Eppendorf Multipette Xstream User Manual

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Multipette®/Repeater ® Xstream Multipette®/Repeater ® stream

Operating manual

Copyright© 2014 Eppendorf AG, Hamburg. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner.

Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany.

Combitips®, Combitips advanced®, Combitips plus®, Biopur®, Multipette stream®, Multipette Xstream®, PhysioCare Concept®, Repeater® are registered trademarks of Eppendorf AG, Hamburg, Germany.

Repeater stream™ and Repeater Xstream™ are protected trademarks of Eppendorf AG, Hamburg, Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

4986 900.115-05/092014

Table of contents

Multipette®/Repeater ® (X)stream 3

English (EN)

Table of contents

1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1.4 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 2.3.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 2.3.2 Dispensing tasks and ranges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.4 Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.5 Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.6 Overview Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.6.1 Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 2.6.2 Volume ranges for Combitips advanced . . . . . . . . . . . . . . . . . . . . 14

2.7 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.1 Mains/power supply device assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.2 Inserting the Li-ion rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.3 Charge the rechargeable battery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 4.4 Assembling the Combitip advanced and Adapter advanced. . . . . . . . . . . . . 21 4.5 Inserting the Combitip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 4.6 Removing the Combitip. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.6.1Finishing the dispensing operation and removing the Combitip . 23

4.6.2 Emptying and removing the Combitip. . . . . . . . . . . . . . . . . . . . . . 23

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.1 Principle of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 5.2 Prepare the Multipette stream/Repeater stream. . . . . . . . . . . . . . . . . . . . . . 25 5.3 Parameters of the Multipette stream/Repeater stream . . . . . . . . . . . . . . . . . 26 5.4 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.5 Automatic dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 5.6 Sequential dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 5.7 Aspirating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Table of contents

4Multipette®/Repeater ® (X)stream English (EN)

5.8 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 5.9 Titration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 5.10 Stopping or canceling pipetting operations . . . . . . . . . . . . . . . . . . . . . . . . . 34 5.11 Adjusting the device settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

6.1 Error search. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 6.2 Pipette self-test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 6.3 Hardware reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

7 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

7.2 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

8.1 Errors with the Multipette (X)stream/Repeater (X)stream . . . . . . . . . . . . . . 42

9 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.1 Multipette stream/Repeater stream . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 9.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 9.3 Combitips advanced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 9.4 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

10.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 10.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 10.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

11 Patents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Operating instructions

Multipette®/Repeater ® (X)stream 5

English (EN)

1 Operating instructions

1.1Using this manual

Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable.

The instructions for use for the Combitips advanced can be found on our webpage www.eppendorf.com/cbt.

This operating manual is part of the product. Please keep it in a place that is easily accessible.

Enclose this operating manual when transferring the device to third parties.

If this manual is lost, please request another one. You will find the current version on our webpage www.eppendorf.com/worldwide.

If information in this manual refers to the Multipette stream/Repeater stream, it is also valid for the Multipette Xstream/Repeater Xstream. If information is only valid for the Multipette Xstream/Repeater Xstream, this is explicitly mentioned in the text.

This manual applies to devices with software version 03.06.00 and higher. For devices with older software and hardware, use the operating manuals Multipette (X)stream/ Repeater (X)stream 4986 900.115-01.

1.2Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1 Danger symbols

Biohazard	Explosion
Electric shock	Toxic substances
Hazard point	Material damage

1.2.2 Danger levels

DANGER	Will lead to severe injuries or death.
WARNING	Can lead to severe injuries or death.
CAUTION	Can lead to light to moderate injuries.
ATTENTION	May lead to material damage.

Operating instructions

6Multipette®/Repeater ® (X)stream English (EN)

1.3Symbols used

Depiction					Meaning
1.					Actions in the specified order
2.
					Actions without a specified order
•					List:
1					Step in the figures
					Direction of movement
Text					Display or software texts
					Additional information
1.4				Glossary
A

Adapter advanced

Connecting piece for the dispenser when using Combitips advanced 25 mL and 50 mL

B

Biopur

Eppendorf Biopur® is an Eppendorf AG purity grade for consumables. Eppendorf Biopur® meets the requirements for standard products, e.g. precision, accuracy, wetting behavior, tightness. Eppendorf Biopur® also meets the requirements with regard to sterility, absence of ATP, PCR inhibitors, human and bacterial DNA, pyrogen, DNase and RNase. Consumables of the purity grade Biopur are controlled and certified by an external laboratory.

Certificates are available for downloading from our internet page www.eppendorf.com.

C

Combitip advanced

Consumables for all Eppendorf Multipettes and Repeaters. With the Combitip advanced, the dispenser becomes an operational dispenser. Combitips advanced are intended for single use. Combitips advanced consist of a piston and a cylinder and function according to the positive displacement principle. They are offered for nine different volume ranges. For Combitips advanced with a maximum volume of 25 mL or 50 mL, the Adapter advanced is also required.

