Eppendorf 5424 R User Manual

Centrifuge 5424/5424 R

Operating manual

Copyright © 2011 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner.

Trademarks

eppendorf is a registered trademark of Eppendorf AG, Hamburg, Germany. Microtainer is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.

Trademarks are not marked in all cases with ™ or ® in this manual.

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Operating manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Declarations and Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176

5404 900.023-04/122011

You will find a detailed description of these figures in your language in Chapters 1.1/2.1 and 4.1/5.1.

	1
1	2
2

		5
	3	6
	4
		7

				8	9
Fig. 1:		Front and rear view of the Centrifuge 5424
			1
			2
1
2
			5
3
4			7
			9
10			8
			6
11
Fig. 2:		Front and rear view of the Centrifuge 5424 R
1	Centrifuge lid		2	Window
3	Display		4	Control panel
5	Emergency release		6	ID plate
7	Mains switch		8	Power connection
9	Fuse holder		10 USB port
				(only 5424 R, only for technical support)
11 Condensation water tray (only 5424 R)

Fig. 3:

Control panel of the Centrifuge 5424 / 5424 R

1

Call and select the menu parameters

2

Switch the centrifuging speed displayed (rpm/rcf)

3

Display

4

Short Spin centrifugation

5

Release lid

6

Start and stop centrifugation

7

Set the speed of centrifugation

8

Adjust the temperature (only 5424 R)

Dependent on device version designed as a key or

dial.

9

Adjust the centrifuging duration

10

Start the temperature control run fast temp

Dependent on device version designed as a key or

(only 5424 R)

dial.

11

Select the menu item

Fig. 4:		Display of the Centrifuge 5424 R. The display of the Centrifuge 5424 is similar.
1	Centrifuging duration		2	Status of the key lock (LOCK)
3	Status of the function ATSET		4	Temperature (only 5424 R)
5	Soft ramp		6	g-force (rcf) or speed (rpm)
7	Status of the centrifuge		8	Status of the centrifugation speed display
9	Status of the loudspeaker

Centrifuge 5424 / 5424 R — Operating manual

Table of contents

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .			7
1.1	Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		7
1.2	Warning signs and hazard icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		7
	1.2.1	Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	7
	1.2.2	Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	7
1.3	Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		7

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.2.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.2.2 Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.4 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 2.4.1 Rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3 Safety	. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	12
3.1	Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	12
3.2	User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	12
3.3	Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	12
	3.3.1 Declaration concerning the ATEX directive (94/9/EC). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	12
	3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	12
3.4	Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	13
3.5	Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	13
	3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	13
	3.5.2 Incorrect handling of the centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	14
	3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	14
	3.5.4 Extreme strain on the centrifuging tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	15
	3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	16
3.6	Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	16

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 4.2 Installing the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5 Operation . . .		. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	18
5.1	Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		18
5.2	Device menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		20
5.3	Menu navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		21
5.4	Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		21
	5.4.1	Switch on centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	21
	5.4.2	Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	21
	5.4.3	Load the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	21
	5.4.4	Close centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	23
5.5	Cooling (only 5424 R). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		23
	5.5.1	Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	23
	5.5.2	Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	23
	5.5.3	Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	23
	5.5.4	Fast Temp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	23
	5.5.5	Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	24
5.6	Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		24
	5.6.1 Centrifuging with time preset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		25
	5.6.2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		25
	5.6.3	Short spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	26
	5.6.4	Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	26

EN

manual Operating

5

EN

Operating manual

Centrifuge 5424 / 5424 R — Operating manual

Table of contents

6	Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		27
	6.1	Prepare cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	27
	6.2	Perform cleaning / disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	27
		6.2.1 Clean / disinfect device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	28
		6.2.2 Clean / disinfect rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	28
	6.3	Additional service instructions for Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	29
	6.4	Glass breakage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	29
	6.5	Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	29
	6.6	Decontamination before shipping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	29
7	Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .		30
	7.1	General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	30
	7.2	Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	31
	7.3	Emergency release. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	32
8 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .			33
	8.1	Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	33
	8.2	Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	33
	8.3	Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .	33

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

9.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 9.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 9.3 Weight / dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 9.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 9.4.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 9.4.2 Centrifuge 5424 R. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

10 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

10.1 Centrifuge 5424 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 10.2 Centrifuge 5424 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 10.3 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 10.3.1 Rotors and rotor lids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 10.3.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 10.3.3 Other accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

6

Centrifuge 5424 / 5424 R — Operating manual

1 User instructions

1.1Using this manual

Please read this operating manual completely before using the device for the first time.

