Metrohm 833IC User Manual
833 IC Liquid Handling Unit
CH-9101 Herisau/Switzerland
E-Mail info@metrohm.com
Internet www.metrohm.com
Instructions for Use
8.833.1003
CH-9101 Herisau/Switzerland
E-Mail info@metrohm.com
Internet www.metrohm.com
833 IC Liquid Handling Unit
Instructions for Use
8.833.1003 10.2003 / pkl
Teachware
Metrohm AG
Oberdorfstrasse 68
CH-9101 Herisau
teachware@metrohm.com
1st Edition 2003
These instructions are protected by copyright. All rights reserved.
Although all the information given in these instructions has been checked with great care, errors
cannot be entirely excluded. Should you notice any mistakes please inform the author at the
address given above.
Table of contents
Table of contents
1 Introduction ................................................................ 1
1.1 Instrument description ................................................................................1
1.1.1 833 IC Liquid Handling Pump Unit..................................................................... 1
1.1.2 833 IC Liquid Handling Suppressor Unit ........................................................... 2
1.1.3 833 IC Liquid Handling Sample Preparation Unit.............................................. 3
1.1.4 833 IC Liquid Handling Dialysis Unit.................................................................. 4
1.1.5 833 IC Liquid Handling Ultrafiltration Unit.......................................................... 5
1.2 Parts and controls .......................................................................................6
1.3 Information about these Instructions for Use ............................................8
1.3.1 Arrangement....................................................................................................... 8
1.3.2 Notation and pictograms.................................................................................... 9
1.4 Safety information .....................................................................................10
1.4.1 Electrical safety................................................................................................. 10
1.4.2 General safety rules.......................................................................................... 10
2 Installation ............................................................... 11
2.1 Instrument setup .......................................................................................11
2.1.1 Packaging......................................................................................................... 11
2.1.2 Checks.............................................................................................................. 11
2.1.3 Location ............................................................................................................ 11
2.1.4 Arranging the instruments ................................................................................ 11
2.2 Mains connection ......................................................................................12
2.2.1 Setting the mains voltage................................................................................. 12
2.2.2 Fuses ................................................................................................................ 13
2.2.3 Mains cable ...................................................................................................... 13
2.2.4 Switching the instrument on/off........................................................................ 13
3 Basic instrument ...................................................... 14
3.1 Electrical connection.................................................................................14
3.1.1 Connecting to 761 Compact IC ....................................................................... 14
3.1.2 Connecting to 819 IC Detector......................................................................... 16
3.1.3 Connecting to 830 IC Interface ........................................................................ 18
3.2 Assembly the pump tubing .......................................................................19
3.3 Operation ...................................................................................................23
3.3.1 Switching the instrument on/off........................................................................ 23
3.3.2 Program settings .............................................................................................. 23
4 833 IC Liquid Handling Pump Unit........................... 27
4.1 Connection of the suppressor module ....................................................27
5 833 IC Liquid Handling Suppressor Unit ................. 31
5.1 Connection of the Suppressor module ....................................................31
5.2 Handling the suppressor module .............................................................35
6 833 IC Liquid Handling Sample Preparation Unit ... 36
6.1 Connection to modular IC system for neutralization .............................36
6.1.1 Electrical connections ...................................................................................... 37
6.1.2 Connection of the sample preparation module ............................................... 38
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
I
Table of contents
6.2 Connection to modular IC system for cation separation ........................43
6.2.2 Settings in IC Net.............................................................................................. 47
6.3 Handling the sample preparation module................................................49
7 833 IC Liquid Handling Dialysis Unit .......................50
7.1 Flow diagram for dialysis..........................................................................51
7.2 Electrical connection.................................................................................52
7.2.1 Dialysis without suppression............................................................................ 52
7.2.2 Dialysis with suppression................................................................................. 53
7.3 Settings in IC Net.......................................................................................54
7.3.1 Dialysis without suppression............................................................................ 54
7.3.2 Dialysis with suppression................................................................................. 55
7.4 Assembling the dialysis cell .....................................................................56
7.5 Making the capillary connections.............................................................57
7.5.1 Preparing the 820 IC Separation Center.......................................................... 57
7.5.2 Connection of the dialysis cell ......................................................................... 58
7.6 Optimizing the dialysis..............................................................................63
7.6.1 Determining the rinsing time ............................................................................ 63
7.6.2 Determining the transfer time........................................................................... 63
7.6.3 Determining the dialysis time........................................................................... 64
7.7 Dialysis procedure ....................................................................................66
8 833 IC Liquid Handling Ultrafiltration Unit.............. 68
8.2 Assembling the ultrafiltration cell.............................................................69
8.3 Connect filtration cell ................................................................................70
8.4 Filtration .....................................................................................................74
8.4.1 Selection of possible sample types ................................................................. 74
