Eppendorf 5075 User Manual

Name: Eppendorf 5075
Brand: Eppendorf
SKU: 1469368
Rating: 5 (3 reviews)

5 (3)

075neN)manual

www.eppendorf.com/myeppendorf

epMotion® 5075

Hardware manual

Trademarks

Eppendorf®, the Epppendorf logo, epMotion®, Eppendorf Tubes ®, and epT.I.P.S.® are registered trademarks of Eppendorf AG.

LightCycler® and MagNA Pure® are registered trademarks of Roche Diagnostics.

epMotion VAC sealing foil U.S. Pat. No. 6,666,978

epMotion optical sensor U.S. Pat. No. 6,819,437

epMotion gripping tool U.S. Pat. No. 7,462,327

Registered trademarks are not marked in all cases with ® or ™ in this manual.

The software of the device (firmware) contains open source software. License information is available on request from Eppendorf AG.

Only for epMotion M5073, M5073c, 5075m and 5075mc: Limited Use Label License NOTICE TO PURCHASER; LIMITED LICENSE FOR RESEARCH USE ONLY.

This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The purchase price for this product includes only limited, nontransferable rights under certain claims of certain patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights are conveyed. Purchaser is not granted any rights under patents of Gen-Probe Incorporated to use this product for any commercial use. Further information regarding purchasing a license under patents of Gen-Probe Incorporated to use this product for any other purposes, including, without limitation, for commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development Department, 10210 Genetic Center Drive, San Diego, California 92121-4362, U.S.A.

5075 900.840-00/062013

Table of contents epMotion® 5075 3

English (EN)

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 1.6 Revision history. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 2.1.1 epMotion 5075 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 2.1.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 2.1.3 MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 2.2.1 epMotion 5075l and epMotion 5075lg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 2.2.2 epMotion 5075t and epMotion 5075tg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 2.2.3 epMotion 5075v and epMotion 5075vg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 2.2.4 epMotion 5075vt and epMotion 5075vtg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 2.2.5 epMotion 5075m and epMotion 5075mg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 2.2.6 Accessories for all versions of the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 2.2.7 Accessories for epMotion with epBlue ID. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 2.2.8 Accessories for epMotion with epBlue GxP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 2.3.1 Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 2.3.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 2.3.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 2.3.4 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 2.3.5 Magnetic separation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 2.3.6 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 2.3.7 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 3.1.1 Intended use of the epMotion 5075 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 3.5 Hazard symbols and safety devices on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 4.2 Installing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Table of contents

4epMotion® 5075 English (EN)

5 Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.1 Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 5.1.1 Layout of the epMotion 5075I worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 5.1.2 Layout of the epMotion 5075t worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 5.1.3 Layout of the epMotion 5075v worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 5.1.4 Layout of the epMotion 5075vt worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 5.1.5 Layout of the epMotion 5075m worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.2 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 5.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 5.3.1 Safety notes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 5.3.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 5.3.3 Equipping the thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.4 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 5.4.1 Safety notes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 5.4.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 5.4.3 Equipping the thermomixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.5 Magnet finger module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 5.5.1 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 5.5.2 Equipping the magnet finger module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.6 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 5.6.1 Vac Frame vacuum frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 5.6.2 VAC Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 5.6.3 Silicone mat for VAC Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 5.6.4 Vac Thermo Lid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 5.6.5 Elution plate adapter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 5.6.6 Loading the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.7 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 5.7.1 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 5.7.2 UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

5.8 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 5.9 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 5.9.1 Dispensing Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 5.9.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

6 Dispensing liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.1 Basic procedure for dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 6.1.1 Operating principle of dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 6.1.2 Liquid aspiration up to the remaining volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 6.1.3 Bottom tolerance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 6.1.4 Transporting and dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.2 Dispensing modes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 6.2.1 Dispensing mode: Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 6.2.2 Dispensing mode: Multidispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 6.2.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6.3 Special features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 6.3.1 Liquid aspiration from bottom of vessel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 6.3.2 Liquid aspiration from high vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 6.3.3 Liquid dispensing at the height of the vessel edge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 6.3.4 Liquid aspiration from a filter plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Table of contents epMotion® 5075 5

