Olympus AU 400 - 600 - 640 instructions
rev 422.0.2 - 2010-08-01
Chema Diagnostica
Via Campania 2/4 I-60030 Monsano | An Italy
T +39 0731 605064 | F +39 0731 605672 www.chema.com | mail@chema.com
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Olympus AU 400 - 600 - 640 instructions
rev 422.0.2 - 2010-08-01
Warning!
These programs should be used only as guideline. To check the reagent correct setting on the instrument, use only good quality control sera and work accordingly to with good laboratory practice. Instrumental factor must be checked. Chema Diagnostica will not be responsible for instrumental bad programming.
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 3 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
ACID PHOSPHATASE - Codes AC 0120 TC |
Preparation: |
POWDER SINGLE REAGENT. FOLLOW PREPARATION PROGRAM INDICATED IN INSERT |
|
SHEET. INSTALL AS R1 |
Storage: |
REFRIGERATE AT 2-8°C |
TEST NAME: |
ACP |
|
|
|
|
|
Sample: |
Volume |
20 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
|
Reagents: |
R1 volume |
200 μl |
Dilution |
0 μl |
|
|
|
R2 volume |
0 μl |
Dilution |
0 μl |
|
|
Wavelenght: |
Pri. 410 |
Sec. 800 |
Min OD |
|
Max OD |
|
Method: |
RATE |
|
L |
- 0.1 |
H |
2.5 |
Reaction slope: + |
|
Reagent OD limit: |
|
|
||
|
|
|
First L |
- 0.1 |
First H |
0.3 |
|
|
|
Last L |
- 0.1 |
First H |
0.3 |
Measuring point 1: First 12 |
Last 27 |
|
|
|
|
|
Measuring point 2: First |
Last |
|
|
|
|
|
Linearity: |
20% |
|
Dynamic range: |
|
|
|
No Lag Time: |
NO |
|
L 1 |
H 60 |
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 30
Unit: U/L
Factor: 853
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 4 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
ALBUMIN - Codes BC 0100 / 0500 / 1000 / 1500 CH |
Preparation: |
R1 - LIQUID READY TO USE SINGLE REAGENT |
Storage: |
REFRIGERATE (2-8°C) |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
ALB |
|
|
|
|
|
Sample: |
Volume |
2 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
|
Reagents: |
R1 volume |
295 μl |
Dilution |
0 μl |
|
|
|
R2 volume |
0 μl |
Dilution |
0 μl |
|
|
Wavelenght: |
Pri. 600 |
Sec. 800 |
Min OD |
|
Max OD |
|
Method: |
END |
|
L |
|
H |
|
Reaction slope: + |
|
Reagent OD limit: |
|
|
||
|
|
|
First L |
- 0.1 |
First H |
0.5 |
|
|
|
Last L |
- 0.1 |
First H |
0.5 |
Measuring point 1: First 0 |
Last 4 |
|
|
|
|
|
Measuring point 2: First |
Last |
|
|
|
|
|
Linearity: |
|
|
Dynamic range: |
|
|
|
No Lag Time: |
YES |
|
L 0.1 |
H 6.0 |
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 90
Unit: g/dl
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 5 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
ALKALINE PHOSPHATASE FL (IFCC) - Codes AF F080 / F245 / F400 / F600 CH |
Preparation: |
R1 - INSTALL LIQUID READY TO USE REAGENT A |
|
R2 - INSTALL LIQUID READY TO USE REAGENT B |
Storage: |
REFRIGERATE AT 2-8°C |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
ALP |
|
|
|
|
|
Sample: |
Volume |
5 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
|
Reagents: |
R1 volume |
200 μl |
Dilution |
0 μl |
|
|
|
R2 volume |
50 μl |
Dilution |
0 μl |
|
|
Wavelenght: |
Pri. 410 |
Sec. 480 |
Min OD |
|
Max OD |
|
Method: |
RATE |
|
L |
-0.1 |
H |
2.5 |
Reaction slope: + |
|
Reagent OD limit: |
|
|
||
|
|
|
First L |
-0.1 |
First H |
1.5 |
|
|
|
Last L |
-0.1 |
First H |
1.5 |
Measuring point 1: First 13 |
Last 19 |
|
|
|
|
|
Measuring point 2: First |
Last |
|
|
|
|
|
Linearity: |
20% |
|
Dynamic range: |
|
|
|
No Lag Time: |
NO |
|
L 5 |
H 3000 |
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 60
Unit: U/L
Factor: 2757
