Eppendorf 5070 User Manual

0 (0)

070neN)manual

www.eppendorf.com/myeppendorf

epMotion® 5070

Hardware manual

Trademarks

Eppendorf®, the Epppendorf logo, epMotion®, Eppendorf Tubes ®, epT.I.P.S.® and are registered trademarks of Eppendorf AG.

LightCycler® and MagNA Pure® are registered trademarks of Roche Diagnostics.

epMotion optical sensor U.S. Pat. No. 6,819,437

Registered trademarks are not marked in all cases with ® or ™ in this manual.

The software of the device (firmware) contains open source software. License information is available on request from Eppendorf AG.

5070 900.850 -00/062013

Table of contents epMotion® 5070 3

English (EN)

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.6 Revision history. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.1.1 epMotion 5070 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.1.2 epMotion 5070f . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 2.1.3 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 2.1.4 EasyCon. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 2.3.1 Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 2.3.2 Cleanbench . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3.1.1 Intended use of the epMotion 5070 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3.1.2 Intended use of the epMotion 5070f. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 3.5 Hazard symbols and safety devices on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 4.2 Installing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5 Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

5.1 Worktable for epMotion 5070 and 5070f . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

5.2 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.3 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.3.1 Dispensing Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Table of contents

4epMotion® 5070 English (EN)

6 Dispensing liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.1 Basic procedure for dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 6.1.1 Operating principle of dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 6.1.2 Liquid aspiration up to the remaining volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 6.1.3 Bottom tolerance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 6.1.4 Transporting and dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

6.2 Dispensing modes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 6.2.1 Dispensing mode: Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 6.2.2 Dispensing mode: Multidispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 6.2.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6.3 Special features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 6.3.1 Liquid aspiration from bottom of vessel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 6.3.2 Liquid aspiration from high vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 6.3.3 Liquid dispensing at the height of the vessel edge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

7 Labware – vessels, plates and pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

7.1 Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 7.2 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 7.2.1 Deepwell plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 7.2.2 Microplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 7.2.3 PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 7.2.4 Tube plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

7.3 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 7.4 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 7.4.1 epT.I.P.S. Motion Racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 7.4.2 epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 7.4.3 Tip holder for epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 7.4.4 epT.I.P.S. Motion SafeRacks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

8 Labware accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

8.1 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 8.1.1 Racks for 24 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 8.1.2 Rack for 96 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 8.1.3 Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 8.1.4 Thermorack and Rack 0.5/1.5/2.0 mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 8.1.5 Reservoir Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 8.1.6 Reservoir Rack modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

8.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 8.2.1 Height adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 8.2.2 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 8.2.3 Thermoadapter LC samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 8.2.4 Thermoadapter Frosty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

8.3 Thermoblocks and Thermoadapters for PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 8.3.1 Equipping the thermoblock with 0.2 mL vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 8.4 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 8.4.1 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 8.4.2 LiquidWasteTub with lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Table of contents epMotion® 5070 5

English (EN)

9 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

9.1 First steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 9.1.1 Switching on the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 9.1.2 Using the EasyCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 9.1.3 Using the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

9.2 Loading the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 9.2.1 Display the loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 9.2.2 Positioning tools on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 9.2.3 Positioning the epT.I.P.S. Motion on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 9.2.4 Positioning the waste container on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 9.2.5 Positioning labware on the worktable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

9.3 Executing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 9.3.1 Performing a test run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 9.3.2 Starting the application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 9.3.3 Controlling the application run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

9.4 Completing your work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 9.4.1 Cleaning up the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 9.4.2 Switching off the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

10.1 Decontamination and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 10.1.1 Carrying out wipe decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72 10.1.2 Carrying out spray decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 10.1.3 Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

10.3 Servicing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 10.5 Carrying out firmware updates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 10.6 Checking the dispensing volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 10.7 Maintaining the dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