8.4.2 Filter working life............................................................................................... 74
8.4.3 Filter membrane selection................................................................................ 75
9 Troubleshooting - maintenance............................... 76
9.1 Faults and their remedies .........................................................................76
9.2 Care and maintenance ..............................................................................79
9.2.1 Instrument care ................................................................................................ 79
9.2.2 Maintenance by Metrohm service.................................................................... 79
9.2.3 Shut down ........................................................................................................ 79
9.2.4 Replacing the pump tubing ............................................................................. 80
9.2.5 Changing the filter ............................................................................................ 81
9.2.6 Suppressor module / Sample preparation module ......................................... 82
9.2.7 Dialysis cell....................................................................................................... 88
9.2.8 Ultrafiltration cell ............................................................................................... 90
10 Appendix ................................................................... 91
10.1 Technical data............................................................................................91
10.1.1 Basic instrument: 833 IC Liquid Handling Unit 1.833.0010............................. 91
10.1.2 6.2729.100 Dialysis cell.................................................................................... 94
10.1.3 6.2729.110 Ultrafiltration cell............................................................................ 94
10.2 Standard equipment ..................................................................................95
10.2.1 833 IC Liquid Handling Pump Unit .................................................................. 95
10.2.2 833 IC Liquid Handling Suppressor Unit ......................................................... 96
10.2.3 833 IC Liquid Handling Sample Preparation Unit............................................ 97
10.2.4 833 IC Liquid Handling Dialysis Unit ............................................................... 98
10.2.5 833 IC Liquid Handling Ultrafiltration Unit...................................................... 100
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
II
Table of contents
10.3 Optional accessories ..............................................................................103
10.3.1 Connection cables ......................................................................................... 103
10.3.2 Pumps............................................................................................................. 104
10.3.3 Modules .......................................................................................................... 106
10.4 Validation / GLP .......................................................................................107
10.5 Warranty and Conformity ........................................................................108
10.5.1 Warranty.......................................................................................................... 108
10.5.2 Declaration of Conformity............................................................................... 109
10.5.3 Quality Management Principles ..................................................................... 110
10.6 Index .....................................................................................................111
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
III
Table of contents
List of figures
Fig. 1: Front panel of 833 IC Liquid Handling Unit ............................................... 6
Fig. 2: Rear panelof 833 IC Liquid Handling Unit................................................. 7
Fig. 3: Setting the mains voltage........................................................................ 13
Fig. 4: Connecting the 833 IC Liquid Handling Unit to 761 Compact IC ........... 14
Fig. 5: Connecting the 833 IC Liquid Handling Unit to 819 IC Detector............. 16
Fig. 6: Connecting the 833 IC Liquid Handling Unit to 830 IC Interface ............ 18
Fig. 7: Assembly the pump tubing ..................................................................... 19
Fig. 8: Connections to suppressor module of 820 Separation Center............... 27
Fig. 9: Suppressor module 1.753.0100 .............................................................. 31
Fig. 10: Suppressor module connections for
833 IC Liquid Handling Suppressor Unit................................................. 32
Fig. 11: 1.793.0110 Sample Preparation Module................................................. 36
Fig. 12: Electrical connections for 833 IC Liquid Handling Sample
Preparation Unit and modular IC system for neutralization..................... 37
Fig. 13: Connections at sample preparation module of
the 833 IC Liquid Handling Sample Preparation Unit.............................. 39
Fig. 14: Connection of 833 IC Liquid Handling Sample Preparation Unit
to modular IC system for cation separation ............................................ 44
Fig. 15: 6.2729.100 Dialysis Cell .......................................................................... 50
Fig. 16: Connecting the 833 IC Liquid Handling Dialysis Unit
to a modular dialysis system without suppression ................................. 52
Fig. 17: Connecting the 833 IC Liquid Handling Dialysis Unit
to a modular dialysis system with suppression....................................... 53
Fig. 18: Time program for dialysis without suppression ...................................... 54
Fig. 19: Time program for dialysis with suppression............................................ 55
Fig. 20 Inserting the dialysis cell in the holder..................................................... 57
Fig. 21: Tubing connection system ...................................................................... 58
Fig. 22: 6.2729.110 Ultrafiltration cell ................................................................... 68
Fig. 23 Inserting the ultrafiltration cell in the holder ............................................. 70
Fig. 24: Tubing connection system ...................................................................... 71
Fig. 25: Filter unit PEEK 6.2821.120 ..................................................................... 81
Fig. 26: Actuator assembly................................................................................... 84
Fig. 27: Adjusting the actuator step ..................................................................... 88
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
IV
1.1 Instrument description
1 Introduction
1.1 Instrument description
Within the modular Advanced IC Systems the 833 IC Liquid Handling
Unit is the universal building block for the preliminary preparation and
post-treatment of samples and for transporting auxiliary and rinsing
solutions. It is available in 5 versions that are matched to the most
frequently required applications:
• 2.833.0010 833 IC Liquid Handling Pump Unit
• 2.833.0020 833 IC Liquid Handling Suppressor Unit
• 2.833.0030 833 IC Liquid Handling Sample Preparation Unit
• 2.833.0040 833 IC Liquid Handling Dialysis Unit
• 2.833.0050 833 IC Liquid Handling Ultrafiltration Unit
1.1.1 833 IC Liquid Handling Pump Unit
The 833 IC Liquid Handling Pump Unit corresponds to the basic
instrument. It has a 2-channel peristaltic pump and can be included in a
modular Metrohm IC system via a remote interface and remotely
controlled by the IC Net software.
