Loading...
3.0
NGC™ Chromatography Systems
and ChromLab™ Software
Installation Guide
Version 3.0
NGC™ Chromatography
Systems and
ChromLab™ Software
Installation Guide
Version 3.0
Bio-Rad Technical Support Department
The Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific Time. Worldwide technical support is available on the Web at www.consult.bio-rad.com.
Phone: 1-800-424-6723, option 2
Fax: 1-510-741-5802
Email: LSG.TechServ.US@Bio-Rad.com (U.S.)
LSG.TechServ.Intl@Bio-Rad.com (International)
Web: www.consult.bio-rad.com
Notice
No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage or retrieval system, without permission in writing from Bio-Rad.
Bio-Rad reserves the right to modify its products and services at any time. This instrument guide is subject to change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors or omissions, or for any damage resulting from the application or use of this information.
PEEK is a trademark of Victrex plc.
Microsoft and Windows are trademarks of Microsoft Corporation.
Copyright © 2014 by Bio-Rad Laboratories. All rights reserved.
Table of Contents
Chapter 1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Main Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Safety Alerts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Regulatory Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Safety Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Electromagnetic Compatibility (EMC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
FCC Warning and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Finding Out More . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Chapter 2 Preparing Your Site . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Delivery Crate Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Room Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Bench Space. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Power Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
ChromLab System Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Preparing the ChromLab Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Chapter 3 Setting Up NGC Chromatography Systems . . . . . . . . . . . .21
NGC Systems Accessories Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
NGC Scout Accessories Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
NGC Discover Accessories Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
NGC Discover Pro Accessories Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Installation Guide |
iii |
Table of Contents
Attaching the Touch Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 NGC System Location Is on a Lab Bench. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 NGC System Location Is a Standard Cold Cabinet . . . . . . . . . . . . . . . . . . . . . 34
Connecting the Drain Tube to the Buffer Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Installing the pH Probe in the pH Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Attaching an Expansion Tier to the NGC Instrument . . . . . . . . . . . . . . . . . . . . . . 44 Inserting Additional Modules. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Connecting Peripheral Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Connecting the BioFrac Fraction Collector . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Connecting Column Clamps to the NGC Instrument . . . . . . . . . . . . . . . . . . . . 59 Connecting Tubing to the Columns. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 Connecting a Keyboard or Mouse. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Connecting the Air Sensors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 Connecting External Devices to the NGC Instrument . . . . . . . . . . . . . . . . . . . . 66
Connecting the Power and Communication Cables . . . . . . . . . . . . . . . . . . . . . . . 76 Connecting the NGC System to the ChromLab Computer. . . . . . . . . . . . . . . . . . 78 Establishing a Direct Cable Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 Establishing a Network Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Chapter 4 Installing ChromLab Software . . . . . . . . . . . . . . . . . . . . . . 81
Installing ChromLab Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Establishing the Connection Between ChromLab Software and
the NGC Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Powering on the NGC Instrument and Peripherals . . . . . . . . . . . . . . . . . . . . . . 84
Setting the System Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Setting Delay Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Setting Up Remote Access Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Chapter 5 Plumbing and Priming
the NGC Chromatography Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Preparing the Buffer and Waste Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Buffer Bottles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Waste Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
iv NGC Chromatography Systems and ChromLab Software
Table of Contents
Plumbing NGC Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
General Guidelines for Tubing Sizes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Plumbing All NGC Systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Plumbing the NGC Quest System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Plumbing the NGC Scout System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Plumbing the NGC Discover System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Plumbing the NGC Discover Pro System . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Priming and Purging the System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Priming and Purging the NGC Quest System . . . . . . . . . . . . . . . . . . . . . . . . . 124
Priming and Purging the NGC Scout System . . . . . . . . . . . . . . . . . . . . . . . . . 127
Priming and Purging the NGC Discover System. . . . . . . . . . . . . . . . . . . . . . . 131