Operating instructions

Multipette®/Repeater ® (X)stream 7

English (EN)

F

Free jet dispensing

Dispensing the liquid without the Combitip touching the tube inner wall. If a drip forms on the Combitip after free jet dispensing, this drip always belongs to the next dispensing step. We recommend completing a dispensing series using only the free jet method or wall dispensing method. Complete the reverse stroke in accordance with the dispensing series using either the free jet or wall dispensing method. Errors listed in the technical data were determined using the wall dispensing method.

I

Increment

Step size or resolution. For instance, when selecting the volume for a 10 mL Combitip, the volume can be increased or reduced in 0.01 mL steps.

ISO 8655

The ISO 8655 standard defines limiting values for systematic errors [accuracy], random errors [precision]) and the test methods for dispensing devices.

N

Nominal volume

The maximum dispensing volume of a Combitip in conjunction with the selected dispensing device. The term "nominal volume" comes from the ISO 8655 standard.

P

PhysioCare Concept®

Products that carry the Eppendorf PhysioCare Concept logo feature optimized ergonomics and make operation as simple as possible, even with complex dispensing tasks. PhysioCare products can be easily serviced and cleaned. They are made of extremely light materials and require minimal operating force.

Positive displacement principle

The liquid comes into direct contact with the Combitip piston during aspiration and dispensing. Unlike with a pipette, the liquid and piston are not separated by an air cushion. A little air bubble is visible at the piston during dispensing.

R

Rechargeable lithium-ion battery

Rechargeable lithium-ion batteries have a very high charging capacity and long service life.

Reverse stroke

After aspiration, the piston is moved into a defined position. Liquid is dispensed during this piston movement. The reverse stroke is not a dispensing step.

Operating instructions

8Multipette®/Repeater ® (X)stream English (EN)

V

Viscosity

Viscosity describes the viscidity of liquids and suspensions. The dynamic or absolute viscosity is indicated in Pa·s or in mPa·s. In older literature, the unit P or cP is used (1 mPa·s corresponds to 1 cP).

At room temperature, a 50 % glycerin solution has a viscosity of approx. 6 mPa·s. As the glycerin concentration increases, viscosity increases considerably. Absolutely anhydrous glycerin has a viscosity of approx. 1480 mPa · s at room temperature.

Product description

Multipette®/Repeater ® (X)stream 9

English (EN)

2 Product description

2.1Main illustration

			1	1	Connector socket
					For connecting the charging plug of an Eppendorf
	Asp		2
					Multipette stream/Repeater stream power supply
		Pip
	Seq	Ttr
	Ads Dis		3		device.
			4	2	Hardware reset button
			5		Triggering a hardware reset interrupts the power
select	reset	+ / –			supply (see p. 40).
			6	3	Selection dial
			7
			8		For setting the operating mode.
			9		Depending on the Multipette stream/Repeater
					stream version, different operating modes are
					available. The illustration shows the Xstream.
				4	Display (illuminated)
					Displays the parameter settings (volume, dispensing
					steps, speeds) and provides feedback and
					information on the operating steps.
				5	Select rocker switch
					For switching between the execution and editing
					displays.
				6	Reset key
					For stopping or canceling dispensing operations
					(see p. 34).
				7	+/- rocker switch
					For changing parameters. Higher speed level with
					volume change when you press the +/- rocker switch
					more firmly.
			10	8	Actuate key
					For triggering dispensing operations. For saving
					entries during editing and for returning to
					dispensing.
				9	Ejector
					Releases the inserted Combitip.
				10	Combitip advanced
					Dispenser tip
Fig. 2-1:		Front view

Product description

10Multipette®/Repeater ® (X)stream English (EN)

1

2

3 4

Xstream

®

Multipette

1Viewing window for the infrared interface

For Service.

2Battery compartment lid

For covering the battery compartment. The battery compartment contains the serial number of the Multipette stream/Repeater stream.

3Charging contact and mounting

For the battery charging option and for fixing the Multipette stream/Repeater stream in the charging stand (additionally available).

4Finger rest

For ensuring a secure and comfortable grip.

Fig. 2-2:

Side view

2.2Delivery package

Quantity	Description
1	Multipette stream
or	Multipette Xstream
or	Repeater stream
or	Repeater Xstream
1	Combitips advanced 1.0 mL
1	Adapter advanced 25 mL
1	Adapter advanced 50 mL
1	Li-ion rechargeable battery
1	Power supply with power plug adapters
1	Operating Manual Multipette/Repeater (X)stream
1	Short instructions Multipette/Repeater (X)stream

Product description

Multipette®/Repeater ® (X)stream 11

English (EN)

2.3Features

In combination with a Combitip, the Multipette stream/Repeater stream is a motor-driven and electronically controlled manual dispenser which operates according to the positive displacement principle.

