Eppendorf 5810 R User Manual

Centrifuge 5804/5804 R/5810/5810 R

Operating manual Manuel d'utilisation

Copyright © 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner.

Trademarks

eppendorf and CombiSlide are registered trademarks of Eppendorf AG, Hamburg, Germany. Centriplus is a registered trademark of Millipore Corporation, Billerica, USA.

Microtainer is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or ® in this manual.

Operating manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Manuel d'utilisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139 Declarations and Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267

5820 900.040-02/122012

You will find a detailed description of these figures in your language in Chapters 2.1 and 5.1.

1				Centrifuge lid				2	Monitoring glass
3				Control panel with display				4	Emergency release

5Condensation water tray (only Centrifuge 5804 R/ 5810 R)

Task/function

Press

Display

Details

Set parameter

1.

Press

or

etc.

1.

Selected parameter

Chapter 5.4.1

flashes.

2.

Press

or

.

2.

New value appears.

Soft start/stop

1.

Press

repeatedly.

: Acceleration ramp 0

Chapter 6.2 (English,

(long) ... 9 (short).

German)

2.

Press

or

to

: Deceleration ramp 0

(long) ... 9 (short).

select ramp.

Alarm ON/OFF

Press

+

Alarm on/Alarm off

Chapter 6.7.2 (English,

German)

simultaneously.

Programming

1.

Set parameter.

1.

Parameter.

Chapters 6.4 - 6.6 (English,

(during rotor stop only)

2.

Press 2 ×

.

2.

P...: first idle program no.

German)

3.

OK

3.

Store: Press

> 2 s.

At set rpm

Chapter 6.3 (English,

(with open centrifuge lid

Press

> 4 s.

German)

: on/

: off

only)

1			Short spin centrifugation																																															2					Display
3			Status At set rpm function																																															4					Indicate speed (rpm), g-force (rcf)																																				and radius
																																																							setting .
5			Symbol for acceleration																											and braking																				6					Set parameters and values
7			Release centrifuge lid																																															8					Start or stop centrifugation
9			Adjust centrifugation time																																															10					Set centrifugation speed
11			Select or save program																																															12					Standby
13			Set temperature (only 5804 R/5810 R)																																															14					Start temperature control run FastTemp
																																																							(only 5804 R/5810 R)

1

Program number

2

Symbol for g-force (rcf)

3

g-force (rcf)/rotational speed (rpm)

4

Symbol flashes when rotor is in motion

5

Symbol for acceleration

and braking

6

Centrifugation time

7

Temperature (only 5804 R/5810 R)

Fixed-angle

Angle of

of

Swing-bucket

of

rotor

bores

bores (mm)

rotor

buckets (mm)

Aerosol-tight

Number of

Number of

version

bores

buckets

Fixed-angle

Angle of

of

Swing-bucket

of

rotor

bores

bores (mm)

rotor

buckets (mm)

81

Aerosol-tight	Number of	Number of
version	bores	buckets

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

pTabFigonseeCentrifugeOperating.—... manual5804/5804 R/5810/5810 R

manual Table of contents

1	Table of contents

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.4 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.4.1 Rotor A-4-81 (only 5810/5810 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.4.2 Rotor A-4-62 and A-4-62-MTP (only 5810/5810 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 2.4.3 Rotor A-4-44. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 2.4.4 Rotor A-2-DWP-AT (only 5810/5810 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 2.4.5 Rotor A-2-DWP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 2.4.6 Rotor FA-45-6-30 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 2.4.7 Rotor F-34-6-38 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 2.4.8 Rotors FA-45-30-11 and F-45-30-11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 2.4.9 Rotor F-45-48-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 2.4.10 Rotor T-60-11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 2.4.11 Rotor S-4-104 (only 5810/5810 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 2.4.12 Rotor S-4-72. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 2.4.13 Rotor F-35-48-17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 2.4.14 Rotor FA-45-48-11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 2.4.15 Rotor FA-45-20-17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 3.3 Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 3.3.1 Declaration concerning the ATEX directive (94/9/EC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 3.5.2 Incorrect handling of the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3.5.4 Extreme strain on the centrifuging tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

3.6 Safety instructions located on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 4.2 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 4.3 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5

EN

manual Operating

EN

Operating manual

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 5.2 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 5.2.1 Switching on the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 5.2.2 Inserting the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 5.2.3 Automatic rotor detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 5.2.4 Loading the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 5.2.5 Closing the centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

5.3 Cooling (only 5804 R/5810 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 5.3.1 Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 5.3.2 Temperature display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 5.3.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 5.3.4 FastTemp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 5.3.5 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

5.4 Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 5.4.1 Centrifuging with preset time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 5.4.2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 5.4.3 Short spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 5.4.4 Removing the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 5.4.5 Standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6 Operating controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

