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INSTRUCTION MANUAL
MCO-20AIC
CO2 Incubator
Current Revision December 02
1
Note:
1.No part of this manual may be reproduced in any form with the expressed written permission of SANYO.
2.The contents of this manual are subject to change without notice.
3.Please contact SANYO if any point in this manual is unclear or if there are any inaccuracies.
SANYO Electric Co., Ltd. All rights reserved. |
Printed in Japan. |
CONTENTS
PRECAUTIONS FOR SAFE OPERATION |
P. 2 |
CAUTIOINS FOR USAGE |
P. 6 |
INCUBATOR COMPONENTS |
P. 8 |
INSTALLATION |
P. 11 |
BEFORE COMMENCING OPERATION |
P. 13 |
OPERATING INSTRUCTIONS |
P. 16 |
ALARM & SAFETY FUNCTIONS |
P. 20 |
ROUTINE MAINTENANCE |
P. 22 |
CALIBRATION |
P. 26 |
TROUBLE SHOOTING |
P. 27 |
ENVIRONMENTAL CONDITIONS |
P. 28 |
DISPOSAL OF UNIT |
P. 28 |
STACKED MODULE |
P. 29 |
SPECIFICATIONS |
P. 30 |
PERFORMANCE |
P. 31 |
SAFETY CHECK SHEET |
P. 32 |
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PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with this manual as it contains important safety advice.
Items and procedures are described so that you can use this unit correctly and safely. If the precautions advised are followed, this will prevent possible injury to the user and any other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property.
Symbol shows;
this symbol means caution.
this symbol means an action is prohibited.
this symbol means an instruction must be followed.
Be sure to keep this manual in a place accessible to users of this unit.
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PRECAUTIONS FOR SAFE OPERATION
WARNING
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to rain water.
Only qualified engineers or service personnel should install the unit. The installation by unqualified personnel may cause electric shock or fire.
Install the unit on a sturdy floor. If the floor is not strong enough or the installation site is not adequate, this may result in injury from the unit falling or tipping over.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
Never install the unit in a flammable or volatile location. This may cause explosion or fire.
Never install the unit where acid or corrosive gases are present as current leakage or electric shock may result due to corrosion.
Use a dedicated power source as indicated on the rating label attached to the unit.
Remove dust from the power supply plug before inserting in a power source. A dusty plug or improper insertion may pose a hazard.
Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is not grounded, it will be necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such grounding may cause electric shock in the case of an incomplete circuit.
Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are connected correctly and are not liable to become disconnected. Ensure that the gas pressure is set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure may result in leakage of CO2 gas. Elevated level of CO2 gas can be hazardous to health and may lead to asphyxiation and risk of death.
Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet for inner air circulation. This may cause electric shock or injury by accidental contact with moving parts.
Never store volatile or flammable substances in this unit. This may cause explosion or fire.
4
PRECAUTIONS FOR SAFE OPERATION
WARNING
As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity of the equipment. It is important that you assess the work site to ensure there is suitable and sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a safe environment must be considered. These may include atmosphere monitoring and warning devices.
Ventilate a room air occasionally when using CO2 gas for control. The gas density will increase in an enclosed small room and high level of gas density is harmful for human. In addition, avoid inhaling the chamber air directly when opening the door if CO2 gas is used.
Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densité CO2 de l’air peut s’élever et peut être nocif aux humains. Évitez d’aspirer l’air provenant de l’intérieur de l’appareil quand vous ouverz la porte.
Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use may cause bad effect on your health or environment.
Never expose the eyes directly to the UV light as the UV light is harmful.
Disconnect the power supply to the unit prior to any repair or maintenance of the unit in order to 
prevent electric shock or injury.
Ensure you do not inhale or consume medication or aerosols from around the unit at the time of maintenance. These may be harmful to your health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never disassemble, repair, or modify the unit yourself. Any such work carried out by an unauthorized person may result in fire or injury due to a malfunction.
Disconnect the power supply plug if there is something wrong with the unit. Continued 
abnormal operation may cause electric shock or fire.
If the unit is to be stored unused in an unsupervised area for an extended period, ensure that children do not have access and that doors cannot be closed completely.
The disposal of the unit should be accomplished by appropriate personnel. Remove doors to prevent accidents such as suffocation.
Prepare a safety check sheet when you request any repair or maintenance for the safety of service personnel.
5
PRECAUTIONS FOR SAFE OPERATION
CAUTION
Select a level and sturdy floor for installation. This precaution will prevent the unit from tipping. Improper installation may result in water spillage or injury from the unit tipping over.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use of any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Fix the shelves securely. Incomplete installation may cause injury or damage.
