Metrohm 818IC User Manual

Download

818 IC Pump

Manual

8.818.1023

Metrohm AG CH-9101 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com

818 IC Pump

5.818.0110 Program

Manual

8.818.1023 11.2005 chs

Teachware Metrohm AG CH-9101 Herisau teachware@metrohm.com

Although all the information given in this documentation has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please send us your comments using the address given above.

Documentation in additional languages can be found on

http://products.metrohm.com under Literature/Technical documenta-

tion.

Table of contents

1 Introduction.................................................... 1

1.1 Instrument description ............................................................. 1

1.2 Parts and controls .................................................................... 2

1.3 Information about the Instructions for Use ............................. 4

1.3.1 Organization ..................................................................................4

1.3.2 Notation and pictograms ..............................................................5

1.4 Safety notes ..............................................................................6

1.4.1 Electrical safety..............................................................................6

1.4.2 General safety rules.......................................................................6

2 Installation ..................................................... 7

2.1 Setting up the instrument ......................................................... 7

2.1.1 Packaging...................................................................................... 7

2.1.2 Check.............................................................................................7

2.1.3 Location ......................................................................................... 7

2.1.4 Arrangement of the instruments....................................................7

2.2 Mounting the pump head ......................................................... 8

2.3 Attaching the accessories........................................................ 9

2.3.1 Capillaries ...................................................................................... 9

2.3.2 Pulsation dampener ......................................................................9

2.3.3 Filter unit PEEK............................................................................10

2.4 Connecting the tubing ............................................................ 11

2.4.1 Connection to injection valve ......................................................11

2.4.2 Connection to eluent container ...................................................12

2.5 Electrical connection .............................................................. 13

2.5.1 Connection to 830 IC Interface ...................................................13

2.5.2 Connection to PC ........................................................................14

2.6 Mains connection.................................................................... 15

2.6.1 Setting the mains voltage............................................................15

2.6.2 Fuses ...........................................................................................16

2.6.3 Mains cable .................................................................................16

2.6.4 On/off switching of the instrument ..............................................16

2.7 Software installation ............................................................... 17

2.8 Deaerating the pump .............................................................. 17

3 Operation...................................................... 18

3.1 Switch instrument on/off ........................................................ 18

3.2 818 IC Pump icon.................................................................... 18

3.3 Settings in the "818 IC Pump" window................................... 18

4 Notes – Maintenance – Faults...................... 23

4.1 Practical notes ........................................................................ 23

4.1.1 Protection against foreign particles ............................................23

4.1.2 Pulsation dampener ....................................................................23

4.1.3 Eluents......................................................................................... 23

4.2 Maintenance and servicing ....................................................24

4.2.1 General information.....................................................................24

4.2.2 Maintenance work at the pump head .........................................25

4.3 Faults and malfunctions ......................................................... 30

818 IC Pump / 8.818.1023 Instructions for Use

Table of contents

5 Interfaces ..................................................... 31

5.1 RS 232 interface...................................................................... 31

5.1.1 Data transmission protocol......................................................... 31

5.1.2 Pin assignment............................................................................ 32

5.2 Control interface ..................................................................... 33

5.2.1 Functions..................................................................................... 33

5.2.2 Control inputs and outputs .........................................................33

6 Appendix ....................................................... 36

6.1 Technical specifications......................................................... 36

6.2 Scope of delivery .................................................................... 39

6.3 Optional accessories .............................................................. 41

6.4 Validation / GLP ...................................................................... 44

6.5 Warranty and Conformity .......................................................45

6.5.1 Warranty ......................................................................................45

6.5.2 Declaration of Conformity ...........................................................46

6.5.3 Quality Management Principles .................................................. 47

6.6 Index ........................................................................................ 48

List of figures

Fig. 1: Front of 818 IC Pump....................................................................... 2

Fig. 2: Rear of 818 IC Pump ....................................................................... 3

Fig. 3: Connect capillaries .......................................................................... 9

Fig. 4: Filter unit PEEK (6.2821.120) ......................................................... 10

Fig. 5: Connection to injection valve......................................................... 11

Fig. 6: Connection of 818 IC Pump to 830 IC Interface ............................ 13

Fig. 7: Connection of 818 IC Pump to PC ................................................ 14

Fig. 8: Components of the pump head .................................................... 26

Fig. 9: Replacement of the piston seal 34................................................ 26

Fig. 10: Components of inlet valve 35 and outlet valve 36......................... 29

818 IC Pump / 8.818.1023 Instructions for Use

1.1 Instrument description

1 Introduction

1.1 Instrument description

The 818 IC Pump is a serial dual piston pump especially developed for ion chromatography and optimized for use with Metrohm ion chromatographic instruments (e.g. 819 IC Detector and 820 IC Separation Center). The 818 IC Pump operates with minimal residual pulsation and has an excellent flow constancy. All wet parts of the pump are metal free.

The 818 IC Pump is completely remote controlled by the Metohm software «IC Net» (version 2.3 or higher). All functions are fast and easily accessible via software and can be clearly arranged at the monitor. To assure optimum operational reliability, both lower and upper pressure limit values can be set. If such a limit is reached, the pump switches itself off automatically. An additional purge valve allows rapid removal of air from the pump system.

818 IC Pump / 8.818.1023 Instructions for Use

1 Introduction

1.2 Parts and controls

In this section you will find the numbers and designations of the parts and controls of the 818 IC Pump. The numbering applies throughout the instructions for use, i.e. bold numbers in the text (e.g. the parts and controls illustrated here.

1 2 3 4 5

) refer to

Fig. 1: Front of 818 IC Pump

Mains pilot lamp

Lights up when instrument is switched on

Purge valve

Connection capillary

to injection valve of IC system.

Connection capillary

in pump head (intergal)

6 7 8 9

Outlet capillary

of Purge-Ventil.

Connection capillary

between pump head and purge valve.

Aspirating capillary

Pump head 6.2824.100

Fastening screws

for pump head

818 IC Pump / 8.818.1023 Instructions for Use

1.2 Parts and controls

Fig. 2: Rear of 818 IC Pump

Fuse cover

Changing the fuses, see section 2.6.2. Mains switch For switching instrument on/off: I = ON 0 = OFF Mains connector For mains connection see section 2.6.

1110

13 14 15 16 17 18

Recorder output

For recording the pressure, 1 mV = 1 MPa

Switch "Time Constant"

Time constant for the recorder output: HIGH ≈ 1 s; LOW ≈ 20 ms

Mains voltage selector

Voltage selection see section 2.6.1.

Serial number

Control interface

details see section 5.2.

Switch for external control

Selector to choose the interface for remot control of the pump RS 232 / Current Loop

RS 232 interface

Current Loop interface

(Details on request)

818 IC Pump / 8.818.1023 Instructions for Use

1 Introduction

1.3 Information about the Instructions for Use

Please read through these Instructions for Use carefully before operating the 818 IC Pump. The Instructions for Use contain information and warnings to which the user must pay attention in order to assure safe operation of the instrument.

1.3.1 Organization

These 8.818.1023 Instructions for Use for the 818 IC Pump provide a comprehensive overview of the installation, startup procedure, operation, fault rectification and technical specifications of this instrument. The Instructions for Use are organized as follows:

Section 1 Introduction

Description of the instrument, parts and controls, safety notes

Section 2 Installation

Mounting the pump head, electrical connections, tubing connections, mains connection

Section 3 Operation

Operation via «IC Net»

Section 4 Notes – Maintenance – Faults

Practical notes, maintenance, fault rectification

Section 5 Interfaces

Description of the interfaces

Section 6 Appendix

Technical data, standard equipment, options, validation, warranty, declaration of conformity, index

To find the information you require about the instrument please use either the Table of contents or the Index at the back.

818 IC Pump / 8.818.1023 Instructions for Use

1.3 Information about the Instructions for Use

1.3.2 Notation and pictograms

The following notations and pictograms (symbols) are used in these Instructions for Use:

Range Menu item, parameter or entry

value

in «IC Net» program

SYSTEM STATE Program window

in «IC Net» program

<OK> Button

in «IC Net» program

10 Part or control of 818

Danger/Warning

This symbol indicates a possible risk of death or injury to the user and possible damage to the instrument or its components by electricity.

Danger/Warning This symbol indicates a possible risk of death or injury to the user and possible damage to the instrument or its components.

Attention This symbol indicates important information that you should read before continuing.

Information

This symbol indicates additional information and tips which may be of particular use to you.

818 IC Pump / 8.818.1023 Instructions for Use

1 Introduction

1.4 Safety notes

1.4.1 Electrical safety

While electrical safety in the handling of the 818 IC Pump is assured in the context of the specifications IEC 1010-1 (protection class 1, degree of protection IP40), the following points should be noted:

• Mains connection

Set the mains voltage and check the mains fuse and mains connection in accordance with the instructions in section 2.6.

