Eppendorf 5427 R User Manual [en, es]

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Centrifuge 5427 R

Operating manual

Manual de instrucciones

Copyright © 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner.

Trademarks

Eppendorf and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany. Microtainer is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or ® in this manual.

Operating manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Manual de instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Declarations and Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

5409 900.021-02/072013

Table of contents

Centrifuge 5427 R 3

English (EN)

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2.4 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.4.1 Rotor FA-45-12-17. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.4.2 Rotor FA-45-24-11. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.4.3 Rotor FA-45-24-11-Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 2.4.4 Rotors FA-45-30-11 and F-45-30-11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 2.4.5 Rotors FA-45-48-11 and F-45-48-11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2.4.6 Rotor F-45-48-5-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 2.4.7 Rotor S-24-11-AT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 3.3 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 3.3.1 Declaration concerning the ATEX directive (94/9/EC) . . . . . . . . . . . . . . . . . . . . . . . . . 15 3.3.2 Maximum service life for accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 3.5.2 Incorrect handling of the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 3.5.4 Extreme strain on the centrifuging tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

3.6 Safety instructions located on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 4.2 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 4.3 Installing the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 5.2 Menu navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 5.3 Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 5.4 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.4.1 Switching on the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.4.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.4.3 Automatic rotor detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.4.4 Replacing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 5.4.5 Loading the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 5.4.6 Closing the rotor lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 5.4.7 Closing the centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Table of contents

4Centrifuge 5427 R English (EN)

5.5 Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 5.5.1 Set the temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 5.5.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 5.5.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 5.5.4 Start FastTemp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 5.5.5 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 5.5.6 Endless operation of continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

5.6 Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 5.6.1 Centrifuging with preset time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 5.6.2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 5.6.3 Short spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 5.6.4 Setting the centrifugation radius . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 5.6.5 After centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.7 Standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

6.1 Prepare cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 6.2 Cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 6.2.1 Cleaning and disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 6.2.2 Cleaning and disinfecting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

6.3 Additional service instructions for Centrifuge 5427 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 6.4 Glass breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 6.5 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 6.6 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 7.3 Emergency release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

8.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 8.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 8.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 9.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 9.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

9.5Acceleration times and deceleration times for the Centrifuge 5427 R (according to DIN 58 970)

49

10 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

10.1 Centrifuge 5427 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 10.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 10.2.1 Rotors and rotor lids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 10.2.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 10.2.3 Other accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 10.3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Operating instructions

Centrifuge 5427 R 5

English (EN)

1Operating instructions

1.1Using this manual

Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable.

This operating manual is part of the product. Thus, it must always be easily accessible.

Enclose this operating manual when transferring the device to third parties.

If this manual is lost, please request another one. You will find the current version on our webpage www.eppendorf.com.

1.2Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1Danger symbols

Biohazard	Explosion
Electric shock	Crushing
Hazard point	Material damage

1.2.2Danger levels

DANGER	Will lead to severe injuries or death.
WARNING	Can lead to severe injuries or death.
CAUTION	Can lead to light to moderate injuries.
ATTENTION	May lead to material damage./Paragraph

1.3Symbols used

Depiction			Meaning
1.			Actions in the specified order
2.
			Actions without a specified order
•			List:
Text			Display or software texts
			Additional information

Operating instructions

6Centrifuge 5427 R English (EN)

1.4Abbreviations used

NN

Mean sea level (MSL)

PCR

Polymerase chain reaction

RZB/rcf

Relative centrifugal force – g-force in m/s2

rpm

Revolutions per minute – in rpm

UV

Ultraviolet radiation

Product description

Centrifuge 5427 R 7

English (EN)

2Product description

2.1Main illustration

				USB
	6			7
1
2
		open
3	menu	short
	enter
		start
		stop
4	rpm
	rcf
	fast
	temp
5
		8	9 10	11

Fig. 2-1: Front and rear view of the Centrifuge 5427 R

1	Centrifuge lid	7	USB port
2	Monitoring glass		Only for Technical Service: interfaces for error
			analyses and software updates
	Visual control for rotor stop or speed control
	option using stroboscope	8	Mains/power switch
3	Display		Switch for switching the device on (I) and off (0)
	Display of centrifugation parameters and device	9	Fuse holder
	settings (see Fig. 5-2 on p. 24)	10	Power connection socket
4	Control panel		Connection for supplied power cable
	Keys and dials for operating centrifuge (see	11	Name plate
	Fig. 5-1 on p. 23)

5 Condensation water tray (only)

6Emergency release

(see p. 44)

Product description

8Centrifuge 5427 R English (EN)

