Leica ST4040 User Manual

Leica ST4040

Linear Stainer

Instruction Manual

Leica ST4040 V1.4 - Rev A, English – 06/2009

Always keep this manual near the instrument.

Read carefully prior to operating the instrument.

IMPORTANT NOTE

The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.

For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual.

Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our prod-

ucts. These are determined only by the contract provisions agreed between ourselves and our customers.

Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.

This document is protected under copyright laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument.

© Leica Biosystems Nussloch GmbH

Issued by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch		Serial No.: .............. ......................................................
Germany
		Year of Manufacture: ...............................................
Phone:	[phone]
Fax:	[phone]	Country of Origin: Federal Republic of Germany
Internet:	http://www.microsystems.com

Leica ST4040 – Linear Stainer

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Table of Contents

IMPORTANT NOTE...........................................................................................................................................................................................			3
1.		Important Information ..........................................................................................................................................................................	6
1.1	Symbols used in this manual and .......................................................................................................................................................		6
1.2		Designated use ......................................................................................................................................................................................	7
2.		Safety.......................................................................................................................................................................................................	8
2.1		Safety instructions ................................................................................................................................................................................	8
2.1.1		Transport and installation ...................................................................................................................................................................	8
2.1.2		Operating the instrument .....................................................................................................................................................................	9
2.1.3		Cleaning and maintenance ...............................................................................................................................................................	10
3.		Installation ...........................................................................................................................................................................................	11
3.1		Site requirements ................................................................................................................................................................................	11
3.2		Unpacking the instrument ..................................................................................................................................................................	12
3.2.1		Repacking the instrument .................................................................................................................................................................	12
3.3		Installing the instrument .....................................................................................................................................................................	13
3.4	Standard delivery ‘basic instrument’ and general accessories .................................................................................................		14
3.4.1		Standard delivery ................................................................................................................................................................................	14
		Basic instrument ST4040 - single load model ................................................................................................................................	14
		Basic instrument ST4040 - double load model (for double row staining) .................................................................................	14
3.4.2		General accessories ..........................................................................................................................................................................	15
3.5		Electrical connections ........................................................................................................................................................................	16
3.5.1 Adjusting the voltage selector..........................................................................................................................................................			16
3.5.2 Connecting the power chord to the .................................................................................................................................................			17
3.6		Installing the accessories ..................................................................................................................................................................	17
3.6.1 Installing the water inlet hose ..........................................................................................................................................................			17
3.6.2 Installing the drain hose ....................................................................................................................................................................			17
3.6.3 Installing the exhaust air hose .........................................................................................................................................................			17
3.6.4		.....................................................................................................................................................................	Placing the instrument in its
		........................................................................................................................................................................................................................
		.....................................................................................................................................................................................	permanent position
	18
3.6.5 Connecting the inlet hose to the tap................................................................................................................................................			18
3.6.6		Leveling the instrument .....................................................................................................................................................................	18
3.6.7 Inserting the activated carbon filter ................................................................................................................................................			19

3.7Plugging the power chord into

......................................................................................................................................................................................................				the wall outlet
19
4.	Installation of the Optional Load/Unload Station .........................................................................................................................			20
4.1	Site requirements ................................................................................................................................................................................			20
4.2	Unpacking the optional load /	unload station .......................................................................................................................		20
4.2.1 Repacking the optional load / unload			station ......................................................................................................................	20
4.3	Standard delivery and accessories - optional load / unload station ..........................................................................................			21
4.3.1 Standard delivery - optional load station .........................................................................................................................................				21
	Accessories - optional load station	.................................................................................................................................................		21
4.3.2 Standard delivery - optional unload station			...................................................................................................................................	21

