Leica CM1950 User Manual

Leica CM1950

Cryostat

Operating Manual

Leica CM1950, V 1.1 English – 05/2007

Always keep this manual near the instrument.

Read carefully prior to working with the instrument!

1. NOTE

The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state- of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.

For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual.

Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products.

These are determined only by the contract provisions agreed between ourselves and our customers.

Leicareservestherighttochangetechnicalspecifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.

This document is protected under copyright laws. All copyrights to this documentation are held by Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument.

© Leica Biosystems Nussloch GmbH

Published by:

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Phone: [phone]

Fax: [phone]

eMail: [e-mail] Internet: http://www.histo-solutions.com

Leica CM1950 – Cryostat

3

Table of contents

1.	Important Information.........................................................................................................................		6
	1.1	Symbols Used in This Manual and Their Meaning ................................................................	6
	1.2	Qualification of Personnel..........................................................................................................	6
	1.3	Designated Use............................................................................................................................	6
	1.4	Instrument Type...........................................................................................................................	6

2.	Safety and Design................................................................................................................................		7
	2.1	General Safety Instructions .......................................................................................................	7
	2.2	Unpacking and Installation ........................................................................................................	7
	2.3	Safety Features..........................................................................................................................	10
	2.4	Securing/Locking the Handwheel ..........................................................................................	11
	2.5	Cleaning, Disinfection – Turning the Instrument Back On..................................................	12
	2.6	Handling Specimens – Defrosting ..........................................................................................	13
	2.7	Removal of the Microtome.......................................................................................................	13
	2.8	Maintenance ..............................................................................................................................	13

3.	Technical Data ...................................................................................................................................		14
4.	Standard Delivery ..............................................................................................................................		16
5.	General Overview..............................................................................................................................		18
	5.1	Control Panel Fields and Cryostat Chamber..........................................................................	19
6.	Installation ...........................................................................................................................................		20
	6.1	Site Requirements .....................................................................................................................	20
	6.2	Transport to the Site .................................................................................................................	21
	6.3	Assembling the Handwheel, Centering the Handwheel (Optional) ...................................	22
	6.3.1	Locking/Unlocking the Handwheel.........................................................................................	23
	6.3.2	Installing the Footswitch Dummy (Instruments with Cutting Motor) ................................	23
	6.4	Connection to Power Supply System .....................................................................................	24
	6.5	Installing Accessories/Inserting Chamber Accessories ....................................................	25
	6.5.1	Installing the Adjustable Footrest (Optional) ........................................................................	25
	6.5.2	Installing the Storage Systems (Optional) .............................................................................	26
	6.5.4	Inserting the Section Waste Tray ...........................................................................................	26
	6.5.3	Shelf, Movable (Optional).........................................................................................................	26
	6.5.5	Installing the Heat Conductor Block, Stationary (Optional) ...............................................	27
	6.5.6	Installing the Knife/Blade Holder and Adjusting the Clearance Angle.............................	27

4	Operating Manual V1.1 – 05/2007

7.	Instrument Controls...........................................................................................................................		30
	7.1	Control Panel Fields on the CM1950 – Control Panel Field 1 ..............................................	30
		Control Panel Field 2 – Electric Coarse Feed, Sectioning
		and Trimming Thickness Display ............................................................................................	32
		Control panel 3 – Motorized sectioning (optional) ...............................................................	34