• Opening the instrument

Inside the instrument there are no parts which must be set or adjusted by the user.

If the 818 IC Pump is connected to the power supply, the instrument must not be opened nor must parts be removed from it, otherwise there is a danger of coming into contact with components which are live. Hence, always disconnect the instrument from all voltage sources before you open it and ensure that the mains cable is disconnected from mains connection 10 !

• Protection against static charges

Electronic components are sensitive to static charging and can be destroyed by discharges. Before you touch any of the components inside the 818 IC Pump, you should earth yourself and any tools you are using by touching an earthed object (e.g. housing of the instrument or a radiator) to eliminate any static charges which exist.

1.4.2 General safety rules

• Handling of solvents

Check the pump tubing and all input and output leads periodically for possible leaks. Follow the relevant instructions regarding the handling of flammable and/or toxic solvents and their disposal.

818 IC Pump / 8.818.1023 Instructions for Use

2.1 Setting up the instrument

2 Installation

2.1 Setting up the instrument

2.1.1 Packaging

The 818 IC Pump is supplied together with the separately packed accessories in special packagings containing shock-absorbing foam linings designed to provide excellent protection. The actual instrument is packed in an evacuated polyethylene bag to prevent the ingress of dust. Please store all these special packagings as only they assure transport of the instruments free from damage.

2.1.2 Check

After receipt, immediately check whether the shipment is complete and has arrived without damage (compare with delivery note and list of accessories in section 6.2). In the case of transport damage, see instructions in section 6.5.1 "Warranty".

2.1.3 Location

Position the 818 IC Pump in the laboratory at a location convenient for operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals.

To avoid disturbing temperature influences on the insulated column compartment, the pump and eluent reservoir must be protected against direct sunlight.

2.1.4 Arrangement of the instruments

The 818 IC Pump should always be at the very bottom of a IC system.

The 818 IC Pump should always be placed at the bottom of the instrument stack so that any leaks which may occur in the pump tubing or connections cannot cause damage to the other instruments by leakage liquids.

818 IC Pump / 8.818.1023 Instructions for Use

2 Installation

2.2 Mounting the pump head

1 Remove the transportation lock

To ensure that the pump drive is not damaged during transport a black plastic block is mounted in place of the pump head on the right side of the 818 IC Pump front panel. This transport safeguard block has to be removed after undoing the four screws 9.

To avoid any damage to the pump head, the protective block must be remounted if the pump has to be moved any great distance.

2 Mounting the pump head

The Pump Head 8 (6.2824.100) with integrated piston units is unpacked and fixed in place of the transport safeguard block with the four hexagon screws 9as shown in Fig. 2 .

To ensure that the pump head is not positioned wrongly, the holes at the rear for the clamping bolts have different depths, i.e. 1 clamping bolt is longer than the rest. The deepest hole must naturally accommodate the longest bolt. If this is not the case, the pump will not function properly.

3 Connecting capillary to pump head

The connection capillary 6 fastened to purge valve 2 is screwed onto the pump head 8 as shown in Fig. 2.

The pressure range of the 818 IC Pump with the 6.28.24.100 PEEK

…

pump head isf 0

30.0 MPa (0…300 bar)..

818 IC Pump / 8.818.1023 Instructions for Use

2.3 Attaching the accessories

2.3.1 Capillaries

Some of the connections under high pressure between the 818 IC Pump and the IC system must be set up by the user, see section 2.4. For that the 6.1831.010 PEEK capillary (i.d. = 0.25 mm, o.d. = length = 3 m) supplied in the accessories of the 818 IC Pump can be used.

Capillaries fitted with new connectors must have a perfectly flat cut surface. For PEEK capillaries it is best to use the 6.2621.080 Capillary tubing cutter

/16",

Fig. 3: Connect capillaries

PEEK capillary (6.1831.010)

20 19

Compression fitting (6.2744.010)

For the connection of 6.1831.010 PEEK capillaries or of the 6.1834.000 PTFE aspirating tubing, the supplied 6.2744.010 PEEK Compression fittings are used. It is best to proceed as follows:

1 Mount compression fitting

Slide a compression fitting 20 (6.2744.010) over the end of the capillary 19 to be fastened as shown in Fig. 3.

2 Insert capillary in connection

Push capillary end in the corresponding connection as far as it will go (to avoid dead volume).

3 Tighten compression fitting

Tighten compression fitting 20 by hand (never use tools).

2.3.2 Pulsation dampener

To protect the column material against pressure drops caused by the injector, the use of a pulsation dampener connected between the pump and the injection valve of the 820 IC Separation Center is recommended. The optional 6.2620.150 Pulsation dampener MF is very well suited to this purpose (see section 6.3).

The metal-free 6.2620.150 Pulsation dampener is supplied fully assembled and has two connections for capillaries, for which two

818 IC Pump / 8.818.1023 Instructions for Use

2 Installation

6.2744.010 PEEK compression fittings can be used. The flow direction is arbitrary. The pulsation dampener is positioned in the interior of the 820 IC Separation Center on the base below the injection valve (see

819/820 Instructions for Use).

The pulsation dampener is filled with isopropanol when new. Rinse your IC system carefully after the first installation of a new pulsation dampener.

2.3.3 Filter unit PEEK

The 6.2821.120 Filter unit PEEK 24 supplied (see Fig. 4) serves to avoid contamination of the piston seals by abrasive particles and can be used in the pressure range 0…30 MPa (0…300 bar). The filter unit consists of the housing 21, the filter 22 and the connector 23 to be screwed into the housing 21. For the connection of capillaries 19, PEEK compression fittings 20 (6.2744.010) must be used.

New filter 22 are available as an option with the ordering number

6.2821.110 (10 pieces).

For the connection of the filter unit, please note the flow direction arrow printed on the housing.

The filter unit is filled with isopropanol when new. Rinse your IC system carefully after the first installation of a new filter unit.

20 19 20 232221 19

Fig. 4: Filter unit PEEK (6.2821.120)

PEEK capillary (6.1831.010)

Compression fitting (6.2744.010)

Filter housing

Filter 6.2821.130

Filter connector

818 IC Pump / 8.818.1023 Instructions for Use

2.4 Connecting the tubing

2.4.1 Connection to injection valve

It is recommended to use 6.1831.010 PEEK capillaries, a 6.2620.150 Pulsation dampener (see section 2.3.2), and a Filter unit PEEK (see section 2.3.3) to connect the 818 IC Pump to the injection valve of the 820 IC Separation Center. Proceed as follows:

25 243

Fig. 5: Connection to injection valve

Connection capillary

to 820 IC Separation Center.

PEEK capillary (6.1831.010)

818

Filter unit PEEK (6.2821.120)

Pulsation dampener (6.2620.150)

820

1 Connection to 818 IC Pump

• Cut connection capillary 3 (6.1831.010 PEEK capillary) to the

required length and equip it with compression fittings.

• Attach connection capillary 3 to the upper connection of the

purge valve (see Fig. 1).

2 Connection of filter unit PEEK

• Attach the other end of connection capillary 3 at the filter

housing 21 of the filter unit 24 (see Fig. 4).

• Connect a PEEK capillary 6.1831.010 cut to the required

length and equipped with connectors to the filter connector 23 of the filter unit 24.

3 Installation of the capillary in the IC Separation

Center

4 Connection of the pulsation dampener

5 Connection to the injection valve

818 IC Pump / 8.818.1023 Instructions for Use

• Procedure, see 819/820 Instructions for Use.

• Procedure, see section 2.3.2.

• Procedure, see section 2.3.3.

2 Installation

2.4.2 Connection to eluent container

The 6.1834.000 Aspirating tubing (i.d. = 1.5 mm, o.d. = 2.5 mm, length = 1.2 m) supplied is slid over the aspirating capillary 7. The

6.2821.090 Aspirating filter supplied is screwed onto the other end of the tubing. The end of the tubing with the aspirating filter is then introduced in the eluent container.

Only degassed (with N

, He or vacuum) and microfiltered (0.45 µm

filter) eluents may be used!

It must be ensured that the eluent used is freely miscible with any residual solvent in the pump head (the pump head is filled with isopropanol or methanol/water in the factory). If this is not the case, the pump must first be rinsed with a solvent that is miscible with both the previous and subsequent eluent (e.g. acetone).

If you use the optional available 6.5324.000 Bottle rack, the

6.1834.010 Aspirating tubing (accessory of the bottle rack, length =

2.5 m) must be installed instead of the 6.1834.000 Aspirating tubing. The end of the tubing with the screwed-on 6.2821.090 aspirating filter is then fixed in the eluent vessel according to the instructions on the leaflet supplied with the bottle rack.

818 IC Pump / 8.818.1023 Instructions for Use

2.5 Electrical connection

Switch off both devices before connecting the 818 IC Pump to another instrument.

