Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 10 and 50 ml
Instruction Manual
Catalog #
732-5304, 732-5312, 732-5314
Table of Contents
Section 1 |
...Introduction ........................................ |
1 |
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Section 2 Connecting to Bio-Rad’s |
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Low-Pressure Chromatography |
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Instruments ...................................... |
24 |
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Section 3 |
Connecting to Other Liquid |
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Chromatography Systems .................. |
9 |
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3.1 |
Connecting to BioLogic |
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DuoFlow™ Systems ................. |
9 |
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3.2 |
HPLC Systems ...................... |
10 |
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3.3 |
FPLC Systems ....................... |
11 |
Section 4 |
Preparing a Cartridge for Use........... |
12 |
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4.1 |
Sample Preparation ............... |
13 |
Section 5... |
Desalting and Buffer Exchange......... |
14 |
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5.1With a Liquid Chromatography
System .................................. |
15 |
Section 6 Technical Assistance ........................ |
16 |
Section 7 |
Ordering Information |
.........................17 |
Section 8 |
References ....................................... |
19 |
Section 1
Introduction
Bio-Scale Mini cartridges have a patent-pending double-wall design that provides extra durability and allows easy, reliable runs with aqueous buffers commonly used for protein purification. The polypropylene luer fittings and internal sealing surfaces assure leak-free operation at pressures up to 45 psi. Bio-Scale Mini cartridges are convenient, disposable, and supplied ready for use. They are easy-to-use and prepacked for fast, reproducible chromatographic separations. Cartridges are available for a variety of chromatographic techniques, including desalting, ion exchange and affinity chromatography. The patent-pending design of Bio-Scale Mini cartridges offers:
•Supplied ready for use; simply equilibrate the cartridge in the buffer of choice
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•Luer fittings for convenient connection to any chromatography system
Bio-Scale Mini Bio-Gel P-6 cartridges are packed with Bio-Gel® P-6 gel. This gel consists of hydrophilic spherical polyacrylamide beads designed for the purification of proteins, nucleic acids, viruses, plasmids, and other macromolecules. The Bio-Gel P-6 beads are designed to provide high-efficiency desalting or buffer exchange at low backpressure. Detailed product information is given in Table 1. See Ordering Information for a listing of the complete Bio-Scale Mini cartridge product line.
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Table 1. Bio-Scale Mini Bio-Gel P-6 cartridge specifications.
Size |
10 and 50 ml bed volume |
Dimensions |
10 ml: 80 mm length x 12.6 mm inner |
|
diameter |
|
50 ml: 80 mm length x 28.2 mm inner |
|
diameter |
Maximum pressure tolerance |
10 ml: 45 psi |
|
50 ml: 40 psi |
Recommended flow rates: |
|
at ambient temperature |
|
(18–24°C) |
10 ml: 0.5–4.0 ml/min (24–192 cm/hr) |
|
50 ml: 2.5–20.0 ml/min (24–192 cm/hr) |
at cold room temperature |
|
(4–8°C) |
10 ml: 0.5–2.0 ml/min (24–96 cm/hr) |
|
50 ml: 2.5–10.0 ml/min (24–96 cm/hr) |
Fittings |
Female luer-lock fitting inlet and |
|
male luer fitting oulet |
Column material |
Polypropylene |
Frit material |
Polyethylene (HDPE) |
Shipping conditions |
20 mM Bis-Tris, pH 6.5 + 0.05% azide |
Storage recommendation |
20 mM Bis-Tris, pH 6.5 + 0.05% azide |
Autoclavability |
Not autoclavable |
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Section 2
Connecting to Bio-Rad’s Low-Pressure Chromatography Instruments
Bio-Scale Mini cartridges are ideal for use with Bio-Rad's BioLogic™ LP chromatography system, Econo™ gradient pump, the patented* Model EP-1 Econo™ pump, and all low-pressure chromatography instruments. Bio-Scale Mini cartridges can be conveniently connected directly to the system using the luer fittings on the cartridge.
1.Install 1.6 mm inner diameter (ID) tubing in the pumphead. Adjust platen pressure screw (on pumphead) – using a screwdriver or coin, turn the screw counterclockwise as far as it will go, then turn clockwise three full turns. Assemble with fittings and lock rings as shown in Figure 1.
*US Patent 5,135,658
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