Metrohm 820IC User Manual
819 IC Detector
820 IC Separation Center
5.819.0010 Program
CH-9101 Herisau/Switzerland
E-Mail info@metrohm.com
Internet www.metrohm.com
Instructions for Use
8.819.1013
CH-9101 Herisau/Switzerland
E-Mail info@metrohm.com
Internet www.metrohm.com
819 IC Detector
820 IC Separation Center
5.819.0010 Program
Instructions for Use
8.819.1013 05.2004 / pkl
Teachware
Metrohm AG
Oberdorfstrasse 68
CH-9101 Herisau
teachware@metrohm.com
2nd Edition 2004
These instructions are protected by copyright. All rights reserved.
Although all the information given in these instructions has been checked with great care, errors
cannot be entirely excluded. Should you notice any mistakes please inform the author at the
address given above.
Table of contents
Table of contents
1 Introduction.................................................... 1
1.1 Instrument description ............................................................. 1
1.1.1 819 IC Detector .............................................................................1
1.1.2 820 IC Separation Center..............................................................2
1.1.3 Schematic arrangement of the IC systems...................................3
1.2 Parts and controls .................................................................... 4
1.2.1 819 IC Detector .............................................................................4
1.2.2 820 IC Separation Center..............................................................6
1.3 Information on the Instructions for Use ................................ 10
1.3.1 Organization ................................................................................10
1.3.2 Notation and pictograms ............................................................11
1.4 Safety notes ............................................................................12
1.4.1 General precautionary rules ........................................................ 12
2 Installation ................................................... 13
2.1 Setting up the instrument ....................................................... 13
2.1.1 Packaging.................................................................................... 13
2.1.2 Check...........................................................................................13
2.1.3 Location ....................................................................................... 13
2.1.4 Arrangement of the instruments..................................................13
2.2 Installation of accessories .....................................................15
2.2.1 Insert detector block.................................................................... 15
2.2.2 Connection of column heating....................................................18
2.2.3 Connection of syringe and aspirating tubing..............................19
2.2.4 Connection of the drain tube.......................................................19
2.2.5 Connection of the 6.5324.000 Bottle rack (option).....................19
2.3 Electrical connection .............................................................. 20
2.3.1 Connection to 830 IC Interface ...................................................20
2.3.2 Connection to PC ........................................................................23
2.4 Mains connection.................................................................... 25
2.4.1 819 IC Detector ...........................................................................25
2.4.2 820 IC Separation Center............................................................27
2.4.3 Column heating ...........................................................................27
2.5 Software-installation............................................................... 27
2.6 Capillary connections............................................................. 28
2.7 Connection of 818 IC Pump ................................................... 29
2.7.1 Electrical connection ...................................................................29
2.7.2 Pulsation dampener ....................................................................29
2.7.3 Filter unit PEEK............................................................................30
2.7.4 Connection to injection valve ......................................................31
2.8 Precolumns ............................................................................. 33
2.9 Separating columns and suppressor module ....................... 34
2.9.1 General information on separating columns ..............................34
2.9.2 Installing a separating column in the column heating ................35
2.9.3 Selection of the sample loop ......................................................37
2.9.4 General information on suppressor module ............................... 37
2.9.5 One-channel system without suppressor module......................39
2.9.6 Two-channel system without suppressor module ...................... 41
2.9.7 One-channel system with suppressor module ........................... 42
2.9.8 Two-channel system with suppressor module ...........................47
2.9.9 Leak testing and conditioning.....................................................48
819 IC Detector / 820 IC Separation Center
I
Table of contents
3 Operation ...................................................... 50
3.1 819 IC Detector .......................................................................50
3.1.1 819 IC Detector icon ...................................................................50
3.1.2 Settings in the "819 IC Detector" window.................................... 50
3.2 820 IC Separation Center ....................................................... 60
3.2.1 820 IC Separation Center icon.................................................... 60
3.2.2 "820 IC Separation Center" window ............................................ 60
4 Notes – Maintenance – Faults ...................... 66
4.1 Practical notes on ion chromatography ................................66
4.1.1 Separating columns .................................................................... 66
4.1.2 Pumps ......................................................................................... 67
4.1.3 Eluents......................................................................................... 68
4.1.4 Suppressor module..................................................................... 69
4.1.5 Connections ................................................................................ 69
4.2 Maintenance and servicing ....................................................69
4.2.1 General information..................................................................... 69
4.2.2 Passivation .................................................................................. 70
4.2.3 Recycling..................................................................................... 70
4.2.4 Shutdown ....................................................................................70
4.2.5 Changing separating columns ................................................... 71
4.2.6 Regeneration of suppressor .......................................................72
