844 UV/VIS Compact IC
Manual
8.844.1053
Metrohm AG
CH-9101 Herisau
Switzerland
Phone +41 71 353 85 85
Fax +41 71 353 89 01
info@metrohm.com
www.metrohm.com
844 UV/VIS Compact IC
Manual
8.844.1053 03.2008 chs
Teachware
Metrohm AG
CH-9101 Herisau
teachware@metrohm.com
This documentation is protected by copyright. All rights reserved.
Although all the information given in this documentation has been
checked with great care, errors cannot be entirely excluded. Should you
notice any mistakes please send us your comments using the address
given above.
Documentation in additional languages can be found on
http://products.metrohm.com under Literature/Technical documenta-
tion.
Table of contents
Table of contents
1 Introduction ................................................................ 1
1.1 Instrument description ................................................................................1
1.2 Parts and controls .......................................................................................3
1.2.1 Front.................................................................................................................... 3
1.2.2 Rear panel .......................................................................................................... 5
1.2.3 Interior PEEK versions ........................................................................................ 7
1.2.4 Interior Steel versions .........................................................................................9
1.3 Information on the Instructions for Use ...................................................11
1.3.1 Organization .....................................................................................................11
1.3.2 Notation and pictograms.................................................................................. 12
1.4 Safety notes ...............................................................................................13
1.4.1 Electrical safety................................................................................................. 13
1.4.2 General precautionary rules ............................................................................. 13
1.4.3 Recycling and disposal .................................................................................... 13
2 Installation ............................................................... 14
2.1 Overview ....................................................................................................14
2.1.1 Flow chart ......................................................................................................... 14
2.1.2 Connections in the 844 UV/VIS Compact IC.................................................... 15
2.2 Setting up the instrument..........................................................................20
2.2.1 Packaging.........................................................................................................20
2.2.2 Check................................................................................................................ 20
2.2.3 Location ............................................................................................................ 21
2.3 Attaching the accessories.........................................................................21
2.3.1 Connecting the syringe and aspirating tubing................................................. 21
2.3.2 Connecting the drainage tubing for the inner compartment ........................... 21
2.3.3 Connecting PEEK capillaries............................................................................ 21
2.3.4 Connecting Steel capillaries.............................................................................22
2.3.5 Filter unit PEEK ................................................................................................. 23
2.3.6 Filter unit Steel .................................................................................................. 24
2.4 Mains connection ......................................................................................25
2.4.1 Setting the mains voltage................................................................................. 25
2.4.2 Fuses ................................................................................................................ 26
2.4.3 Mains cable and mains connection ................................................................. 26
2.4.4 On/off switching of the instrument ...................................................................27
2.5 Connection to PC ......................................................................................27
2.5.1 Connecting cable .............................................................................................27
2.5.2 Software installation.......................................................................................... 27
2.5.3 First Login ......................................................................................................... 28
2.5.4 Create a system ............................................................................................... 28
2.6 High-pressure pump .................................................................................29
2.6.1 Removing the transport security screws.......................................................... 29
2.6.2 Installing the pulsation dampener.................................................................... 29
2.6.3 Connecting the eluent bottle ............................................................................ 31
2.6.4 Deaerating the pump and rinsing the pulsation dampener............................. 32
2.7 Post column reactor ..................................................................................34
2.7.1 Preparing the peristaltic pump......................................................................... 34
2.7.2 Installation of the post column reactor............................................................. 36
2.7.3 Post column reactor tubing connections ......................................................... 37
2.7.4 Reagent bottle connection ............................................................................... 38
2.8 Columns.....................................................................................................39
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
2.8.1 Precolumns ...................................................................................................... 39
2.8.2 General information on separating columns ................................................... 40
2.8.3 Connecting the separating column (installation without column heating) ...... 41
2.8.4 Connecting the separating column (installation with column heating) ........... 43
2.9 Detector......................................................................................................48
2.9.1 Installing the flow-through cell ......................................................................... 48
2.9.2 Connect the lamps........................................................................................... 49
2.9.3 Flow-through cell tubing connections.............................................................. 49
2.10 Putting into operation................................................................................50
2.10.1 Putting into operation without post column reactor......................................... 50
2.10.2 Putting into operation with post column reactor.............................................. 52
2.11 Connection of external devices ................................................................55
2.11.1 Connection of the 838 Advanced Sample Processor ..................................... 55
2.11.2 Connection of the 813 Compact Autosampler ................................................ 57
3 Operation .................................................................. 59
