Metrohm 845 User Manual
845 Eluent Synthesizer
Handbuch
8.845.8001EN
Metrohm AG
CH-9100 Herisau
Switzerland
Phone +41 71 353 85 85
Fax +41 71 353 89 01
info@metrohm.com
www.metrohm.com
845 Eluent Synthesizer
8.845.8001EN
Handbuch
08.2008 zst
Teachware
Metrohm AG
CH-9100 Herisau
teachware@metrohm.com
This documentation is protected by copyright. All rights reserved.
Although all the information given in this documentation has been
checked with great care, errors cannot be entirely excluded. Should you
notice any mistakes please send us your comments using the address
given above.
Documentation in additional languages can be found on
http://documents.metrohm.com.
Contents
Table of contents
1 Introduction.................................................... 1
1.1 Instrument description ............................................................. 1
1.2 Parts and controls .................................................................... 1
1.2.1 Front of 2.845.0010 .......................................................................2
1.2.2 Front of 2.845.0020 .......................................................................3
1.2.3 Rear view .......................................................................................4
1.3 Information on the Instructions for Use .................................. 6
1.3.1 Organization ..................................................................................6
1.3.2 Notation and pictograms ..............................................................7
1.4 Safety information..................................................................... 8
1.4.1 Electrical safety..............................................................................8
1.4.2 General precautionary rules .......................................................... 8
2 Installation ..................................................... 9
2.1 Setting up the instrument ......................................................... 9
2.1.1 Packaging...................................................................................... 9
2.1.2 Check.............................................................................................9
2.1.3 Location ......................................................................................... 9
2.2 Mains connection...................................................................... 9
2.3 Connection to PC.................................................................... 10
2.4 Dosino(s)/Stirrer connections................................................ 11
2.5 Tubing connections ................................................................ 11
2.5.1 Instrument with tubing connections ............................................12
2.5.2 Connecting the drainage tubing .................................................13
2.5.3 Connections to bottle adapter ....................................................13
2.5.4 Connecting the components.......................................................13
2.6 pH Controller........................................................................... 14
2.6.1 Tubing connection for pH Controller........................................... 14
2.6.2 pH Controller cable connection ..................................................15
3 «Mix Control» software................................. 16
3.1 Introduction to the software ................................................... 16
3.1.1 The principle of «Mix Control» .....................................................16
3.1.2 Procedure after the first start of «Mix Control» ............................16
3.2 Main Window........................................................................... 16
3.2.1 Menus..........................................................................................16
3.2.2 Description of the symbols .........................................................19
3.3 Method..................................................................................... 20
3.3.1 General information about methods ...........................................20
3.3.2 Parameters ..................................................................................20
3.3.3 pH Adjustment.............................................................................21
3.3.4 Creating a method.......................................................................23
3.4 Configuration .......................................................................... 23
3.4.1 Depot ........................................................................................... 23
3.4.2 Components................................................................................25
3.4.3 System Settings........................................................................... 26
3.4.4 Report Configuration ...................................................................27
3.4.5 User Administration ..................................................................... 29
3.4.6 Rinse............................................................................................33
3.5 Extras....................................................................................... 36
3.5.1 Sign methods ..............................................................................36
845 Eluent Synthesizer / Instructions for Use 8.845.8001EM
I
Contents
3.5.2 Audit trail...................................................................................... 37
3.5.3 Window "Report List" ................................................................... 39
3.5.4 GLP Manager .............................................................................. 41
3.5.5 Firmware Update......................................................................... 43
3.5.6 Login Service............................................................................... 44