4.2.7 Cleaning the suppressor............................................................. 73
4.2.8 Replacement of suppressor module .......................................... 75
4.3 Faults and malfunctions ......................................................... 77
4.3.1 Malfunctions and their rectification ............................................. 77
5 Interfaces ..................................................... 79
5.1 RS 232 interface...................................................................... 79
5.1.1 Data transmission protocol......................................................... 79
5.1.2 Pin assignment............................................................................ 80
5.2 Remote interfaces................................................................... 81
5.2.1 "Remote" interface ....................................................................... 81
5.2.2 "IC Separation Center" interface.................................................. 83
5.3 Analog output.......................................................................... 85
5.4 External power supply for 820 IC Separation Center .......... 85
5.5 Valve interfaces....................................................................... 86
6 Appendix ....................................................... 87
6.1 Technical data......................................................................... 87
6.1.1 819 IC Detector ........................................................................... 87
6.1.2 820 IC Separation Center............................................................ 90
6.2 Scope of delivery .................................................................... 92
6.2.1 819 IC Detector ........................................................................... 92
6.2.2 820 IC Separation Center............................................................ 94
6.3 Optional accessories ............................................................ 103
6.3.1 Accessories for 820 IC Separation Center ...............................103
6.3.2 Column heating......................................................................... 104
6.3.3 Cable ......................................................................................... 105
6.3.4 Literature.................................................................................... 105
6.4 Validation / GLP ....................................................................106
819 IC Detector / 820 IC Separation Center
II
Table of contents
6.5 Warranty and Conformity ..................................................... 107
6.5.1 Warranty.....................................................................................107
6.5.2 Declaration of Conformity .........................................................108
6.5.3 Declaration of Conformity .........................................................109
6.5.4 Quality Management Principles ................................................110
6.6 Index ...................................................................................... 111
819 IC Detector / 820 IC Separation Center
III
Table of contents
List of figures
Fig. 1: Block diagram of the ion chromatography systems ................................. 3
Fig. 2: Front of the 819 IC Detector ...................................................................... 4
Fig. 3: Rear of the 819 IC Detector....................................................................... 5
Fig. 4: Front of the 820 IC Separation Center....................................................... 6
Fig. 5: Rear of the 820 IC Separation Center ....................................................... 7
Fig. 6: Column heating closed ............................................................................. 9
Fig. 7: Insert detector block in the Separation Center........................................ 15
Fig. 8: Connection Detector 819 – 2.820.0X10/2.820.0X30 ............................... 16
Fig. 9: Connection 2 x 819 Detector – 2.820.0X20............................................. 17
Fig. 10: Install column heating in Separation Center ........................................... 18
Fig. 11: Connection 820 – column heating .......................................................... 19
Fig. 12: Connection of 820 via 819 to 830............................................................ 20
Fig. 13: Connection of 819 and 820 to 830 .......................................................... 22
Fig. 14: Connection of 819 and 820 to a PC ........................................................ 24
Fig. 15: Setting the mains voltage........................................................................ 26
Fig. 16: Connect capillaries.................................................................................. 28
Fig. 17: Connection of 818 IC Pump to 830 IC Interface...................................... 29
Fig. 18: Filter unit PEEK (6.2821.120)................................................................... 30
Fig. 19: Connection to injection valve .................................................................. 31
Fig. 20: Insert column in column heating ............................................................. 35
Fig. 21: Interior of the 2.820.0310 Separation Center .......................................... 39
Fig. 22: Interior of the2.820.0320 Separation Center ........................................... 41
Fig. 23: Interior of 2.820.0330 Separation Center ................................................ 42
Fig. 24: Suppressor module connections ............................................................ 44
Fig. 25: Interior of 2.820.0320 Separation Center with suppressor module........ 47
Fig. 26: Operating principle of Auto-zero ............................................................. 52
Fig. 27: Diagram of measuring and full-scale range............................................ 54
Fig. 28: Flow path of the injection valve ............................................................... 62
Fig. 29: Assembling the suppressor .................................................................... 74
819 IC Detector / 820 IC Separation Center
IV
1.1 Instrument description
1 Introduction
1.1 Instrument description
1.1.1 819 IC Detector
The 819 IC Detector is a conductivity detector especially designed for
ion chromatography with an extensive operating range and high sensitivity for the recording of chromatograms with and without chemical
suppression. The associated thermostattable detector block is normally
installed in the 819 IC Separation Center, but can also be used as a
separate detector. The two following versions are available:
• 2.819.0010 IC Detector with metal detector block
• 2.819.0110 IC Detector with metal-free detector block
The 819 IC Detector can be fully remotely controlled via the Metrohm
«IC Net» software. All functions can be quickly and easily accessed by
the software and can be clearly shown on the screen. Apart from setting
the measuring parameters, it is possible to draw up any time programs
required. These can be used to control a large number of instrument
functions and freely programmable events.