3.1 «IC Net» - Terms.........................................................................................59
3.2 Measuring operation .................................................................................59
3.2.1 Opening a system ............................................................................................ 59
3.2.2 Defining measuring channels .......................................................................... 60
3.2.3 Connect a system ............................................................................................ 61
3.2.4 Instrument icon................................................................................................. 62
3.2.5 Hardware start/stop and baseline recording ................................................... 62
3.2.6 Determination start/stop................................................................................... 62
3.3 Settings ......................................................................................................63
3.3.1 Instrument control for connected system ........................................................ 63
3.3.2 Hardware settings ............................................................................................ 70
3.3.3 Spectrum window............................................................................................. 74
4 Notes - Maintenance - Faults ................................... 77
4.1 Practical notes on ion chromatography ...................................................77
4.1.1 Separating columns ......................................................................................... 77
4.1.2 High-pressure pump ........................................................................................ 78
4.1.3 Eluents.............................................................................................................. 78
4.1.4 Peristaltic pump................................................................................................ 79
4.1.5 Connections ..................................................................................................... 79
4.2 Maintenance and servicing .......................................................................80
4.2.1 General information.......................................................................................... 80
4.2.2 Shutdown ......................................................................................................... 80
4.2.3 Changing separating columns......................................................................... 80
4.2.4 Maintenance work at the pump head .............................................................. 82
4.2.5 Replacing the pump tubing ............................................................................. 86
4.2.6 Replacing the UV lamp .................................................................................... 87
4.3 Faults and malfunctions............................................................................89
4.3.1 Error messages ................................................................................................ 89
4.3.2 Malfunctions and their rectification .................................................................. 89
4.4 Quality Management and validation with Metrohm .................................91
5 Appendix ................................................................... 92
5.1 Technical data............................................................................................92
5.1.1 UV/VIS Detector................................................................................................ 92
5.1.2 Injection valve................................................................................................... 93
5.1.3 High-pressure pump ........................................................................................ 93
5.1.4 Peristaltic pump................................................................................................ 94
5.1.5 Leak detector ................................................................................................... 94
Table of contents
5.1.6 Gas Sensor....................................................................................................... 94
5.1.7 RS232 interface ................................................................................................ 94
5.1.8 Remote interface .............................................................................................. 95
5.1.9 Mains connection ............................................................................................. 95
5.1.10 Analog output ...................................................................................................96
5.1.11 Control input .....................................................................................................96
5.1.12 Safety specifications......................................................................................... 96
5.1.13 Electromagnetic compatibility (EMC)............................................................... 96
5.1.14 Temperature control column heating ............................................................... 97
5.1.15 Ambient temperature........................................................................................ 97
5.1.16 Housing ............................................................................................................ 97
5.2 Standard equipment..................................................................................98
5.2.1 PEEK versions .................................................................................................. 98
5.2.2 Steel versions ................................................................................................. 103
5.3 Optional accessories ..............................................................................107
5.3.1 General accessories....................................................................................... 107
5.3.2 High-pressure pump ...................................................................................... 108
5.3.3 High-pressure pump Steel ............................................................................. 109
5.3.4 Separating columns and precolumns............................................................ 110
5.3.5 Communication .............................................................................................. 111
5.3.6 Lamps............................................................................................................. 111
5.4 Warranty and Conformity........................................................................112
5.4.1 Warranty (guarantee)...................................................................................... 112
5.4.2 Declaration of Conformity............................................................................... 113
5.4.3 Quality Management Principles ..................................................................... 114
5.5 Index.........................................................................................................115
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
List of figures
Figure 1: Front 844 UV/VIS Compact IC................................................................... 3
Figure 2: Rear 844 UV/VIS Compact IC.................................................................... 5
Figure 3: Interior PEEK versions ............................................................................... 7
Figure 4: Interior Steel Versions................................................................................ 9
Figure 5: Connecting diagram for 844 UV/VIS Compact IC 2.844.0010 ................ 15
Figure 6: Connecting diagram for 844 UV/VIS Compact IC 2.844.0020 with
Post column reactor ................................................................................
