Leica AutoStainer XL Instruction manual

Leica

AutoStainer XL

Automated slide stainer

Instruction Manual

Leica AutoStainerXL, V2.1 English – 10/2002

Always keep this manual near the instrument!

Read carefully prior to operating the instrument!

NOTE

The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state- of-the-art technology as we understand it following thorough investigation in this field.

We are under no obligation to update the present manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.

For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual.

Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products.

Published by:

Leica Microsystems Nussloch GmbH Heidelberger Str. 17 - 19

D-69226 Nussloch Germany

Phone: [phone] Fax: [phone]

eMail: [e-mail] Internet: http://www.histo-solutions.com

These are determined only by the contract provisions agreed between ourselves and our customers.

Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.

This document is protected under copyright laws. Any copyrights of this document are retained by Leica Microsystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Microsystems Nussloch GmbH.

For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument.

© Leica Microsystems Nussloch GmbH

Leica AutoStainer XL – Automated slide stainer

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Table of contents

1.	Important information ....................................................................................................		6
	1.1	Symbols occurring in the text and ............................................................................................	6
	1.2	Designated use / improper use of the instrument ..................................................................	7
	1.3	Qualification of personnel ..........................................................................................................	7

2.	Safety.................................................................................................................................		8
	2.1	Safety regulations .......................................................................................................................	8
3.	Instrument features ........................................................................................................		9
	3.1	Overview – instrument ..............................................................................................................	10
	3.2	Standard delivery - packing list ..............................................................................................	12
	3.3	Technical Data ...........................................................................................................................	13
4.	Installation .....................................................................................................................		14
	4.1	Site requirements ......................................................................................................................	14
	4.2	Connection..................................................................................................................................	14
	4.2.1	Power ..........................................................................................................................................	14
	4.2.2	Water supply ..............................................................................................................................	15
	4.3	Battery backup - UPS (optional)..............................................................................................	16
	4.4	Remote alarm (optional) ...........................................................................................................	17
	4.5	Fume control system .................................................................................................................	17
	4.6	Oven .............................................................................................................................................	17
5.	Operation ........................................................................................................................		18
	5.1	The control panel .......................................................................................................................	19
	5.2	The main menu ...........................................................................................................................	20
	5.3	Menu map ...................................................................................................................................	21
	5.4	Edit a program ............................................................................................................................	22
	5.4.1	Entering steps ............................................................................................................................	23
	5.4.2	Erasing steps ..............................................................................................................................	24
	5.4.3 Inserting a blank step into a program ....................................................................................		24
	5.4.4 Removing blank steps in a program .......................................................................................		25
	5.4.5	Saving a program ......................................................................................................................	25
	5.4.6	Deleting a program ....................................................................................................................	25
	5.4.7	Copying a program ....................................................................................................................	26
	5.4.8	Viewing a program ....................................................................................................................	27
	5.4.9	Checking program compatibility .............................................................................................	27
	5.5	User-adjustable parameters ....................................................................................................	29

4	Instruction manual V 2.1 – 10/2002

			Table of contents
	5.6	Oven .............................................................................................................................................	29
	5.7	Agitation (Dips) ..........................................................................................................................	30
	5.8	Rack movement times ...............................................................................................................	31
	5.9	Staining .......................................................................................................................................	31
	5.9.1	Reagent containers ...................................................................................................................	32
	5.9.2	Wash system ..............................................................................................................................	32
	5.9.3	Water saving ..............................................................................................................................	33
	5.9.4	Loading slide racks ...................................................................................................................	33
	5.9.5 Unloading racks from the exit drawer....................................................................................		34
	5.9.6 Unloading racks from other stations ......................................................................................		34
	5.9.7	Interrupting staining ..................................................................................................................	35
	5.9.8	Aborting a rack ..........................................................................................................................	36
6.	Cleaning and Maintenance ........................................................................................		37
	6.1	Cleaning the instrument ...........................................................................................................	37
	6.1.1	Wash containers .......................................................................................................................	37
	6.1.2	Reagent containers ...................................................................................................................	37
	6.1.3	Slide racks ..................................................................................................................................	37
	6.1.4	Oven .............................................................................................................................................	37
7.	Trouble shooting ...........................................................................................................		38
	7.1	Instrument failures ....................................................................................................................	39
	7.2	Information and warnings ........................................................................................................	40
	7.2.1	During staining ...........................................................................................................................	40
	7.2.2	During editing programs ...........................................................................................................	41
	7.2.3	During SetUp ..............................................................................................................................	42
8.	Warranty and service ..................................................................................................		43
	APPENDIX 1 ...................................................................................................................		44
	User-adjustable parameters ...............................................................................................................		44
	APPENDIX 2 ...................................................................................................................		45
	Consumables and accessories ...........................................................................................................		45
	APPENDIX 3 ...................................................................................................................		46
	Compatible staining programs ............................................................................................................		46
Glossary		....................................................................................................................................	48

Leica AutoStainer XL – Automated slide stainer

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1.Important information

The instruction manual for the Leica AutoStainer XL includes chapters dealing with the following subjects:

Chapter 1 Manual structure including:

•Table of contents

•Important information on this handbook.