The 819 IC Detector is connected via the 830 IC Interface or directly to
a PC. The control of the instrument and the recording and evaluation of
the chromatograms take place under «IC Net». It is also possible to
control external devices or start functions in the IC system from these
devices via a “Remote” interface using programmable signals.
819 IC Detector / 820 IC Separation Center
1
1 Introduction
1.1.2 820 IC Separation Center
The 820 IC Separation Center is a thermally and electronically insulated wet-chemistry component that accommodates injectors, columns,
column heating, detector blocks, suppressor module, pulsation dampeners and various optional sample preparation modules. The 820 IC
Separation Center is also remotely controlled via the Metrohm «IC Net»
software; it can either be connected to the 819 IC Detector and controlled through it, or connected directly to the 830 IC Interface and controlled directly from «IC Net».
The following versions are available:
• 2.820.0210 IC Separation Center with 1 injector for a one-channel
system with column heating, metal-free
• 2.820.0220 IC Separation Center with 2 injectors for a
two-channel system with column heating, metal-free
• 2.820.0230 IC Separation Center with 1 injector and 1 Metrohm
Suppressor Module MSM for a one-channel system
with column heating, metal-free
• 2.820.0310 IC Separation Center with 1 injector for a one-channel
system, metal-free
• 2.820.0320 IC Separation Center with 2 injectors for a
two-channel system, metal-free
• 2.820.0330 IC Separation Center with 1 injector and 1 Metrohm
Suppressor Module MSM for a one-channel system;
metal-free
2
819 IC Detector / 820 IC Separation Center
1.1 Instrument description
1.1.3 Schematic arrangement of the IC systems
The 819 IC Detector and 820 IC Separation Center are the main components of a modular ion chromatography system that can be expanded to meet the wishes of the individual user (see Fig. 1). The
minimum configuration of the one-channel system also includes a 818
IC Pump, a separating column, a 830 IC Interface and an PC. The twochannel system requires at least a second 819 IC Detector and a second 818 IC Pump. Both systems can be extended virtually without any
limits by further modular Metrohm IC devices such as sample changers
for automation, sample preparation devices, components for postcolumn derivation and other detectors. Further, practically all HPLC peripherals and parts available on the market such as precolumns, additional separating columns, additional detectors and other injection systems can be seamlessly integrated in the system.
However, the individual IC units can also be freely combined with
common HPLC instruments. This offers the possibility of expanding
your system to a stand-alone ion chromatograph.
Suppressor
Sample
changer
Sample
preparation
Two-channel systemOne-channel system
PR
PC
830
819
D
D
M
M
820
C
C
S
W
I
I
818 818
WW
S
W
EE
819
Suppressor
Sample
changer
Sample
preparation
Fig. 1: Block diagram of the ion chromatography systems
C Separating Column M
D Detector PC PC 819 IC Detector
E Eluent PR Printer 820 IC Separation Center
I Injector S Probe
IF Interface W Waste
819 IC Detector / 820 IC Separation Center
Suppressor module
818 IC Pump
3
1 Introduction
1.2 Parts and controls
In this Section you will find the numbers and designations of the parts
and controls of the 819 IC Detector and 820 IC Separation Center.
The numbering applies throughout the instructions for use, i.e. bold
numbers in the text (e.g.
here.