Figure 7: Connecting diagram for 844 UV/VIS Compact IC 2.844.0030 ................ 17
Figure 8: Connecting diagram for 844 UV/VIS Compact IC 2.844.0110 with
Column heating .......................................................................................
Figure 9: Connecting diagram for 844 UV/VIS Compact IC 2.844.0120 with
Post column reactor and Column heating ..............................................
Figure 10: Connecting diagram for 844 UV/VIS Compact IC 2.844.0130 with
Column heating .......................................................................................
Figure 11: Connectors for Steel capillaries............................................................... 23
Figure 12: 6.2821.120 Filter unit PEEK ..................................................................... 24
Figure 13: 6.2620.180 Filter unit Steel ...................................................................... 24
Figure 14: Setting the mains voltage........................................................................ 26
Figure 15: Connection of the pulsation dampener................................................... 30
Figure 16: Connection of eluent bottle ..................................................................... 32
Figure 17: Installing pump tubings ........................................................................... 35
Figure 18: Attaching the post column reactor .......................................................... 37
Figure 19: Post column reactor ................................................................................ 38
Figure 20: Connection of reagent............................................................................. 39
Figure 21: Column connection without column heating........................................... 42
Figure 22: Column heating (closed) ......................................................................... 43
Figure 23: Diagram showing separating column in column heating........................ 44
Figure 24: Installing the column heating................................................................... 47
Figure 25: Flow-through cell installation................................................................... 48
Figure 26: Components of the pump head .............................................................. 83
Figure 27: Replacement of the piston seal 140 ....................................................... 84
Figure 28: Components of inlet valve 141 and outlet valve 142............................. 86
Figure 29: UV lamp (deuterium) ............................................................................... 88
Figure 30: Lamp connections (at the back of the 844 UV/VIS Compact IC) ............ 88
16
18
19
20
Table of contents
List of numbered parts and controls
1 Door to inner compartment
34 Filter unit PEEK
2 Feedthrough
3 LED "Power"
4 LED "UV"
5 LED "VIS"
6 Connection purge valve
7 Connection for syringe
8 Feedthrough
9 Lamp cooling housing
10 VIS lamp connection
11 UV lamp connection cable
12 UV lamp connection
13 VIS lamp connection cable
14 Analog output
15 Control input
16 USB-B connection
17 USB-A connection
18 RS232 interface
19 Transport security screws
20 Mains switch
21 Mains connection plug
22 Fuse holder
23 Remote interface
24 Connection capillary
25 Connection capillary
26 Magnetic mounting rail for column
heating
27 Pump head
28 Fastening screws
29 Connection capillary
30 Aspirating capillary
31 Connection capillary purge valve
32 Purge valve
33 Connection capillary to syringe
35 Connection capillary
36 Pulsation dampener
37 Leak detector
38 Inlet capillary
39 Drainage tubing connection
40 Mounting pin
41 Aspirating tubing
42 Flow-through cell
43 UV/VIS detector
44 Mounting rail
45 Sample loop
46 Injection valve
47 Column inlet capillary without column
heating
48 Column inlet capillary with column
heating
49 Peristaltic pump
50 Snap-action lever
51 Pump drive
52 Connection capillary steel
53 Connection capillary steel
54 Pump heard steel 6.2824.040
55 Fastening screws steel
56 Connection capillary steel 6.2620.020