Chapter 2

Safety

• Read this chapter before you attempt to operate the

instrument!

Chapter 3	Instrument features
•	General description
•	Technical data
Chapter 4	Installation
•	Site requirements
•	Installation
Chapter 5	Operation
•	Control elements
•	Menu map
Chapter 6	Trouble shooting
Chapter 7	Cleaning and maintenance
Chapter 8	Warranty and service
Appendix 1	User-adjustable parameters
Appendix 2	Consumables
	and accessories
Appendix 3	Compatible staining programs
Glossary

1.1Symbols occurring in the text and their meaning

Warnings and cautions appear in a grey box and are marked by a

warning triangle .

Notes,

i. e. important information for the user appear in a grey box and are

marked by an information sign

.

(5)Numbers in parentheses refer to

	(Fig. 5)	positions in the illustrations or to
		the illustrations themselves.

Instrument type:

All information in this instruction manual applies only to the instrument type indicated on the title page.

A nameplate with the instrument serial number is fixed on the back of the instrument.

Required information:

For all inquiries it is important to state the following:

•instrument type

•serial number.

6	Instruction manual V 2.1 – 10/2002

1. Important information

General

This instruction manual includes important information related to the operating safety and maintenance of the instrument.

The instruction manual is an important part of the product. It must be read carefully and completely before setup and first use of the instrument and must be kept near the instrument at all times.

If additional requirements on accident prevention and environmental protection exist in the country of operation, this instruction manual must be supplemented by appropriate instructions to ensure compliance with such requirements.

Be sure to read and comply with the safety instructions, warnings and cautions in chapter 2, even if you are already familiar with the operation and use of other Leica products.

1.2Designated use / improper use of the instrument

-The AutoStainer XL has been designed for staining applications in medicine, biology and industry.

-The instrument may be operated only according to the instructions contained in this manual.

-Any other use is considered an improper use of the product.

1.3Qualification of personnel

-The AutoStainer XL may be operated only by trained laboratory personnel.

-All laboratory personnel designated to operate the AutoStainer XL must read this instruction manual carefully and must be familiar with all technical features of the instrument before attempting to operate the AutoStainer XL.

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2.Safety

2.1Safety regulations

This instrument was built and tested in accordance with the safety regulations for electrical measuring, control, regulating and laboratory devices specified below

•DIN EN 292,

•DIN EN 61010-1,

•EN 50082-1,

•EN 55011,

•IEC 1000-4

as well as in accordance with the international quality standard

• DIN ISO 9001.

In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this instruction manual.

8	Instruction manual V 2.1 – 10/2002

3. Instrument features

The AutoStainer XL is the result of an extensive research program to provide an innovative stainer which meets the quality requirements of the modern laboratory, as well as:

•high throughput,

•flexibility,

•safety.

The AutoStainer XL achieves its high throughput by means of an innovative slide rack transfer mechanism which allows continuous loading of up 11 racks of 30 slides each.

The flexibility of AutoStainer XL also permits simultaneous processing of slide racks according to different staining protocols so that Papanicolau and Hematoxylin/Eosin staining can be conveniently performed at the same time, without reprogramming or reagent changes.

The AutoStainer XL incorporates all of the features which ensure high flexibility, convenience and above all, quality staining. A fan-forced oven is available to quickly dry slides and optimized wash stations result in rapid removal of excess reagent. The minimum-carryover design of the slide racks ensures that there are no drips and reagent life is extended.

The AutoStainer XL is safe to use and has an integral fume control system. Rack loading and unloading is achieved by a unique two-drawer system which means virtually no exposure to fumes.

The outstanding flexibility, throughput and quality-staining capability of AutoStainer XL has set a new standard in staining excellence.

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3.Instrument features

3.1Overview – instrument

1

20

	2
	19
	3
	18
	4
17	5
	6
16
						7
	11						8
15	9
	14

13

10

Abb. 1

12

10	Instruction manual V 2.1 – 10/2002

3.Instrument features

27

26

25

35

30

24

Abb. 2

Front view

1.Transfer mechanism

2.Wash stations

3.Oven

4.Program pad

5.Program pad recess

6.ON/STOP switch

7.Display screen

8.Keypad

9.LED indicators

10.Slide rack

11.Lid

12.Reagent container

13.Load drawer

14.Load LED and key

15.Exit drawer

16.Slotted lid

17.Exit LED and key

18.Container map on fume filter cover

19.Lid support

20.Lid

21

31

32

23

33

34

28

22

Rear panel

21.Water inlet

22.Drain outlet

23.Serial port

24.Power supply

25.Oven voltage selector

26.Mains switch (ON/OFF)

