Metrohm 792IC User Manual
CH-9101 Herisau/Switzerland
Phone ++41 71 353 85 85
Fax ++41 71 353 89 01
Internet www.metrohm.com
E-Mail info@metrohm.com
792 Basic IC
Program «792 PC Software 1.0»
Basic IC792
Basic
Basic
BasicBasic
POWER
8.792.1003 Instructions for Use
11.04.2001 / dö
Table of contents
Table of contents
1 Introduction
1.1 Instrument description.......................................................................................... 1
1.2 Parts and controls.................................................................................................. 3
1.3 Information on the Instructions for Use............................................................. 8
1.3.1 Organization ........................................................................................... 8
1.3.2 Notation and pictograms........................................................................ 9
1.4 Safety notes ..........................................................................................................10
1.4.1 Electrical safety..................................................................................... 10
1.4.2 General precautionary rules ................................................................. 10
2 Installation
2.1 Overview ................................................................................................................ 11
2.1.1 Flow chart ............................................................................................. 11
2.1.2 Connections in the 792 Basic IC .......................................................... 11
2.2 Setting up the instrument ................................................................................... 13
2.2.1 Packaging............................................................................................. 13
2.2.2 Check.................................................................................................... 13
2.2.3 Location ................................................................................................ 13
2.3 Attaching the accessories .................................................................................. 13
2.3.1 Connection of detector block............................................................... 13
2.3.2 Connection of syringe and aspirating tubing....................................... 14
2.3.3 Connection of the 6.5324.000 Bottle rack (option) .............................. 14
2.3.4 Connection of PEEK capillaries............................................................ 15
2.3.5 Filter unit PEEK ..................................................................................... 16
............................................................................................................
..........................................................................................................
1
11
2.4 Mains connection ................................................................................................. 17
2.4.1 Setting the mains voltage..................................................................... 17
2.4.2 Fuses .................................................................................................... 18
2.4.3 Mains cable and mains connection ..................................................... 18
2.4.4 On/off switching of the instrument ....................................................... 18
2.5 Connection to the PC ..........................................................................................19
2.5.1 Connecting cable ................................................................................. 19
2.5.2 Software installation.............................................................................. 19
2.5.3 Basic settings ....................................................................................... 20
2.6 High-pressure pump............................................................................................ 23
2.6.1 Removing the transport security screws.............................................. 23
2.6.2 Installing the pulsation dampener (option) .......................................... 23
2.6.3 Connecting the eluent bottle ................................................................ 25
2.6.4 Deaerating the pump and rinsing the pulsation dampener................. 25
2.7 Precolumns and separating columns............................................................... 28
2.7.1 General information on precolumns..................................................... 28
2.7.2 Precolumns with twin cartridge holder ................................................. 28
2.7.3 Precolumn glass cartridges with cartridge holder ............................... 30
2.7.4 IC anion precolumn SUPERSEP ..........................................................31
2.7.5 General information on separating columns........................................ 32
2.7.6 Selection of the sample loop................................................................ 32
2.7.7 Installation of the separating column without suppressor................... 32
2.7.8 Installation of the separating column with suppressor ........................ 34
792 Basic IC
I
Table of contents
2.8 Suppressor module ............................................................................................. 35
2.8.1 General information on suppressor module........................................ 35
2.8.2 Preparation of the peristaltic pump...................................................... 35
2.8.3 Connection of supply bottles ............................................................... 38
2.8.4 Connection of the suppressor module ................................................ 39
2.9 Putting into operation ......................................................................................... 41
2.9.1 Putting into operation without suppressor........................................... 41
2.9.2 Putting into operation with suppressor ................................................ 42
3 Operating tutorial
3.1 Requirements ....................................................................................................... 45
3.2 Preparations.......................................................................................................... 46
3.3 Calibration ............................................................................................................. 47
3.4 Sample determination ......................................................................................... 54
4 Operation
4.1 Fundamentals of the operation ......................................................................... 57
...............................................................................................................
4.1.1 Starting/closing the program ............................................................... 57
4.1.2 Glossary................................................................................................ 58
4.1.3 Overview of program windows............................................................. 59
4.1.4 Main window elements......................................................................... 60
4.1.5 Icons of the main window..................................................................... 60
4.1.6 Overview of file types............................................................................ 61
4.1.7 Context sensitive menus ...................................................................... 62
4.1.8 Keyboard and mouse functions........................................................... 62
4.1.9 Help ...................................................................................................... 63
.......................................................................................
