FemtoJet 4x
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FemtoJet
®
4i
Operating manual
5252 900.014-00/012014
Copyright © 2014 Eppendorf AG, Hamburg. All rights reserved, including graphics and
images. No part of this publication may be reproduced without the prior permission of the
copyright owner.
Eppendorf
®
and the Eppendorf logo are registered trademarks of Eppendorf AG,
Hamburg, Germany.
FemtoJet
®
, Femtotips
®
, TransferMan
®
and InjectMan
®
are registered trademarks of
Eppendorf AG, Hamburg, Germany.
Registered trademarks and protected trademarks are not marked in all cases with
®
or ™
in this manual.
Table of contents
FemtoJet
®
4i
English (EN)
3
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.1.1 Delivery package – Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3 Exemplary set-up of a microinjection system . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.4 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.4.1 Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.5 Name plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.6 Control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.7 Rotary knobs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.8 Foot control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.9 Hand control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.10 Pressure parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.10.1 Compensation pressure p
c
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.10.2 Injection pressure p
i
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.10.3 Injection time t
i
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.10.4 Rinsing pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.10.5 Operating pressure p
w
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.11 Self-calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.12 Universal capillary holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.3 Warning signs on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.4 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.5 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.1.1 Complaints about damages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.1.2 Incomplete delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.3 Connecting the Microinjector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Table of con tents
FemtoJet
®
4i
English (EN)
4
4.4 Connecting an external device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.4.1 Connecting a micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.4.2 Connecting the PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.4.3 Connecting two devices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.5 Connecting accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.5.1 Connecting a foot control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.5.2 Connecting a hand control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.1 Main screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2 Main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.3 Navigating the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.3.1 Selecting the menu and navigating . . . . . . . . . . . . . . . . . . . . . . . . 24
5.3.2 Exiting the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.3.3 Selecting parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.3.4 Changing a parameter value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.1 Switching on or off the Microinjector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.1.1 Switching on the Microinjector . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.1.2 Switching off the Microinjector . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.2 Switching on or off the standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.2.1 Switching on the standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . 26
6.2.2 Switching off the standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.3 Determining the injection parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.3.1 Filling the capillaries with fluorescence dye . . . . . . . . . . . . . . . . . 27
6.3.2 Carrying out a test injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
6.3.3 Possible sources of error - cell inflates or bursts . . . . . . . . . . . . . 28
6.3.4 Possible sources of error - capillary is clogged. . . . . . . . . . . . . . . 28
6.3.5 Possible sources of error - liquid is not being injected . . . . . . . . . 28
6.3.6 Possible sources of error - capillary does not reach the cell. . . . . 28
6.3.7 Result – the injection parameters have been determined . . . . . . . 28
6.4 Setting the compensation pressure p
c
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.5 Setting the injection pressure p
i
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.6 Setting the injection time t
i
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.7 Setting the injection mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.7.1 Setting the automatic injection mode . . . . . . . . . . . . . . . . . . . . . . 30
6.7.2 Setting the manual injection mode . . . . . . . . . . . . . . . . . . . . . . . . 30
6.8 Injecting liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.8.1 Automatically injecting liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.8.2 Manually injecting liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.9 Replacing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.10 Rinsing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.11 Setting the injection counter to zero. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Table of contents
FemtoJet
®
4i
English (EN)
5
6.12 Calling up saved injection parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.12.1 Calling up saved injection parameters . . . . . . . . . . . . . . . . . . . . . 32
6.13 Saving or changing the injection parameters . . . . . . . . . . . . . . . . . . . . . . . . 32
6.13.1 Saving injection parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.13.2 Changing the saved injection parameters . . . . . . . . . . . . . . . . . . . 33
6.14 Adjusting the device settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