Please view this operating manual as part of the product and keep it somewhere easily accessible.

When passing the device on to third parties, be sure to include this operating manual.

If this manual is lost, please request another one. The current version can be found on our website www.eppendorf.com.

The Centrifuge 5424 / 5424 R is available in two versions: keypad or rotary knobs. This operating manual generally describes how to operate the keypad version. However, it also applies to the rotary knob version.

1.2Warning signs and hazard icons

1.2.1Hazard symbols

Biohazard

Explosion

Electric shock

Crushing

Hazard point

Material damage

1.2.2Degrees of danger

The degree of danger is a part of a safety note and distinguishes the possible results of non-observance from each other.

DANGER	Will lead to severe injuries or death.
WARNING	May lead to severe injuries or death.
CAUTION	May lead to light to moderate injuries.
NOTICE	May lead to material damage.

1.3Abbreviations used

PCR Polymerase chain reaction PTFE Polytetrafluorethylen

rcf Relative centrifugal force rpm Revolutions per minute UV Ultraviolet radiation

EN

manual Operating

7

EN

Operating manual

Centrifuge 5424 / 5424 R — Operating manual

2Product description

2.1Main illustration

The depiction of the Centrifuge 5424 / 5424 R can also be found on the front fold-out page (see Fig. 1 and Fig. 2).

	1
1	2
2

		5
	3	6
	4
		7

					8	9
Fig. 1:		Front and rear view of the Centrifuge 5424
			1
			2
1
2
			5
3
4			7
			9
10			8
			6
11
Fig. 2:		Front and rear view of the Centrifuge 5424 R
1	Centrifuge lid			2	Monitoring glass
					Visual control for rotor stop or option for speed control
					via stroboscope.
3	Display			4	Control panel
	Depiction of the current centrifuging parameters and				Keys and dials (dependent on the device version) for
	device settings (see Fig. 4 on p. 19).				operating the centrifuge (see Fig. 3 on p. 18).
5	Emergency lid release			6	ID plate
	(see Emergency release on p. 32)
7	Mains switch			8	Mains connection
	Switch for switching the device on and off.				Connection socket for the mains cable supplied.
	Switch position 0: The device is switched off.
	Switch position I: The device is switched on.
9	Fuse holder			10	USB port (only 5424 R)
					Interface for error analysis and software updates by
					the Technical Service.
11	Condensation water tray (only 5424 R)

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Centrifuge 5424 / 5424 R — Operating manual

2Product description

2.2Delivery package

2.2.1Centrifuge 5424

Quantity	Order No.	Order No.	Description
	(International)	(North America)
			Centrifuge 5424
			See chapter Ordering Information for corresponding device version,
1	-	-	equipment and order number
			Fuses
1	5424 852.122	950004266	3.15 AT for 230 V, 2 pieces
or	5424 852.130	950004240	6.3 AT for 120 V/100 V, 2 pieces
			Rotor key
1	5416 301.001	022634305	Standard
			Captain Eppi rotor key holder
1	5703 350.102	022639609	1 piece
1	-	-	Power cable
			Operating manual Centrifuge 5424/5424 R
1	5404 900.023	5404900023	Languages: EN, DE, FR, ES, IT, PT
1	5404 900.031	5404900031	Languages: DA, FI, EL, NL, SV (230 V devices only)