1.2.1 819 IC Detector
1
3
) refer to the parts and controls illustrated
Fig. 2: Front of the 819 IC Detector
Mains pilot lamp
1
Lights up when instrument is switched on
4
819 IC Detector / 820 IC Separation Center
1.2 Parts and controls
2 3 5 4
Fig. 3: Rear of the 819 IC Detector
Mains switch
2
For switching instrument on/off:
I = ON 0 = OFF
6
7
8 9 10
Connection for detector block
6
Model plate with serial number
7
Mains connection plug
3
Mains connection see Section 2.4.1
Fuse holder
4
Changing the fuses, see Section 2.4.1
Analog output
5
0...1 V
Remote interface
8
remote I/O lines for connection of external devices
RS 232 interface for connection of
9
IC Interface 830 or PC
Connection for 820 IC Separation
10
Center
819 IC Detector / 820 IC Separation Center
5
1 Introduction
1.2.2 820 IC Separation Center
1
12
Fig. 4: Front of the 820 IC Separation Center
Mains pilot lamp
11
Lights up when instrument is switched
on
Thermostat lamp
12
Lights up when column heating is
Connection for syringe 6.2816.020
13
Feedthrough
14
for sample, eluent, suppressor reagent,…
connected, see Section 2.2.2 and Sec-
tion 2.4.3
6
819 IC Detector / 820 IC Separation Center
13
14
1.2 Parts and controls
21
22
24
15
15 16 18 20
17 19
15
23
15
25
15
26
27
14
28
29
30
15
31
14
15
Fig. 5: Rear of the 820 IC Separation Center
819 IC Detector / 820 IC Separation Center
32 18
33 34
18
15
7
1 Introduction
Feedthrough
14
for sample, eluent, suppressor reagent,…
Knurled screw
15
for fastening rear panel 21, resp. 28.
Opening for detector cable B
16
opening for connecting cable:
detector block B – 819
Opening for outlet capillary B
17
discharge of the eluent of column B to
waste
Rear panel opening
18
(closed with plastic stopper) for additional supply and discharge lines to
and from the inner compartment
Opening for outlet capillary A
19
discharge of the eluent of column A to
waste
Opening for detector cable A and
20
for connecting cable to the column
heating
opening for connecting cable:
detector block B – 819
column heating – 820 (6.2108.120)
Detachable rear panel
21
access to top part of the inner compartment
RS 232 interface for column heat-
22
ing
to connect to 830 IC Interface or PC
with cable 6.2134.040
Connection for external power
25
supply of the Separation Center
connection of power supply unit
6.2152.000
(5 V, 0.5 A / 24 V, 2 A) in operation
without 819 IC Detector
Terminal block for valve B
26
Ground, Fill, Inject:
inputs for control of the valve
RUN Pos. / COM Fill:
output signal on switching of the
valve to position "FILL"
RUN Integr. / COM Start:
output signal on switching of the
valve to position "INJECT"
Connection for 819 IC Detector
27
Detachable rear panel
28
access to bottom part of the inner
compartment
Model plate with serial number
29
Connection for 819 IC Detector A
30
Terminal block for Valve A
31
Ground, Fill, Inject:
inputs for control of the valve
RUN Pos. / COM Fill:
output signal on switching of the
valve to position "FILL"
RUN Integr. / COM Start:
Output signal on switching of the
valve to position "INJECT"
Opening for inlet capillary B
32
supply of the eluent for column B
Connection for column heating
23
with cable 6.2108.120
Connection for drain tube
33
for discharge of spilled liquid from the
inner compartment
Connection for external power
24
supply of column heating
with power supply unit 6.2152010
Opening for inlet capillary A
34
supply of the eluent for column A
8
819 IC Detector / 820 IC Separation Center
1.2 Parts and controls
Column heating
35
36
38
39
37
39
36
Fig. 6: Column heating closed
Feedthrough
35
for the capillary from the separating
column to detector
Knurled screw
36
for fastening the heater cover 37
40
41
Front Rear
Magnetic holder
39
for fastening the column heater in the
inner compartment of the Separation
Center
Model plate with serial number
40
Cover of column heating
37
Connection for Separation Center
38
with cable 6.2108.120
819 IC Detector / 820 IC Separation Center
Feedthrough
41
for column connection capillary 50
9
1 Introduction
1.3 Information on the Instructions for Use
Please read through these Instructions for Use carefully before you put
the 819 IC Detector and 820 IC Separation Center into operation. The
Instructions for Use contain information and warnings which must be
heeded the user to assure safe operation of the instruments.