2.3.1 Operating modes

The Multipette stream/Repeater stream and Multipette Xstream/Repeater Xstream have the following operating modes:

			Dis		Ads	Seq		Asp	Pip	Ttr
Multipette stream/Repeater stream			●		●	–		–	●	–
Multipette Xstream/Repeater Xstream			●		●	●		●	●	●
Dis	Dispensing	gradual dispensing of liquid in equal partial volumes.
Ads	Automatic	automatic dispensing of liquid in equal partial volumes
	dispensing	and at fixed intervals.
Seq	Sequential	dispensing liquid in up to 16 different volumes in one
	dispensing	sequence.
Asp	Aspirating	consecutive aspiration of liquid of the same volume
		into a Combitip.
Pip	Pipetting	aspirating and dispensing liquid.
Ttr	Titration	gradual dispensing of reagent into a sample solution.
		Multiple aspiration of the reagent into the Combitip is
		possible.

2.3.2 Dispensing tasks and ranges

It is possible to dispense aqueous solutions, but also solutions with a higher viscosity. At a higher viscosity, lower aspiration and dispensing speeds should be selected.

•If the lowest speed (1 bar) is used for aspiration and dispensing, it is possible to dispense solutions with a viscosity of ≤ 300 mPa·s with ≤ 25 mL Combitips.

•If 50 mL Combitips are used, the viscosity must not exceed 100 mPa·s.

•With 0.5 mL Combitips and the highest speed setting, 5 μL of water can be dispensed in a free jet. Free jet dispensing of water is also possible with all larger Combitips. If large volumes are dispensed in a free jet, the liquid may splash back!

For solutions with a higher vapor pressure it is recommended to saturate the small air bubble at the piston of the Combitip with liquid vapor by aspirating and dispensing the liquid several times.

Product description

12Multipette®/Repeater ® (X)stream English (EN)

2.4Warranty

In the case of warranty claims, please contact your responsible Eppendorf contractual partner. No warranty is provided for any damage due to misuse or if the device has been opened by unauthorized persons. The rechargeable lithium-ion battery and all other wear parts are excluded from the warranty.

2.5Display

The display has 10 brightness levels. The display switches off during usage intervals. The display switches on again automatically when a key is pressed. If the Multipette stream/ Repeater stream is not used for an extended period of time, the display is switched off completely after approx. 10 minutes.

The Multipette stream/Repeater stream saves the most recent setting for each Combitip size in all operating modes. If you are using a new Combitip in the corresponding size, the most recent setting will be shown on the display.

Product description

Multipette®/Repeater ® (X)stream 13 English (EN)

2	3	4
100.0µL •		20
1	6	5
Fig. 2-3:	Display

1Volume

Unit in μL or mL.

2Direction of the next piston movement

Aspirate liquid

Dispense liquid

3Key lock

is only displayed if the +/- rocker switch is locked. Can be stored or activated for the device parameters (see p. 34).

4Battery

Battery charging status. Possible displays: Battery full

Battery exhausted

Battery severely discharged

Battery empty

If the battery is exhausted, complete the dispensing operation and charge the battery. If the battery is severely discharged, or Warning Battery Low! appears on the display, stop the dispensing operation immediately and charge the battery for at least two hours.

5Dispensing step

In the Dis, Ads and Seq modes, the number of possible dispensing steps. In the Asp mode, the number of performed Asp steps

6Interpunct

Is displayed in the Dis, Ads and Asp modes before aspiration. Indicator for the number of dispensing steps. The pipetted volume must always be greater than the product of the multiplication of the volume (1) with the dispensing step (5).

2.6Overview Combitips advanced

Combitips advanced are disposable devices for aspirating and dispensing liquids according to the positive displacement principle. Combitips advanced are used in Eppendorf dispensers (e.g., Multipettes/Repeaters) and are made up of a cylinder and a piston. Combitips advanced in sizes 25 mL and 50 mL require an Adapter advanced. Combitips advanced are available in various sizes, which are marked using a color code.

Product description

14Multipette®/Repeater ® (X)stream English (EN)

2.6.1 Combitips advanced

^
0.1 mL 0.2 mL 0.5 mL	1 mL	2.5 mL	5 mL	10 mL	25 mL	50 mL

2.6.2 Volume ranges for Combitips advanced

Combitips advanced	Volume range	Increment
0.1 mL	1.0 μL – 100 μL	0.1 μL
white
0.2 mL	2.0 μL – 200 μL	0.2 μL
light blue
0.5 mL	5.0 μL – 500 μL	0.5 μL
purple
1.0 mL	10 μL – 1000 μL	1.0 μL
yellow
2.5 mL	25 μL – 2500 μL	2.5 μL
green
5.0 mL	50 μL – 5000 μL	5.0 μL
blue
10 mL	0.1 mL – 10 mL	0.01 mL
orange
25 mL	0.25 mL – 25 mL	0.025 mL
red
50 mL	0.5 mL – 50 mL	0.05 mL
light gray

Product description

Multipette®/Repeater ® (X)stream 15

English (EN)

2.7Materials

NOTICE! Aggressive substances may damage dispensers, Combitips and accessories.