English (EN)

7 Labware – vessels, plates and pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.1 Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 7.2 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 7.2.1 Deepwell plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 7.2.2 Microplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 7.2.3 Filter plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 7.2.4 Channeling plate 96 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 7.2.5 PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 7.2.6 Tube plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

7.3 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 7.4 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 7.4.1 epT.I.P.S. Motion Racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 7.4.2 epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 7.4.3 Tip holder for epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 7.4.4 epT.I.P.S. Motion SafeRacks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

8 Labware accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

8.1 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 8.1.1 Racks for 24 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 8.1.2 Rack for 96 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72 8.1.3 Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 8.1.4 Thermorack and Rack 0.5/1.5/2.0 mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 8.1.5 PrepRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 8.1.6 ReagentRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 8.1.7 Reservoir Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76 8.1.8 ReservoirRack 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 8.1.9 Reservoir Rack modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

8.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 8.2.1 Height adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 8.2.2 Vac holder vacuum holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 8.2.3 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 8.2.4 Thermoadapter LC samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 8.2.5 Thermoadapter Frosty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.3 Thermoblocks and Thermoadapters for PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 8.3.1 Equipping the thermoblock with 0.2 mL vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 8.4 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 8.4.1 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 8.4.2 Waste funnel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 8.4.3 LiquidWasteTub with lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Table of contents

6epMotion® 5075 English (EN)

9 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

9.1 First steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 9.1.1 Switching on the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 9.1.2 Using the MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 9.1.3 Using the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

9.2 Loading the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 9.2.1 Display the loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 9.2.2 Positioning tools on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 9.2.3 Positioning the epT.I.P.S. Motion on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 9.2.4 Positioning the waste container on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 9.2.5 Positioning labware on the worktable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

9.3 Executing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 9.3.1 Performing a test run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 9.3.2 Starting the application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94 9.3.3 Controlling the application run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

9.4 Completing your work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 9.4.1 Cleaning up the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 9.4.2 Switching off the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

10.1 Decontamination and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 10.1.1 Carrying out wipe decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99 10.1.2 Carrying out spray decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 10.1.3 Carrying out UV irradiation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100 10.1.4 Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 10.3 Servicing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 10.4 Servicing the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 10.4.1 Replacing the seals of the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 10.4.2 Replacing the silicone mat of the vacuum lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 10.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

10.6 Carrying out firmware updates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102 10.7 Checking the dispensing volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102 10.8 Maintaining the dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103 10.8.1 Exchanging the sealing rings on the eight-channel dispensing tool . . . . . . . . . . . . . 103 10.9 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

11.1 Error search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105 11.2 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 11.2.1 Optical sensor errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 11.2.2 Dispensing errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107 11.2.3 Software errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

12.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 12.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 12.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110

Table of contents epMotion® 5075 7

English (EN)

13 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

13.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 13.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 13.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 13.4 Noise level. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 13.5 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112 13.6 MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112 13.7 Additional specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

13.7.1 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112 13.7.2 Carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112 13.7.3 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112 13.8 Enhancements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 13.8.1 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 13.8.2 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 13.8.3 Magnet finger module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 13.8.4 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 13.8.5 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 13.8.6 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 13.8.7 UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

13.9 Dispensing tool errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 13.9.1 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115 13.9.2 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116 13.10 Test conditions for dispensing tool errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

14.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 14.2 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 14.3 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119 14.4 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119 14.5 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 14.6 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 14.7 Thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 14.8 Accessories for the vacuum chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 14.9 MagSep kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 14.10 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122 14.11 Modification kits and enhancements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

Table of contents

8epMotion® 5075 English (EN)

Operating instructions epMotion® 5075 9

English (EN)

1Operating instructions

1.1Using this manual

Your epMotion operating manual consists of hardware instructions and software instructions. Short instructions are available for optional software enhancements.

The operating manual is part of the product.

The current version of the operating manual can be found on our webpage: www.eppendorf.com.

Read the operating manual in full before using the device.