4	Instruction Manual V1.4 – 06/2009

			Table of Contents
		Accessories - optional unload station ............................................................................................................................................	21
4.4		Installation ............................................................................................................................................................................................	22
4.4.1 Installing the optional load station ...................................................................................................................................................			22
4.4.2 Installing the optional unload station ..................................................................................................................................................
	on the right of the basic instrument .....................................................................................................................................................		25
4.5		Changing the guide ramps for slide rack carriers .........................................................................................................................	28
5.		Instrument Properties ........................................................................................................................................................................	29
5.1		Technical Data .....................................................................................................................................................................................	29
6.		Operation ..............................................................................................................................................................................................	30
6.1		Setting up the instrument ...................................................................................................................................................................	30
6.2		Preparing the instrument for staining: inserting and filling the stations ....................................................................................	30
6.3		Staining in two rows ...........................................................................................................................................................................	31
6.3.1 Doubling the specimen throughput .................................................................................................................................................			31
6.3.2 Carrying out two different, but matching, staining protocols simultaneously .........................................................................			31
6.3.3 Carrying out two different staining protocols (two subsequent single loads) .........................................................................			31
6.4		Control panel functions ......................................................................................................................................................................	32
6.5		Switching on mains and selecting direction of travel and alarm volume..................................................................................	33
...........................................................................................................................................................................................................................			33
6.5.1 Selecting direction of travel ..............................................................................................................................................................			33
...........................................................................................................................................................................................................................			33
6.5.2		Selecting alarm volume .....................................................................................................................................................................	34
...........................................................................................................................................................................................................................			34
6.6		Switching on mains without selecting direction of travel/alarm volume ..................................................................................	34
...........................................................................................................................................................................................................................			34
6.6.1		Stand-by mode ....................................................................................................................................................................................	34
...........................................................................................................................................................................................................................			34
6.7		Programming ........................................................................................................................................................................................	35
6.8		Staining .................................................................................................................................................................................................	36
6.8.1		Selecting a program ...........................................................................................................................................................................	36
...........................................................................................................................................................................................................................			36
6.8.2 Preparing and inserting the slide racks ..........................................................................................................................................			37
6.8.3 Starting the selected program..........................................................................................................................................................			38
...........................................................................................................................................................................................................................			38
6.9		Removing the slide racks ...................................................................................................................................................................	39
6.8.4		Interrupting a program .......................................................................................................................................................................	39
...........................................................................................................................................................................................................................			39
6.9.1 Removing the last slide rack .............................................................................................................................................................			40
...........................................................................................................................................................................................................................			40
6.10 Removing the slide racks in instruments equipped with optional unload station ...................................................................			40
...........................................................................................................................................................................................................................			40
6.11		Finishing work .....................................................................................................................................................................................	41
...........................................................................................................................................................................................................................			41
7.		Trouble Shooting.................................................................................................................................................................................	42
8.		Cleaning and Maintenance ..............................................................................................................................................................	44
8.1		Cleaning ................................................................................................................................................................................................	44
8.2		Maintenance ........................................................................................................................................................................................	46
Leica ST4040 – Linear Stainer			5
9.		Warranty and Service ........................................................................................................................................................................	47

1.Important Information

The instruction manual for the Leica ST4040 includes chapters on the following subjects:

Chapter 1		Structure of this manual
		•	Table of contents
		• Important information on this
			manual
Chapter 2		Safety
		• Read this chapter before
		operating the instrument
Chapter 3		Installation
		•	Unpacking and installation
		•	Standard delivery, accessory
			range
Chapter 4		Installation
		Optional load / unload station
		•	Unpacking and installation
		•	Standard delivery, accessory
			range
Chapter	5	Instrument features
		•	Technical data
Chapter	6	Operation

•Controls

•Setup and daily use

Chapter 7 Trouble Shooting

•Operating errors

•Trouble shooting

Chapter	8	Cleaning and Maintenance
Chapter	9	Warranty and Service
Chapter 10		EC Declaration of Conformity
Appendix 1		Staining protocols Leica ST4040

1.1Symbols used in this manual and their meaning

Warnings appear in a grey box and are marked by a warning triangle.

Notes

i.e. important user information appear in a grey box and are marked by an information symbol.

(5)Figures in brackets refer to item numbers in drawings or to the drawing it-

(Fig. 5) self.

Instrument type:

All information given in this instruction manual applies only to the instrument type indicated on the title page.

A name plate indicating the instrument serial number is attached to the back of the instrument.

Required information for all enquiries:

For any enquiries please specify:

•Instrument type

•Serial number

6	Instruction Manual V1.4 – 06/2009

1.Important Information

General

This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument.

The instruction manual is an important part of the product. It must be read carefully before first using the instrument and must always be kept with the instrument.

It will be necessary to add appropriate instructions to this instruction manual, if imposed by existing national regulations or by laws on accident prevention and environmental protection in the country of the operating authority.

Read this instruction manual carefully before attempting to use or operate the instrument.

Please pay particular attention to the safety instructions in chapter 2. Please do read this information, even if you are familiar with the operation and use of Leica products.

1.2Designated use

The Leica ST4040 is an automated linear stainer for the preparation of histological and cytological routine stainings.

It is designed for use in pathology laboratories, and only for performing the following tasks:

•Staining of thin sections of tissue specimens or of cytological samples, attached to microscope slides

Any use of the instrument other than its designated use is considered improper.