57 Purge valve steel
58 Filter unit steel 6.2620.180
59 Connection capillary steel 6.2620.020
60 Injection valve steel
61 UV/VIS detector steel
62 Flow-through cell steel 6.2839.100
63 Sample loop steel 6.2620.110
64 Compression fitting 6.2744.010
65 Compression fitting 6.2744.070
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
66 Capillary
67 Ferrule (6.2620.010)
68 Ferrule (6.2620.270)
69 Pressure screw (6.2620.000)
70 Steel capillary (6.2620.020)
71 Filter screw of filter unit
72 Filter 6.2821.130
73 Filter housing
74 Steel connection capillary 6.2620.020
75 Steel filter unit 6.2620.180
76 Filter screw of filter unit
77 Filter 6.2620.190
78 Filter housing of filter unit
79 Steel connection capillary 6.2620.020
80 Connection to purge valve
81 Connection to injection valve
82 Aspirating tubing
83 Tubing nipple
84 Threaded stopper
85 Bottle attachment
86 Eluent bottle
87 Aspirating filter
88 CO2 absorber
89 Cotton wool
90 SGJ clip
91 Absorber tube
92 Aspirating tubing for reagent
93 PEEK coupling
94 Pump tubing
95 Stopper (orange-yellow)
96 Tubing cartridge
97 Contact pressure lever
98 Snap-action lever
99 PEEK coupling with filter and tubing
security device
102 PCR holder
103 Connection capillary
104 Post column reactor inlet "eluent in"
105 Post column reactor outlet "to detector"
106 Post column reactor inlet "reagent in"
107 Connection capillary
108 Bottle attachment
109 Supply bottle
110 Steel column inlet capillary
6.2620.160
111 Connection capillary 6.1831.110
112 Steel connection capillary 6.2620.160
113 Separating column
114 Column holder 6.2027.0X0
115 Feedthrough
116 Knurled screw
117 Cover of column heating
118 Connection to 844
119 Magnetic holder
120 Model plate with serial number
121 Feedthrough
122 Connection capillary
123 Connection capillary
124 Steel Connection capillary 6.2620.160
125 Holder plate
126 Capillary prewarming
127 IC Column heating
128 Column heater connection
129 Optical bank
130 Flow-through cell outlet "OUT"
131 Flow-through cell inlet "IN"
132 Knurled screws
133 Screw for piston cartridge
134 Zirconium piston
135 Spring retainer
100 Connection capillary
101 Post column reactor
136 Spring
137 Piston cartridge
Table of contents
138 Piston guide sleeve (4.709.4380)
139 Piston guide sleeve (4.709.4370)
140 Piston seal
141 Inlet valve
142 Outlet valve
143 Screw holder
144 Special tool
145 Special tool
146 Valve housing
147 Sealing ring
148 Sleeve
149 Sapphire sleeve
150 Sapphire sphere
151 Ceramic holder for sapphire sphere
152 Seal
153 UV lamp
154 UV lamp attachment ring
155 Opening for UV lamp
156 Opening for VIS lamp
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
VII
1.1 Instrument description
1 Introduction
1.1 Instrument description
The 844 UV/VIS Compact IC is a PC-controlled instrument for
chromatographic analysis that is available in the six following versions:
• 2.844.0010 UV/VIS Compact IC
• 2.844.0020 UV/VIS Compact IC with post column reactor
• 2.844.0030 UV/VIS Compact IC 844: Steel
• 2.844.0110 UV/VIS Compact IC with column heating
• 2.844.0120 UV/VIS Compact IC with post column reactor
and column heating
• 2.844.0130 UV/VIS Compact IC 844 with column heating:
Steel
All the necessary components for chromatography with UV/VIS
detection are contained in the extremely compact housing of the 844
UV/VIS Compact IC:
• Injection valve – for single injections or for use with a sample
changer, e.g. the 838 Advanced Sample Processor.
• High-pressure pump – extremely low pulsation double-piston
pump with a flow range of 0.2 … 2.5 mL/min and a maximum
pressure of 35 MPa (350 bar).
• Pulsation dampener – the pulsation dampener reliably protects
the columns from damage, even with low-level pressure variations
(not necessairy with versions 2.844.0030 and 2.844.0130).
• Column heating – the perfect insulation of the housing creates
thermally stable conditions for the columns (with Versions
2.844.0110, 2.844.0120 and 2.844.0130).
• Columns – whether anion columns, cation columns or separating
columns for organic acids – they all find space in the column
compartment of the 844 UV/VIS Compact IC.
