Olympus BX41 User Manual

INSTRUCTIONS

BX41

SYSTEM MICROSCOPE

This instruction manual is for the Olympus System Microscope Model BX41. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.

This publication is printed on 100% recycled paper		A X 7 4 7 4

BX41

CONTENTS

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 7, “ASSEMBLY” (pages 25 to 27) carefully.

IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-3

1NOMENCLATURE

2 TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

3USING THE CONTROLS

4

5-6

7-18

3-1	Base.....................................................................................................................................................................................................................					7-8
	1	Voltage Indication; 2	Using the Light Intensity Preset Switch; 3 Using the Filters
3-2	Focusing Block ..........................................................................................................................................................................................					9
	1 Replacing the Fine Adjustment Knob;				2 Adjusting the Coarse Adjustment Knob Tension;
	3	Pre-focusing Lever
3-3	Stage...............................................................................................................................					............................................................................ 10-12
	1	Placing the Specimen;	2	Adjusting the X- and Y-Axis Knob Tension;
	3	Rotating the Stage;	4	Adjusting the Stage Height
3-4	Observation Tube...............................................................................................................................					...................................... 13-15
	1	Adjusting the Interpupillar Distance;			2	Adjusting the Diopter;
	3	Using the Eye Shades;			4 Using Eyepiece Micrometer Disks;
	5	Selecting the Light Path;			6	Adjusting the Tilt
3-5	Condenser...........................................................................................................................................................................................					16-17
	1	Centering the Condenser;		2 Compatibility of Objectives and Condensers
3-6	Immersion Objectives...............................................................................................................................					................................... 18
	1	Using Immersion Objectives
3-7	Objectives with Correction Collar				..............................................................................................................................	18

4TROUBLESHOOTING GUIDE

5SPECIFICATIONS

6OPTICAL CHARACTERISTICS

19-21

22-23

24

7ASSEMBLY — See this section for the replacement of the light bulb. — 25-27

......................................................................PROPER SELECTION OF THE POWER SUPPLY CORD

28-29

IMPORTANT

This microscope employs a UIS2/UIS (Universal Infinity System) optical design, and should be used only with UIS2/UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series and objectives/eyepieces for the UIS series are also usable. For details, please consult Olympus or the catalogue.) Less than optimum performance may result if inappropriate accessories are used.

SAFETY PRECAUTIONS

(Fig. 1)

1.After the equipment has been used in an observation of a specimen that is accompanied with a potential of infection, clean the parts coming in

@contact with the specimen to prevent infection.

· Moving this product is accompanied with the risk of dropping the specimen. Be sure to remove the specimen before moving this product.

· In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures.

2. Install the microscope on a sturdy, level table or bench so as not to block the air vents on the underside of the base.

Fig. 1

Do not place the microscope on a flexible surface, as this could result in blocking the air vents and cause overheating or a fire.

3.To prevent obstruction of the natural convection-based air cooling of the microscope, make sure to leave at least 10 cm of free space between walls or other objects, and the left, right and rear sides of the microscope and the lamp socket when installing the microscope.

4.To avoid potential shock hazards and burns when replacing the light bulb, set the main switch @ to “” (OFF) then disconnect the power cord from the wall outlet in advance. Whenever you replace the bulb

during use or right after use, allow the lamp socket ² and bulb to cool before touching. (Figs. 1 & 2)

Designated bulb	6V30WHAL (PHILIPS 5761)

#The microscope also incorporate a fuse (this should be replaced by the manufacturer or authorized agent).

5.Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section

		“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
²		this instruction manual. If the proper power cord is not used, product
		safety and performance cannot be guaranteed.
		6. Always ensure that the grounding terminal of the microscope and that
		of the wall outlet are properly connected. If the equipment is not
		grounded/earthed, Olympus can no longer warrant the electrical safety
	Fig. 2	and performance of the equipment.

7.Never insert metallic objects into the air vents of the microscope frame as this could result in electrical shock, personal injury and equipment damage.

8.The power cord may be melt by the heat of lamp socket if the cord comes in contact with it. Distribute the power cord at an enough distance from the lamp socket.

1

BX41

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner.