6.1 Setting the radius . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 6.2 Setting the acceleration and braking times. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 6.3 Setting the start of run time (At set rpm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 6.4 Saving the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 6.5 Loading the program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 6.6 Deleting the program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 6.7 Special functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.7.1 Display operating hours . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 6.7.2 Switching on/off the warning signal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 6.7.3 Exiting the service functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 6.7.4 Controlling the centrifuge via the serial interface (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7.1 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 7.2 Prepare cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 7.3 Cleaning/disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 7.3.1 Cleaning and disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 7.3.2 Cleaning and disinfecting the rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

7.4 Additional service instructions for Centrifuge 5804 R/5810 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 7.5 Glass breakage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 7.6 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

8.1 Resetting the excess current switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 8.2 General errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 8.3 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67 8.4 Emergency release. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

9.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 9.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 9.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

10 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

10.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 10.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 10.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 10.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

11 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

11.1 Centrifuge 5804/5804 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 11.2 Centrifuge 5810/5810 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 11.3 Rotors and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 11.3.1 Rotor A-4-81 (only 5810/5810 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74 11.3.2 Rotor A-4-62 and A-4-62-MTP (only 5810/5810 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76 11.3.3 Rotor A-4-44. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 11.3.4 Rotor A-2-DWP-AT (only 5810/5810 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 11.3.5 Rotor A-2-DWP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 11.3.6 Rotor FA-45-6-30 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79 11.3.7 Rotor F-34-6-38 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79 11.3.8 Rotor FA-45-30-11 and rotor F-45-30-11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 11.3.9 Rotor F-45-48-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 11.3.10 Rotor T-60-11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 11.3.11 Rotor S-4-104. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 11.3.12 Rotor S-4-72. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 11.3.13 Rotor F-35-48-17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 11.3.14 FA-45-48-11 rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 11.3.15 Rotor FA-45-20-17 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 11.3.16 Miscellaneous . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

7

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

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Operating manual

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

1User instructions

1User instructions

1.1Using this manual

Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable.

This operating manual is part of the product. Thus, it must always be easily accessible.

Enclose this operating manual when transferring the device to third parties.

If this manual is lost, please request another one. You will find the current version on our webpage www.eppendorf.com/worldwide.

1.2Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1Danger symbols

Biohazard	Explosion
Electric shock	Crushing
Hazard point	Material damage

1.2.2Danger levels

DANGER	Will lead to severe injuries or death.
WARNING	May lead to severe injuries or death.
CAUTION	May lead to light to moderate injuries.
NOTICE	May lead to material damage.

1.3Symbols used

Example Meaning

You are requested to perform an action.

	1.			Perform these actions in the sequence described.
	2.
			•	List.
				Press this key to perform the described action.
	Text			Terms from the display of the device.
				References useful information.

9

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Operating manual

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

1.4Abbreviations used

MTP Micro test plate

NN Mean sea level (MSL) PCR Polymerase chain reaction PTFE Polytetrafluorethylene

RCF Relative centrifugal force – g-force in m/s2 rpm Revolutions per minute – in rpm

UV Ultraviolet radiation

10

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

2 Product description

2	Product description

2.1Main illustration

Abb. 1:

Depiction of Centrifuge 5810 and Centrifuge 5810 R. The Centrifuge 5804 and Centrifuge 5804 R are similar in design.

EN

manual Operating

Fig. 1: Depiction of Centrifuge 5810 and Centrifuge 5810 R. The Centrifuge 5804 and Centrifuge 5804 R are similar in design.

1	Centrifuge lid	2	Monitoring glass
			Visual control for rotor stop or option for speed check
			via stroboscope
3	Operator panel with display	4	Emergency release
	(see Overview of operating controls on p. 50)		(see Emergency release on p. 69)
5	Condensation water tray (only Centrifuge 5804 R/
	5810 R)

The depiction of the centrifuge can be found on the front fold-out page (see Fig. 1).

2.2Delivery package

Each delivery package consists of one of the following centrifuges and the accessories listed in the following.