45
57
4.2 Instrument and software settings ..................................................................... 64
4.2.1 Fonts..................................................................................................... 64
4.2.2 Security system .................................................................................... 64
4.2.3 Global settings ..................................................................................... 65
4.2.4 COM port.............................................................................................. 67
4.3 Systems ................................................................................................................. 68
4.3.1 System window .................................................................................... 68
4.3.2 System file handling ............................................................................. 68
4.3.3 System functions .................................................................................. 69
Start/stop hardware and record baseline .................................... 69
Start/stop determinations............................................................. 69
Print system parameters .............................................................. 70
4.3.4 PC icon ................................................................................................. 71
4.3.5 Watch window ...................................................................................... 71
4.3.6 Instrument icon..................................................................................... 72
Menu options for instrument icon ................................................ 72
System parameters for disconnected system ............................. 72
Instrument control for connected system .................................... 73
Time program ............................................................................... 74
Configuration ................................................................................ 76
Hardware settings ........................................................................ 76
4.3.7 System state window............................................................................ 78
Status messages.......................................................................... 78
Error messages ............................................................................ 79
II
792 Basic IC
Table of contents
4.4 Methods .................................................................................................................80
4.4.1 Method file handling ............................................................................. 80
4.4.2 Passport................................................................................................ 80
General ......................................................................................... 81
Sample.......................................................................................... 82
Column ......................................................................................... 83
Eluent ............................................................................................ 84
Comment ...................................................................................... 84
Method Log .................................................................................. 85
Data Log ....................................................................................... 86
4.4.3 Method setup........................................................................................ 87
General ......................................................................................... 87
Processing.................................................................................... 87
Math .............................................................................................. 88
4.4.4 Integration............................................................................................. 90
Setup............................................................................................. 91
Events ........................................................................................... 92
4.4.5 Calibration and quantification............................................................... 94
General information ...................................................................... 94
Notations....................................................................................... 95
External standard calibration........................................................ 96
Component table.......................................................................... 96
Peak identification ........................................................................ 98
Concentration table .................................................................... 100
Calibration curve......................................................................... 102
Update calibration ......................................................................104
Load and save calibration data.................................................. 105
Import and export calibration data ............................................. 105
Put out calibration curves ........................................................... 105
4.4.6 Report output...................................................................................... 106
Report options ............................................................................ 106
4.5 Chromatograms.................................................................................................. 118
4.5.1 Chromatogram window ...................................................................... 118
4.5.2 Chromatogram file handling............................................................... 119
Open chromatogram.................................................................. 119
Save chromatogram ................................................................... 120
Close chromatogram.................................................................. 120
Delete chromatogram................................................................. 120
Export chromatogram................................................................. 121
4.5.3 Graphical representation.................................................................... 122
Appearance ................................................................................ 122
Other graphical functions ........................................................... 126
4.5.4 Peak editor.......................................................................................... 127
Switching on/off the peak editor................................................. 127
Peak editor functions.................................................................. 127
Moving the cursor....................................................................... 128
4.5.5 Printing................................................................................................ 129
Page layout for printing .............................................................. 129
Printer settings ............................................................................ 130
Print preview ............................................................................... 130
Printing........................................................................................ 130
4.5.6 Miscellaneous functions ..................................................................... 130
Reintegration............................................................................... 130
Recalibration............................................................................... 130
Subtraction of a chromatogram ................................................. 131
Data compression ......................................................................131
Invert chromatogram .................................................................. 131
792 Basic IC
III
Table of contents
4.6 Batch reprocessing ...........................................................................................132
4.6.1 Batch reprocessing queue file handling ............................................ 132
Open batch reprocessing queue ............................................... 132
Create new batch reprocessing queue...................................... 132
Save batch reprocessing queue ................................................ 132
4.6.2 Perform batch reprocessing............................................................... 133
Reprocess options window........................................................ 133
Merge chromatograms .............................................................. 136
4.6.3 Batch reprocessing queue editor....................................................... 137
Open batch reprocessing queue editor window ....................... 137
Batch reprocessing queue editor functions............................... 138
Print batch reprocessing queue................................................. 138
Close batch reprocessing queue editor .................................... 138
5 Notes – Maintenance – Faults
5.1 Practical notes on ion chromatography ........................................................ 139
5.1.1 Separating columns ........................................................................... 139
5.1.2 High-pressure pump .......................................................................... 140
5.1.3 Eluents ................................................................................................ 141
5.1.4 Peristaltic pump.................................................................................. 141
5.1.5 Suppressor module............................................................................ 142
5.1.6 Connections ....................................................................................... 142
...................................................
139
5.2 Maintenance and servicing .............................................................................. 143
5.2.1 General information............................................................................ 143
5.2.2 Passivation ......................................................................................... 143
5.2.3 Recycling ............................................................................................ 144
5.2.4 Shutdown............................................................................................ 144
5.2.5 Changing separating columns........................................................... 144
5.2.6 Maintenance work at the pump head ................................................ 146
5.2.7 Regeneration of the suppressor module ........................................... 150
5.2.8 Cleaning the suppressor.................................................................... 151
5.2.9 Replacing the suppressor.................................................................. 153
5.2.10 Exchanging the pump tubing............................................................. 155
5.3 Faults and malfunctions ................................................................................... 156
5.3.1 Error messages .................................................................................. 156
5.3.2 Malfunctions and their rectification .................................................... 156
5.4 Diagnostic tests / Validation / GLP ................................................................. 158
IV
792 Basic IC
Table of contents
6 Appendix
6.1 Technical data..................................................................................................... 159
6.2 Standard equipment ..........................................................................................164
6.3 Optional accessories.........................................................................................167
6.4 Warranty and conformity .................................................................................. 176
..............................................................................................................
6.1.1 Conductivity measurement................................................................. 159
6.1.2 Conductivity detector.......................................................................... 159
6.1.3 Injection valve ..................................................................................... 160
6.1.4 High-pressure pump .......................................................................... 160
6.1.5 Peristaltic pump.................................................................................. 161
6.1.6 Suppressor module ............................................................................ 161
6.1.7 RS232 interface .................................................................................. 161
6.1.8 Mains connection ............................................................................... 162
6.1.9 Safety specifications........................................................................... 162
6.1.10 Electromagnetic compatibility (EMC)................................................. 162
6.1.11 Ambient temperature.......................................................................... 163
6.1.12 Housing............................................................................................... 163
6.3.1 Accessories for 792 Basic IC .............................................................167
6.3.2 Separating columns and precolumns................................................ 169
6.3.3 Additional devices and cables ........................................................... 175
6.4.1 Warranty.............................................................................................. 176
6.4.2 EU Declaration of conformity.............................................................. 177
6.4.3 Certificate of conformity and system validation ................................. 178
159
6.4 Index ..................................................................................................................... 179
792 Basic IC
V
Table of contents
List of figures
Fig. 1: Front of the 792 Basic IC ................................................................................ 3
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
: Rear of the 792 Basic IC................................................................................. 4
: Interior of the 792 Basic IC .............................................................................6
: Connecting diagram for 792 Basic IC without suppressor.......................... 12
: Connecting diagram for 792 Basic IC with suppressor ............................... 12
: Connectors for capillaries.............................................................................15
: 6.2821.100 Filter unit PEEK .......................................................................... 16
: Setting the mains voltage ............................................................................. 18
: Connection of the pulsation dampener (option) .......................................... 24
Fig. 10
Fig. 11
Fig. 12
Fig. 13
Fig. 14
Fig. 15
Fig. 16
Fig. 17
Fig. 18
Fig. 19
: Installing precolumn cartridges .................................................................... 29
: Installation of precolumn glass cartridges with cartridge holder ................. 31
: Installation of column without suppressor.................................................... 33
: Installing pump tubings ................................................................................ 36
: Installation of column with suppressor......................................................... 37
: Connections at suppressor module ............................................................. 40
: Components of the pump head ................................................................. 147
: Replacement of the piston seal 89
: Components of inlet valve 90
: Assembling the suppressor.......................................................................