Calibrating the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Calibrating pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Calibrating the Pump Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Calibrating Pressure Sensor Zero Point . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
Calibrating the Conductivity Monitor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
Appendix A Upgrading ChromLab Software and |
|
NGC Systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
147 |
Upgrading ChromLab Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
. 147 |
Updating NGC Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
. 148 |
Appendix B Troubleshooting ChromLab Software |
|
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
151 |
Possible Causes for Connection Failures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 Configuring the Network Adapter for Direct Connection . . . . . . . . . . . . . . . . . . 154 Troubleshooting Network Connection Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . 158 Locating the IP Address of the NGC Instrument. . . . . . . . . . . . . . . . . . . . . . . 158 Checking the Network Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 160 ChromLab Software Firewall Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162 Firmware Update Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
Installation Guide |
v |
Table of Contents |
|
Appendix C Connecting the C-96 Autosampler to |
|
NGC Systems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
165 |
Using the C-96 Autosampler with NGC Systems . . . . . . . . . . . . . . . . . . . . . . . . 165 Connecting the C-96 Autosampler to the NGC Instrument . . . . . . . . . . . . . . 165 Plumbing and Priming the C-96 Autosampler. . . . . . . . . . . . . . . . . . . . . . . . . 168 Setting Up the C-96 Autosampler for Use in ChromLab Methods . . . . . . . . . 172
Appendix D Firewall Configuration . . . . . . . . . . . . . . . . . . . . . . . . 173
Firewall Inbound Rules for Bio-Rad NGC Software . . . . . . . . . . . . . . . . . . . . . . 173
vi NGC Chromatography Systems and ChromLab Software
1 Introduction
The NGC™ chromatography systems are preparative systems designed to rapidly automate the purification of biomolecules. The flexible, modular, and economical design makes NGC the instrument of choice for method development and scale-up. It is available in six preplumbed, factory-tested configurations at two different flow rates. Each preconfigured system can be further customized and upgraded by adding valves, detectors, or pumps in order to meet specific application needs. Any system can be configured for either low flow rate or high flow rate operation by simply changing the pump head on the system pump modules. As a result, a single hardware platform can be modified as the application need and scale change.
ChromLab™ software enables you to control, manage, and evaluate the NGC system and the purification runs.
Installation Guide |
7 |
1Introduction
Main Features
NGC chromatography systems enable you to do the following:
Easily create purification and maintenance protocols from predefined method templates and protocol phases
Automate multicolumn purification processes using preprogrammed templates and multiple column switching valves
Automate multiple sample injections using either the sample inlet valve and the sample pump or the C-96 autosampler
Expand sample monitoring using the signal import module (SIM) to export digital signals to, and import digital signals from, external detectors
Collect large-volume fractions using multiple outlet valves while also collecting small-volume fractions using the BioFrac fraction collector
Automatically prepare buffers using preprogrammed buffer blending protocols
Analyze purification results through 1-click peak integration, determine protein concentration and calculate column performance
Automate purification protocol optimization using the scouting wizard
Easily locate fractions containing peaks of interest and view the protein concentration within each fraction
Extend the preconfigured systems with additional valves for buffers, samples, and columns
Organize the location of the modules to optimize separation performance based on method scale and complexity, and to minimize the system swept volume
Minimize errors when connecting tubing using the Point-to-Plumb™ feature in ChromLab software
8NGC Chromatography Systems and ChromLab Software
Safety Information
Safety Information
This section explains the different safety alerts within this document. This section also provides information about the safe use and setup of the instrument.
Safety Alerts
This document contains WARNINGS and Cautions pertaining to the installation and use of the NGC instrument.
Alert Icon |
Definition |
Flammable/ |
A hazardous situation that could result |
Extreme Heat |
in serious personal injury or extreme |
|
damage to the instrument. Do not |
|
proceed until all stated conditions are |
|
met and clearly understood. |
Electrical Shock |
A hazardous situation that could result |
|
in serious personal injury or extreme |
|
damage to the instrument. Do not |
|
proceed until all stated conditions are |
|
met and clearly understood. |
|
A hazardous situation that could result |
|
in minor or moderate personal injury or |
|
damage to the instrument. Do not |
|
proceed until all stated conditions are |
|
met and clearly understood. |
Safety Precautions
Caution: The weight of the NGC system varies from moderate to heavy depending on the system in place. Take care when lifting the instrument onto the lab bench or into a standard cold cabinet. To reduce the risk of personal injury or damage to the instrument, three or more people should perform this task.
WARNING! Disconnect power to the NGC system before inserting, removing, or moving modules. No user-serviceable parts are inside any component unless noted in this manual. Refer servicing questions to Bio-Rad service personnel.