Quantity	Order no.	Order No.	Description
	(international)	(North America)
			5804/5804 R/5810/5810 R centrifuge
			See chapter Ordering Information for corresponding device version,
1	-	-	equipment and order number
			Rotor key
1	5810 350.018	022664166	Standard
			Power cable
			see chapter Ordering information for corresponding power cable
1	-	-	variants and order numbers

11

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Operating manual

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Quantity	Order no.	Order No.	Description
	(international)	(North America)
			Operating manual
			Centrifuge 5804/5804 R/5810/5810 R
1	5820 900.040	5820900040	Languages: EN, DE, FR, ES, IT, PT
			Operating manual
			Centrifuge 5804/5804 R/5810/5810 R
1	5820 900.059	5820900059	Languages: DA, EL, FI, NL, SV (230 V devices only)
			Test log book
1	5820 901.004	-	(for Germany only)
	Only 5804 R and 5810 R:
Quantity	Order no.	Order No.	Description
	(international)	(North America)
1	5811 001.068	022662678	Tray for condensation water

2.3Features

The versatile 5804/5804 R/5810/5810 R centrifuge has a capacity of maximally 4 × 250 mL (5804/5804 R centrifuge) resp. 4 × 750 mL (5810/5810 R centrifuge) and achieves max. 20,800 × g/14,000 rpm. The versatility is reflected in the available rotor options. You can select between 12 (5804/5804 R centrifuge) resp. 16 (5810/5810 R centrifuge) different rotors to centrifuge the following tubes for your various applications:

•Micro test tubes (0.2 to 5.0 mL)

•PCR strips

•Microtainers

•Spin columns

•Cryotubes

•Conical tubes (15/50 mL)

•Bottles (175 to 750 mL)

•Various tubes (3 to 120 mL)

•Microplates

•PCR plates

•Deepwell plates (max. height 29 mm)

•Slides (with CombiSlide adapter)

•Cell culture tubes

Handling the centrifuge is facilitated by:

•Low access height of 29 cm for loading and unloading the rotors

•Automatic rotor detection with rotational speed limit

•Automatic rotor imbalance detection

•Clear digital display

All centrifuges in these series have 35 program spaces for user-defined settings and 10 different acceleration and braking ramps.

Adapter-specific manual radius adjustment guarantees maximum RCF accuracy.

The Centrifuge 5804 R/5810 R has an additional temperature control function for centrifugation between -9°C and 40°C. Use the FastTemp function to start a temperature control run without samples to adjust the rotor chamber incl. rotor, buckets and adapters quickly to the set target temperature. Continuous cooling also maintains the temperature in the rotor chamber with the centrifuge lid closed when the centrifuge is not in use.

The built-in condensation drain eliminates water accumulation and prevents corrosion.

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

2.4Rotors

Eppendorf centrifuges may only be operated with rotors that are intended for use with the Hint! corresponding centrifuge.

Only use rotors which bear the name of the centrifuge (e.g. 5804 R).

You can operate the 5804/5804 R/5810/5810 R centrifuge with the following rotors. Before use of sample tubes, please note the manufacturer's specifications with regard to centrifugation resistance (max. rcf).

2.4.1Rotor A-4-81 (only 5810/5810 R)

Rotor A-4-81 with 500 mL rectangular bucket

				Max. g-force:	3,220 × g

Rotor A-4-81	Rectangular bucket Aerosol-tight cap	Max. speed:	4,000 rpm
	500 mL
Swing-bucket rotor		Max. load per bucket	780 g
with 4 × 500 mL		(adapter, tube and
rectangular buckets		contents):

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Vessel	Vessel	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with/without
			aerosol-tight
			bucket cap
	Vessel		flat	2,950 × g
	1.5/2 mL		Ø 11 mm	4,000 rpm
	20/80	5810 745.004	43 mm/43 mm	16.5 cm
	Blood collection tube		flat	3,000 × g
	1.2 to 5 mL		Ø 11 mm	4,000 rpm
	20/80	5810 746.000	108 mm/108 mm	16.8 cm
	Vessel		flat	3,000 × g
	2.6 to 5 mL		Ø 13 mm	4,000 rpm
	25/100	5810 720.001	107 mm/108 mm	16.8 cm
	Vessel		flat	3,000 × g
	2.6 to 7 mL		Ø 13 mm	4,000 rpm
	18/72	5810 747.007	108 mm/108 mm	16.8 cm

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

EN

Vessel

Operating manual

Eppendorf

Vessel	Adapters	Adapter bottom	Max. g-force
		shape
Capacity	Order no.	Tube diameter	Max. speed
	(international)
Vessels per		Max. tube length	Centrifugation radius
adapter/rotor		with/without
		aerosol-tight
		bucket cap
Blood collection tube		flat	3,000 × g
3 to 15 mL		Ø 16 mm	4,000 rpm
16/64	5810 748.003	108 mm/108 mm	16.8 cm
Vessel		flat	3,000 × g
7 to 17 mL		Ø 17.5 mm	4,000 rpm
16/64	5810 721.008	118 mm/118 mm	16.8 cm
Conical tube		conical	3,100 × g
15 mL		Ø 17.5 mm	4,000 rpm
12/48	5810 722.004	119 mm/121 mm	17.3 cm
Conical tube		conical	3,100 × g
50 mL		Ø 31 mm	4,000 rpm
5/20	5810 723.000	116 mm/122 mm	17.3 cm
Centriprep		flat	3,100 × g
50 mL		Ø 31 mm	4,000 rpm
5/20	5810 739.004	(Do not use the	17.3 cm
		aerosol-tight cap.)/
		121 mm
Conical tube, skirted		flat	3,100 × g
50 mL		Ø 31 mm	4,000 rpm
5/20	5810 739.004	(Do not use the	17.3 cm
		aerosol-tight cap.)/
	5804 737.008	119 mm
Bottles		flat	3,100 × g
180 to 250 mL		Ø 62 mm	4,000 rpm
1/4	5825 722.000	(Do not use the	17.3 cm
		aerosol-tight cap.)/
		133 mm
Wide-neck bottle		flat	3,220 × g
400 mL		Ø 81 mm	4,000 rpm
1/4	5810 728.002	(Do not use the	18.0 cm
		aerosol-tight cap.)/
		133 mm