1.3.1 Organization
These 8.819.1013 Instructions for Use for the 819 IC Detector and
820 IC Separation Center provide a comprehensive overview of the installation, startup procedure, operation, fault rectification and technical
specifications of these instruments. The Instructions for Use are organized as follows:
Section 1 Introduction
General description of instruments, parts and controls
and safety notes
Section 2 Installation
Installation of 819 IC Detector / 820 IC Separation
Center, electrical connections, tubing connections,
mains connection
Section 3 Operation
Operation via «IC Net»
Section 4 Notes - Maintenance - Faults
Practical notes, maintenance, fault rectification
Section 5 Interfaces
Description of the interfaces
Section 6 Appendix
Technical data, standard equipment, options, validation, warranty, declaration of conformity, index
To find the information you require about the instrument please use either the Table of contents or the Index at the back.
As an addition to the Instructions for Use the Metrohm Monographs
listed in Section 6.3.4 can be requested on the Internet under
http://www.metrohm.com
your local Metrohm agency. Detailed information about separating columns available from Metrohm can be found in the Metrohm IC Col-
umn Catalog or on the Internet under http://www.metrohm.com
ion chromatography range of products. Information about special IC
applications is given in the relevant "Application Bulletins" or "Appli-
cation Notes"; these can be found on the Internet under
http://www.metrohm.com
quested free of charge from your local Metrohm agency.
in the literature section, or free of charge from
in the
in the applications section or can be re-
10
819 IC Detector / 820 IC Separation Center
1.3 Information on the Instructions for Use
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these Instructions for Use:
Range Menu item, parameter or entry
value
in «IC Net» program
SYSTEM STATE Program window
in «IC Net» program
<OK> Button
in «IC Net» program
7 Part or control of 819 / 820
3 Part or control of 818
21 Part or control of 833
Danger/Warning
This symbol indicates a possible
risk of death or injury to the user
and possible damage to the
instrument or its components by
electricity.
Danger/Warning
This symbol indicates a possible
risk of death or injury to the user
and possible damage to the instrument or its components.
Attention
This symbol indicates important information that you should read before continuing.
Information
This symbol indicates additional information and tips which may be of
particular use to you.
819 IC Detector / 820 IC Separation Center
11
1 Introduction
1.4 Safety notes
While electrical safety in the handling of the 819 IC Detector and 820 IC
Separation Center is assured in the context of the specifications EN/IEC
61010-1 / UL 3101-1 (protection class I), the following points should be
noted:
• Mains connection
Setting of the mains voltage, checking the mains fuse and the
mains connection must be effected in accordance with the instruc-
tions in Section 2.4.
• Opening the 819 IC Detector
Inside the instrument there are no parts which must be set or adjusted
by the user.
If the 819 IC Detector is connected to the mains then the instrument
must not be opened, nor should any components be removed from it
as otherwise you run the risk of coming into contact with currentcarrying components. For this reason you should always separate the
instrument from sources of electricity before opening it and also en-
3
sure that the mains plug is removed from mains connector
!
• Opening the 820 IC Separation Center
The instrument contains no components that can be set or adjusted by
the user.
Disconnect all connecting cables on the rear of the 820 IC
Separation Center before you remove the middle housing panel
with connectors.
• Protection against static charges
Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 819 IC Detector or 820 IC Separation Center, you should
earth yourself and any tools you are using by touching an earthed object (e.g. housing of the instrument or a radiator) to eliminate any
static charges which exist.
1.4.1 General precautionary rules
• Handling of solvents
Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.
12
819 IC Detector / 820 IC Separation Center
2.1 Setting up the instrument
2 Installation
2.1 Setting up the instrument
2.1.1 Packaging
819 IC Detector and 820 IC Separation Center are supplied together
with the separately packed accessories in special packagings
containing shock-absorbing foam linings designed to provide excellent
protection. The actual instrument is packed in an evacuated
polyethylene bag to prevent the ingress of dust. Please store all these
special packagings as only they assure transport of the instruments
free from damage.