Installation Guide |
9 |
1Introduction
Regulatory Information
The NGC instrument has been tested and found to be in compliance with all applicable requirements of the following safety and electromagnetic compliance standards. The NGC instrument is labeled with the following compliance marks.
Safety Compliance
CE Mark:
EN61010-1 Electrical Equipment for Measurement, Control, and Laboratory Use
IEC 61010-1 Safety Requirements for Measurement, Control, and Laboratory Use, Part 1: General Requirements
cTUVus Mark:
UL STD No. 61010-1 Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements
CAN/CSA C22.2 No. 61010-1-12 Safety Requirements for Measurement, Control, and Laboratory Use, Part 1: General Requirements (includes Amendment 1)
Products with these safety compliance marks are safe to use when operated in
accordance with the instruction manual. This does not extend to accessories with
no marks, even when used with this unit.
Electromagnetic Compatibility (EMC)
CE Mark:
EN61326 Class A Electrical Equipment for Measurement, Control, and Laboratory Use, General Requirements
The NGC instrument is designed to operate in a controlled electromagnetic
environment. Do not use radio transmitters such as mobile telephones within 10 ft
(3 m) of the instrument.
10 NGC Chromatography Systems and ChromLab Software
Regulatory Information
FCC Warning and Notes
The NGC instrument complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
It is possible that emissions from the NGC instrument may interfere with some sensitive appliances when placed nearby or on the same circuit as those appliances. The user should be aware of this potential and take appropriate measures to avoid interference.
The NGC instrument should not be modified or altered in any way. Alteration of the NGC instrument will:
Void the manufacturer’s warranty
Void the IEC/EN/UL/CSA 61010-1 safety compliance
Create a potential safety hazard
Bio-Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent.
Important: The NGC instrument is intended for laboratory use only.
We strongly recommend that you follow the safety specifications listed throughout this manual. Use only the supplied power cord with the instrument, making sure to choose the plug adaptor that corresponds to the electrical outlets in your region.
Installation Guide |
11 |
1Introduction
Finding Out More
After you install NGC documentation from the NGC Chromatography Systems Documentation DVD, you can access installed NGC guides and tutorials on the Help menu in any ChromLab view.
More information about the NGC chromatography systems and ChromLab software is available from the following sources.
The NGC Chromatography Systems and ChromLab Software Instrument Guide is available on your NGC Chromatography Systems Documentation DVD as a .pdf file. This illustrated guide details the modules that make up the NGC instrument and includes troubleshooting and maintenance information.
The NGC Chromatography Systems and ChromLab Software User Guide is available on your NGC Chromatography Systems Documentation DVD as a .pdf file. This guide explains how to use ChromLab software to set up and control the NGC instrument, run protein separations and other operations manually, program methods to automate purification runs, evaluate the results, and print experiment reports.
For ChromLab Help, clicking 
in dialog boxes accesses relative information. Screen-level help is also available on the Help menu.
NGC video tutorials are available on the NGC Chromatography Systems Documentation DVD as .mp4 files.
Tip: You can click the Bio-Rad logo in the upper-left corner of any ChromLab window to launch the Bio-Rad website.
12 NGC Chromatography Systems and ChromLab Software
2 Preparing Your Site
This chapter explains how to prepare your site to successfully install your NGC™ chromatography system and ChromLab™ software.
Delivery Crate Dimensions
The crated NGC instrument is very heavy, approximately 160–215 lb (72.6–97.6 kg) depending on the configuration. Bio-Rad strongly recommends that you use a pallet jack to move the instrument pallet from the shipping dock to the laboratory.