Symbol	Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or damage to the equipment.

lIndicates that the main switch is ON.

Indicates that the main switch is OFF.

Warnings

Warning engraving/stickers are placed at parts where special precaution is required when handling and using the microscope. Always heed the warnings.

Warning engraving	Lamp socket
position	(Warning against high temperature)
Warning sticker	Microscope frame rear panel
position	(Warning against high temperature)

Should warning stickers become soiled, peeled off, etc., contact Olympus for replacement.

1Getting Ready

@

²

Fig. 3

1.A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.

2.Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating conditions, refer to section 5, “SPECIFICATIONS”.)

3.When moving the microscope, remove the specimen and modules that may drop during transport and carefully carry it with the grasping part on the rear of the arm and the base as shown in Fig. 3 (Weight: approx. 13 kg). Be sure to remove the specimen @ that is likely to drop and the filter ²

on the filter mount.

#If an intermediate attachment hinders you grasp the grasping part on the rear, remove the intermediate attachment before carrying.

#Damage to the microscope will occur if you grasp it by the stage, coarse/fine adjustment knob or binocular section of the observation tube.

4.The BX41 can be used with up to two intermediate attachments (e.g. a U- CA magnification changer, U-EPA2 eyepoint adjuster, etc.). For restrictions when using two intermediate attachments, make sure to read the in-

struction manual provided with the respective intermediate attachments.

#The microscope becomes unstable particularly when the U-TRU trinocular attachment is attached on the side position or the microscope height is increased. In this case, take measures for preventing the microscope from toppling down.

2

2Maintenance and Storage

1.To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.

Since the absolute alcohol is highly flammable, it must be handled carefully.

Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical equipment that is being switched on or off.

Also remember to always use it only in a well-ventilated room.

2.The filter mount lens on the base is made of plastic. Do not rub it with a strong force to prevent damaging it.

3.Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

4.Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.

5.When the microscope is not in use, be sure to set the main switch to “” (OFF). After checking that the lamp housing has been cooled down, place the dust-prevention cover on the microscope for storage.

6.When disposing of the microscope. Check the regulations and rules of your local government and be sure to observe them.

3Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment. } : Indicates commentary (for ease of operation and maintenance).

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection

against harmful interference when the equipment is operated in a commercial environment. This

equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in

accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case

the user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance

could void the user’s authority to operate the equipment.

3

BX41

1 NOMENCLATURE

} If you have not yet assembled the microscope, read section 7, “ASSEMBLY” (pages 25 to 27).

Interpupillary distance adjustment scale (Page 13)

Diopter adjustment ring (Page 13)

Slide holder (Page 10)

Aperture iris diaphragm ring (Page 17)

Fine adjustment knob (Page 9)

(Detachable)

Pre-focusing Lever (Page 9)

Filter mount (Page 7)

Field iris diaphragm ring (Page 16)

Brightness adjustment knob (Page 7) (Lamp voltage adjustment knob)

Allen screwdriver

(accommodation position)

Condenser height adjustment knob (Page 16)

Main switch (Page 1)

Voltage indicator LED

Light intensity preset switch (Page 7)

Light intensity preset adjustment screw (Page 7)

Y-axis knob (Page 11)

X-axis knob (Page 11)

Coarse adjustment knob (Page 9)

Coarse adjustment tension adjustment ring (Page 9)

4

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

Set the main switch to “ I ” (ON) and adjust the brightness.

Select the light path (trinocular tube).

Place the specimen on the stage.

Engage the 10X objective in the light path.

Bring the specimen in focus.

Adjust the interpupillary distance.

Adjust the diopter.

Adjust the light axis.

Adjust the aperture iris and field iris diaphragms.

Engage the desired objective in the light path and bring the specimen in focus.

Insert the required filters.

Adjust the brightness.