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2.Safety

2.1Safety instructions

This instrument has been built and tested in accordance with the following safety regulations on electrical measuring, control, regulating and laboratory devices.

In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this instruction manual.

For current information about applicable standards, please refer to the CE declaration of conformity on our Internet site:

www.histo-solutions.com

2.1.1 Transport and installation

•Do not operate the instrument in rooms where risk of explosion exists!

•Do not expose the instrument to direct sunlight (windows)!

•Do not install the instrument above a heater!

•Install the instrument on an even laboratory bench which must be absolutely level!

•Two people are needed to lift / carry the instrument!

•Before connecting the instrument to mains, make sure the correct voltage setting, matching the nominal voltage at the site of installation, has been selected!

•Upon installing the drain hose make sure there is a gradient from the drain outlet to the waste pipe.

•To protect the user from hazardous solvent fumes, make sure to operate the instrument either with the activated carbon filter or the exhaust air hose!

8	Instruction Manual V1.4 – 06/2009

2.Safety

2.1.2 Operating the instrument

•The instrument may only be operated by skilled personnel. It may only be operated according to its designated use and in accordance with the instructions given in this manual!

•While working with reagents (filling / emp-ty- ing the reagent stations, working on the instrument while the lid/s is/are open) appropriate protective gear (lab coat, gloves, safety goggles) must be worn!

•Make sure to operate the instrument ei-ther with the activated carbon filter or with the exhaust air hose (--> Chapter 3.6, ‘Installing the accessories’). Even when the instrument is operated according to its designated use, hazardous solvent fumes develop, which are damaging to the operator’s health and do also pose a risk of fire!

•Risk of fire, when working with an open flame (Bunsen burner) immediately next to the instrument (solvent fumes)! - Therefore, keep a safety distance of 1 meter!

•If ‘Alarm off’ is selected (not recommended!), the instrument must be observed constantly, to make sure all slide racks are removed immediately when reaching the last station!

•If not only the water tap but also the ball valve has been closed (--> e.g. when setting up the instrument), the tap water flow rate has to be adjusted when starting a program (see also chapters 3.6 and 6.8.3).

•The flow rate must not be too fast, to en-sure that the specimens remain firmly at-tached to the slide surface!

•If a staining program is to be interrupted for an extended period of time, do not leave any slide racks in the tap water sta-tions, in order to prevent them from drying out!

•As soon as the alarm is triggered, immediately remove the completed slide rack from the last station or from the unload container of the optional unload station! Otherwise, immersion times for the remaining slide racks will be prolonged!

•In case of emergency switch off mains and unplug the power chord!

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2.Safety

2.1.3 Cleaning and maintenance

•Only technical service engineers authorized by Leica may access the internal components of the instrument for service and repair.

Exception: Changing the activated carbon filter is the only maintenance task to be carried out by the user.

•Prior to cleaning the instrument, always switch off mains and unplug the power chord!

•Dispose of used reagents according to the laboratory regulations in force in your country!

•Spilled solvents (reagents) have to be wiped away immediately! - In case of long-term exposure, the lid surfaces are only conditionally resistant to solvents!

•The painted surfaces and the control panel are not resistant to xylene or acetone!

•For cleaning the instrument, do not use any one of the following: alcohol, detergents containing alcohol (window cleaner!), abrasive cleaning powders, solvents containing xylene or acetone!

•To clean the lids, control panel and housing, use mild household detergents; - see safety instruction above for non-appropriate ingredients!

•When handling cleaning detergents, follow the instructions of the manufacturer and make sure all applicable laboratory regulations are complied with!

•When cleaning the instrument, no liquid may enter in contact with any of the electrical connections or get into the interior of the instrument!

•Wash the tap water and reagent stations in the dishwasher at a temperature of max. +65 °C. - Use a standard detergent for laboratory dishwashers.

At any rate avoid washing the stations at higher temperatures (e. g. in industrial diswashers which run at a temperature of

+85 °C), as the stations may become deformed!

10	Instruction Manual V1.4 – 06/2009

3.Installation

3.1Site requirements

The installation site has to meet the following requirements:

•Stable laboratory bench, exactly level, at least 1.60 m wide and 60 cm deep.

•Tap water supply at a distance of max. 2 m and drain at a distance of max. 1.50 m from the corresponding inlet and outlet at the back of the instrument.

- Please bear in mind: The connections are located at the extreme left on the back of the instrument.

•Fume hood at a distance of max. 3.50 m from the instrument, if the instrument is to be operated with the exhaust air hose (otherwise the instrument must be operated with the activated carbon filter)!