89............................................................. 147
8989
90 and outlet valve 91
9090
91 ..................................... 149
9191
153
VI
792 Basic IC
Table of contents
List of numbered parts and controls
1111 Door to interior ....................................3,6
2222 Mains pilot lamp.....................................3
3333 Feedthrough for syringe tubing .............3
4444 Feedthrough for aspirating tubing......... 3
5555 Opening for detector cable....................4
6666 Rear panel opening ...............................4
7777 Opening for outlet capillaries.................4
8888 Knurled screw ........................................4
9999 Detachable rear panel ...........................4
10
10 Transport security screws...................... 4
1010
11
11 Mains switch ..................................... 4,18
1111
12
12 Mains connection plug .....................4,18
1212
13
13 Fuse holder ....................................... 4,18
1313
14
14 RS232 interface...................................... 4
1414
15
15 Connection for detector block ............... 4
1515
16
16 Connection for service...........................4
1616
17
17 Serial number.........................................4
1717
18
18 Inlet capillary for injector ...................6,24
1818
19
19 Mounting rail ................................ 6,33,37
1919
20
20 Column connection
2020
capillary ..............................6,29,31,33,37
21
21 Sample loop...........................................6
2121
22
22 Injection valve..........................6,24,33,37
2222
23
23 Aspirating tubing....................................6
2323
24
24 Coupling................................................. 6
2424
25
25 Syringe tubing ........................................ 6
2525
26
26 PEEK coupling ............................. 6,29,37
2626
27
27 Connection capillary .........................6,24
2727
28
28 Filter unit PEEK ..................6,16,24,36,37
2828
29
29 Connection capillary .........................6,24
2929
30
30 Connection capillary ..............................6
3030
31
31 Purge valve........................................6,24
3131
32
32 Aspirating capillary................................. 6
3232
33
33 Fastening screws...................................6
3333
34
34 Pump head......................................6,147
3434
35
35 Connection capillary .............................. 6
3535
36
36 Connection capillary .............................. 6
3636
37
37 Inlet capillary for
3737
detector block .............................. 6,33,37
38
38 Detector block.............................. 6,33,37
3838
39
39 Suppressor module ..........................6,37
3939
40
40 Tubing cartridge ...........................6,36,37
4040
41
41 Contact pressure lever.................6,36,37
4141
42
42 Holding clamp.................................. 6,37
4242
43
43 Snap-action lever .........................6,36,37
4343
44
44 Pump drive ........................................6,37
4444
45
45 Mounting pin .....................................6,37
4545
46
46 Compression fitting ......... 15,16,29,31,36
4646
47
47 Compression fitting ..............................15
4747
48
48 Capillary......................................15,16,31
4848
49
49 Connector with filter..............................16
4949
50
50 Housing for filter unit PEEK..................16
5050
51
51 Connector without filter ........................16
5151
52
52 Pulsation dampener .............................24
5252
53
53 Connection to injection valve...............24
5353
54
54 Connection to purge valve...................24
5454
55
55 Inlet capillary.........................................29
5555
56
56 Manufit housing....................................29
5656
57
57 PTFE gasket .........................................29
5757
58
58 2 Steel meshes.....................................29
5858
59
59 Precolumn cartridge.............................29
5959
60
60 Manufit pressure screw........................29
6060
61
61 Outlet capillary......................................29
6161
62
62 End fitting .............................................31
6262
63
63 Sleeve for precolumn cartridge............31
6363
64
64 Precolumn cartridge.............................31
6464
65
65 Connection piece .................................31
6565
66
66 Separating column.....................29,33,37
6666
67
67 Column holder.................................33,37
6767
68
68 Aspirating tubing for H
6868
69
69 Aspirating tubing for H
6969
70
70 Coupling ..........................................36,37
7070
71
71 Pump tubing for H
7171
72
72 Pump tubing for H
7272
73
73 Stopper................................................ 36
7373
74
74 Coupling ......................................... 36,37
7474
75
75 Suppressor inlet capillary
7575
O ................36,37
2
.............36,37
2SO4
...................36,37
2SO4
O.......................36,37
2
for eluent......................................... 37,40
76
76 Suppressor outlet capillary
7676
for eluent......................................... 37,40
77
77 Suppressor inlet capillary
7777
for H
O....................................... 36,37,40
2
792 Basic IC
VII
Table of contents
78
78 Suppressor inlet capillary
7878
for H
79
79 Suppressor outlet capillary
7979
for H
80
80 Suppressor outlet capillary
8080
for H
81
81 Screw ................................................. 147
8181
82
82 Zircon piston ...................................... 147
8282
83
83 Spring retainer ................................... 147
8383
84
84 Spring................................................. 147