Store the operating manual at an easily accessible location.

The device may only be transferred with the operating manual.

If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.

1.2Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1	Danger symbols
	Biohazard	Explosion
	Strong magnetic field	UV radiation
	Electric shock	Heavy loads
	Cuts	Hot surface
	Hazard point	Material damage

1.2.2Danger levels

DANGER	Will lead to severe injuries or death.

WARNING	May lead to severe injuries or death.

CAUTION	May lead to light to moderate injuries.

NOTICE	May lead to material damage.

Operating instructions

10epMotion® 5075 English (EN)

1.3Symbols used

Depiction			Meaning

1.			Actions in the specified order
2.

			Actions without a specified order

•			List:

Text			Display or software texts

			Additional information
			Additional information

1.4Abbreviations used

BCR

Barcode reader

DNA

Deoxyribonucleic acid (DNA)

DWP

Deepwell plate

epT.I.P.S.

eppendorf Totally Integrated Pipetting System

HEPA

High-Efficiency Particulate Air (filter)

Liquid handling

MTP

Micro test plate

PCR

Polymerase chain reaction

PDF

Portable Document Format

TMX

Thermomixer

USB

Universal Serial Bus

Ultraviolet radiation

VAC

Vacuum unit

Operating instructions epMotion® 5075 11

English (EN)

1.5Glossary

Application

Programs for a specific application. An application includes the procedure and the equipping of the worktable.

Barcode reader

Device for scanning a barcode.

CleanCap

Optional epMotion equipment. The CleanCap includes a HEPA filter and UV lamp.

Destination labware

Labware that liquid is dispensed into during the application. Destination labware refers to a plate or rack.

Destination position

Position that liquid is dispensed into during the application.

Destination vessel

The vessel into which liquid is dispensed during the application.

Dispensing tool

Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing tools are available for various volume ranges.

epBlue

Software for creating and administering applications and labware. The epMotion is controlled using the software. epBlue is used to manage access to epMotion. epBlue can be expanded. The ID module enables barcode tracking. The GxP module enables electronic documentation in support of 21 CFR Part 11.

Eppendorf Quality

Eppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the requirements for standard products, e.g., precision, accuracy, wetting behavior and tightness.

epT.I.P.S.® Motion

epMotion pipette tips. Only epT.I.P.S.® Motion can be used on the epMotion for all features. epT.I.P.S.® Motions are available with or without a filter.

Operating instructions

12epMotion® 5075 English (EN)

Filling volume

Maximum liquid volume of a labware. The epMotion uses the gripper to transport the labware up to the filling volume. The epMotion aspirates liquid from the labware up to the filling volume. The filling volume is higher than the working volume.

Gripper

Tool that transports labware.

Height adapter

Adapter for low labware. The height differences among the labware will be offset to decrease the tool holder paths, thereby decreasing the run time of the application as well.

HEPA filter

Air filter. The air filter prevents particles such as dust and germs from the environment entering the epMotion work room.

Intermediate labware

Labware that is used to dispense and aspirate liquids during the application.

Intermediate position

Position that is used to dispense and aspirate liquids during the application.

Intermediate vessel

Vessel that is used to dispense and aspirate liquids during the application.

Labware

Racks, plates, tips, etc. which are placed on the worktable.

Location

Area on the worktable where the labware is placed.

MagSep

System consisting of the magnetic finger module, thermomixer and PrepRack and reagents for nucleic acid preparation with the epMotion.

MultiCon

epMotion operator panel. You can control your epMotion using the control unit and the epBlue software.

Operating instructions epMotion® 5075 13

English (EN)

PCR clean

PCR clean is an Eppendorf AG purity grade for consumables. PCR clean meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. PCR clean also meets the requirements with regard to absence of human DNA, DNase, RNase and PCR inhibitors. Consumables with the PCR clean purity grade are controlled and certified by an external laboratory. Certificates are available for downloading from our webpage www.eppendorf.com.

Procedure

Sequence of commands that are executed one after the other. Part of an application.

Rack

Mount for tubes or pipette tips.

Random error

Precision. Standard deviation of the average value of the dispensed volumes.