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Vessel	Vessel	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with/without
			aerosol-tight
			bucket cap
	Wide-neck bottle,	-	flat	3,220 × g
Eppendorf	rectangular
	500 mL		83 mm	4,000 rpm
	-/4		134 mm/134 mm	18.0 cm
	Rotor A-4-81 with conical tubes
			Max. g-force:	3,220 × g

Rotor A-4-81		Bucket for	Max. speed:	4,000 rpm
		7 × 50 mL conical
		tubes
Swing-bucket rotor with 4 buckets for			Max. load per bucket	7 × 75 g
conical tubes			(adapter, tube and
			contents):
Vessel	Vessel	Adapters	Bottom shape	Max. g-force
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor
	Conical tube		conical	3,184 × g
	15 mL		Ø 17.5 mm	4,000 rpm
	7/28	5820 718.005	120 mm	17.8 cm
	Conical tube	-	conical	3,220 × g
	50 mL		Ø 30 mm	4,000 rpm
	7/28		117 mm	18.0 cm

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Rotor A-4-81 with MTP/Flex carrier

Max. g-force:

2,900 × g

Rotor A-4-81	MTP/Flex buckets	Max. speed:	4,000 rpm
Swing-bucket rotor with 4 MTP/Flex carriers		Max. load per bucket	380 g
		(adapter, plate and
		contents):

Vessel	Plate	Adapters	Bottom shape	Max. g-force
	Capacity	Order no.	Tube diameter	Max. speed
		(international)

Plates, rack or		Max. loading height	Centrifugation radius
glass slides per
adapter/rotor
Micro test plate	-	flat	2,900 × g
96/384 wells		-	4,000 rpm
4/16		60 mm	16.3 cm
Deepwell plate	-	flat	2,900 × g
96 wells		-	4,000 rpm
1/4		60 mm	16.3 cm
Cell culture plate	-	flat	2,900 × g
		-	4,000 rpm
2/8		60 mm	16.3 cm
Kit	-	flat	2,900 × g
		-	4,000 rpm
1/4		60 mm	16.3 cm
Tube in IsoRack		flat	2,700 × g
24 × 0.5 mL		Ø 6 mm	4,000 rpm
1/4	5825 708.008	60 mm	15.0 cm
Tube in IsoRack		flat	2,600 × g
24 × 1.5/2 mL		Ø 11 mm	4,000 rpm
1/4	5825 709.004	60 mm	14.6 cm

384-well PCR plate		flat	2,700 × g
		-	4,000 rpm
1/4	5825 713.001	60 mm	15.8 cm
96-well PCR plate		flat	2,600 × g
		-	4,000 rpm
1/4	5825 711.009	60 mm	16.1 cm

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Vessel	Plate	Adapters	Bottom shape	Max. g-force
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Plates, rack or		Max. loading height	Centrifugation radius
	glass slides per
	adapter/rotor
Slides	CombiSlide		flat	1,000 × g
	12 slides	5825 706.005	-	2,372 rpm
	12/48		60 mm	15.9 cm
	Cell culture bottle		flat	1,000 × g
	with/without filter
	75 cm2:	5825 719.000	-	2,501 rpm
	Sarstedt
	83.1811.002/
	83.1811
	25 cm2:
	Sarstedt
	83.1810.002/
	83.1810
	Greiner Bio-One
	690175/690160
	TPP 90026/90025
	IWAKI 3102-025
	1/4		60 mm	14.3 cm

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

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2.4.2Rotor A-4-62 and A-4-62-MTP (only 5810/5810 R)