4 Maintenance - Troubleshooting...................45
4.1 Care .........................................................................................45
4.1.1 Decommissioning .......................................................................45
4.2 Dosing units ............................................................................ 45
4.2.1 Deinstallation............................................................................... 45
4.2.2 Cleaning ...................................................................................... 46
4.2.3 Installation ...................................................................................47
4.3 Troubleshooting...................................................................... 49
5 Annex ............................................................50
5.1 Technical data......................................................................... 50
5.1.1 Interfaces..................................................................................... 50
5.1.2 Mains connection........................................................................ 50
5.1.3 Safety specifications ...................................................................50
5.1.4 Electromagnetic compatibility (EMC) .........................................50
5.1.5 Ambient temperature ..................................................................51
5.1.6 Dosing drive ................................................................................ 51
5.1.7 Dosing Unit.................................................................................. 51
5.1.8 Reference conditions .................................................................. 51
5.1.9 Dimensions ................................................................................. 51
5.2 Standard equipment ............................................................... 52
5.2.1 Standard equipment for 2.845.0010........................................... 52
5.2.2 Standard equipment for 2.845.0020........................................... 54
5.2.3 Accessories for pH adjustment (optional) .................................. 57
5.2.4 Optional accessories ..................................................................61
5.2.5 Producers of concentrates and standards ................................. 62
5.2.6 Systems for producing ultra pure water ..................................... 62
5.3 Warranty .................................................................................. 63
5.3.1 Warranty ......................................................................................63
5.3.2 Declaration of Conformity ...........................................................64
5.3.3 Quality Management Principles .................................................. 65
5.3.4 Index............................................................................................ 66
List of figures
Figure 1: Front of 2.845.0010 ....................................................................... 2
Figure 2: Front of 2.845.0020 ....................................................................... 3
Figure 3: Rear view of 845 Eluent Synthesizer ............................................. 4
Figure 4: Tubing connections..................................................................... 12
Figure 5: Dosing unit attachment ............................................................... 46
Figure 6: Connections with one Dosino ..................................................... 47
Figure 7: Connections with two Dosinos.................................................... 48
845 Eluent Synthesizer / Instructions for Use 8.845.8001EN
II
1.1 Instrument description
1 Introduction
The 845 Eluent Synthesizer and its associated «Mix Control» software can be used for the fully automatic preparation of solutions and
mixtures such as eluents, mobile phases, buffer solutions or standards
with the required concentration and component ratios.
The 845 Eluent Synthesizer is based on the Dosino technology which
allows high-precision dosing to be carried out. Both aqueous (acidic or
basic) and organic mixtures can be prepared. The system (with an optional extension) can also be used to adjust the pH.
1.1 Instrument description
Two versions of the instrument are available :
• 2.845.0010 845 Eluent Synthesizer with one Dosino
• 2.845.0020 845 Eluent Synthesizer with two Dosinos
The 2.845.0010 Eluent Synthesizer with one Dosino can be used to mix
together three components (of which one is the main component).
The 2.845.0020 Eluent Synthesizer with two Dosinos can be used to
mix together five components (of which one is the main component).
Both versions contain a built-in magnetic stirrer The software «Mix Control» is supplied with the instrument.
The 2.702.0120 Eluent Synthesizer Package for the automatic pH adjustment is offered as an option. In addition to a 702 Titrino this also
contains an Aquatrode, a 10 mL exchange unit and a Remote Box (see
Section
The 845 Eluent Synthesizer is connected to a PC via a USB and operated with the «Mix Control» PC software provided. The operation of the
software is described in the online help and in Section
structions for Use.
5.2.3).
1.2 Parts and controls
3 of these In-
In this Section you will find the numbers and designations of the parts
and controls of the 845 Eluent Synthesizer. The numbering applies
throughout the Instructions for Use, i.e. bold numbers in the text (e.g.
3
) refer to the parts and controls illustrated here.
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
1
1 Introduction
1.2.1 Front of 2.845.0010
4
3
5
6
2
1
Figure 1: Front of 2.845.0010
LED "Power"
1
Lights up green when the instrument is
switched on.
Place for eluent bottle
2
To place the eluent bottle while preparing the mixture. There is a built-in
magnetic stirrer beneath it.