It can transport two solutions simultaneously and is particularly suitable
for the operation of the suppressor module in the 2.820.0230 IC
Separation Center for supplying regeneration and rinsing solution.
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
1
1 Introduction
1.1.2 833 IC Liquid Handling Suppressor Unit
As well as the basic instrument, the 833 IC Liquid Handling
Suppressor Unit includes a suppressor module connected to the
basic instrument and also remotely controlled by IC Net.
With the suppressor included in the suppressor module analyses by
ion chromatography with chemical suppression can be carried out. The
suppressor consists of a total of 3 suppressor units which are used in
rotation for suppression, regeneration with sulfuric acid and rinsing with
water. In order that each new chromatogram is recorded under
comparable conditions a freshly regenerated suppressor unit is
normally used. Switching takes place automatically.
The 833 IC Liquid Handling Suppressor Unit is particularly suitable
for retrofitting existing IC systems for chemical suppression in a simple
way. For example, this means that the 833 IC Liquid Handling
Suppressor Unit can be used together with two 819 IC Detectors, two
818 IC Pumps and an 820 IC Separation Center without suppressor
(2.820.0220) to realize a complete 2-channel IC system with only a
single Separation Center.
2
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
1.1 Instrument description
1.1.3 833 IC Liquid Handling Sample Preparation Unit
As well as the basic instrument, the 833 IC Liquid Handling Unit
includes a reactor block (sample preparation module) that is
connected to the basic instrument and also remotely controlled by IC
Net.
With the cation exchanger built-into the sample preparation module
such inline sample preparation steps as neutralization and cation
separation can be carried out for analyses by ion chromatography.
The sample preparation module contains a total of three units which are
used in rotation for cation exchange, regenerated with acid and rinsed
with water. In order that each new chromatogram is recorded under
comparable conditions a freshly regenerated cation exchanger is
normally used. Switching takes place either automatically by the IC
system or manually. As sulfate could interfere with the subsequent IC
analysis, the cation exchanger of the sample preparation module
usually uses perchloric acid (HClO
regeneration.
) instead of sulfuric acid (H2SO4) for
4
The 833 IC Liquid Handling Sample Preparation Unit is particularly
suitable for retrofitting a modular IC system for anion analysis for
sample preparation in a simple way. Two typical applications
(neutralization: exchange of e.g. Na
exchange of e.g. heavy metals for H
Instructions for Use. Although the cation exchange technique used is
comparable with the principle of suppression of the 833 IC Liquid
Handling Suppressor Unit, the 833 IC Liquid Handling Sample
Preparation Unit is not suitable for chemical suppression after the
separation of anions by ion chromatography.
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
+
for H+ and cation separation:
+
) are described in detail in the
3
1 Introduction
1.1.4 833 IC Liquid Handling Dialysis Unit
As well as the basic instrument, the IC Liquid Handling Dialysis Unit
833 includes a dialysis cell upstream from the sample injector.
The 833 IC Liquid Handling Dialysis Unit is used for online sample
preparation in ion chromatography and allows the use of automatic
sample dialysis immediately before sample injection. As well as the 2channel peristaltic pump of the basic instrument for transferring the
sample and acceptor solution from the dialysis cell itself, the ions from
the flowing sample solution are enriched in the stationary acceptor
solution and then injected directly into the IC system. As a result of this
special stopped flow technique, for which an application for a patent
has been made by Metrohm, 100% sample concentration can be
achieved in the acceptor solution and in this way it is possible to carry
out calibration with external standards in a very easy manner.
The operation of the 833 IC Liquid Handling Dialysis Unit requires
the use of an 820 IC Separation Center with one injector and an
additional 833 IC Liquid Handling Pump Unit for the sample tramsport.
These systems, which are described in the Instructions for Use, require
the following instruments:
Operation without
2.819.0110 IC Detector
2.820.0210 IC Separation Center
2.818.0110 IC Pump
2.833.0040 IC Liquid Handling
Dialysis Unit
2.833.0010 IC Liquid Handling
Pump Unit
suppressor
Operation with suppressor
2.819.0110 IC Detector
2.820.0230 IC Separation Center
2.818.0110 IC Pump
2.833.0040 IC Liquid Handling
Dialysis Unit
2.833.0010 IC Liquid Handling
Pump Unit (2 x)
4
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
1.1 Instrument description
1.1.5 833 IC Liquid Handling Ultrafiltration Unit
As well as the basic instrument, the IC Liquid Handling Ultrafiltration
Unit 833 includes a filtration cell upstream from the sample injector.