2.2.2Centrifuge 5424 R

Quantity	Order No.	Order No.	Description
	(International)	(North America)
			Centrifuge 5424 R
			See chapter Ordering Information for corresponding device version,
1	-	-	equipment and order number
			Fuses
1	5424 852.122	950004266	3.15 AT for 230 V, 2 pieces
or	5424 852.130	950004240	6.3 AT for 120 V/100 V, 2 pieces
			Rotor key
1	5416 301.001	022634305	Standard
1	-	-	Power cable
1	5404 850.085	5404850085	Tray for condensation water
			Operating manual Centrifuge 5424/5424 R
1	5404 900.023	5404900023	Languages: EN, DE, FR, ES, IT, PT
1	5404 900.031	5404900031	Languages: DA, FI, EL, NL, SV (230 V devices only)

2.3Features

The versatile Centrifuge 5424 / 5424 R has a capacity of 24 x 2 mL and reaches a maximum of 21,130 x g / 15,000 rpm (5424: 120 V, 5424 R: 230 V, 120 V, 100 V) or 20,238 x g / 14,680 rpm (5424: 230 V, 100 V). You can select between four different rotors to centrifuge the following tubes for your various applications:

•Micro test tubes (0.2 to 2.0 mL)

•PCR strips

•Microtainers (0.6 mL)

•Spin columns (1.5/2.0 mL)

The Centrifuge 5424 R has an additional temperature control function for centrifugation between -10°C and +40°C. The fast temp function can be used to start a temperature control run without samples to adjust the rotor chamber quickly to the set target temperature.

EN

manual Operating

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EN

Operating manual

Centrifuge 5424 / 5424 R — Operating manual

2Product description

2.4Rotors

You can operate the Centrifuge 5424 / 5424 R with the following rotors. Before use of sample tubes, please note the manufacturer's specifications with regard to centrifugation resistance (max. rcf).

		Max. capacity		Max. g-force (rcf) /	Max. g-force (rcf) or	Notes
				speed (rpm)	speed (rpm)
				without adapter	without adapter
				(5424: 120 V,	(5424: 230 V/100 V)
				5424 R)
				Max. load per rotor bore(1)
	Rotor	24 micro test tubes of 1.5/2.0 mL		21,130 x g /	20,238 x g /	•	Aerosol-tight(2) rotor
	FA-45-24-11	each or spin columns.		15,000 rpm	14,860 rpm		lid (aluminum).
		With adapters:				•	Spin columns
				3.75 g
		•	0.2 mL PCR tubes				possible, better with
		•	0.4 mL/0.5 mL micro test				rotor F-45-18-11-Kit.
			tubes
		•	0.6 mL Microtainers
	Rotor	24 micro test tubes of 1.5/2.0 mL		21,130 x g /	20,238 x g /	•	Aerosol-tight(2) rotor
	FA-45-24-11-	each or spin columns.		15,000 rpm	14,860 rpm	•	lid (aluminum).
	Special	With adapters:					PTFE-coated
				3.75 g
		•	0.2 mL PCR tubes				(particularly resistant
		•	0.4 mL/0.5 mL micro test				to chemicals),
							marked: coated
			tubes
						•	Spin columns
		•	0.6 mL Microtainers
							possible, better with
							rotor F-45-18-11-Kit.
	Rotor	18 spin columns or 1.5/2.0 mL		18,111 x g /	17,347 x g /	•	Particularly high edge
	F-45-18-11-	micro test tubes.		15,000 rpm	14,860 rpm		for all commercially
	Kit	With adapters:					available spin
				3.75 g
							columns. See the note
		•	0.2 mL PCR tubes
							about centrifugation
		•	0.4 mL/0.5 mL micro test
							with open tube lids in
			tubes				this regard(see Load
		•	0.6 mL Microtainers				the rotor on p. 21).
	Rotor	Four PCR strips of 5/8 or		18,615 x g /	17,829 x g /
	F-45-32-5-	32 PCR tubes of 0.2 mL each.		15,000 rpm	14,860 rpm
	PCR
				3.5 g

(1)Maximum load per rotor bore for adapter + tube + content.