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 6 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
ALKALINE PHOSPHATASE (DGKC) FL - Codes AL F080 / F245 / F400 / F600 CH |
Preparation: |
R1 - INSTALL LIQUID READY TO USE REAGENT A |
|
R2 - INSTALL LIQUID READY TO USE REAGENT B |
Storage: |
REFRIGERATE AT 2-8°C |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
ALP |
|
|
|
|
|
Sample: |
Volume |
5 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
|
Reagents: |
R1 volume |
200 μl |
Dilution |
0 μl |
|
|
|
R2 volume |
50 μl |
Dilution |
0 μl |
|
|
Wavelenght: |
Pri. 410 |
Sec. 480 |
Min OD |
|
Max OD |
|
Method: |
RATE |
|
L |
- 0.1 |
H |
2.0 |
Reaction slope: + |
|
Reagent OD limit: |
|
|
||
|
|
|
First L |
- 0.1 |
First H |
1.2 |
|
|
|
Last L |
- 0.1 |
First H |
1.2 |
Measuring point 1: First 20 |
Last 24 |
|
|
|
|
|
Measuring point 2: First |
Last |
|
|
|
|
|
Linearity: |
20% |
|
Dynamic range: |
|
|
|
No Lag Time: |
NO |
|
L 7 |
H 3000 |
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 30
Unit: U/L
Factor: 2757
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 7 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
AMYLASE FL - Codes AM F060 / F120 / F245 CH |
Preparation: |
R1 - LIQUID READY TO USE SINGLE REAGENT |
Storage: |
REFRIGERATE AT 2-8°C |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
AMY |
|
|
|
|
|
Sample: |
Volume |
6 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
|
Reagents: |
R1 volume |
240 μl |
Dilution |
0 μl |
|
|
|
R2 volume |
0 μl |
Dilution |
0 μl |
|
|
Wavelenght: |
Pri. 410 |
Sec. 480 |
Min OD |
|
Max OD |
|
Method: |
RATE |
|
L |
- 0.1 |
H |
2.0 |
Reaction slope: + |
|
Reagent OD limit: |
|
|
||
|
|
|
First L |
- 0.1 |
First H |
0.3 |
|
|
|
Last L |
- 0.1 |
First H |
0.3 |
Measuring point 1: First 5 |
Last 10 |
|
|
|
|
|
Measuring point 2: First |
Last |
|
|
|
|
|
Linearity: |
20% |
|
Dynamic range: |
|
|
|
No Lag Time: |
NO |
|
L 10 |
H 3000 |
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 30
Unit: U/L
Factor: 3178
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 8 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
AMYLASE EPS FL - Codes EA F080 / F245 CH |
Preparation: |
R1 - INSTALL LIQUID READY TO USE REAGENT A |
|
R2 - INSTALL LIQUID READY TO USE REAGENT B |
Storage: |
REFRIGERATE AT 2-8°C |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
AMY |
|
|
|
|
|
Sample: |
Volume |
8 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
|
Reagents: |
R1 volume |
192 μl |
Dilution |
0 μl |
|
|
|
R2 volume |
48 μl |
Dilution |
0 μl |
|
|
Wavelenght: |
Pri. 410 |
Sec. 480 |
Min OD |
|
Max OD |
|
Method: |
RATE |
|
L |
- 0.1 |
H |
2.0 |
Reaction slope: + |
|
Reagent OD limit: |
|
|
||
|
|
|
First L |
- 0.1 |
First H |
0.3 |
|
|
|
Last L |
- 0.1 |
First H |
0.3 |
Measuring point 1: First 20 |
Last 27 |
|
|
|
|
|
Measuring point 2: First |
Last |
|
|
|
|
|
Linearity: |
20% |
|
Dynamic range: |
|
|
|
No Lag Time: |
NO |
|
L 6 |
H 1500 |
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 30
Unit: U/L
Factor: 3480
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 9 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
PANCREATIC ISOAMYLASE FL - Code PA F080 / F245 CH |
Preparation: |
R1 - INSTALL LIQUID READY TO USE REAGENT A |
|
R2 - INSTALL LIQUID READY TO USE REAGENT B |
Storage: |
REFRIGERATE AT 2-8°C |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
AMYP |
|
|
|
|
|
Sample: |
Volume |
5 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
|
Reagents: |
R1 volume |
200 μl |
Dilution |
0 μl |
|
|
|
R2 volume |
50 μl |
Dilution |
0 μl |
|
|
Wavelenght: |
Pri. 410 |
Sec. 480 |
Min OD |
|
Max OD |
|
Method: |
RATE |
|
L |
- 0.1 |
H |