2.5.1 Connection to 830 IC Interface

The 818 IC Pump is normally integrated into the modular IC system via 830 IC Interface. Connect the RS 232 interface 17 of the 818 IC Pump with a free RS 232 interface at the 830 IC Interface using the 6.2134.080 or 6.2134.090 cable according to the figure below. To ensure proper functioning of the communication between the 830 IC Interface and 818 IC Pump, the sliding switch 16 on the IC pump must be set to "RS 232".

6.2134.100

830

818

6.2134.080/6.2134.090

CURRENT

LOOP

RS 232

Fig. 6: Connection of 818 IC Pump to 830 IC Interface

818 IC Pump / 8.818.1023 Instructions for Use

2 Installation

2.5.2 Connection to PC

The 818 IC Pump can also be connected directly to a PC. Connect the RS 232 interface 17 of the 818 IC Pump with a free COM port at the PC using the 6.2125.110 (for 9 pin COM port connector) or 6.2125.060 cable (for 25 pin COM port connector) according to the figure below. To ensure proper functioning of the communication between the PC and 818 IC Pump, the sliding switch 16 on the IC pump must be set to "RS 232".

818

6.2125.110/6.2125.060

CURRENT

LOOP

RS 232

Fig. 7: Connection of 818 IC Pump to PC

818 IC Pump / 8.818.1023 Instructions for Use

2.6 Mains connection

Follow the instructions below for connecting to the power supply. If the instrument is operated with a mains voltage set wrongly and/or wrong mains fuse, there is a danger of fire!

2.6.1 Setting the mains voltage

Before switching on the 818 IC Pump for the first time, check whether the mains voltage set on the instrument (can be read in the mains voltage selector 11) matches the local mains voltage. If this is not the case, you must switch the mains voltage by pushing the mains voltage selector 11 with a screwdriver:

Position of the mains voltage selector 11:

1 Disconnect mains cable

Disconnect mains cable from mains connection plug 10 of the 818 IC Pump.

2 Remove fuse cover

Using a screwdriver, lever out fuse cover 10 forwards until it opens.

3 Check and change fuses if necessary

Carefully take the fuses installed for the desired mains voltage out of the fuse holder and check its specifications:

100…120 V 0.5 A (slow blow) Metrohm No. U.600.0013 220…240 V 0.25 A (slow blow) Metrohm No. U.600.0010

Change fuse if necessary and reinsert in fuse holder.

230V

115V

230V: 220…240 V ± 10%

115V: 110…120 V ± 10%

4 Install fuse holder

Reinsert fuse holders in the instrument (the arrows printed on the holders must point in the same direction as the arrows on the inside of fuse cover 10).

5 Install fuse cover

Push in fuse cover 10 firmly until it clicks into place.

6 Connect mains cable

Plug mains cable into mains connection plug 10.

818 IC Pump / 8.818.1023 Instructions for Use

2 Installation

2.6.2 Fuses

The 818 IC Pump contains two fuses built in as standard, either of type

0.25 AT (for 230 V: 0.25 A, slow-blow, Metrohm ordering number U.600.0010) or 0.5 AT (for 110 V: 0.5 A, slow-blow, Metrohm ordering number U.600.0013). Ensure that the two fuses correspond to the fuse type specified for the local mains voltage (see also Fig. 2).

Ensure that the instrument is never put into operation with fuses of another type, otherwise there is danger of fire!

To change wrong or blown fuses, proceed as described in section

2.6.1.

2.6.3 Mains cable

The instrument is supplied with one of three mains cables:

• 6.2122.020 with plug SEV 12 (Switzerland, …)

• 6.2122.040 with plug CEE(7), VII (Germany, …)

• 6.2133.070 with plug NEMA 5-15 (USA, …)

which are three-cored and fitted with a plug with an earthing pin. If a different plug has to be fitted, the yellow/green lead (IEC standard) must be connected to protective earth (protection class 1).

Any break in the earthing inside or outside the instrument can make it a hazard!

Plug the mains cable into mains connection plug 10 (see Fig. 2).

2.6.4 On/off switching of the instrument

The 818 IC Pump is switched on and off using mains switch 10 (see Fig. 2). When the instrument is switched on the mains pilot lamp 1 lights up.

818 IC Pump / 8.818.1023 Instructions for Use

2.7 Software installation

The PC program «IC Net 2.3» is required for the operation of the 818 IC Pump. This program runs under Windows 2000 and Windows XP operating systems and is installed according to section 1.4.2 of the

Instructions for Use 8.110.8283 from the Metrodata software «IC Net

2.3».

The installation of 818 IC Pump is described in section 6.1.2 of the

Instructions for Use 8.110.8283 from the Metrodata software «IC Net

2.3». Take care that the RS 232 settings are identical for the RS 232

interface of 818 IC Pump and 830 IC Interface. For the settings of 818 IC Pump see section 6.10.

2.8 Deaerating the pump

1 Start IC system

Switch the IC instruments on, start «IC Net» and open the device window

818 IC Pump.

Operation of 818 IC Pump via the software «IC Net 2.3» is described more detailed in section 3. For further information refer to the Instructions for Use of «IC Net» and to the online help of the program.

2 Purge pump

The purge valve 2 is opened by turning anticlockwise and the pump can now be deaerated by starting the pump in «IC Net».

The solvent bottle should be located somewhat above the pump, but

at least at the same level as the pump head pump).

3 Deaerate pump

Should the pump not prime by itself, the 6.2816.020 syringe supplied is used to siphon off solvent via the outlet capillary 5 with opened purge valve through the pump head until there are no more bubbles in the solvent entering the syringe. The pump can then again be briefly purged.

4 Close purge-valve

On completion of this procedure, the purge valve 2 is closed by turning clockwise. The pump is now ready for operation.

(never below the

818 IC Pump / 8.818.1023 Instructions for Use

3 Operation

The operation of 818 IC Pump is made completely via the Metrohm software «IC Net».

This section describes only the most important points concerning the operation of the 818 IC Pump. For further details please refer to the «IC Net» Instructions for Use and to the on-line help in the PC program.

3.1 Switch instrument on/off

The 818 IC Pump is switched on and off using mains switch 10 on the rear of the instrument (sse Fig. 2). When the instrument is switched on the mains pilot lamp 1 lights up to show that the instrument is ready for use.

3.2 818 IC Pump icon

818 IC Pump

3.3 Settings in the "818 IC Pump" window

By double-clicking the 818 icon by clicking this icon with the right mouse button and selecting the window for parameter settings is opened. It consists of the four tabs

Settings, Program, Links and Advanced.

Open menu item the 818 IC Pump

818 IC Pump / 8.818.1023 Instructions for Use

3.3 Settings in the "818 IC Pump" window

Settings

Settings tab of the 818 IC Pump window contains basic settings of

The the 818 IC Pump.

Pressure Live display of current pressure in MPa. Flow Live display of current flow in mL/min.

New flow A new flow can be set by moving the scroll bar,

by entering a value or by changing the value using the up and down arrows. Entry range:

Min/Max pressure The minimum and maximum pressure limit for

0.01 ... 5.00 mL/min

the 818 IC Pump can be set in steps of 0.1 MPa. Entry range:

0.0 ... 30.0 MPa.

The set maximum limit value should lie

between 5 to 10 MPa above the particular operating pressure or the maximum admissible operating pressure of the column used. If the pump exceeds the preset limit value during operation, it is switched off immediately. At the same time, the

Pressure field color in the 818

settings window is changed to the value set for

Out of range.

The set minimum limit value should lie far

enough below the particular operating

pressure. If the pump pressure falls below this preset lower limit during operation and this pressure drop persist for several revolutions of the pump cam due to leaks or interrupted inflow of the eluent, the pump is automatically

818 IC Pump / 8.818.1023 Instructions for Use

3 Operation

switched off. At the same time, the Pressure field color in the 818 settings window is changed to the value set for

<Set> Send current parameters immediately to the

Out of range.

818 IC Pump (only available if pump is running). Parameters are not stored in the system file (

*.smt) as long as the file is not

saved.

<Start> Set current parameters and start 818 IC Pump

drive.

<Stop> Stop 818 IC Pump drive.

Time program

Program tab of the 818 IC Pump window a user-defined time program

The can be entered. This program is started automatically as defined in the

Start mode window (see section 4.4.3 «IC Net 2.3» Instructions for Use

8.110.8283), either at the moment the determination is started (

determination

) or at the moment the sample is injected (Start with inject).A

Start with

First column Time at which program instruction is applied.

Entry range:

0.0 ... 999.9 min

If no time is entered, the program instruction is applied together with the last instruction with time entry.

Second column Program instruction (see below). Third column Parameter for program instruction (see below).

818 IC Pump / 8.818.1023 Instructions for Use

3.3 Settings in the "818 IC Pump" window

ENABLED Enable program start (a disabled program is

not started).

<Add> Add new program instruction. <Delete> Delete selected program instruction. <Verify> Test the time program (error messages are

displayed if program is wrong).

