Gel Doc XR |
ChemiDoc XRS |
170-8170 |
170-8070 |
170-8171 |
170-8071 |
Hardware Instruction Manual
MI170-8062BB Rev.G
Important
Please read these instructions before attempting to install or operate the Gel Doc XR or ChemiDoc XRS.
Note: This instrument is suitable for research use only.
It must be used, therefore, only by specialized personnel that know the health risks associated with UV radiation and with the reagents that are normally used with this instrument. Use of the acrylic screen doesn’t guarantee protection of the user from UV radiation. The use of protective eyeglasses or mask and gloves is strongly recommended.
Wichtig
Bitte lesen Sie die Anweisungen und Machen Sie sich mit der Bedienungsweise vertraut , bevor Sie den Hood benutzen.
Anmerkung: Dieses Geraet ist nur fuer Forschungszwecke geeignet.
Ausserdem ist die Benutzung nur fuer spezialisiertes Personal gedacht, das mit den Gesundheitsrisiken vertraut ist, die an die UV-Strahlung gebunden sind und den Reagentien, die normalerweise mit diesem Geraet benutzt werden.
Die Benutzung eines Plexiglasschutzschildes garantiert dem Benutzer keinen Schutz vor UVStrahlung. Die Benutzung von Schutzbrille oder Schutzmaske ist strengstens empfohlen.
Warranty
The Gel Doc XR, and Chemi XRS are warranted against defects in materials and workmanship for 1 year. If any defect occurs in the instrument during this warranty period, Bio-Rad Laboratories will repair or replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded:
•Defects caused by improper operation.
•Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized agent.
•Use of spare parts supplied by anyone other than Bio-Rad Laboratories.
•Damage caused by accident or misuse.
•Damage caused by disaster.
•Corrosion caused by improper solvents or samples.
Garantie
Die Garantie fuer den Hood betraegt 1 Jahr auf Herstellungsund Materialfehler.
Bei Auftreten von Fehlern waehrend der Garantiezeit repariert oder ersetzt Bio-Rad Laboratories die fehlerhaften Teile auf eigene Kosten. Die folgenden Schaeden sind in jedem Falle ausgeschlossen:
•Schaeden durch unsachgemaesse Bedienung bedingt.
•Instandsetzungen oder Veraenderungen durch nicht authorisiertes Personal von Bio-Rad Laboratories
•Benutzung von Ersatzteilen, die nicht von Bio-Rad Laboratories geliefert wurden.
•Schaeden durch Naturkatastrophen verursacht.
•Schaeden durch unsachgemaessen und fehlerhaften Gebrauch verursacht.
•Korrosionsschaeden durch ungeeignete Loesungen oder Proben.
Page 2 of 42
Regulatory Notice
IMPORTANT: The Gel Doc XR, and ChemiDoc XRS are designed and certified to meet ENC1010, the internationally accepted electrical safety standards and EMC regulations and TUV. Certified products are safe to use when operated in accordance with the instruction manual. This instrument should not be modified or altered in any way. Modification or alteration of this instrument will:
1.Void the manufacturer’s warranty.
2.Void the regulatory certifications.
3.Create a potential safety hazard.
NOTE: Bio-Rad Laboratories is not responsible for any injury or damage caused by use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent.
Richtlinien
WICHTIG: Dieses Geraet, von Bio-Rad Laboratories konstruiert, besitzt das Zertifikat ENC1010 anerkannt von dem internationalen elektrischen Sicherheitsstandard EMC und unterliegt dessen Richtlinien und TUV. Die Geraete sind sicher im Gebrauch, wenn die vorliegende Bedienungsanleitung beachtet wird. Dieses Geraet darf auf keinen Fall veraendert werden. Eventuelle Veraenderungen fuehren zu:
1.Ungueltigkeit der Herstellergarantie
2.Ungueltigkeit des Zertifikats
3.Eventuelles Sicherheitsrisiko
Bemerkung: Bio-Rad Laboratories haftet nicht fuer Verletzungen an Personen oder Sachschaeden, hervorgerufen durch eine nicht vorhergesehene Benutzung des Geraetes. Ebenso uebernimmt BioRad Laboratories keine Haftung fuer Veraenderungen, die von nicht authorisierten Personen durchgefuehrt wurden.
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General Precautions
•Please read the instruction manual carefully.
•The instrument must be used only for the intended purpose of gel documentation in research laboratories.