The 833 IC Liquid Handling Ultrafiltration Unit is an instrument for
online sample preparation in ion chromatography that allows the inline
filtration of the sample immediately before it is injected.
The 833 IC Liquid Handling Ultrafiltration Unit is designed for the
filtration of difficult samples that place special demands on the filtration
effects and sample throughput.
Of course, all versions of the 833 IC Liquid Handling Unit can also be
used with any other commercially available HPLC components.
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
5
1 Introduction
1.2 Parts and controls
In this section you will find the numbers and names of the parts and
controls of the 833 IC Liquid Handling Unit. The numbering is valid for
the whole of these Instructions for Use, i.e. bold numbers in the text
(e.g.
9
) refer to the parts and controls shown here.
Fig. 1: Front panel of 833 IC Liquid Handling Unit
Mains lamp
1
Lights up when instrument is switched
on
Holding pin
2
For inserting the tubing cassettes
32 4 5 6
Contact lever
5
For controlling the contact pressure
Holding clip
6
For clicking the tubing cassette in
place
7
Pump drive
3
Roller head with contact rollers
Tubing cassette
4
For 6.1826.0X0 Pump tubing
6
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
Spring lever
7
For releasing the tubing cassette
1.2 Parts and controls
8
9
11
Fig. 2: Rear panelof 833 IC Liquid Handling Unit
Mains switch
8
Switch for switching instrument on/off:
I = ON 0 = OFF
12 13 14
Module
13
Connection for suppressor module
and sample preparation module
10
15
Mains connection
9
Mains connection: see Section 2.2
Serial number
10
Fuse holder
11
Changing the fuses: see Section 2.2.2.
Step adjustment
12
Adjusts the actuator rotor
REMOTE actuator
14
Remote interface for actuator
Connection to 761 Compact IC, 819 IC
Detector or 830 IC Interface
REMOTE pump
15
Remote interface for pump
Connection to 761 Compact IC,
732/819 IC Detector or 762/830 IC
Interface
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
7
1 Introduction
1.3 Information about these Instructions for Use
Please read through these Instructions for Use carefully before you
start to use the 833 IC Liquid Handling Unit. The instructions contain
information and warnings that must be observed by the user in order
to guarantee the safe use of the instrument.
1.3.1 Arrangement
These 8.833.1003 Instructions for Use for the 833 IC Liquid
Handling Unit provide a comprehensive overview of the installation,
start-up, operation and technical specifications of this instrument. They
are arranged in the following way:
Sect. 1 Introduction
General description of the instrument, numbers and
names of the parts and controls, safety information
Sect. 2 Installation
Instrument setup, mains connection
Sect. 3 Basic instrument
Instrument connection, mounting the pump tubing,
control via «IC Net»
Sect. 4 833 IC Liquid Handling Pump Unit
Instrument connection
Sect. 5 833 IC Liquid Handling Suppressor Unit
Instrument connection, suppressor module handling
Sect. 6 833 IC Liquid Handling Sample Preparation Unit
Instrument connection to modular system for cation
separation and modular system for neutralization, sample
preparation module handling
Sect. 7 833 IC Liquid Handling Dialysis Unit
Instrument connection to modular system with manual
sample injection, dialysis procedure, dialysis optimization
Sect. 8 833 IC Liquid Handling Ultrafiltration Unit
Instrument connection, general information about filtration
Sect. 9 Troubleshooting -
Troubleshooting and remedies, care and maintenance
Sect. 10 Appendix
Technical data, standard equipment, options, warranty,
conformity declarations, index
Please use either the Table of Contents or the Index to find any
information you may require.
8
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
1.3 Information about these Instructions for Use
1.3.2 Notation and pictograms
The following notation and pictograms (symbols) are used in these
Instructions for Use:
Pump Menu item, parameter or input
value
in «IC Net» program
SYSTEM STATE Program window
in «IC Net» program
<OK> Button
in «IC Net» program
[ RUN/STOP ] Switch or key
10 Part or control of 833
14 Part or control of 819/820
Danger/Warning
This symbol indicates a possible
risk of death or injury to the user
and possible damage to the
instrument or its components by
electric current.
Danger/Warning
This symbol indicates a possible
risk of death or injury to the user
and possible damage to the
instrument or its components.
Attention
This symbol indicates important
information that you should read
before continuing.
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
Information
This symbol indicates additional
information and tips which may be
of particular use to you.
9
1 Introduction
1.4 Safety information
1.4.1 Electrical safety
Electrical safety when handling the 833 IC Liquid Handling Unit is
guaranteed within the scope of Standard IEC 1010-1 (protection class
1, protection code IP40). The following points must be observed:
• Mains connection
The mains connection must be made in accordance with the
instructions given in Section 2.2.