List of program instructions

The following program instructions can be added to the time program on the

Befehl Parametereintrag Bedeutung

Pump on, off Switch the high pressure pump on or

Program page:

off.

Flow 0.01 ... 5 mL/min Set flow rate of the high pressure

pump to the desired value.

Links

Pmax 0.0 ... 30.0 MPa Set maximum pressure limit for the

high pressure pump to the desired value.

Pmin 0.0 ... 30.0 MPa Set minimum pressure limit for the

high pressure pump to the desired value.

Die Registerkarte Einstellung der COM-Schnittstelle (Details siehe section 5.2.4 Links of the «IC Net 2.3» Instructions for Use 8.110.8283).

Links im Fenster 818 IC Pump dient zur Wahl und

818 IC Pump / 8.818.1023 Instructions for Use

3 Operation

Advanced

Advanced tab of the 818 IC Pump window is only available for a

The

connected system.

Correction factor Flow correction factor to adjust the displayed

flow to the actual flow measured with the aid of a measuring cylinder. Entry range:

Correction factor =

0.90 ... 1.10

Displayed flow rate (in mL/min)

Measured flow rate (in mL/min)

<Set> Send current Correction factor value to the 818

IC Pump. This parameter is not stored in the system file (

*.smt) as long as the file is not

saved.

The difference between displayed and actual flow rate can be maximum 3 % owing to unavoidable tolerances in the manufacture of mechanical parts. Examine therefore the flow rate after disassembling/assembling of the pump head and adjust the 818 IC Pump with the correction factor if necessary.

818 IC Pump / 8.818.1023 Instructions for Use

4.1 Practical notes

4 Notes – Maintenance – Faults

4.1 Practical notes

4.1.1 Protection against foreign particles

To protect the column against foreign particles which could have an adverse influence on the separation efficiency, we advise you to subject both the eluents and all samples to microfiltration (0.45 µm filter) and to siphon the eluent through the 6.2821.090 Aspirating filter.

To avoid contamination by abrasive particles arising from piston seals of the 818 IC Pump, it is advantageous to install an in-line filter 24 between the pump and the 820 IC Separation Center (see Fig. 5). We recommend to use the 6.2821.100 Filter unit PEEK which is included in the standard accessories of the 818 IC Pump (see section 2.3.3).

4.1.2 Pulsation dampener

To protect the column material against pressure shocks caused by injection we recommend to use a pulsation dampener between the 818 IC Pump and the 820 IC Separation Center (see Fig. 5). The optional

6.2620.150 Pulsation dampener MF (see section 2.3.2) is eminently suitable for this purpose.

4.1.3 Eluents

Treatment

For the preparation of the eluents one should use chemicals of a purity degree of at least "p.a.". For dilution please use only high purity water.

Fresh eluents should always be microfiltered (0.45 µm filter) and

degassed (with N 837 IC Eluent Degasser is available from Metrohm. The eluent should

be continuously stirred with a magnetic stirrer, particularly when the recycling procedure is employed or when alkaline eluents are used. For alkaline eluents and eluents with low buffering capacity one should preferably use CO

The supply vessel containing the eluent must be closed as tightly as possible to avoid excessive evaporation. This is primarily important with eluents containing organic solvents (e.g. acetone), the evaporation of which can lead to drifts in the long term. If work is performed in a very sensitive range, even if one drop of condensate falls back in the eluent this can cause a noticeable change in the background conductivity.

, He or vacuum). For in-line degassing of eluents the

absorbers.

Precipitates

Salt crystals between the piston and the seal are the cause of abrasive particles, which can enter the eluent. These lead to contaminated

818 IC Pump / 8.818.1023 Instructions for Use

4 Notes – Maintenance – Faults

valves, pressure rise and in extreme cases to scratched pistons. It is thus essential to ensure that no precipitates can appear, e.g. when the eluent is changed. Solutions used in direct succession must therefore be miscible. If the system has to be rinsed with an organic solution, several solvents with increasing or decreasing lipophilic character may possibly have to be used (e.g. water ↔ acetone ↔ chloroform).

4.2 Maintenance and servicing

4.2.1 General information

Care

The 818 IC Pump requires proper care and attention. Excessive contamination of the instrument could possibly lead to malfunctions and a shorter service life of the inherently rugged mechanical and electronic parts.

Spilled chemicals and solvents should be wiped up immediately. It is especially important to protect the plug connections at the rear of the instrument (particular the mains plug) against contamination.

Although constructional measures have been designed to virtually eliminate such a situation, should corrosive media penetrate the interior of the instrument the mains plug of the 818 IC Pump must be immediately disconnected to prevent extensive damage to the instrument electronics. Inform Metrohm service if your instrument has been damaged in such a way.

The instrument must not be opened by untrained personnel. Please comply with the safety notes in section Fehler! Verweisquelle konnte nicht gefunden werden..

Maintenance by Metrohm service

Maintenance of the 818 IC Pump is best done as part of an annual service performed by specialists from the Metrohm company. If work is frequently performed with caustic and corrosive chemicals, it may be necessary to shorten the interval between servicing.

The Metrohm service department is always willing to offer expert advice on the maintenance and servicing of all Metrohm instruments.

818 IC Pump / 8.818.1023 Instructions for Use

4.2 Maintenance and servicing

4.2.2 Maintenance work at the pump head

In many cases, an unstable baseline (pulsation, flow fluctuations) can be traced to contaminated valves or faulty, leaky piston seals. For cleaning contaminated valves and/or replacement of wear parts such as pistons, piston seals and valves, proceed as follows:

1 Detach pump head

Unscrew connection capillary 6 from the pump head 8 with the aid of the ¼" wrench (see Fig. 1). Then remove the pump head 8 from the pump housing by loosening the 4 hexagon screws 9 using the 6.2621.030 hexagon key. The main piston is on the left (when viewed from front), the auxiliary piston on the right.

2 Disassemble pump head

Strip down pump head 8 in accordance with Fig. 8. Main and auxiliary pistons are identical with the following exceptions:

• The spring 29 of the auxiliary piston (right piston) is more

powerful (longer) than that of the main piston (left piston).

• Inlet and outlet valve are not present in the secondary

cylinder.

To prevent the piston

, the screw 26 must be undone very carefully by hand.

suddenly jumping out of the piston cartridge

3 Cleaning/replacement of piston 27

Pistons contaminated by abrasive particles or deposits are cleaned with scouring powder and rinsed free of any particles with dist. water. Relatively badly contaminated or scratched pistons must be replaced (spare part: 6.2824.070 Zircon piston).

4 Replacement of piston seal 34

To remove damaged piston seals 34 the special tool 38 is used. This is screwed into the seal 34, which can then be pulled out (see Fig. 9A).

When the special tool is completely destroyed!

is screwed into the piston seal

the latter

818 IC Pump / 8.818.1023 Instructions for Use

4 Notes – Maintenance – Faults

27 26 34 333229 35

Fig. 8: Components of the pump head

30 31

Fig. 9: Replacement of the piston seal

818 IC Pump / 8.818.1023 Instructions for Use

4.2 Maintenance and servicing

Pump head 6.2824.100

Screw

for piston cartridge 30.

Piston with piston shaft (6.2824.070

zircon piston or 6.2824.000 sapphire piston)

Spring retainer

Spring (6.2824.050) (for main piston)

or Spring (6.2824.060) (for auxiliary piston)

Piston cartridge (4.709.0760)

Piston guide sleeve (4.709.4380)

Sapphire supporting ring

To install a new piston seal 34 the special tool 39 is used:

(6.2824.030)

Piston guide sleeve (4.709.4370)

Piston seal 6.2741.020

Inlet valve (6.2824.090)

Outlet valve (6.2824.080)

Screw holder for valve.

Special tool (6.2617.010) to remove

the piston seal 34.

Special tool (6.2617.010) to install

the piston seal 34.

• First the new seal is inserted firmly in the recess of tool 39 by

hand (see Fig. 9B). The seal spring must be located on the outside.

• The tool 39 together with the seal is then inserted in the pump

head 8 and the seal pressed into the pump head recess with the aid of tool 38 (see Fig. 9C).

The seal surface in the pump head contact with tool)!

must not be damaged (avoid

818 IC Pump / 8.818.1023 Instructions for Use

4 Notes – Maintenance – Faults

5 Cleaning/Replacement of inlet valve 35 and outlet

valve 36

Contaminated or blocked valves are cleaned by rinsing with dist. water, RBS solution or acetone. The rinsing effect can be reinforced by brief treatment in an ultrasonic bath (max. 20 s; if longer the sapphire sphere of the valve can be damaged).

If this does not have the desired effect, the valves can be disassembled as shown in Fig. 10. Therefore the valves are inserted into the holder of 6.2617.020 special tool and the components of the valve are pushed out with the aid of the push rod (also included in 6.2617.020 special tool) inserted through the upper opening in the valve housing 40. The individual components are rinsed with dist. water and/or acetone, and the sapphire sphere cleaned with a paper towel. The valve is then reassembled in accordance with Fig. 10. The components of the inlet and outlet valves are identical, they are distinguished only by the positioning of the sapphire sleeve 43 and the ceramic holder 45 (see Fig. 10).