8.	Working with the Instrument.........................................................................................................		36
	8.1	Preparing Cutting Tools, Specimen Discs and Preparation Aids ......................................	36
	8.2	Turning the Instrument On .......................................................................................................	36
	8.3	Configuring the Parameters.....................................................................................................	37
	8.4	Working with the Precooled Cryostat ....................................................................................	42
	8.4.1	Preparatory Work......................................................................................................................	42
	8.4.2	Trimming with Extraction – Anti-roll Guide Installed ...........................................................	43
	8.4.3	Cutting with Extraction – Anti-roll Guide Installed ...............................................................	45
9.	Troubleshooting .................................................................................................................................		48
	9.1	Problems During Work .............................................................................................................	48
10.	Temperature Selection Chart (in minus °C) ..............................................................................		52
11.	Optional Accessories .......................................................................................................................		53
	11.1	Ordering Information.................................................................................................................	53
12.	Maintenance and Cleaning ............................................................................................................		66
	12.1	General Maintenance ...............................................................................................................	66
	12.2	Changing Fuses..........................................................................................................................	67
	12.3	Replacement of the Lamp/UVC Lamp.....................................................................................	68
13.	Decontamination Certificate (master) ........................................................................................		70
14.	EC Declaration of Conformity ........................................................................................................		72
15.	Warranty and Service ......................................................................................................................		73
16.	Peoles Republic of China................................................................................................................		74

Leica CM1950 – Cryostat

5

1.Important Information

1.1Symbols Used in This Manual and Their Meaning

Warnings

appear in a gray box and are marked by a warning triangle.

Notes

i.e. important user information appear in a gray box and are marked by an information symbol.

Caution – UVC radiation!

(5)Figures in brackets refer to item num-

(Fig.5)	bers in drawings or to the drawings
	themselves.

1.2Qualification of Personnel

The Leica CM1950 may be operated by trained laboratory personnel only.

All laboratory personnel designated to operate the instrument must carefully read the present instruction manual prior to starting work with the instrument.

1.3Designated Use

The CM1950 is a high-performance cryostat with an encapsulated microtome and separate specimen cooling. It features a UV disinfection system, an (optional) integrated extraction system for section waste and an (optional) motor for motorized sectioning.

Thecryostatisdesignedtoproducefrozensections for biological, medical and industrial applications.

The CM1950 is suitable for in-vitro diagnostic (IVD) applications.

The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in this manual.

Any other use of this instrument is considered as improper operation.

1.4Instrument Type

All information given in this instruction manual applies only to the instrument type indicated on the title page.

A name plate, indicating the instrument serial number, is attached to the back of the instrument.

Despite chemical and/or UV-light disinfection, personal safety precautions as per the applicable laboratory regulations must still be taken (i.e. safety goggles, gloves, laboratory coat and mask must be worn).

This type of disinfection reduces the number of germs by at least 99.99%.

Fig. 1

6	Operating Manual V1.1 – 05/2007

2. Safety and Design

2.1General Safety Instructions

The CM1950 is a cryostat with an encapsulated microtome and separate specimen cooling. It is primarily used for work in the area of fast-cut diagnostics. The displays and instrument controls are easy to operate due to their largely self-explanatory symbols. LED displays make it easy to read. The cryochamber is made of seamlessly welded, high-quality stainless steel that is free of difficult-to-access corners and thus easy to clean and disinfect. This instrument has been built and tested in accordance with the following safety regulations on electrical measuring, control, regulating and laboratory devices. In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this instruction manual.

For current information about applicable standards, please refer to the CE declaration of conformity on our Internet site:

www.histo-solutions.com

2.2Unpacking and Installation

•To ensure proper function of the instrument, it must be set up with a minimum distance on all sides from walls and furniture (see ‘Site Requirements’, p. 20)

•The instrument may only be transported in an upright or slightly inclined position.

•To ensure a safe transport with a fork lift 3 people are required: one operating the fork lift, and the other 2 holding the instrument on either side to prevent it from sliding down.

•Before connecting to the power supply system, please check if the local voltage complies with the power rating specified on the name plate of the instrument (see also ‘Technical data’)!

•Never connect the instrument to a power socket that does not have a protective conductor terminal.

Length of power cable: up to 3.5m

extension possible: no

After transporting, wait at least 4 hours before turning the instrument on. This waiting period is necessary to allow the compressor oil, which may have been displaced during transport, to return into its original position. Any condensation on electrical parts that has formed due to temperature differences during transport must be allowed to dry completely. Failure to comply with this can cause severe damage to the instrument!

Leica CM1950 – Cryostat

7

2.Safety and Design

		Fig. 2
1	2	3	2

5

5

7

5

4

4

9

14

17

Cover = ramp

Fig. 3

When the instrument is delivered, check the tilt indicators on the packaging.