Rotor A-4-62 with 250 mL rectangular bucket

Operating manual

		Max. g-force:	3,220 × g

Rotor A-4-62	Rectangular bucket	Aerosol-tight cap	Max. speed:	4,000 rpm
	250 mL
Swing-bucket rotor			Max. load per bucket	620 g
with 4 × 250 mL			(adapter, tube and
rectangular buckets			contents):
Vessel	Vessel	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with/without
			aerosol-tight
			bucket cap
	Vessel		flat	3,000 × g
	1.5/2 mL		Ø 11 mm	4,000 rpm
	16/64	5810 751.004	43 mm/43 mm	17.1 cm
	Vessels		flat	3,050 × g
	1.2 to 5 mL		Ø 11 mm	4,000 rpm
	25/100	5810 750.008	115 mm/123 mm	17.3 cm
	Vessels		flat	3,050 × g
	2.6 to 7 mL		Ø 13 mm	4,000 rpm
	15/60	5810 752.000	118 mm/121 mm	17.3 cm
	Vessels		flat	3,050 × g
	3 to 15 mL		Ø 16 mm	4,000 rpm
	12/48	5810 753.007	116 mm/121 mm	17.3 cm
	Vessels		flat	3,050 × g
	7 to 17 mL		Ø 17.5 mm	4,000 rpm
	12/48	5810 754.003	114 mm/118 mm	17.3 cm
	Conical tube		conical	3,150 × g
	15 mL		Ø 17.5 mm	4,000 rpm
	9/36	5810 755.000	121 mm/127 mm	17.8 cm

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Vessel	Vessel	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with/without
			aerosol-tight
			bucket cap
	Vessel		flat	3,050 × g
	7 to 18 mL		Ø 20 mm	4,000 rpm
	8/32	5810 756.006	119 mm/126 mm	17.3 cm
	Vessel		flat	3,050 × g
	18 to 30 mL		Ø 26 mm	4,000 rpm
	4/16	5810 757.002	116 mm/119 mm	17.3 cm
	Conical tube		conical	3,150 × g
	50 mL		Ø 31 mm	4,000 rpm
	3/12	5810 758.009	116 mm/122 mm	17.8 cm
	Conical tube		conical	3,050 × g
	50 mL		Ø 31 mm	4,000 rpm
	4/16	5810 763.002	(Do not use the	17.3 cm
			aerosol-tight cap.)/
		5804 728.009	122 mm
	Vessel		flat	3,050 × g
	30 to 50 mL		Ø 31 mm	4,000 rpm
	4/16	5810 759.005	(Do not use the	17.3 cm
			aerosol-tight cap.)/
			119 mm
	Conical tube, skirted		flat	3,050 × g
	50 mL		Ø 31 mm	4,000 rpm
	4/16	5810 759.005	(Do not use the	17.3 cm
			aerosol-tight cap.)/
		5804 737.008	119 mm
	Vessel		flat	3,050 × g
	50 to 75 mL		Ø 35 mm	4,000 rpm
	2/8	5810 760.003	118/122 mm	17.3 cm
	Vessel		flat	3,050 × g
	80 to 120 mL		Ø 45 mm	4,000 rpm
	1/4	5810 761.000	125/138 mm	17.3 cm

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Centrifuge 5804/5804 R/5810/5810 R — Operating manual

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Operating manual

Vessel	Vessel	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with/without
			aerosol-tight
			bucket cap
	Bottles		flat	3,220 × g
	180 to 250 mL		Ø 62 mm	4,000 rpm
	1/4	5810 770.009	127/136 mm	18.0 cm

Rotor A-4-62 with MTP carrier

Max. g-force:

2,750 × g

Rotor A-4-62	MTP buckets	Max. speed:	4,000 rpm
Swing-bucket rotor with 4 MTP buckets		Max. load per bucket	380 g
		(adapter, plate and
		contents)

Plate	Plate	Adapters	Bottom shape	Max. g-force
	Capacity	Order no.		Max. speed
		(international)
	Plates or glass		Max. loading height	Centrifugation radius
	slides per adapter/
	rotor
	Micro test plate		flat	2,750 × g
	96/384 wells			4,000 rpm
	4/16		53 mm	15.4 cm
	Deepwell plate		flat	2,750 × g
	96/384 wells			4,000 rpm
	1/4		53 mm	15.4 cm
	Cell culture plate		flat	2,750 × g
				4,000 rpm
	2/8		53 mm	15.4 cm
	384-well PCR plate		flat	2,700 × g
				4,000 rpm
	1/4	5825 713.001	53 mm	14.9 cm