• Opening the instrument
The housing contains no components which could be set or adjusted
by the user .
When the 833 IC Liquid Handling Unit is connected to the mains
supply the instrument must not be opened, nor should any of its
components be dismantled as otherwise you could come into contact
with current-carrying components. Before opening the instrument
separate it from all current sources and make sure that mains cable
9
has been removed from mains connection socket
!
• Protection against electrostatic charges
Electronic components are sensitive to electrostatic charges and can
be destroyed by a discharge. Before you touch any electronic
components of the 833 IC Liquid Handling Unit you should ground
you and your tools by grasping a grounded object (e.g. the instrument
housing or a radiator) in order to eliminate any electrostatic charges
that may be present.
1.4.2 General safety rules
• Solvent handling
Check the pump tubing and inlet and outlet connections for leaks at
regular intervals. Observe the relevant regulations when handling and
disposing of flammable and/or toxic solutions.
• Regular replacement of pump tubing
Pump tubing is a consumable and must be replaced from time to
time (see Section 9.2.4). Take suitable measures to ensure that any
leak in the pump tubing or connections during unattended and
continuous operation cannot cause any damage (placing the
instrument in a low position, collection trough for escaping liquid).
10
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
2.1 Instrument setup
2 Installation
2.1 Instrument setup
2.1.1 Packaging
The 833 IC Liquid Handling Unit and its separately packed accessories
are supplied in very protective special packaging. These contain
impact-absorbing plastic foam linings contained inside a blue plastic
film; these are molded to fit the individual components. The instrument
itself is contained in a dustproof evacuated polyethylene bag. Please
store all this special packaging; it is the only way in which the safe
transport of the instrument can be guaranteed.
2.1.2 Checks
Please check that the delivery is complete and undamaged
immediately on receipt (compare with delivery note and list of
accessories given in Section 10.2). If transport damage is evident
please refer to the information given in Section 10.5.1 "Warranty".
2.1.3 Location
Place the instrument on a vibration-free bench in a favorable position
for operation and protected from corrosive atmospheres and
contamination by chemicals.
2.1.4 Arranging the instruments
Other IC instruments (e.g. 818, 819, 820) can be stacked on top of the
833 IC Liquid Handling Unit. It is best to place it beside the modular IC
system or at the bottom of the stack.
The 833 IC Liquid Handling Unit should always be at the bottom so
that any leaks which may occur in the pump tubing or connections
cannot cause any great damage by escaping liquids (e.g. acids).
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
11
2 Installation
2.2 Mains connection
Please observe the following rules when connecting the instrument to
the electricity supply. If the instrument is operated with an incorrectly
set mains voltage and/or an incorrect mains fuse then it represents a
fire hazard!
2.2.1 Setting the mains voltage
Before you switch on the 833 Liquid Handling Unit for the first time
please check that the mains voltage set on the instrument (see Fig. 3)
corresponds to your local mains voltage. If this is not
must alter the mains voltage as follows:
1 Pull out mains cable
Remove the mains cable from mains supply connection 9 of the
833 IC Liquid Handling.
the case then you
2 Remove the fuse holder
Use a screwdriver to loosen fuse holder 11 beneath the mains
supply connection and remove it completely.
3 Check the fuse
Carefully remove the built-in fuse for the intended voltage from
the fuse holder and check its specifications (the position of the
fuse in the fuse holder is indicated by the white arrow beside the
voltage range):
100…120 V 0.5 A (slow blow) Metrohm No. U.600.0013
220…240 V 0.25 A (slow blow) Metrohm No. U.600.0010
4 Insert fuse
Exchange the fuse if necessary and replace it in the fuse holder.
5 Insert fuse holder
Depending on the required mains voltage, insert the fuse holder
in the 833 IC Liquid Handling Unit 833 so that the appropriate
voltage range can be read normally and the adjacent white
arrow points to the white bar printed beneath the fuse holder
(see Fig. 3).
12
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
2.2 Mains connection
–
V
V
Fig. 3: Setting the mains voltage
2.2.2 Fuses
220 –240
100
120
8 Mains switch
8
9 Mains
connection
9
11
220 - 240 V
100 - 120 V
-
220
240 V
-
100
120 V
11 Fuse holder
The fuse holder of the 833 IC Liquid Handling Unit contains one of the
two fuses 0.5 A/slow blow for 100…120 V or 0.25 A/slow blow for
220…240 V.
Make sure that the instrument is never operated with a different type of
fuse as otherwise it represents a fire hazard!
Checking and replacing a fuse is described in Section 2.2.1.