2.2 Delivery package

Quant	Order no.	Order no.	Description
ity	(International)	(North America)
			Centrifuge 5427 R
1	-	-	See chapter Ordering Information for corresponding device
			version, equipment and order number
			Fuse
1	5301 850.249	022654403	4.0 A (230 V), 2 pieces
or	5427 850.341	022654381	8.0 AT UL (120 V/100 V), 2 pieces
or	5811 352.006	022664107	10.0 AT UL (100 V), 2 pieces
			Rotor key
1	5416 301.001	022634305	Standard
1	-	-	Mains power cable
1	5409 850.083		Tray for condensation water
			Operating Manual Centrifuge 5427 R
1	5409 900.021	5409900021	Languages: EN, DE, FR, ES, IT, PT
1	5409 900.030	5409900030	Languages: DA, FI, EL, NL, SV (only 230 V devices)

2.3Features

The high-performance Centrifuge 5427 R has a capacity of 48 × 2 mL and maximally reaches 25 001 × g resp. maximally 16 220 rpm. You can select from 9 different rotors to centrifuge the following tubes for your various applications:

•Tubes (0.2 mL to 5.0 mL)

•PCR strips

•Microtainers (0.6 ml)

•Spin columns (1.5 mL, 2.0 mL)

The Centrifuge 5427 R has a temperature function for centrifugation at temperatures of-11 °C to 40 °C. The FastTemp function is used to start a temperature control run without samples in order to quickly bring the rotor chamber to the set temperature.

Product description

Centrifuge 5427 R 9

English (EN)

2.4Rotors

Eppendorf centrifuges may only be operated with rotors that are intended for use with the corresponding centrifuge.

Only use rotors which are marked with the name of the centrifuge (e.g., 5427 R).

2.4.1Rotor FA-45-12-17

	Rotor FA-45-12-17	Max. g-force:		20 598 × g
		Max. speed:		14 000 rpm
	Aerosol-tight fixed-angle rotor
	for 12 reaction tubes	Max. load (adapter, tube		12 × 9.5 g
		and contents):
Vessels	Vessel	Adapters	Adapter base	Max. g-force:
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per			Centrifugation radius
	adapter/rotor
	Vessel	–	–	20 598 × g
	5 mL		Ø 17 mm	14 000 rpm
	-/12			9.4 cm

Vessel	open	17 530 × g
1.5 mL/2.0 mL	Ø 11 mm	14 000 rpm
1/12	5820 768.002	8.0 cm
HPLC vessel	flat	16 215 × g
	Ø 11 mm	14 000 rpm
1/12	5820 770.007	7.4 cm
Cryo tube	flat	18 188 × g
1.0 mL – 2.0 mL	Ø 13 mm	14 000 rpm
1/12	5820 769.009	8.3 cm

Product description

10Centrifuge 5427 R English (EN)

2.4.2 Rotor FA-45-24-11

	Rotor FA-45-24-11	Max. g-force:		25 001 × g
		Max. speed:		16 220 rpm
	Aerosol-tight fixed-angle rotor
	for 24 tubes	Max. load (adapter, tube		24 × 3.75 g
		and contents):
Vessels	Vessel	Adapters	Adapter base	Max. g-force:
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per			Centrifugation radius
	adapter/rotor
	Vessel	–	–	25 001 × g
	1.5 mL/2.0 mL		Ø 11 mm	16 220 rpm
	-/24			8.5 cm
	PCR tube		conical	18 825 × g
	0.2 mL		Ø 6 mm	16 220 rpm
	1/24	5425 715.005		6.4 cm
	Vessel		conical	25 001 × g
	0.4 mL		Ø 6 mm	16 220 rpm
	1/24			8.5 cm
		5425 717.008
	Vessel		open	21 766 × g
	0.5 mL		Ø 8 mm	16 220 rpm
	1/24			7.4 cm
		5425 716.001
	Microtainers		open	25 001 × g
	0.6 mL		Ø 8 mm	16 220 rpm
	1/24			8.5 cm
		5425 716.001

Product description

Centrifuge 5427 R 11

English (EN)

2.4.3Rotor FA-45-24-11-Kit

	Rotor FA-45-24-11-Kit	Max. g-force:		19 090 × g
		Max. speed:		13 200 rpm
	Aerosol-tight fixed-angle rotor
	for 24 tubes	Max. load (adapter, tube		24 × 3.75 g
		and contents):
Vessels	Vessel	Adapters	Adapter base	Max. g-force:
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per			Centrifugation radius
	adapter/rotor
	Vessel	–	–	19 090 × g
	1.5 mL/2.0 mL		Ø 11 mm	13 200 rpm
	-/24			9.8 cm
	PCR tube		conical	15 000 × g
	0.2 mL		Ø 6 mm	13 200 rpm
	1/24	5425 715.005		7.7 cm
	Vessel		conical	19 090 × g
	0.4 mL		Ø 6 mm	13 200 rpm
	1/24			9.8 cm
		5425 717.008
	Vessel		open	16 948 × g
	0.5 mL		Ø 8 mm	13 200 rpm
	1/24			8.7 cm
		5425 716.001
	Microtainers		open	19 090 × g
	0.6 mL		Ø 8 mm	13 200 rpm
	1/24			9.8 cm
		5425 716.001

Product description

12Centrifuge 5427 R English (EN)