Table 1. Specifications for the NGC delivery crate
Item |
Specification |
|
|
NGC Quest™ |
Length: 32 in.; 81.3 cm |
|
Width: 27 in.; 68.6 cm |
|
Height: 33 in.; 83.8 cm |
|
Weight: 161 lb; 73.1 kg |
|
|
NGC Quest Plus |
Length: 32 in.; 8138 cm |
|
Width: 27 in.; 68.6 cm |
|
Height: 33 in.; 83.8 cm |
|
Weight: 167 lb; 75.8 kg |
|
|
NGC Scout™ |
Length: 32 in.; 81.3 cm |
|
Width: 27 in.; 68.6 cm |
|
Height: 33 in.; 83.8 cm |
|
Weight: 166 lb; 75.4 kg |
Installation Guide |
13 |
2Preparing Your Site
Table 1. Specifications for the NGC delivery crate, continued
Item |
Specification |
|
|
NGC Scout Plus |
Length: 32 in.; 81.3 cm |
|
Width: 27 in.; 68.6 cm |
|
Height: 33 in.; 83.8 cm |
|
Weight: 172 lb; 78.1 kg |
|
|
NGC Discover™ |
Length: 32 in.; 81.3 cm |
|
Width: 27 in.; 68.6 cm |
|
Height: 41 in.; 104.1 cm |
|
Weight: 216 lb; 98.1 kg |
|
|
NGC Discover Pro |
Length: 27 in.; 68.6 cm |
|
Width: 27 in.; 68.6 cm |
|
Height: 14 in.; 35.6 cm |
|
Weight: 40 lb; 18.2 kg |
Note: The fourth tier (the upper tier on the NGC Discovery Pro system) is packaged separately.
14 NGC Chromatography Systems and ChromLab Software
Room Requirements
Room Requirements
Bench Space
Ensure that the bench or rack on which you install the NGC system is designed to support its weight.
Table 2. Specifications for the NGC instrument
Item |
Specification |
|
|
NGC instrument dimensions |
Length: 24 in.; 61.0 cm |
|
Width: 19 in.; 48.3 cm |
|
Height (measured from lab bench): |
|
NGC Quest and NGC Scout series: |
|
19 in.; 48.3 cm |
|
NGC Discover series: |
|
26.5 in.; 67.3 cm |
|
NGC Discover Pro series: |
|
34 in; 86.4 cm |
Note: These dimensions are for the NGC instrument only. They do not include the touch screen or accessories such as columns, air detectors, and buffer tray.
NGC instrument weight |
NGC Quest: 90 lb; 40.9 kg |
|
NGC Quest Plus: 97 lb; 44.0 kg |
|
NGC Scout: 94 lb; 42.7 kg |
|
NGC Scout Plus: 100 lb; 45.4 kg |
|
NGC Discover: 141 lb; 64 kg |
|
NGC Discover Pro: 171 lb; 77.6 kg |
|
|
Instrument top with buffer tray |
Height: 3 in.; 7.6 cm |
|
Weight: 8 lb; 3.6 kg |
Installation Guide |
15 |
2Preparing Your Site
Table 2. Specifications for the NGC instrument, continued
Item |
Specification |
|
|
Touch screen dimensions |
Height: 10 in.; 25.4 cm |
|
Length: |
|
Arm contracted: 13 in.; 33.0 cm |
|
Arm extended: 15 in.; 38.1 cm |
|
Width: |
|
Arm contracted: 1 in.; 2.5 cm |
|
Arm extended: 4 in.; 10.2 cm |
|
|
Touch screen weight (with arm) |
4 lb; 1.8 kg |
|
|
Environment
Table 3. Supported environmental specifications
Parameter |
Temperature Range |
Humidity Range |
|
|
|
Operating conditions |
4 to 40°C |
0–95% RH, |
|
(35 to 104°F) |
noncondensing |
|
|
|
|
|
|
Storage conditions |
4 to 40°C |
0–95% RH, |
|
(35 to 104°F) |
noncondensing |
|
|
|
|
|
|
Shipping conditions |
–40 to 65°C |
0–95% RH, |
|
(–40 to 149°F) |
noncondensing |
|
|
|
|
|
|
16 NGC Chromatography Systems and ChromLab Software
Power Requirements
Power Requirements
The power supply to the NGC system must be stable and within specifications at all times to ensure optimal operation. The power cable connected to the power inlet port must be rated for 7 amps or more.
