Leica MZ16 F
User manual
Dear User,
We thank you for your faith in our product, and hope it brings you both enjoyment and success.
With the new fully apochromatic Leica MZ16 F from Leica Microsystems, you have purchased the best-performing, manual fluorescence stereomicroscope on the market. The Leica MZ16 F and its motorized counterpart, the Leica MZ16 FA, provide the greatest zoom (16:1), the highest resolution (up to 840 Lp/mm), the highest magnification (115× with standard optics), the largest numerical aperture (0.14), a patented illumination and filter system for the most intense fluorescence, and the innovative HL RCTM high-performance transmitted-light base for excellent contrast.
The entire imaging system, including the 16:1 zoom, objective and ErgoTube® is apochromatically corrected using with much technological effort. Contrast, sharpness, richness in detail, resolution, image and color fidelity are optimum. In addition, the patented illumination beam path guarantees at every zoom level that light utilization is at a maximum and that fluorescence images are intensely luminous on a jet black background.
In developing our instruments, we place great value on simple, self-explanatory operation. However, please take the time to read the user manual and the operating safety information so that you can become familiar with the advantages and features of your Leica MZ16 F high-performance stereomicroscope, so that you can use it optimally and safely.
Should you ever have any questions, please consult your local Leica representative. You will find the address of your nearest local representative, as well as valuable information about Leica Microsystems products and services, from Leica Microsystems on our web site at www.leica-microsystems.com. We are gladly at your service. Customer service is a big thing with us. Not only before the sale, but afterwards as well.
Leica Microsystems (Switzerland) Ltd. Stereo & Macroscope Systems www.stereomicroscopy.com
Leica2 |
MZ16 F – User manual |
Leica MZ16 F – User manual2 |
Operating controls
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Fig. 1 Leica MZ16 F with trinocular video/phototube and Leica digital camera
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Zoom magnification changer |
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Trinocular video/phototube |
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Coarse/fine focusing drive |
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Adjustable tubes for adjusting the |
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Double iris diaphragm |
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interpupillary distance from 52mm–76mm |
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Rapid filter changer for four selectable filter sets |
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Knurled rings for adjusting the diopter settings |
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Filter holder for an individually selectable filter |
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from +5 to –5 |
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Switch for activating/deactivating the |
10 Adjustable and attachable eyecups |
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UV shutter |
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Leica MZ16 F – User manual |
Contents
Page
1. Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . .5
1.1 The user manual . . . . . . . . . . . . . . . . . . . . . . . . .5 1.1.1 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . .5 1.1.2 Symbols used . . . . . . . . . . . . . . . . . . . . . .6 1.2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . .6
1.3 Light source . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
2. Overview of the instrument . . . . . . . . . . . . . .10
3. Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
3.1 Assembly sequence . . . . . . . . . . . . . . . . . . . .12 3.1.1 Expansion diagram . . . . . . . . . . . . . . . . .13
3.2 Assembly of stereomicroscope with stand . .14 3.3 Additional components . . . . . . . . . . . . . . . . . .15 3.3.1 Video/phototube . . . . . . . . . . . . . . . . . . .15
3.4 Light source . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 3.4.1 Lamp housing 106 z . . . . . . . . . . . . . . . . .15 3.4.2 Supply units . . . . . . . . . . . . . . . . . . . . . . .15
3.5 UV protection screen . . . . . . . . . . . . . . . . . . . .16
3.6 Equipping the filter changer . . . . . . . . . . . . . .16 3.6.1 Filter sets for fluorescence . . . . . . . . . .16 3.6.2 Simple filter holders . . . . . . . . . . . . . . . .17 3.6.3 Inserting the filter . . . . . . . . . . . . . . . . . .17 3.6.4 Slot for filter slide . . . . . . . . . . . . . . . . . .17
Page
4. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
4.1 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.1.1 Fluorescence technology . . . . . . . . . . .18
4.1.2 Functional principle . . . . . . . . . . . . . . . .18
5. Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
5.1 Stereomicroscope settings
and adjustments . . . . . . . . . . . . . . . . . . . . . . . .18 5.1.1 Observation without fluorescence . . . .19 5.1.2 Double iris diaphragm . . . . . . . . . . . . . .19 5.2 Commissioning the fluorescence system . .20
5.3 Rapid filter changer . . . . . . . . . . . . . . . . . . . . .20
6. Work flow . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
7. Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
7.1 Filter sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22 7.2 Technical data . . . . . . . . . . . . . . . . . . . . . . . . .23 7.3 Optical data . . . . . . . . . . . . . . . . . . . . . . . . . . .27 7.4 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
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Leica MZ16 F – User manual |
1. Safety concept
1.1 The user manual
Included with your Leica MZ16 F fluorescence stereomicroscope is an interactive CD-ROM with all pertinent user instructions in German, English, French, Spanish, Italian, Portuguese, Dutch, Danish, Swedish, Finnish and Greek. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.stereomicroscopy.com.
