Eppendorf FemtoJet 4x User Manual v.2

nual

EN) manual

www.eppendorf.com/myeppendorf

FemtoJet

Operating manual

Eppendorf

and the Eppendorf logo are registered trademarks of Eppendorf AG,

Hamburg, Germany.

FemtoJet

, Femtotips®, TransferMan® and InjectMan® are registered trademarks of

Eppendorf AG, Hamburg, Germany.

Registered trademarks and protected trademarks are not marked in all cases with

or ™

in this manual.

5253 900.018-00/012014

FemtoJet

English (EN)

Table of contents

1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.1 Danger symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1.1 Delivery package – Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Exemplary set-up of a microinjection system. . . . . . . . . . . . . . . . . . . . . . . . . 9

2.4 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.4.1 Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.5 Name plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.6 Control panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.7 Rotary knobs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2.8 Foot control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.9 Hand control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.10 Pressure parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.10.1 Compensation pressure p

2.10.2 Injection pressure p

2.10.3 Injection time t

2.10.4 Rinsing pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.10.5 Operating pressure p

2.11 Self-calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.12 Universal capillary holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.3 Warning signs on the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.5 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.1.1 Complaints about damages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.1.2 Incomplete delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Table of contents

FemtoJet

English (EN)

4.3 Connecting the external pressure supply . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.3.1 Preparing the external pressure supply . . . . . . . . . . . . . . . . . . . . 21

4.3.2 Connecting the compressor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.3.3 Connecting the compressed gas cylinder . . . . . . . . . . . . . . . . . . . 22

4.3.4 Connecting the compressed air supply by the domestic services. 22

4.4 Connecting the Microinjector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.5 Connecting an external device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.5.1 Connecting a micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.5.2 Connecting the PC. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.5.3 Connecting two devices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

4.6 Connecting accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

4.6.1 Connecting a foot control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

4.6.2 Connecting a hand control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

5.1 main screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

5.2 Main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.3 Navigating the menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.3.1 Selecting the menu and navigating . . . . . . . . . . . . . . . . . . . . . . . . 26

5.3.2 Exiting the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.3.3 Selecting parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.3.4 Changing a parameter value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.1 Switching on or off the Microinjector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.1.1 Switching on the Microinjector . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.1.2 Switching off the Microinjector. . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.2 Switching on or off the standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.2.1 Switching on standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

6.2.2 Switching off the standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.3 Determining the injection parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.3.1 Filling the capillaries with fluorescence dye . . . . . . . . . . . . . . . . . 29

6.3.2 Carrying out a test injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

6.3.3 Possible sources of error - cell inflates or bursts . . . . . . . . . . . . . 30

6.3.4 Possible sources of error - capillary is clogged. . . . . . . . . . . . . . . 30

6.3.5 Possible sources of error - liquid is not being injected. . . . . . . . . 30

6.3.6 Possible sources of error - capillary does not reach the cell. . . . . 30

6.3.7 Result – the injection parameters have been determined . . . . . . . 30

6.4 Setting the compensation pressure p

6.5 Setting the injection pressure p

6.6 Setting the injection time t

6.7 Setting the injection mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

6.7.1 Setting the automatic injection mode . . . . . . . . . . . . . . . . . . . . . . 32

6.7.2 Setting the manual injection mode . . . . . . . . . . . . . . . . . . . . . . . . 32

Table of contents

FemtoJet

English (EN)

6.8 Injecting liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

6.8.1 Automatically injecting liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

6.8.2 Manually injecting liquid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6.9 Replacing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6.10 Rinsing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6.11 Setting the injection counter to zero. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6.12 Calling up saved injection parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

6.12.1 Calling up saved injection parameters . . . . . . . . . . . . . . . . . . . . . 34

6.13 Saving or changing the injection parameters. . . . . . . . . . . . . . . . . . . . . . . . 34

6.13.1 Saving injection parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

6.13.2 Changing the saved injection parameters . . . . . . . . . . . . . . . . . . . 35

6.14 Adjusting the device settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

6.14.1 Change capillary function – Changing the capillary . . . . . . . . . . . 35

6.14.2 Pressure unit function – Selecting the pressure unit . . . . . . . . . . 35

6.14.3 Beeper function – Switching on/off the signal tone . . . . . . . . . . . 35

6.14.4 Contrast function – Setting the display contrast . . . . . . . . . . . . . . 35

6.14.5 Illumination function – Switching on/off the display illumination 36

6.14.6 Continuous flow function – Setting the constant operating pressure 36

6.15 Inserting the capillary into the capillary holder . . . . . . . . . . . . . . . . . . . . . . 37

6.16 Inserting Femtotips into the capillary holder . . . . . . . . . . . . . . . . . . . . . . . . 37

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

7.2.1 Error 01 – 10 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

7.2.2 Error 11 – 18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

7.2.3 Error 19 – 38 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

7.2.4 Error 39 – 40 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

8.1 Replacing the sealings in the grip head . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

8.1.1 Removing the sealing washer and o-rings . . . . . . . . . . . . . . . . . . 43

8.1.2 Inserting the o-rings and the sealing washer . . . . . . . . . . . . . . . . 44

8.2 Replacing the sealing in the adapter for Femtotips . . . . . . . . . . . . . . . . . . . 44

8.2.1 Replacing the o-ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

8.3 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

8.4 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

8.5 Hints with regard to service intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

8.6 Service and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

8.7 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Table of contents

FemtoJet

English (EN)

