Metrohm 790IC User Manual
CH-9101 Herisau/Switzerland
Phone ++41 71 353 85 85
Fax ++41 71 353 89 01
Internet www.metrohm.ch
E-Mail info@metrohm.ch
790 Personal IC
Program «790 PC Software 1.0»
790 Personal IC
POWER
8.790.1003 Instructions for Use
14.08.2000 / dö
Table of contents
Table of contents
1 Introduction
1.1 Instrument description
1.2 Parts and controls
1.3 Information on the Instructions for Use
1.3.1 Organization ..........................................................................................10
1.3.2 Notation and pictograms ......................................................................11
1.4 Safety notes
1.4.1 Electrical safety .....................................................................................12
1.4.2 General precautionary rules..................................................................12
2 Installation
2.1 Overview
2.1.1 Flow chart..............................................................................................13
2.1.2 Connections in the 790 Personal IC......................................................13
2.2 Setting up the instrument
2.2.1 Packaging .............................................................................................15
2.2.2 Check .................................................................................................... 15
2.2.3 Location................................................................................................. 15
2.3 Attaching the accessories
2.3.1 Connection of detector block................................................................15
2.3.2 Connection of syringe and aspirating tubing........................................16
2.3.3 Connection of the 6.5324.000 Bottle rack (option)...............................16
2.3.4 Connection of PEEK capillaries ............................................................17
2.3.5 Filter unit PEEK......................................................................................18
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1
1
3
10
12
13
13
15
15
2.4 Mains connection
2.4.1 Setting the mains voltage......................................................................19
2.4.2 Fuses..................................................................................................... 20
2.4.3 Mains cable and mains connection...................................................... 20
2.4.4 On/off switching of the instrument........................................................20
2.5 Connection to the PC
2.5.1 Connecting cable..................................................................................21
2.5.2 Software installation ..............................................................................21
2.5.3 Basic settings........................................................................................22
2.6 High-pressure pump
2.6.1 Removing the transport security screws............................................... 25
2.6.2 Installing the pulsation dampener (option) ...........................................25
2.6.3 Connecting the eluent bottle................................................................. 27
2.6.4 Deaerating the pump and rinsing the pulsation dampener..................27
2.7 Precolumns and separating columns
2.7.1 General information on precolumns .....................................................30
2.7.2 Precolumns with cartridge head ........................................................... 30
2.7.3 Precolumn glass cartridges with cartridge holder ................................ 32
2.7.4 IC anion precolumn SUPERSEP...........................................................33
2.7.5 General information on separating columns.........................................34
2.7.6 Selection of the sample loop.................................................................34
2.7.7 Installation of the separating column without suppressor....................35
2.7.8 Installation of the separating column with suppressor .........................36
..................................................................................................
...........................................................................................
............................................................................................
...............................................................
19
21
25
30
790 Personal IC
I
Table of contents
2.8 Suppressor module
2.8.1 General information on suppressor module......................................... 37
2.8.2 Preparation of the peristaltic pump....................................................... 37
2.8.3 Connection of supply bottles................................................................ 40
2.8.4 Connection of the suppressor module.................................................41
2.9 Putting into operation
2.9.1 Putting into operation without suppressor (cation system).................. 43
2.9.2 Putting into operation with suppressor (anion system)........................ 44
2.10 Connection of external devices
2.10.1 Connection of the 750 Autosampler ..................................................... 46
2.10.2 Connection of the 766 IC Sample Processor .......................................47
3 Operating tutorial
3.1 Requirements
3.2 Preparations
3.3 Calibration
3.4 Sample determination
........................................................................................................
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37
43
46
51
51
52
53
60
4 Operation
4.1 Fundamentals of the operation
4.2 Instrument and software settings
4.3 Systems
...............................................................................................................
4.1.1 Starting/closing the program ................................................................ 63
4.1.2 Glossary ................................................................................................ 64
4.1.3 Overview of program windows .............................................................65
4.1.4 Main window elements ......................................................................... 66
4.1.5 Icons of the main window ..................................................................... 66
4.1.6 Overview of file types............................................................................ 67
4.1.7 Context sensitive menus....................................................................... 68
4.1.8 Keyboard and mouse functions ...........................................................68
4.1.9 Help.......................................................................................................69
4.2.1 Fonts ..................................................................................................... 70
4.2.2 Security system..................................................................................... 70
4.2.3 Global settings ...................................................................................... 71
4.2.4 COM port .............................................................................................. 73
..................................................................................................................