The Leica MZ16 F is the manual fluorescence stereomicroscope of the Leica M stereomicroscope series. This operating manual describes the special functions of the Leica MZ16 F and important instructions for its operational safety, maintenance, and accessories. Like all instruments of the Leica M stereomicroscope series, the Leica MZ16 F consists of modules, which allow an almost unlimited variety of custom equipment configurations. The identical modules, such as stands, binocular tubes, accessories, etc. are described in User Manual M2-105-0 for
Leica M stereomicroscopes, which also contains additional safety instructions pertaining to the stereomicroscope, accessories and electrical accessories, as well as care instructions.
Special manuals are provided for a number of accessories:
–User Manual M2-216-1 for lamp housing with high-pressure mercury burner.
–User Manual for ebq 100 isolated supply unit for 100W high-pressure mercury burners.
–User Manual M2-267-1
for the motorized focus system.
–User Manual M2-166-2 for FluoCombi IIITM
Before installing, operating or using the instruments, read the user manuals listed above. In particular, please follow all safety instructions.
To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.
1.1.1 Diagrams
(1.2) Numbers in parentheses within the descriptions refer to diagrams and the items in these diagrams. Example (1.2):
Figure 1 is located on page 3, and item 2 is the focusing drive.
Leica MZ16 F – UserSafetymanualconcept |
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1.1.2 Symbols used
Warning of a danger
This symbol indicates especially important information that, if not observed,
–can cause hazards to personnel,
–can lead to functional disturbances and damaged instruments.
Warning of hazardous electrical voltage
This symbol indicates especially important information that, if not observed,
–can cause hazards to personnel,
–can lead to functional disturbances and damaged instruments.
Danger due to hot surface
This symbol warns against touching hot surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional information or explanations that intend to provide clarity.
Action
This symbol refers to actions described in the text that are to be carried out.
Additional notes
•This symbol indicates additional notes and explanations provided in the text.
1.2 Safety instructions
Description
The Leica MZ16 F is a manual fluorescence stereomicroscope with 16:1 zoom for fluorescence applications. The optics system consists of
two observation beam paths, a patented TripleBeam® third beam path specifically for fluorescence illumination and the patented FLUOIII® filter system. The entire imaging system, including the 16:1 zoom, objective and ErgoTube® is apochromatically corrected.
The Leica MZ16 F consists of these modules:
–Stereomicroscope
–Stand, binocular tube, objective, eyepieces and (if applicable) accessories for photography, etc.
–Power supply
–Filter sets with barrier and excitation filters on one filter holder
–Three simple filter holders for transmitted light observation and protection from UV light
–Filter holder for individual filters
–UV protection screen
–106 z lamp housing with 50W or 100W highpressure mercury burner with stray-light protection
–Supply unit
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Leica MZ16 F – Safety concept |
Permitted uses
With the Leica MZ16 F fluorescence stereomicroscope, entire fluorescent specimens can be examined three-dimensionally and unprepared, manipulated, sorted and documented. The separate beam path for the fluorescence illumination in the Leica MZ16 F as well as the very bright high-pressure mercury burner and specially selected filter sets enable you to distinguish the finest structures and enhance the quantity of information for incident light fluorescence. Special filter holders also enable observation without fluorescence in transmitted light or incident light.
Non-intended use
If the Leica MZ16 F, its components and accessories are used other than as described in the user manual, injury or property damage may occur.
Never:
–install other plugs or cables.
–change, rebuild or take apart parts, if not specifically instructed to in this manual.
–allow unauthorized persons to open parts.
–use the MZ16 F for examining, or operating on the human eye.
The devices and accessories described in this User manual have been tested for safety and potential hazards. The responsible Leica affiliate or the main plant in Wetzlar must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual. Unauthorized alterations to the device or noncompliant use shall void all rights to any warranty claims!