Holder for bottle adapter
3
Holder for bottle adapter
21.
Dosino 1 outlet
4
Outlet for the components from Dosino
1.
Connections for components
5
Connections for components 1 - 3.
Recesses for magnetic labels
6
For identifying components 1 - 3.
2
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
1.2 Parts and controls
1.2.2 Front of 2.845.0020
7
4
3
5
6
2
1
Figure 2: Front of 2.845.0020
1 LED "Power"
Lights up green when the instrument is
switched on.
2 Place for eluent bottle
The eluent bottle is placed here for
preparing the mixture. There is a builtin magnetic stirrer beneath it.
3 Holder for bottle adapter
Holder for bottle adapter
21.
4 Dosino 1 outlet
Outlet for the components from Dosino
1.
5 Connections for components
Connections for components 1 - 5.
6 Recesses for magnetic labels
For identifying components 1 - 5.
Dosino 2 outlet
7
Outlet for components from Dosino 2.
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
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1 Introduction
1.2.3 Rear view
19
8
9
10
11
12
13
14
Figure 3: Rear view of 845 Eluent Synthesizer
Lead-throughs for Dosino cables
8
Lead-through for stirrer cable
9
Connection "Stirrer / MSB3"
10
For connecting a stirrer (cable marked
with "Stirrer").
15
16
USB-connections USB1 and USB2
11
USB-ports (type A) for connecting devices with a USB cable.
Controller connection
12
For connecting to a PC.
18
17
4
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
1.2 Parts and controls
Connection "MSB1 / Dosino 1"
13
For connecting Dosino 1 (cable
marked with "Dosino 1").
Connection "MSB2 / Dosino 2"
14
For connecting Dosino 2 (cable
marked with "Dosino 2") (only for version 2.845.0020).
Connection "Remote Box / MSB4"
15
For connecting the Remote-Box for pH
adjustment with Titrino(s) (see Section
2.6.2).
Mains connection socket
16
Mains connection (see Section
Device type and serial number
17
Connection for drainage tubing
18
For draining off any liquid that has escaped inside the instrument (see Sec-
2.5.2).
tion
Rear panel
19
2.2).
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
5
1 Introduction
1.3 Information on the Instructions for Use
Please read through these Instructions for Use carefully before you put
the 845 Eluent Synthesizer into operation. The Instructions for Use
contain information and warnings to which the user must pay attention
in order to assure safe operation of the instrument.
1.3.1 Organization
These Instructions for Use 8.845.1003 for the 845 Eluent Synthesizer
provide a comprehensive overview of installation, operation, maintenance, fault rectification and technical specifications of this instrument.
The Instructions for Use are organized as follows:
Sect.
1 Introduction
General description of instrument, parts and controls
and safety notes.
Sect.
2 Installation
Installation of the instrument, mains connection, connection to PC, connection of Dosino(s)/stirrer, pH
controller.
3 «Mix Control» software
Sect.
Explanation of how to use the «Mix Control» software.
Sect.
4 Maintenance - Troubleshooting
Care, cleaning the dosing units, troubleshooting.
Sect.
5 Annex
Technical data, standard equipment, options, warranty, conformity declarations, index.
To find the required information on the instruments, use either the Ta-
ble of contents or the Index at the back.
6
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
1.3 Information on the Instructions for Use
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these Instructions for Use:
12 Part or control
Hazard
This symbol draws attention to a
possible danger to life or of injury if
the associated directions are not
followed correctly.
Warning
This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed correctly.
Caution
This symbol marks important information. First read the associated
directions before you continue.
Comment
This symbol marks additional information and tips.
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
7
1 Introduction
1.4 Safety information
This instrument must only be operated in accordance with the information provided in these Instructions for Use.
1.4.1 Electrical safety
While electrical safety in the handling of the 845 Eluent Synthesizer is
assured in the context of the specifications EN / IEC 61010-1, the following points should be noted:
• Electronic parts
Only qualified Metrohm technicians should carry out service work on
electronic components.