•The instrument must be connected to a power source line which MUST BE grounded and protected by a circuit breaker.
•Do not pour liquids directly on or inside the instrument.
•Switch off all the lights immediately after use.
•Clean the transilluminator platen after use.
Generelle Vorsichtsmassnahmen
•Bitte die Bedienungsanleitung aufmerksam lesen.
•Das Geraet darf nur fuer die vorgesehenen Applikationen eingesetzt werden, d.h. Geldokumentation in Forschungslaboratorien.
•Fuer die Stromversorgung des Hood muss eine geerdtete Steckdose benutzt werden.
•Keine Fluessigkeiten auf oder in das Geraet giessen.
•Die Lampe sofort nach dem Gebrauch ausschalten.
•Den UV-Filter des transilluminators nach Gebrauch reinigen.
Page 4 of 42
TABLE OF CONTENTS |
|
|
HARDWARE INSTRUCTION MANUAL .................................................................................................................. |
1 |
|
SECTION 1 INTRODUCTION..................................................................................................................................... |
7 |
|
1.1 |
COMPUTER REQUIREMENTS ...................................................................................................................................... |
7 |
1.2 |
PC ........................................................................................................................................................................... |
7 |
1.3 MACINTOSH .............................................................................................................................................................. |
7 |
|
SECTION 2 IMPORTANT SAFETY INFORMATION............................................................................................. |
8 |
|
2.1 |
IMPORTANT NOTICE .................................................................................................................................................. |
8 |
2.2 |
POWER SAFETY INFORMATION ................................................................................................................................... |
8 |
SECTION 3 PRODUCT DESCRIPTION .................................................................................................................... |
9 |
|
3.1 CCD CAMERA AND LENSES ....................................................................................................................................... |
9 |
|
3.2 |
DARKROOM ENCLOSURE, EMISSION FILTERS, AND ILLUMINATION SOURCES ............................................................... |
9 |
3.3 |
PCI INTERFACE CARDS .......................................................................................................................................... |
10 |
3.4 QUANTITY ONE SOFTWARE ................................................................................................................................... |
10 |
|
3.5 |
PRINTER (OPTIONAL) .............................................................................................................................................. |
10 |
3.6 |
PACKAGING ............................................................................................................................................................ |
10 |
SECTION 4 GETTING STARTED ............................................................................................................................ |
12 |
|
4.1 |
SELECTING A LOCATION .......................................................................................................................................... |
12 |
4.2 |
ASSEMBLING THE SYSTEM ........................................................................................................................................ |
12 |
4.2.1 Assembling the Universal Hood II ................................................................................................................. |
12 |
|
4.2.2 Assembling the CCD Camera ........................................................................................................................ |
13 |
|
4.2.3 Connecting the cabling harnesses.................................................................................................................. |
15 |
|
4.2.4 Installing the White Light Transilluminator (Optional)............................................................................... |
16 |
|
4.2.5 Installing the Optional 17 mm or 25 mm Wide-Angle Lenses (ChemiDoc XRS only):.............................. |
16 |
|
4.3 Description of System Function and System Initialization................................................................................ |
18 |
|
4.3.1 Control panel ................................................................................................................................................. |
18 |
|
4.3.2 System Initialization ....................................................................................................................................... |
21 |
|
4.3.3 Aligning the Camera assembly: ..................................................................................................................... |
24 |
|
SECTION 5 OPERATION OF THE UNIVERSAL HOOD II................................................................................. |
25 |
|
5.1 |
OPERATING THE UNIT .............................................................................................................................................. |
25 |
5.1.1 Switch on the Universal Hood II.................................................................................................................... |
25 |
|
5.1.2 Position your gel ............................................................................................................................................ |
25 |
|
5.1.3 Acquire an image ........................................................................................................................................... |
25 |
|
5.1.4 Acquiring an image with Flat Fielding:......................................................................................................... |
25 |
|
5.2 |
CUTTING GELS ........................................................................................................................................................ |
28 |