Place of use
–The Leica MZ16 F may only be operated in a closed, dust-free room, which is free of oil vapors and other chemical vapors, as well as extreme humidity, and within a temperature range of +10 to +40.
–Electrical components must be assembled at least 10 cm away from the wall and from flammable substances.
–Large temperature fluctuations, direct sunlight and vibrations should be avoided. These conditions can distort measurements and micrographic images.
–Stereomicroscopes in warm and warm-damp climatic zones require special care in order to prevent the build up of fungus.
Responsibilities of the person(s) in charge of instrument
Ensure that
–the Leica MZ16 F and accessories are operated, maintained and repaired only by authorized and trained personnel.
–the operating personnel has read, understands, and is observing these instructions as well as those listed on page 5, and, in particular, the safety instructions.
Transport
–Use the original packaging for shipping or transporting the stereomicroscope and its accessory components.
–In order to prevent damage from vibrations, disassemble all moving parts that (according to the user manual) can be assembled by the customer and pack them separately. These include the objective, binocular tube, eyepieces, lamp housing, burner in the 106 z lamp housing, etc.
Leica MZ16 F – UserSafetymanualconcept |
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Repairs and servicing
–Only Leica Microsystems-trained service technicians, or technical specialists assigned by the person in charge of the instrument, are permitted to carry out repairs.
–Only original Leica Microsystems spare parts may be used.
–Disconnect the power cable before opening voltage-carrying parts such as the power supply or the supply unit.
Touching the live circuit can cause injury.
Installation in third-party products
When installing Leica products into third-party products, note the following:
The manufacturer of the complete system or its dealer is responsible for following all applicable safety instructions, laws and guidelines.
Disposal
–The product must be disposed of in accordance with locally applicable laws and regulations.
–Dispose of used high-pressure mercury burners as hazardous waste.
Legal requirements
Adhere to general and local regulations relating to accident prevention and environmental protection.
Conformity with European Community directive
The Leica MZ16 F and its accessories are constructed in accordance with the latest technologies and are provided with a statement of conformity with EC requirements.
Health risks
Workplaces with stereomicroscopes both improve and make visual tasks easier, but they also place high demands on the human visual apparatus and the muscular "holding apparatus" of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal problems may occur. For this reason, appropriate measures for reduction of the workload must be taken:
–optimal arrangement of workplace, work assignments and work flow (changing tasks frequently).
–thorough training of the personnel, giving consideration to ergonomic and organization aspects.
The ergonomic design and construction of Leica M series stereomicroscopes are intended to reduce the exertion of the user to a minimum.
Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye.
The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups.
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Leica MZ16 F – Safety concept |
1.3 Light source: safety instructions
Precautions by the manufacturer
–UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays.
–Simple filter holders (that have only two openings for the observation beam paths) in the available positions of the rapid filter changer prevent direct UV radiation from reaching the eyes.
–UV shutter (which can be activated/deactivated) prevents direct UV radiation from reaching the eyes.
–UV filters in the observation beam path protect the eyes.
–The stray-light protection on the lamp housing prevents irradiation of the hands.
Warning
UV radiation can harm the eyes.
Therefore:
–Never look into the light spot on the specimen plane without a UV protection screen.
–Always fill all of the filter positions with filter holders.
–Fill empty filter positions with the simple filter holders (which have only two openings for the observation beam paths).
–Do not select a white, strongly reflective background for the stage.
–Never open the lamp housing while the lamp is switched on.
Risk of explosion, UV exposure, blinding!
Supply unit
Always unplug the supply unit from the power supply:
–when installing and disassembling the lamp housing
–before opening the lamp housing
–when replacing the high-pressure mercury burner and other parts, such as the heatabsorbing filter or the collector
–during maintenance work on the supply unit.
Lamp housing
The Leica MZ16 F may be used only together with the Leica 106 z lamp housing for the 100W or 50W mercury burner, supply unit and stray-light protection.
Leica Microsystems accepts no liability for damages resulting from the use of lamp housings from other manufacturers.
–Never open the lamp housing while the lamp is switched on. Unplug the supply unit from the power supply:
Risk of explosion, UV exposure, blinding!
–Before opening the lamp housing, allow it to cool off for at least 15 minutes.
Danger of explosion, danger of burns.
–Never cover the air duct on the lamp housing.
Danger of fire!
–Use the stray-light protection.
Danger to eyes from UV radiation.
Leica MZ16 F – Safety concept |
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