20

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Plate	Plate	Adapters	Bottom shape	Max. g-force
	Capacity	Order no.		Max. speed
		(international)
	Plates or glass		Max. loading height	Centrifugation radius
	slides per adapter/
	rotor
	96-well PCR plate		flat	2,600 × g
				4,000 rpm
	1/4	5825 711.009	53 mm	15.2 cm
Slides	CombiSlide		flat	1,000 × g
	12 slides	5825 706.005		2,442 rpm
	12/48		53 mm	15.0 cm
	Cell culture bottle		flat	1,000 × g
	75 cm2:	5825 719.000		2,584 rpm
	Sarstedt
	83.1811.002/
	83.1811
	25 cm2:
	Sarstedt
	83.1810.002/
	83.1810
	Greiner Bio-One
	690175/690160
	TPP 90026/90025
	IWAKI 3102-025
	1/4		60 mm	13.4 cm

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21

EN

Operating manual

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

2.4.3Rotor A-4-44

		Max. g-force:	4,400 × g

Rotor A-4-44	Rectangular bucket	Aerosol-tight cap	Max. speed:	5,000 rpm
	100 mL
Swing-bucket rotor			Max. load per bucket	310 g
with 4 × 100 mL			(adapter, tube and
rectangular buckets			contents):
Vessel	Vessel	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with/without
			aerosol-tight
			bucket cap
	Vessel		flat	4,100 × g
	1.5/2 mL		Ø 11 mm	5,000 rpm
	12/48	5804 751.000	43 mm/43 mm	14.8 cm
	Vessels		flat	4,200 × g
	1.2 to 5 mL		Ø 11 mm	5,000 rpm
	14/56	5804 750.004	102 mm/105 mm	15.0 cm
	Vessels		flat	4,200 × g
	2.6 to 7 mL		Ø 13 mm	5,000 rpm
	9/36	5804 752.007	106 mm/108 mm	15.0 cm
	Vessels		flat	4,200 × g
	3 to 15 mL		Ø 16 mm	5,000 rpm
	7/28	5804 753.003	106 mm/108 mm	15.0 cm
	Vessels		flat	4,200 × g
	7 to 17 mL		Ø 17.5 mm	5,000 rpm
	6/24	5804 754.000	106 mm/110 mm	15.0 cm
	Conical tube		conical	4,300 × g
	15 mL		Ø 17.5 mm	5,000 rpm
	4/16	5804 755.006	(Do not use the	15.5 cm
			aerosol-tight cap.)/
			121 mm

22

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Vessel	Vessel	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with/without
			aerosol-tight
			bucket cap
	Conical tube		conical	4,400 × g
	15 mL		Ø 17.5 mm	5,000 rpm
	2/8	5804 717.007	121 mm/121 mm	15.7 cm
	Vessel		flat	4,200 × g
	7 to 18 mL		Ø 20 mm	5,000 rpm
	4/16	5804 756.002	104 mm/107 mm	15.0 cm
	Vessel		flat	4,200 × g
	18 to 30 mL		Ø 26 mm	5,000 rpm
	2/8	5804 757.009	100 mm/110 mm	15.0 cm
	Conical tube		conical	4,300 × g
	50 mL		Ø 31 mm	5,000 rpm
	1/4	5804 758.005	(Do not use the	15.5 cm
			aerosol-tight cap.)/
			122 mm
	Conical tube		conical	4,400 × g
	50 mL		Ø 31 mm	5,000 rpm
	1/4		119 mm/122 mm	15.7 cm
		5804 718.003
	Conical tube		flat with conical	4,500 × g
			insert
	50 mL		-	5,000 rpm
	-/8	5804 706.005	(Do not use the	16.1 cm
			aerosol-tight cap.)/
		Max. load 144 g	120 mm
		(insert, tubes and
		contents)
	Vessel		flat	4,200 × g
	30 to 50 mL		Ø 31 mm	5,000 rpm
	1/4	5804 759.001	108 mm/122 mm	15.0 cm
	Conical tube, skirted		flat	4,200 × g
	50 mL		Ø 31 mm	5,000 rpm
	1/4	5804 759.001	108 mm/122 mm	15.0 cm
		5804 737.008

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manual Operating

23

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

EN

Operating manual

Vessel	Vessel	Adapters			Adapter bottom	Max. g-force
					shape
	Capacity	Order no.			Tube diameter	Max. speed
		(international)
	Vessels per				Max. tube length	Centrifugation radius
	adapter/rotor				with/without
					aerosol-tight
					bucket cap
	Vessel				flat	4,200 × g
	50 to 75 mL				Ø 35 mm	5,000 rpm
	1/4				108 mm/119 mm	15.0 cm
		5804 760.000
	Vessel				flat	4,200 × g
	80 to 100 mL				Ø 45 mm	5,000 rpm
	1/4	5804 761.006			100 mm/114 mm	15.0 cm

2.4.4Rotor A-2-DWP-AT (only 5810/5810 R)

Max. g-force:

