Metrohm 813 User Manual
CH-9101 Herisau/Switzerland
Phone +41 71 353 85 85
Fax +41 71 353 89 01
E-Mail info@metrohm.ch
http://www.metrohm.com
813 Compact Autosampler
Program version 5.813.0010
Instructions for use
8.813.1003 06.2001 / dm
D. Möckli
Teachware
Metrohm Ltd.
Oberdorfstr. 68
CH-9100 Herisau
st
1
Edition 2001
Copyright © 2001 by Metrohm Ltd. All rights reserved.
This manual was carefully produced. Nevertheless the author does not warrant the
information contained herein to be free of errors. The author appreciates your
comments and feedback.
© Metrohm Ltd. 2001
Printed in Switzerland
Table of content
1
2
Introduction ......................................................1
1.1 Instrument description .................................................................................1
1.2 Parts and controls.........................................................................................2
1.3 Rear view........................................................................................................4
1.4 The keyboard .................................................................................................5
Installation........................................................6
2.1 Setting up the instrument.............................................................................6
2.1.1 Packaging ..................................................................................................6
2.1.2 Delivery check ............................................................................................6
2.1.3 Location......................................................................................................6
2.2 Mains connection ..........................................................................................7
2.2.1 Setting the mains voltage...........................................................................7
2.2.2 Fuses..........................................................................................................8
2.2.3 Mains cable and mains connection...........................................................8
2.2.4 Switching the instrument on/off..................................................................9
2.3 Attaching the accessories............................................................................9
2.3.1 Connecting the keyboard...........................................................................9
2.3.2 Installing the plug cover .............................................................................9
2.3.3 Installing the needle .................................................................................10
2.3.4 Installing the splash protection ................................................................11
2.3.5 Placing the sample rack...........................................................................12
2.3.6 Adjusting the sample rack .......................................................................12
2.3.7 Tubing connections..................................................................................14
2.3.8 Tubing connection to Metrohm 761 / 790 IC–Systems ...........................15
2.3.9 Tubing connection to a Metrohm 747 VA Stand......................................16
2.3.10 Tubing connection to a Metrohm Computrace 757.................................16
2.4 Instrument connections..............................................................................17
2.4.1 Remote interface ......................................................................................17
2.4.2 Interconnection with Metrohm IC systems...............................................17
2.4.3 Interconnection with a Metrohm 746 VA Trace Analyzer .........................18
2.4.4 Interconnection with a Metrohm 757 VA Computrace .............................19
2.4.5 Interconnection with other instruments ....................................................19
3
4
Operation ........................................................20
3.1 Keyboard functions.................................................................................... 20
3.2 Configuration .............................................................................................. 21
3.2.1 Course of configuration ...........................................................................23
3.3 RAM initialization........................................................................................ 24
3.4 The methods ............................................................................................... 25
3.5 The Metrohm IC control software ............................................................. 27
3.5.1 Operational conditions............................................................................. 27
3.6 Modular Metrohm IC System 732/733 ....................................................... 28
3.7 Metrohm 746 VA Trace Analyzer ............................................................... 28
3.7.1 Monitoring ................................................................................................28
3.7.2 Command sequence ............................................................................... 28
3.7.3 Conditions to be met ...............................................................................29
Appendix..........................................................30
4.1 Error Messages........................................................................................... 30
4.2 Sequences................................................................................................... 31
4.3 Technical specifications ............................................................................ 35
4.4 Maintenance and servicing........................................................................ 37
4.4.1 Maintenance by Metrohm service ...........................................................37
4.4.2 Care of the unit.........................................................................................37
4.4.3 Replacing the pump tubing .....................................................................37
4.5 Warranty and conformity ........................................................................... 39
4.5.1 Warranty ................................................................................................... 39
4.5.2 Certificate of Conformity and System Validation .....................................40
4.6 Standard equipment................................................................................... 42
4.7 Optional accessories.................................................................................. 46
5
Index................................................................48
Table of Figures
Fig. 1 Side view ........................................................................................................ 2
Fig. 2 Rear view........................................................................................................ 4
Fig. 3 Keyboard ........................................................................................................ 5
Fig. 4 Setting the mains voltage .............................................................................. 8
Fig. 5 Keyboard connection..................................................................................... 9
Fig. 7 Installing the splash protection.................................................................... 11
Fig. 8 Sample rack placing .................................................................................... 12
Fig. 9 Installing the pump tubing ........................................................................... 15
Fig. 10 Connection of a 747 VA Stand ..................................................................... 16
Fig. 11 Measuring head of the747 VA Stand ........................................................... 16
Fig. 12 Interconnection with 761 or 790 IC System ................................................ 17
Fig. 13 Interconnection with a modular Metrohm IC System .................................. 18
Fig. 14 Interconnection with a Metrohm 746 VA Trace Analyzer............................. 18
Fig. 15 Interconnection with 757 VA Computrace................................................... 19
Fig. 16 Configuration................................................................................................ 23
Fig. 17 Time program of the IC control software ..................................................... 27
1 Introduction
1 Introduction
1.1 Instrument description
The 813 Compact Autosampler can be used for automating different tasks, e.g. ion chromatographic determinations with
the Metrohm IC system instruments or voltammetric determinations with the Metrohm 746 Trace Analyzer or 757 VA Computrace . The sample rack provided may hold up to 36 vials of 2.5
or 11 mL volume respectively. Sample tubes made of polypropylene are standard. To protect the samples from external contamination, the tubes can be hermetically sealed.