8484
85
85 Piston cartridge.................................. 147
8585
86
86 Piston guide sleeve............................147
8686
87
87 Sapphire supporting ring................... 147
8787
88
88 Piston guide sleeve............................147
8888
89
89 Piston seal .........................................147
8989
90
90 Inlet valve ....................................147,149
9090
2SO4
2
2SO4
....................................36,37,40
O ............................................37,40
.........................................37,40
91
91 Outlet valve..................................147,149
9191
92
92 Screw holder ......................................147
9292
93
93 Special tool ........................................147
9393
94
94 Special tool ........................................147
9494
95
95 Valve housing.....................................149
9595
96
96 Sealing ring ........................................149
9696
97
97 Sleeve.................................................149
9797
98
98 Sapphire sleeve .................................149
9898
99
99 Sapphire sphere.................................149
9999
100
100 Ceramic holder...................................149
100100
101
101 Seal ....................................................149
101101
102
102 Screw nut ...........................................153
102102
103
103 Connection piece...............................153
103103
104
104 Suppressor rotor ................................153
104104
105
105 Suppressor holder .............................153
105105
VIII
792 Basic IC
1.1 Instrument description
1 Introduction
1.1 Instrument description
2.792.0020 Basic IC with suppressor module
The
system for ion chromatographic analyses. The extremely compact
housing of the 792 Basic IC contains everything needed to carry out ion
chromatography at a high quality level:
is a PC-controlled
x Injection valve
x High-pressure pump
pump with a flow range from 0.2 } 2.5 mL/min and a maximum
pressure of 25 MPa (250 bar)
x Column chamber
not only thermally stable conditions for the separation column but
also shields the system against electromagnetic interference
x Columns
separation columns for cations or organic acids – the 792 Basic IC
can accommodate them all
x Suppressor
pressure-resistant, with fully automatic regeneration, highest performance and optimal reproducibility
x Peristaltic pump
flow rate of 0.5 } 0.6 mL/min for regeneration and rinsing of the
suppressor module
x Detector
stability. The detector temperature of 40 °C varies by less than
0.01°C.
– conductivity detector with outstanding temperature
– for individual injections
– extremely low-pulsation double piston
– the perfect insulation of the housing provides
– whether anion columns with or without suppression,
– integrated Metrohm Suppressor Module (MSM),
– integrated two-channel peristaltic pump with a
792 Basic IC
All components, which come into contact with eluent and sample are
metal-free.
operation of the
The
the RS232 interface with the help of the control and evaluation program
«792 Basic IC»
tems for recording and evaluating chromatograms and modify them if
need be. Time programs can also be created in which a large number
of instrument functions can be triggered for each program step.
792 Basic IC takes place via a PC connected to
. This PC program can be used to open prepared sys-
1
1 Introduction
The operating software for the 792 Basic IC meets all the requirements
you could place today on a modern integration software: single or multipoint calibration, internal or external standard, selectable algorithms for
non-linear calibration, various integration modes with integration parameters and integration events, different methods for peak recognition,
peak editor, free scaling, superimposing several chromatograms, batch
reprocessing; a powerful and GLP-conform report generator with output
interfaces for monitor, printer and external databases.
792 Basic IC
2
1.2 Parts and controls
1.2 Parts and controls
Basic I C792
Basic
Basic
BasicBasic
POWER
222211113
Fig. 1
: Front of the 792 Basic IC
34
33
4
44
Door to interior
1111
Mains pilot lamp
2222
lit up when instrument switched on
792 Basic IC
3333 Feedthrough for syringe tubing
for connection of the 6.2816.020 syringe
used für aspirating the sample
Feedthrough for aspirating tubing
4444
3
1 Introduction
8888
8888
17
17
1717
5555
Type
WARNING - Fire Hazard -
For conti nued protectio n replace only
with the sam e type and rating of fuse
66667
76
77
Waste B
Waste A
65
66
Transport security screws
5
55
8888
9999
8888
10
10
1010
16
16
1616
Service only
Detector Block
15
15 14
1515
RS 232
14
1414
: Rear of the 792 Basic IC
Fig. 2
f = 50-60 H z
S = 100 VA
Fuse
100-120V :
1,0A(T)
220-240V :
0,5A(T)
Made by Metrohm Herisau Switzerland
11
11
1111
12
12
1212
13
13
1313
792 Basic IC
4
1.2 Parts and controls
Opening for detector cable
5555
Opening for outlet capillaries
6666
for discharge of eluent, regeneration
solution, and rinsing solution from the
inner compartment
Rear panel opening
7777
(closed with plastic stopper) for
additional supply and discharge lines to
and from the inner compartment
Knurled screw
8888
for fastening the rear panel
Detachable rear panel
9999
access to upper part of inner
compartment
10
10 Transport security screws
1010
to secure the pump head when the
instrument is transported
9999
12
12 Mains connection plug
1212
mains connection, see
13
13 Fuse holder
1313
changing the fuses, see
RS232 interface
14
14
1414
connection of the PC
15
15 Connection for detector block
1515
Connection
16
16
1616
To be used only for Metrohm Service!