2.4.4 Rotors FA-45-30-11 and F-45-30-11

	Rotor FA-45-30-11		Max. g-force:		20 817 × g
	Aerosol-tight fixed-angle rotor		Max. speed:		14 000 rpm
	for 30 tubes
			Max. load (adapter, tube		30 × 3.75 g
	Rotor F-45-30-11
			and contents):
	Fixed-angle rotor for 30 tubes
Vessels	Vessel	Adapters		Adapter base	Max. g-force:
	Capacity	Order no.		Tube diameter	Max. speed
		(international)
	Vessels per				Centrifugation radius
	adapter/rotor
	Vessel	–		–	20 817 × g
	1.5 mL/2.0 mL			Ø 11 mm	14 000 rpm
	-/30				9.5 cm
	PCR tube			conical	16 215 × g
	0.2 mL			Ø 6 mm	14 000 rpm
	1/30	5425 715.005			7.4 cm
	Vessel			conical	20 817 × g
	0.4 mL			Ø 6 mm	14 000 rpm
	1/30				9.5 cm
		5425 717.008
	Vessel			open	18 407 × g
	0.5 mL			Ø 8 mm	14 000 rpm
	1/30				8.4 cm
		5425 716.001
	Microtainers			open	20 817 × g
	0.6 mL			Ø 8 mm	14 000 rpm
	1/30				9.5 cm
		5425 716.001

Product description

Centrifuge 5427 R 13

English (EN)

2.4.5Rotors FA-45-48-11 and F-45-48-11

	FA-45-48-11 rotor		Max. g-force:		18 213	× g (outer ring)
					16 049	× g (inner ring)
	Aerosol-tight fixed-angle rotor
	for 48 tubes		Max. speed:		12 700 rpm
	Rotor F-45-48-11		Max. load (adapter, tube		48 × 3.75 g
	Fixed-angle rotor for 48 tubes
			and contents):
Vessels	Vessel	Adapters		Adapter base	Max. g-force:
					Outer ring
					Inner ring
	Capacity	Order no.		Tube diameter	Max. speed
		(international)
	Vessels per				Centrifugation radius
	adapter/rotor				Outer ring
					Inner ring
	Vessel	–		–	18 213	× g
					16 049	× g
	1.5 mL/2.0 mL			Ø 11 mm	12 700 rpm
	-/48				10.1 cm
					8.9 cm
	PCR tube			conical	14 426	× g
					12 262	× g
	0.2 mL	5425 715.005		Ø 6 mm	12 700 rpm
	1/48				8 cm
					6.8 cm
	Vessel			conical	18 213	× g
					16 049	× g
	0.4 mL			Ø 6 mm	12 700 rpm
	1/48	5425 717.008			10.1 cm
					8.9 cm
	Vessel			open	16 229	× g
					14 065	× g
	0.5 mL			Ø 8 mm	12 700 rpm
	1/48	5425 716.001			9 cm
					7.8 cm
	Microtainers			open	18 213	× g
					16 049	× g
	0.6 mL			Ø 8 mm	12 700 rpm
	1/48	5425 716.001			10.1 cm
					8.9 cm

Product description

14Centrifuge 5427 R English (EN)

2.4.6 Rotor F-45-48-5-PCR

Rotor F-45-48-5-PCR

Fixed-angle rotor for 48 PCR tubes

Max. g-force:	11	710 × g
Max. speed:	10	500 rpm
Max. load (tube and	48	× 0.43 g
contents):

Tubes	Tube	Adapter	Adapter base	Max. g-force:
	Capacity		Tube diameter	Max. speed
	Tubes per adapter/			Centrifugation radius
	rotor
		–	conical	11 710 × g
	0.2 mL		Ø 6 mm	10 500 rpm
	–/48			9.5 cm
	PCR strips	–	conical	11 710 × g
	0.2 mL		Ø 6 mm	10 500 rpm
	–/6 × 8			9.5 cm

2.4.7Rotor S-24-11-AT

This rotor is only intended for use with 1.5 mL/2.0 mL tubes.

The following tubes must not be used in this rotor:

•Adapters for 0.2 mL, 0.4 mL, 0.5 mL and 0.6 mL tubes and the corresponding tubes.

•Spin columns

Rotor S-24-11-AT Bucket for tubes:	Max. g-force:	16 049 × g
4 × 1.5 mL/2.0 mL
	Max. speed:	12 700 rpm
	Max. load per bucket (tube	4 × 3.75 g
	and contents):

Vessels	Vessel	Adapters	Adapter base	Max. g-force:
	Capacity	Order no.	Tube diameter	Max. speed
		(international)
	Vessels per			Centrifugation radius
	adapter/rotor
	Vessel	–	–	16 049 × g
	1.5 mL/2.0 mL		Ø 11 mm	12 700 rpm
	-/24			8.9 cm

Safety

Centrifuge 5427 R 15

English (EN)

3Safety

3.1Intended use

The Centrifuge 5427 R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes.

3.2User profile

This device may only be operated by trained specialist staff. They must have carefully read the operating manual and be familiar with the function of the device.