(Controls Used)	(Page)
@Main switch
²Brightness adjustment knob	(P. 7)

³Light path selector knob	(P. 14)
|Slide holder	(P. 10)
ƒX/Y-axis knobs	(P. 11)
…Revolving nosepiece
†Coarse/fine adjustment knobs	(P. 9)
‡Binocular tube	(P. 13)
ŠDiopter adjustment ring	(P. 13)
‰Condenser height adjustment	(P. 16)
knob
‹Condenser centering knob	(P. 16)
ŒAperture iris diaphragm ring	(P. 17)
™Field iris diaphragm ring	(P. 16)
…Revolving nosepiece
šFilter mount	(P. 7)
²Brightness adjustment knob	(P. 7)

Start observation.

5

BX41

‡

³ (Trinocular observation tube only)

|

Š

…

@

‰

Œ

ƒ

‹

†

™

š

²

†

} Copy the observation procedure pages on separate sheets and post it near your microscope.

6

USING THE CONTROLS

3-1 Base

1

Voltage Indication

(Fig. 4)

1.Turn the brightness adjustment knob @ clockwise to increase the voltage and make illumination brighter.

2.The numerals ² below the knob indicate the approximate voltage.

²@

Fig. 4

2

Using the Light Intensity Preset Switch

(Fig. 5)

@

²

Fig. 5

}The light intensity preset switch @ makes it possible to limit the light intensity to a preselected level regardless of the position of the brightness adjustment knob. The light intensity preset switch has been set to about 4 V at the factory.

1.Press the light intensity preset switch @ to the ON position. (The face of the switch lights when it is ON.)

2.Using a small flat-blade screwdriver, turn the preset adjustment screw ² to obtain the required light intensity. Turning the screw clockwise increases brightness.

3.When the light intensity preset switch is set to OFF, the brightness returns to the level set by the brightness adjustment knob.

}While the light intensity preset switch is ON, turning the brightness adjustment knob does not affect brightness.

3

Using the Filters

(Figs. 6 - 10)

}You can place a filter in the light path with either method.

· Place a filter on the filter mount on the base and engage in the light path. (Page 7)

·Insert a filter in the U-FC filter cassette, attach it on the filter mount and engage it in the light path by sliding the filter level. (Page 8)

@	Mounting a Single Filter	(Fig. 6)

A filter with a diameter of 45 mm can be placed on the filter mount on the base. If you need to place multiple filters, please purchase the U-FC filter cassette.

#Even when a filter cassette is used, a filter with thickness of no more than 3 mm can still be placed on the filter mount.

Fig. 6

7

BX41

@

Fig. 7

³

²

|

ƒ

Fig. 8

Fig. 9

Using the Filter Cassette	(Figs. 7 - 10)

Loading Filters into Filter Cassette

}The filter cassette accommodates filters with a diameter of 45 mm and thickness of 2.7 mm or less.

}The filter cassette has two filter levels on the right side and one on the left side.

1.Move all filter levers to the OUT position except for the one belonging to the slot into which the filter is to be inserted.

2.Slide lever @ to the IN position. Make sure that it clicks securely into place. (Fig. 7)

3.Holding the lever in the position shown in Fig. 7, put the filter into the cassette by inserting it in the direction indicated by the arrow.

4.Insert the other two filters in the same manner.

Mounting the Filter Cassette

1.Fully loosen the filter cassette clamping screw ². (Fig. 8)

2.Align the key ³ on the bottom surface of the filter cassette with the positioning slot | on the filter mount, then snap the filter cassette into place from above.

3.Rotate the filter cassette to align its sides with the base. (Fig. 9)

4.Align the clamping screw ² with the positioning hole ƒ on the filter mount, then tighten the screw to fasten the filter cassette.

#When the filter cassette is installed, the condenser may hit it when the stage is lowered. Therefore, exercise caution when lowering the stage with the filter cassette installed.

Using the Filter Cassette	(Fig. 10)
Usable Filters				Applications
45LBD-IF*		Color balancing filter
45ND-6, 45ND-25		Neutral density filter
45G-530, 45G-533, 45IF550*		Green
45Y-48		Yellow			B&W contrast filter
45O-560		Orange

Up to three of the above filters can be loaded in the filter cassette. Moving the levers … on the left and right sides of the cassette to the IN position brings the corresponding filter into the light path.

*When an IF (Interference) filter is used, flare or ghost may be observed. The flare or ghost may be reduced by additionally using an ND filter with the IF filter.

…

Fig. 10

8