10.7.1 Exchanging the sealing rings on the eight-channel dispensing tool . . . . . . . . . . . . . . 76 10.8 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

11.1 Error search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79 11.2 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 11.2.1 Optical sensor errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 11.2.2 Dispensing errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 11.2.3 Software errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

12.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 12.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 12.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

Table of contents

6epMotion® 5070 English (EN)

13 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

13.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 13.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 13.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 13.4 Noise level. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 13.5 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 13.6 EasyCon. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 13.7 Additional specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

13.7.1 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 13.7.2 Carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 13.8 Dispensing tool errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 13.8.1 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 13.8.2 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

13.9 Test conditions for dispensing tool errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

14.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 14.2 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 14.3 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 14.4 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 14.5 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92 14.6 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 14.7 Thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 14.8 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

Operating instructions epMotion® 5070 7

English (EN)

1Operating instructions

1.1Using this manual

Your epMotion operating manual consists of hardware instructions and software instructions. Short instructions are available for optional software enhancements.

The operating manual is part of the product.

The current version of the operating manual can be found on our webpage: www.eppendorf.com.

Read the operating manual in full before using the device.

Store the operating manual at an easily accessible location.

The device may only be transferred with the operating manual.

If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.

1.2Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1	Danger symbols
	Biohazard	Explosion
	Electric shock	Heavy loads
	Cuts	Hot surface
	Hazard point	Material damage

1.2.2Danger levels

DANGER	Will lead to severe injuries or death.

WARNING	May lead to severe injuries or death.

CAUTION	May lead to light to moderate injuries.

NOTICE	May lead to material damage.

Operating instructions

8epMotion® 5070 English (EN)

1.3Symbols used

Depiction			Meaning

1.			Actions in the specified order
2.

			Actions without a specified order

•			List:

Text			Display or software texts

			Additional information
			Additional information

1.4Abbreviations used

DNA

Deoxyribonucleic acid (DNA)

DWP

Deepwell plate

epT.I.P.S.

eppendorf Totally Integrated Pipetting System

Liquid handling

MTP

Micro test plate

PCR

Polymerase chain reaction

PDF

Portable Document Format

USB

Universal Serial Bus

Operating instructions epMotion® 5070 9

English (EN)

1.5Glossary

Application

Programs for a specific application. An application includes the procedure and the equipping of the worktable.

Cleanbench

Class 2 safety cabinet for personal security and product protection. A laminar air flow prevents germs from entering the cabinet from the ambience. The air flow also prevents aerosols that contain microorganisms from escaping the cabinet.

Destination labware

Labware that liquid is dispensed into during the application. Destination labware refers to a plate or rack.

Destination position

Position that liquid is dispensed into during the application.

Destination vessel

The vessel into which liquid is dispensed during the application.

Dispensing tool

Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing tools are available for various volume ranges.

EasyCon

epMotion control panel. You can control your epMotion using the control panel and epBlue software.

epBlue

Software for creating and administering applications and labware. The epMotion is controlled using the software.

Eppendorf Quality

Eppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the requirements for standard products, e.g., precision, accuracy, wetting behavior and tightness.

epT.I.P.S.® Motion

epMotion pipette tips. Only epT.I.P.S.® Motion can be used on the epMotion for all features. epT.I.P.S.® Motions are available with or without a filter.

Operating instructions

10epMotion® 5070 English (EN)

Filling volume

Maximum liquid volume of a labware. The epMotion uses the gripper to transport the labware up to the filling volume. The epMotion aspirates liquid from the labware up to the filling volume. The filling volume is higher than the working volume.

Height adapter

Adapter for low labware. The height differences among the labware will be offset to decrease the tool holder paths, thereby decreasing the run time of the application as well.

Intermediate labware

Labware that is used to dispense and aspirate liquids during the application.

Intermediate position

Position that is used to dispense and aspirate liquids during the application.

