Eppendorf Concentrator plus User Manual

Concentrator plus/Vacufuge® plus

Operating manual

Copyright© 2011 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner.

Trademarks

eppendorf, CombiSlide, and Vacufuge are registered trademarks of Eppendorf AG, Hamburg, Germany. Falcon and Microtainer are a registered trademarks of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or ® in this manual.

Operating manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211

5305 900.038-02/122011

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Fig. A: Front and rear view of the complete system with gel dryer connection

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	11	7	10 9 8 15		14
Fig. B: Front and rear view of the basic device
1	Pump outlet (complete system)			2	Lid made from PMMA
3	Sealing ring			4	Grip recess
5	Emergency release			6	Pump housing (complete system)
7	Name plate			8	Mains connection
9	Fuse holder			10	Mains power switch
11	Operating controls and device display			12	Emission condenser (complete system)
13	Gel dryer connection (complete system)			14	Pump connection (basic device)
15	Mains connection for external vacuum pump
	(basic device)

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Fig. C: Operating controls and device display for Concentrator plus and Vacufuge plus

		emission
		condenser
filter/		cold
hood		trap
		chemical
		trap

Fig. D: Connection options for the complete system

	cold						emission
	trap		pump			condenser				filter/
										hood
							cold
							trap
	solenoid		cold
	valve		trap				external
							vacuum
	cold		solenoid				system
	trap		valve

Fig. E: Connection options for the basic device

Concentrator plus/Vacufuge® plus — Operating manual

Table of contents

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .			7
1.1	Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		7
1.2	Warning signs and hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		7
	1.2.1	Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	7
	1.2.2	Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	7
1.3	Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		7
1.4	Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.3 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.3.1 Complete system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.3.2 Basic device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.2 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.4 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 2.4.1 Special notes on the individual rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3 Safety	. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	14
3.1	Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	14
3.2	User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	14
3.3	Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	14
	3.3.1 Declaration concerning the ATEX directive (94/9/EC). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	14
	3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	14
3.4	Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	15
3.5	Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	15
	3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	15
	3.5.2 Incorrect handling of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	16
	3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	16
	3.5.4 Extreme strain on the sample tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	17
	3.5.5 Vacuum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	18
3.6	Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	18

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.2 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.2.1 Open the packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.2.2 Lift the device out of the box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 4.2.3 Complete system: removing the transport protection screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 4.2.4 Place the device upright . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 4.2.5 Check the delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.3 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 4.3.1 General Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 4.3.2 Complete system: connecting the emission condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 4.3.3 Complete system: connecting the gel dryer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 4.3.4 Basic device: connecting the vacuum pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 5.2 Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 5.3 Preparation for concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.3.1 Switching on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.3.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.3.3 Warm-up phase. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.3.4 Loading a fixed-angle rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 5.3.5 Loading a swing-bucket rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 5.3.6 Closing the lid of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

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manual Operating

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Operating manual

Concentrator plus/Vacufuge® plus — Operating manual

5.4	Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		30
	5.4.1 Concentration with time setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		30
	5.4.2 Concentration with continuous run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		31
	5.4.3 During or after concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		31
	5.4.4	Completing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	31
	5.4.5 Removing the rotor and switching off the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		32
	5.4.6 Emptying the emission condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		32
5.5	Notes on using rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		32
	5.5.1 RotorA-2-VC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		32
5.6	Special function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		33
	5.6.1 Operation as a desiccator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		33
	5.6.2 Operation with gel dryer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		33
	5.6.3 Operation as a centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		33
6 Maintenance .		. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	34
6.1	Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		34
	6.1.1	Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	34
	6.1.2	Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	34
6.2	Prepare cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		34
6.3	Cleaning / Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		35
	6.3.1 Cleaning / Disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		35
	6.3.2 Cleaning / Disinfecting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		36
6.4	Glass breakage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		36
6.5	Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		36
6.6	Decontamination before shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		36

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 7.3 Opening the device in case of a power failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 8.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 8.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 9.3 Weight / dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 9.4 Diaphragm vacuum pump for the complete system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 9.5 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

10 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

10.1 Complete system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 10.2 Basic device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 10.3 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 10.4 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 10.4.1 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 10.4.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 10.4.3 Other accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

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Concentrator plus/Vacufuge® plus — Operating manual

1User instructions

1.1Using this manual

Read this operating manual completely before using the device for the first time.

