Metrohm 871 User Manual
871 Advanced Bioscan
CH-9101 Herisau/Switzerland
E-Mail info@metrohm.com
Internet www.metrohm.com
Instructions for Use
8.871.1003
CH-9101 Herisau/Switzerland
E-Mail info@metrohm.com
Internet www.metrohm.com
871 Advanced Bioscan
8.871.1003 Instructions for Use
8.871.1003 01.2007 / chs
Teachware
Metrohm AG
Oberdorfstrasse 68
CH-9101 Herisau
teachware@metrohm.com
1. Edition 2007
These instructions are protected by copyright. All rights reserved.
Although all the information given in these instructions has been checked with great care, errors
cannot be entirely excluded. Should you notice any mistakes please inform the author at the
address given above.
1.1 Instrument description
Table of contents
1 Introduction ................................................................ 1
1.1 Instrument description ................................................................................1
1.2 Parts and controls .......................................................................................2
1.2.1 Front.................................................................................................................... 2
1.2.2 Rear .................................................................................................................... 3
1.2.3 Interior................................................................................................................. 4
1.3 Information on the Instructions for Use .....................................................6
1.3.1 Organization .......................................................................................................6
1.3.2 Notation and pictograms.................................................................................... 7
1.4 Safety notes .................................................................................................8
1.4.1 Electrical safety................................................................................................... 8
1.4.2 General precautionary rules ............................................................................... 8
2 Installation ................................................................. 9
2.1 Setting up the instrument............................................................................9
2.1.1 Packaging...........................................................................................................9
2.1.2 Check.................................................................................................................. 9
2.1.3 Location .............................................................................................................. 9
2.1.4 Arrangement of the instruments......................................................................... 9
2.2 Mains connection ........................................................................................9
2.2.1 Fuses ................................................................................................................ 10
2.2.2 Mains cable and mains connection ................................................................. 10
2.2.3 On/off switching of the instruments ................................................................. 11
2.3 Connection to PC ......................................................................................11
2.3.1 Connecting cable .............................................................................................11
2.3.2 Software installation.......................................................................................... 11
2.3.3 First Login ......................................................................................................... 12
2.3.4 Create a system ............................................................................................... 12
2.4 Capillary connections ...............................................................................12
2.4.1 Connection of the 6.5324.000 Bottle rack (option).......................................... 12
2.4.2 Connect Drainage tube .................................................................................... 13
2.4.3 Connecting the pulsation absorber.................................................................. 13
2.4.4 Connection of the sample path........................................................................ 13
2.4.5 Installation of the precolumn ............................................................................ 14
2.4.6 Installation of the IC column............................................................................. 14
2.4.7 Measuring cell .................................................................................................. 15
3 Basic principles........................................................ 16
3.1 Introduction ...............................................................................................16
3.2 Measuring conditions................................................................................16
3.3 Pulsed amperometric detection ...............................................................17
3.3.1 Optimization of the PAD parameters ............................................................... 18
3.4 Optimization of the measuring potential ..................................................19
4 Notes - Maintenance - Faults................................... 22
4.1 Handling the 871 Advanced Bioscan .......................................................22
4.2 Practical notes on ion chromatography...................................................22
4.2.1 Separating columns ......................................................................................... 22
4.2.2 High-pressure pump ........................................................................................ 23
4.2.3 Eluents .............................................................................................................. 24
871 Advanced Bioscan / Instructions for Use 8.871.1003
I
1 Introduction
4.2.4 Connections ..................................................................................................... 24
4.3 Maintenance and servicing .......................................................................25
4.3.1 General information.......................................................................................... 25