4.3.1 System window ..................................................................................... 74
4.3.2 System file handling.............................................................................. 74
4.3.3 System functions................................................................................... 75
Start/stop hardware and record baseline ..................................... 75
Start/stop determinations.............................................................. 75
Print system parameters ............................................................... 76
4.3.4 PC icon.................................................................................................. 77
4.3.5 Watch window.......................................................................................77
..........................................................................
......................................................................
63
63
70
74
II
790 Personal IC
Table of contents
4.3.6 Instrument icon......................................................................................78
Menu options for instrument icon ................................................. 78
System parameters for disconnected system .............................. 78
Instrument control for connected system ..................................... 79
Time program................................................................................80
Configuration................................................................................. 82
Hardware settings ......................................................................... 82
4.3.7 System state window ............................................................................84
Status messages ..........................................................................84
Error messages.............................................................................85
4.4 Methods
4.4.1 Method file handling..............................................................................86
4.4.2 Passport ................................................................................................ 86
4.4.3 Method setup ........................................................................................93
4.4.4 Integration ............................................................................................. 96
4.4.5 Calibration and quantification ............................................................. 100
4.4.6 Report output ......................................................................................112
..................................................................................................................
General..........................................................................................87
Sample .......................................................................................... 88
Column..........................................................................................89
Eluent ............................................................................................90
Comment.......................................................................................90
Method Log...................................................................................91
Data Log........................................................................................ 92
General..........................................................................................93
Processing ....................................................................................93
Math ..............................................................................................94
Setup .............................................................................................97
Events............................................................................................ 98
General information..................................................................... 100
Notations .....................................................................................101
External standard calibration.......................................................102
Component table ........................................................................102
Peak identification .......................................................................104
Concentration table.....................................................................106
Calibration curve .........................................................................108
Update calibration.......................................................................110
Load and save calibration data...................................................111
Import and export calibration data..............................................111
Put out calibration curves............................................................ 111
Report options............................................................................. 112
86
790 Personal IC
4.5 Chromatograms
4.5.1 Chromatogram window....................................................................... 124
4.5.2 Chromatogram file handling ...............................................................125
4.5.3 Graphical representation..................................................................... 128
4.5.4 Peak editor ..........................................................................................133
..................................................................................................
124
Open chromatogram...................................................................125
Save chromatogram.................................................................... 126
Close chromatogram ..................................................................126
Delete chromatogram ................................................................. 126
Export chromatogram .................................................................127
Appearance................................................................................. 128
Other graphical functions............................................................ 132
Switching on/off the peak editor .................................................133
Peak editor functions ..................................................................133
Moving the cursor .......................................................................134
III
Table of contents
4.5.5 Printing ................................................................................................ 135
Page layout for printing...............................................................135
Printer settings ............................................................................ 136
Print preview................................................................................ 136
Printing ........................................................................................ 136
4.5.6 Miscellaneous functions ..................................................................... 136
Reintegration...............................................................................136
Recalibration ............................................................................... 136
Subtraction of a chromatogram..................................................137
Data compression....................................................................... 137
Invert chromatogram................................................................... 137
4.6 Sample queue
4.6.1 Sample queue file handling ................................................................ 138
4.6.2 Sample queue control......................................................................... 139
4.6.3 Sample queue editor...........................................................................141
4.7 Batch reprocessing
4.7.1 Batch reprocessing queue file handling............................................. 144
4.7.2 Perform batch reprocessing ............................................................... 145
4.7.3 Batch reprocessing queue editor ....................................................... 149
......................................................................................................
Open sample queue ................................................................... 138
Save sample queue .................................................................... 138
Delete sample queue.................................................................. 138
Sample queue overview table..................................................... 139
Start sample queue..................................................................... 140
Pause sample queue.................................................................. 141
Cancel last run ............................................................................ 141
Reset sample queue...................................................................141
Open queue editor window ........................................................ 141
Sample queue editor functions................................................... 143
Print sample queue.....................................................................143
Close sample queue editor......................................................... 143
Open batch reprocessing queue................................................ 144
Create new batch reprocessing queue ...................................... 144
Save batch reprocessing queue................................................. 144
Reprocess options window ........................................................ 145
Merge chromatograms ............................................................... 148
Open batch reprocessing queue editor window ........................ 149
Batch reprocessing queue editor functions ............................... 150
Print batch reprocessing queue ................................................. 150
Close batch reprocessing queue editor ..................................... 150
............................................................................................