3.3Application limits

3.3.1Declaration concerning the ATEX directive (94/9/EC)

DANGER! Risk of explosion.

Do not operate the device in areas where work is completed with explosive substances.

Do not use this device to process any explosive or highly reactive substances.

Do not use this device to process any substances which may generate an explosive atmosphere.

Due to its design and the environmental conditions inside the device, the Centrifuge 5427 R is not suitable for use in a potentially explosive atmosphere.

The device only must be used in a safe environment, such as the open environment of a ventilated laboratory or fume hood. The use of substances which could create a potentially explosive atmosphere is not permitted. The final decision on the risks associated with the use of these types of substances is the responsibility of the user.

3.3.2Maximum service life for accessories

WARNING! Risk of injury from chemically or mechanically damaged accessories.

Even minor scratches and cracks can lead to serious internal material damage.

Protect all accessory parts from mechanical damage.

Inspect the accessories for damage before each use. Replace any damaged accessories.

Do not use rotors, rotor lids or buckets with signs of corrosion or mechanical damage (e.g., deformations).

Do not use any accessories whose maximum service life has been exceeded.

CAUTION! Risk of injury due to chemically damaged rotor lids or caps.

Transparent rotor lids or caps made from PC, PP or PEI may lose their strength under the impact of organic solvents (e.g., phenol, chloroform).

If rotor lids or caps have come into contact with organic solvents, they should be cleaned immediately.

Regularly check the rotor lids and caps for damages and cracks.

Immediately replace rotor lids or caps that have cracks or milky stains.

Safety

16Centrifuge 5427 R English (EN)

Beginning with the first commissioning, the following rotors including the corresponding buckets, carriers and rotor lids have a maximum service life of 7 years or of the number of cycles specified in the table (depending on what occurs first).

Rotor/accessories	Maximum service from commissioning onward
S-24-11-AT (5427 R/5430/5430 R)	75,000 cycles	7 years
FA-45-48-11	75,000 cycles	7 years
(5427 R/5430/5430 R/5804/5804 R/5810/5810 R)
FA-45-12-17 (Centrifuge 5427 R)	75,000 cycles	7 years
QuickLock rotor lid		3 years
Seals of the QuickLock rotor lid	50 autoclaving cycles	–
Rotor lid or caps made of polycarbonate ( PC),	50 autoclaving cycles	3 years
polypropylene (PP) or polyetherimide (PEI)
Adapter	–	1 year

For all other rotors and rotor lids of this centrifuge there is no service life limit as long as the following requirements are met:

•Proper use,

•Recommended maintenance

•Undamaged condition

The date of manufacture is stamped on the rotors in the format 03/10 (= March 2010) or on the inside of the plastic rotor lids in the form of a clock .

To ensure aerosol tightness, the following applies:

•Replace aerosol-tight rotor lids and caps after 50 autoclaving cycles.

•Replace the seal of QuickLock rotor lids after 50 autoclaving cycles.

3.4Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

•The device is not used in accordance with the operating manual.

•The device is used outside of its intended use.

•The device is used with accessories or consumables which are not recommended by Eppendorf.

•The device is maintained or repaired by people not authorized by Eppendorf.

•The user makes unauthorized changes to the device.

Safety

Centrifuge 5427 R 17

English (EN)

3.5Warnings for intended use

Read the operating manual and observe the following general safety instructions before using the Centrifuge 5427 R.

3.5.1Personal injury or damage to the equipment

WARNING! Electric shock due to damage to device or mains cable.

Only switch on the device if the device and mains cable are undamaged.

Only use devices that have been properly installed or repaired.

In case of danger, disconnect the device from the mains supply by pulling the power plug from the device or the mains socket or, by using the isolating device intended for this purpose (e.g. emergency stop switch in the laboratory).

WARNING! Lethal voltages inside the device.

Ensure that the housing is always closed and undamaged so that no parts inside the device can be contacted by accident.

Do not remove the housing of the device.

Do not allow any liquids to penetrate the inside of the housing.

Do not allow the device to be opened by anyone except service personnel who have been specifically authorized by Eppendorf.

WARNING! Risk from incorrect supply voltage

Only connect the device to voltage sources which correspond to the electrical requirements on the name plate.

Only use sockets with a protective earth (PE) conductor and suitable power cable.

WARNING! Damage to health due to infectious liquids and pathogenic germs.

When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

Use aerosol tight sealing systems for the centrifugation of these substances.

When working with pathogenic germs belonging to a higher risk group, more than one aerosol-tight bioseal must be used.

Wear personal protective equipment.

For full instructions regarding the handling of germs or biological material of risk group II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual).

WARNING! Risk of injury when opening or closing the centrifuge lid.

There is a risk of crushing your fingers when opening or closing the centrifuge lid.

When opening or closing the centrifuge lid, do not reach between the lid and device or into the latching mechanism of the lid.

Always open the centrifuge lid completely to prevent it from falling.