2.1.2 Check
After receipt, immediately check whether the shipment is complete and
has arrived without damage (compare with delivery note and list of
accessories in Section 6.2). In the case of transport damage, see
instructions in Section 6.5.1 "Warranty".
2.1.3 Location
Position the instrument the laboratory at a location convenient for operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals.
To avoid disturbing temperature influences on the insulated column
compartment, the pump and eluent reservoir must be protected
against direct sunlight.
2.1.4 Arrangement of the instruments
Modular IC instruments can be stacked on top of each other in any sequence.
In a modular system any instruments containing liquid-transporting
assemblies should always be located as low down in the stack as
possible, so that any leaks which may occur in tubing or connections
will cause no damage to the other instruments by escaping liquids
such as acids.
819 IC Detector / 820 IC Separation Center
13
2 Installation
In one-channel operation, the 818 IC Pump, 820 IC Separation Center
and 819 IC Detector are best stacked on top of one another in this order.
In two-channel operation (2.820.0X20 IC Separation Center), the optimum arrangement (1, 2 or 3 towers) depends on the laboratory space
available. However, the 818 IC Pumps should be set up at the very bottom and the 819 IC Detectors at the very top.
To ensure that the arrangement of pumps and detectors for the two
channels A and B is clearly apparent in two-channel operation, it is
advantageous to mark the instruments. The 6.2248.000 Magnetic
plate is enclosed with the 819 IC Detector for this purpose. It can be
cut to the desired size, labeled (e.g. with "A" or "B") and affixed to the
appropriate instrument.
14
819 IC Detector / 820 IC Separation Center
2.2 Installation of accessories
2.2 Installation of accessories
2.2.1 Insert detector block
In order to operate an 820 IC Separation Center with an 819 IC Detector
the detector block contained in the standard equipment of the detector
(1.732.0110 Detector block metal-free or 1.732.0100 Metal) must
be inserted in the upper part of the Separation Center.
Fig. 7: Insert detector block in the Separation Center
2.820.0X10/2.820.0X30 IC Separation Center
The instrument versions 2.820.0X10 and 2.820.0X30 of the IC Separation Center are operated with one
as follows when connecting the two instruments and the detector block:
1 Install detector block
• Unscrew the four knurled screws 15 from the top rear panel
21 of the 820 IC Separation Center and remove rear panel
(see Fig. 5).
• Position detector block from the back in the space provided
in the 820 IC Separation Center on the right and push fully to
the front (see Fig. 7, resp. Fig. 21 and Fig. 23).
• Insert the cable permanently attached to the detector block in
opening 20 and the outlet capillary in opening 19 "Waste A"
of the rear panel 21.
• Replace rear panel 21 and screw to the 820 IC Separation
Center using the four knurled screws 15.
819 IC Detector. It is best to proceed
819 IC Detector / 820 IC Separation Center
15
2 Installation
819
Cable from
detector block
820
Fig. 8: Connection Detector 819 – 2.820.0X10/2.820.0X30
2 Connect detector block
• Plug the gray connecting cable permanently attached to the
detector block into connection 6 „Detector Block“ of the 819
IC Detector and fasten to the instrument by tightening the
screws in the cable connector (see Fig. 8).
3 Connect waste container
• Lead the outlet capillary of the detector block to a sufficiently
large waste container and fix in place.
2.820.0X20 IC Separation Center
The two-channel versions 2.820.0X20 of the IC Separation Center can
be operated with two
819 IC Detectors. It is best to proceed as follows
when connecting the instruments and the two detector blocks:
1 Install detector blocks
• Unscrew the four knurled screws 15 from the top rear panel
21 of the 820 IC Separation Center and remove rear panel
(see Fig. 5).
• Position first detector block from the back in the space pro-
vided in the 820 IC Separation Center on the right and push
fully to the front (see Fig. 7, resp. Fig. 22 and Fig. 25).
• Position second detector block from the back in the space
provided in the 820 IC Separation Center on the left and push
fully to the front (see Fig. 7, resp. Fig. 22 and Fig. 25).
• Insert the cable permanently attached to the detector block A
in opening 20 and the outlet capillary in opening 19 "Waste A"
of the rear panel 21.
• Insert the cable permanently attached to the detector block B
in opening 16 and the outlet capillary in opening 17 "Waste B"
of the rear panel 21.