138
144
IV
790 Personal IC
Table of contents
5 Notes – Maintenance – Faults
5.1 Practical notes on ion chromatography
5.1.1 Separating columns ............................................................................151
5.1.2 High-pressure pump...........................................................................152
5.1.3 Eluents................................................................................................. 153
5.1.4 Peristaltic pump...................................................................................153
5.1.5 Suppressor module.............................................................................154
5.1.6 Connections ........................................................................................154
5.2 Maintenance and servicing
5.2.1 General information............................................................................. 155
5.2.2 Passivation ..........................................................................................155
5.2.3 Recycling.............................................................................................156
5.2.4 Shutdown ............................................................................................156
5.2.5 Changing separating columns............................................................156
5.2.6 Maintenance work at the pump head .................................................158
5.2.7 Regeneration of the suppressor module ............................................162
5.2.8 Cleaning the suppressor.....................................................................163
5.2.9 Replacing the suppressor................................................................... 165
5.2.10 Exchanging the pump tubing.............................................................. 167
5.3 Faults and malfunctions
5.3.1 Error messages...................................................................................168
5.3.2 Malfunctions and their rectification ..................................................... 168
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....................................................................................
...................................................
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151
151
155
168
5.4 Diagnostic tests / Validation / GLP
6 Appendix
6.1 Technical data
6.2 Standard equipment
6.3 Optional accessories
.............................................................................................................
.....................................................................................................
6.1.1 Conductivity measurement ................................................................. 171
6.1.2 Conductivity detector .......................................................................... 171
6.1.3 Injection valve......................................................................................171
6.1.4 High-pressure pump...........................................................................172
6.1.5 Peristaltic pump...................................................................................173
6.1.6 Suppressor module.............................................................................173
6.1.7 RS232 interface ...................................................................................173
6.1.8 Remote interface .................................................................................174
6.1.9 Mains connection................................................................................174
6.1.10 Safety specifications ...........................................................................174
6.1.11 Electromagnetic compatibility (EMC).................................................. 174
6.1.12 Ambient temperature...........................................................................174
6.1.13 Housing ...............................................................................................175
6.3.1 Accessories for 790 Personal IC......................................................... 179
6.3.2 Separating columns and precolumns.................................................181
6.3.3 Additional devices and cables............................................................185
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171
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170
171
176
179
790 Personal IC
6.4 Warranty and conformity
6.4.1 Warranty ..............................................................................................186
6.4.2 EU Declaration of conformity ..............................................................187
6.4.3 Certificate of conformity and system validation..................................188
6.5 Index
......................................................................................................................
...................................................................................
186
189
V
Table of contents
List of figures
Fig. 1: Front of the 790 Personal IC............................................................................3
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
: Rear of the 790 Personal IC ............................................................................4
: Interior of the 2.790.0010 Personal IC.............................................................6
: Interior of the 2.790.0020 Personal IC.............................................................8
: Connecting diagram for 2.790.0010 Personal IC without suppressor.......... 14
: Connecting diagram for 2.790.0020 Personal IC with suppressor...............14
: Connectors for capillaries .............................................................................17