27.Mains outlet

28.Feet, adjustable

29.Power jumper cable

30.Power supply inlet

31.Rating and serial number plate

32.Remote alarm socket, 50 V 1 A max

33.Accessory port

34.Exhaust air duct

35.Power supply outlet

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3.Instrument features

3.2Standard delivery - packing list

Standard delivery:

1Leica ST5010 basic instrument (100-120 V/50-60 Hz)

1accessories kit (0456 35660) consisting of:

	- 22 reagent vessels with lids .....................................................................................................	0475 33659
	- 5 wash vessels ...........................................................................................................................	0456 35268
	- 5 slide racks, metal....................................................................................................................	0456 33919
	- 2 lids with slots for reagent vessels .......................................................................................	0475 34486
	- 1 jumper cable - supply system ...............................................................................................	0411 34604
	- 1 remote alarm connector ........................................................................................................	6844 01005
	- 1 hose clamp ..............................................................................................................................	0422 31972
	- 1 angular connecting nozzle for hose ....................................................................................	0475 33669
	- 1 V filter 3/4 .................................................................................................................................	0456 36101
1	activated carbon filter .................................................................................................................	0474 32273
1	filter cover .....................................................................................................................................	0456 35240
1	drip tray for paraffin (heating) (inside the instrument) ...........................................................	0456 35216
1	dye log block (attached to the instrument) ..............................................................................	0456 35459
1	water supply hose with seal ......................................................................................................	0474 32325
1	drain hose .....................................................................................................................................	0475 35748
1	power cable USA-C-J .................................................................................................................	0411 13559
1	operating manual Leica AutostainerXL G/E/F/S ....................................................................	0456 25634
	- 1 reference manual as appendix, English only (dye logs, etc.)

12	Instruction manual V 2.1 – 10/2002

	3. Instrument features
3.3 Technical Data
Specimen slide throughput: ..............................................................	at least 200 specimen slides per hour
	(depending on the selected program - up to 600 slides per hour)
Loading capacity: .........................................................................................................................	11 slide racks
Slide rack capacity: .............................................................................................................	30 specimen slides
Total number of stations: .................................................................................................................................	26
Total number of reagent stations: ....................................................................................................	at least 18
Reagent container volume: ......................................................................................................................	450 ml
Number of wash stations: .......................................................................................................	................. max. 5
Oven: .....................................................................................................................................................................	1
Oven chamber temperature: ....................................................................................	ambient or 30°C to 65 °C
Incubation time setting: ...............................................................................	from 0 sec. up to 99 min., 59 sec
Load/unload stations: ...............................................................................................................................	1 each
Permanent memory capacity: ..................................................	15 programs, up to 25 program steps each
Operating temperature range: ....................................................................................................	15 °C to 35 °C
Relative humidity: .................................................................................................	max. 80 %, non-condensing
Dimensions (W x D x H): .............................................................................................	109 cm x 67 cm x 51 cm
Weight: ..........................................................................................................................................................	65 kg
Voltages: ..................................................................................................................	110 V - 120 V, 50 Hz - 60 Hz
	230 V - 240 V, 50 Hz - 60 Hz

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4.Installation

Instructions on how to install the instrument are provided in this chapter. A diagram and description of components is also given. Finally, the procedure for replacement of the fume filter is outlined.

4.1Site requirements

The AutoStainer XL requires a solid bench of dimensions 1.090 mm long and 670 mm deep. The instrument must be located within 3 meters of a tap and drain.

The power requirements for the AutoStainer XL are:

8 amps: at 110 volts

4 amps: at 240 volts

The voltage selector and other internal components are set by the manufacturer to suit the country of sale.

The voltage selector setting must not be altered by the user.

The AutoStainer XL requires connection to a laboratory water tap with mains pressure fitting.

4.2 Connection

4.2.1 Power

•Connect the power cord to the mains outlet ((28) page 11).

•Connect the power jumper cable to the power supply outlet ((30) page 11) and to the power supply inlet ((32) page 11).

Refer to Figure 10 on page 11.

14	Instruction manual V 2.1 – 10/2002

4.Installation

How to switch on:

1.Connect the power cord to the mains power socket and, if applicable, switch power ON at the mains socket.

2.Set the ON/STOP switch at the side of the unit to STOP.

3.Set the ON/OFF switch at the rear of the unit to ON.

4.Set the ON/STOP switch to ON.

The instrument will then sound 3 short beeps and the Main Menu will be displayed.

When the instrument is not in use set the ON/STOP switch at the side to STOP.

The ON/OFF switch at the rear of the instrument should be left ON.

The instrument must never be operated without the power jumper cable.

The instrument must be connected to an earthed mains power outlet socket only.

4.2.2 Water supply

Connect the water hose to the water inlet at the rear of the unit. Screw the other end of the hose to the cold water tap. The hose has a 3/4 inch BSP fitting. Slowly turn the tap on fully.

Ensure that the water filter is present when fitting the water inlet hose. Failure to do so may result in leakage of water.

Drain hose

Connect the drain hose to the drain outlet on the rear of the unit.

Leica AutoStainer XL – Automated slide stainer

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