16
819 IC Detector / 820 IC Separation Center
2.2 Installation of accessories
• Replace rear panel 21 and screw to the 820 IC Separation
Center using the four knurled screws 15.
2 Connect detector blocks
• Plug the gray connecting cable permanently attached to the
detector block A into connection 6 „Detector Block“ of the
first 819 IC Detector and fasten to the instrument by
tightening the screws in the cable connector (see Fig. 9).
• Plug the gray connecting cable permanently attached to the
detector block B into connection 6 „Detector Block“ of the
second 819 IC Detector and fasten to the instrument by
tightening the screws in the cable connector (see Fig. 9).
3 Connect waste container
• Lead the outlet capillary of both detector blocks to a suffi-
ciently large waste container and fix in place.
819
819
Cable from
detector block B
820
Fig. 9: Connection 2 x 819 Detector – 2.820.0X20
Cable from
detector block A
819 IC Detector / 820 IC Separation Center
17
2 Installation
2.2.2 Connection of column heating
The instrument versions 2.820.02X0 of the IC Separation Center contain
a column heating for thermostatting the separating column as an option. If you wish to use this column heating then it should be installed as
follows:
Fig. 10: Install column heating in Separation Center
1 Laying the connection cable
• Screw off the 4 knurled screws 15 from upper rear panel 21
of the 820 IC Separation Center and remove the rear panel
(see Fig. 5).
• Lead 6.2108.120 Cable through opening 20.
• Replace rear panel 21 and screw it onto 820 IC Separation
Center using the 4 knurled screws 15.
2 Insert column heating
• Carefully place the column heating inside the Separation
Center. If the column has already been inserted in the heating
then be careful with the capillaries at feedthroughs 35 and 41
(see Section 2.9.2).
• Use magnetic holder 39 and the corresponding counter-
pieces to attach the column heating to the left-hand inside
wall of the Separation Center.
3 Connect column heating
• Insert 6.2108.120 Cable at connection 38 of the column heat-
ing (see Fig. 6) and at connection 23 on the rear panel of the
Separation Center (see Fig. 5).
18
819 IC Detector / 820 IC Separation Center
2.2 Installation of accessories
820
6.2108.120
Fig. 11: Connection 820 – column heating
2.2.3 Connection of syringe and aspirating tubing
For manual filling of the sample loops mounted on the injection valves,
the 6.2816.020 Syringe and the PTFE aspirating tubing already screwed
to the valve are needed. These accessories are mounted or adjusted as
follows:
1 Connect syringe
• Push 6.2816.020 Syringe (without needle) as far as it will go
into connection socket 13 (left for valve A, right for valve B)
(see Fig. 4).
2 Lead aspirating tubing to the outside
• Lead the PTFE aspirating tubing 63 (see Fig. 21, Fig. 22 resp.
Fig. 23) through one of the feedthroughs 14 at the front or
side of the separation center (see Fig. 4 and Fig. 5) to the
outside.
2.2.4 Connection of the drain tube
The 820 IC Separation Center has a connection at the rear to which a
drain tube for discharged liquids can be attached. Proceed as follows:
1 Connect drain tube
• Mount 6.1816.00 Silicone tubing on connection nipple 33
(see Fig. 5).
2 Lead drain tube to collecting vessel
• Lead the other end of the drain tube to a suitable collecting
vessel and fix in place.
2.2.5 Connection of the 6.5324.000 Bottle rack (option)
The optional available 6.5324.000 Bottle rack for supply vessels can be
placed on top of the IC system tower. The accessories include the supply vessels for eluent (2 L), regeneration solution (1 L) and rinsing solution (1 L). For the connection of the supply capillaries leading to the 818
IC Pump and the suppressor module, see the instructions given on the
enclosed leaflet.
819 IC Detector / 820 IC Separation Center
19
2 Installation
2.3 Electrical connection
2.3.1 Connection to 830 IC Interface
The instruments are controlled via the Metrohm «IC Net» software. They
are normally connected to a PC via an 830 IC Interface.
The 820 IC Separation Center can be operated in two different ways.
It can operated via the 819 IC Detector, or included as an independent
instrument in the «IC Net» via the 830 IC Interface. Both the cable
connections and the installation under «IC Net» depend on the type of
control.