Intermediate vessel

Vessel that is used to dispense and aspirate liquids during the application.

Labware

Racks, plates, tips, etc. which are placed on the worktable.

Location

Area on the worktable where the labware is placed.

PCR clean

PCR clean is an Eppendorf AG purity grade for consumables. PCR clean meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. PCR clean also meets the requirements with regard to absence of human DNA, DNase, RNase and PCR inhibitors. Consumables with the PCR clean purity grade are controlled and certified by an external laboratory. Certificates are available for downloading from our webpage www.eppendorf.com.

Procedure

Sequence of commands that are executed one after the other. Part of an application.

Rack

Mount for tubes or pipette tips.

Operating instructions epMotion® 5070 11

English (EN)

Random error

Precision. Standard deviation of the average value of the dispensed volumes.

Remaining volume

Volume that cannot be aspirated from a vessel. The distance from the pipette tip to the vessel bottom, defined in the software, and the defined immersion depth of the pipette tip in the liquid, must be observed. Therefore, the pipette tip cannot aspirate the volume. The remaining volume depends on the vessel geometry.

Reservoir

Reservoirs are used to hold reagents. Reservoirs are hung in a Reservoir Rack or placed directly on the worktable.

SafeRack

Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination of adjacent tips. Use the SafeRacks if you would like to use tips several times.

Source labware

Labware that liquid is aspirated from during the application. Source labware refers to a plate or rack.

Source position

Position that liquid is aspirated from during the application.

Source vessel

The vessel from which liquid is aspirated during the application.

Sterile

Sterile is an Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. Sterile also meets the requirements with regard to sterility and freedom from pyrogens.

Systematic error

Accuracy. Deviation of the average value of the dispensed volumes from the selected volume.

Thermoadapter

Heat-conductive adapter for holding plates.

Thermoblock

A thermoadapter that is permanently connected to a PCR plate or PCR tube.

Thermorack

Temperable rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.

Operating instructions

12epMotion® 5070 English (EN)

Vessel

Tube or single well of a plate.

Work volume

Liquid volume of a labware. The epMotion fills a vessel with low levels of contamination up to the working volume. The working volume is less than the filling volume.

Worktable

Work surface of the epMotion where labware and tools are placed. In the software, the epMotion worktable is shown as the epBlue worktable.

1.6Revision history

Revision history for the hardware operating manuals for the epMotion 5070, order number 5070 900.850.

Version of the operating manual	Date	Changes

Version 00	June 2013	Created new

Product description

epMotion® 5070	13
English (EN)

2Product description

2.1Main illustration

2.1.1epMotion 5070

1	2	3	y
			y

		epMotion	5070
		epMotion
9	8	7		6	5	4

Fig. 2-1: Main illustration epMotion 5070

1	Carrier	6	Worktable
	The carrier moves in X-direction, Y-direction and		Work surface for tools and labware.
	Z-direction.	7	EasyCon
		7	EasyCon
2	Tool holder		Control panel used to control the epMotion.
	Holds dispensing tools.	8	Front hood
		8	Front hood
3	Optical sensor		Safety device for protection from movable parts
	Detects levels, tips and labware.		and contamination.
4	Mains power connection	9	Mains power switch
5	Interfaces

Product description

14epMotion® 5070 English (EN)

2.1.2	epMotion 5070f
						z
	1	2	3		y
					y
						x
			epMotion	5070
			epMotion
9	8		7		6	5	4

Fig. 2-2: Main illustration epMotion 5070f

1	Carrier	6	Worktable
	The carrier moves in X-direction, Y-direction and		Work surface for tools and labware.
	Z-direction.	7	EasyCon
		7	EasyCon
2	Tool holder		Control panel used to control the epMotion.
	Holds dispensing tools.	8	Mains power switch
		8	Mains power switch
3	Optical sensor	9	Light barrier
	Detects levels, tips and labware.	9	Light barrier
	Detects levels, tips and labware.		The light reflectors are located on the inside of
			The light reflectors are located on the inside of
4	Mains power connection		the front screen of the Cleanbench.
5	Interfaces

Product description epMotion® 5070 15

English (EN)

2.1.3Interfaces

Ethernet

USB

Fig. 2-3: Interfaces epMotion 5070 and 5070f

Ethernet

USB

Connection for the cable to the EasyCon

Connection for a USB storage medium for

firmware updates.

Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected to the interfaces.

Product description

16epMotion® 5070 English (EN)

2.1.4 EasyCon

Fig. 2-4: EasyCon

The EasyCon is the control panel of the epMotion. The EasyCon has a touch-sensitive color display (touchscreen). A key pad will be shown on the display for data entry.

Fig. 2-5: InterfacesEasyCon
1 USB	2 Ethernet
Connections for mouse with a USB connection	Connection for the epMotion cable
cable or USB storage medium

Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected to the interfaces.

Product description	17
epMotion® 5070	17
English (EN)

2.2Delivery package

Quantit	Order no.	Description
y	(International)

		Automated pipetting system epMotion 5070
		main device, with EasyCon, accessories, installed software epBlue
1	5070 000.263	100 V - 240 V, 50 / 60 Hz

		Automated pipetting system epMotion 5070f
		main device for cleanbench, with EasyCon, accessories,
		installed software epBlue
or 1	5070 000.264	100 V - 240 V, 50 / 60 Hz

		EasyCon
1	5073 005.002	Control panel

		Mouse
1	-	With USB connection cable

		Cable
1	-	For connecting the EasyCon to the epMotion

		Mains power cable
1	-	Compatible with the country where the order was placed or defined

		USB cable
1	-	For connecting USB devices to the EasyCon

1	-	Tool for transport securing device
1	5075 753.006	Waste box
		Operating Manual
1	5070 900.850	epMotion 5070

		Operating Manual
1	5075 901.250	software operating manual epBlue with EasyCon

Product description

18epMotion® 5070 English (EN)

2.3Features

The epMotion 5070 is available with different features. All available features are described in this manual.

2.3.1Function

The epMotion 5070 is an automatic system for dispensing liquids using dispensing tools and ep.T.I.P.S. Motion pipette tips.

The epMotion 5070 dispenses liquids in the volume range 1 μL – 1 000 μL.

The dispensing tool aspirates liquid from source vessels and dispenses this liquid in destination vessels. The dispensing tool works according to the piston-stroke principle. For the epMotion, dispensing tools are available for 3 different volume ranges.

The epMotion features an optical sensor. The sensor checks:

•Type and location of labware

•Quantity and position of pipette tips in the rack

•Filling level of vessels

You control the epMotion 5070 via the EasyCon control unit with the epBlue software.

You can use the epBlue software to define dispensing processes and compile them into an application. To do so, you select source vessels and destination vessels, define the procedure and define the transfer pattern.

2.3.2Cleanbench

The epMotion 5070f must only be operated in a Cleanbench. The function corresponds to a epMotion 5070 without a Cleanbench.

Additional information on your epMotion is available at: www.eppendorf.com/automation.

Safety	19
epMotion® 5070	19
English (EN)

3Safety

3.1Intended use

3.1.1Intended use of the epMotion 5070

The device is intended for use in laboratories for research, development, and industrial and routine work, as well as for training purposes. The areas of applications include – but are not limited to – the fields of life sciences, biotechnology, chemistry and clinical research.

The automated pipetting system epMotion 5070 is designed for the monitoring of contamination-free, precise and correct dispensing and transferring of liquids and to automatically check the combining of liquids.

The autoclavable dispensing tools work in a volume range from 1 μL to 1000 μL.

The device meets the basic, essential requirements of the EC Directives and Standards that are listed in the declaration of conformity, but the product is not registered with the FDA.