• Post column reactor – in the post column reactor the eluate from
the column and a reagent delivered by a peristaltic pump are
mixed and transported to the detector. In this way ions that cannot
be detected are converted into (optically) detectable species (with
Versions 2.844.0020 and 2.844.0120).
• UV/VIS detector – for the determination of transitional metals with
post column derivation, chromate, bromate, nitrite and nitrate in
strongly polluted waters, as well as various organic ions.
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
The two steel versions 2.844.0030 and 2.844.0130 are used for HPLC
applications. There is an addittional componet of stel versions:.
• Gas-Sensor – the gas-sensor disrupts power supply, whenever
sovent concentration gets too high.
The operation of the 844 UV/VIS Compact IC takes place via a PC
connected to the RS232 interface with the help of the «IC Net» control
and evaluation program. This PC program can be used to create
systems for recording and evaluating chromatograms. Time programs
can also be created in which a large number of instrument functions
can be triggered for each program step. It is also possible to use programmable signals to control external instruments via the remote
interface.
The «IC Net» operating software meets all the requirements you could
place today on a modern integration software: single or multi-point
calibration, internal or external standard, selectable algorithms for nonlinear calibration, various integration modes with integration parameters
and integration events, different methods for peak recognition, peak
editor, free scaling, superimposing several chromatograms, use of
sample tables and batch reprocessing; a powerful and GLP-conform
report generator with output interfaces for monitor, printer and external
databases.
The independent «Autodatabase» PC program supplied can be used to
save and handle results and chromatograms produced by the «IC Net»
program in a database. With «Autodatabase» data can be sorted,
filtered and searched with the help of different criteria. In addition, data
and curves can be printed out according to user-defined report
templates.
The «IC Net» software can be configured and used in order to comply
with the Electronic Records and Signatures Rule, known as 21 CFR
Part 11, established by the U.S. Food and Drug Administration (FDA).
For this purpose the program contains password protection, user administration, electronic signatures, audit trail and administration of methods and results in databases. To use the 21 CFR Part 11 features of
«IC Net» the operating system Windows 2000 or Windows XP with
NTFS file system is required.
1.2 Parts and controls
8
1.2 Parts and controls
In this Section you will find the numbers and designations of the parts
and controls of the 844 UV/VIS Compact IC. The numbering applies
throughout the Instructions for Use, i.e. bold numbers in the text (e.g.
4
) refer to the parts and controls illustrated here.
1.2.1 Front
1
2
3
4
5
6
Figure 1: Front 844 UV/VIS Compact IC
7
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
Door to inner compartment
1
Feedthrough
2
for eluent, sample, waste, reagent...
LED "Power"
3
lights up when the instrument is
switched on.
LED "UV"
4
lights up when the UV lamp is on.
LED "VIS"
5
lights up when the VIS lamp is on.
Connection purge valve
6
Connection for 6.2816.020 syringe
7
for aspiration of the sample
Feedthrough
8
for eluent, sample, waste, reagent...
1.2 Parts and controls
20 19 14
17
1.2.2 Rear panel
15
16
18
9
10
11
12
13
2
21
22
23
Figure 2: Rear 844 UV/VIS Compact IC
2 Feedthrough
for eluent, sample, waste, reagent...
Lamp cooling housing
9
VIS lamp connection
10
for VIS lamp connection cable
UV lamp connection cable
11
13
UV lamp connection
12
for UV lamp connection cable
VIS lamp connection cable
13
Analog output
14
output for analog signal
Control input
15
11
for control by other instruments
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
USB-B connection
16
USB-A connection
17
RS232 interface
18
PC connection
Transport security screws
19
to secure the pump head when the
instrument is transported
Mains switch
20
to switch instrument on and off:
I = ON 0 = OFF
Mains connection plug
21
Mains connection, see Section
Fuse holder
22
Changing the fuses, see Section
Remote interface
23
Remote-I/O lines for connection of
external devices
2.4
2.4
1.2 Parts and controls
44
43
51
1.2.3 Interior PEEK versions
42
24
25
26
27
28
29
26
30
31
32
45
44
46
47/48
49
50
34
33
35
36
37
38
39
40
41
Figure 3: Interior PEEK versions
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
Connection capillary
24
Connection pump head – purge
valve, fixed mounting
Connection capillary
25
in pump head, fixed mounting
Magnetic mounting rail for co-
26
lumn heating
Pump head (6.2824.100)
27
Fastening screws
28
for pump head
Connection capillary
29
27
from purge valve to Filter unit PEEK.