Serial number
17
17
1717
section 2.4
section 2.4
Mains switch
11
11
1111
to switch instrument on and off:
I = ON 0 = OFF
792 Basic IC
5
1 Introduction
38
38
3838
37
37
3737
36
36
3636
35
35
3535
34
34
3434
33
33
3333
32
32
3232
18
18
1818
19
19
1919
20
20
2020
21
21
2121
39
39
3939
1111
31
31
3131
30
30
3030
POWER
29
29
28
28 27
2929
Fig. 3: Interior of the 792 Basic IC
27
2828
2727
(with permanently attached accessories and
1.733.0110 Detector block)
26
26
2626
45
45 44
44
4545
4444
25
25
2525
43
4343
42
4243
4242
19
19
1919
22
22
2222
40
40
4040
41
41
4141
23
23
2323
24
24
2424
792 Basic IC
6
1.2 Parts and controls
Door to interior
1111
Inlet capillary for injector
18
18
1818
PEEK capillary,
length L = 24 cm
19
19 Mounting rail
1919
for 6.2027.0X0 column holder
Column connection capillary
20
20
2020
PEEK capillary,
length L = 30 cm
Sample loop 20 PPPPL
21
21
2121
6.1825.210 PEEK sample loop
Injection valve
22
22
2222
Aspirating tubing
23
23
2323
PTFE tubing for aspirating the sample;
length L = 65 cm
Aspirating capillary
32
32
3232
Connection for 6.1834.010 aspirating
tubing
Fastening screws
33
33
3333
for pump head
Pump head (6.2824.100)
34
34
3434
35
35 Connection capillary
3535
Connection pump head – purge
valve, fixed mounting
36
36 Connection capillary
3636
in pump head, fixed mounting
37
37 Inlet capillary for detector block
3737
PEEK capillary, fixed mounting
38
38 Detector block (1.732.0110)
3838
34
34
3434
Coupling (6.2744.120)
24
24
2424
for connection of the 6.2816.020 syringe
Syringe tubing
25
25
2525
PTFE tubing for connection of the
syringe,
length L = 35 cm
26
26 PEEK coupling (6.2744.040)
2626
27
27 Connection capillary
2727
PEEK capillary,
length L = 13 cm
28
28 Filter unit PEEK (6.2821.100) 43
2828
Connection capillary
29
29
2929
PEEK capillary,
length L = 13 cm
30
30 Connection capillary
3030
PTFE capillary for connection of the
6.2816.020 syringe,
length L = 70 cm
39
39 Suppressor module
3939
40
40 Tubing cartridge (6.2755.000)
4040
41
41 Contact pressure lever
4141
42
42 Holding clamp
4242
43 Snap-action lever
4343
44
44 Pump drive
4444
44445555 Mounting pin
(inlet and outlet capillaries are not
shown)
for 6.1826.060 pump tubing
for adjusting the contact pressure
for locking the tubing cartridge into
place
for releasing the tubing cartridge
roller head with contact rollers
for attaching the tubing cartridges
Purge valve
31
31
3131
792 Basic IC
7
1 Introduction
1.3 Information on the Instructions for Use
Please read through these Instructions for Use carefully before you put
the 792 Basic IC into operation. The Instructions for Use contain
information and warnings to which the user must pay attention in order
to assure safe operation of the instrument.
1.3.1 Organization
These
comprehensive overview of the installation, startup procedure, operation, fault rectification and technical specifications of this instrument.
The Instructions for Use are organized as follows:
To find the required information on the instruments, you will find it an
advantage to use either the
back.
8.792.1003 Instructions for Use
Section 1 Introduction
General description of instrument, parts and controls
and safety notes
Section 2 Installation
Installation of accessories and putting instrument into
operation
Section 3 Operating tutorial
Introduction to the operation using an example
Section 4 Operation
Detailed description of the operation
Section 5 Notes – Maintenance – Faults
Notes on ion chromatography, maintenance, fault rectification, diagnostic tests, validation
Section 6 Appendix
Technical data, standard equipment, options, warranty,
declarations of conformity, index
Table of contents
for the 792 Basic IC provide a
or the
Index
at the
As a supplement to the Instructions for Use, the
Monograph "Ion chromatography"
an introduction to the theoretical fundamentals and general information
on separating columns and sample pretreatment.
8.792.5003 Metrohm Monograph "Practical Ion Chromatog-
The
raphy"
tion to the basic principles of ion chromatography and also describes
22 experiments covering the whole world of ion chromatography.
The
tains all the
792 Basic IC
8
is a practical textbook, which provides an illustrative introduc-
8.732.2013 IC Applications Collection
Application Notes
on the subject of ion chromatography.
is also supplied. This provides
8.732.2003 Metrohm
is also supplied; this con-
1.3 Information on the Instructions for Use
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these Instructions for Use:
Range
SYSTEM STATE
<OK>
[ Ctrl ]
35
35
3535
Menu item, parameter or entry
value
Program window
Button
Key
Part or control of 792
Hazard
This symbol draws attention to a
possible danger to life or of injury if
the associated directions are not
followed correctly.
Warning
This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed correctly.
792 Basic IC
Caution
This symbol marks important
information. First read the associated directions before you continue.
Comment
This symbol marks additional
information and tips.
9
1 Introduction
1.4 Safety notes
1.4.1 Electrical safety
While electrical safety in the handling of the 792 Basic IC is assured in
the context of the specifications IEC 1010-1 (protection class 1, degree
of protection IP20), the following points should be noted:
x Mains connection
Setting of the mains voltage, checking the mains fuse and the
mains connection must be effected in accordance with the instruc-
tions in section 2.4.
x Opening the 792 Basic IC
If the 792 Basic IC is connected to the power supply, the instrument
must not be opened nor must parts be removed from it, otherwise
there is a danger of coming into contact with components which are
live. Hence, always disconnect the instrument from all voltage sources
before you open it and ensure that the mains cable is discon-
nected from mains connection 12
x Protection against static charges
Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 792 Basic IC, you should earth yourself and any tools you
are using by touching an earthed object (e.g. housing of the instrument or a radiator) to eliminate any static charges which exist..
12 !
1212
1.4.2 General precautionary rules
x Handling of solvents
Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.
792 Basic IC
10
2.1 Overview
2 Installation
2.1 Overview
2.1.1 Flow chart
The following flow chart provides an overview of all installation work. You
will find more detailed information in the relevant sections.