These automated pipetting systems are exclusively intended for use indoors and may only be used by skilled personnel who have received adequate training.

3.1.2Intended use of the epMotion 5070f

The automated pipetting system epMotion 5070f is designed for the monitoring of contamination-free, precise and correct dispensing and transferring of liquids and to check the combining of liquids automatically.

The autoclavable dispensing tools work in a volume range from 1 μL to 1000 μL.

The epMotion 5070f must only be operated in a Cleanbench.

The device meets the basic, essential requirements of the EC Directives and Standards that are listed in the declaration of conformity, but the product is not registered with the FDA.

These automated pipetting systems are exclusively intended for use indoors and may only be used by skilled personnel who have received adequate training.

3.2User profile

This device may only be operated by skilled personnel.

The skilled personnel must have received training on this device. The training must have been conducted by Eppendorf AG or an authorized partner of Eppendorf AG.

The skilled personnel must have read the operating manual carefully. The skilled personnel must have read the operating manuals of all software components carefully.

Safety

20epMotion® 5070 English (EN)

3.3Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

•The device is not used in accordance with the operating manual.

•The device is used outside of its intended use.

•The device is used with accessories or consumables which are not recommended by Eppendorf.

•The device is maintained or repaired by people not authorized by Eppendorf.

•The user makes unauthorized changes to the device.

3.4Warnings for intended use

DANGER! Risk of explosion.

Do not operate the device in areas where work is completed with explosive substances.

Do not use this device to process any explosive or highly reactive substances.

Do not use this device to process any substances which may generate an explosive atmosphere.

WARNING! Lethal voltages inside the device.

Ensure that the housing is always closed and undamaged so that no parts inside the device can be contacted by accident.

Do not remove the housing of the device.

Do not allow any liquids to penetrate the inside of the housing.

Do not allow the device to be opened by anyone except service personnel who have been specifically authorized by Eppendorf.

WARNING! Electric shock due to damage to device or mains cable.

Only switch on the device if the device and mains cable are undamaged.

Only use devices that have been properly installed or repaired.

In case of danger, disconnect the device from the mains supply by pulling the power plug from the device or the mains socket or, by using the isolating device intended for this purpose (e.g. emergency stop switch in the laboratory).

WARNING! Risk from incorrect supply voltage

Only connect the device to voltage sources which correspond to the electrical requirements on the name plate.

Only use sockets with a protective earth (PE) conductor and suitable power cable.

Safety	21
epMotion® 5070	21
English (EN)

WARNING! Damage to health due to infectious liquids and pathogenic germs.

When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

Wear personal protective equipment.

For full instructions regarding the handling of germs or biological material of risk group II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual).

WARNING! Health hazard from skin contact with infectious substances.

Wear protective gloves.

WARNING! Danger due to flammable and infectious liquids in the waste container.

Pipette tips in the waste container may contain flammable or infectious liquids.

Wear personal protective equipment.

Handle the pipette tips and sample materials from the waste container in accordance with the material safety data sheets, safety regulations and laboratory guidelines.

WARNING! Damage to health due to contaminated device and accessories.

Decontaminate the device and the accessories before storage and shipping.

CAUTION! Risk of injury due to carrier movement.

The carrier may still be moving when the front hood of the epMotion is opened.

Wait until the carrier stops moving before reaching into the device.

CAUTION! Cuts due to broken glass.

A damaged touchscreen leads to cuts on the hands.

Only use the EasyCon if it is not damaged.

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.

Only use accessories and original spare parts recommended by Eppendorf.

NOTICE! Spilled liquid can cause damage to the device.

Switch off the device.

Disconnect the power plug.

Collect the spilled liquid. Observe the specifications for the liquid in the material safety data sheets.

NOTICE! Size of disposables can change through autoclaving.

Do not use autoclaved disposable products in automated applications.