4.3.2 Shutdown ......................................................................................................... 25
4.3.3 Cleaning the working electrode ....................................................................... 26
4.3.4 Changing separating columns......................................................................... 26
4.4 Faults and malfunctions............................................................................28
4.4.1 Malfunctions and their rectification .................................................................. 28
4.5 Instrument test with the dummy cell ........................................................30
4.6 Diagnostic tests / Validation / GLP ...........................................................31
5 Appendix ................................................................... 32
5.1 Technical data............................................................................................32
5.1.1 Measuring unit.................................................................................................. 32
5.1.2 Operating modes ............................................................................................. 32
5.1.3 Autozero ........................................................................................................... 33
5.1.4 Injection valve................................................................................................... 33
5.1.5 Oven ................................................................................................................. 33
5.1.6 Mains connection............................................................................................. 33
5.1.7 RS 232 Interface............................................................................................... 33
5.1.8 I/O lines ............................................................................................................ 33
5.1.9 Signal output .................................................................................................... 34
5.1.10 Safety specifications ........................................................................................ 34
5.1.11 Electromagnetic compatibility (EMC) .............................................................. 34
5.1.12 Ambient temperature ....................................................................................... 34
5.1.13 Housing ............................................................................................................ 34
5.2 Standard equipment ..................................................................................36
5.3 Optional accessories.................................................................................39
5.4 Warranty and Conformity ..........................................................................44
5.4.1 Warranty ........................................................................................................... 44
5.4.2 Declaration of Conformity ................................................................................ 45
5.4.3 Quality Management Principles ....................................................................... 46
5.5 Index...........................................................................................................47
II
871 Advanced Bioscan / Instructions for Use 8.871.1003
1.1 Instrument description
List of figures
Figure 1: Front 871 Advanced Bioscan ....................................................................2
Figure 2: Rear 871 Advanced Bioscan ..................................................................... 3
Figure 3: Interior 871 Advanced Bioscan.................................................................. 4
Figure 4: Mains connection plug ............................................................................ 10
Figure 5: Potentials applied during pulsed amperometric detection (PAD) ........... 17
Figure 6: Example of a hydrodynamic voltammogram of a substance (A) with the
additional presentation of the measured values for the pure eluent (B) . 19
Figure 7: Example of a scan of a substance (A) with the additional
representation of the scan of the pure eluent (B) .................................... 20
871 Advanced Bioscan / Instructions for Use 8.871.1003
III
1.1 Instrument description
1 Introduction
1.1 Instrument description
The 2.871.0010 Bioscan is a PC-controlled measuring instrument for
the sensitive analysis of carbohydrates by ion chromatography using
pulsed amperometric detection. The compact housing of the 871 Advanced Bioscan contains several IC system components:
• Column compartment – the perfect insulation of the housing cre-
ates not only stable thermal conditions for the separating columns,
but also shields the system from electromagnetic interference; in
addition to the column it also contains the detection cell, pulsation
absorber and injection valve.
• Oven – amperometric determinations require extremely stable
thermal conditions. The built-in column compartment oven ensures
that all important components can be set exactly to a temperature
from 10°C above room temperature to 45 °C with a stability of
0.1°C.
• All components that come into contact with the eluent and the
sample, except the electrodes, are metal-free.
• Signal converter – the 871 Advanced Bioscan contains its own
analog/ digital converter for the detector signal. This means that
complete control of the instrument is possible from a PC via an
RS232 interface.
• Injection valve – for individual injections or for use with a sample
changer
Together with the 818 IC Pump, the 871 Advanced Bioscan forms a
complete IC system for carbohydrate analysis. Operation is via a PC
connected to the RS232 interface and uses the included «IC Net» control and evaluation program. This software fulfills all the requirements
which are placed today on a modern integration software: 1-point or
multi-point calibration, internal or external standard, selectable algorithms for non-linear calibration, numerous Integration modes with selectable parameters and integration events, various peak recognition
methods, peak editor, free scaling, superimposition of several chromatograms, post-treatment of chromatograms, high-performance GLPconform report generator with output interfaces for monitor, printer and
external databases.