Valves that fail to function faultlessly after such cleaning must be replaced.

In the reinstallation of the inlet valve 35 or the outlet valve 36 on no account must the two outwardly identical valves be interchanged. To determine which valve is which, note that the liquid flows through the pump head from the bottom up. The flow direction of the valves can be checked simply by blowing through the clean valve. Both valves are installed with the black face in the direction of the pump head (see Fig. 8).

If by mistake an inlet valve

, an extreme pressure buildup occurs within the working cylinder,

which is not detected by the pressure transducer and will destroy the

piston seal

is installed instead of the outlet valve

6 Mounting the pump head

The components of the pump head 8 are reassembled as shown in Fig. 8. the screw 26 and the piston cartridge 30 may be tightened only by hand. On the other hand, the two valve screw holders 37 are tightened with a wrench. The pump head is then remounted on the pump (see section 2.2).

818 IC Pump / 8.818.1023 Instructions for Use

4.2 Maintenance and servicing

Inlet valve Outlet valve

6.2824.090 6.2824.080

Valve housing

Sealing ring (black)

Sleeve

Sapphire sleeve

The bright side must point towards the sapphire sphere

Sapphire sphere

Ceramic holder for sapphire

sphere

The large recess must point in the direction of the sapphire sphere

Sealing

The larger opening must point outwards

Fig. 10: Components of inlet valve

and outlet valve 36

818 IC Pump / 8.818.1023 Instructions for Use

4 Notes – Maintenance – Faults

4.3 Faults and malfunctions

If difficulties appear with the IC system during analyses, their causes are best investigated in the order separating column → 818 IC Pump

→ eluent → 819/820 IC System (see 819/820 Instructions for Use, section Faults and malfunctions). Several of the malfunctions which may

appear with the 818 IC Pump are listed in the following table with details of possible causes and countermeasures.

Malfunction Cause Rectification

Baseline with high noise level, pulsation

Drift of baseline • Thermal equilibrium

Considerable pressure drop

Considerable pressure rise

Pump is not recognized in «IC Net»

• Contaminated pump

values

• Faulty piston seals

• Quality of the pump

does not suffice for the selected sensitivity

not yet reached

• Leak in system

• Evaporation of organic

solvent in eluent

• Leak in System • Check connections

• Contamination of the

filter in the 6.2821.100 Filter unit PEEK

• No RS connection • Switch instrument on.

• Clean the valves (see

section 4.2.2)

• Replace the piston

seals (see section 4.2.2)

• Use pulsation

dampener, use more powerful pump or lower the sensitivity

• Condition system with

820 heating switched on

• Check connections

and make leakproof

• Ensure better closure

of eluent supply vessel, stir

and make leakproof

• Replace 6.2821.130

connector with filter

• Check wiring.

• Check that the

connection specified in the 818 IC Pump window (see section

3.3 Links) matches the actual wiring

818 IC Pump / 8.818.1023 Instructions for Use

5.1 RS 232 interface

5 Interfaces

5.1 RS 232 interface

5.1.1 Data transmission protocol

The RS 232 interface of the 818 IC Pump is configured as DTE (Data Terminal Equipment) with the following technical specifications:

• Standard Data interface in accordance with EIA standard

RS 232C (DIN 66020, page 1)

• Baud rate 9600

• Data Bits 8

• Stopp Bits 1

• XON/XOFF off

• Control characters C

• Max. line length 80 characters + C

• Cable For interconnections of the 818 IC Pump with

• Cable length max. ca. 15 m

DEC 13 HEX 0D

DEC 10 HEX 0A

R LF

non-Metrohm units, only a shielded data cable (e.g. Metrohm D.104.0201) may be used. The cable shielding must be faultlessly earthed at both units (pay attention to current loops; always use star-head earthing). Only connectors with adequate shielding may be used (e.g. Metrohm K.210.0001 with K.210.9004).

818 IC Pump / 8.818.1023 Instructions for Use

5 Interfaces

5.1.2 Pin assignment

RS 232C interface extern

Transmitted Data (TxD) If no data transmission takes place, the line is maintained in the ”ON” condition. Data are sent only when CTS and DSR are in the ”ON” condition and DCD is in the ”OFF” condition.

Received Data (RxD) Data are received only when DCD is ”ON”.

Request to Send (RTS) ON condition: 818 IC Pump is ready to send data.

Clear to Send (CTS) ON condition: Remote station is ready to receive data.

Data Set Ready (DSR) ON condition: The transmission line is connected.

Signal Ground (GND)

Data Carrier Detect (DCD)

ON condition: The received signal level is within the tolerance range (remote station is ready to send data).

Pin 2

Transmitted Data

Pin 3

Received Data

Pin 4

Request to Send

Pin 5

Clear to Send

Pin 6

Data Set Ready

Pin 7

Signal Ground

Pin 8

Data Carrier Detect

Data Terminal Ready (DTR) ON condition: 818 IC Pump is ready to receive data.

Protective earth

Direct connection from cable connector to protective earth of the device.

Contact arrangement at the connector (female) for socket "RS 232" (male)

1 13

Polarity assignment of the signals

• Data lines (TxD, RxD) Voltage negative (<–3 V): signal status ”ONE” Voltage positive (>+3 V): signal status ”ZERO”

• Control or message lines (CTS, DSR, DCD, RTS, DTR) Voltage negative (<–3 V): OFF status Voltage positive (>+3 V): ON status

In the transition region from +3 V to –3 V, the signal status is undefined.

Driver 14C88 to EIA RS 232C specification

Connection cables to external devices must have an appropriate 25-pin connector (female). Metrohm offers connector sockets (K.210.9004) and the associated housing (K.210.0001) as an option for the preparation of such cables.

14 25

Receiver 14C89 to EIA RS 232C specification

No liability whatsoever will be accepted for damage arising from the improper connection of devices.

Pin 20

Data Terminal Ready

818 IC Pump / 8.818.1023 Instructions for Use

5.2 Control interface

5.2.1 Functions

The 818 IC Pump has the following control inputs and outputs, which are accessible via the control interface:

Inputs Reduce flow rate via contact

Stop pump via contact

Outputs Contact open, when pump has been stopped

You will find detailed information on these functions in the following section.

5.2.2 Control inputs and outputs

818 IC Pump external Function

Inputs

+ 5V

100k

4.7k 2

Reduced flow rate (Reduced Flow) with contact

Contact open: Flow rate 100%

Contact closed: Flow rate 10%

818 IC Pump / 8.818.1023 Instructions for Use

5 Interfaces

818 IC Pump external Function

Inputs

+ 5V

100k

4.7k 8

Stop pump with contact

Contact closed: Pump is stopped

Outputs

Pump stopped

Contact open: Pump stopped

Contact closed: Pump running

Relay

818 IC Pump / 8.818.1023 Instructions for Use

5.2 Control interface

818 IC Pump external Function

Display of suction phase of the 818 IC Punp

Connection not used

Contact arrangement at connector (male) for socket "Control" (female)

5 1

No liability whatsoever will be accepted for damage arising from the improper connection of devices.

818 IC Pump / 8.818.1023 Instructions for Use

6 Appendix

6.1 Technical specifications

Type Serial dual piston with two valves

Pump capacity

Flow range 0.05...5.00 mL/min

Flow accuracy < 1.0 %

Flow constancy < 0.5 % (after unit warmed up)

Reproducibility of eluent flow

Pressure

Pressure range 0...30.0 MPa (0...300 bar)

Resolution for pressure indicator

Pressure measurement Piezoresistive measurement principle

Accuracy of pressure measurement

Residual pulsation < 1 %

Recorder output for pressure indication

Provided that nothing to the contrary is mentioned, the published data are typical values for the 818 IC Pump at an ambient temperature of 25°C.

< 0.1 %

0.1 MPa (1 bar)

Response time: 3 ms Measurement volume: ca. 50 µL

< 3 %

1 mV/MPa (1 mV/10 bar) Time constant switchable: HIGH = 20 ms

LOW = 1 s

Safety shutdown

Function Automatic shutdown when upper and lower

pressure limits violated

Maximum pressure limit Adjustable between 0.1...30.0 MPa (1...300 bar)

Response time: 1 pump cycle

Minimum pressure limit Adjustable between 0.1 ... 30.0 MPa (1...300 bar),

inactive at 0 bar Response time: 3 pump cycles The cutoff function first becomes active 2 min after the pump flow has been switched on.