SECTION 6 TROUBLE SHOOTING......................................................................................................................... |
29 |
|
SECTION 7 ACCESSORIES AND REPLACEMENT PARTS............................................................................... |
30 |
|
Accessories and Replacement Parts........................................................................................................................ |
30 |
|
SECTION 8 MAINTENANCE AND PART REPLACEMENT............................................................................... |
32 |
|
8.1 |
EPI-ILLUMINATION LAMP REPLACEMENT .................................................................................................................. |
32 |
8.2 |
FUSE REPLACEMENT................................................................................................................................................ |
32 |
8.3 UV TRANSILLUMINATOR .......................................................................................................................................... |
33 |
|
8.3.1Lamp replacement........................................................................................................................................... |
33 |
|
8.3.2 Starter replacement (P/N 100-1370).............................................................................................................. |
34 |
|
APPENDIX A: GEL DOC XR SYSTEM CABLE CONFIGURATION.............................................................. |
35 |
|
APPENDIX B: CHEMIDOC XRS SYSTEM CABLE CONFIGURATION ....................................................... |
36 |
|
APPENDIX C: INSTALLING SOFTWARE AND DRIVERS ................................................................................ |
37 |
|
Gel Doc XR and ChemiDoc XRSPC: ............................................................................................................. |
37 |
|
Gel Doc XR and ChemiDoc XRSMac: ........................................................................................................... |
39 |
|
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ALSO READ THE RELEASE NOTES FILE FOR LAST MINUTE UPDATES FOR THE SOFTWARE. |
......40 |
APPENDIX D: PCI CARD INSTALLATION........................................................................................................... |
41 |
Gel Doc XR – PC:................................................................................................................................................ |
41 |
Gel Doc XR - Mac: .............................................................................................................................................. |
41 |
ChemiDoc XRSPC:............................................................................................................................................ |
41 |
Installing the PCI Digitizing Card .......................................................................................................................... |
41 |
APPENDIX E: TECHNICAL SPECIFICATIONS ................................................................................................... |
42 |
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Section 1 Introduction
The Bio-Rad Gel Doc XR, and the ChemiDoc XRS Gel Documentation systems are easy- to-use, high-performance systems. They use a CCD Camera to capture images in real time, which allows you to more accurately position and focus the image. While using Bio-Rad Quantity One software, acquired images can be optimized, annotated, analyzed, and printed.
The Bio-Rad Gel Doc XR, and the ChemiDoc XRS systems utilize an enclosure (Universal Hood II) that is light tight and contains UV illumination and white light illumination, Please note that both the Gel Doc XR and ChemiDoc XRS Cameras have external power supplies.
1.1 Computer Requirements
This software will run under Windows 2000 or XP, or on a Macintosh PowerPC.
The amount of computer memory required for using the program is mainly determined by the size of the images you will scan and analyze. Images scanned at high resolution can be quite large. For this reason, we recommend that you archive images on a network file server or removable storage media.
1.2 PC
The following is the recommended system configuration for installing and running on a PC:
Operating system: |
Windows 2000, Windows XP, |
Processor: |
Intel Pentium 400 MHz or better |
RAM: |
128 MB or more for Gel Doc XR, and ChemiDoc XRS systems. |
Hard disk space: |
3 GB or greater |
Monitor: |
17" monitor or better, 1024 x 768 resolution (absolutely required), True |
Printer: |
color. |
Optional. |
1.3 Macintosh
The following is the recommended system configuration for installing and running on a Macintosh:
Operating system: |
System Mac OS 10 |
Processor/Model: |
PowerPC G3 processor or better. |
RAM: |
256 MB or more for Gel Doc XR, and ChemiDoc XRS systems. |
Hard disk space: |
3 GB |
Monitor: |
17" monitor, 1024 x 768 resolution (absolutely required), Millions of |
Printer: |
colors. |
Optional. |
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Section 2 Important Safety Information
2.1 Important Notice
Use of the Gel Doc XR, and ChemiDoc XRS involves UV illumination. Proper precautions must be taken to avoid eye and skin exposure to the UV radiation. This instrument is meant for use only by specialized personnel that know the health risks associated with UV radiation and the reagents that are normally used with this instrument. The acrylic shield provides some UV protection. However, it does not guarantee complete protection, and it is designed to shield only the person working in front of the system.
WARNING: The operator should wear appropriate safety glasses or a protective mask and gloves in addition to using the UV Safety Shield provided with this instrument.
Anmerkung: Dieses Geraet ist nur fuer Forschungszwecke geeignet.
Ausserdem ist die Benutzung nur fuer spezialisiertes Personal gedacht, das mit den Gesundheitsrisiken vertraut ist, die an die UV-Strahlung gebunden sind und den Reagentien, die normalerweise mit diesem Geraet benutzt werden.
Die Benutzung eines Plexiglasschutzschildes garantiert dem Benutzer keinen Schutz vor UVStrahlung. Die Benutzung von Schutzbrille oder Schutzmaske ist strengstens empfohlen.