871 Advanced Bioscan / Instructions for Use 8.871.1003
1
1 Introduction
1.2 Parts and controls
In this Section you will find the numbers and designations of the parts
and controls of the 871 Advanced Bioscan. The numbering applies
throughout the Instructions for Use, i.e. bold numbers in the text (e.g.
4
) refer to the parts and controls illustrated here.
1.2.1 Front
1
2
4
3
Figure 1: Front 871 Advanced Bioscan
Door to interior
1
Feed through for capillary
2
Inlet for eluent and sample
2
871 Advanced Bioscan / Instructions for Use 8.871.1003
Drain opening for leaking liquid
3
Feed through for capillary
4
outlet for eluent and sample
1.2 Parts and controls
1.2.2 Rear
5
6
7
8
Figure 2: Rear 871 Advanced Bioscan
Remote connection A
5
Mains switch
6
to switch instrument on and off:
I = ON 0 = OFF
Fuse holder
7
changing the fuses, see section 2.2.1
12
9
10
11
Connection C Manual valve
9
(not used with IC Net)
Remote connection B
10
RS232 interface
11
Connection of the PC; see chapter 5.1
for details
Mains connection plug
8
mains connection, see section 2.2
871 Advanced Bioscan / Instructions for Use 8.871.1003
Analog signal
12
AD/DA-converted signal (not used
with IC Net; see chapter 5.1)
3
1 Introduction
1.2.3 Interior
13
14
15
16
17
18
19
20
34
33
32
31
30
Metrosep
Carb 1
21
22
23
24
25
26
27
Figure 3: Interior can
871 Advanced Bios
28
29
Column connection capillary
13
Connection to Flow cell
Flow cell
14
see section 2.4.7
PEEK capillary
4
871 Advanced Bioscan / Instructions for Use 8.871.1003
1.2 Parts and controls
Electrode cable
15
(black)
connection for reference electrode
IC column
16
e.g. Metrosep Carb 1 (6.1013.000)
for carbohydrate analysis
Column holder
17
Injection valve
18
see section 2.4.4
Sample loop
19
Connection capillary
20
connection injection valve 18 – precolumn 22
PEEK capillary
Connection for electrode cable
21
Precolumn
22
see section 2.4.5
Connection capillary
23
connection precolumn 22 – IC column 16
PEEK capillary
Connection capillary
24
for sample. Connection to injection
valve 18
PEEK capillary
Connection capillary
25
for eluent. connection 818 IC Pump –
Pulsation absorber 26
PEEK capillary
Pulsation absorber
26
6.2620.150
Connection capillary
27
connection Pulsation absorber 26 –
injection valve 18
PEEK capillary
Oven
28
Connection capillary
29
for sample. Connection injection
valve 18 – Waste
PEEK capillary
Oven heating fan
30
Connection capillary
31
Connection Flow cell 14 – Waste
PEEK capillary
Electrode cable (blue)
32
connection for auxiliary electrode
Electrode cable (red)
33
connection for working electrode
Measuring cell holder
34
871 Advanced Bioscan / Instructions for Use 8.871.1003
5
1 Introduction
1.3 Information on the Instructions for Use
Please r roug e In ction r Use carefully before you put
the 871 Advanced Bioscan into operation. The Instructions for Use
contain information and warnings to which the user must pay attention
in order to assure safe operation of the instrument.
ead th h thes stru s fo
1.3.1 Organization
These Instructions for Use 8.871.1003 for the 871 Advanced Bioscan
provide a comprehensive overview of installation, startup procedure,
operation, fault rectification and technical specifications of this instrument. The Instructions for Use are organized as follows:
Sect. 1 Introduction
Sect. 2 Installation
Sect. 3 Basic principles
Sect. 4 Notes - Maintenance - Faults
Sect. 5 Appendix
General description of instrument, parts and controls and
safety notes
Installation of instrument and accessories
Information about the pulsed amperometric detection
Notes on ion chromatography, maintenance, fault rectification, diagnostic tests, validation
Technical data, standard equipment, options, warranty,
declarations of conformity, index
To find the required information on the instruments, use either the Ta-
ble of contents or the Index at the back.