6.14.1 Change capillary function – Changing the capillary . . . . . . . . . . . 33
6.14.2 Pressure unit function – Selecting the pressure unit . . . . . . . . . . 33
6.14.3 Beeper function – Switching on/off the signal tone . . . . . . . . . . . 33
6.14.4 Contrast function – Setting the display contrast . . . . . . . . . . . . . . 33
6.14.5 Illumination function – Switching on/off the
display illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6.14.6 Continuous flow function – Setting the constant
operating pressure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6.14.7 Drain pressure supply function – Draining the
pressure reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6.15 Inserting the capillary into the capillary holder . . . . . . . . . . . . . . . . . . . . . . 35
6.16 Inserting Femtotips into the capillary holder . . . . . . . . . . . . . . . . . . . . . . . . 35
7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7.2.1 Error 01 – 10 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7.2.2 Error 11 – 18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.2.3 Error 19 – 38 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7.2.4 Error 39 – 40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
8.1 Replacing the sealings in the grip head . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
8.1.1 Removing the sealing washer and o-rings . . . . . . . . . . . . . . . . . . 41
8.1.2 Inserting the o-rings and the sealing washer . . . . . . . . . . . . . . . . 42
8.2 Replacing the sealing in the adapter for Femtotips . . . . . . . . . . . . . . . . . . . 42
8.2.1 Replacing the o-ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
8.3 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
8.4 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
8.5 Hints with regard to service intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
8.6 Service and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
8.7 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Table of con tents
FemtoJet
®
4i
English (EN)
6
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.1 Mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.3 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.4 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.4.1 USB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.4.2 RS 232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
9.5 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
10.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
10.3 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
10.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
11 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
11.1 FemtoJet 4i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
11.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
11.3 Universal capillary holder and accessories. . . . . . . . . . . . . . . . . . . . . . . . . . 50
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Operating instructions
FemtoJet
®
4i
English (EN)
7
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Also
observe the instructions for use of the accessories.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
You will find the current version of the operating manual for all available languages on
our webpage under www.eppendorf.com
.
1.2 Danger symbols and danger levels
The safety instructions of this operating manual indicate the following danger symbols
and danger levels:
1.2.1 Danger symbols
1.2.2 Danger levels
1.3 Symbols used
Electric shock Hazard point
Cuts Material damage
Biohazard
DANGER Will lead to severe injuries or death.
WAR NIN G May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
Depiction Meaning
1.
2.
Actions in the specified order
Actions without a specified order
• List
Direction of movement
Text Display text or software text
Additional information
Product description
FemtoJet
®
4i
English (EN)
8
2 Product description
2.1 Delivery package
2.1.1 Delivery package – Accessories
2.2 Features
The Microinjector can be used to inject very small amounts of fluid into cells. The
parameters for pressure and time are set on the device and controlled by the software. It
is possible to trigger the injection on the Microinjector or on a connected
micromanipulator by Eppendorf. The semiautomatic injection movement is controlled by
the Microinjector or the micromanipulator. The required pressure is generated by a
soundproof internal compressor.
Number Description
1 FemtoJet 4i
1Mains/power cord
1Injection tube
1 Operating manual
Number Description
1 Universal capillary holder
1 Grip head 0 for capillary (outer diameter 1.0 mm to 1.1 mm)
1 Adapter for Femtotips
1 Foot control
Product description
FemtoJet
®
4i
English (EN)
9
2.3 Exemplary set-up of a microinjection system
Abb. 2-1:Microinjection system with FemtoJet 4i
Fig. 2-1: Microinjection system with FemtoJet 4i
1 Micromanipulator InjectMan 4
2 Universal capillary holder with
capillary
3Inverse microscope
4 Injection tube
5Control board InjectMan 4
6 Microinjector FemtoJet 4i
FemtoJet
inject
prog 1
prog 2
count
clean
menu
enter
auto
man
2
1
3
6
5
4
Product description
FemtoJet
®
4i
English (EN)
10
2.4 Main illustration
Abb. 2-2:Front and rear side
Fig. 2-2: Front and rear side
1Display
2 Control panel
3 Port for injection tube
4 Rotary knobs for injection parameters
5 Name plate
Lower side of device
6Interfaces
7Venting
Lower side of device
menu
enter
auto
man
inject
clean
count
prog 1
prog 2
FemtoJet
1
2
4
5 763
Product description
FemtoJet
®
4i
English (EN)
11
2.4.1 Interfaces
Abb. 2-3:Interfaces
Fig. 2-3: Interfaces
1 Mains/power connection
2Mains/power switch On/Off
3Micro fuse
4 Port for micromanipulator or
computer
5 Connection for foot or hand control
6 Service connection
MANIPULATOR
FOOT SWITCH
SERVICE
4
5
6
213
Product description
FemtoJet
®
4i
English (EN)
12
2.5 Name plate
Abb. 2-4:Name plate
Fig. 2-4: Name plate
1 Manufacturer
2Product number
3Voltage
4Micro fuse
5Frequency
6Labelings
7Address of manufacturer
8 Serial number
9Output
10 Current consumption
1
2
3
4
5
6
7
8
10
9
Product description
FemtoJet
®
4i
English (EN)
13
2.6 Control panel
The keys of the control panel are used to switch on the Microinjector, to perform
functions, to select programs and to navigate through the menu.