Table 4. Power requirements for the NGC instrument and the computer running ChromLab
Parameter |
Requirement |
|
|
Mains input voltage |
AC 100–240V; 50–60 Hz |
|
|
NGC maximum power usage |
<750 watts |
|
|
Number of power sockets |
Minimum of three power sockets: |
|
One socket for the NGC instrument |
|
One socket for the computer running |
|
ChromLab software |
|
One socket for the BioFrac™ fraction collector |
|
A socket for any supported peripheral |
|
instrument that you attach (for example, the |
|
C-96 autosampler) |
|
Tip: The touch screen does not require a |
|
separate power supply. |
|
|
Type of power sockets |
IEC type connections, grounded sockets |
|
|
Installation Guide |
17 |
2Preparing Your Site
ChromLab System Requirements
Bio-Rad can provide a PC with ChromLab software preinstalled or you can install ChromLab onto a computer at your site. Table 5 lists the minium system requirements for the computer running ChromLab (known as the ChromLab computer).
Table 5. Minimum hardware requirements for the ChromLab computer
Hardware |
Minimum requirement |
|
|
Computer |
|
|
|
Operating system |
Either: |
|
Microsoft Windows 7 SP1 (32-bit) |
|
Microsoft Windows 7 SP1 (64-bit) |
|
Microsoft Windows 8 |
|
|
Processor |
Intel dual-core or equivalent, 2.0 GHz |
|
|
RAM |
4 GB |
|
|
Hard disk space |
160 GB |
|
|
Required peripherals: |
DVD drive |
|
USB 2.0 high speed ports (2 minimum) |
|
Mouse |
|
Keyboard |
|
1 GB ethernet port (1) |
|
|
Monitor |
|
|
|
Screen resolution |
1200 x 900 (1280 x 1024 recommended) |
|
|
Screen colors |
True color mode (24 bits) or higher |
|
|
Screen size |
20" or higher |
|
|
18 NGC Chromatography Systems and ChromLab Software
Preparing the ChromLab Computer
Preparing the ChromLab Computer
To ensure the ChromLab computer operates the NGC system smoothly and accurately displays ChromLab software you might need to
Turn off sleep/hibernate mode
Turn off automatic updates
Set the display fonts
To turn off sleep/hibernate mode
1.On the ChromLab computer, select Control Panel > Power Options and click Change plan settings for the selected power plan.
2.On the Change settings for the plan page, click Change advanced power settings.
3.On the Advanced settings tab, expand the Sleep option, then expand Sleep after and do one of the following:
If your ChromLab computer is a laptop, click On battery or Plugged in (or both) and select Never from the dropdown list.
If your ChromLab computer is a desktop computer, click Setting and select Never from the dropdown list.
4.On the Advanced settings tab, expand Hibernate after, and then do one of the following:
If your ChromLab computer is a laptop, click On battery or Plugged in (or both) and select Never from the dropdown list.
If your ChromLab computer is a desktop computer, click Setting and select Never from the dropdown list.
5.Click OK to save the settings and close the Power Options dialog box.
Installation Guide |
19 |
2Preparing Your Site
To turn off automatic updates
1.On the ChromLab computer, select Control Panel > Windows Update and choose Change settings in the left pane.
2.Under Important updates, choose Never check for updates (not recommended) from the dropdown list.
3.Click OK to save the setting and close the Change Settings window.
To change the font size
1.On the ChromLab computer, select Control Panel > Fonts and choose Change font size in the left pane.
2.Select Smaller – 100% (default) if it is not already selected and click Apply.
3.Close the Display window.
20 NGC Chromatography Systems and ChromLab Software
3 Setting Up NGC
Chromatography Systems
After you unpack the NGC™ chromatography system and install it in a standard cold cabinet or on the lab bench you will need to
Attach the touch screen
Connect the drain tube to the buffer tray
Connect the power and communication cables
Connect the NGC system to the ChromLab™ computer
This chapter explains how to perform these and other setup tasks.
Tip: Before setting up the system, familiarize yourself with the NGC system and its modules, ports, accessory kits, cables, and power switches.
Installation Guide |
21 |
3Setting Up NGC Chromatography Systems
NGC Systems Accessories Kit
Each NGC system ships with an accessories kit. The contents of the kit are specific to the system. Verify the contents against those listed in the sections that follow.
