Bio-Rad Bio-Beads SM-2 Adsorbents User Manual

Bio-Beads®	Hydrophobic
and	Interaction

Adsorbents

Manual

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547

LIT195 Rev B

	Table of Contents
Section 1	Introduction ..........................................	1
Section 2	Product Description..............................	2
Section 3 Instructions for Use ..............................		4
3.1 Preparation of Bio-Beads SM
	Adsorbent (Bulk) ............................................	4
3.2	Column Method ..............................................	4
Section 4	Sample Protocols...................................	7
4.1 Triton X-100 Detergent Removal ...................		7
4.2	Adsorption of Unconjugated
	Fuorescent Dye ...............................................	8
4.3	Batch Protocol.................................................	8
4.4	Column Protocol .............................................	9
Section 5	Storage .................................................	10
Section 6	References............................................	10
Section 7	Assistance ............................................	12
Section 8	Product Information...........................	13

Section 1

Introduction

Bio-Beads SM adsorbents, for adsorbing organics from aqueous solutions, are neutral, macroporous polymeric Analytical Grade adsorbents of high surface area, which are intended for laboratory use only. The adsorbent is composed of a large number of highly crosslinked microspheres. This macroreticular structure gives it high surface area and uniform pore sizes. BioBeads adsorbents can be used with a variety of solvents, including alcohols, petroleum ether, diethyl ether, hexane, and solvent mixtures, as well as with aqueous media. They have excellent physical stability and will withstand temperatures up to 250 °C and pressures to 1,200 psi. Biotechnology Grade Bio-Beads SM adsorbents are specifically certified to contain less than 100 microorganisms per gram.

All Bio-Beads SM adsorbents are available in bulk packages.

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