Setting up
Setting up sect. 2.2
Setting upSetting up
Installing accessories
Installing accessories sect. 2.3
Installing accessoriesInstalling accessories
Mains connection
Mains connection sect. 2.4
Mains connectionMains connection
Connecting PC
Connecting PC sect. 2.5
Connecting PCConnecting PC
sect. 2.2
sect. 2.2sect. 2.2
sect. 2.3
sect. 2.3sect. 2.3
sect. 2.4
sect. 2.4sect. 2.4
sect. 2.5
sect. 2.5sect. 2.5
Connecting high pr. pump
Connecting high pr. pump sect 2.6
Connecting high pr. pumpConnecting high pr. pump
Precolumn
Precolumn
PrecolumnPrecolumn
No
No
NoNo
Installing sample loop
Installing sample loop sect. 2.7.6
Installing sample loopInstalling sample loop
Connecting column
Connecting column sect. 2.7.7/8
Connecting columnConnecting column
Suppressor
Suppressor Connecting suppressor module
SuppressorSuppressor
No
No
NoNo
Conditioning
Conditioning sect. 2.9
ConditioningConditioning
sect 2.6
sect 2.6sect 2.6
Yes
Yes
YesYes
sect. 2.7.6
sect. 2.7.6sect. 2.7.6
sect. 2.7.7/8
sect. 2.7.7/8sect. 2.7.7/8
Yes
Yes
YesYes
sect. 2.9
sect. 2.9sect. 2.9
Precolumn with twin cartridge holder
Precolumn with twin cartridge holder sect. 2.7.2
Precolumn with twin cartridge holderPrecolumn with twin cartridge holder
Precolumn cat. with cartr. holder
Precolumn cat. with cartr. holder sect. 2.7.3
Precolumn cat. with cartr. holderPrecolumn cat. with cartr. holder
IC anion precolumn SUPERSEP
IC anion precolumn SUPERSEP sect. 2.7.4
IC anion precolumn SUPERSEPIC anion precolumn SUPERSEP
Connecting suppressor module sect. 2.8
Connecting suppressor moduleConnecting suppressor module
sect. 2.7.2
sect. 2.7.2sect. 2.7.2
sect. 2.7.3
sect. 2.7.3sect. 2.7.3
sect. 2.7.4
sect. 2.7.4sect. 2.7.4
sect. 2.8
sect. 2.8sect. 2.8
2.1.2 Connections in the 792 Basic IC
The two following illustrations show the internal connections in the 792
Basic IC in schematic form. The meanings of the various numbered
components are given in the detailed illustrations and descriptions in
ections 2.2 – 2.9
s
792 Basic IC
.
11
2 Installation
20
32
32
3232
31
31
3131
34
34
3434
29
29
2929
36
36 35
3636
35
3535
28
28 27
27
2828
2727
18
18 21
21 22
1818
2121
20 66
2020
22
2222
Injection valve
Column
23
23
2323
66 37
6666
37 38
3737
38
3838
Detector
6.1834.010
32
32
3232
30
30
3030
6.2821.090
Eluent
Fig. 4:
36
36 35
3636
34
34
3434
Connecting diagram for 792 Basic IC without suppressor
35
3535
28
28
2828
52
52
5252
18
18 21
1818
27
27
2727
24
24
2424
21 22
2121
22
2222
20
20 66
2020
78
78
7878
25
25
2525
Sample
66 75
6666
Column
80
80
8080
Waste
75
7575
76
76 26
26 37
7676
2626
39
39
3939
37 38
3737
Detector
77
77
7777
79
79
7979
38
3838
29
29
2929
28
28
H2O
68
68
6868
2828
72
7272
74
74
7474
31
31
3131
6.1834.010
12
30
30
3030
6.2821.090
Eluent
792 Basic IC
: Connecting diagram for 792 Basic IC with suppressor
Fig. 5
52
52
5252
25
25
2525
24
24
2424
Sample
69
69
6969
23
23
2323
71
71
7171
70
70 72
7070
H2SO
4
Waste
2.2 Setting up the instrument
2.2 Setting up the instrument
2.2.1 Packaging
The 792 Basic IC is supplied together with the separately packed
accessories in special packagings containing shock-absorbing foam
linings designed to provide excellent protection. The instrument itself is
packed in an evacuated polyethylene bag to prevent the ingress of
dust. Please store all these special packagings as only they assure
transport of the instrument free from damage.
2.2.2 Check
After receipt, immediately check whether the shipment is complete and
has arrived without damage (compare with delivery note and list of
accessories in
instructions in
2.2.3 Location
section 6.2
section 6.4.1
). In the case of transport damage, see
"Warranty".
Position the instrument in the laboratory at a location convenient for operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals.
To avoid disturbing temperature influences on the insulated column
compartment, the instrument must be protected against direct
sunlight.
2.3 Attaching the accessories
2.3.1 Connection of detector block
The metal-free
livery of the 792 Basic IC; it must be inserted in the instrument and connected up. Proceed as follows:
1 Note the cell constant
x
1.732.0110 Detector block
The cell constant
detector block. Note this value; it must subsequently be entered in the software in order to ensure that an exact display
of the conductivity is obtained (see
c = XX,X /cm
belongs to the scope of de-
is printed on the rear of the
section 2.5.3
).
792 Basic IC
2 Install detector block
x
Unscrew the four knurled screws
of the 792 Basic IC and remove rear panel (see
x
Position detector block
vided in the 792 Basic IC and push fully to the front (see
3
).
8888
from the top rear panel
Fig. 2
38
38
from the back in the space pro-
3838
).
9999
Fig.
13
2 Installation
x
Insert the cable permanently attached to the detector block
38
38
in one of the openings
3838
the openings
x
Replace rear panel
four knurled screws
3 Connect detector block
x
Plug the gray connecting cable permanently attached to the
detector block
792 Basic IC and fasten to the instrument by tightening the
screws in the cable connector (see
4 Connect waste container
x
Lead the outlet capillary of the detector block
ciently large waste container and fix in place.