Consider this operating manual as part of the product and keep it in a place where it can be easily accessed.

Include this operating manual with the device when passing the device on to third parties.

In case of loss of the operating manual, please request a replacement. The latest version can be found on our website at www.eppendorf.com.

1.2Warning signs and hazard icons

1.2.1Hazard symbols

Biohazard	Explosion
Electric shock	Hot surface
Crushing	Toxic substances
Hazard point	Material damage

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1.2.2Degrees of danger

The degree of danger is a part of a safety note and distinguishes the possible results of non-observance from each other.

DANGER	Will lead to severe injuries or death.
WARNING	May lead to severe injuries or death.
CAUTION	May lead to light to moderate injuries.
NOTICE	May lead to material damage.

1.3Symbols used

Depiction Meaning

You are requested to perform an action.

1. Perform these actions in the sequence described.

2.

•List.

References useful information.

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Concentrator plus/Vacufuge® plus — Operating manual

1.4Abbreviations used

ETFE Ethylene/Tetrafluorethylene copolymer FFKM Perfluoroelastomer

MTP Micro test plate

NN Mean sea level (MSL) PCR Polymerase Chain Reaction

PMMA Polymethyl methacrylate PTFE Polytetrafluorethylene

rcf Relative centrifugal force (g value, RZB) rpm Revolutions per minute (min-1)

RCF relative centrifugal force (g value) UV Ultraviolet radiation

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Concentrator plus/Vacufuge® plus — Operating manual

2Product description

2.1Main illustration

The fold-out page depicts front and rear views of the complete system (see Fig. A) and the basic device (see Fig. B).

1	Pump outlet (complete system)	2	Lid made from PMMA
	Outlet of the integrated diaphragm
	vacuum pump, e.g., for tubing connection
	to the emission condenser.
3	Sealing ring	4	Grip recess
5	Emergency release	6	Pump housing (complete system)
7	Name plate	8	Mains connection
9	Fuse holder	10	Mains power switch
11	Operating controls and device display	12	Emission condenser (complete
			system)
13	Pump connection (basic device)	14	Mains connection for external vacuum
	For tubing connection to external vacuum		pump (basic device)
	pump.

2.2Features

Concentrator plus and Vacufuge plus are designed for the evaporation of liquid or wet samples in micro test tubes, round-bottom tubes, Falcon tubes, flat-bottom tubes and different plates.

You can insert the following tubes and plates:

•1.5 and 2.0 mL micro test tubes in a rotor with 70 places.

•0.5 mL micro test tubes in a rotor with 72 places.

•15 mL Falcon tubes in a rotor with 8 places.

•50 mL Falcon tubes in a rotor with 6 places.

•MTP and PCR plates in a swing-bucket rotor.

A complete list can be found in the following chapter (see Rotors on p. 11)

The device is available as a complete system with integrated diaphragm vacuum pump or as a basic device without vacuum pump. The basic device can be connected to an external vacuum system.

Key, practical functions of Concentrator plus or Vacufuge plus have been optimized:

•3 temperature levels can be set (30, 45, 60 °C). Alternatively, evacuation is carried out without thermoregulation.

•The evaporation of liquids can be carried out optimized in 3 functions. In addition to pure evacuation, aqueous and/or alcoholic solutions can be concentrated extra fast with 2 special functions.

•You can also operate the device as a pure desiccator.

•You can connect a solvent trap to the device behind the pump.

Due to the space-saving design, the device can be situated directly on the workstation. With its clearly laid-out control panel with only a few, clearly marked elements and a display that is easy to read, the device is a pleasant and reliable companion in your daily work.