Sample introduction from the 813 Compact Autosampler is
achieved by means of its integrated peristaltic pump. The sample
is conveyed by the pump through the capillary into e. g. the sample loop of the injector located within an ion chromatograph. A
PEEK needle for sealed or a PEEK tube for open sample tubes
can be used alternatively.
Preprogrammed methods for the most common modes of operation allow to use the 813 Compact Autosampler directly, without
programming effort.
813 Compact Autosampler
1
1.2 Parts and controls
0
3
4
5
7
8
9
012
6
1.2 Parts and controls
56 7
4
3
2
8
25
1
9
1
11
26
1
1
1
1
1
1
Metrohm
DES FU SIB L ES DE 2 50V
100-120V
USE ONLY WITH 250V
UNIQUEMENT AVEC
FUSES/EMPLOYER
220-240V
2
24
23
1
22
21
2
Fig. 1 Side view
Safety note:
Never operate the instrument without splash protection and plug
cover being mounted.
The plug cover prevents any contamination of the connectors,
caused by spilled solvents or chemicals.
813 Compact Autosampler
1 Introduction
1
Splash protection 6.2751.040
Must be installed always in operation!
14
Holding clamp
For locking the tubing cartridge into
place
2
Needle
PEEK needle 6.1835.020 or PEEK
tube 6.1835.010 or 6.1835.030
3
Lift
4
Steel holder 4.766.4330
attached (part of 6.2833.000 Needle
holder)
5
PEEK compression fitting
4.766.4320
for connection of PEEK capillary
(part of 6.2833.000 Needle holder)
6
PEEK compression fitting
6.2744.010
7
PEEK capillary 6.1831.050
(40 cm)
connection needle – pump tubing
7
15
Snap-action lever
For releasing the tubing cartridge
16
Screw thread for splash
protection
17
PEEK capillary 6.1831.060 or
6.1822.410 (1 m or 1.2 m)
For conveying the sample to e.g. an
injection valve
18
Tower
19
Remote connection
20
Plug cover 6.2752.010
8
Pump tubing 6.1826.040
For conveying the sample
9
Screw thread for splash
protection
10
Mounting pin
For attaching the tubing
cartridge
11
Tubing cartridge 6.2755.000
For 6.1826.0X0 pump tubing
6.1826.0X0
12
Pump drive
Roller head with contact rollers
13
Contact pressure lever
For adjusting the contact pressure
21
Fuse holder
Changing the fuses, see section 2.2.2
22
Mains connection plug
Mains connection, see section 2.2.3
23
Mains switch
For switching the instrument on/off:
I = ON 0 = OFF
24
Sample rack 6.2041.7500
25
PP sample tube 6.2743.050
(can be sealed with 6.2743.060 PE
caps)
26
Needle guide
813 Compact Autosampler
3
1.3 Rear view
1.3 Rear view
9
11
9
18
16
24
20
9
Mounting screws for
splash protection
Type 1.813.