If the arrowhead is blue, the shipment was transported laying flat, was tilted at too great an angle or fell over during transport.

Note this on the shipping documents and check the shipment for possible damage.

8	8	9
		8
	8
8		10
	11	11

Opening the packaging

1.Remove the 3 screws (1) on each of the two sides of the box (7) at the top.

2.Remove the 2 screws on each of the 4 hinges (2) on the front and rear side of the box.

Do NOT remove the screws of the hinges on the top lid!

3.Remove 1 screw (3) between the two hinges on the front and rear side of the box.

4.Remove the 3 screws (4) at the bottom on all four sides of the box.

5.Of the two vertical rows of screws (5), remove one on either side (which side does not matter) entirely.

8	Operating Manual V1.1 – 05/2007

2. Safety and Design

Removing the packaging

66

66

Fig. 4

Ramp assembly

13

12

Fig. 5

1.Remove the cover (17) and place it on the floor with the foam parts facing downwards. - The cover will be used as a ramp later.

2.Remove the 4 foam parts (6) by pulling them upwards.

3.Open the box (7) on its side hinges like an accordion partition and remove it.

4.Remove the accessories carton (8) and operating manual on the front of the instrument.

5.Pull off the dust cover (9) by pulling upwards.

6.Take off the foam strip (10) on the bottom rear.

1.After the foam strip (10) is removed, 2 screws (12) are visible on the baseplate.

2.Swing out the cover hinges (2) at the front and rear.

3.Attach the cover (17) from the rear on the transport pallet (11).

The notches (13) of the hinges (2) must bepointingtowardsthe instrument.

4.Move the cover towards the left so that the notches (13) of the hinges slide beneath the heads of the screws (12).

Transport to the Installation Location

16

15

2 14

17

Fig. 6

The instrument must be transported in an upright position.

The cover (17) now forms a ramp on which the instrument can now be rolled down off the transport pallet.

Do not push the device by its hood (16). Use the transport grip

2points (15 ).

The rollers (14) must run over the cover hinges (2) at the front and rear. Danger of tipping!

1.Carefully roll the instrument backwards from the pallet.

2.Push the instrument to the installation location on the castors (14).

Leica CM1950 Cryostat

9

2.Safety and Design

2.3Safety Features

The instruction manual includes important information related to the operating safety and maintenance of the instrument.

The Operating Manual is an important part of the product, and must be read carefully before startup and use and must always be kept near the instrument.

If additional requirements on accident prevention and environmental protection apply in the country of operation, this Operating Manual must be supplemented by appropriate instructions to ensure compliance with such requirements.

The instrument is equipped with the following safety devices: an emergency stop switch (motorized instruments only), a handwheel lock and centering system (motorized instruments only), knife guard on the blade and knife holder, and a blade ejector.

To prevent adverse health effects from UV radiation, the UV disinfection cycle can be started only after the sliding window has been properly closed. Closing the window activates the corresponding safety features.

The consistent use of these safety features and strict observation of the warnings and cautions in this manual, will safeguard the operator from accidents and/or personal injury to a great extent.

Microtome knives

•Take care when handling microtome knives/disposable blades. The cutting edge is extremely sharp and can cause severe injury!

•Never leave knives and dismounted knife holders with a knife/blade mounted lying around!

•Do not place a knife on a table with the cutting edge facing upwards!

We strongly recommend using the safety gloves included with the standard delivery!

•Never try to catch a falling knife!

•Prior to manipulating the knife and specimen or changing the specimen, always lock the handwheel and cover the cutting edge with the knife guard!

•Avoid contact with cold parts of the instrument as this can cause frostbite!

10	Operating Manual V1.1 – 05/2007

2. Safety and Design

Knife guard

Prior to manipulating the knife and specimen, changing the specimen or knife, or taking a break, always lock the handwheel and cover the cutting edge with the knife guard.

The CE, CN and CN-Z knife holders feature knife guards; the glass anti-roll plate of the CE knife holder also serves as a knife guard.

