Trans-Blot® Turbo™ Blotting System
Instruction Manual
Catalog Number 170-4155
Bio-Rad Technical Support
For help and technical advice, please contact the Bio-Rad Technical Support department. In the United States, the Technical Support department is open Monday–Friday, 5:00 am–5:00 pm, Pacific Time.
Phone: 1-800-424-6723
Fax: 1-510-741-5802
Email: LSG_TechServ_US@bio-rad.com (for U.S. and international customers)
Online technical support and worldwide contact information are available at www.consult.bio-rad.com.
Legal Notices
No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage or retrieval system, without permission in writing from Bio-Rad Laboratories.
Bio-Rad reserves the right to modify its products and services at any time. This user guide is subject to change without notice.
Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors, or for any damage resulting from the application or use of this information.
Tween is a trademark of ICI Americas, Inc.
Copyright © 2010 by Bio-Rad Laboratories. All rights reserved.
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Trans-Blot® Turbo™ Blotting System 
Safety Warning Labels
Warning: Do not open or attempt to repair the Trans-Blot® Turbo™ instrument or cassettes. Doing so will void your warranties and can put you at risk for electric shock. Return the Trans-Blot Turbo instrument or cassettes to the factory (US customers) or to an authorized distributor (all other customers) if repairs are needed.
Under normal operating conditions, Trans-Blot Turbo cassettes may become warm during transfer.
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Trans-Blot® Turbo™ Blotting System 
Safety Compliance
This instrument has been tested and found to be in compliance with all applicable requirements of the following safety and electromagnetic standards:
•EN61010-1 Electrical Equipment for Measurement, Control, and Laboratory Use
•UL STD No. 61010A-1 Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements
•CAN/CSA C22.2 No. 61010-1-04 Safety Requirements for Measurement, Control, and Laboratory Use, Part 1: General Requirements (includes Amendment 1)
•IEC 61010-1 Safety Requirements for Measurement, Control, and Laboratory Use, Part 1 General Requirements
Electromagnetic Compatibility (EMC)
EN61326 Class A Electrical Equipment for Measurement, Control, and Laboratory Use, General Requirements
FCC Warning and Notes
Warning: Changes or modification to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
This Bio-Rad instrument is designed and certified to meet EN61010* and the EMC requirements of EN61326 (for class A) safety standards. Certified products are safe to use when operated in accordance with the instruction manual. This instrument should not be modfied or altered in any way.
Alteration of this instrument will:
•Void the manufacturer’s warranty
•Void the EN61010 safety certification
•Create a potential safety hazard
* EN 61010 is an internationally accepted electrical safety standard for laboratory instruments.
Bio-Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent.
We strongly recommend that you follow the safety specifications listed in this section and throughout this manual. Use only the supplied power cord with the instrument, making sure to choose the plug adaptor that corresponds to the electrical outlets in your region.
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Trans-Blot® Turbo™ Blotting System 
Specifications
Trans-Blot® Turbo™ Instrument
Dimensions: (L x W x H) 26.0 x 21.1 x 20.4 cm Weight: 8 lb with cassettes, 4.5 lb without cassettes
Input power: 100–240 VAC, 276 VA, 50–60 Hz, 175 W max
Fuses: Two 6.3 A, 250 V, fuses located above the power connection On/off switch: Yes, mains connected
USB port: Yes, input only, for firmware updates Cooling fan: Yes
Output power: 0–26 VAC (1 V increments), 0–2.6 A DC (0.1 A increments) for each cassette Operating conditions: 15–31°C ambient temperature, 0–95% relative humidity (noncondensing)
User Interface
18 button keypad, 128 x 64 pixel monochrome display Programmable methods: Up to 25 user defined
Preprogrammed methods: Standard SD, 1.5 mm gels, High MW, Low MW, Mixed MW, 1 Mini-PROTEAN®
TGX™ gel
Audible alarm: Yes User notifications:
•Power fail during run
•No load detection
•No cassette detection
•End of run
•Watt/hr limit
Trans-Blot Turbo Cassettes
Dimensions: (L x W x H) 20.2 x 16.0 x 4.5 cm
Anode: Platinized titanium electrode plate
Cathode: Stainless steel
Weight: 1.5 lb
Gel Compatibility
Suitable for transfer of two mini format gels or one midi fomat gel per cassette. Other gel sizes can be trimmed to fit the consumable transfer packs.
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Trans-Blot Transfer Packs
Tray dimensions (L x W): 18.0 x 14.5 cm
Stack dimensions (L x W): Mini format (7.0 x 8.5 cm), midi format (13.5 x 8.5 cm), + tab Pad materials and buffer: proprietary
Membrane: 0.2 µm nitrocellulose or 0.2 µm PVDF
Note: Tray base is polyethylene terephthalate glycol (PETG) and can be recycled according to local regulations for class 1 plastics.
Trans-Blot Turbo RTA Transfer Kits
Membrane dimensions (L x W): Mini format (7.0 x 8.5 cm), midi format (13.5 x 8.5 cm) Membrane: 0.2 µm nitrocellulose, 0.2 µm PVDF, or 0.45 µm low fluorescent PVDF Pad materials and buffer: proprietary
Gel tray: two reusable trays to wet and equilibrate membrane and pads
Table of Contents
Bio-Rad Technical Support |
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Safety Warning Labels |
iii |
Safety Compliance |
v |
Electromagnetic Compatibility |
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FCC Warnings and Notes |
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Specifications |
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Section 1: Introduction |
1 |
Unpacking and Setup Instructions |
2 |
Chemical Compatibility |
2 |
Safety/Cautions/Warning |
2 |
Warranty |
3 |
Section 2: Equipment and Reagents Overview |
4 |
2.1 User Interface |
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Section 3: Trans-Blot® Turbo™ System Transfer Setup |
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3.1 Transfer Using Transfer Packs |
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3.2 Placing a Transfer Pack into the Cassette |
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3.3 Transfer Using RTA Transfer kits |
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3.4 Transfer Using the Turbo Protocol |
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3.4.a. Disassembling and Removing the Membrane |
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3.5 Transfer Using Other Preprogrammed Protocols |
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3.6 Transfer Using a New Protocol |
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3.7 Transfer Using Traditional Semi-Dry Consumables |
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3.8 Optimizing Transfer Conditions |
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Section 4: Appendices |
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Appendix A. Maintenance |
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Appendix B. Troubleshooting |
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Overview of the Three Protocol Modes |
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TURBO |
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LIST |
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NEW |
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Consumables and Related Products |
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