Abb. 2-5:Control panel
Fig. 2-5: Control panel
1 standby key
Activate/deactivate standby mode
2 menu enter key
Open the menu
3Arrow key
Navigate the menu
4 auto man key
Toggle between automatic and manual
injection
5 prog 1 key
Select or save parameter set 1
6 prog 2 key
Select or save parameter set 2
7 inject key
Perform an injection
8 clean key
Clean the capillary
9 count key
Set the counter to zero
countcountcount
clean
prog 1
5 6
7
8
9
4
3
2
1
Product description
FemtoJet
®
4i
English (EN)
14
2.7 Rotary knobs
The rotary knobs are used to set the injection parameters as injection time, injection
pressure and compensation pressure.
Abb. 2-6:Rotary knobs
Fig. 2-6: Rotary knobs
1 Rotary knob
Set the injection pressure p
i
2 Rotary knob
Set the injection time t
i
3Rotary knob
Set the compensation pressure p
c
enter
countcountcountcount
cleanclean
auto
man
321
Product description
FemtoJet
®
4i
English (EN)
15
2.8 Foot control
You can connect the foot control to the Microinjector.
The foot control corresponds to the following key on the Microinjector:
• Foot control – inject key
Abb. 2-7:Foot control with plug
Fig. 2-7: Foot control with plug
2.9 Hand control
You can connect the hand control to the Microinjector.
The hand controls correspond to the following keys on the Microinjector:
• Left hand control – inject key
• Right hand control – clean key
2.10 Pressure parameters
The parameters are used to define the pressure and time for the injection and the
cleaning of the capillary.
The pressure parameters include the following parameters:
• Compensation pressure – p
c
• Injection pressure – p
i
• Injection time – t
i
• Operating pressure
• Rinsing pressure
The inject key remains active when the foot control is connected.
The hand control is not included in the delivery package and must be ordered
separately.
The inject and clean keys remain active when the hand control is connected.
Product description
FemtoJet
®
4i
English (EN)
16
2.10.1 Compensation pressure p
c
The compensation pressure prevents the liquid from rising from the Petri dish into the
capillary due to the capillary action. Due to the compensation pressure, some liquid will
leak constantly from the capillary tip. This prevents the injection material from clumping
together.
Abb. 2-8:Pressure ratios in the capillary
Fig. 2-8: Pressure ratios in the capillary
2.10.2 Injection pressure p
i
The injection pressure defines the pressure used for injecting liquid into the cell. During
the injection process, the injection pressure is applied as long as the injection time is
running. To inject liquid into a cell, the injection pressure must be higher than the inside
pressure of the cell.
2.10.3 Injection time t
i
The injection time defines the time period for injecting the liquid. The start of the
injection time depends on the presettings of the micromanipulator. The injection time
begins either when triggering the injection function or when reaching the lower safety
limit. The injection pressure is applied as long as the injection time is running.
1Capillary
2 Liquid with injection material
3 Compensation pressure p
c
4Hydrostatic pressure
5 Capillary action
3
4
5
1
2
Product description
FemtoJet
®
4i
English (EN)
17
2.10.4 Rinsing pressure
The rinsing pressure is used to clean the capillary.
2.10.5 Operating pressure p
w
The operating pressure subsumes the injection pressure, compensation pressure and
rinsing pressure.
2.11 Self-calibration
The Microinjector performs a calibration every two hours. During the calibration, the
ventilation valve opens and the collected condensation water is discharged.
2.12 Universal capillary holder
You can insert a capillary or a Femtotip into the universal capillary holder.
Abb. 2-9:Universal capillary holder
Fig. 2-9: Universal capillary holder
1Capillary
2Grip head
3Knurled screw
4 Universal capillary holder
5 Connection for injection tube
6Femtotips
7 Adapter for Femtotips
1 2 3 4
6 7
5
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