Safety

18Centrifuge 5427 R English (EN)

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.

Only use accessories and original spare parts recommended by Eppendorf.

NOTICE! Damage to device due to spilled liquids.

1.Switch off the device.

2.Disconnect the device from the power supply.

3.Carefully clean the device and the accessories in accordance with the cleaning and disinfection instructions in the operating manual.

4.If a different cleaning and disinfecting method is to be used, contact Eppendorf AG to ensure that the intended method will not damage the device.

NOTICE! Damage to electronic components due to condensation.

Condensate can form in the device after it has been moved from a cool environment to a warmer environment.

After installing the device, wait for at least 4 h. Only then connect the device to the mains power supply.

3.5.2Incorrect handling of the centrifuge

NOTICE! Damage from knocking against or moving the device during operation.

If the rotor bangs against the rotor chamber wall, it will cause considerable damage to the device and rotor.

Do not move or knock against the device during operation.

3.5.3Incorrect handling of the rotors

WARNING! Risk of injury from improperly attached rotors and rotor lids.

Only centrifuge with rotor and rotor lid firmly tightened.

If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be properly secured. Immediately press the start/stop key to stop centrifuging.

CAUTION! Risk of injury due to asymmetric loading of a rotor.

Load rotors symmetrically with identical tubes.

Only load adapters with suitable tubes.

Always use the same type of tubes (weight, material/density and volume).

Check symmetric loading by balancing the adapters and tubes used with scales.

Safety

Centrifuge 5427 R 19

English (EN)

CAUTION! Risk of injury from overloaded rotor.

The Centrifuge 5427 R is designed for the centrifugation of material with a max. density of 1.2 g/mL at maximum speed and filling volume and/or load.

Do not exceed the maximum load of the rotor.

NOTICE! Damage to rotors from aggressive chemicals.

Rotors are high-quality components which withstand extreme stresses. This stability can be impaired by aggressive chemicals.

Avoid using aggressive chemicals, including strong and weak alkalis, strong acids, solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons, concentrated saline solutions and phenol.

Due to the manufacturing process, color variations may occur on rotors marked "coated". These color variations do not effect service life or resistance to chemicals.

NOTICE! If handled incorrectly, the rotor can fall over.

The rotor can fall over if the buckets are used as a handle.

Remove the buckets before inserting and/or removing a swing-bucket rotor.

Always use both hands to carry the rotor cross.

3.5.4Extreme strain on the centrifuging tubes

CAUTION! Risk of injury from overloaded tubes.

Note the loading limits specified by the tube manufacturer.

Only use tubes which are approved by the manufacturer for the required rcf.

NOTICE! Risk from damaged tubes.

Damaged tubes must not be used, as this could cause further damage to the device and the accessories and loss of the samples.

Before use, visually check all of the tubes for damage.

NOTICE! Risk from open tube lids.

Open tube lids can brake off during centrifugation and damage the rotor and the centrifuge.

Carefully seal all tube lids before centrifuging.

Exception: Note the information on the centrifugation of spin columns in the rotor FA-45-24-11-Kit (see Preparing for centrifugation on p. 27).

NOTICE! Hazard to plastic tubes from organic solvents.

The density of plastic tubes is reduced when organic solvents (e.g., phenol, chloroform) are used, i.e. the tubes could become damaged.

Observe the manufacturer's specifications for chemical resistance of the tubes.

Safety

20Centrifuge 5427 R English (EN)

3.5.5 Aerosol-tight centrifugation

WARNING! Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid combination.

Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this purpose are used. The designation of aerosol-tight fixed-angle rotors always starts with FA.The aerosol-tight rotors and rotor lids of this centrifuge are additionally marked with a red ring on the rotor and a red rotor lid screw.

Aerosol-tight swing-bucket rotors are marked with AT (aerosol-tight).

For aerosol-tight centrifugation, always simultaneously use rotors and rotor lids which are marked as aerosol-tight in the centrifuge intended for the corresponding purpose. The details specifying in which centrifuge you may use the aerosol-tight rotors and rotor lids can be found on the rotor and, beginning from production date of October 2003, on the upper side of the rotor lid.

Only use aerosol-tight rotor lids in combination with rotors which are marked on the rotor lid.

WARNING! Damage to health as a result of limited aerosol tightness and incorrect usage.

Autoclaving, mechanical stresses and contamination by chemicals or other aggressive solvents can impair the aerosol-tightness of the rotors and rotor lids.

Check the integrity of the seals of the aerosol-tight rotor lids or caps before each use.

Only use aerosol-tight rotor lids or caps if the seals are undamaged and clean.

Lightly grease the threads of the rotor lid screw with pivot grease after every proper autoclaving (121 °C, 20 min.) (int. order no. Int. 5810 350.050, North America 022634330).

Replace aerosol-tight rotor lids and caps after 50autoclaving cycles.

For QuickLock rotor lids, the seal must be replaced after 50 autoclaving cycles.

Never store aerosol-tight rotors or buckets closed.