7777
of the rear panel
9999
38
38
into connection
3838
5555
and the outlet capillary in one of
9999
.
and screw to the 792 Basic IC using the
8888
.
15
15
"Detector Block" of the
1515
Fig. 2
).
38
38
to a suffi-
3838
2.3.2 Connection of syringe and aspirating tubing
22
For manual filling of the sample loop
the 6.2816.020 Syringe, the syringe tubing
23
PTFE aspirating tubing
as follows:
1 Connect syringe to syringe tubing
x
Pull syringe tubing
hand out of feedthrough
x
Mount a PEEK compression fitting
the end of the syringe tubing
(6.2744.120) to the compression fitting
x
Push 6.2816.020 Syringe (without needle) as far as it will go
into the connection of coupling
2 Install aspirating tubing
x
Pull aspirating tubing
hand out of feedthrough
23
are needed. These accessories are mounted
2323
25
25
2525
22
mounted on the injection valve,
2222
25
25
with coupling
2525
connected to injection valve
3333
as far as desired (see
46
46
(see
4646
25
25
and screw the coupling
2525
24
24
.
2424
23
23
connected to injection valve
2323
4444
as far as desired (see
section 2.3.4
46
46
.
4646
24
24
2424
22
22
2222
Fig. 3
Fig. 3
and the
by
).
) to
24
24
2424
22
22
by
2222
).
2.3.3 Connection of the 6.5324.000 Bottle rack (option)
The optional available 6.5324.000 Bottle rack for supply vessels can be
placed on top of the 792 Basic IC. The accessories include the supply
vessels for eluent (2 L), regeneration solution (1 L) and rinsing solution
(1 L).
792 Basic IC
14
2.3 Attaching the accessories
2.3.4 Connection of PEEK capillaries
For the connections between high-pressure pump and detector block
6.1831.010 PEEK capillaries
which are connected using either
tings (long)
These PEEK connectors can also be used to connect 6.1822.010 PTFE
microcapillaries (i.d. = 0.3 mm). Proceed as follows:
Capillaries fitted with new connectors must have a perfectly flat cut
surface. To cut PEEK or PTFE capillaries it is best to use the
6.2621.080 Capillary tubing cutter.
1 Mount compression fitting
Slide a compression fitting
fitting
fastened as shown in
2 Insert capillary in connection
Push capillary end in the corresponding connection as far as it
will go (to avoid dead volume).
6.2744.070 PEEK compression fittings (short)
or
47
47
(6.2744.070) over the end of the capillary
4747
(i.d. = 0.25 mm, e.d. =
6.2744.010 PEEK compression fit-
46
46
(6.2744.010) or a compression
4646
Fig. 6
.
1
16
"
/
) are used
48
48
to be
4848
.
46
46 48
4646
Fig. 6
: Connectors for capillaries
48 47
4848
3 Tighten compression fitting
46
46
47
Tighten compression fitting
47
4747
46
46
4646
47
47
4747
48
48
4848
47
or
4646
4747
Compression fitting
(6.2744.010)
Compression fitting
(6.2744.070)
Capillary
6.1831.010 PEEK capillary or
6.1822.010 PTFE microcapillary
by hand (never use tools).
792 Basic IC
15
2 Installation
2.3.5 Filter unit PEEK
6.2821.100 Filter unit PEEK
One
tween the high-pressure pump and the injection valve
sic IC. This filter unit serves to avoid contamination by abrasive particles
of the piston seals.
The two other filter units PEEK supplied with the 792 Basic IC are installed between the pump tubings of the peristaltic pump and the inlet
capillaries for regeneration and rinsing solution (see
These filter units serve to protect the suppressor module from foreign
particles and bacterial growth.
28
The Filter unit PEEK
49
49
tors
For the connection of capillaries
(6.2744.010) or
filter are available as an option with the ordering number 6.2821.110
(set of 10).
(with filter) and
4949
For the connection of the filter unit, please note the flow direction
arrow printed on the housing.
28
consists of the housing
2828
51
51
(without filter) screwed into the housing
5151
47
47
(6.2744.070) must be used. New connectors
4747
28
28
2828
Fig. 7
(see
48
48
, PEEK compression fittings
4848
) is already installed be-
22
22
at the 792 Ba-
2222
section 2.8.2
50
50
and the two connec-
5050
49
49
4949
).
50
50
5050
46
46
4646
with
.
48
48 46
4848
Filter unit PEEK (6.2821.100)
28
28
2828
Compression fitting (6.2744.010)
46
46
4646
Capillary
48
48
4848
6.1831.010 PEEK capillary or
6.1822.010 PTFE microcapillary
792 Basic IC
16
46 49
4646
49 50
4949
: 6.2821.100 Filter unit PEEK
Fig. 7
50 51
5050
Connector with filter (6.2821.110)
49
49
4949
Part of 6.2821.100 Filter unit
50
Housing for filter unit
50
5050
Part of 6.2821.100 Filter unit
Connector without filter
51
51
5151
Part of 6.2821.100 Filter unit
51 46
5151
46 48
4646
48
4848
2.4 Mains connection
2.4 Mains connection
Follow the instructions below for connecting to the power supply. If
the instrument is operated with a mains voltage set wrongly and/or
wrong mains fuse, there is a danger of fire!
2.4.1 Setting the mains voltage
Before switching on the 792 Basic IC for the first time, check that the
mains voltage set on the instrument (see
mains voltage. If this is not
on the instrument as follows:
1 Disconnect mains cable
Disconnect mains cable from mains connection plug
792 Basic IC.
the case, you must reset the mains voltage
Fig. 8
) matches the local
12
12
1212
of the
2 Remove fuse holder
13
Using a screwdriver, loosen fuse holder
12
connection plug
3 Check and change fuse if necessary
Carefully take the fuse installed for the desired mains voltage out
of fuse holder
fuse in the fuse holder is marked by the white arrow imprinted
next to the mains voltage range):
100}}}}120 V 1.0 A (slow-blow)
220}}}}240 V 0.5 A (slow-blow)
4 Insert fuse
Change fuse if necessary and reinsert in fuse holder
5 Install fuse holder
Depending on the desired mains voltage, insert fuse holder
the 792 Basic IC so that the corresponding mains voltage range
can be read normally and the adjacent white arrow points to the
white bar imprinted below the fuse holder (see
12
and take out completely.
