Instruction Manual
Precision Plus Protein™
Standard Plugs, Unstained
Catalog #161-0378
Product shipped at room temperature. Store at 4ºC upon arrival.
Products for 2-D Electrophoresis, Proteomics
Catalog # |
Description |
161-1511 |
PROTEAN Plus Tris-HCl Gel, 12%, 24 cm IPG well |
161-0787 |
Bio-Safe Coomassie Stain, 5 L |
161-0449 |
Silver Stain Plus Kit |
161-3138 |
SYPRO Ruby Protein Gel Stain, 5 L |
161-0737 |
Laemmli Sample Buffer, 30 ml |
161-0772 |
10 x Tris/Glycine/SDS, 5 L cube |
163-2092 |
PROTEAN Plus Overlay Agarose |
170-3990 |
Trans-Blot Plus Cell With Plate Electrodes and |
|
Super Cooling Coil |
162-0251 |
Nitrocellulose Membrane, 0.45 μm, 26.5 x 28 cm, 10 sheets |
162-0252 |
Nitrocellulose Membrane, 0.2 μm, 26.5 x 28 cm, 10 sheets |
162-0253 |
Supported Nitrocellulose Membrane, 0.45 μm, |
|
26.5 x 28 cm, 10 sheets |
162-0254 |
Supported Nitrocellulose Membrane, 0.2 μm, |
|
26.5 x 28 cm, 10 sheets |
162-0255 |
Immun-Blot PVDF Membrane, 25 x 28 cm, 10 sheets |
162-0256 |
Sequi-Blot PVDF Membrane, 25 x 28 cm, 10 sheets |
Products for Blot Detection
161-0380 StrepTactin-HRP Conjugate, 0.3 ml, 150 applications 161-0382 StrepTactin-AP Conjugate, 0.3 ml, 150 applications 170-5040 Immun-Star HRP Chemiluminescent Substrate, 500 ml 170-5012 Immun-Star AP Substrate Pack
This product is sold under license from Life Technologies Corporation, Carlsbad, CA, for use only by the buyer of the product. The buyer is not authorized to sell or resell this product or its components.
Bio-Rad Laboratories, Inc.
2000 Alfred Nobel Dr., Hercules, CA 94547
(510) 741-1000 |
4117684 Rev C |
Representative lot of Precision Plus Protein standard plug
on a 4–20% Tris-HCl SDS-PAGE gel
250 kD
150
100
75
50
37
25
20
15
10
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Precision Plus Protein Unstained Standard Plugs allow you to easily, quickly, and cleanly load molecular weight standards on any gel. The newest addition to the family of Precision Plus Protein standards, these ready-to-use standard plugs provide a convenient and reliable solution to molecular weight determination on 2-D electrophoresis gels. Specifically designed for use with PROTEAN Plus precast gels and other gels without a
separate standards well, Precision Plus Protein unstained standards are cast in 1 mm thick agarose plugs for easy storage, handling, and loading. Load concentrations have been optimized for SYPRO, Ruby, Silver Stain Plus, and Bio-Safe Coomassie staining. Precision Plus Protein standard plugs come in an easy to use, snap-off plastic plug casing, in packs of 2 x 12 (24 applications), with one application per plug.
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Product Description
Precision Plus Protein standard plugs contain ten recombinant protein bands of 10 kD, 15 kD, 20 kD, 25 kD, 37 kD, 50 kD, 75 kD, 100 kD, 150 kD, and 250 kD. The 25 kD, 50 kD, and 75 kD bands are three times the intensity of the other bands and serve as reference markers.
The molecular weights of Precision Plus Protein standard plugs, are confirmed by mass spectrometry and migration in a Laemmli SDS-PAGE system.
Precision Plus Protein standard plugs contain an integral Strep-tag sequence, enabling parallel detection on western blots using StrepTactinHRP (catalog #161-0380) or StrepTactin-AP (catalog #161-0382).
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