3.6Safety instructions located on the device

Display	Meaning	Location
	Hazard point	Right side of the device
	CAUTION	Top of device, below the
	Always tighten the rotor using the supplied rotor	centrifuge lid
	key.
	CAUTION	Top of device, below the
	Close all tubes and use a rotor lid.	centrifuge lid

Installation

Centrifuge 5427 R 21

English (EN)

4Installation

4.1Selecting the location

NOTICE! If an error occurs, the objects in the immediate proximity of the device will be damaged.

In accordance with recommendations in EN 61010-2-020, leave a safety clearance of 30 cm around the device during operation.

Please remove all materials and objects from this area.

NOTICE! Damage from overheating.

Do not install the device near heat sources (e.g. heating, drying cabinet).

Do not expose the device to direct sunlight.

Ensure unobstructed air circulation. Maintain a clearance of at least 30 cm (11.8 in) around all ventilation grilles.

Select the location for the device according to the following criteria:

•Suitable power connection as per the name plate (230 V/120 V/100 V).

•Stable, horizontal and resonance-free lab bench.

•A well ventilated environment which is protected from direct sunlight to prevent the device from heating up more.

4.2Preparing installation

Prerequisites

The weight of the centrifuge is 30.0 kg (66.14 lb). For unpacking and installation the assistance of another person is required.

Perform the following steps in the sequence described.

1.Open the box.

2.Remove the accessories.

3.The centrifuge must be lifted from the box by two people.

4.Remove the transport securing device from the sides.

5.Place the device on a suitable lab bench.

Do not use the opening for the condensation water tray as a handle.

6. Remove the plastic sleeve.

Installation

22Centrifuge 5427 R English (EN)

4.3Installing the instrument

Prerequisites

The device is on a suitable lab bench.

NOTICE! Damage to electronic components due to condensation.

Condensate can form in the device after it has been moved from a cool environment to a warmer environment.

After installing the device, wait for at least 4 h. Only then connect the device to the mains power supply.

NOTICE! Centrifuge 5427 R: compressor damage after improper transport.

Only switch on the centrifuge 4 h after installation.

Perform the following steps in the sequence described.

1.Let the device warm up to ambient temperature.

2.Check that the mains voltage and frequency match the requirements on the device type plate.

3.Connect the centrifuge to the mains and switch it on using the mains/power switch.

•Display is active.

•Lid opens automatically

4.Remove the transport securing device of the lid latch.

5.Remove the transport securing device of the motor shaft.

6.Use the details included in the scope of delivery to check that the delivery is complete.

7.Check all parts for any transport damage. Contact your dealer if any damage is found.

8.Insert the condensation water tray into the holder provided (see Fig. 2-1 on p. 7).

Retain the packaging material and the transport protection device for subsequent transport or storage. See also the instructions relating to transport (see p. 45).

Operation

Centrifuge 5427 R 23

English (EN)

5Operation

5.1Overview of operating controls

Before using the Centrifuge 5427 R, familiarize yourself with the display and operating controls.

1	2	3	4	5
	time	temp	speed

10

10

9

8

7

6

Fig. 5-1: Operator panel of the Centrifuge 5427 R

1	Call and select the menu parameters	7	Set speed of centrifugation
	(see Menu navigation on p. 25)	8	Set temperature
2	Switch display of centrifugation speed (rpm or	9	Set centrifugation time
	rcf)
3	Display	10	Start FastTemp temperature control run
			(see Start FastTemp on p. 31)
4	Short spin centrifugation	11	Select menu item
	(see Short spin centrifugation on p. 34)
			(see Menu navigation on p. 25)
5	Release lid
6	Start and stop centrifugation

Please also read the precise description of the individual menu functions (see p. 25).

Operation

24Centrifuge 5427 R English (EN)

1

2

3

4

5

6

7

9	8

Fig. 5-2: Display of the Centrifuge 5427 R.

1 Centrifugation time

2Key lock

Key lock. Centrifugation parameters cannot be accidentally changed.

No key lock. Centrifugation parameters can be changed.

3Start of run time: ATSET function

Start of run time after reaching 95 % of the specified g-force (rcf) or speed (rpm).

Immediate start of run time.

4 Temperature

5Soft ramp

Rotor accelerates and brakes slowly.

No symbol: fast acceleration and braking of rotor.

6g-force (rcf) or speed (rpm)

(see Rotors on p. 9)

7Status of centrifuge

: Centrifuge lid unlocked.

: Centrifuge lid locked.

(Flashing): Centrifuging in progress.

8Display of centrifugation speed

rcf g-force (relative centrifugal acceleration). rpm speed (revolutions per minute).

9 Status of speaker

Speaker switched on. Speaker switched off.

Operation

Centrifuge 5427 R 25

English (EN)

5.2Menu navigation

Proceed as follows to change settings in the device menu:

1. Open the menu.

2. Select the desired menu item.

	3.	Confirm your selection.
	4.	Select the setting of the corresponding parameters.
	5.	Confirm the changed setting.
		The BACK menu item belonging to the first menu level appears.
	6.	Exit the menu.