2.2Power Safety information
a)Voltage Setting Information: The Universal Hood II has a power supply that automatically chooses the correct voltage for your country or region.
b)Fusing: The Universal Hood II has two user serviceable fuses. These are located at the following location:
•F1 and F2 are located on the bottom rear panel and are a part of the power entry module, please see picture below for details:
Fuse F1 and F2 are located in this drawer. Use a screwdriver and pull the drawer out to see the fuses
Main power entry module
Page 8 of 42
Section 3 Product Description
3.1 CCD Camera and Lenses
A CCD Camera is placed on top of the Universal Hood II for capturing images. The Camera comes with a Motorized Zoom Lens (MZL) that allows a remote adjustment of the lens control functions viz. zoom, focus and iris.
For ChemiDoc XRS systems optional lenses with low F-number are available that offer extreme light collection efficiency for low light applications like chemiluminescence.
Lens f 0.95, 25 mm, Wide Angle |
1708072 |
Lens f 0.95, 17 mm, Wide Angle |
1708073 |
3.2 Darkroom Enclosure, Emission Filters, and Illumination Sources
The Universal Hood II is designed to capture fluorescence and chemiluminescence images without using a photographic darkroom. The enclosure has built-in white light epi-illumination and a UV transillumination. An optional White Light Transilluminator (170-7950) or a White Light Conversion Screen (170-8001) may also be used with the system.
For easy sample loading, the UV transilluminator is located in the drawer of the Universal Hood II and can accessed from the front of the enclosure. For your convenience, the lights in the darkroom enclosure turn off automatically after about 15 minutes. This time period can be extended indefinitely by pressing the HOLD button.
The Universal Hood II has a three position Filter Slider that offers the flexibility of using two different emission filters for fluorescence applications as well as the ability to image chemiluminescence samples (no filter).
Bio-Rad Laboratories offers a selection of filters and illumination sources.
Standard Filter: |
Comes with each system. |
|
548-630nm |
Amber Filter (Ethidium Bromide) |
170-8081 |
Optional Filters: |
Can be purchased for Optimal performance. |
|
520DF30 nm |
Optional Filter for SYBR Green/ SYBRGold/ GFP/ |
|
|
Fluorescein |
170-8074 |
560DF50 nm |
Optional Filter for CY3/Rhodamine/Sypro Orange |
170-8075 |
630BP30 nm |
Optional Filter for SYPRO Ruby/Texas Red |
170-8076 |
480BP70 nm |
Optional Filter for Hoechst/Coumarin |
170-8077 |
Optional Illumination Sources: |
|
|
White Light Transilluminator |
170-7950 |
|
White Light Conversion Screen |
170-8001 |
|
302 nm UV lamps 6 ea. (shipped standard with each system) |
170-8097 |
|
254 nm UV lamps 6 ea |
|
170-8098 |
365 nm UV lamps 6 ea |
|
170-6887 |
Page 9 of 42
3.3 PCI interface Cards
Gel Doc XR:
The Gel Doc XR system requires a Fire Wire port to communicate with the computer where the user interface software is installed. In case of computers that do not offer a Fire Wire (IEEE1394) interface, a PCI Fire Wire card (included with the system) needs to be installed in the computer to communicate with the Camera.
Note: Read and follow the instructions for installing the PCI card, drivers and Software in Appendix C and D.
ChemiDoc XRS:
The ChemiDoc XRS system requires a PCI Digitizing card to communicate with the Camera. This card needs to be installed in all PC and Mac systems with appropriate drivers (included in the software).
Note: Read and follow the instructions for installing the PCI card, drivers and Software in Appendix C and D.
3.4 Quantity One Software
Each system ships with 1 full version of Quantity One and unlimited copies of Quantity One Basic Mode. The software can be used to annotate and document images, analyze molecular weights, print, and perform a host of other applications. See the software manual for detailed instructions on how to install and operate the software.
Note: Read and follow the instructions in Appendix C and D prior to installing Quantity One software.