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.1 Mode of operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.2 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.3 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.4 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.4.1 USB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.4.2 RS 232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.5 External compressed air supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.6 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

10.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

10.3 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

10.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

11 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

11.1 FemtoJet 4x . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

11.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

11.3 Universal capillary holder and accessories. . . . . . . . . . . . . . . . . . . . . . . . . . 52

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Operating instructions

FemtoJet

English (EN)

1 Operating instructions

1.1 Using this manual

 Read this operating manual completely before using the device for the first time. Also

observe the instructions for use of the accessories.

 This operating manual is part of the product. Thus, it must always be easily accessible.  Enclose this operating manual when transferring the device to third parties.  You will find the current version of the operating manual for all available languages on

our webpage under

1.2 Danger symbols and danger levels

The safety instructions of this operating manual indicate the following danger symbols and danger levels:

1.2.1 Danger symbols

Electric shock Hazard point

Cuts Material damage

Biohazard

www.eppendorf.com.

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Depiction Meaning

Actions in the specified order

 Actions without a specified order

• List

Text Display text or software text

Direction of movement

Additional information

Product description

FemtoJet

English (EN)

2 Product description

2.1 Delivery package

Number Description

1 FemtoJet 4x

1 Mains/power cord

1 Injection tube

1 Pressure tube with quick coupling and plug

1 Quick coupling (nickel plated), cylindrical thread

1 Quick coupling (brass), conical thread

1 Operating manual

2.1.1 Delivery package – Accessories

Number Description

1 Universal capillary holder

1 Grip head 0 for capillary (outer diameter 1.0 mm to 1.1 mm)

1 Adapter for Femtotips

1 Foot control

2.2 Features

The Microinjector FemtoJet 4x can be used to inject amounts of fluid of up to 1 µL into cells. The parameters for pressure and time are set on the device and controlled by the software. It is possible to trigger the injection on the Microinjector or on a connected micromanipulator by Eppendorf. The semiautomatic injection movement is controlled by the Microinjector or the micromanipulator. The required pressure is provided by an external pressure source (e.g. compressed air supply of domestic services or compressed gas cylinder).

2.3 Exemplary set-up of a microinjection system

Abb. 2-1:Microinjection system with FemtoJet 4x

Product description

FemtoJet

English (EN)

Fig. 2-1: Microinjection system with FemtoJet 4x

1 Micromanipulator InjectMan 4

2 Universal capillary holder with

capillary

3 Inverse microscope

4 Injection tube

5 Control board InjectMan 4

6 External pressure supply

Compressor, compressed gas cylinder or compressed air supply of domestic services

7 Pressure tubing

8 Microinjector FemtoJet 4x

FemtoJet

prog 1

prog 2

menu enter

auto man

inject

clean

count

Product description

FemtoJet

English (EN)

2.4 Main illustration

Abb. 2-2:Front and rear side

prog 1

menu enter

auto man

Fig. 2-2: Front and rear side

FemtoJet

prog 2

inject

clean

count

1 Display

2 Control panel

3 Port for injection tube

4 Rotary knobs for injection parameters

5 Name plate

Lower side of device

6 Interfaces

2.4.1 Interfaces

Abb. 2-3:Interfaces

RECTUS TYPE 21

pressure 4 - 8 bar

60 - 120 PSI

Fig. 2-3: Interfaces

324

MANIPULATOR

FOOT SWITCH

SERVICE

Product description

FemtoJet

English (EN)

1 Port for external pressure supply

2 Mains/power connection

3 Mains/power switch On/Off

4 Micro fuse

5 Port for micromanipulator or

computer

6 Connection for foot or hand control

7 Service connection

Product description

FemtoJet English (EN)

2.5 Name plate

Abb. 2-4:Name plate

3 4

Fig. 2-4: Name plate

1 Manufacturer

2 Product number

3 Voltage

4 Micro fuse

5 Frequency

9 10

6 Labelings

7 Address of manufacturer

8 Serial number

9 Output

10 Current consumption

Product description

FemtoJet

English (EN)

2.6 Control panel

The keys of the control panel are used to switch on the Microinjector, to perform functions, to select programs and to navigate through the menu.

