AMPLIFICATION
CFX Manager™ Software
Protocol Quick Guide
Experiment Setup Protocol Tab
The Protocol tab displays a preview of the protocol loaded in the Experiment Setup window (Figure 1).
Click Create New to open the Protocol Editor to create a new protocol.
Click Select Existing to launch the file browser
to load a protocol to use in an experiment or to edit.
Use the Express Load drop-down menu to directly load a protocol to use in an experiment or to edit.
Click Edit Selected to open the Protocol Editor to edit the steps of the selected protocol.
Click the Start Run tab to proceed and run an experiment with the currently loaded protocol.
Protocol Editor
The Protocol Editor is used to create a new protocol or edit an existing one (Figure 2).
1.Select any step in either the graphical or text display — the step becomes highlighted in blue. Click on the temperature or dwell time to directly edit the value.
2.Click Insert Step to add a temperature step to the protocol.
Click Delete Step to remove a highlighted step from the protocol.
3.Click Add Plate Read to Step to designate when fluorescence data will be acquired during the protocol. The text on this button changes to Remove Plate Read if the currently highlighted step has a plate read. If you do not want to acquire data at that step, then click Remove Plate Read.
4.Click on the number of repeats of a GOTO Step to change the number of cycles in the protocol (Figure 2, Step 4).
Click on the GOTO Step number to change the steps included in the GOTO loop.
Fig. 1. Protocol tab in the Experiment Setup window. Load an existing protocol or create a new protocol for an experiment.
Graphical display
Text display
Protocol editing controls
Fig. 2. Protocol Editor. The protocol editing controls are on the left. Both the graphical and text displays can be edited. Step 1 is highlighted and 95˚C is selected for editing in the text display.
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