Remaining volume

Volume that cannot be aspirated from a vessel. The distance from the pipette tip to the vessel bottom, defined in the software, and the defined immersion depth of the pipette tip in the liquid, must be observed. Therefore, the pipette tip cannot aspirate the volume. The remaining volume depends on the vessel geometry.

Reservoir

Reservoirs are used to hold reagents. Reservoirs are hung in a Reservoir Rack or placed directly on the worktable.

SafeRack

Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination of adjacent tips. Use the SafeRacks if you would like to use tips several times.

Source labware

Labware that liquid is aspirated from during the application. Source labware refers to a plate or rack.

Source position

Position that liquid is aspirated from during the application.

Source vessel

The vessel from which liquid is aspirated during the application.

Sterile

Sterile is an Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. Sterile also meets the requirements with regard to sterility and freedom from pyrogens.

Operating instructions

14epMotion® 5075 English (EN)

Systematic error

Accuracy. Deviation of the average value of the dispensed volumes from the selected volume.

Thermal module

Thermal module for labware that is integrated in the worktable.

Thermoadapter

Heat-conductive adapter for holding plates.

Thermoblock

A thermoadapter that is permanently connected to a PCR plate or PCR tube.

Thermorack

Temperable rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.

Vacuum unit

The vacuum unit uses a vacuum to suck liquid from a filter plate. The vacuum unit consists of a vacuum manifold, vacuum pump and Vac Frame.

Vessel

Tube or single well of a plate.

Work volume

Liquid volume of a labware. The epMotion fills a vessel with low levels of contamination up to the working volume. The working volume is less than the filling volume.

Worktable

Work surface of the epMotion where labware and tools are placed. In the software, the epMotion worktable is shown as the epBlue worktable.

Operating instructions	15
epMotion® 5075	15
English (EN)

1.6Revision history

Revision history of the epMotion 5075 hardware operating manual, order no. 5075 900.840.

Version of the operating manual	Date	Changes

Version 00	June 2013	Created new

Operating instructions

16epMotion® 5075 English (EN)

Product description epMotion® 5075 17

English (EN)

2Product description

2.1Main illustration

The epMotion 5075 is available with various features. Details of the worktable for your device (see

Worktable on p. 33).

2.1.1epMotion 5075

1 2

epMotion	5075

Fig. 2-1: Main illustration epMotion 5075I

1	Carrier	6
	The carrier moves in X-direction, Y-direction and
	Z-direction.
2	Tool holder	7
	Holds dispensing tools.
3	Optical sensor	8
	Detects levels, tips and labware.	9
		9
4	CleanCap (optional)	10
	contains HEPA filter and UV lamps	10
	contains HEPA filter and UV lamps

5Worktable

Work surface for tools and labware.

Front hood

Safety device for protection from movable, UV radiation parts and contamination.

Thermal module (optional)

Plate for heating and cooling labware.

Mains power switch

Mouse

MultiCon

Control unit used to control the epMotion.

Product description

18epMotion® 5075 English (EN)

2.1.2 Interfaces

1 2

Fig. 2-2: Interfaces epMotion 5075
1 Ethernet	3 Mains power connection
Connection for the cable to the MultiCon

2USB

Connection for a USB storage medium for firmware updates.

Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected to the interfaces.

2.1.3MultiCon

Information can be found in the operating manual of the MultiCon.

Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected to the interfaces.

Product description epMotion® 5075 19

English (EN)

2.2Delivery package

The epMotion is available in various versions. Your device has been equipped according to your specifications. For some software modules additional accessories are available.