2.2.3 Mains cable
The instrument is supplied with one of the following mains cables:
• 6.2122.020 with SEV 12 plug (Switzerland, …)
• 6.2122.040 with CEE(7), VII plug (Germany, …)
• 6.2133.070 with NEMA 5-15 (USA, …)
which has 3 wires and is fitted with a plug with a grounding pin. If a
different plug has to be attached then the yellow/green wire (IEC
standard) must be connection to the grounding pin.
Any break in the grounding inside or outside the instrument will make
it dangerous!
Insert the mains cable plug in mains connection 9 of the 833 IC Liquid
Handling Unit (see Fig. 2).
2.2.4 Switching the instrument on/off
The 833 IC Liquid Handling Unit is switched on and off with mains
switch 8 (see Fig. 2). When the instrument is switched on mains lamp 1
lights up.
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
13
3 Basic instrument
3 Basic instrument
3.1 Electrical connection
Always switch off the 833 IC Liquid Handling Unit and the instrument
to which the Liquid Handling Unit is to be connected before you make
an electrical connection.
The 833 Liquid Handling Unit can be operated and remotely controlled
by a 761 Compact IC, an 819 IC Detector and in a modular system
via the 830 IC Interface.
3.1.1 Connecting to 761 Compact IC
Connect the remote connection of the 761 Compact IC with the
required remote connections 14 and 15 on the 833 IC Liquid Handling
Unit. The peristaltic pump of the Liquid Handling Unit is controlled via
remote connection Remote Pump 15, this must always be connected.
The connection Remote Actuator 14 is only required for the 833 IC
Liquid Handling Suppressor Unit and the 833 IC Liquid Handling
Sample Preparation Unit; it is used to switch the suppressor or
sample preparation module. The particular modules are connected
to the socket Module 13.
761
833
816
Cable
to
Module
761
833
816
6.2143.200
6.2143.210
Fig. 4: Connecting the 833 IC Liquid Handling Unit to 761 Compact IC
14
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
3.1 Electrical connection
The following cables are available for these connections:
• 6.2143.200 Cable for one remote connection via
Remote line 1.
To connect a Liquid Handling Unit with a peristaltic pump, e.g.:
2.833.0010 IC Liquid Handling Pump Unit
2.833.0050 IC Liquid Handling Ultrafiltration Unit
• 6.2143.210 Cable for two remote connections via
Remote lines 1 and 2.
To connect a Liquid Handling Unit with a peristaltic pump and a
suppressor or sample preparation module, e.g.:
2.833.0020 IC Liquid Handling Suppressor Unit
2.833.0030 IC Liquid Handling Sample Preparation Unit
or
To connect two peristaltic pumps, e.g. for dialysis:
2.833.0010 IC Liquid Handling Pump Unit
2.833.0040 IC Liquid Handling Dialysis Unit
• 6.2143.220 Cable for three remote connections via
Remote lines 1, 2 and 4.
To connect up to three Liquid Handling Units, e.g. for dialysis with
suppression:
2x 2.833.0010 IC Liquid Handling Pump Unit
and 1x 2.833.0040 IC Liquid Handling Dialysis Unit
(three peristaltic pumps are switched on and off)
or
Ultrafiltration with suppression
1x 2.833.0020 IC Liquid Handling Suppressor Unit
and 1x 2.833.0050 IC Liquid Handling Ultrafiltration Unit
(two peristaltic pumps are switched on and off
and the sample preparation module is switched
on)
The remote line via which the control of the Liquid Handling Unit takes
place is determined by the wiring of the cable. The active remote line is
printed on the 833-end of the cable. This remote line must be set
accordingly in the control software «761 Compact IC» or «IC Net», see
Section 4.3.7 In the «761 Compact IC» Instructions for Use and Section
6.5.3 in the «IC Net» Instructions for Use.
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
15
3 Basic instrument
3.1.2 Connecting to 819 IC Detector
Connect the remote connection of the 819 IC Detector to the necessary
remote connections 14 and 15 on the 833 IC Liquid Handling Unit. The
peristaltic pump of the Liquid Handling Unit is controlled via remote
connection Remote Pump 15, this must always be connected. The
connection Remote Actuator 14 is only required for the 833 IC Liquid
Handling Suppressor Unit and the 833 IC Liquid Handling Sample
Preparation Unit; it is used to switch the suppressor or sample
preparation module. The particular modules are connected to the
socket Module 13.
819 819
833
816
6.2143.200
Cable
to
Module
833
816
6.2143.210
Fig. 5: Connecting the 833 IC Liquid Handling Unit to 819 IC Detector
The following cables are available for these connections:
• 6.2143.200 Cable for one remote connection via
Remote line 1.
To connect a Liquid Handling Unit with a peristaltic pump, e.g.:
2.833.0010 IC Liquid Handling Pump Unit
2.833.0050 IC Liquid Handling Ultrafiltration Unit
• 6.2143.210 Cable for two remote connections via
Remote lines 1 and 2.