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Concentrator plus/Vacufuge® plus — Operating manual

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2.3Delivery package

Operating manual

The delivery package includes one of the following device/rotor combinations and the accessories listed below.

2.3.1Complete system

Quantity	Order No.	Order No.	Description
	(International)	(North America)
			Concentrator plus/Vacufuge plus complete system
1	5305 000.304	-	with rotor F-45-48-11
			230 V/50 - 60 Hz, with integrated diaphragm vacuum pump
or	5305 000.312	5305000312	120 V/50 - 60 Hz, with integrated diaphragm vacuum pump
or	5305 000.347	-	100 V/50 - 60 Hz, with integrated diaphragm vacuum pump
			Concentrator plus/Vacufuge plus complete system
or	5305 000.509	-	without rotor
			230	V/50 - 60 Hz, with integrated diaphragm vacuum pump
or	5305 000.517	5305000517	120	V/50 - 60 Hz, with integrated diaphragm vacuum pump
or	5305 000.541	-	100	V/50 - 60 Hz, with integrated diaphragm vacuum pump
			Concentrator plus/Vacufuge plus complete system
or	5305 000.703	-	with connection, e.g., for a gel dryer, without rotor
			230	V/50 - 60 Hz, with integrated diaphragm vacuum pump
or	5305 000.711	5305000711	120	V/50 - 60 Hz, with integrated diaphragm vacuum pump
or	5305 000.746	-	100	V/50 - 60 Hz, with integrated diaphragm vacuum pump
			Fuse
1	5301 850.249	022654403	2 x 4.0 A T (230 V)
or	5417 341.007	022375831	2 x 6.3 A T UL (120 V / 100 V)
1	-	-	Power cable
1	5305 900.038	-	Operating Manual Concentrator plus/Vacufuge plus
			Emission condenser
1	5301 330.008	022830309	without hose
			Hose for emission condenser
1	5301 337.002	022830295	length: 0.7 m
			Tube connection
1	5301 110.032	5301110032	Plastic
2.3.2 Basic device
Quantity	Order No.	Order No.	Description
	(International)	(North America)
			Concentrator plus/Vacufuge plus basic device
			with rotor F-45-48-11
1	5305 000.100	-	230	V/50 - 60 Hz
or	5305 000.118	5305000118	120	V/50 - 60 Hz
or	5305 000.142	-	100	V/50 - 60 Hz
			Fuse
1	5301 850.249	022654403	2 x 4.0 A T (230 V)
or	5417 341.007	022375831	2 x 6.3 A T UL (120 V / 100 V)
1	-	-	Power cable
1	5305 900.038	-	Operating Manual Concentrator plus/Vacufuge plus

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Concentrator plus/Vacufuge® plus — Operating manual

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2.4Rotors

You can operate the device with the following rotors. Before use of sample tubes, please note the manufacturer's specifications with regard to centrifugation resistance (max. rcf).

Rotor	Tube			Max. capacity	Tube dimensions	Max. g-force (rcf)/	Max. payload
					(mm) Ø × L	speed (rpm)	per rotor bore(1)
					and/or L × W × H
F-45-72-8				72 micro test tubes of	8 × 31	inner ring:	10 g
				0.5 mL each.		202 x g,
						outer ring:
						224 x g/
						1,400 rpm
F-45-70-11				70 micro test tubes of	11 × 41/	inner ring:	15 g
				1.5/2.0 mL each.	11 × 47	153 x g,
						middle ring:
						202 x g,
						outer ring:
						248 x g/
						1,400 rpm
F-45-48-11				48 micro test tubes	11 × 41/	inner ring:	15 g
				of 1.5/2.0 mL each.	11 × 47	217 x g,
						outer ring:
						239 x g/
						1,400 rpm
F-45-24-12				24 round-bottom tubes	12 × 67-100	234 x g/	35 g
				of 6.0/8.0 mL each.		1,400 rpm
F-50-8-16				8 round-bottom tubes	16 × 105-120	230 x g/	70 g
				of 15.0/20.0 mL each.		1,400 rpm
F-50-8-18				8 round-bottom tubes	18 × 105-128	230 x g/	70 g
				of 15.0/20.0 mL each.		1,400 rpm
F-35-6-30				6 Falcon tubes	17 × 116-123	232 x g/	25 g
				of 15 mL each.		1,400 rpm
F-45-8-17				8 Falcon tubes	17 × 118-123	239 x g/	70 g
				of 15 mL each.		1,400 rpm
F-35-6-30				6 Falcon tubes	29.5 × 116-123	232 x g/	75 g
				of 50 mL each.		1,400 rpm