• Protection against electrostatic charges
Electronic components are sensitive to electrostatic charges and can
be destroyed by a discharge.
1.4.2 General precautionary rules
• Handling of solvents
Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.
8
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
2.1 Setting up the instrument
2 Installation
2.1 Setting up the instrument
2.1.1 Packaging
The 845 Eluent Synthesizer is supplied together with the separately
packed accessories in special packagings containing shock-absorbing
foam linings designed to provide excellent protection. Please store all
these special packagings as only they assure transport of the
instrument free from damage.
2.1.2 Check
After receipt, immediately check whether the shipment is complete and
has arrived without damage (compare with delivery note and list of
accessories in Section
structions in Section
5.2). In the case of transport damage, see in-
5.3.1 "Warranty".
2.1.3 Location
This instrument was developed for internal laboratory use; it should not
be used in explosion-endangered locations.
Place the instrument on a suitable vibration-free laboratory bench, protected as much as possible from corrosive atmospheres and contact
with chemicals. Choose a location where the temperature is usually between +5°C and +45°C. The instrument should be protected against
excessive variations in temperature and direct sunlight.
2.2 Mains connection
Mains cable
The instrument is supplied with one of three mains cables
• 6.2122.020 with plug SEV 12 (Switzerland, …)
• 6.2122.040 with plug CEE(7), VII (Germany, …)
• 6.2122.070 with plug NEMA 5-15 (USA, …)
which are three-cored and fitted with a plug with a grounding pin. If a
different plug has to be fitted, the yellow/green lead (IEC standard)
must be connected to protective ground (protection class 1).
Any break in the grounding inside or outside the instrument can make
it a hazard!
Mains connection
Plug the mains cable into mains connection plug
Synthesizer.
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
16 of the 845 Eluent
9
2 Installation
2.3 Connection to PC
The 845 Eluent Synthesizer is controlled by the «Mix Control» PC software (see Section
System requirements
• Processor: Pentium III
• Main memory: 256 MB RAM
• Free memory space: 100 MB for program files
• CD-ROM drive
• Connection: one free USB slot (type A)
Microsoft Windows
must be used as the operating system.
Installation
To connect the instrument and install the software proceed as follows:
3).
TM
2000 or WindowsTM XP or WindowsTM Vista
1 Installing the «Mix Control» program
• Insert installation CD in CD disk drive.
• If autostart has not
<Start> and Run. Search for the Setup.exe file on the in-
stallation CD and click on
• Click on "
Mix Control" and follow the instructions of the
been activated for the CD drive: select
<OK>.
setup program.
2 Connection 845 - PC
• Connect the 845 Eluent Synthesizer (socket "Controller" 12)
to a USB connection (type A) of your computer with the
6.2151.000 Cable (see the instruction manual for your
computer).
• A system test will automatically be carried out on the 845
Eluent Synthesizer.
• The LED "Power"
1 lights up when the system test has been
completed and the instrument is ready for use.
The plug for connection of the 845 Eluent Synthesizer is fitted with a
"pull-out protection device" which prevents the cable from being
pulled out accidentally. When you wish to insert or remove the plug
you must first pull back the outer plug sleeves (marked with arrows).
You can extend the connection with a commercially available USB extension cable (type A/m – type A/f). The length of the connection
should not exceed 5 m. If you require a longer connection then you
must use a USB signal amplifier. Up to five USB signal amplifiers can
be connected in series; this allows a maximum extension of 25 m.
10
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
2.4 Dosino(s)/Stirrer connections
3 Start the «Mix Control» program
• Double click on the icon of the «Mix Control» software.
• The device is recognized automatically.
2.4 Dosino(s)/Stirrer connections
Dosino(s) and stirrer are part of the instrument. The instrument is supplied with them already connected up; these connections should not be
altered.