6.1831.010 PEEK capillary,
length L = 13 cm
Aspirating capillary
30
Connection for 6.1834.010 aspirating
tubing
Connection capillary
31
from purge valve to connection
6
Mounting pin
40
for attaching the tubing cartridges
Aspirating tubing
41
for sample;
PTFE-tubing 6.1803.020
Flow-through cell PEEK
42
6.2839.130
UV/VIS detector
43
Mounting rail
44
for 6.2027.0X0 Column holder, and
6.2057.050 PCR holder
Sample loop
45
PEEK sample loop
Injection valve
46
6.1831.110 Column inlet capillary
47
for column connection without column heating.
6.1831.110 PEEK capillary,
length L = 30 cm
Purge valve
32
Connection capillary to syringe
33
(with manual injection)
6.1803.020 PTFE tubing,
length L = 30 cm
Filter unit PEEK (6.2821.120)
34
Connection capillary
35
from Filter unit PEEK to Pulsation
dampener.
6.1831.010 PEEK capillary,
length L = 13 cm
Pulsation dampener (6.2620.150)
36
Leak detector
37
Inlet capillary for injection valve
38
PEEK capillary 6.1831.010,
length L = 24 cm
6.1831.100 Column inlet capillary
48
for column connection with column
heating.
6.1831.100 PEEK capillary,
length L = 1 m
Peristaltic pump
49
for operating the post column reactor
Holding clamp
50
for locking the tubing cartridge into
place
Pump drive
51
roller head with contact rollers
Connection for drainage tubing
39
for draining off escaped liquid
1.2 Parts and controls
61 41
1.2.4 Interior Steel versions
62
52
53
54
55
56
30
33
57
60
63
59
58
Figure 4: Interior Steel Versions
Steel connection capillary
52
Connection steel pump head – steel
Steel connection capillary
53
in pump head, fixed mounting
purge valve, fixed mounting
Steel pump head 6.2824.040
54
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
Steel fastening screws
55
for steel pump head
Steel connection capillary
56
54
Steel capillary 6.2620.020 from steel
purge valve to steel filter unit.
Steel purge valve
57
Steel filter unit 6.2620.180
58
Steel connection capillary
59
Steel capillary 6.2620.020 from Steel
filter unit to steel injection valve.
Steel Injection valve
60
Steel UV/VIS detector
61
Steel flow-through cell 6.2839.100
62
Steel sample loop 6.2620.110
63
1.3 Information on the Instructions for Use
1.3 Information on the Instructions for Use
Please read through these Instructions for Use carefully before you put
the 844 UV/VIS Compact IC into operation. The Instructions for Use
contain information and warnings to which the user must pay attention
in order to assure safe operation of the instrument.
1.3.1 Organization
These Instructions for Use 8.844.1053 for the 844 UV/VIS Compact
IC provide a comprehensive overview of installation, startup procedure,
operation, fault rectification and technical specifications of this
instrument. The Instructions for Use are organized as follows:
Sect.
1 Introduction
General description of instrument, parts and controls and
safety notes
2 Installation
Sect.
Installation of instrument, accessories, external devices
and software
3 Operation
Sect.
Introduction to the operation
4 Notes - Maintenance - Faults
Sect.
Notes on ion chromatography, maintenance, fault
rectification, diagnostic tests, validation
Sect.
5 Appendix
Technical data, standard equipment, options, warranty,
declarations of conformity, index
To find the required information on the instruments, use either the
Table of contents or the Index at the back.
As a supplement to the Instructions for Use, the Metrohm Monograph
8.792.5003 "Practical Ion Chromatography" is also supplied. This
provides an introduction to the theoretical fundamentals and general
information about determinations.