•Vibration-free floor.

•Sufficient open space (70 cm) above the laboratory bench, to ensure trouble-free opening of the lids.

•Stable ambient temperature of +10 °C to +35 °C.

•Relative air humidity of maximum 80%, noncondensing.

•No other instruments which cause vibrations installed nearby.

Do not operate the instrument in rooms where risk of explosion exists!

Do not expose the instrument to direct sunlight (windows)!

Do not install the instrument above a heater!

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3.Installation

3.2Unpacking the instrument

The unpacking instructions for all Leica instruments are located in a transparent protective envelope on the outside of the instrument transportation crates.

3.2.1 Repacking the instrument

We recommend keeping the original crate and packing material in store, in case the instrument needs to be repacked in the future.

The drawing below shows the design of the original crate and packing material. The numbers indicate the sequence of disassembling and reassembling the crate.

9

8

7

6

5

4

3

2

1

12	Instruction Manual V1.4 – 06/2009

3. Installation

3.3Installing the instrument

•To lift, grip the instrument by the transport handles.

Two people are needed to lift / carry the instrument, since it weighs 73 kg (see ‘Technical data’, chapter 5.1)!

•Install the instrument on the designated bench.

•Unscrew the transport handles.

•Remove the plastic protective cover from the instrument.

•Check all delivered parts against the packing list to verify whether the delivery is complete - see chapter 3.4 ‘Standard delivery ‘basic instrument’ and general accessories’).

•For all further installation steps, see chapters 3.5 ‘Electrical connection’ and 3.6 ‘Installing the accessories’.

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3.Installation

3.4Standard delivery ‘basic instrument’ and general accessories

3.4.1Standard delivery

Basic instrument ST4040 - single load model

•Basic instrument with three-piece lid

•27 Reagent stations, plastic

•4 tap water stations, complete assy. with inlet valve

•1 Drain hose for waste water, 2 m long

•1 Tap water inlet hose, 2.50 m long,

complete with 3/4“ connection for water tap and replacement gasket

•2 Lids for reagent stations

•1 Cover for second row

•1 Instruction manual ................................................................ 14 0474 80001

•1 Tool set, including:

•1 Single-ended open-jaw wrench, size 27

•1 Single-ended open-jaw wrench, size 13

•1 Screw driver, 5.5 x 200 mm

•1 Screw driver, 3 x 50 mm

•1 Water tap adapter for 1/2“ water taps

•3 Disposable plastic films, solvent-resistant, for control panel

•1 Set of power chords:

•Euro

•UK

•USA

Basic instrument ST4040 - double load model (for double row staining)

To use a single load model for double row staining, order the appropriate number (may vary - depends on individual staining applications) of the accessories listed below (see chapter 3.4.2 - ‘General accessories’ for details):

•Reagent stations

•Tap water stations

•Lids for reagent stations

•Slide racks

•Slide rack carriers

14	Instruction Manual V1.4 – 06/2009

3. Installation

3.4.2General accessories

•Reagent stations, plastic

•Tap water stations, complete assy. with inlet valve

•Leica slide racks, metal

•Sakura slide racks, plastic

•Adapter for large slides

•Drain hose, 4 m long

•Tap water inlet hose, 2.50 m long,

complete with 3/4“ connection for water tap

•Lids for reagent stations

•Slide rack carriers for Leica slide racks

•Slide rack carriers for Medite/Hacker slide racks

•Slide rack carriers for Sakura slide racks

•Storage container for slide rack carriers, fits on instrument housing

•Activated carbon filter

•Exhaust air hose, 2 m long

•Exhaust air hose, 4 m long

•Disposable plastic films, solvent-resistant, for control panel (set of 10)

•Optional load station, left*

•Optional load station, right*

•Optional unload station, left*

•Optional unload station, right*

•Leica CV5000 adapter for slide racks:

•CV5000 slide rack adapter, removable:

-slides onto Leica slide racks for coverslipping in Leica CV5000.

-up to Leica CV5000 s/nos. ≤ CV026096

•CV5000 stationary slide rack adapter:

-for permanent installation in Leica CV5000 for using Leica ST4040 slide racks in CV5000.

-starting from Leica CV5000 s/nos. > CV026096)

To order accessories, please contact your Leica local sales organization for the latest literature / current order numbers on the full range of Leica ST4040 accessories.

*) For accessories for optional load / unload station, please refer to chapters 4.3.1 and/or 4.3.2 respectively!

Leica ST4040 – Linear Stainer

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