818 IC Pump / 8.818.1023 Instructions for Use

6.1 Technical specifications

Pump head

Pump head volumes Main piston: 40 µL

Priming piston: 20 µL

Pump displacement volumes Main piston: 28.5 µL

Priming piston: 14.25 µL

Length of stroke Main piston: 3.6 mm

Priming piston: 1.8 mm

Programming

Remote control Flow rate

Maximum and minimum pressure limit values Correction factor for pump head Start/Stop

Storage of entries

Type Flash-ROM

Duration > 20 years

Remote control

Interfaces RS 232 (syntax Metrohm, see section 5.1, details

on request) Current Loop (details on request) Control (see section 5.2)

RS 232 interface

Standard EIA RS 232C, CCITT V.24, ISO 2110, DIN 66020

Plug

D-Sub 25 pins, male

Mode Full duplex

(simultaneous sending and receiving)

Parameters Baud rate: 9600

Handshake: no Handshake Parity: none Stop bits: 1 Data bits: 8

Mains connection

Voltage 115 V: 100...120 V ± 10 %

230 V: 220...240 V ± 10 % switchable

Frequency 50...60 Hz

Power consumption 50 VA

Fuses Diameter = 5 mm, length = 20 mm

818 IC Pump / 8.818.1023 Instructions for Use

100…120 V: 0.5 A (slow blow) 220…240 V: 0.25 A (slow blow)

6 Appendix

Safety specifications

Construction / Testing According to EN/IEC 61010-1 / UL 3101-1,

protection class 1, degree of protection IP40

Safety directions This Instructions for Use include information and

warnings which must be heeded by the user to assure safe operation of the instrument.

Electromagnetic compatibility (EMC)

Emitted interference Standards met:

- EN/IEC 61326

- EN 55022

- CISPR 22

Immunity to interference Standards met:

- EN/IEC 61326

- EN/IEC 61000-4-2

- EN/IEC 61000-4-3

- EN/IEC 61000-4-4

- EN/IEC 61000-4-5

- EN/IEC 61000-4-6

- EN/IEC 61000-4-11

- EN/IEC 61000-4-14

- NAMUR

Ambient temperature

Nominal operating range +5…+45°C

(at 5…85 % atmospheric humidity)

Storage, transport –20…+70°C

Diagnosis

Self-diagnostic test Automatic self-diagnosis when instrument

switched on

Materials (in contact with solvent)

Sapphire, ruby, ceramic, PCTFE, PTFE, PEEK

Housing

Material of cover Polyurethane rigid foam (PUR) with fire protection

for fire class UL94VO, FCH-free

Material of base Steel enamelled

Width 260 mm

Height 130 mm

Depth 385 mm (with pump head)

Weight 10 kg (incl. accessories)

818 IC Pump / 8.818.1023 Instructions for Use

6.2 Scope of delivery

⁄

6.2 Scope of delivery

Subject to changes ! All dimensions are given in mm.

The IC Pump 2.818.0110 (metal-free) includes the following parts:

Quant. Order No. Description

1 6.1831.010 PEEK capillary

Length = 3 m

1 6.1834.000 Aspirating tubing

made of PTFE, with connector for

6.2821.090 Aspirating filter

Length = 1.2 m

For the connection 818 IC Pump – eluent container

1 6.2122.0X0 Mains cable

according to customer's specification:

Cable socket Cable plug

Type IEC 320/C 13 Type SEV 12 (CH…)............................... 6.2122.020

Type IEC 320/C 13 Type CEE (7), VII (D…)........................... 6.2122.040

Type CEE (22), V Type NEMA 5-15 (USA…) ...................... 6.2122.070

0.25

16"

2.51.5

1 6.2617.010 Special tool

For removing the piston seal of the pump head

1 6.2621.000 Adjustable spanner

1 6.2621.030 Hexagon key 4 mm

For mounting the pump head of the 818 Pump.

1 6.2744.010 PEEK compression fitting

For the connection of 6.1831.010 PEEK capillaries Set of 5

35 50

max.

150

M3.5

29.5

818 IC Pump / 8.818.1023 Instructions for Use

6 Appendix

Quant. Order No. Description

1 6.2816.020 Syringe

made of PP, volume = 10 mL

with Luer connector

1 6.2816.040 Tubule

with PTFE tubing and Luer connection

For 6.2816.020 syringe

1 6.2821.090 Aspirating filter

Pore dimension 20 µm

For 6.1834.000 Aspirating tubing.

Set of 5

1 6.2821.120 Filter unit PEEK 2 µm

To avoid contamination due to abrasive particles of piston seals

Spare part: 6.2821.130 Filter

1 6.2824.100 Pump head PEEK (metal-free)

Complete, with fixation screws

102

120

∅9

1 8.818.1023 Instructions for Use (English)

for 818 IC Pump

818 IC Pump / 8.818.1023 Instructions for Use

6.3 Optional accessories

Order No. Description

6.1834.010 Aspirating tubing

made of PTFE, with connector for 6.2821.090 Aspirating filter

Length = 2.5 m

For the connection 818 IC Pump – eluent vessel of 6.5324.000 Bottle rack

6.2620.150 Pulsation dampener MF

Metal-free pulsation dampener for reduction of pulsation and preservation of separating columns.

2.51.5

6.2621.080 Capillary tubing cutter

for 6.1831.010 PEEK capillaries and

6.1822.010 PTFE micro capillaries

incl. 5 additional blades

6.2741.020 Piston seal PE

Spare part for 6.2824.100 pump head (standard seal)

6.2741.010 Piston seal PE

Spare part for 6.2824.100 pump head (only suitable for aqueous eluents)

6.2744.070 PEEK compression fitting (short)

Spare part for 6.2824.100 pump head Set of 5

118

6.5

6.2821.130 Filter for filter unit PEEK 2 µm

Spare part for 6.2821.120 Filter unit PEEK Set of 10

6.2824.070 Zircon piston

Spare part for 6.2824.040 and

6.2824.100 pump head

818 IC Pump / 8.818.1023 Instructions for Use

∅3

65.5

6 Appendix

Order No. Description

6.2824.030 Sapphire supporting ring

Spare part for 6.2824.100 pump head

6.2824.050 Spring for main piston

Spare part for 6.2824.100 pump head

6.2824.060 Spring for auxiliary piston

Spare part for 6.2824.100 pump head

6.2824.080 Outlet valve

Spare part for 6.2824.100 pump head

6.2824.090 Inlet valve

Spare part for 6.2824.100 pump head

1.3

∅3.2

∅6.3

∅11

∅8

6.2617.020 Special tool For dismantling the valves

6.5324.000 Bottle rack

For fixing 3 supply bottles for elent, regeneration and rinsing solution, incl. accessories (bottles, siphon, etc.)

6.2115.040 Connection cable

For the connection 818 IC Pump – recorder

For plotting curves delivery vs time.

6.2134.080 RS 232 connection cable

Connection cable 818 IC Pump – 830 IC Interface

6.2134.090 RS 232 connection cable

Connection cable 830 IC Interface – 2 external devices (818, 819, etc.)

818 IC Pump / 8.818.1023 Instructions for Use

6.3 Optional accessories

Order No. Description

6.2125.110 RS 232 connection cable

Connection cable 818 IC Pump – PC)

6.2125.060 RS 232 connection cable

Connection cable 818 IC Pump – PC.

818 IC Pump / 8.818.1023 Instructions for Use

6 Appendix

6.4 Validation / GLP

GLP (Good Laboratory Practice) requires, among other things, that the precision and correctness of analytical instruments is checked at regular intervals by using SOPs (Standard Operating Procedures, SOP). An example of such a standard operating procedure is available from Metrohm under the title «Application Bulletin No. 277 –

Validation of Metrohm Ion Chromatography Systems by using Standard Operating Procedures (SOP)». This SOP can be adapted

for your ion chromatography system and used for its validation.

The 818 IC Pump must be included as a part of the whole ion chromatography system, whose most important components include pumps, separation columns, detector and evaluation system, in the allembracing validation of the whole system.

Please contact your local Metrohm agency in order to receive support in validating your 818 IC Pump. It can also provide you with validation documentation which will help you to carry out your installation qualification (IQ) and operational qualification (OQ).

Further information about QA, GLP and validation can also be found in the brochure «Quality management with Metrohm» which is also obtainable from your local Metrohm agency.

Checking the electronic and mechanical assemblies of Metrohm instruments can and should be undertaken within the framework of regular servicing by Metrohm technicians. All Metrohm instruments are equipped with start-up check routines which check that the relevant assemblies are functioning perfectly when the instrument is switched on. If no error message appears it can be assumed that the instrument is functioning properly.

The 818 IC Pump also contains a built-in diagnosis program which allows the service technicians to check the functioning of particular assemblies should faults or malfunctions occur and to localize them.

818 IC Pump / 8.818.1023 Instructions for Use

6.5 Warranty and Conformity

6.5.1 Warranty

The warranty on our products is limited to defects that are traceable to material, construction or manufacturing error which occur within 12 months from the day of delivery. In this case, the defects will be rectified in our workshops free of charge. Transport costs are to be paid by the customer.