Check the chemical resistance when using organic solvents or aggressive chemicals.

Please note the cleaning instructions.

The components of the Multipette stream/Repeater stream which can be accessed by the user are made from the following materials:

Component	Material
Housing parts	Acrylonitrile/styrene/acrylic ester (ASA)
	with polycarbonate (PC)
Select rocker switch, +/- rocker switch	Silicone
Actuate key	Polyetherimide (PEI)
Charging contacts	Gold coating
Display, selection dial, window IR interface	Polycarbonate (PC), coated and uncoated
Combitip advanced	Material
Cylinder	Polypropylene (PP)
Piston 0.1 mL and 0.2 mL	Polyethylene (PE) with glass fiber (GF)
Piston 0.5 mL to 50 mL	Polyethylene (PE)
Adapter advanced	Polybutylene terephthalate (PBT)

Safety

16Multipette®/Repeater ® (X)stream English (EN)

3 Safety

3.1Intended use

The Multipette stream/Repeater stream is a lab device and in combination with a Combitip advanced it is intended for dispensing aqueous solutions in the volume range of 1 μL – 50 mL. In vivo applications (applications in or on the human body) are not permitted.

The !!! use DEV_MODEL !!!! may only be operated by trained specialists. All users must have read the operating manual carefully and familiarized themselves with the device's mode of operation.

The Multipette stream/Repeater stream is not suitable for the permanent dispensing of fuming acids.

3.2Warnings for intended use

DANGER! Risk of explosion.

Do not operate the device in areas where work is completed with explosive substances.

Do not use this device to process any explosive or highly reactive substances.

Do not use this device to process any materials that could create an explosive atmosphere.

WARNING! Damage to health from infectious liquids and pathogenic germs.

When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

Wear personal protective equipment.

Follow the instructions regarding hygiene, cleaning and decontamination.

Comprehensive regulations on handling germs or biological material in risk group II or higher can be found in the "Laboratory Biosafety Manual" (source: World Health Organization, Laboratory Biosafety Manual, in the currently valid version).

WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.

Wear personal protective equipment.

Observe the national regulations for handling these substances.

Observe the material safety data sheets and manufacturer's application notes.

Safety

Multipette®/Repeater ® (X)stream 17

English (EN)

CAUTION! Danger to persons from careless use

Never point the opening of a Multipette stream/Repeater stream which is equipped with a Combitip at yourself or other persons.

Only initiate dispensing if it is safe to do so.

With any dispensing task please ensure that you do not endanger yourself and other persons.

Check the set speed before commencing dispensing operations. For the dispensing operation select a speed which excludes the back-splashing of the liquid.

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.

Only use accessories and original spare parts recommended by Eppendorf.

NOTICE! Carry-over, contamination and incorrect dispensing results due to the incorrect use of Combitips.

Combitips are intended for single use. Prolonged use can have a negative impact on dispensing accuracy.

Only use Combitips once.

Do not use washed and/or autoclaved Combitips for dispensing.

NOTICE! Damage to the device due to penetration of liquids.

Do not allow any liquids to penetrate the inside of the housing.

If liquid has entered the inside of the housing, the inner parts may only be repaired by Eppendorf AG service partners. Contact your responsible sales office before returning a product.

NOTICE! Terminating or interrupting dispensing due to incorrect handling.

If the Combitip is handled incorrectly, an error message may appear on the display or a safety shutdown may be triggered.

Do not bend the Combitip.

During dispensing or aspirating, do not press the Combitip too strongly against the bottom of the tube or against the tube wall.

Safety

18Multipette®/Repeater ® (X)stream English (EN)

NOTICE! Material damage due to incorrect dispensing.

When using the larger Combitips and highly viscous solutions the Multipette may heat up.

Only use highly viscous solutions for short dispensing series.

NOTICE! Incorrect dispensing results due to evaporation.

If dispensing is continued with a Combitip that has already been filled after a waiting time, the dispensing volume in the next dispensing step may be slightly reduced due to evaporation.

If a high degree of accuracy is required, omit this dispensing step.

3.3Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

•The device is not used in accordance with the operating manual.

•The device is used outside of its intended use.

•The device is used with accessories or consumables which are not recommended by Eppendorf.

•The device is maintained or repaired by people not authorized by Eppendorf.

•The user makes unauthorized changes to the device.