Correct connection:
Cable markings Connection markings
Dosino 1
Dosino 2
Stirrer
2.5 Tubing connections
The drainage tubing (Section 2.5.2) and the component inlets and outlets (Sections
2.5.3/2.5.4) must be connected.
MSB1
Dosino 1
MSB2
Dosino 2
Stirrer
MSB3
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
11
2 Installation
2.5.1 Instrument with tubing connections
20
7
4
21
22
23
24
6
5
Figure 4: Tubing connections
4 Dosino 1 outlet
Outlet for components from Dosino 1.
5 Connections for components
Connections for components 1 - 5.
7 Dosino 2 outlet
Outlet for components from Dosino 2
(only for version 2.845.0020).
FEP tubing connection
20
6.1838.000 Tubing connections for
6 Recesses for magnetic labels
For identifying components 1 - 5.
12
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
connecting "Dosino 1 outlet"
ino 2" outlet
7 to the bottle adapter 21.
4 / "Dos-
2.5 Tubing connections
Bottle adapter
21
6.1602.180 Bottle adapter as connection piece between eluent bottle
and tubing connection
Eluent bottle
22
6.1608.070 Clear glass bottle for the
mixed eluent.
20.
22
23
24
2.5.2 Connecting the drainage tubing
Connect 6.1816.020 Silicone tubing to connection 18 on the rear panel
of the 845 Eluent Synthesizer and place the other end in a waste bottle.
2.5.3 Connections to bottle adapter
The tubing should not dip into the produced mixture. Make sure that
the FEP- Tubing Connection 6.1838.000
into the bottle.
2.845.0010:
Connect 6.1838.000 FEP Tubing connection
Bottle adapter and to Dosino outlet
the Eluent bottle 6.1608.070.
Pressure screw
6.2744.170 Pressure screw for connecting component capillary
component connection
PTFE capillary
6.1803.130 Capillary for connecting the
components.
20
does not loom too deep
20 to the 6.1602.180 21
4. Then, attach the Bottle adapter to
24 to
5.
2.845.0020:
Connect two 6.1838.000 FEP Tubing connections
21 Bottle adapter and to Dosino outlets 4 and 7. Then, attach the Bottle
adapter to the Eluent bottle 6.1608.070.
2.5.4 Connecting the components
The main component (e.g. water) must be connected to the component connection with the number 1 (see Section
component is used (at the end of the mixing process) to make the
volume up to the total volume given in the method (see Section
3.3).
Proceed as follows to connect the various components:
1 Connect capillary to 845
Connect 6.1803.130 PTFE capillary 24 with 6.2744.170 Pressure
screw 23 to component connection 5.
20 to the 6.1602.180
3.4.2). The main
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
13
2 Installation
2 Connect capillary to the components
Components from a bottle:
• Insert 6.1803.130 PTFE tubing
ment into the component bottle (e.g. the combination
6.1602.160 bottle attachment GL 45 and 6.1608.030 bottle;
see Optional accessories section 5.2.4).
Components from an MPak (from Chata Biosystems, see section
5.2.5):
• Push the 6.1808.200 MPak coupling onto 6.1803.130 PTFE
tubing 24 by loosening the screw cap from the coupling
and pushing the smaller opening over the tubing. Then
screw the coupling into the cap:
24 through the bottle attach-
• Click the 6.1808.200 MPak coupling onto the lower outlet of
the MPak.
2.6 pH Controller
The pH of the prepared mixture can, if necessary, be adjusted automatically by using the (optional) extension kit. If the pH is to be adjusted
from one side then one pH Controller is required, adjustment from both
sides (without changing the titrant solution) requires two pH Controllers.
We recommend the use of 702 Titrinos as pH Controllers (for supply
package see Section
package for required accessories see Section
2.6.1 Tubing connection for pH Controller
If the pH is to be adjusted automatically after the mixing process then
adaptations are required to the tubing connections to the 845 Eluent
Synthesizer.