For day and night operation, the warranty is limited to 6 months.

Glass breakage in the case of electrodes or other parts is not covered by the warranty. Checks which are not a result of material or manufacturing faults are also charged during the warranty period. For parts of outside manufacture insofar as these constitute an appreciable part of our instrument, the warranty stipulations of the manufacturer in question apply.

With the regard to the guarantee of accuracy, the technical specifications in the instruction manual are authoritative.

Concerning defects in material, construction or design as well as the absence of guaranteed features, the orderer has no rights or claims except those mentioned above.

If damage of the packaging is evident on receipt of a consignment or if the goods show signs of transport damage after unpacking, the carrier must be informed immediately and a written damage report demanded. lack of an official damage report releases Metrohm from any liability to pay compensation.

If any instruments and parts have to be returned, the original packaging should be used if at all possible. This applies above all to instruments, electrodes, burette cylinders and PTFE pistons. Before embedment in wood shavings or similar material, the parts must be packed in a dustproof package (for instruments, use of a plastic bag is imperative). If open assemblies are enclosed in the scope of delivery that are sensitive to electromagnetic voltages (e.g. data interfaces etc.) these must be returned in the associated original protective packaging (e.g. conductive protective bag). (Exception: assemblies with built-in voltage source belong in a non-conductive protective packaging).

No warranty responsibility whatsoever will be accepted by Metrohm for damage which arises as a result of non-compliance with these instructions.

818 IC Pump / 8.818.1023 Instructions for Use

6 Appendix

6.5.2 Declaration of Conformity

This is to certify the conformity to the standard specifications for electrical appliances and accessories, as well as to the standard specifications for security and to system validation issued by the manufacturing company.

Name of commodity 818 IC Pump

System software Stored in ROMs

Name of manufacturer Metrohm Ltd., Herisau, Switzerland

Description

Dual piston pump for use in Ion Chromatography (IC) and High Performance Liquid Chromatography (HPLC).

This Metrohm instrument has been built and has undergone final type testing according to the standards:

CH-9101 Herisau, Switzerland Tel. +41 71 353 85 85 Fax +41 71 353 89 01 www.metrohm.com

Electromagnetic compatibility: Emission EN/IEC 61326, EN 55022 / CISPR 22

Electromagnetic compatibility: Immunity

EN/IEC 61326, EN/IEC 61000-4-2, EN/IEC 61000-4-3, EN/IEC 61000-4-4, EN/IEC 61000-4-5, EN/IEC 61000-4-6, EN/IEC 61000-4-11, EN/IEC 61000-4-14, NAMUR

Safety specifications EN/IEC 61010-1, UL 3101-1 protection class I

It has also been certified by ElectroSuisse, which is member of the International Certification Body (CB/IEC).

The instrument meets the requirements of the CE mark as contained in the EU directives 89/336/EEC and 73/23/EEC and fulfils the following specifications:

EN 61326 Electrical equipment for measurement, control and laboratory use – EMC requirements EN 61010-1 Safety requirements for electrical equipment for measurement, control and laboratory

use

Metrohm Ltd. is holder of the SQS-certificate of the quality system ISO 9001 for quality assurance in design/development, production, installation and servicing.

The system software, stored in Read Only Memories (ROMs) has been validated in connection with standard operating procedures in respect to functionality and performance. The technical specifications are documented in the instruction manual.

Herisau, May 28, 2003

Dr. J. Frank Ch. Buchmann Development Manager Production and Quality Assurance Manager

818 IC Pump / 8.818.1023 Instructions for Use

6.5 Warranty and Conformity

6.5.3 Quality Management Principles

Metrohm Ltd., CH-9101 Herisau, Switzerland

CH-9101 Herisau/Switzerland E-Mail info@metrohm.com Internet www.metrohm.com

Metrohm Ltd. holds the ISO 9001 Certificate, registration number 10872-02, issued by SQS (Swiss Association for Quality and Management Systems). Internal and external audits are carried out periodically to assure that the standards defined by Metrohm’s QM Manual are maintained.

The steps involved in the design, manufacture and servicing of instruments are fully documented and the resulting reports are archived for ten years. The development of software for PCs and instruments is also duly documented and the documents and source codes are archived. Both remain the possession of Metrohm. A non-disclosure agreement may be asked to be provided by those requiring access to them.

The implementation of the ISO 9001 quality system is described in Metrohm’s QM Manual, which comprises detailed instructions on the following fields of activity:

Instrument development

The organisation of the instrument design, its planning and the intermediate controls are fully documented and traceable. Laboratory testing accompanies all phases of instrument development.

Software development

Software development occurs in terms of the software life cycle. Tests are performed to detect programming errors and to assess the program’s functionality in a laboratory environment.

Components

All components used in the Metrohm instruments have to satisfy the quality standards that are defined and implemented for our products. Suppliers of components are audited by Metrohm as the need arises.

Manufacture

The measures put into practice in the production of our instruments guarantee a constant quality standard. Production planning and manufacturing procedures, maintenance of production means and testing of components, intermediate and finished products are prescribed.

Customer support and service

Customer support involves all phases of instrument acquisition and use by the customer, i.e. consulting to define the adequate equipment for the analytical problem at hand, delivery of the equipment, user manuals, training, aftersales service and processing of customer complaints. The Metrohm service organisation is equipped to support customers in implementing standards such as GLP, GMP, ISO 900X, in performing Operational Qualification and Performance Verification of the system components or in carrying out the System Validation for the quantitative determination of a substance in a given matrix.

818 IC Pump / 8.818.1023 Instructions for Use

6 Appendix

6.6 Index

818 IC Pump

Advanced...................................22

Links ...........................................21

program .....................................20

program instructions.................. 21

830 IC Interface

Connection................................. 13

Abrasive particles ..........................23

<Add>............................................21

Adjustable spanner (6.2621.000) .. 39

Ambient temperature ..................... 38

Appendix ........................................ 36

Arrangement of the instruments ...... 7

Aspirating capillary 7

Figure ........................................... 2

Mounting the suction tubing......12

Aspirating filter (6.2821.090)

Mounting .................................... 12

Ordering designation ................. 40

Practical notes ...........................23

Aspirating tubing (6.1834.000) ........9

Mounting .................................... 12

Ordering designation ................. 39

Aspirating tubing (6.1834.010)

Mounting .................................... 12

Ordering designation ................. 41

Attaching the accessories ...............9

Attention ........................................... 5

Baud rate ........................................31

Bottle rack (6.5324.000)