Safety

22epMotion® 5070 English (EN)

3.5Hazard symbols and safety devices on the device

This section explains the warning symbols on the epMotion and the location of the safety devices.

epMotion 5070			epMotion 5070f
1	2		1	2
	epMotion	5070		epMotion	5070
	epMotion			epMotion

	4

Fig. 3-1:	Hazard symbols and safety devices on the epMotion 5070
Tab. 3-1: Hazard symbols

1	WARNING
	Observe the operating manual.

2	WARNING
	The carrier may continue moving after the front panel has
	been opened.

Wait until the carrier stops moving before reaching into the device.

Tab. 3-2: Safety devices

4		The housing or the cleanbench protects the user from
		contamination and moving parts.

Installation	23
epMotion® 5070	23
English (EN)

4Installation

4.1Selecting the location

Information on ambient conditions, dimensions and weights can be found in the technical data (see Weight/ dimensions on p. 85).

Select the location for the device according to the following criteria:

Electrical connections

•Mains connection in accordance with the name plate.

•The device mains/power switch and cutting unit of the power system circuit (e.g., FI protective switch) can be accessed during operation.

Location

•The ambient conditions match the specifications in the technical data.

•The location is well ventilated.

•The location is protected from direct sunlight.

•The location is not next to heat sources, such as heaters or drier compartments.

•There is an adequate amount of space available for the device. The minimum distances to other devices and walls is 6 cm.

•The device can be safely and easily operated at this location.

Workplace

•The lab bench is permanently mounted.

•The lab bench is designed for the weight of the device.

•The lab bench has a horizontal, plane work surface.

•The lab bench has a non-slip surface.

•The lab bench is vibration-free.

•There are no vibrating devices on the lab bench.

Cleanbench

•The Cleanbench is 60 cm deep.

•The Cleanbench cables are routed along its side.

•The laminar airflow in the Cleanbench is not affected by the device.

•There are light reflectors on the front screen of the Cleanbench.

4.2Installing the device

The epMotion may only be installed and commissioned by skilled personnel authorized by

Eppendorf.

Information on transport (see Transport on p. 83).

Installation

24epMotion® 5070 English (EN)

Hardware	25
epMotion® 5070	25
English (EN)

5Hardware

5.1Worktable for epMotion 5070 and 5070f










Fig. 5-1: Worktable for epMotion
A1-B2						Waste
Locations for labware						Location for waste container
T1-T2
Locations for dispensing tools

Hardware

26epMotion® 5070 English (EN)

5.2 Optical sensor

Detector

Diaphragm

Semi-transparent

mirror

Diaphragm Light source

Imaging optics

Fig. 5-2: Optical sensor principle

On the carrier, the optical sensor is located to the right of the tool holder.

The optical sensor records the intensity of the reflected light using a lateral infrared light source, semi-transparent mirror, fixed lens and the procedure in z-direction. Height z0 is defined as the point with the highest intensity which enables the detection of the level in a vessel or the presence of labware.

The optical sensor can detect horizontal, plane surfaces. The surface of the liquid must be at an angle of 90° ± 3° to the optical axis.

The surfaces of liquids can be strongly bent by the vessel geometry or the physical properties of the liquid or vessel. The optical sensor may not detect the level on strongly bent surfaces. If this is the case, the user must enter the liquid volume.

The optical sensor has a detection limit for vessel levels. The detection limit depends on the vessel geometry. Generally, levels of 3 mm or higher can be detected.

The following functions of the optical sensor can be activated:

•Liquid detection

Determines the filling level for positions of labware, which has the Liquid detection option activated.

•Tip detection

Checks to see if the pipette tips defined in the application are available. Determines the quantity and position of tips in the rack.

Works only with epT.I.P.S. Motion in TipHolders and Racks from Eppendorf.

•Location detection

Detects the labware coding.

Checks to see if the labware on the epMotion worktable matches the labware on the epBlue worktable.

Information on setting the optical sensor, and a detailed description of all functions, can be found in the software operating manual.