To exit the second menu level without changing a parameter, select the BACK menu item and confirm with menu/enter.

5.3Menu

Tab. 5-1: Menu structure of the Centrifuge 5427 R.

Menu level 1 (M 1)	Menu level 2 (M 2)			Display
SOFT	On	Rotor accelerates and brakes slowly.
Soft ramp: decreases acceleration speed	OFF	Rotor accelerates and brakes rapidly.
and braking speed
Disabled with short spin centrifugation
RAD	0_2ML	Select the radius according to the adapter
For the internal conversion of speed (rpm)	0_4ML	used.
to g-force (rcf), the radius is dependent on	0_5ML	Only rotor FA-45-12-17:
the adapter used.	0_6ML	MAX
		1_5/2
		HPLC
		CRYO
	MAX	Largest radius of the used rotor.
LOCK	On	Set the centrifugation parameters
Key lock: centrifugation parameters		permanently. SAFE appears in the display
(temperature, g-force (rcf) or speed (rpm))		when the time, temp or speed keys are
cannot be accidentally changed.		pressed.
	OFF
ATSET	On	The set run time will be counted down
Set start of centrifuging run time.		after 95 % of the specified	g-force (rcf) or
		speed (rpm) has been reached.
	OFF	The set time is counted down immediately.

Operation

26Centrifuge 5427 R English (EN)

Menu level 1 (M 1)	Menu level 2 (M 2)			Display
SHORT	MAX	Short spin centrifugation at the maximum
Set the speed of the short spin		speed of the inserted rotor
centrifugation.	Set	Short spin centrifugation with set speed
No SOFT function with short spin
		(g-force (rcf) or speed	(rpm)).
centrifugation.
Temp	8 h	Default setting: Continuous cooling ends
Set the time limit for continuous cooling		after 8 h.
("ECO shut-off") (see p. 30).	1 h	To limit continuous cooling after a run to
	2 h	1 h, 2 h, or 4 h, the centrifuge lid must be
	4 h	opened and closed again after the run.
	oo	Endless operation of continuous cooling.
Alarm	On	Switch on loudspeaker.
Switch the loudspeaker on or off.	OFF	Switch off loudspeaker.
VOL	VOL1	The volume of the loudspeaker can be set
Set the volume.	…	to 5 levels (VOL1 to VOL5). The
	VOL5	loudspeaker must be switched on for the
		adjustment to be audible.
SLEEP	On	Standby mode activated.
Switch the standby mode on or off.	OFF	Standby mode deactivated.
If the centrifuge has not been used for
15 min, it will switch to standby. EP then
appears in the display.
Press a key or close the centrifuge lid to
exit the standby mode.

The Back menu item can also be found in both menu levels.

Back in menu level 2: return to menu level 1.

Back in menu level 1: exit menu.

Operation

Centrifuge 5427 R 27

English (EN)

5.4Preparing for centrifugation

5.4.1 Switching on the centrifuge

Switch the centrifuge on using the mains/power switch.

The centrifuge lid opens automatically after switching on using the mains/power switch. The parameter settings of the last run are displayed.

5.4.2 Inserting the rotor

1.Fit the rotor vertically on the motor shaft.

2.Insert the supplied rotor key into the rotor nut.

3.Turn rotor key clockwise until the rotor nut is firmly tightened.

5.4.3Automatic rotor detection

The centrifuge has automatic rotor detection. It detects a newly inserted rotor and displays the name of the rotor for 2 s. g-force (rcf) and speed (rpm) are automatically limited to the maximum permitted value for the rotor.

Manually turn the rotor counterclockwise to trigger rotor detection.

•The name of the rotor appears in the display.

•The g-force (rcf) and speed (rpm) are automatically limited to the maximum permissible value for the rotor.

5.4.4Replacing the rotor

1.Turn the rotor nut counterclockwise using the supplied rotor key.

2.Remove the rotor by lifting it vertically.

3.Vertically place another rotor on the motor shaft.

4.Insert the supplied rotor key into the rotor nut.

5.Turn rotor key clockwise until the rotor nut is firmly tightened.

6.In order to trigger the rotor detection, turn the rotor counterclockwise by hand.

•The name of the rotor appears in the display.

•The g-force (rcf) and speed (rpm) are automatically limited to the maximum permissible value for the rotor.

Rotor detection can also be triggered by short spin centrifugation:

Press the short key until the name of the rotor appears in the display.

Error message after rotor change

• If you start centrifuging immediately after a rotor change, the centrifuge has not carried out an automatic rotor detection yet. The speed set for the previous rotor may exceed the maximum permitted speed for the new rotor. In this case, the centrifuge stops after the automatic rotor detection and displays SPEED. The new maximum permitted speed appears in the display. You can then restart the centrifuging with this setting or adjust the speed as necessary.

After each rotor change, check whether the new rotor is detected by the device. Check the set g-force (rcf) or speed (rpm) and adjust it if necessary.