2.2.1epMotion 5075l and epMotion 5075lg

Quantity	Order no.	Description
	(International)

		Automated pipetting system epMotion 5075l
		main device; with MultiCon, keyboard, mouse,
		installed software epBlue
1	5075 000.271	100 V - 240 V, 50 / 60 Hz

		Automated pipetting system epMotion 5075lg
		main device; with MultiCon, keyboard, mouse,
		installed software epBlue GxP
or 1	5075 000.275	100 V - 240 V, 50 / 60 Hz

2.2.2epMotion 5075t and epMotion 5075tg

Quantity	Order no.	Description
	(International)

		Automated pipetting system epMotion 5075t
		main device with thermomixer; with MultiCon, keyboard, mouse,
		installed software epBlue
1	5075 000.272	100 V - 240 V, 50 / 60 Hz

		Automated pipetting system epMotion 5075tg
		main device with thermomixer; with MultiCon, keyboard, mouse,
		installed software epBlue GxP
or 1	5075 000.276	100 V - 240 V, 50 / 60 Hz

2.2.3epMotion 5075v and epMotion 5075vg

Quantity	Order no.	Description
	(International)

		Automated pipetting system epMotion 5075v
		main device with vacuum unit; with MultiCon, keyboard, mouse,
		installed software epBlue
1	5075 000.273	100 V - 240 V, 50 / 60 Hz

		Automated pipetting system epMotion 5075vg
		main device with vacuum unit; with MultiCon, keyboard, mouse,
		installed software epBlue GxP
or 1	5075 000.277	100 V - 240 V, 50 / 60 Hz

1	–	Reservoir 400 mL

		Vac Frame 2
1	5075 785.005	vacuum frame

		Vac Holder
1	5075 778.009	adapter for Vac Frame

1	5282 000.018	Gripper

Product description

20epMotion® 5075 English (EN)

2.2.4 epMotion 5075vt and epMotion 5075vtg

Quantity	Order no.	Description
	(International)

		Automated pipetting system epMotion 5075vt
		main device with vacuum unit and thermomixer; with MultiCon,
		keyboard, mouse, installed software epBlue
1	5075 000.274	100 V - 240 V, 50 / 60 Hz

		Automated pipetting system epMotion 5075vtg
		main device with vacuum item and thermomixer; with MultiCon,
		keyboard, mouse, installed software epBlue GxP
or 1	5075 000.278	100 V - 240 V, 50 / 60 Hz

1	–	Reservoir 400 mL

		Vac Frame 2
1	5075 785.005	vacuum frame

		Vac Holder
1	5075 778.009	adapter for Vac Frame

1	5282 000.018	Gripper

2.2.5epMotion 5075m and epMotion 5075mg

Quantity	Order no.	Description
	(International)

		Automated pipetting system epMotion 5075m
		main device with magnet finger module and thermomixer; with
		MultiCon, keyboard, mouse, installed software epBlue
1	5075 000.270	100 V - 240 V, 50 / 60 Hz

		Automated pipetting system epMotion 5075mg
		main device with magnet finger module and thermomixer; with
		MultiCon, keyboard, mouse, installed software epBlue GxP
or 1	5075 000.279	100 V - 240 V, 50 / 60 Hz

Product description	21
epMotion® 5075	21
English (EN)

2.2.6Accessories for all versions of the epMotion

Quantity	Order no.	Description
	(International)

		Mouse
1	-	With USB connection cable

		Keyboard
1	-	With USB connection cable

		Cable
1	-	For connecting the MultiCon to the epMotion

		Mains power cable
1	-	Compatible with the country where the order was placed or defined

		USB-Ethernet adapter
1	-	For connecting the MultiCon to a network

1	-	Tool for transport securing device

1	5075 753.006	Waste box

		Operating Manual
1	5075 900.840	epMotion 5075
1	5073 900.866	software operating manual epBlue with MultiCon

		CleanCap
optional 1	5075 751.607	with HEPA air filter and UV-lamp

		UV tube
optional 2	-	For epMotion with CleanCap

2.2.7Accessories for epMotion with epBlue ID

Quantity	Order no.	Description
	(International)

		USB dongle eLicenser
1	–	with hardware key code card

		Barcode reader
1		With USB connection cable and table stand

2.2.8Accessories for epMotion with epBlue GxP

Quantity	Order no.	Description
	(International)

		USB dongle eLicenser
1	–	with hardware key code card

		Documentation folder
1	–	Folder for certificates and documentation

		Operating Manual
1	5075 900.874	epBlue GxP

Product description

22epMotion® 5075 English (EN)

2.3Features

The epMotion 5075 is available with different features. All available features are described in this manual.