Hardware	27
epMotion® 5070	27
English (EN)

5.3Tools

NOTICE! Damage to the gold contacts from handling.

The tool is damaged if the gold contacts on the tool become damaged or contaminated.

Do not touch the gold contacts.

The tool holder picks up a dispensing tool and puts it down again. The tool holder recognizes the tool from its gold contacts.

5.3.1Dispensing Tools

Fig. 5-3: Single-channel dispensing tool and eight-channel dispensing tool

The dispensing tools are piston-stroke pipettes. The function of the dispensing tools is described in the chapter (see Dispensing liquids on p. 29).

Single-channel dispensing tools and eight-channel dispensing tools, with 3 volume ranges each, are available for the epMotion.

Tab. 5-1: Volume range of the dispensing tools

Single-channel dispensing tool	Multi-channel dispensing tool	Volume range

TS 50	TM 50	1 μL – 50 μL

TS 300	TM 300	20 μL – 300 μL

TS 10000	TM 1000	40 μL – 1000 μL

The dispensing tool errors can be found in the technical data (see p. 87).

Help on dispensing errors can be found in the "Maintenance" chapter (see Dispensing errors on p. 81)

Hardware

28epMotion® 5070 English (EN)

Dispensing liquids	29
epMotion® 5070	29
English (EN)

6Dispensing liquids

6.1Basic procedure for dispensing

6.1.1Operating principle of dispensing tools

upper end position

basic position end of blow-out

	A	B	C

Fig. 6-1: Piston-stroke pipette principle
A	Aspirate	C Blow-out
	The piston moves upward from the home position and	Dispenses the residual liquid
	aspirates liquid.	Blow-out in the Pipette dispensing mode
B	Dispense	Remaining stroke in the Multidispense
B	Dispense	dispensing mode
	Liquid dispensing.	dispensing mode
	Liquid dispensing.

Multidispense: Piston travels to the home position in separate steps.

Pipette: Piston travels to the home position in one step.

The dispensing tools are piston-stroke pipettes that function according to the air cushion principle.

If the piston moves upward in the dispensing tool, liquid will be aspirated into the pipette tip. If the piston moves downward in the dispensing tool, liquid will be dispensed from the pipette tip. The pistons will be moved by a motor in the tool holder.

On eight-channel dispensing tools, all 8 channels move simultaneously.

Dispensing liquids

30epMotion® 5070 English (EN)

6.1.2 Liquid aspiration up to the remaining volume

0,7 mm 1 mm

Fig. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth

The pipette tip is immersed 3 mm into the liquid before liquid aspiration. The pipette tip moves downward during liquid aspiration. The immersion depth of 3 mm remains constant while the liquid level decreases.

If the pipette tip reaches a distance of 1 mm to the base of the vessel, the pipette tip reduces its immersion depth to 0.7 mm. If the liquid level continues to sink, the pipette tip will not aspirate any additional liquid. The remaining volume remains in the vessel.

A remaining volume is present because the pipette tip has a minimum immersion depth in the liquid and a minimum distance to the base of the vessel. Under normal conditions, the remaining volume is calculated using a filling level of 1.7 mm.

The distance of the pipette tip to the base of the vessel can be changed in the software (refer to epMotion software operating manual).

If the liquid is aspirated from the remaining volume, the bend of the liquid level can lead to dispensing errors.

6.1.3Bottom tolerance

The bottom tolerance is the distance between the vessel base and the lower end of the pipette tip. The default setting for the bottom tolerance is 1 mm. For 30 mL and 100 mL reservoirs, the default setting for the bottom tolerance is 2.5 mm.

Decreasing the bottom tolerance also reduces the remaining volume.

1.Removing liquid above a pellet increases the bottom tolerance.

2.Do not decrease the bottom tolerance on uneven plates.

3.Check the set bottom tolerance when exchanging pipette tips, plates or vessels.

+ 70 hidden pages