To connect a Liquid Handling Unit with a peristaltic pump and a
suppressor or sample preparation module, e.g.:
2.833.0020 IC Liquid Handling Suppressor Unit
2.833.0030 IC Liquid Handling Sample Preparation Unit
or
To connect two peristaltic pumps, e.g. for dialysis:
2.833.0010 IC Liquid Handling Pump Unit
2.833.0040 IC Liquid Handling Dialysis Unit
16
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
3.1 Electrical connection
• 6.2143.220 Cable for three remote connections via
Remote lines 1, 2 and 4.
To connect up to three Liquid Handling Units, e.g. for dialysis with
suppression:
2x 2.833.0010 IC Liquid Handling Pump Unit
and 1x 2.833.0040 IC Liquid Handling Dialysis Unit
(three peristaltic pumps are switched on and off)
or
Ultrafiltration with suppression
1x 2.833.0020 IC Liquid Handling Suppressor Unit
and 1x 2.833.0050 IC Liquid Handling Ultrafiltration Unit
(two peristaltic pumps are switched on and off
and the sample preparation module is switched
on)
The remote line via which the control of the Liquid Handling Unit takes
place is determined by the wiring of the cable. The active remote line is
printed on the 833-end of the cable. This remote line must be set
accordingly in the control software «IC Net», see Section 6.4.3 of the
«IC Net» Instructions for Use.
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
17
3 Basic instrument
3.1.3 Connecting to 830 IC Interface
Connect one of the event lines of the 830 IC Interface with one of the
required remote connections 14 and 15 on the 833 IC Liquid Handling
Unit. The peristaltic pump of the Liquid Handling Unit is controlled via
remote connection Remote Pump 15, this must always be connected.
The connection Remote Actuator 14 is only required for the 833 IC
Liquid Handling Suppressor Unit and the 833 IC Liquid Handling
Sample Preparation Unit; it is used to switch the suppressor or
sample preparation module. The particular modules are connected
to the socket Module 13.
830
833
816
Cable
to
Module
6.2128.180
830
833
816
6.2128.180
6.2128.180
Fig. 6: Connecting the 833 IC Liquid Handling Unit to 830 IC Interface
For each remote connection one 6.2128.180 Cable is required. At the
830 IC Interface 2 × 7 remote connections can be assembled. Which
event lines are used for this does not matter; when a system is set up
the assignment must only be given correctly in the «IC Net» software
when creating a system, see Section 6.1 of the «IC Net» Instructions for
Use.
18
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
3.2 Assembly the pump tubing
3.2 Assembly the pump tubing
Pump tubing is a consumable whose working life depends on the
contact pressure. This is why when the pump is switched off for a long
time, the tubing cassette should be lifted by fully loosening spring
lever
7
on the right-hand side (in this way the correctly set optimal
contact pressure is retained).
The 6.1826.0X0 Pump tubing consists of PVC or PP and must
therefore not be rinsed with solvents that contain acetone. In this case
you should either use different pump tubing or use a different pump
for rinsing.
18 17 16 4 519
Tubing cassette
4
Contact lever
5
Spring lever
7
6.1803.0X0 Aspiration
16
tubeing/capillary
PTFE tubing/capillary, depends on
version, see Section 10.2 Standard
equipment
20 22 21 20 17
Fig. 7: Assembly the pump tubing
7
Stopper
20
The color of the stopper indicates the
material and dimensions of the pump
tubing, see Tab. 1.
6.2744.160 PEEK Coupling
21
with tubing security device
6.2821.120 PEEK Filter unit
22
not contained in all versions, see
Section 10.2 Standard equipment
17 1716
23
6.2744.010 PEEK compression
17
fitting
6.2744.034 PEEK coupling
18
6.1826.0X0 Pump tubing
19
various tubing is available, depending
on the application, see Tab. 1.
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
Discharge capillary
23
delivery side for connection to:
suppressor module
sample preparation module
dialysis cell
ultrafiltration cell
19
3 Basic instrument
1 Remove tubing cassettes
• Loosen the two tubing cassettes 4 by pressing in spring lever
7 of holding clip 6 and unhinge them from holding pin 2 of
the 833 IC Liquid Handling Unit (see Fig. 1).
2 Insert pump tubing
• Press contact lever 5 on the two tubing cassettes down as far
it will go.
• Insert pump tubing into each tubing cassette as shown in
Fig. 7.
Stopper must click into position in the corresponding holder
on the left-hand side of the tubing cassette.
3 Attach tubing cassettes
• Hinge the tubing cassettes on holding pin and press down
on the right –hand side until spring lever clicks into position
in holding clip 6. Take care that the pump tubing does not
kink.
4 Attach coupling to pump tubing
• Attach PEEK coupling 18 to the aspiration end of pump
tubing 19 for both channels (see Fig. 7).
•
Mount PEEK coupling 21 with the tubing security device
(6.2744.160) to the delivery end of pump tubing 19 for both
channels.