2.3.1Function

The epMotion 5075 is an automatic system for dispensing liquids using dispensing tools and ep.T.I.P.S. Motion pipette tips.

The epMotion 5075 dispenses liquids in the volume range 1 μL – 1 000 μL.

The dispensing tool aspirates liquid from source vessels and dispenses this liquid in destination vessels. The dispensing tool works according to the piston-stroke principle. For the epMotion, dispensing tools are available for 3 different volume ranges.

The epMotion features an optical sensor. The sensor checks:

•Type and location of labware

•Quantity and position of pipette tips in the rack

•Filling level of vessels

You control the epMotion 5075 via the MultiCon control unit with the epBlue software.

You can use the epBlue software to define dispensing processes and compile them into an application. To do so, you select source vessels and destination vessels, define the procedure and define the transfer pattern.

2.3.2Gripper

A gripper is available for the epMotion. The gripper stacks and transports labware.

2.3.3Thermal module

The epMotion can be equipped with a maximum of 3 thermal modules.

2.3.4Thermomixer

The epMotion can be equipped with a thermomixer.

Product description epMotion® 5075 23

English (EN)

2.3.5Magnetic separation

The epMotion can be equipped with a thermomixer and a magnet finger module for separating magnetic particles.

This epMotion is a system for preparing nucleic acids. MagSep reagent kits are available for this system.

2.3.6Vacuum unit

The epMotion can be equipped with a vacuum unit.

With this unit, the epMotion can perform a fully automated nucleic acid purification.

2.3.7CleanCap

As an option, the epMotion can be equipped with a CleanCap.

The CleanCap contains a UV lamp and a HEPA filter.

Additional information on your epMotion is available at: www.eppendorf.com/automation.

Product description

24epMotion® 5075 English (EN)

Safety	25
epMotion® 5075	25
English (EN)

3Safety

3.1Intended use

3.1.1Intended use of the epMotion 5075

The device is intended for use in laboratories for research, development, and industrial and routine work, as well as for training purposes. The areas of applications include – but are not limited to – the fields of life sciences, biotechnology, chemistry and clinical research.

All epMotion 5075 (l, t, v, vt, m) pipetting systems are designed for the monitoring of contamination-free, precise and correct dispensing and transferring of liquids and to automatically check the combining of liquids.

The autoclavable dispensing tools work in a volume range from 1 μL to 1000 μL.

The epMotion5075v with integrated vacuum station, for instance, performs fully automated nucleic acid purification.

The epMotion 5075m with an integrated thermomixer is used for applications which allow mixing and incubating with open tubes and it performs fully automated nucleic acid purification with magnetic particles.

The epMotion 5075t with an integrated thermomixer is used for applications which allow mixing and incubating with open tubes.

The device meets the basic, essential requirements of the EC Directives and Standards that are listed in the declaration of conformity, but the product is not registered with the FDA.

These automated pipetting systems are exclusively intended for use indoors and may only be used by skilled personnel who have received adequate training.

3.2User profile

This device may only be operated by skilled personnel.

The skilled personnel must have received training on this device. The training must have been conducted by Eppendorf AG or an authorized partner of Eppendorf AG.

The skilled personnel must have read the operating manual carefully. The skilled personnel must have read the operating manuals of all software components carefully.

Safety

26epMotion® 5075 English (EN)

3.3Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

•The device is not used in accordance with the operating manual.

•The device is used outside of its intended use.

•The device is used with accessories or consumables which are not recommended by Eppendorf.

•The device is maintained or repaired by people not authorized by Eppendorf.

•The user makes unauthorized changes to the device.

3.4Warnings for intended use

DANGER! Risk of explosion.

Do not operate the device in areas where work is completed with explosive substances.

Do not use this device to process any explosive or highly reactive substances.

Do not use this device to process any substances which may generate an explosive atmosphere.

DANGER! Damage to health due to escaping aerosols.

When working with substances that can be hazardous to health, harmful aerosols may form and escape from the vacuum pump.

Contact Eppendorf AG before using this type of substances.