Operation

28Centrifuge 5427 R English (EN)

5.4.5Loading the rotor

5.4.5.1 Loading a fixed-angle rotor

CAUTION! Risk of injury due to asymmetric loading of a rotor.

Load rotors symmetrically with identical tubes.

Only load adapters with suitable tubes.

Always use the same type of tubes (weight, material/density and volume).

Check symmetric loading by balancing the adapters and tubes used with scales.

CAUTION! Risk from damaged or overloaded tubes.

When loading the rotor, observe the safety precautions on dangers as a result of overloaded or damaged tubes (see Warnings for intended use on p. 17).

Use matching rotor lids

• Fixed-angle rotors may only be operated with the appropriate rotor lid in each case. The rotor name on the rotor must be the same as the name on the rotor lid.

•To carry out an aerosol-tight centrifugation, an aerosol-tight rotor (label: red ring) and the corresponding aerosol-tight rotor lid (label: aerosol-tight and red lid screw) must be used.

The device automatically detects imbalances during operation and stops the run immediately with an error message and a signal tone.

Check the load, balance the tubes and restart the run.

To load the rotor, proceed as follows:

1.Check the maximum payload (adapter, tube and contents) for each rotor bore.

The information about this can be found on every rotor and in this operating manual (see Rotors on p. 9).

2.Load rotors and adapters only with the tubes intended for them.

3.Insert sets of two tubes in the opposite bores of the rotor. To ensure symmetric loading, tubes that are arranged opposite each other must be of the same type and contain the same filling quantity.

To minimize weight differences between filled sample tubes, we recommend taring with a scale. This will reduce wear on the drive and reduce running noise.

Operation

Centrifuge 5427 R 29

English (EN)

Spin columns

For centrifuging spin columns in the rotor FA-45-24-11-Kit, you can leave the tube lids open. However, this can only be done using the tubes provided by kit manufacturers for this purpose. For reliable centrifugation, you must lean the open tube lids against the edge of the rotor. Ensure that the lids do not protrude past the edge of the rotor in the process, then attach the matching rotor lid.

5.4.5.2 Loading a swing-bucket rotor

Prerequisites

•Use a rotor, bucket and adapter combination that is approved by Eppendorf.

•The carriers are sorted by weight category. Carriers located opposite each other must belong to the same weight category. This is laterally engraved in the groove: e.g., 68 (the last two digits in grams). For repeat orders, please specify the weight class.

•Matched and checked tubes

To load the rotor, proceed as follows:

1.Check the bucket grooves for cleanliness and lightly lubricate them with pivot grease (int. order no.: 5810 350.050 / North America: 022634330).

Contaminated grooves and pegs prevent a uniform swinging of the bucket.

2.Hang the buckets into the rotor.

All rotor positions must be loaded with carriers.

3.Check that all carriers are hanging properly and can swing freely.

4.Check the maximum payload (adapter, tube and contents) for each bucket.

The details on the maximum payload can be found on the rotor and in this operating manual (see Rotors on p. 9).

5.Load the buckets symmetrically.

Fig. 5-3: Incomplete, but symmetric loading of the buckets. The pegs of each bucket must be uniformly loaded.

The supply shown on the right side is incorrect because it does not allow the bucket to properly swing out.

Operation

30Centrifuge 5427 R English (EN)

5.4.6 Closing the rotor lid

1.Only QuickLock rotor lid: check that the external sealing ring correctly fits in the groove.

2.Vertically fit the rotor lid on the rotor.

3.Turn the rotor lid screw clockwise to seal the rotor.

Only QuickLock rotor lid:

Turn the rotor lid screw clockwise as far as it will go, until an audible "click" can be heard. The rotor is not properly closed until an audible click is heard!

5.4.7Closing the centrifuge lid

WARNING! Risk of injury when opening or closing the centrifuge lid.

There is a risk of crushing your fingers when opening or closing the centrifuge lid.

When opening or closing the centrifuge lid, do not reach between the lid and device or into the latching mechanism of the lid.

Always open the centrifuge lid completely to prevent it from falling.

1.Check the correct attachment of the rotor and rotor lid.

2.Press the centrifuge lid down until it is gripped by the lid latch.

The lid will be automatically closed and the locking mechanism can be clearly heard.

The open key lights up blue. The display shows the symbol.

5.5Cooling

The centrifuge cools or maintains the set temperature is the following requirements are met:

•The centrifuge is switched on.

•The centrifuge lid is closed.

•Only with continuous cooling: The set temperature is lower than the ambient temperature.

If the rotor stops (continuous cooling), cooling is slower than during centrifugation or a temperature control run.

5.5.1		Set the temperature
1.	To set the temperature, use the temp arrow keys to select a temperature between -11 °C and 40 °C.
2.	Set the run time and g-force (rcf) or speed (rpm). Press the start/stop key to start the centrifugation.
The temperature can be changed during centrifugation.
5.5.2		Temperature display
Temperature display if the rotor stops			Set temperature
Temperature display during centrifugation			Actual temperature