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Concentrator plus/Vacufuge® plus — Operating manual

Rotor	Tube	Max. capacity		Tube dimensions		Max. g-force (rcf)/	Max. payload
				(mm) Ø × L		speed (rpm)	per rotor bore(1)
				and/or L × W × H
F-40-36-12		36 flat-bottom tubes of		12	× 32	215 x g/	15 g
		1.5 mL each.				1,400 rpm
F-45-36-15		36 flat-bottom tubes of		15	× 45-48	217 x g/	20 g
		3.0/5.0 mL each.				1,400 rpm
F-45-16-20		16 flat-bottom tubes		20	× 42-55	217 x g/	45 g
		of 6.5/10.0 mL each.				1,400 rpm
F-40-18-19		18 flat-bottom tubes of		19	× 66	228 x g/	35 g
		10.0 mL each.				1,400 rpm
F-45-12-31		12 flat-bottom tubes of		31	× 55	226 x g/	55 g
		20.0 mL each.				1,400 rpm
F-35-8-24		8 flat-bottom tubes of		24	× 86-90	232 x g/	90 g
		25.0 mL each.				1,400 rpm
A-2-VC		Two buckets to hold		128 × 86 × 27		131 x g/	115 g per bucket
		•	0.2 mL PCR tubes			1,400 rpm
		•	PCR strips of 8
			and of 5
		•	PCR plates
		•	Microplates
		•	Cell culture plates
		•	Deepwell plates
			(max. height =
			27 mm)
		•	Slides (with	26	× 75
			CombiSlide
			adapter)

(1) Maximum payload per rotor bore for adapter + tube + content.

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Concentrator plus/Vacufuge® plus — Operating manual

2.4.1Special notes on the individual rotors

Rotor F-45-72-8 and rotor F-45-48-11

•You can place two of these rotors on top of each other in any arrangement by using a spacer (order no. int.: 5301 316.005 / North America: 022822101) and centrifuge them at the same time.

•For this combination, lower upper limits apply to the maximum payload: bottom rotor: 2/3 of the max. payload, top rotor 1/3 of the max. payload.

•Rotor F-45-72-8 and F-45-48-11: Included with the accessories are rotor feet which can be screwed into the corresponding bores on the rotor. Their purpose is to prevent the samples from being pushed out of the bores when the rotor is put down on the work bench. The rotors can be centrifuged with the rotor feet. The screws must be checked monthly and re-tightened, if required.

Rotor F-45-24-12

•Tube length 75 mm: max. capacity 24 tubes.

•Tube length > 75 mm: max. capacity 12 tubes.

Rotor A-2-VC

•Tubes, PCR strips and plates without frame can only be used with a suitable work plate (see Adapters on p. 44).

•Max. loading height: 27 mm.

Rotor F-35-6-30

•The recommended maximum filling volume for 50 mL Falcon tubes is 44 mL. If the tubes are filled up to the top, the rotational movement may cause the samples to overflow.

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Concentrator plus/Vacufuge® plus — Operating manual

3Safety

3.1Intended use

Concentrator plus and Vacufuge plus are intended for sample preparation. The device may only be operated by trained specialist staff.