Abb. 2-5:Control panel

5 6

prog 2prog 1prog 1

inject

menu enter

Fig. 2-5: Control panel

1 standby key

Activate/deactivate standby mode

2 menu enter key

Opens the menu

3 Arrow key

Navigating the menu

4 auto man key

Toggle between automatic and manual injection

5 prog 1 key

Select or save parameter set 1

auto man

cleanclean

countcountcountcount

6 prog 2 key

Select or save parameter set 2

7 inject key

Perform an injection

8 clean key

Clean the capillary

9 count key

Set the counter for performed injections to zero

Product description

enter

FemtoJet

English (EN)

2.7 Rotary knobs

The rotary knobs are used to set the injection parameters as injection time, injection pressure and compensation pressure.

Abb. 2-6:Rotary knobs

cleanclean

auto man

Fig. 2-6: Rotary knobs

1 Rotary knob

Set the injection pressure p

2 Rotary knob

Set the injection time t

countcountcountcount

321

3 Rotary knob

Set the compensation pressure p

2.8 Foot control

You can connect the foot control to the Microinjector.

The foot control corresponds to the following key on the Microinjector:

• Foot control – inject key

Product description

FemtoJet

English (EN)

Abb. 2-7:Foot control with plug

Fig. 2-7: Foot control with plug

2.9 Hand control

The hand control is not included in the delivery package and must be ordered separately.

You can connect the hand control to the Microinjector.

The hand controls correspond to the following keys on the Microinjector:

• Left hand control – inject key

• Right hand control – clean key

The inject and clean keys remain active when the hand control is connected.

2.10 Pressure parameters

The parameters are used to define the pressure and time for the injection and the cleaning of the capillary.

The pressure parameters include the following parameters:

The inject key remains active when the foot control is connected.

• Compensation pressure – p

• Injection pressure – p

• Injection time – t

• Operating pressure

• Rinsing pressure

Product description

FemtoJet

English (EN)

2.10.1 Compensation pressure p

The compensation pressure prevents the liquid from rising from the Petri dish into the capillary due to the capillary action. Due to the compensation pressure, some liquid will leak constantly from the capillary tip. This prevents the injection material from clumping together.

Abb. 2-8:Pressure ratios in the capillary

Fig. 2-8: Pressure ratios in the capillary

1 Capillary

2 Liquid with injection material

3 Compensation pressure p

4 Hydrostatic pressure

5 Capillary action

2.10.2 Injection pressure p

The injection pressure defines the pressure used for injecting liquid into the cell. During the injection process, the injection pressure is applied as long as the injection time is running. To inject liquid into a cell, the injection pressure must be higher than the inside pressure of the cell.

2.10.3 Injection time t

The injection time defines the time period for injecting the liquid. The start of the injection time depends on the presettings of the micromanipulator. The injection time begins either when triggering the injection function or when reaching the lower safety limit. The injection pressure is applied as long as the injection time is running.

2.10.4 Rinsing pressure

The rinsing pressure is used to clean the capillary.

Product description

FemtoJet

English (EN)

2.10.5 Operating pressure p

The operating pressure subsumes the injection pressure, compensation pressure and rinsing pressure.

2.11 Self-calibration

The Microinjector performs a calibration every two hours. During the calibration, the ventilation valve opens and the collected condensation water is discharged.

2.12 Universal capillary holder

You can insert a capillary or a Femtotip into the universal capillary holder.

Abb. 2-9:Universal capillary holder

1 2 3 4

6 7

Fig. 2-9: Universal capillary holder

1 Capillary

2 Grip head

3 Knurled screw

4 Universal capillary holder

5 Connection for injection tube

6 Femtotips

7 Adapter for Femtotips

Safety

FemtoJet English (EN)

3 Safety

3.1 Intended use

The FemtoJet 4x Microinjector is designed and manufactured for the exclusive use within the context of biologial, chemical and physical research.

Together with the micromanipulator and the capillary, the Microinjector forms a microinjection system. The Microinjector is used for the precise and reproducible injection of extremely small amounts of fluid (femto liter to micro liter range) in biological cells or nuclei.

The Microinjector is intended exclusively for indoor use and for operation by qualified staff.

3.2 Warnings for intended use

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries may detach themselves from the grip heads and become projectiles. Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.  Never aim capillaries at people.  Use capillaries with an outer diameter that matches the grip head

specifications.

 Always mount / dismount capillaries when they are depressurized.  Mount the capillary correctly in the grip head.  Do not touch the capillary with the Petri dish or other objects.

CAUTION! Risk of cuts from broken capillaries.

Capillaries are made of glass and are very fragile.

 Wear your personal protective equipment (PPE).  Always mount capillaries depressurized.  Never aim capillaries at people.  Handle the capillaries very carefully.

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