3,486 × g

Rotor A-2-DWP	Bucket	Aerosol-tight cap	Max. speed:	4,500 rpm
Swing-bucket rotor	(always use with		Max. load per bucket	500 g
with 2 aerosol-tight	plate carrier)		(adapter, plate and
buckets			contents):
Plate	Plate	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.		Max. speed
		(international)
	Plate/ slide per		Max. loading height	Centrifugation radius
	adapter/rotor
	Micro test plate	-		3,486 × g
	96/384 wells			4,500 rpm
	4/16		60 mm	154 mm
	Cell culture plate	-		3,486 × g
				4,500 rpm
	2/8		60 mm	154 mm
	Deepwell plate		flat	3,486 × g
	96 mL			4,500 rpm
	1/4		67 mm	154 mm

24

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Plate	Plate	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.		Max. speed
		(international)
	Plate/ slide per		Max. loading height	Centrifugation radius
	adapter/rotor
	Kit	-		3,486 × g
				4,500 rpm
	1/4		60 mm	154 mm
	IsoRack		open	2,500 × g
	24 × 0.5 mL micro		Ø 6 mm	3,900 rpm
	test tubes
	1/4		60 mm	147 mm
	IsoRack		open	2,432 × g
	24 × 1,5/2,0 mL		Ø 11 mm	3.900 g
	micro test tubes
	1/4		60 mm	143 mm
	PCR plate			3,486 × g
	384 wells			4,500 rpm
	1/4	5825 713.001	60 mm	149 mm
	PCR plate			3,486 × g
	96 wells			4,500 rpm
	1/4	5825 711.009	60 mm	154 mm
Slides	CombiSlide		flat	100 × g
	8 slides	5825 706.005		772 rpm
	8/16		60 mm	150 mm

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manual Operating

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EN

Operating manual

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

2.4.5Rotor A-2-DWP

Check the load if using two fully loaded DWP plates.

Hint!

Max. g-force:

2,250 × g

Rotor A-2-DWP		Deepwell plate	Max. speed:	3,700 rpm
		carrier
Swing-bucket rotor with 2 Deepwell plate			Max. load per bucket	380 g
carriers			(adapter, plate and
			contents):
Plate	Plate	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.		Max. speed
		(international)
	Plates/slides per		Max. loading height	Centrifugation radius
	adapter/rotor
	Micro test plate	SBS adapter*	flat	2,250 × g
	96/384 wells	5825 718.003		4,000 rpm
	4/8		89 mm	14.7 cm
	Cell culture plate	SBS adapter*	flat	2,250 × g
		5825 718.003		4,000 rpm
	4/8		89 mm	14.7 cm
	Deepwell plate	SBS adapter*	flat	2,250 × g
	96 wells	5825 718.003		4,000 rpm
	2/4		89 mm	14.7 cm
	Kit	SBS adapter*	flat	2,250 × g
		5825 718.003		4,000 rpm

1/2		89 mm	14.7 cm
Tube in IsoRack		flat	2,050 × g
24 × 0.5 mL		Ø 6 mm	3,700 rpm
1/2	5825 708.008	89 mm	13.8 cm

Tube in IsoRack	flat	1,990 × g
24 × 1.5/2 mL	Ø 11 mm	3,700 rpm
1/2	89 mm	13.3 cm
	5825 709.004

26

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Plate	Plate	Adapters	Adapter bottom	Max. g-force
			shape
	Capacity	Order no.		Max. speed
		(international)
	Plates/slides per		Max. loading height	Centrifugation radius
	adapter/rotor
	384-well PCR plate		flat	2,170 × g
				3,700 rpm
	1/2	5825 713.001	89 mm	14.2 cm
	96-well PCR plate		flat	2,220 × g
				3,700 rpm
	1/2	5825 711.009	89 mm	14.5 cm
Slides	CombiSlide		flat	100 × g
	8 slides	5825 706.005		791 rpm
	8/16		60 mm	14.3 cm

*) Optional. Secures the plate against slipping .