: 6.2821.100 Filter unit PEEK...........................................................................18
: Setting the mains voltage..............................................................................20
: Connection of the pulsation dampener (option) ...........................................26
Fig. 11
: Installation of precolumn cartridges with cartridge head..............................31
Fig. 12
: Installation of precolumn glass cartridges with cartridge holder ..................33
Fig. 13
: Installation of column without suppressor ....................................................36
Fig. 14
: Installing pump tubings.................................................................................38
Fig. 15
: Installation of column with suppressor..........................................................39
Fig. 16
: Connections at suppressor module..............................................................42
Fig. 17
: Components of the pump head..................................................................159
Fig. 18
: Replacement of the piston seal 92
Fig. 19
: Component of inlet valve 93
Fig. 20
: Assembling the suppressor ........................................................................ 165
92 ............................................................. 159
9292
93 and outlet valve 94
9393
94........................................ 161
9494
VI
790 Personal IC
List of numbered parts and controls
1111 Door to interior..................................3,6,8
2222 Mains pilot lamp .....................................3
3333 Feedthrough for syringe tubing..............3
4444 Feedthrough for aspirating tubing....3,6,8
5555 Opening for detector cable ....................4
6666 Rear panel opening................................4
7777 Opening for outlet capillaries .................4
8888 Knurled screw.........................................4
9999 Detachable rear panel............................4
10
10 Transport security screws.......................4
1010
11
11 Mains switch......................................4,20
1111
12
12 Mains connection plug......................4,20
1212
13
13 Fuse holder........................................4,20
1313
14
14 RS232 interface ......................................4
1414
15
15 Connection for detector block................4
1515
16
16 Remote interface ....................................4
1616
17
17 Serial number .........................................4
1717
18
18 Inlet capillary for injector.................6,8,26
1818
19
19 Mounting rail ..............................6,8,36,39
1919
20
20 Column connection
2020
capillary............................6,8,31,33,36,39
21
21 Sample loop ........................................6,8
2121
22
22 Injection valve .......................6,8,26,36,39
2222
23
23 Aspirating tubing .................................6,8
2323
24
24 Coupling ..............................................6,8
2424
25
25 Syringe tubing......................................6,8
2525
26
26 PEEK coupling................................6,8,39
2626
27
27 Connection capillary.......................6,8,26
2727
28
28 Filter unit PEEK................6,8,18,26,38,39
2828
29
29 Connection capillary.......................6,8,26
2929
30
30 Connection capillary............................6,8
3030
31
31 Purge valve .....................................6,8,26
3131
32
32 Aspirating capillary ..............................6,8
3232
33
33 Fastening screws ................................6,8
3333
34
34 Pump head ...................................6,8,159
3434
35
35 Connection capillary............................6,8
3535
36
36 Connection capillary............................6,8
3636
37
37 Inlet capillary for
3737
detector block............................6,8,36,39
38
38 Detector block ...........................6,8,36,39
3838
Table of contents
39
39 Suppressor module .......................... 8,39
3939
40
40 Tubing cartridge........................... 8,38,39
4040
41
41 Contact pressure lever................. 8,38,39
4141
42
42 Holding clamp .................................. 8,39
4242
43
43 Snap-action lever......................... 8,38,39
4343
44
44 Pump drive........................................ 8,39
4444
45
45 Mounting pin..................................... 8,39
4545
46
46 Compression fitting.......... 17,18,31,33,38
4646
47
47 Compression fitting.............................. 17
4747
48
48 Capillary ..................................... 17,18,33
4848
49
49 Connector with filter............................. 18
4949
50
50 Housing for filter unit PEEK.................. 18
5050
51
51 Connector without filter........................ 18
5151
52
52 Pulsation dampener............................. 26
5252
53
53 Connection to injection valve............... 26
5353
54
54 Connection to purge valve................... 26
5454
55
55 Manufit housing ................................... 31
5555
56
56 Steel connector.................................... 31
5656
57
57 PTFE gasket......................................... 31
5757
58
58 2 Steel meshes .................................... 31
5858
59
59 Precolumn cartridge ............................ 31
5959
60
60 Steel spacer ......................................... 31
6060
61
61 4 Steel meshes .................................... 31
6161
62
62 Manufit pressure screw ....................... 31
6262
63
63 Separating column..................... 31,36,39
6363
64
64 End fitting............................................. 33
6464
65
65 Screw cap for precolumn..................... 33
6565
66
66 Screw cap for precolumn..................... 33
6666
67
67 Precolumn cartridge ............................ 33
6767
68
68 Connection piece................................. 33