2.4Securing/Locking the Handwheel

12 o’clock position

6 o’clock position

Fig. 7

1

2

Always lock the handwheel and cover the cutting edge with the knife guard prior to manipulating the knife or specimen, changing the specimen, or taking a break.

To lock the handwheel, press the lever (1) outward. Continue turning the handwheel slowly until the grip is in the upper or lower position and the handwheel is locked. Press the lever fully outward; gently rock the handwheel until the locking mechanism clicks into place noticeably.

To release the handwheel, press the lever (2) on the handwheel toward the cryostat housing.

Fig. 8

Fig. 9

Centering of the handwheel (motorized instruments only)

Pull out the handwheel’s handle and position it in the middle of the handwheel. The handle automatically engages in this position.

An important safety device on the cryostat is the centering of the handwheel for motorized instruments.

Rotate the handwheel only if the refrigeration system is on and the cryochamber is cold.

Leica CM1950 – Cryostat

11

2.Safety and Design

2.5Cleaning, Disinfection – Turning the Instrument Back On

It is not necessary to remove the microtome for disinfection.

•The instrument has been designed for UV disinfection! Spray disinfection with Leica Cryofect is also possible, thanks to the special insulation of the microtome. (Cryofect is not available in all countries!)

Remove section waste after EVERY sectioning operation and BEFORE changing specimens. Remove the section waste using a paper towel soaked in Cryofect or an alcohol-based disinfectant) or remove the waste using the extraction nozzle (optional). Do not start the disinfection until after folding the section stretcher to the side! Each new specimen is a potential source of contamination.

•When disinfecting the instrument, take appropriate protective measures (gloves, mask, protective clothing, etc.).

•When using detergents and disinfectants please comply with the safety precautions of the disinfectant manufacturer.

•The integrated glass anti-roll guide of the blade holder CE, CN and CN-Z can be cleaned either with acetone or alcohol.

•Dispose of waste liquid according to the waste disposal regulations!

•Do not use external heaters for drying the cryochamber. This can cause damage to the cooling system!

•Do not turn the instrument on before the cryochamber is completely dry! Frost formation!

•All components removed from the cryostat must be carefully dried before returning them to the cryochamber!

•The front panel and the slit cover of the microtome must be completely dry before turning on the instrument!

For more detailed information about disinfection, visit the Leica

Microsystems website at

www.histo-solutions.com

12	Operating Manual V1.1 – 05/2007

2. Safety and Design

2.6Handling Specimens – Defrosting

•When working with contaminated or infected material, the general safety guidelines for laboratories must be applied.

•Before defrosting the cryochamber remove all samples!

•Before defrosting the specimen head, remove all samples!

Never leave samples in the cryochamber! - The instrument is not suitable for storing frozen specimens, as the refrigeration dehydrates the specimens!

The quick freeze shelf can become very hot during the defrosting process. Do not touch it during defrosting.

2.7Removal of the Microtome

•The microtome is encapsulated and therefore does not require removal by the user.

2.8Maintenance

Replacing the fuses

•Turn the instrument off and disconnect the power plug before replacing the fuses.

•Use only the fuse types specified in chapter 3, "Technical Data." Failure to comply with this can cause severe damage to the instrument!

Replacing the fluorescent lamp/UVC lamp

•Turn the instrument off and disconnect the power plug before replacing the lamps.

It is possible to break the UVC lamp during replacement. If this happens, the lamp change must be completed by Technical Service. If any metallic mercury is released, handle it carefully and dispose of it properly.

If both disinfection indicator lights are blinking alternately, the

UV tube must be replaced!

Leica CM1950 Cryostat

13

3.Technical Data

All specifications related to temperature are valid only up to an ambient temperature of 18°C to 35°C and a relative humidity of no more than 60%.