The accessories kits contain the tubing, loops, and fittings necessary to complete the setup of the shipped configuration. All kits include
NGC fittings kit
1 ml syringe
Luer adaptor
Fittings tightener
Touch screen and pivot arm kit
Tool to attach touch screen
Pump head wash inlet tubing (2)
Pump head wash priming line
1/4-28 union
System pump inlet tubing (2)
1 ml sample loop
Waste tubing (2)
Injection port
40 psi backpressure regulator
Tubing retainers, small (1, for PEEK tubing management)
Tubing retainers, large (2, for PTFE tubing management)
Column tubing (2) and fittings (from inject valve to column top and from column bottom to UV detector inlet)
Tube #2 (conductivity flow cell to 40 psi backpressure regulator)
Tube #4 (backpressure regulator to the BioFrac™ fraction collector)
22 NGC Chromatography Systems and ChromLab Software
NGC Systems Accessories Kit
NGC Scout Accessories Kit
The kit for the NGC Scout™ system includes all the accessories in the NGC systems kit as well as
Tube #2 (40 psi backpressure regulator to pH valve inlet)
pH waste tubing
pH calibration port inlet tubing
Colored tubing for the buffer blending valve
NGC Discover Accessories Kit
The kit for the NGC Discover™ system includes all accessories in the NGC Scout kit as well as
Pump head wash inlet tubing (2) for the sample pump
Pump head wash priming line for the sample pump
1/4-28 union
Sample pump inlet tubing
System pump inlet tubing (14) for inlet valves
Column tubing (10) and fittings (from the column switching valve Top positions to column top, and from column bottom to column switching valve Bottom positions)
NGC Discover Pro Accessories Kit
The kit for the NGC Discover Pro system includes all the accessories in the NGC Discover kit as well as
Buffer inlet tubing (8) for inlet valve
Tube #6 for the inlet valve
Outlet valve tubing (12) for the outlet valve
Tube #10 for the outlet valve
Installation Guide |
23 |
3Setting Up NGC Chromatography Systems
Attaching the Touch Screen
The installation procedure for the touch screen varies depending on the location of the NGC instrument (lab bench or standard cold cabinet). This section explains how to attach the touch screen to the instrument in either location.
The communication cable for the touch screen is approximately 5' (1.52 m) in length. A 10' or 15' (3.0 or 4.57 m) cable is also available.
Important: The communication cable for the touch screen does not have a standard DVI connector, even though its connector resembles one. Do not attempt to connect the touch screen to the NGC system with any cable other than the one supplied with the system to avoid serious damage to the instrument.
NGC System Location Is on a Lab Bench
Note: This procedure assumes you will attach the touch screen on the left side of the NGC instrument. If you plan to attach the touch screen on the right side of the instrument, you must reposition the touch screen’s pivot arm assembly. See Repositioning the Touch Screen Pivot Arm Assembly on page 31 for more information.
To attach the touch screen when the NGC system is on a lab bench
1.Determine the side of the NGC instrument (right or left) on which to attach the touch screen.
2.Remove the side mount cover from the side of the instrument.
24 NGC Chromatography Systems and ChromLab Software
Attaching the Touch Screen
Note: The top section in this image is a third tier. (The NGC Discover system is a 3-tier system.) The touch screen is attached at the top of the second tier. If you have an NGC Quest or an NGC Scout system, you will not have a third tier. Locate the side mount cover at the top of the second tier.
3.Using a Phillips screwdriver, remove the screw that secures the cable trough cover to the back of the instrument.
Note: Store these components in a safe place for future use.
Installation Guide |
25 |
3Setting Up NGC Chromatography Systems
4.Using two ¼-20 x ¾" hex bolts and the hex key labeled pumphead provided in the NGC accessories kit, attach the touch screen mount to the side of the NGC instrument.
5.Slide the touch screen display onto the mounting post.
6.Insert a ¼-20 x ¾" hex bolt underneath the mount to secure the touch screen.
26 NGC Chromatography Systems and ChromLab Software
Attaching the Touch Screen
7.Locate the touch screen display cable in the accessories kit.
8.Firmly push the touch screen cable into the port underneath the touch screen and tighten the thumbscrews.
Tip: You can rotate the monitor if you need more room to insert the cable.
Installation Guide |
27 |
3Setting Up NGC Chromatography Systems
9.Route the display cable through the side and rear cable troughs on the instrument.
10.Insert the touch screen cable into the touch screen connector port on the back of the instrument and tighten the thumbscrews.
28 NGC Chromatography Systems and ChromLab Software
Loading...