Made by Metrohm Herisau Switzerl and
Fig. 2 Rear view
24
Sample rack 6.2041.750
Keyboard
16
25
27
28
4
11
Tubing cartridge
6.2755.000
For 6.1826.0X0 pump tubing
16
Mounting screws for
splash protection
18
Tower
20
Plug cover 6.2752.010
25
PP sample tubes 6.2743.050
(can be sealed with 6.2743.060 PE
caps)
27
Serial number
28
Keyboard connection
813 Compact Autosampler
1 Introduction
1.4 The keyboard
813 Compact Aut osampl er
• The <START> key starts a sample series or
continues an interrupted series.
• The <STOP> key terminates a sample series
or resets (if pressed twice) the 813 Compact
Autosampler (RESET).
• The <HOLD> key interrupts the course of a
sample series and establishes the 'HOLD'
state. The green LED blinks. With the
<START> key the interrupted sample series
can be continued.
HOLD
RESE T
STOP
START
Met r ohm
6.2142. 110
Fig. 3 Keyboard
• The green LED indicates the status of the
Autosampler.
The LED is lit in the 'ready' state.
-
It blinks steady during the course of a
-
method.
It blinks in fast frequency when a series is
-
interrupted ('HOLD' state).
• The red LED indicates error states or parameter settings.
If an error occured the type of error is indi-
-
cated by the number of blinks, see Ap-
pendix, Error messages.
813 Compact Autosampler
5
2.1 Setting up the instrument
2 Installation
2.1 Setting up the instrument
2.1.1 Packaging
The 813 Compact Autosampler is supplied together with the
separately packed accessories in special packagings containing
shock-absorbing foam linings designed to provide excellent protection. The actual instrument is packed in an evacuated polyethylene bag to prevent the ingress of dust. Please store all these
special packagings as only they can assure damage-free transport of the instrument.
2.1.2 Delivery check
After receipt, immediately check whether the shipment is complete and undamaged (compare with delivery note and list of accessories in section 4.6). In the case of transport damage, see instructions in section 4.5.1 "Warranty".
2.1.3 Location
Position the instrument in the laboratory at a location convenient
for operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals.
Do not operate the 813 Compact Autosampler without splash
protection
Take precautions to ensure that any leaks from pump tubings or
connections cannot cause more damage.
Take precautions to ensure that any leaks from pump tubings or
connections cannot cause more damage.
1
and plug cover 20 being installed!
6
813 Compact Autosampler
2 Installation
2.2 Mains connection
Follow the instructions below for connecting to the power
supply. If the instrument is operated with the mains voltage set
wrongly and/or wrong mains fuse there is a danger of fire!
2.2.1 Setting the mains voltage
Before switching on the 813 Compact Autosampler for the first
time, check that the mains voltage set on the instrument (see Fig.
4) matches the local mains voltage. If not
on the instrument as follows:
Disconnect mains cable
1
Disconnect mains cable from mains connection plug 22 of the
813 Compact Autosampler.
, reset the mains voltage
Remove fuse holder
2
Using a screwdriver, loosen fuse holder 21 beside the mains
connection
Check fuse
3
Carefully take the fuse installed for the desired mains voltage out
of fuse holder
fuse in the fuse holder is marked by the white arrow imprinted
next to the mains voltage range):
100…120 V 0.5 A (slow-blow)
Metrohm-Nr. U.600.0013
220…240 V 0.25 A (slow-blow)
Metrohm-Nr. U.600.0010
Insert fuse
4
Change fuse if necessary and reinsert in fuse holder 21.
Install fuse holder
5
Depending on the desired mains voltage, insert fuse holder 21
in the 813 Compact Autosampler so that the corresponding
mains voltage range can be read normally and the adjacent
white arrow points to the white bar imprinted on the right side of
the fuse holder (see Fig. 4).
22
and take out completely.
21
and check its specifications (the position of the
813 Compact Autosampler
7
2.2 Mains connection
23
22
21
2.2.2 Fuses
100 – 120 V
220 - 240 V
One of the two fuses 0.5 A/slow-blow for 100…120 V or 0.25
A/slow-blow for 220…240 V is installed in fuse holder
813 Compact Autosampler as standard
Ensure that the instrument is never put into operation with fuses
of another type, otherwise there is danger of fire!