(2)Aerosol tightness tested and certified by the Centre for Emergency Preparedness and Response, Health Protection Agency, Porton Down (UK) (see certificates at the end of this operating manual).

For the rotors and rotor lids labeled coated, color fluctuations may occur as a result of the production process. These fluctuations have no effect on service life or resistance to chemicals.

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Centrifuge 5424 / 5424 R — Operating manual

2 Product description

2.4.1Rcf display and calculation

Use the rpm/rcf key to switch the display of centrifugation speed between speed (rpm) and g-force (rcf). Ensure that the g-force displayed upon switching is standardized to suit the rotor FA-45-24-11 without an adapter. When other rotors and adapters are used, you can achieve the following maximum g-forces (rcf) at the maximum speed (see p. 10):

	Rotor	Adapters	Max. centrifugation	Max. g-force (rcf)	Max. g-force (rcf)
			radius rmax [cm]	(5424 120 V, 5424 R)	(5424 230 V/100 V)
	FA-45-24-11,	without adapter	8.4	21,130	20,238
	FA-45-24-11-Special
		0.2 mL	6.3	15,848	15,179
		0.4 mL	8.4	21,130	20,238
		0.5 mL	7.3	18,363	17,558
		0.6 mL	8.4	21,130	20,238
	F-45-18-11-Kit	without adapter	7.2	18,111	17,347
		0.2 mL	5.1	12,829	12,288
		0.4 mL	7.2	18,111	17,347
		0.5 mL	6.1	15,345	14,697
		0.6 mL	7.2	18,111	17,347
	FA-45-32-5-PCR	without adapter	7.4	18,615	17,829

To determine the g-force (rcf) for a specific adapter, you can calculate per DIN 58 970 using the following formula:

rcf = 1.118 · 10-5 · n2 · rmax

n: revolutions per minute (rpm) rmax: max. centrifuging radius in cm

Example

In rotor FA-45-24-11, the 0.5 mL adapter has a maximum radius of 7.3 cm. At 7,000 rpm a maximum g-force of 4,000 x g is reached.

EN

manual Operating

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EN

Operating manual

Centrifuge 5424 / 5424 R — Operating manual

3Safety

3.1Intended use

The Centrifuge 5424 / 5424 R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes.

Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable for any

CAUTION! damage resulting from the use of non-recommended accessories and spare parts or from the improper use of such equipment.

Only use accessories and original spare parts recommended by Eppendorf.

3.2User profile

This device may only be operated by correspondingly trained specialist staff. This staff must have carefully read the operating manual and be familiar with the function of the device.

3.3Application limits

3.3.1Declaration concerning the ATEX directive (94/9/EC)

Danger of explosion!

Do not operate the device in areas where work is being carried out with explosive substances.

Do not use this device to process any explosive, radioactive or highly reactive substances.

DANGER! Do not use this device to process any substances which could create an explosive atmosphere.

Due to its design and the environmental conditions on the inside of the device, the Centrifuge 5424 / 5424 R is not suitable for use in a potentially explosive atmosphere.

The device must therefore only be used in a safe environment, such as in the open environment of a ventilated laboratory or an extractor hood. The use of substances which may contribute to a potentially explosive atmosphere is not permitted. The final decision with regard to the risks connected with the use of such substances is the responsibility of the user.

3.3.2Maximum service life for accessories

Risk of injury from chemically or mechanically damaged accessories.

Even small scratches and cracks can lead to serious internal material damage.Protect all parts from mechanical damage.

CAUTION! Check accessories regularly.

Do not use rotors or buckets with signs of corrosion or mechanical damage (e.g. deformations).

Do not use accessories whose maximum service life has been exceeded.