5.2.3). Other Titrinos can also be used (supply
5.2.3).
1 Replace bottle adapter 21
6.1602.180 Bottle adapter 21 must be replaced by 6.1602.190
Eluent bottle head.
• Screw off the 6.1838.000 FEP tubing connection(s)
6.1602.180 Bottle adapter 21.
• Screw the 6.1838.000 FEP tubing connection(s)
thread(s) of 6.1602.190 Eluent bottle head.
14
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
20 from
20 to M6
2.6 pH Controller
2 Install tubing connection to pH Controller
6.1838.010 FEP tubing connection should be used to make
the tubing connection to the pH Controller.
• Insert the black O-ring belonging to the 6.1838.010 FEP
tubing connection into an M6 thread of 6.1602.190 Eluent
bottle head.
• Then push the tip of the 6.1838.010 FEP tubing connection
through the O-Ring and M6 thread.
• Attach the 6.2726.090 Antidiffusion micro-valve to the tip of
the 6.1838.010 FEP tubing connection.
• Connect the other end of the 6.1838.010 FEP tubing connection to the pH Controller (see Instructions for Use –
Exchange Unit).
3 Screw on bottle adapter
• Now screw 6.1602.190 Eluent bottle head onto eluent bottle
22.
806
4 Insert the Aquatrode in the Eluent bottle head
• Insert 6.0257.020 Aquatrode into the large opening of the
6.1602.190 Eluent bottle head. Watch out, that the Aquatrode looms deep enough into the mixture when the pH is
measured.
2.6.2 pH Controller cable connection
The methods at the pH Controller are started via a remote signal from
the 845 Eluent Synthesizer (see Section
ler to the 845 Eluent Synthesizer as follows:
1 Connect Remote Box
• Connect the 6.2148.010 Remote Box to connection "Remote Box / MSB4"
2 Connect pH Controller
• Connect 6.2141.210 Cable to the remote output of
6.2148.010 Remote Box.
• Connect the end of the 6.2141.210 Cable marked A
remote input of the pH Controller.
• Connect the end of the 6.2141.210 Cable marked B
remote input of the second pH Controller.
The Metrohm 702 Titrino can be used with the SET method to set the
pH to a defined value (see the Instructions for Use of the corresponding Titrino).
3.3.3). Connect the pH Control-
15.
to the
to the
The method for the pH-adjustment and the corresponding parameters
have to be defined on the pH-Controller. The pH-adjustment is started
by a remote signal sent from the 845 Eluent Synthesizer to the pHController. For the installation and operation of the pH Controllers
please refer to the corresponding Instructions for Use.
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
15
3 «Mix Control» software
3 «Mix Control» software
The 845 Eluent Synthesizer is controlled with the «Mix Control» PC
software provided. This section explains the ideas behind the software
and how to use it. For further information please consult the online help
of the software.
3.1 Introduction to the software
3.1.1 The principle of «Mix Control»
What and how much is to be mixed in which ratio is defined via Depot
(see section
Components (see section
specified. The Method is the recipe for preparing the mixture.
3.1.2 Procedure after the first start of «Mix Control»
3.4.1) and Method (see section 3.3). In the Depot the
3.4.2) connected to the instrument are
1. Define the Users (see section 3.4.5).
2. Draw up and save the configuration for the Depot (see section
3.4.1).
3. Draw up and save a Method (see section
After carrying out these steps you can already start mixing processes
(with
In the next step you should define the settings for Directories, Re-
ports, Rinsing and GLP.
).
3.2 Main Window
3.2.1 Menus
Menu File
New... Method /
For creating and configuring a new Method. The parameters of the Method are set to the default values.
3.3).
New... Depot
For creating and configuring a new Depot. The DEPOT
window opens.
Save Method /
For saving the current Method under a new name.
Save Method as...
For saving the current Method under a new name.
16
845 Eluent Synthesizer / Instructions for Use 8.845. 8001EN
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