When removing the plug from the power supply outlet, grip the power supply plug, not the cord. Pulling the cord may result in electric shock or fire by short circuit.
Never damage or break the power supply plug or cord. Do not use the supply plug if its cord is loose. This may cause fire or electric shock.
Do not touch any electrical parts such as the power supply plug or any switches with a wet hand. This may cause electric shock.
Do not put a container with water or heavy articles on the unit. It may cause injury if the articles 
fall. Current leakage or electric shock may be resulted form the deterioration of insulation by spilled
water.
Do not climb onto the unit or do not put articles on the unit. This may cause injury by tipping or damage to the unit.
Never lean or press on the glass. Intentional force may cause injury if the glass breaks.
Do not lean on the door. This may cause injury, current leakage, or electric shock if the unit tips over or door drops out.
Disconnect the power supply plug before moving the unit. Take care not to damage the power cord. A damaged cord may cause electric shock or fire.
Empty the humidifying pan completely before moving the unit. Spilled or splashed water may cause current leakage or electric shock.
Be careful not to tip over the unit during movement to prevent damage or injury.
Disconnect the power plug when the unit is not used for long periods. |
Do not put the packing plastic bag within reach of children as suffocation may result
6
CAUTIONS FOR USAGE
1. Install in proper environment
With an automatic calibrating function, the CO2 sensor is calibrated according to an ambient air gas. Place the unit in proper environment.
2. 5oC higher than the ambient temperature
The chamber temperature must be at least 5oC higher than the ambient temperature. For example, the chamber temperature is set to 37oC, the ambient temperature must be less than 32oC. Keep the ambient temperature in adequate range.
3. Do not subject to direct air flow
Do not allow the air for air conditioning to hit the unit or door directly. Direct hit may cause condensation or contamination.
4. Always keep the chamber clean
The Petri dishes or bottles for culturing may cause contamination in the chamber. Clean the containers before storing them in the chamber.
5. Allow adequate space between the cultures
When storing cultures in the chamber, keep the Petri dishes or bottles containing the cultures sufficiently apart from each other to allow adequate air circulation. Inadequate space may result in uneven temperature distribution and CO2 concentration in the chamber.
6. Always shut the inner door
Shut the inner door completely, and then shut the outer door. If the inner door is not closed completely, even if the outer door is closed, the unit will fail to exhibit its maximum performance. And close the doors gently. Rude closing may cause spillage of medium, incomplete closing, or damage of gasket. Before opening the inner door, check that the UV light is OFF.
7. Keep the inside panels dry
To protect the inside of the unit from contamination, the inside panels should always be kept dry. If water is spilled from a humidifying pan or if the door is kept open for a long period, condensation will form on the panels, allowing germs to breed. In such a case, wipe away the water with a dry sterile gauze. Particularly, if the medium is spilled, wipe it up immediately and sterilize the area.
8. Fill the humidifying pan with sterile distilled water
Always use sterile distilled water to fill the pan. The RH PAN light on the control panel flashes when the water level is low. Refill the sterile distilled water to the pan when the RH PAN light blinks. Note that when low temperature water is poured, the chamber temperature drops significantly. Clean the pan once a month.
9. Humidifying pan and pan cover
The humidifying pan and pan cover prevent the UV light from releasing. Make sure to install them even if you do not need humidity. Install the pan cover properly since it affect the temperature distribution and humidity recovery in the chamber.
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CAUTIONS FOR USAGE
10. Stored materials
Never place the acid or alkaline materials or materials that release corrosive gas in the chamber. Such materials can cause failure resulting from the discoloration or corrosion.
11. Alarm
Always investigate the cause and fix the alarm condition immediately when the alarm is activated. Refer to page 20 for the alarm.
12. Never use CAL key
Never use CAL key during normal operation because the calibration mode is start by pressing this key. Wrong operation of this key will give an adverse effect on the basic performance of the unit. If you press this key accidentally, never press any keys on the control panel for 90 seconds, which returns the unit to the display mode automatically.
13. UV lamp
The UV lamp is OFF when the outer door is opened since it is related to the outer door switch. Check the UV lamp is ON with the inner door closed once a month by activating the outer door switch. If the lamp is not ON, the lamp needs to be replaced. Contact Sanyo sales representative or agent.
The UV lamp is located in the duct. Take care not to damage the lamp at the time of installation/removal of attachments or humidifying pan.
Note: The UV light is harmful for the eyes. Never turn on the UV lamp with the inner door opened.
14. No use for long period
When the unit is not used for long period, dispose the water in the humidifying pan and remove the humidity in the chamber completely. Check that the chamber is enough dry before closing the doors.