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Operating manual

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

2.4.6Rotor FA-45-6-30

	Rotor FA-45-6-30	Max. g-force:	16,639 × g (5810 R: 20,133 × g)
		Max. speed:	11,000 rpm (5810 R: 12,100 rpm)
	Fixed-angle rotor for
		Max. load (adapter,	6 × 75 g
	6 conical tubes
		tube and contents):
Vessel	Vessel	Adapters	Adapter bottom	Max. g-force at 11,000 rpm
			shape	(5804/5804 R/5810)
	Capacity	Order no.	Tube diameter	Max. g-force at 12,100 rpm
		(international)		(5810 R)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with rotor lid
	Conical tube		conical	16,233 × g
	15 mL		Ø 17 mm	19,642 × g
	1/6	5820 717.009	125 mm	12.0 cm
	Conical tube	-	conical	16,639 × g
	50 mL		Ø 30 mm	20,133 × g
	1/6		127 mm	12.3 cm
	Oak Ridge		Round	16,233 × g
	16 mL		Ø 18.1 mm	19,642 × g
	1/6	5820 720.000	107 mm	12.0 cm
	Oak Ridge		Round	14,204 × g
	30 mL		Ø 25.7 mm	17,187 × g
	1/6	5820 721.006	104 mm	10.5 cm
	Oak Ridge		conical	15,151 × g
	35 mL		Ø 28.7 mm	18,333 × g
	1/6	5820 722.002	113 mm	11.2 cm
	Vessel		conical	16,369 × g
	5 mL		Ø 17 mm	19,806 × g
	1/6	5820 730.005	-	12.1 cm
	Vessel		Round	16,233 x g
	2,6 to 5 mL		Ø 13,5 mm	19,246 × g
	1/6	5820 726.008	-	12.0 cm
	Vessel		Round	16,233 × g
	4 to 8 mL		Ø 13,5 mm	19,246 × g
	1/6	5820 725.001	119 mm	12.0 cm

28

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

Vessel	Vessel	Adapters	Adapter bottom	Max. g-force at 11,000 rpm
			shape	(5804/5804 R/5810)
	Capacity	Order no.	Tube diameter	Max. g-force at 12,100 rpm
		(international)		(5810 R)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with rotor lid
	Vessel		Round	16,233 × g
	5.5 mL – 10 mL		Ø 16 mm	19,246 × g
	1/6		-	12.0 cm
		5820 728.000
	Vessel		Round	16,233 × g
	7.5 to 12 mL		Ø 16.4 mm	19,246 × g
	1/6	5820 727.004	119 mm	12.0 cm
	Vessel		Round	16,233 × g
	9 mL		Ø 16.4 mm	19,246 × g
	1/6	5820 729.007	112 mm	12.0 cm
2.4.7 Rotor F-34-6-38
		Max. g-force:	15,557 × g (5810 R: 18,514 × g)
	Rotor F-34-6-38	Max. speed:	11,000 rpm (5810 R: 12,000 rpm)
	Fixed-angle rotor for	Max. load (adapter,	6 × 125 g
	6 x 85 mL tubes
		tube and contents):

EN

manual Operating

Vessel	Vessel	Adapters	Adapter bottom	Max. g-force at 11,000 rpm
			shape	(5804/5804 R/5810)
	Capacity	Order no.	Tube diameter	Max. g-force at 12,100 rpm
		(international)		(5810 R)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with rotor lid
	Vessel		Round	15,300 × g
	1.5/2 mL		Ø 11 mm	18,200 × g
	4/24	5804 770.005	43 mm	11.3 cm
	Vessel		conical	14,150 × g
				16,842 × g
	5 mL	5804 777.000	Ø 17 mm	10,45 cm
	1/6		-
	Blood collection tube		Round	14,339 × g
	2 mL to 5 mL		Ø 13 mm	17,065 × g
	3/18	5804 738.004	80 mm	10.6 cm

29

Centrifuge 5804/5804 R/5810/5810 R — Operating manual

EN

Operating manual

Vessel	Vessel	Adapters	Adapter bottom	Max. g-force at 11,000 rpm
			shape	(5804/5804 R/5810)
	Capacity	Order no.	Tube diameter	Max. g-force at 12,100 rpm
		(international)		(5810 R)
	Vessels per		Max. tube length	Centrifugation radius
	adapter/rotor		with rotor lid
	Blood collection tube		Round	15,442 × g
	4 mL to 7 mL		Ø 13 mm	18,353 × g
	3/18	5804 739.000	107 mm	11.4 cm
	Vessel		Round	15,150 × g
	7 to 15 mL		Ø 16 mm	18,000 × g
	2/12	5804 771.001	112 mm	11.2 cm
	Conical tube		conical	14,450 × g
	15 mL		Ø 17.5 mm	17,200 × g
	1/6	5804 776.003	123 mm	10.7 cm
	Vessel		Round	14,750 × g
	15 to 18 mL		Ø 18 mm	17,550 × g
	1/6	5804 772.008	123 mm	10.9 cm
	Vessel		Round	14,900 × g
	20 mL to 30 mL		Ø 26 mm	17,700 × g
	1/6	5804 773.004	123 mm	11.0 cm
	Vessel		Round	15,157 × g
	50 mL		Ø 29 mm	18,014 × g
	1/6	5804 774.000	123 mm	11.2 cm
	Conical tube		conical	14,600 × g
	50 mL		Ø 29.5 mm	17,400 × g
	1/6	5804 775.007	121 mm	10.8 cm
	Vessel	-	-	15,550 × g
	85 mL		Ø 38 mm	18,500 × g
	-/6		121 mm	11.5 cm

30