This is done by dismantling the tubing security device and
first pushing the sleeve nut and the compression piece onto
the tubing.
Attach the tubing to the PEEK coupling and screw the sleeve
nut onto the coupling in order to secure the tubing.
5 Mounting of filter unit
• If contained in the standard equipment (see Section 10.2
Standard equipment), screw 6.2821.120 PEEK filter unit 22
onto PEEK coupling 21 (6.2744.160) by using two PEEK
compression fittings 17 (6.2744.010) and a piece of
aspiration tubing/capillary 16 (6.1803.0X0) at the delivery end
of pump tubing 19.
Setting the contact pressure correctly:
Press contact lever 5 until the solutions are just aspirated. Then press
the contact lever up by 1 further click to achieve the optimum contact
pressure.
20
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
3.2 Assembly the pump tubing
In addition to the correct contact pressure, the delivery rate of the 833
IC Liquid Handling Unit depends primarily on the inner diameter of
pump tubing 19. Different pump tubing can be used depending on the
application.
The following table provides information about the properties and use
of the available pump tubing.
Tab. 1: Using 6.1826.0X0 Pump tubing
Used with Order no. Description
2.833.0030 IC Liquid
Handling Sample
Preparation Unit:
both channels
6.1826.010 Pump tubing
PVC (Tygon
ST) with 2 permanently
mounted white-white stoppers;
i.d. = 1.02 mm ± 0.05 mm,
Delivery rate
1.41 mL/min (20 min
1.69 mL/min (24 min
optional 6.1826.020 Pump tubing
PVC (Tygon
ST) with 2 permanently
mounted blue-blue stoppers;
i.d. = 1.65 mm ± 0.05 mm,
Delivery rate
3.75 mL/min (20 min
4.50 mL/min (24 min
2.833.0040 IC Liquid
Handling Dialysis Unit:
channel for supplying
acceptor solution
2.833.0050 IC Liquid
Handling Ultrafiltration
Unit:
channel for transferring
filtrate to sample loop
2.833.0040 IC Liquid
Handling Dialysis Unit:
channel for supplying
sample
6.1826.030 Pump tubing
PVC (Tygon
ST) with 2 permanently
mounted orange-yellow stoppers;
i.d. = 0.51 mm ± 0.05 mm,
Delivery rate
0.40 mL/min (20 min
0.48 mL/min (24 min
6.1826.040 Pump tubing
PVC (Tygon
mounted black-black stoppers;
i.d. = 0.76 mm ± 0.05 mm,
Delivery rate
ST) with 2 permanently
0.75 mL/min (20 min
0.90 mL/min (24 min
150
-1
)
-1
)
-1
)
-1
)
-1
)
-1
)
-1
)
-1
)
400
150
400
150
400
150
400
2.833.0010 IC Liquid
Handling Pump Unit,
2.833.0020 IC Liquid
Handling Suppressor
Unit:
both channels
6.1826.050 Pump tubing
PVC (Tygon
mounted white-yellow stoppers;
i.d. = 0.57 mm ± 0.05 mm,
Delivery rate
0.52 mL/min (24 min
optional 6.1826.060 Pump tubing
PP (PharMed
mounted orange-yellow stoppers;
i.d. = 0.51 mm ± 0.05 mm,
Delivery rate
0.56 mL/min (24 min
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
ST) with 2 permanently
0.43 mL/min (20 min
) with 2 permanently
0.47 mL/min (20 min
150
-1
)
-1
)
-1
)
-1
)
400
150
400
21
3 Basic instrument
2.833.0050 IC Liquid
Handling Ultrafiltration
Unit:
channel for transferring
sample to ultrafiltration cell
6.1826.070 Pump tubing
PVC (Tygon
ST) with 2 permanently
mounted yellow-yellow stoppers;
i.d. = 1.42 mm ± 0.05 mm,
Delivery rate
2.55 mL/min (20 min
3.06 mL/min (24 min
optional 6.1826.110 Pump tubing long-life
PVC (Tygon
mounted orange-yellow stoppers;
i.d. = 0.51 mm ± 0.0102 mm,
Delivery rate
LFL) with 2 permanently
0.40 mL/min (20 min
0.48 mL/min (24 min
optional 6.1826.120 Pump tubing long-life
PVC (Tygon
mounted orange-white stoppers;
i.d. = 0.59 mm ± 0.05 mm,
Delivery rate
LFL) with 2 permanently
0.44 mL/min (20 min
0.53 mL/min (24 min
optional 6.1826.130 Pump tubing long-life
PVC (Tygon
mounted white-white stoppers;
i.d. = 1.02 mm ± 0.0127 mm,
Delivery rate
LFL) with 2 permanently
1.41 mL/min (20 min
1.69 mL/min (24 min
150
-1
)
-1
)
-1
)
-1
)
-1
)
-1
)
-1
)
-1
)
400
150
400
150
400
150
400
22
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
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