Type	-1	-2	-3
Nominal voltage (±10 %)	100 VAC	120 VAC	230 VAC
Nominal frequency	50/60 Hz	60 Hz	50 Hz
Power draw	1900 VA	1900 VA	1900 VA
Max. start-up current for 5 sec.	35A eff.	35 A eff.	25 A eff.
Protective class	I	I	I
Circuit breaker:	15A M3	15A M3	15A M3
(MDA made by Schurter)
Pollution degree	2	2	2
Overvoltage installation category	II	II	II
Heat emission (max.)	1900 J/s	1900 J/s	1900 J/s
Approval	CE/c_CSA_us	CE/c_CSA_us	CE/c_CSA_us

according to IEC-1010; UL 3101

Microtome

Type:		Rotary microtome,
		encapsulated
Section thickness range		1 to 100μm
Trimming range:
Clinic		10 - 40μm
Research		1 - 600μm
Total specimen feed		25mm + 1mm
Vertical stroke		59mm ±0.5mm
Specimen retraction		20μm(canbedeactivated)
Maximum specimen size		50 x 80mm
Cutting speed		Slow: 0-50 strokes/min
		Fast: 0-85 strokes/min
		Vmax: 85-90 strokes/min
Specimen orientation		8° (x, y-axis)
For additional details, see p. 32
Electric coarse feed
Slow		300μm/s
Fast		900μm/s
	For "Site Requirements," refer to item 6.1
	(page 20).
Lamp
50/60 Hz-Version: Osram DULUX L 18W/840

Cryostat

Dimensions:
Width (w/o handwheel)	700 mm
Width (with handwheel)	835 mm
Depth (cabinet only)	850 mm
Height (total)	1215 mm
Working height (armrest)	1025 mm
Weight:
Weight (w/motor and suct.)	193 kg
Weight (w/motor, w/o suct.)	185 kg
Weight (w/o motor, w/ suct.)	183 kg
Weight (w/o motor, w/o suct.)	175 kg
Weight (w/ specimen head cool.)	165 kg
Weight (w/o specimen head cool.)	145 kg

General information

Operating temperature range: 18°C to 35°C Temperature range

during storage:	+5°C to +55°C
Relative humidity:	max. 60%,
	non-condensing
Storage humidity:	< 60%

14	Operating Manual V1.1 – 05/2007

		3.	Technical Data
Refrigeration system	CM1950, 50 Hz	CM1950, 60 Hz
Cryochamber
Temperature range	0°C to -35°C ± 5 K, adjustable in 1K increments,	0°C to -35°C ± 5 K, adjustable in 1K increments
	at ambient temperature of 20°C	ambient temperature of 20°C
Cooling time to -25°C	approx. 5 h	approx. 5 h
Cooling time to -35°C	approx. 8 h	approx. 8 h
Refrigeration capacity	690 W	690 W
Cut-out pressure	25 bar	25 bar
Safety factor	3	3
Refrigerant*	300 g (± 5 g) refrigerant R -404A*	300 g (± 5 g) refrigerant R -404A*
Compressor oil*	0.6 l EMKARATE RL22S, ICI*	0.6 l EMKARATE RL22S, ICI*
Defrosting of cryochamber
Automatic defrosting
Programmable:	Yes (hot gas defrost) Selectable time	Yes (hot gas defrost) Selectable time
Defrosting intervals	1 defrost in 24 h	1 defrost in 24 h
	or manual hot gas defrost	or manual hot gas defrost
Defrost time:	12 minutes	12 minutes
Automatic shutoff Defrost:	at -5 °C chamber temperature	at -5 °C chamber temperature
Quick-freeze shelf
Minimum temperature:	- 42 °C (+ 5 K), at chamber temp. -35°C	- 42 °C (+ 5 K), at chamber temp. -35°C
Number of freezing stations:	15+2	15+2
Defrost	Manual hot-gas defrost	Manual hot-gas defrost
Peltier element
Number of freezing stations:	2	2
Max. temperature difference:	17 K, at chamber temp. of -35°C	17 K, at chamber temp. of -35°C
Specimen cooling
Temperature range	-10 to -50 °C ± 3 K	-10 to -50 °C ± 3 K
Refrigeration capacity	320 W	320 W
Cut-out pressure	25 bar	25 bar
Safety factor	3	3
Refrigerant and quantity	at 230V/50 Hz 140 g (± 5 g), refrigerant R-404A*	at 120V/60 Hz 110 g (± 5 g), refrigerant R-404A*
	at 100V/50/60 Hz 110 g (± 5 g), refrigerant R-404A*
Compressor oil*	0.4 l alpha 22, Kyodo*	0.4 l alpha 22, Kyodo*
Defrosting of specimen head
Automatic defrost:	No	No
Manual defrost
Defrost time	15 min.	15 min.