220 – 240 V
100
-
120 V
100 - 120 V
220 - 240 V
Fig. 4 Setting the mains voltage
21 Fuse holder
22
Mains connection plug
23
Mains switch
21
of the
For checking or changing fuses, proceed as described in section
2.2.2.
2.2.3 Mains cable and mains connection
Mains cable
The instrument is supplied with one of three mains cables
• 6.2122.020 with plug SEV 12 (Switzerland, …)
• 6.2122.040 with plug CEE(7), VII (Germany, …)
• 6.2133.070 with plug NEMA 5-15 (USA, …)
which are three-cored and fitted with a plug with an earthing pin.
If a different plug has to be fitted, the yellow/green lead (IEC
standard) must be connected to protective earth (protection class
1).
Any break in the earthing inside or outside the instrument can
make it a hazard!
Mains connection
Plug the mains cable into mains connection plug 22 of the 813
Compact Autosampler (see Fig. 4).
8
813 Compact Autosampler
2 Installation
2.2.4 Switching the instrument on/off
The 813 Compact Autosampler is switched on and off using the
mains switch
23
.
2.3 Attaching the accessories
For attaching the accessories at the 813 Compact Autosampler,
proceed in the order described below.
2.3.1 Connecting the keyboard
Connecting the keyboard
1
Connect the 6.2142.110 keyboard
to the keyboard connection
"Keyboard". For disconnection,
press the plug together slightly on
both sides.
Switch on the instrument
2
Switch on the 813 Compact
Autosampler with mains switch
The instrument is initialized and
the lift is raised completely. The
green LED lights up.
2.3.2 Installing the plug cover
28
Type 1.760.001 0 Nr.
0010/01104
Keyboard
Made by Metrohm Herisau Switzerland
23
.
Fig. 5 Keyboard connection
813 Compact Autosampler
To prevent any contamination of the mains and remote connection by spilled solvents or chemicals, the 6.2752.010 plug cover
must always be installed when operating the 813 Compact
Autosampler!
Install the plug cover
above mains connection plug
20
in the corresponding guide groove
22
and remote connection 19 (see
Fig. 1 and Fig. 2).
9
2.3 Attaching the accessories
2.3.3 Installing the needle
Remove PEEK compression fitting 5
1
Remove PEEK compression fitting 5 screwed onto the needle
holder
Insert needle
2
Fix needle
3
4
.
• Needle
6.1835.010 PEEK tube) is inserted a short distance into the
opening of steel holder
• Push PEEK round seal
smaller end of the seal must face upwards.
• Screw PEEK compression fitting
pressing needle
• Tighten compression fitting
(never
2
(6.1835.010 PEEK needle or 6.1835.020 or
4
.
29
over needle 2 from above. The
5
into steel holder 4 while
2
gently upwards from below.
5
in steel holder 4 by hand
use tools!).
2 PEEK needle (6.1835.010) or PEEK
tube (6.1835.020 or 6.1835.030)
4 Steel holder (4.766.4330)
(part of 6.2833.000 Needle holder)
5 PEEK compression fitting
(4.766.4320)
(part of 6.2833.000 Needle holder)
6 PEEK compression fitting
(6.2744.010)
7 PEEK capillary (6.1831.050)
29 PEEK ferrule
If you are using the 6.1835.020 or 6.1835.030 PEEK tube as needle, the
sample tubes must not be sealed with caps because they cannot be
pierced by the PEEK tube and this could damage the tube!
7
6
5
4
2
Fig. 6 Needle installation
5
29
2
10
813 Compact Autosampler
2 Installation
2.3.4 Installing the splash protection
To avoid any danger of injury by the needle, the 6.2751.070 splash
protection must always be installed when operating the 813 Compact
Autosampler!
Remove holding screws
1
Remove the holding screws 16 and the washer mounted on the
screw threads
Remove protective film from splash protection
2
Remove the plastic film glued on both sides of the splash
protection
Install splash protection
3
Attach splash protection 1 onto the screw threads 9 and 16 at
the tower
using the 6.2621.100 Allen key.
9
at tower 18 using the 6.2621.100 Allen key.
1
.
18
and fix it with the holding screws 9 and the washer
1 Splash protection
6.2751.040
9 Holding screw
16 Holding screw
9
1
16
Fig. 7 Installing the splash protection
813 Compact Autosampler
11
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