1212
13
13
and check its specifications (the position of the
1313
13
below the mains
1313
Metrohm No. U.600.0016
Metrohm No. U.600.0013
13
13
1313
Fig. 8
).
.
13
13
in
1313
792 Basic IC
17
2 Installation
2.4.2 Fuses
100 – 120 V
Mains switch
11
11
1111
Mains connec-
12
12
1212
11
11
1111
12
12
1212
220 – 240 V
tion plug
220
240 V
100
----
120 V
----
13
13 Fuse holder
1313
13
13
1313
220
----
240 V
100
120 V
----
Fig. 8
: Setting the mains voltage
One of the two fuses 1 A/slow-blow for 100}120 V or 0.5 A/slow-blow
13
for 220}240 V is installed in fuse holder
13
of the 792 Basic IC as
1313
standard.
Ensure that the instrument is never put into operation with fuses of
another type, otherwise there is danger of fire!
For checking or changing fuses, process as described in
2.4.3 Mains cable and mains connection
Mains cable
The instrument is supplied with one of three mains cables
x
6.2122.020 with plug SEV 12 (Switzerland, })
x
6.2122.040 with plug CEE(7), VII (Germany, })
x
6.2133.070 with plug NEMA 5-15 (USA, })
which are three-cored and fitted with a plug with an earthing pin. If a different plug has to be fitted, the yellow/green lead (IEC standard) must
be connected to protective earth (protection class 1).
Any break in the earthing inside or outside the instrument can make it
a hazard!
Mains connection
Plug the mains cable into mains connection plug
Fig. 8
(see
).
section 2.4.1
12
12
of the 792 Basic IC
1212
.
2.4.4 On/off switching of the instrument
The 792 Basic IC is switched on and off using mains switch
the instrument is switched on, the mains pilot lamp
792 Basic IC
18
2222
lights up.
11
11
. When
1111
2.5 Connection to the PC
2.5 Connection to the PC
2.5.1 Connecting cable
Always switch off 792 Basic IC and PC before you connect the two
instruments with the 6.2134.100 Cable.
14
Connect the RS232 interface
COM ports at the PC using the 6.2134.100 Cable (9 pin/9 pin). If only a
25-pin COM interface is available on the PC then the optionally available 6.2125.110 Cable (9 pin/25 pin) or a commercially available
adapter must be used.
2.5.2 Software installation
14
at the 792 Basic IC to one of the serial
1414
The PC program
«792 Basic IC 1.0»
is required for the operation of
the 792 Basic IC; this is contained on the 6.6045.003 CD included in the
accessories. This program runs under Windows 95, Windows 98, Windows NT and Windows 2000 operating systems and is installed as follows:
1 Install program
x
Insert 6.6045.003 Installation CD into CD drive.
x
Select
<Start>
installation CD and click on
x
Click on "
792
and
Run
. Browse for the
<OK>
.
setup.exe
file on the
" and follow the instructions given in the setup
program.
The software package will be installed in the desired direc-
tory. In addition to the program files, the following folders are
installed:
Data
Folder for storage of chromatogram files
Devices
Methods
Reports
Systems
*.chw
) and batch reprocessing files (
(
Folder for storage of device files (
Folder for storage of method files (
Folder for storage of report files (
*.wmf
and graphic files (
)
Folder with subfolders with system files
*.smt
).
(
*.dev
*.mtw
*.txt
*.bar
)
)
)
)
792 Basic IC
2 Registrierung
x
Please send us your 8.792.8007 Registration card as soon as
possible. Only registered users will get updated program versions at a special price.
19
2 Installation
2.5.3 Basic settings
When the program is started for the first time several basic settings
must be made for the 792 Basic IC. Proceed as follows:
1 Start program
x
Double-click the software icon to start the program. The
program window with the opening picture is opened and the
Log In window appears on the screen:
x
Do not
enter any password here, just click on
<Log In>
following window appears:
x
Enter company, division and name and click on
<OK>
window appears only one time after software installation.
2 COM port settings
This step must only be carried out if a different COM interface
from COM1 is used for connection to the 792 Basic IC.
x
Click on
Options / 792 Basic IC:COM1
to open the
Links
. The
. This
window:
792 Basic IC
20
2.5 Connection to the PC
x
Click on
Change
COM1
menu item to open a window listing all available COM
ports at the PC.
x
Select the desired COM port to which the 792 Basic IC has
been connected and click on
x
Close the
Links
3 Open a system
x
Click on
folder
<Open>
File / Open / System
Default
. Select the system file
. The corresponding system window is opened:
using the right mouse button and select the
<OK>
. The window is closed.
window by clicking on
<OK>
.
in the main window. Open the
Default.smt
and click on
792 Basic IC
x
If the connection between PC and 792 Basic IC is working the
message
'#####' not found! Create?
create the configuration file
x
If the connection between PC and Personal IC does not
then the message
COM# ]]
Hardware settings file for 792 unit with serial number
will appear. Click
#####.792
Detection of hardware failed[792 Basic IC [
appears in the
SYSTEM STATE
<Yes>
in order to
for this instrument.
window. In this case
check whether the instrument has been switched on, whether
the connection cable is connected up properly and whether
the COM interface has been set correctly (see point 2). Then
repeat point 3.
work
21
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