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INCUBATOR COMPONENTS
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9 (inside) |
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13, 15, 16 (inside)
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19 |
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20
Rear right side |
Rear left side |
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INCUBATOR COMPONENTS
1.Outer door: Sticks to frame with magnetic packing. Door heater is installed in the door panel. The heater eliminates moisture on the inner door. The door opening is reversible. Contact Sanyo representative or agent to change the door hinge side.
2.Inner door: Made of tempered glass. Avoid the excessive impact on the glass.
3.Leveling leg: Used for leveling the unit.
4.Tray: Can be pulled toward you.
5.Tray support: Can be removed by lifting the front side and pulling toward you.
6.Side support: Right and left side supports are removal for disinfection. See page 23 and 24.
7.Top duct: Inlet of circulating air. Removable.
8.Depth duct: Flow path for circulating air. Removable.
9.Fan (inside the depth duct): Made from polypropylene resin. Can be disinfected by an autoclave.
10.Sample air outlet: This also functions as an internal gas outlet, so do not cap it.
11.Connecting port for CO2 gas pipe (back side): When an optional component MCO-20GC is installed, both A and B are available. In the case of no MCO-20GC, only A is available. Refer to page
14for gas cylinder connection. Ensure that the gas pressure is set at 0.03MPaG (0.3kgf/cm2G, 4.3psiG).
12.Door switch: Detects the door opening/closing and stops the circulating fan and electromagnetic valve for CO2 when door is open. And also turns off the UV lamp by door opening.
13.Humidifying pan: Use the sterile distilled water to fill the pan. Install the pan properly so that it can be covered with the pan cover.
14.Humidifying pan cover: Prevents the UV light from exposing to the chamber. When filling the pan, lift the front side and take out the pan. See page 23 for the details.
15.UV lamp: A UV lamp that never generates ozone. Never look at the UV light directly. For the replacement, contact Sanyo representative or agent.
16.Water level sensor for humidifying pan: Located at the depth of the chamber and detects the water level in the humidifying pan. Never add an excessive force on the sensor at the time of maintenance.
17.Remote alarm terminal: Refer to page 21.
18.Access port: When no use, cap with a rubber cap on both outside and inside.
19.Power switch: Main switch of the unit. Also has a function as a over-current breaker (15A).
20.Glow lamp: For UV lamp (model; FG-7P)
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INCUBATOR COMPONENTS
Control panel and keypad
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1.Digital temperature indicator (TEMPERATURE oC): Normally, this indicator shows the chamber temperature. In the setting mode, it shows the set value of the chamber temperature. If the self diagnostic function detects any abnormality, an error code will be displayed.
2.Heater lamp (HEAT): This lamp lights when the heater is energized.
3.UV indicator (UV): This lamp lights when the UV lamp is ON.
4.Door lamp (DOOR): This lamp lights when the outer door is open.
5.Water level alarm lamp (RH PAN): This lamp flashes when the water in the humidifying pan is less than about 1 liter.
6.Upper limit regulator: This regulator is used to set the upper limit temperature.
7.Over heat lamp (OVER HEAT): This lamp lights when the chamber temperature reaches the upper limit set value.
8.CO2 inject lamp (INJECT): This lamp lights when CO2 gas is being supplied.
9. Digital CO2 density indicator (CO2 %): Normally, this indicator shows the CO2 concentration in the chamber. In the setting mode, it indicates the set value of the CO2 concentration.
10.Set key (SET): Pressing this key to enter the setting mode, and the digits that can be set flash.
11.Calibration key (CAL): By pressing this key for about 5 seconds, the unit enters a function mode. Used for change the UV lamp ON period.
12.Alarm buzzer stop key (BUZZER): Press this key to silence the buzzer when the alarm operates and the buzzer sounds. The remote alarm also stops by this key. Press it once again to reactivate the buzzer.
13.CO2 gas supply line indicator (A/B): The lamp of supply line currently used brightens provided that MCO-20GC is installed. This will flash when a gas cylinder is empty.
14.CO2 gas supply line switching key : This key to select CO2 gas supply line is available only when
an automatic cylinder switcher MCO-20GC (option) is installed. When one CO2 gas cylinder is empty, the CO2 is supplied by the other cylinder automatically. But if necessary, by pressing this key, the supply line can be switched between A and B manually.
15. Enter key (ENT): Pressing this key memorizes the set value in the controller.
16. Numerical value shift key ( ): Pressing this key in the setting mode causes the numerical value to shift. In key lock mode, pressing this key makes key lock ON or OFF.
17. Digit shift key (
): Pressing this key in the setting mode causes the changeable digit to shift.
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