*) Refrigerant and compressor oil must be replaced by qualified, authorized service personnel only.

acc. to CECOMAF: liquid temperature 45 °C, evaporation temperature: -25 °C

Leica CM1950 – Cryostat

15

4.Standard Delivery

Basic instrument WITHOUT motor/WITHOUT extraction, in the specific voltage variant

1 handwheel, manual .........................................................................................	0477 41346
5 specimen discs, 30mm ...................................................................................	0477 40044
1 section waste tray ...........................................................................................	0477 40062
1 position holder for freeze shelf .....................................................................	0477 40080
1 freeze shelf cover ............................................................................................	0477 43763
1 tool set ...............................................................................................................	0436 43463
- 1 brush, fine ................................................................................................	0183 28642
- 1 Leica brush w/magnet ...........................................................................	0183 40426
- 1 Allen key, No. 1.5 .....................................................................................	0222 10050
- 1 Allen key, No. 2.5 .....................................................................................	0222 04137
- 1 Allen key, No. 3.0 .....................................................................................	0222 04138
- 1 Allen key, No. 4.0 .....................................................................................	0222 04139
- 1 Allen key with dome head, No. 4.0.......................................................	0222 32131
- 1 Allen key, No. 5.0 .....................................................................................	0222 04140
- 1 key with handle, No. 5.0 .........................................................................	0194 04760
- 1 Allen key, No. 6.0 .....................................................................................	0222 04141
- 1 Single-head wrench, No. 13 .................................................................	0330 33149
- 1 Single-head wrench, No. 16 .................................................................	0330 18595
1 power cable .........................................................................................................................
1 bottle of cryostat oil, type 407, 50 ml ............................................................	0336 06098
1 bottle of OCT freezing compound, 125 ml ....................................................	0201 08926
1 pair of safety gloves, size M, * for cryosectioning ....................................	0340 29011
1 user manual plus language CD .....................................................................	0708 37115

*Note: for the Japanese version: 100V, 50/60 Hz; 1 pair of safety gloves, size S (0340 40859) is included.

Basic instrument WITHOUT motor and WITH extraction,
Standard scope of delivery as above,
plus:
1 accessory set (extraction) .............................................................................	0477 43300
- Hose adapter 1 ...........................................................................................	0477 40293
- Hose adapter 2 ...........................................................................................	0477 40294
- Suction nozzle ............................................................................................	0477 40295
- Silicone hose ..............................................................................................	0477 43302
- Silicone stopper .........................................................................................	0477 43304
- Chamber suction nozzle ...........................................................................	0477 43779
- Set of filters (5 pieces) ..............................................................................	0477 43792

Compare the delivered components with the parts list and your order. Should you find any discrepancies, please contact your Leica sales office without delay.

A choice of different knife holders is available for the CM1950.