Concentrator plus and Vacufuge plus are intended for interior use only and mainly intended for the concentration of aqueous solutions of nucleic acids and proteins in approved sample tubes.

Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may

impair the safety, function and precision of the device. Eppendorf cannot be held liable or accept CAUTION! any liability for damage resulting from the use of incorrect or non-recommended accessories and

spare parts or from the improper use of such equipment.

Only use accessories and original spare parts recommended by Eppendorf.

3.2User profile

This device may only be operated by correspondingly trained specialist staff. This staff must have carefully read the operating manual and be familiar with the function of the device.

3.3Application limits

Continuous delivery can affect safe operation of the device.

		When delivering liquids continuously, membranes and valves of the vacuum pump can be
	CAUTION!	damaged.
		Only use the device for applications of limited duration.

3.3.1Declaration concerning the ATEX directive (94/9/EC)

Danger of explosion!

Do not operate the device in areas where work is completed with explosive substances.

	DANGER!	Do not use this device to process any explosive or highly reactive substances.
		Do not use this device to process any substances which could create an explosive
		atmosphere.

Due to their current design and the environmental conditions on the inside of the device, the Concentrator plus / Vacufuge plus are not suitable for use in a potentially explosive atmosphere.

The device must therefore only be used in a safe environment, such as in the open environment of a ventilated laboratory or an extractor hood. The use of substances which may contribute to a potentially explosive atmosphere is not permitted. The final decision with regard to the risks connected with the use of such substances is the responsibility of the user.

3.3.2Maximum service life for accessories

The maximum service life for plastic adapters is 1 year from first commissioning. Do not use the adapter beyond this period!

For the rotors (see Rotors on p. 11) described here there is no limit for their service life, as long as the following conditions are met: proper use, recommended maintenance and undamaged condition.

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Concentrator plus/Vacufuge® plus — Operating manual

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3.4Information on product liability

In the following cases, the protection provided by the device may be impaired. The liability for the function of the device passes to the operator if:

•The device is not used in accordance with the operating manual.

•The device is used outside of the range of application described in the preceding chapters.

•The device is used with accessories or consumables (e.g., tubes and plates) which are not recommended by Eppendorf.

•The device is maintained or repaired by persons not authorized by Eppendorf.

•The owner has made unauthorized modifications to the device.

3.5Warnings for intended use

Before using the device, read the operating manual and observe the following general safety instructions.

3.5.1Personal injury or damage to the equipment

		Electric shock from damage to device or mains cable.
		Only switch on the device if the device and the mains cable are undamaged.
	WARNING!	Only use devices that have been properly installed or repaired.
		In case of danger, disconnect the device from the mains.
		Lethal voltages inside the device.
		Ensure that the housing is always closed and undamaged so that no parts inside the device
	WARNING!	can be contacted by accident.
		Do not remove the housing of the device.
		Do not allow any liquids to penetrate the inside of the housing.
		Do not allow the device to be opened by anyone except service personnel who have been
		specifically authorized by Eppendorf.

Risk from incorrect supply voltage.

		Only connect the device to power sources which correspond with the electrical requirements
	WARNING!	on the name plate.
		Only use sockets with a protective earth (PE) conductor and a suitable mains cable.

Damage to health due to handling infectious liquids and pathogenic germs.

		Observe the national regulations for handling these substances, the biological security level
	WARNING!	of your laboratory, the safety data sheets and the manufacturer's application notes.
		Wear personal protective equipment (PPE).

Follow the instructions regarding hygiene, cleaning and decontamination.

For complete instructions regarding the handling of germs or biological material of risk group II or higher, please refer to the "Laboratory Biosafety Manual" (source: World Health Organization, current edition of the Laboratory Biosafety Manual).

Burns due to hot sample tubes and device parts.

		If the heater is at full power, the wall of the rotor chamber, the rotor and the sample tubes will be
	WARNING!	heated up to over 60°C.
		Do not touch the wall of the rotor chamber.

Use suitable protective clothing or auxiliary aids to remove the sample tubes.

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