6868
69
69 Screw cap for column.......................... 33
6969
70
70 Column holder ................................ 36,39
7070
71
71 Aspirating tubing for H
7171
72
72 Aspirating tubing for H
7272
73
73 Coupling ......................................... 38,39
7373
74
74 Pump tubing for H
7474
75
75 Pump tubing for H
7575
76
76 Stopper ................................................ 38
7676
77
77 Coupling ......................................... 38,39
7777
O................ 38,39
2
............ 38,39
2SO4
................... 38,39
2SO4
O ...................... 38,39
2
790 Personal IC
VII
Table of contents
78
78 Suppressor inlet capillary
7878
for eluent..........................................39,42
79
79 Suppressor outlet capillary
7979
for eluent..........................................39,42
80
80 Suppressor inlet capillary
8080
for H
81
81 Suppressor inlet capillary
8181
for H
82
82 Suppressor outlet capillary
8282
for H
83
83 Suppressor outlet capillary
8383
for H
84
84 Screw.................................................. 159
8484
85
85 Zircon piston.......................................159
8585
86
86 Spring retainer....................................159
8686
87
87 Spring .................................................159
8787
88
88 Piston cartridge ..................................159
8888
89
89 Piston guide sleeve............................159
8989
90
90 Sapphire supporting ring ................... 159
9090
91
91 Piston guide sleeve............................159
9191
O........................................ 38,39,42
2
....................................38,39,42
2SO4
O............................................. 39,42
2
.........................................39,42
2SO4
92
92 Piston seal ..........................................159
9292
93
93 Inlet valve .....................................159,161
9393
94
94 Outlet valve ..................................159,161
9494
95
95 Screw holder.......................................159
9595
96
96 Special tool .........................................159
9696
97
97 Special tool .........................................159
9797
98
98 Valve housing .....................................161
9898
99
99 Sealing ring.........................................161
9999
100
100 Sleeve .................................................161
100100
101
101 Sapphire sleeve ..................................161
101101
102
102 Sapphire sphere .................................161
102102
103
103 Ceramic holder ...................................161
103103
104
104 Seal.....................................................161
104104
105
105 Screw nut............................................165
105105
106
106 Connection piece ...............................165
106106
107
107 Suppressor rotor.................................165
107107
108
108 Suppressor holder..............................165
108108
VIII
790 Personal IC
1.1 Instrument description
1 Introduction
1.1 Instrument description
Personal IC 790
The
phic analyses. The two following versions are available:
is a PC-controlled system for ion chromatogra-
2.790.0010 Personal IC without
•
2.790.0020 Personal IC with
•
The extremely compact housing of the 790 Personal IC contains everything needed to carry out ion chromatography at a high quality level:
Injection valve
•
changer such as the Metrohm 766 IC Sample Processor
High-pressure pump
•
pump with a flow range from 0.2 … 2.5 mL/min and a maximum
pressure of 25 MPa (250 bar)
Column chamber
•
not only thermally stable conditions for the separation column but
also shields the system against electromagnetic interference
Columns
•
separation columns for cations or organic acids – the 790 Personal
IC can accommodate them all
Suppressor
•
integrated in the 2.790.0020 Personal IC – pressure-resistant, with
fully automatic regeneration, highest performance and optimal reproducibility
– for individual injections or for use with a sample
– extremely low-pulsation double piston
– the perfect insulation of the housing provides
– whether anion columns with or without suppression,
– the Metrohm Suppressor Module (MSM) is already
suppressor module
suppressor module
790 Personal IC
Peristaltic pump
•
flow rate of 0.5 … 0.6 mL/min for regeneration and rinsing of the
suppressor module built into the 2.790.0020 Personal IC
Detector
•
stability. The detector temperature of 40 °C varies by less than
0.01°C.
All components which come into contact with eluent and sample are
metal-free.
– conductivity detector with outstanding temperature
– integrated two-channel peristaltic pump with a
1
1 Introduction
operation
The
of the 790 Personal IC takes place via a PC connected
to the RS232 interface with the help of the control and evaluation program
«
»
. This PC program can be used to open pre-
790 Personal IC
pared systems for recording and evaluating chromatograms and
change them if need be. Time programs can also be created in which a
large number of instrument functions can be triggered for each program step. It is also possible to control sample changers such as the
750 Autosampler or the 766 IC Sample Processor via the remote interface.
The operating software for the 790 Personal IC meets all the requirements you could place today on a modern integration software: single
or multi-point calibration, internal or external standard, selectable algorithms for non-linear calibration, various integration modes with integration parameters and integration events, different methods for peak recognition, peak editor, free scaling, superimposing several chromatograms, use of sample tables and batch reprocessing; a powerful and
GLP-conform report generator with output interfaces for monitor, printer
and external databases.