Control of the Separation Center via 819
Normally the Separation Center is controlled with «IC Net» via the time
program of the detector. When a system is being set up either
to IC Detector
or 820.03X0 to IC Detector must be selected for the Separa-
tion Center in «IC Net».
In a 2-channel system each channel of the Separation Center is controlled via the time program of the assigned detector.
820.02X0
6.2115.070
6.2134.130
6.2134.090
830
819
820
PC
6.2134.100
6.2134.040
6.2125.090
PC
6.2134.100
6.2115.070
6.2134.130 6.2115.070
6.2134.090
830
819 B
819 A
820
6.2134.040
6.2134.130
6.2134.090
6.2125.090
6.2125.090
One-channel system Two-channel system
Fig. 12: Connection of 820 via 819 to 830
20
819 IC Detector / 820 IC Separation Center
2.3 Electrical connection
1 Connect 819 to 820
• Use 6.2125.090 Cable to connect Connection 10 “IC Separa-
tion Center” of the 819 IC Detector to Connection 30 “IC Detector” of the 820 IC Separation Center (see Fig. 12).
For the 2-channel versions 820.0X20 additionally:
• Use a further 6.2125.090 Cable to connect Connection 10 “IC
Separation Center” of the second 819 IC Detector to Connection 27 “IC Detector” of the 820 IC Separation Center
(see Fig. 12).
2 Connect 819 to 830
• Use 6.2134.090 Cable (830 accessory) to connect Connec-
tion 9 “RS 232” of the 819 IC Detector to a free RS 232 interface of System 1 of the 830 IC Interface (see Fig. 12).
• Use 6.2134.130 Cable to connect Connection 5 “Output” of
the 819 IC Detector to analog connection “Channels 1/2” of
System 1 of the 830 IC Interface (see Fig. 12).
For 2-channel versions 820.0X20 additionally:
• Use a further 6.2134.090 Cable (830 accessory) to connect
Connection 9 “RS 232” of the second 819 IC Detector to a
free RS 232 interface of System 2 of the 830 IC Interface
(see Fig. 12).
• Use 6.2134.130 Cable to connect Connection 5 “Output” of
the 819 IC Detector to analog connection “Channels 3/4” of
System 2 of the 830 IC Interface (see Fig. 12).
3 Connect 820 to 830
This cable connection starts data recording (chromatogram
window turns blue) when the valve in the 820 is switched to
INJECT via a program command; not necessary if valve is operated manually:
• Use 6.2115.070 Cable to connect the positions Integr. and
Start of terminal block 31 of the 820 IC Separation Center
to the event line Start of System 1 of the 830 IC Interface
(see Fig. 12).
For 2-channel versions 820.0X20 additionally:
• Use a further 6.2115.070 cable to connect the positions In-
tegr. and Start of terminal block 26 of the 820 IC Separation Centers to the event line Start of System 2 of the 830
IC Interface (see Fig. 12).
Take care that each of the RUN connections at 830 and 820 and the
COM connections at 830 and 820 are connected to each other.
4 Connect column heating to 830
For 820.02X0 only:
• Use 6.2134.040 Cable to connect RS 232 interface 22 of the
820 IC Separation Center to a free RS 232 interface of the
830 IC Interface (see Fig. 12).
819 IC Detector / 820 IC Separation Center
21
2 Installation
Separate control of the Separation Center
Alternatively you have the possibility of controlling the Separation Center with its own time program from «IC Net». This requires you to select
the appropriate
terface, 820.0310 to Interface, 820.0320 to Interface or 820.0330 to Interface for
820.0210 to Interface, 820.0220 to Interface, 820.0230 to In-
the Separation Center when setting up the systems in «IC Net».
6.2115.070
6.2128.100
6.2134.130
6.2134.090
830
819
820
PC
6.2134.100
6.2134.040
Fig. 13: Connection of 819 and 820 to 830
1 Connect 819 to 830
• Use 6.2134.090 Cable (830 accessory) to connect Connec-
tion 9 “RS 232” of the 819 IC Detector to a free RS 232 interface of System 1 of the 830 IC Interface (see Fig. 13).
• Use 6.2134.130 Cable to connect Connection 5 “Output” of
the 819 IC Detector to the analog connection “Channels
1/2” of System 1 of the 830 IC Interface (see Fig. 13).
22
819 IC Detector / 820 IC Separation Center
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