16	Operating Manual V1.1 – 05/2007

4. Standard Delivery

Basic instrument WITH motor/WITHOUT extraction, in the specific voltage variant

1 handwheel, motorized ....................................................................................	0477 41347
5 specimen discs, 30mm ...................................................................................	0477 40044
1 section waste tray ...........................................................................................	0477 40062
1 position holder for freeze shelf .....................................................................	0477 40080
1 freeze shelf cover ............................................................................................	0477 43763
1 tool set ...............................................................................................................	0436 43463
- 1 brush, fine ................................................................................................	0183 28642
- 1 Leica brush w/magnet ...........................................................................	0183 40426
- 1 Allen key, No. 1.5 .....................................................................................	0222 10050
- 1 Allen key, No. 2.5 .....................................................................................	0222 04137
- 1 Allen key, No. 3.0 .....................................................................................	0222 04138
- 1 Allen key, No. 4.0 .....................................................................................	0222 04139
- 1 Allen key with dome head, No. 4.0.......................................................	0222 32131
- 1 Allen key, No. 5.0 .....................................................................................	0222 04140
- 1 key with handle, No. 5.0 .........................................................................	0194 04760
- 1 Allen key, No. 6.0 .....................................................................................	0222 04141
- 1 Single-head wrench, No. 13 .................................................................	0330 33149
- 1 Single-head wrench, No. 16 .................................................................	0330 18595
1 power cable .........................................................................................................................
1 bottle of cryostat oil, type 407, 50 ml ............................................................	0336 06098
1 footswitch dummy ...........................................................................................	0443 30420
1 bottle of OCT freezing compound, 125 ml ....................................................	0201 08926
1 pair of safety gloves, size M, * for cryosectioning ....................................	0340 29011
1 operating instructions plus language CD....................................................	0708 37115

*Note: for the Japanese version: 100V, 50/60 Hz; 1 pair of safety gloves, size S (0340 40859) is included.

Basic instrument WITH and WITH extraction, in the specific voltage variant
Standard scope of delivery as above,
plus:
1 accessory set (extraction) .............................................................................	0477 43300
- Hose adapter 1 ...........................................................................................	0477 40293
- Hose adapter 2 ...........................................................................................	0477 40294
- Suction nozzle ............................................................................................	0477 40295
- Silicone hose ..............................................................................................	0477 43302
- Silicone stopper .........................................................................................	0477 43304
- Chamber suction nozzle ...........................................................................	0477 43779
- Set of filters (5 pieces) ..............................................................................	0477 43792

Compare the delivered components with the parts list and your order. Should you find any discrepancies, please contact your Leica sales office without delay.

A choice of different knife holders is available for the CM1950.

Leica CM1950 – Cryostat

17

5.General Overview

Cryostat chamber (with activated UV disinfection)

Bacterial filter (optional, only for instruments with filter)

	Emergency-stop	On/off switch, also circuit
	switch (motorized
		breaker
	instruments only)

Footswitch dummy (motorized instruments only)

Handwheel in 12 o’clock position

Unscrew and align adjusting feet after transport

Catch tank for condensate

Fig. 10

Castors for safe transport

over short distances

Align adjusting feet for secure upright position

18	Operating Manual V1.1 – 05/2007

5. General Overview

5.1Control Panel Fields and Cryostat Chamber

1 - Control panel field 1: Extraction, temperature and time control, illumination, UV disinfection 2 - Control panel field 2: Electric coarse feed (sectioning and trimming thickness adjustment)

3 - Controlpanelfield3:Motorizedsectioningcutting,optional(adjustmentofstroketype,cuttingspeedetc.)

1

		7
4	6			15	16
		10a
			10	10c		14
	5
		10b	11
2					3
					12

9	13
8

			Fig. 11
4	- Heat conductor block, stationary (optional)	10c-Knife guard on the blade holder CE
5	- Peltier element (with 2 stations)	11	- Extraction nozzle on the extraction hose
6	- Freeze shelf, 15 positions	12	- Extraction hose for section waste
7	- Position holder on freeze shelf	13	- Brush shelf (optional)
8	- Heat and cold extractor, mobile (opt.)	14	- Adapter piece for extraction hose
9	- Shelf, movable (optional)		(the coarse filter insert is behind it)
10	- Blade holder CE with blade ejector (a)	15	- Object head, directional
10bFinger rest on the blade holder CE		16	- Waste tray

Leica CM1950 – Cryostat

19

6.Installation

6.1Site Requirements

The place of installation must meet the following requirements:

•No direct sunlight.

•Power supply at a distance no greater than approx. 3 m.

•No drafts (air condition outlets etc.).

•Even floor.

•Vibration-free floor.

•The instrument is designed for indoor use only.

•Obstruction-free access to the handwheel.

•The power switch/circuit breaker must be freely and easily accessible.