790 Personal IC
2
1.2 Parts and controls
1.2 Parts and controls
1
Door to interior
790 Personal IC
POWER
2134
Fig. 1
: Front of the 790 Personal IC
3
Feedthrough for syringe tubing
for connection of the 6.2816.020 syringe
used for aspirating the sample
2
Mains pilot lamp
lit up when instrument switched on
790 Personal IC
4
Feedthrough for aspirating tubing
3
1 Introduction
88
88
1717
55
Type
WARNING - Fire Hazard -
For continued protection replace only
with the same type and rating of fuse
66 77 66 55
Waste B
Waste A
Transport security screws
88
99
88
1010
1616
Remote
Detector Block
RS 232
1515 1414
Fig. 2: Rear of the 790 Personal IC
f = 50-60 Hz
S = 100 VA
Fuse
100-120V:
1,0A(T)
220-240V:
0,5A(T)
Made by Metrohm Herisau Switzerland
SS
1111
1212
1313
790 Personal IC
4
1.2 Parts and controls
55 Opening for detector cable 1212 Mains connection plug
mains connection, see section 2.4
66 Opening for outlet capillaries
for discharge of eluent, regeneration
solution, and rinsing solution from the
inner compartment
77 Rear panel opening
(closed with plastic stopper) for
additional supply and discharge lines to
and from the inner compartment
88 Knurled screw
for fastening the rear panel 99
99 Detachable rear panel
access to upper part of inner
compartment
1010 Transport security screws
to secure the pump head when the
instrument is transported
1111 Mains switch
to switch instrument on and off:
I = ON 0 = OFF
1313 Fuse holder
changing the fuses, see section 2.4
1414 RS232 interface
connection of the PC
1515 Connection for detector block
1616 Remote interface
remote I/O lines for connection of a
sample changer (750, 766)
1717 Serial number
790 Personal IC
5
1 Introduction
3838
3737
3636
3535
3434
3333
3232
3131
11
1818
1919
2020
2121
1919
2222
3030
POWER
2929
2828 2727 2626
Fig. 3: Interior of the 2.790.0010 Personal IC
(with permanently attached accessories and
1.733.0110 Detector block)
2525
2323
2424
790 Personal IC
6
1.2 Parts and controls
11 Door to interior 2828 Filter unit PEEK (6.2821.100)
1818 Inlet capillary for injector
PEEK capillary,
length L = 24 cm
1919 Mounting rail
for 6.2027.0X0 column holder
2020 Column connection capillary
PEEK capillary,
length L = 30 cm
2121 Sample loop 10 µµL
6.1825.230 PEEK sample loop
2222 Injection valve
2323 Aspirating tubing
PTFE tubing for aspirating the sample;
length L = 65 cm
2929 Connection capillary
PEEK capillary,
length L = 13 cm
3030 Connection capillary
PTFE capillary for connection of the
6.2816.020 syringe,
length L = 70 cm
3131 Purge valve
3232 Aspirating capillary
Connection for 6.1834.010 aspirating
tubing
3333 Fastening screws
for pump head 3434
3434 Pump head (6.2824.100)
2424 Coupling (6.2744.120)
for connection of the 6.2816.020 syringe
2525 Syringe tubing
PTFE tubing for connection of the
syringe,
length L = 35 cm
2626 PEEK coupling (6.2744.040)
2727 Connection capillary
PEEK capillary,
length L = 13 cm
3535 Connection capillary
Connection pump head – purge
valve, fixed mounting
3636 Connection capillary
in pump head, fixed mounting
3737 Inlet capillary for detector block
PEEK capillary, fixed mounting
3838 Detector block (1.732.0110)
790 Personal IC
7
1 Introduction
3838
3737
3636
3535
3434
3333
3232
3131
3030
11
1818
1919
2020
2121
3939
1919
2222
4040
POWER
2929
2828 2727 2626
Fig. 4: Interior of the 2.790.0020 Personal IC
(with permanently attached accessories and
1.733.0110 Detector block)
4545 4444
2525
42424343
4141
2323
2424
790 Personal IC
8
1.2 Parts and controls
1
Door to interior
18
Inlet capillary for injector
PEEK capillary,
length L = 24 cm
19
Mounting rail
for 6.2027.0X0 column holder
20
Column connection capillary
PEEK capillary,
length L = 30 cm
21
Sample loop 20
6.1825.210 PEEK sample loop
22
Injection valve
23
Aspirating tubing
PTFE tubing for aspirating the sample;
length L = 65 cm
L
µµµµ
32
Aspirating capillary
Connection for 6.1834.010 aspirating
tubing
33
Fastening screws
for pump head 34
34
Pump head (6.2824.100)
35
Connection capillary
Connection pump head – purge
valve, fixed mounting
36
Connection capillary
in pump head, fixed mounting
37
Inlet capillary for detector block
PEEK capillary, fixed mounting
38
Detector block (1.732.0110)
24
Coupling (6.2744.120)
for connection of the 6.2816.020 syringe
25
Syringe tubing
PTFE tubing for connection of the
syringe,
length L = 35 cm
26
PEEK coupling (6.2744.040)
27
Connection capillary
PEEK capillary,
length L = 13 cm
28
Filter unit PEEK (6.2821.100)
29
Connection capillary
PEEK capillary,
length L = 13 cm
30
Connection capillary
PTFE capillary for connection of the
6.2816.020 syringe,
length L = 70 cm
39
Suppressor module
(inlet and outlet capillaries are not
shown)
40
Tubing cartridge (6.2755.000)
for 6.1826.060 pump tubing
41
Contact pressure lever
for adjusting the contact pressure
42
Holding clamp
for locking the tubing cartridge into
place
43
Snap-action lever
for releasing the tubing cartridge
44
Pump drive
roller head with contact rollers
45
Mounting pin
for attaching the tubing cartridges
31
Purge valve
790 Personal IC
9
1 Introduction
1.3 Information on the Instructions for Use
Please read through these Instructions for Use carefully before you put
the 790 Personal IC into operation. The Instructions for Use contain
information and warnings to which the user must pay attention in order
to assure safe operation of the instrument.
