attached to it.

4-2 Focusing Block

²

@³

	1	Rotation Direction of the Coarse/	(Fig. 11)
		Fine Adjustment Knobs

}Rotating the coarse or fine focus adjustment knob @ toward the front (in the direction of the arrow) raises the objective and toward the rear (opposite direction) lowers the objective.

	2	Adjusting the Coarse Adjustment Knob	(Fig. 11)
		Tension

Fig. 11

#Always use the rotation tension adjustment ring ² to control the rotation tension of the coarse adjustment knob.

The tension of the coarse adjustment knob has been pre-adjusted to optimum tension, but this can be changed as required. Turn the rotation tension adjustment ring ² in the direction of the arrow to decrease the knob’s tension and in the opposite direction to increase it.

If the objective lowers by its own weight or the focusing obtained with the fine adjustment knob is lost soon, the tension is set too low. In this case, turn the rotation tension adjustment ring in the opposite direction to the arrow to increase the tension.

16

IX71/IX51

3
	Detaching the Fine Adjustment Knob	(Fig. 11)

}The fine adjustment knob is designed detachable in order to prevent interference between the knob and the operator’s hand manipulating the X- and Y-axis knobs.

·Loosen the clamping screw ³ using the Allen screwdriver and remove the fine adjustment knob.

After detaching, the seat of the fine adjustment knob is hollowed to facilitate manipulation with the thick of a finger.

	4	Pre-focusing Lever	(Fig. 12)
		}The pre-focusing lever prevents collision between the specimen and
		objective and simplifies the focusing operation.
		After bringing the specimen into approximate focus with the coarse
		adjustment knob, turn the pre-focusing lever @ in the direction of the
		arrow to lock it. Hereafter, the upper limit of the coarse adjustment will
		be limited at the position where the lever is locked.
	@	When bringing a specimen in focus, approximate focus can be obtained
		by simply raising the coarse adjustment to the stop position so all you
	Fig. 12	have to do more is control the fine adjustment knob.
		}The stage up/down movement using the fine adjustment knob is not
		limited.
		#When the pre-focusing lever is locked, the coarse adjustment stroke is

limited by the mechanism and it cannot reach the previous lower limit. If you want to control the coarse adjustment knob to the previous lower limit, unlock the pre-focusing lever.

17

4-3 Stage

@	1 Placing the Specimen

(Figs. 13 to 16)

With the IX2-SFR or IX-SVL2 Stage	(Fig. 13)

Place the specimen on the center of the stage.

}In the case of a slide glass specimen, place the specimen with the cover glass facing down.

}If the specimen is prone to slide on the stage, attach the stage clips (IX-SCL) @ and clamp the specimen down with the clips.

Fig. 13

@

Fig. 14

With the IX-MVR Mechanical Stage + IX2-SP Stage (Figs. 14 & 15)

}96-well or 24-well microtiter plates, etc. are held in place by the specimen holder.

Microtiter plates with dimensions of max. 136 mm x 92 mm can be accommodated in this way.

1.Open the spring-loaded finger of the specimen holder @ and slide the microtiter plate into the holder frame. Gently release the curved finger to clamp. (Fig. 14)

		}To secure other vessels than microtiter plates, various optional holders
	²		are available. A Terasaki plate holder ² is available for holding Terasaki
	³		plates (72-well, or 60-well). When using this, it is necessary to replace the
			stage scales with those provided with the plate holder. Petri dish holder
	|
			³ is available for 35 mm, 54 mm and 65 mm diameter petri dishes, and
			a slide glass holder | is available for holding slide glass, and the IX2-
			BCTP* ƒ is available for a blood cell test plate holder. (Fig. 15)
		* A blood cell test plate or other calculating chamber for bacteria and
				+0.3
	ƒ		eosinophil with mounting section dimensions corresponding to H 77+0 x
			+0.3
			V 35+0 x D 2 mm can be used. A 60 mm diameter petri dish can also be
	Fig. 15		used.
		With the CK40-MVR Mechanical Stage + IX2-KSP Stage		(Fig. 16)
		1.	96-well or 24-well microtiter plates can be held directly in place by
	@		widening the specimen holder @.
		2.	To secure other vessels than microtiter plates, use one of the following
	²
			holders provided with the mechanical stage.
	³		· Terasaki plate holder ² (AB4488): For test plate, 35 mm diameter petri
			dish holder | or 65 mm diameter petri dish
	|		· Slide glass holder ³ (AB4489): For slide glass or 54 mm petri dish
		}The IX2-BCTP blood cell test plate holder (Fig. 15 ƒ) can also be used.

Fig. 16

18

IX71/IX51

	2 Moving the Specimen	(Fig. 17)
³	With the IX2-SFR or IX-SVL2 Stage

To move the specimen to a desired position, rotate the X-axis knob @ and Y-axis knob ².

}When the index mark on the upper stage is aligned with the index line ³ ² provided on the substages, the center of the stage center plate aperture is almost in the center of the optical axis. Use this as a guideline when

	@	moving the specimen.
	Fig. 17	The travel area is 50 mm (X-axis) x 50 mm (Y-axis).
		}If, after observing a specimen with an objective with short WD (0.5 mm
		or less), the revolving nosepiece is rotated to change the objective, the
		objective may interfere with the center plate. When using objectives
		with short WD or immersion objectives frequently by avoiding interference,
		it is recommended to attach the optional IX-CP50 center plate with a
		center aperture diameter of 50 mm.
		}The travel area of the IX-SVL2 stage is 50 mm (X-axis) x 43 mm (Y-axis).
		With the IX-MVR or CK40-MVR Mechanical Stage
		Specimens are moved in the same manner as outlined above.
		}The stage travel area is 130 mm (X-axis) x 85 mm (Y-axis) with the IX-MVR
		or 120 mm (X-axis) x 78 mm (Y-axis) with the CK40-MVR.

²	3	Connecting the Grounding Wire		(Fig. 18)
		With the IX2-SFR or IX-SVL2 Stage
		}A grounding wire can be attached to the stage for electrophysiological
@			experiments, etc.
			Prepare a grounding wire @ and one M4 screw ² and attach the
			grounding wire as shown in Fig. 18.
		#The screw hole may sometimes be stuck by paint, etc. In such a
			case, screw in the M4 screw a few times to expose the metallic
Fig. 18			thread inside the screw hole and improve the contact before
			attaching the grounding wire firmly.
@	4	Adjusting the X-Axis/Y-Axis Knob Rotation Tension		(Fig. 19)
		With the IX-SVL2 Stage
		}The tension of the X-axis and Y-axis knobs can be adjusted independently.
		1.	Loosen the two setscrews @ of a knob using the provided Allen wrench,
			hold the stage so that it will not move, then rotate the knob to adjust the
			tension. Rotating it in the direction of the arrow increases the tension and
			rotating in the opposite direction decreases the tension.
		2.	After adjustment, tighten the setscrews firmly.
Fig. 19		#If the tension of a knob is too tight or too loose, skipping or returning
			of image may occur during the stage movement.

19