2.0 |
Reaction slope: + |
|
Reagent OD limit: |
|
|
||
|
|
|
First L |
- 0.1 |
First H |
0.3 |
|
|
|
Last L |
- 0.1 |
First H |
0.3 |
Measuring point 1: First 20 |
Last 27 |
|
|
|
|
|
Measuring point 2: First |
Last |
|
|
|
|
|
Linearity: |
20% |
|
Dynamic range: |
|
|
|
No Lag Time: |
NO |
|
L 2 |
H 2500 |
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 30
Unit: U/L
Factor: 3480
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 10 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
BICARBONATE FL - Code BR F060 / F245 / F400 CH |
Preparation: |
R1 - LIQUID READY TO USE SINGLE REAGENT |
Storage: |
REFRIGERATE AT 2-8°C |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
CO2 |
|
|
|
|
|
Sample: |
Volume |
2.5 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
|
Reagents: |
R1 volume |
250 μl |
Dilution |
0 μl |
|
|
|
R2 volume |
0 μl |
Dilution |
0 μl |
|
|
Wavelenght: |
Pri. 410 |
Sec. 480 |
Min OD |
|
Max OD |
|
Method: |
FIXED |
|
L |
0.1 |
H |
2.5 |
Reaction slope: - |
|
Reagent OD limit: |
|
|
||
|
|
|
First L |
- 2.0 |
First H |
2.5 |
|
|
|
Last L |
- 2.0 |
First H |
2.5 |
Measuring point 1: First 1 |
Last 9 |
|
|
|
|
|
Measuring point 2: First |
Last |
|
|
|
|
|
Linearity: |
|
|
Dynamic range: |
|
|
|
No Lag Time: |
|
|
L 1 |
H 50 |
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 30
Unit: mmol/L
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 11 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
BILIRUBIN TOTAL FL - Code DT F125 / F500 CH |
Preparation: |
R1 - INSTALL LIQUID READY TO USE REAGENT A |
|
R2 - INSTALL LIQUID READY TO USE REAGENT B |
Storage: |
REFRIGERATE AT 2-8°C |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
BILT |
|
|
|
|
|
|
Sample: |
Volume |
10 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
||
Reagents: |
R1 volume |
200 μl |
Dilution |
0 μl |
|
|
|
|
R2 volume |
50 μl |
Dilution |
0 μl |
|
|
|
Wavelenght: |
Pri. 520 |
Sec. 800 |
Min OD |
|
Max OD |
|
|
Method: |
END |
|
|
L |
|
H |
|
Reaction slope: + |
|
|
Reagent OD limit: |
|
|
||
|
|
|
|
First L |
- 0.1 |
First H |
0.5 |
|
|
|
|
Last L |
- 0.1 |
First H |
0.5 |
Measuring point 1: First |
Last |
27 |
|
|
|
|
|
Measuring point 2: First |
Last |
10 |
|
|
|
|
|
Linearity: |
|
|
|
Dynamic range: |
|
|
|
No Lag Time: |
|
|
|
L 0.05 |
H 20 |
|
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 60
Unit: mg/dl
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 12 of 41 |
Chema Diagnostica - Guidelines for automatic analyzers - Olympus AU 400 - 600 - 640
Analyzer: |
Olympus AU 400 - 600 - 640 |
Application: |
BILIRUBIN DIRECT FL - Code DD F125 / F500 CH |
Preparation: |
R1 - INSTALL LIQUID READY TO USE REAGENT A |
|
R2 - INSTALL LIQUID READY TO USE REAGENT B |
Storage: |
REFRIGERATE AT 2-8°C |
Stability: |
AS INDICATED IN THE LABEL |
TEST NAME: |
BILD |
|
|
|
|
|
|
Sample: |
Volume |
10 μl |
Dilution |
0 μl |
Pre-dilution Rate: 1 |
||
Reagents: |
R1 volume |
200 μl |
Dilution |
0 μl |
|
|
|
|
R2 volume |
50 μl |
Dilution |
0 μl |
|
|
|
Wavelenght: |
Pri. 540 |
Sec. 660 |
Min OD |
|
Max OD |
|
|
Method: |
END |
|
|
L |
|
H |
|
Reaction slope: + |
|
|
Reagent OD limit: |
|
|
||
|
|
|
|
First L |
- 0.1 |
First H |
0.5 |
|
|
|
|
Last L |
- 0.1 |
First H |
0.5 |
Measuring point 1: First |
Last |
27 |
|
|
|
|
|
Measuring point 2: First |
Last |
10 |
|
|
|
|
|
Linearity: |
|
|
|
Dynamic range: |
|
|
|
No Lag Time: |
|
|
|
L 0.05 |
H 20 |
|
|
|
|
|
|
Correlation factor: |
|
|
|
|
|
|
|
A 1 |
B 0 |
|
|
On-board stability period: 60
Unit: mg/dl
IUS.422.0.2 |
Directions for use |
Edition 2010-08-01 |
page 13 of 41 |
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