3.5 Printer (Optional)
For your convenience, Bio-Rad offers an optional USB printer for use with the Gel Doc XR and ChemiDoc XRS:
Sony UPD895 (recommended for both the systems) |
170-8066 |
3.6 Packaging
The Gel Doc XR, or ChemiDoc XRS systems consist of multiple components. Each of them may be in a separate box or in one box with a label describing the catalog numbers and its contents. Please make sure that all the system components are in your shipment. Please unpack each component carefully and verify that you have the correct one. Each system includes the following:
Page 10 of 42
Part # |
Descriptions |
170-8170/170-8171 |
Gel Doc XR System PC/Mac |
170-8062 |
Universal Hood II W/TLUM 100/240 Vac |
170-8172 |
Gel Doc XR Camera and Cable w/MZL |
170-8173 |
Gel Doc XR Fire Wire card (PC System only) |
170-8080 |
Kit Accessory, Universal Hood II |
170-7964/7963 |
Cable, Serial, MZL, PC/Mac |
170-9600 |
Quantity One Software PC/Mac |
170-8009 |
Adapter, USB to Serial (Mac System only) |
Part # |
Descriptions |
170-8070/170-8071 |
ChemiDoc XRS System PC/Mac |
170-8062 |
Universal Hood II W/TLUM 100/240 Vac |
170-8088 |
ChemiDoc XRS Camera W/MZL (including |
|
PCI card and Camera Controller Cable) |
170-8080 |
Kit Accessory, Universal Hood II |
170-8008 |
Fluorescent Reference Plate |
170-7964/7963 |
Cable, Serial, MZL, PC/Mac |
170-9600 |
Quantity One Software PC/Mac |
170-8009 |
Adapter, USB to Serial (Mac System only) |
Page 11 of 42
Section 4 Getting Started
4.1 Selecting a Location
The Gel Doc XR and ChemiDoc XRS systems can be placed on any bench top. The best position is near the computer where Quantity One will be operating. Remember to leave space to easily access the power switch on the Universal Hood II, which is located at the rear bottom left side of the enclosure
4.2Assembling the System
4.2.1Assembling the Universal Hood II
Important: It is recommended that the Universal Hood II be carried by at least two people holding the instrument from the bottom side. Be sure that the door and the drawer are completely closed.
Wichtig: Es wird empfohlen , dass mindestens zwei Personen den Hood transportieren und dabei das Geraet am Geraeteboden halten.
a)Open the box and review the unpacking instructions.
b)Carefully remove all of the components of your system.
c)Remove the top foam and the plastic wrapping from the Universal Hood II.
d)Carefully pull the enclosure out of the bottom foam and place it in a suitable location.
Note: Please do not connect the power cable to a power source until all connections are made. The power source has to be grounded and protected by a circuit breaker.
e)Locate the Amber Filter in the Accessory Kit and thread it onto one of the two 62 mm rings of the Filter Slider (inside of the hood). The Filter Slider facilitates the selection between two filters and an open position for chemiluminescence.
f)The three selectable positions are indicated by three symbols O (no filter), I (filter 1) or II (filter 2) on the right side of the Camera support. Note the position of the Amber Filter for future use. See pictures below.
Three positions sliding filter
Page 12 of 42
4.2.2 Assembling the CCD Camera
Gel Doc XR
a)Place the Camera on the bench so that the two tripod mount holes are facing you.
b)Using one of the two knurled screws and washers attach the Gel Doc bracket to the Camera as shown in the picture below. Use the tripod mounting hole that is closest to the top of the Camera. Do not tighten the screws yet.
c)The black adapter ring provided (3 millimeters thickness) has a soft and a hard side to it.
d)Position this adapter plate on top of the Universal Hood II such that the soft side of the ring is facing up and the ring is concentric with the round opening on the universal hood.
Gel Doc XR
Bracket
Gel Doc XR bracket, washers, screws and adapter |
Gel Doc XR Camera and bracket |
e)Place the Camera over the Universal Hood II by inserting the lens into the adapter ring.
f)Make sure that the front of the lens is in direct contact with the black adapter ring.
g)Verify that the lens is centered in the opening on top of the enclosure. To do this you may need to move the entire body of the Camera and the ring so that the lens is also in the center of the adapter ring.
WARNING: Failing to center the lens in the adapter ring and the hood can cause the focusing ring located on the front side of the lens to mechanically rub or bind.
h)Secure the Gel Doc XR bracket (attached to the Camera) to the bracket of the Universal Hood II using the screw and knurled knob (see pictures below)
i)Tighten the two screws of the Gel Doc XR Camera bracket. Check that the lens is well positioned on the black adapter ring for a complete light seal.
j)A completely assembled Camera will look like the picture shown below.
Screw, washer and knurled knob |
Gel Doc XR Camera assembled |
Page 13 of 42
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