Mount aspirating tubing............. 12

Ordering designation ................. 42

Cable

6.2115.040 ................................ 42

6.2121.110 .................................43

6.2125.060 ...........................14, 43

6.2125.110 .................................14

6.2134.000 .................................42

6.2134.080 ...........................13, 42

6.2134.090 .................................13

Capillaries ........................................9

Capillary tubing cutter

(6.2621.080)...............................9, 41

Care................................................24

CE mark .........................................46

Ceramic holder 45

Figure ......................................... 29

Check ............................................... 7

Compression fitting 20

6.2744.010 .............................9, 10

Mounting ...................................... 9

Ordering designation ................. 39

Connecting the tubing ...................11

Connection

830 IC Interface.......................... 13

Modular IC system..................... 13

PC...............................................14

Connection capillary 3

Connection 818 – 820................11

Figure ...........................................2

Mounting ....................................11

Connection capillary 4

Figure ...........................................2

Connection capillary 6

Detaching ...................................25

Figure ...........................................2

Fixing at the pump head ..............8

Connections

Figure ...........................................3

Technical specifications ....... 36 ff

Connectors for capillaries ................9

Contents ............................................I

Control characters..........................31

Control inputs .......................... 33, 34

Control interface 13

Figure ...........................................3

Functions....................................33

Control outputs ..............................34

Correction factor.............................22

Current Loop

selector 16

Figure.....................................3

Current Loop interface 18

Figure ...........................................3

Danger..............................................5

Data transmission protocol............31

Deaerating the pump .....................17

Declaration of Conformity ..............46

Degassing of the eluent .................23

Degree of protection ................. 6, 38

<Delete> ........................................21

Dimensions ....................................38

Drift .......................................... 23, 30

Earthing ..................................... 6, 16

Electrical connection ......................13

Electrical safety ................................6

Electromagnetic compatibility .......38

Eluent container .............................12

Eluents............................................23

EMC................................................38

Emitted interference .......................38

ENABLED ........................................21

Evaporation ............................. 23, 30

External control

selector 16

Figure.....................................3

Fastening screws 9

Detach pump head ....................25

Figure ...........................................2

Mounting the pump head ............8

Faults....................................... 23, 30

Figures

list .................................................II

Filter 22

Ordering designation ................ 41

Filter unit PEEK 24

Connection ................................ 11

Ordering designation ................ 40

Filter unit PEEK 6.2821.120

24 ............................................... 11

Connector.................................. 10

Figure......................................... 10

Filter ........................................... 10

Housing ..................................... 10

Mounting.................................... 10

Flow.......................................... 19, 21

Flow accuracy ................................ 36

Flow constancy ............................. 36

Flow range...................................... 36

Front................................................. 2

Fuse cover 10

Figure........................................... 3

Open.......................................... 15

Fuses

Exchange................................... 16

Technical specifications ............ 37

General information on

maintenance .................................. 24

General safety rules ........................ 6

GLP .......................................... 44, 47

GMP............................................... 47

Handling of solvents........................ 6

Hexagon key (6.2621.030) ...... 25, 39

Housing ......................................... 38

IC Net

no connection............................ 30

Immunity to interference................ 38

Information....................................... 5

Information on the Instructions

for Use ............................................. 4

Inlet valve 35

Cleaning .................................... 28

Components.............................. 29

Figure................................... 27, 29

Ordering designation ................ 42

Replacement ............................. 28

Installation........................................ 7

Installing the filter unit PEEK ......... 10

Instructions for Use

(8.818.1023) .............................. 4, 40

Instrument

Switch on/off.............................. 18

Instrument description .................... 1

Interface

Control 13

Figure ....................................3

Current Loop 18

Figure ....................................3

Recorder 14

Figure ....................................3

RS 232 17

818 IC Pump / 8.818.1023 Instructions for Use

6.6 Index

Figure .................................... 3

Interfaces ....................................... 31

Introduction ..................................... 1

IQ ................................................... 44

ISO 9100........................................ 46

Leakage ....................................... 30

Leaks ........................................... 6, 7

Links............................................... 21

List of figures ....................................II

List of program instructions .......... 21

Location ........................................... 7

Mains cable ................................... 16

Ordering designation ................ 39

Mains connection ...................... 6, 15

Technical specifications ............ 37

Mains connection plug 10

Mains connection ...................... 16

Mains connector 10

Figure........................................... 3

Mains frequency ............................ 37

Mains pilot lamp 1

Figure........................................... 2

Operating display ................ 16, 18

Mains switch 10

Figure........................................... 3

Switching the instrument

on/off ................................... 16, 18

Mains voltage

Setting........................................ 15

Technical specifications ............ 37

Mains voltage selector 11

Figure........................................... 3

Setting the mains voltage ......... 15

Maintenance ............................ 23, 24

Malfunctions .................................. 30

Materials ........................................ 38

Maximum pressure limit ................ 36

Metrohm service............................ 24

Microfiltration ................................. 23

Min/Max pressure........................... 19

Minimum pressure limit ................. 36

Mounting the pump head ............... 8

New flow......................................... 19

No connection ............................... 30

Noise.............................................. 30

Notation ........................................... 5

Notes ............................................. 23

Opening the instrument .................. 6

Operation

Via .............................................. 18

Optional accessories .................... 41

OQ ................................................. 44

Ordering designations .................. 39

Organization .................................... 4

Outlet capillary 5

Figure........................................... 2

Deaerating the pump ................ 17

Outlet valve 36

Cleaning..................................... 28

Components .............................. 29

Figure................................... 27, 29

Ordering designations............... 42

Replacement ............................. 28

Packaging ........................................ 7

Parts and controls ........................... 2

Connection ................................ 14

PEEK capillary (6.1831.010)...... 9, 39

PEEK capillary 19

6.1831.010 ....................... 9, 10, 11

PEEK capillary 3

6.1831.010 ................................. 11

PEEK compression fitting (6.2744.070)

Ordering designation................. 41

Pictograms....................................... 5

Piston 27

Cleaning..................................... 25

Figure......................................... 27

Replacement ............................. 25

Piston cartridege 30

Figure......................................... 27

Tighten ....................................... 28

Piston guide sleeve 31

Figure......................................... 27

Piston guide sleeve 33

Figure......................................... 27

Piston seal 34

Figure......................................... 27

Ordering designations............... 41

Replacement ............................. 25

Pmax............................................... 21

Pmin ...............................................21

Power consumption....................... 37

Practical notes ...............................23

Precipitates ....................................23

Pressure ................................... 19, 36

Pressure drop ................................ 30

Pressure measurement...................36

Pressure range ..........................8, 36

Pressure rise .................................. 30

Program .........................................20

818 time program ...................... 20

Protection against foreign

particles ......................................... 23

Protection class .........................6, 38

Pulsation ........................................30

Pulsation dampener

Connection .................................. 9

Pulsation dampener 25

Connection ................................ 11

Ordering designation................. 41

Practical notes ...........................23

Pump.............................................. 21

Icon ............................................18

Pump capacity............................... 36

Pump head 8

Components .............................. 26

Disassemble .............................. 25

Figure..................................... 2, 27

Maintenance ..............................25

Mounting................................ 8, 28

Ordering designation................. 40

Technical specifications ............37

Pump stopped ............................... 34

Purge valve 2

Connection to the pump head ....8

Deaerating the pump.................17

Figure ...........................................2

QA ..................................................44

Quality assurance ..........................44

Quality Management .....................47

Rear..................................................3

Recorder output 14

Figure .......................................... 3

Recorder output socket 14

Technical data ...........................36

Recycling........................................23

Reduced flow rate ..........................33

Remote control ..............................37

Residual pulsation .........................36

RS 232

selector 16

Figure.....................................3

settings....................................... 17

RS 232 interface 17

Baud rate....................................31

Data transmission protocol .......31

Figure ...........................................3

Pin assignment ..........................32

Technical specifications ............37

Safety notes .....................................6

Safety rules ......................................6

Safety shutdown ............................36

Safety specifications ...................... 38

Sapphire sleeve 43

Figure ......................................... 29

Sapphire sphere 44

Figure ......................................... 29

Sapphire supporting ring 32

Figure ......................................... 27

Ordering designation ................. 42

Scope of delivery ...........................39

Screw 26

Figure ......................................... 27

Tighten .......................................28

Screw holder 37

Figure ......................................... 27

Tighten .......................................28

Sealing 46

Figure ......................................... 29

Sealing ring 41

Figure ......................................... 29

Serial number 12

Figure ...........................................3

Service............................................24

Servicing.........................................24

<Set>.......................................20, 22

Setting up the instrument ................ 7

Settings ....................................18, 19

Sleeve 42

Figure ......................................... 29

Software installation ....................... 17

SOP ................................................44

Special tool (6.2617.020)

Dismantle valve..........................28

818 IC Pump / 8.818.1023 Instructions for Use

6 Appendix

Ordering designation ................. 42

Special tool 38

Figure ......................................... 27

Ordering designation ................. 39

Remove piston seal 34.............. 25

Special tool 39

Figure ......................................... 27

Install piston seal 34 .................. 27

Ordering designation ................. 39

Spilled chemicals...........................24

Spring 29

6.2824.050

Figure...................................27

6.2824.060

Figure...................................27

Ordering designation ................. 42

Spring retainer 28

Figure ......................................... 27

Standard operating procedures ....44

<Start> ..........................................20

Static charges ..................................6

Stirring of the eluent.......................23

Stop pump .....................................34

<Stop>...........................................20

Storage ...........................................38

Sunlight.............................................7

Switch

External control 16

Figure.....................................3

Time Constant 15

Figure.....................................3

Switch instrument on/off ......... 16, 18

Syringe (6.2816.020)......................17

Ordering designation .................40

Technical specifications.................36

Time Constant

switch 15

Figure.....................................3

Transport ..........................................7

Transport damage .........................45

Transport safeguard block ..............8

Treatment of Eluents ......................23

Tubing connections

Connection to eluent container. 12

Connection to injection valve.... 11

Tubule (6.2816.040)

Ordering designation ................ 40

Validation ....................................... 44

Valve housing 40

Figure......................................... 29

<Verify> ........................................ 21

Warning............................................ 5

Warranty......................................... 45

Zircon piston (6.2824.070) ...... 25, 41

818 IC Pump / 8.818.1023 Instructions for Use

Metrohm 818IC User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

1.1 Instrument description

1 Introduction

1.2 Parts and controls

1.3 Information about the Instructions for Use

1.4 Safety notes

2 Installation

2.1 Setting up the instrument

2.2 Mounting the pump head

2.3 Attaching the accessories

2.4 Connecting the tubing

2.5 Electrical connection

2.6 Mains connection

2.7 Software installation

2.8 Deaerating the pump

3 Operation

3.1 Switch instrument on/off

3.2 818 IC Pump icon

3.3 Settings in the "818 IC Pump" window

4 Notes – Maintenance – Faults

4.1 Practical notes

4.2 Maintenance and servicing

4.3 Faults and malfunctions

5 Interfaces

5.1 RS 232 interface

5.2 Control interface

6 Appendix

6.1 Technical specifications

6.2 Scope of delivery

6.3 Optional accessories

6.4 Validation / GLP

6.5 Warranty and Conformity

6.6 Index