Accessory	Maximum service life from first
	commissioning
Transparent polypropylene rotor lids	3 years
Plastic adapters	1 year

12

Centrifuge 5424 / 5424 R — Operating manual

3 Safety

For the other rotors and rotor lids of this centrifuge (see Rotors on p. 10) there is no limit for their service life, as long as the following conditions are met: proper use, recommended maintenance and undamaged condition. Lids of aerosol-tight rotors must be replaced after 50 autoclave cycles to ensure aerosol tightness.

The date of manufacture is stamped on the rotors in the format 03/07 (= March 2007) or on the inside of the plastic rotor lids in the form of a clock . This is for information only and does not have any reference to the service life.

3.4Information on product liability

In the following cases, the protection provided by the device may be impaired. The liability for the function of the device passes to the operator if:

•The device is not used in accordance with the operating manual.

•The device is used outside of the range of application described in the preceding chapters.

•The device is used with accessories or consumables (e.g., tubes) which are not recommended by Eppendorf.

•The device is maintained or repaired by persons not authorized by Eppendorf.

•The owner has made unauthorized modifications to the device.

3.5Warnings for intended use

Read the operating manual first and observe the following general safety instructions before using the Centrifuge 5424 / 5424 R.

3.5.1Personal injury or damage to the equipment

Risk of electrical shock from damage to the device or mains cable.

The device may only be switched on if the device and mains cable are undamaged.

Only use devices which have been professionally installed or repaired.

WARNING!

	Lethal voltages inside the device.
	Ensure that the housing is always closed and undamaged so that no parts inside the device
	can be contacted by accident.
WARNING!	Do not remove the housing of the device.
	Do not allow any liquids to enter the inside of the housing.
	Do not allow the device to be opened by anyone except service personnel who have been
	specifically authorized by Eppendorf.

Danger from using an incorrect power supply.

Only connect the device to voltage supplies which correspond with the electrical requirements on the name plate.

WARNING!

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manual Operating

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EN

Operating manual

Centrifuge 5424 / 5424 R — Operating manual

3 Safety

Damage to health when handling infectious liquids and pathogenic germs.

Observe the national regulations for handling these substances, the biological security level of your laboratory, the material safety data sheets and the manufacturer's application notes.

	WARNING!	Use suitable aerosol-tight closure systems for the centrifugation of these substances.
		When working with pathogenic germs belonging to a higher risk group, more than one
		aerosol-tight bioseal must be provided for.
		Wear personal protective equipment (PPE).
		Follow the instructions regarding hygiene, cleaning and decontamination.
		For complete instructions regarding the handling of germs or biological material of risk group
		II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
		Organization, current edition of the Laboratory Biosafety Manual).
		Centrifuge lid can crush. Keep hands clear.
		When opening or closing the device lid, do not reach between the lid and device or into the
		latching mechanism of the lid.
	WARNING!	Always open the centrifuge lid completely to prevent it from falling.
		Damage to device by spilling liquids in the rotor or rotor chamber
		1. Switch the device off.
	NOTICE!	2. Disconnect the device from the power supply.
		3. Clean the device and the accessories carefully in accordance with the cleaning and
		disinfection instructions in the operating manual.
		4. If a different cleaning and disinfecting method is to be used, contact Eppendorf to ensure that
		the intended method will not damage the device.
		Damage to electronic components through formation of condensation.
		After the device has been moved from a cool to a warmer environment, condensation can form
		inside the device.
	NOTICE!	Wait at least three hours (5424) or four hours (5424 R) before connecting the device to the
		power supply.

Only 5424: Alternatively: let the device run for half an hour before transporting it.

3.5.2Incorrect handling of the centrifuge

	Damage from knocking against or moving the device during operation.
	A rotor banging against the rotor chamber wall can cause considerable damage to the device and
	rotor.
NOTICE!	Do not move or knock against the device during operation.

3.5.3Incorrect handling of the rotors

	Risk of injury from improperly attached rotors and rotor lids.
	Centrifuge only with the rotor and rotor lid firmly tightened.
	If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
CAUTION!	properly secured. Stop centrifugation immediately by pressing the start/stop key.

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