•Room temperature always approx. 22°C.

•The relative humidity must not exceed 60%.

To ensure proper function of the instrument, it must be set up while maintaining a minimum distance from walls and furniture.

• Distance to walls and furniture, calculated from the cabinet:

- rear:	15 cm
- right side:	30 cm
- left side:	15 cm

• No heat dissipating appliances around.

High room temperatures and excessive air humidity affect the cooling capacity of the cryostat and lead to ice forming in the instrument!

• Plug the instrument into power sockets with ground only. Power cable length: up to 3.5 m

Extendable: no

20	Operating Manual V1.1 – 05/2007

6. Installation

6.2Transport to the Site

•First, check if the location meets the conditions specified in "Site requirements".

•Transport the instrument to the desired location.

•Observe the following:

The instrument must be transported in an upright position or slightly tilted (max. 30°)!

When tilting the instrument 2 people must counterbalance from the front side to prevent the instrument from falling down and causing severe injury!

	• When transporting the instrument on wheels
	(2) grip the cabinet only at the marked loca-
	tions (		).
	• To do so, unscrew the adjustable feet using
	the No. 13 open-end wrench (when subse-
	quently transporting the instrument on cas-
	tors, screw the feet back in as far as they will
	go). To ensure a secure upright position at its
	intended location, align both adjusting feet (1).
			Before transport or relocation, remove
			the filter bag from the chamber. If you
			fail to do this, the filter bag will thaw,
			then freeze solid when the instrument
			is reconnected. When subsequently
			removed, the filter will be destroyed,
	2		causing section waste to get into the
1			bacteria filter. (Refer to p. 26.)

Fig. 12

2When not using the extraction for a long

	1	period, tightly close the opening for the
		extraction hose using the silicone stop-
	2 (covered)	per (5, page 28) included in the scope of
		delivery.

Leica CM1950 – Cryostat

21

6.Installation

Transport with a fork lift

• The instrument can be transported with a fork lift.

To ensure a safe transport with a fork lift 3 people are required: one operating the fork lift, and the other 2 holding the instrument on either side to prevent it from sliding down.

•At the installation site, unscrew the adjustable feet (1) with the openend wrench No. 13 (see Fig. 12). This is absolutely necessary for a stable stand.

Total width with handwheel

Fig. 13

6.3 Assembling the Handwheel

			Rotate the handwheel only if the
			refrigeration system is on and the
			cryochamber is cold.
	4	• Insert the pin (1) of the handwheel shaft into
		the hole (2) of the handwheel.
	2
	1	• Tighten the screw (4) using the No. 6 Allen key.
		• Place the protective cap on the screw (4).
		To dismount, proceed in reverse order.
	Fig. 14

22	Operating Manual V1.1 – 05/2007

6. Installation

6.3.1 Locking/Unlocking the Handwheel

12 o’clock position

6 o’clock position

Fig. 15

1

2

Fig. 16

Only rotate the handwheel when the refrigeration system is on and the cryochamber is cold!

Always lock the handwheel prior to manipulating the knife or specimen, changing the specimen, or taking a break.

To lock the handwheel, move its handle to the 12 or 6 o’clock position. Press the lever (1) fully outward; gently rock the handwheel until the locking mechanism clicks into place noticeably.

To release the handwheel, press the lever (2) on the handwheel toward the cryostat housing.

Centering the Handwheel (Optional)

An important safety device on the cryostat is the feature for centering the handwheel in motorized sectioning mode.

To do so, pull out the handwheel’s handle and position it in the middle of the handwheel. The handle automatically engages in this position.

Fig. 17

6.3.2 Installing the Footswitch Dummy (Instruments with Cutting Motor)

•The footswitch dummy must be installed on the outer right side of the instrument (see page 16, General overview) if no footswitch (optional) is used.

If the red LED in the E-STOP field in control panel field 3 is illuminated, either:

•emergency-stop function active, or

•footswitch dummy (optional footswitch) connected incorrectly or not at all.

Fig. 18

Leica CM1950 Cryostat

23