6
871 Advanced Bioscan / Instructions for Use 8.871.1003
1.3 Information on the Instructions for Use
1.3.2 tograms
Notation and pic
The following notations and pictograms (symbols) are used in these Instructions for Use:
Fill entry
SYSTEM STATE P
<OK>
23 Part or control of 871
Menu item, parameter or
v
alue
in the software
rogram window
the software
in
Button
in the software
azard
H
This symbol draws attention to a
possible danger to life or of injury if
t e associated directio
h ns are not
followed correctly.
arning
W
This symbol draws attention to possible damage to instruments or instrument parts if the associa
ctions are not followed correctly.
re
ted di-
Caution
his symbol marks important infor-
T
mation. First read the associated dir ctions before you continu
e e.
Comment
This symbol marks additional information and tips.
871 Advanced Bioscan / Instructions for Use 8.871.1003
7
1 Introduction
1.4 Safety notes
1.4.1 Electrica
• Mains co
• Op ced Bioscan ening the 871 Advan
• Protection against static charges
l safety
W
hile electrical safety in the handling of the 871 Advanced Bioscan is
a
ssured in the context of the specifications EN 61010-1 (protection
c
lass
I, degree of protection IP20), the following
noted:
nnection
Setting of the mains voltage, checking the mains fuse and the
mains connection must be effected in accordance with the instruc-
tions in Section 2.2.
If the 871 A
instrument m pened nor must parts be removed from it,
o h er of coming into contact with components
therwise t ere is a dang
which are l ment from all
vo n it and ensure that the mains cable
ltage sources before you ope
is disconne
dvanced Bioscan is connected to the power supply, the
ust not be o
ive. Therefore always disconnect the instru
cted from mains connection
8
points should be
!
E components are sensitive to static charging and can be
lectronic
destroyed b
inside the 871 Advanced Bioscan, you should ground yourself and
any tools you are using by touching a grounded object (e.g. housing
of the instrument or a radiator) to eliminate any static charges which
exist.
y discharges. Before you touch any of the components
1.4.2 General precautionary rules
• Handling of solvents
Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.
• Never block drain opening for spilled liquids
On the bottom of the interior directly below the front door there is a
3
drain opening
3
. Due to safety consideration, take care that these openings may
never be blocked.
. Spilled liquids can flow directly to the drain opening
8
871 Advanced Bioscan / Instructions for Use 8.871.1003
2.1 Setting up the instrument
2 Installation
2.1 Setting up the instrumen
2.1.1 Packaging
The 8 an is ith the separately
71 Advanced Biosc supplied together w
packed accessories in special p ining shock-absorbing
foam linings designed to provide excellent protection. The actual
instruments are packed in an evacuated polyethylene bag to prevent
the ingress o lease store ckaging as only it can
assure transport of the instruments free from damage.
2.1.2 Check
To unpack the 871 Advanced Bioscan, lift it from its box by both
hands. Never lift the 871 Advanc n at its front doors, but at its
sides.
After receipt, immediately check nt is complete and
has arrived without damage (compare with delivery note and list of
accessories in section 5.2). In the case of transport damage, see
instructions in section 5.4 "Warran
2.1.3 Location
f dust. P all this special pa
t
ackaging conta
ed Biosca
whether the shipme
ty".
Position the instruments in the l for
operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals. The same applies to all
other components of the IC syste
To avoid disturbing temperature
compartment, the entire system servoir
must be protected against direct sunlight.
2.1.4 Arrangement of the instruments
In general, the IC pumps should be set up at the very bottom and the
IC detectors at the very top.
2.2 Mains connection
Follow the instructions below for connecting to the power supply. If the
instrument is operated with wrong mains fuse, there is a danger of fire!
aboratory at a location convenient
m.
influences on the insulated column
including pump and eluent re
871 Advanced Bioscan / Instructions for Use 8.871.1003
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