EM AFS2
Leica EM AFS2
Automatic Freeze Substitution System
Leica EM FSP
Freeze Substitution Processor
The Leica EM AFS2 provides all the features for …
Perfect results:
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Reproducible
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Excellent specimen visibility with
– Internal LED illumination
– Stereomicroscope (optional)
•
-140 °C to +70°C working range
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“Deep Freeze” allows sample transfer at temperatures below -140 °C
•
Transfer function “TF” excludes humidity and oxygen
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Fast UV polymerization with LED UV lamp
Flexibility
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Any choice of substitution system, e.g.
– Substitution Capsule and Flat Embedding System
– New Microtube Embedding System EM AFS2
– Sapphire Disk FS System
•
Trolley for mobility
Ease of use
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Intuitive programming
– 10 users
– 99 programs each
– 99 program steps each, 0 to 99 hrs 59 mins
– graphic display of substitution protocol
– menu prompts
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Mouse controlled color screen
•
Memory stick to transfer programs
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Log file download on memory stick
Safety and convenience
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Operator safety due to fume exhaust system
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35 l Dewar
•
LN
2
filling from outside specimen chamber
•
LN
2
consumption enough for 5 day protocols
•
Stainless steel working platform
Leica EM AFS2
Freeze Substitution has never been easier…
Freeze Substitution (FS) of specimens in methanol, acetone or any other FS media at low tem-
peratures is THE follow-on procedure to high pressure freezing and other cryo fixation methods.
Progressive Lowering of Temperature (PLT) allows substitution and resin infiltration of
chemically fixed specimens.
Finally, the sample is polymerized under UV light in the EM AFS2 and can be cut and immuno
labelled.
LED UV lamp
FS chamber with new cryotube insert
2
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