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
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Table of contents
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these
Instructions for Use:
Fill Menu item, parameter or entry
value
in the software
SYSTEM STATE Program window
in the software
<OK> Button
in the software
26
Part or control of 844
Hazard
This symbol draws attention to a
possible danger to life or of injury if
the associated directions are not
followed correctly.
Warning
This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed
correctly.
Caution
This symbol marks important
information. First read the
associated directions before you
continue.
Comment
This symbol marks additional
information and tips.
1.4 Safety notes
1.4 Safety notes
1.4.1 Electrical safety
While electrical safety in the handling of the 844 UV/VIS Compact IC is
assured in the context of the specifications EN / IEC 61010-1
(protection class
should be noted:
• Mains connection
Setting of the mains voltage, checking the mains fuse and the
mains connection must be effected in accordance with the
instructions in Section
• Opening the 844 UV/VIS Compact IC
If the 844 UV/VIS Compact IC is connected to the power supply, the
instrument must not be opened nor must parts be removed from it,
otherwise there is a danger of coming into contact with components
which are live. Therefore always disconnect the instrument from all
voltage sources before you open it and ensure that the mains cable
is disconnected from mains connection
I, degree of protection IP40), the following points
2.4.
21 !
• Protection against static charges
Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 844 UV/VIS Compact IC, you should ground yourself and
any tools you are using by touching a grounded object (e.g. housing
of the instrument or a radiator) to eliminate any static charges which
exist.
1.4.2 General precautionary rules
• Handling of solvents
Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.
1.4.3 Recycling and disposal
This product is covered by European Directive 2002/96/EC, WEEE –
Waste from Electrical and Electronic Equipment.
The correct disposal of your old equipment will help to prevent negative
effects on the environment and public health.
More details about the disposal of your old equipment can be obtained
from your local authorities, from waste disposal companies or from your
local dealer.
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
13
Table of contents
2 Installation
2.1 Overview
2.1.1 Flow chart
The following flow chart provides an overview of all installation work.
You will find more detailed information in the relevant Sections.
Setting up sect. 2.2
Installing accessories sect. 2.3
Mains connection sect. 2.4
Connectting PC sect. 2.5
Connecting column
Connecting high press. pump sect. 2.6
Post column reactor?
No
Precolumn?
No
Column heating?
No
sect. 2.8.3
Connecting of the detector sect. 2.9
Conditioning sect. 2.10
Yes
Yes
Install post column reactor
Yes
Connecting precolumn sect. 2.8.1
Connecting column and
column heating
sect. 2.7
sect. 2.8.4
Connecting external devices sect. 2.11
2.1 Overview
35 36 38 33 41
45
2.1.2 Connections in the 844 UV/VIS Compact IC
The following illustrations show the internal connections in the 844
UV/VIS Compact IC in schematic form. The meanings of the various
numbered components are given in the detailed illustrations and
descriptions in Sections
2.2 – 2.11.
25
24
Injection valve
47
113
IC Column
43
112
UV/VIS
Detector
27
30
29
34
46
32
82
31
85
86
87
Eluent
Sample
Waste
Figure 5: Connecting diagram for 844 UV/VIS Compact IC 2.844.0010
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
15
Table of contents
47
94 93
41
85
86
87
82
27
30
Eluent
32
25
29
31
24
34
36
35
33
Injection
valve
46
Sample
92
108
109
47
IC Column
45
Reagent
113
103
43
107
99
UV/VIS
Detector
Post column
reactor
101
100
Waste
Figure 6: Connecting diagram for 844 UV/VIS Compact IC 2.844.0020 with
Post column reactor
2.1 Overview
63
61
85
Fehl
86
87
82
30
54
53
56
57
52
31
59
58
33
110 113 112
41
Figure 7: Connecting diagram for 844 UV/VIS Compact IC 2.844.0030
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
17
Table of contents
46 45
85
86
87
82
27
30
25
24
34
35
47
48
Injection
valve
127
Column heating
IC Column
113
43
122
UV/VIS
Detector
29
32
41
31
Eluent
36
33
Waste
Sample
Figure 8: Connecting diagram for 844 UV/VIS Compact IC 2.844.0110 with
Column heating