WARNING! Lethal voltages inside the device.

Ensure that the housing is always closed and undamaged so that no parts inside the device can be contacted by accident.

Do not remove the housing of the device.

Do not allow any liquids to penetrate the inside of the housing.

Do not allow the device to be opened by anyone except service personnel who have been specifically authorized by Eppendorf.

WARNING! Electric shock due to damage to device or mains cable.

Only switch on the device if the device and mains cable are undamaged.

Only use devices that have been properly installed or repaired.

In case of danger, disconnect the device from the mains supply by pulling the power plug from the device or the mains socket or, by using the isolating device intended for this purpose (e.g. emergency stop switch in the laboratory).

Safety	27
epMotion® 5075	27
English (EN)

WARNING! Risk from incorrect supply voltage

Only connect the device to voltage sources which correspond to the electrical requirements on the name plate.

Only use sockets with a protective earth (PE) conductor and suitable power cable.

WARNING! Damage to health due to infectious liquids and pathogenic germs.

When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

Wear personal protective equipment.

For full instructions regarding the handling of germs or biological material of risk group II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual).

WARNING! Health hazard from skin contact with infectious substances.

Wear protective gloves.

WARNING! Danger due to flammable and infectious liquids in the waste container.

Pipette tips in the waste container may contain flammable or infectious liquids.

Wear personal protective equipment.

Handle the pipette tips and sample materials from the waste container in accordance with the material safety data sheets, safety regulations and laboratory guidelines.

WARNING! Damage to health due to contaminated device and accessories.

Decontaminate the device and the accessories before storage and shipping.

CAUTION! Risk of injury due to carrier movement.

The carrier may still be moving when the front hood of the epMotion is opened.

Wait until the carrier stops moving before reaching into the device.

CAUTION! Cuts due to broken glass.

A damaged touchscreen leads to cuts on the hands.

Only use the MultiCon if it is not damaged.

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.

Only use accessories and original spare parts recommended by Eppendorf.

Safety

28epMotion® 5075 English (EN)

NOTICE! Spilled liquid can cause damage to the device.

Switch off the device.

Disconnect the power plug.

Collect the spilled liquid. Observe the specifications for the liquid in the material safety data sheets.

NOTICE! Size of disposables can change through autoclaving.

Do not use autoclaved disposable products in automated applications.

NOTICE! Data loss and malfunction due to malicious software.

Malicious software (e.g., a computer virus) on the USB storage medium can be transferred to the MultiCon.

Before connecting the USB storage medium to the MultiCon, check the USB storage medium using a current antivirus software.

NOTICE! Data loss due to missing data backup or incorrect storage of storage media.

User accounts, applications, labware and protocols are saved in a database. If this database becomes damaged (e.g., due to a hardware defect), this information will be lost. Eppendorf is not liable for data loss or any associated damages.

Regularly backup your database using the backup function in the software.

Save the secured data on an external storage medium.

Store the external storage media according to the manufacturer’s specifications.

NOTICE! Malfunction due to third-party software.

Third-party software can impair the functioning of the epBlue software.

Only install software approved by Eppendorf.

Safety	29
epMotion® 5075	29
English (EN)

3.5Hazard symbols and safety devices on the device

This section explains the warning symbols on the epMotion and the location of the safety devices.

epMotion	U5075

Fig. 3-1: Hazard symbols on the epMotion 5075

Tab. 3-1: Hazard symbols

1		WARNING
		Observe the operating manual.

2		WARNING
		The carrier may continue moving after the front panel has
		been opened.
		Wait until the carrier stops moving before reaching into
		the device.

Safety

30epMotion® 5075 English (EN)

3		DANGER
		Risk of burns from the hot surface.
		Wait until the surface has cooled before touching it.

5		NOTICE
		Danger due to UV radiation.
		Wear personal protective equipment.

6		The label is located on the magnetic finger module and is
		only visible to the service technician.
		WARNING
		Danger due to strong magnetic field.
		Do not work on the device if you wear a pacemaker.

Tab. 3-2: Safety device

4		The housing protects the user from UV radiation, magnetic
		fields, contamination and movable parts.

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