1.3.1 Organization
These
vide a comprehensive overview of the installation, startup procedure,
operation, fault rectification and technical specifications of this instrument. The Instructions for Use are organized as follows:
To find the required information on the instruments, you will find it an
advantage to use either the
back.
8.790.1003 Instructions for Use
Section 1 Introduction
General description of instrument, parts and controls
and safety notes
Section 2 Installation
Installation of accessories, putting instrument into
operation, connection of sample changers
Section 3 Operating tutorial
Introduction to the operation using an example
Section 4 Operation
Detailed description of the operation
Section 5 Notes – Maintenance – Faults
Notes on ion chromatography, maintenance, fault rectification, diagnostic tests, validation
Section 6 Appendix
Technical data, standard equipment, options, warranty,
declarations of conformity, index
Table of contents
for the 790 Personal IC pro-
or the
Index
at the
As a supplement to the Instructions for Use, the
Monograph "Ion chromatography"
an introduction to the theoretical fundamentals and general information
on separating columns and sample pretreatment.
8.732.2013 IC Applications Collection
The
tains all the
You will find detailed information on the separating columns available
from Metrohm and on special IC applications in the relevant "
tion Bulletins"
your Metrohm agency.
790 Personal IC
10
Application Notes
, which are available on request free of charge from
on the subject of ion chromatography.
is also supplied. This provides
8.732.2003 Metrohm
is also supplied; this con-
Applica-
1.3 Information on the Instructions for Use
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these Instructions for Use:
Range
SYSTEM STATE
<OK>
[ Ctrl ]
35
35
3535
12
12
1212
26
26
2626
Menu item, parameter or entry
value
Program window
Button
Key
Part or control of 790
Part or control of 750
Part or control of 766
Hazard
This symbol draws attention to a
possible danger to life or of injury if
the associated directions are not
followed correctly.
790 Personal IC
Warning
This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed correctly.
Caution
This symbol marks important
information. First read the associated directions before you continue.
Comment
This symbol marks additional
information and tips.
11
1 Introduction
1.4 Safety notes
1.4.1 Electrical safety
While electrical safety in the handling of the 790 Personal IC is assured
in the context of the specifications IEC 1010-1 (protection class 1, degree of protection IP20), the following points should be noted:
Mains connection
•
Setting of the mains voltage, checking the mains fuse and the
mains connection must be effected in accordance with the instruc-
tions in section 2.4.
Opening the 790 Personal IC
•
If the 790 Personal IC is connected to the power supply, the instrument must not be opened nor must parts be removed from it, otherwise there is a danger of coming into contact with components which
are live. Hence, always disconnect the instrument from all voltage
sources before you open it and ensure that the mains cable is
disconnected from mains connection 12
12 !
1212
Protection against static charges
•
Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 790 Personal IC, you should earth yourself and any tools
you are using by touching an earthed object (e.g. housing of the
instrument or a radiator) to eliminate any static charges which exist.
1.4.2 General precautionary rules
Handling of solvents
•
Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.
790 Personal IC
12
2.1 Overview
2 Installation
2.1 Overview
2.1.1 Flow chart
The following flow chart provides an overview of all installation work. You
will find more detailed information in the relevant sections.
sect. 2.2
Setting up
Setting up
Setting upSetting up
Installing accessories
Installing accessories
Installing accessoriesInstalling accessories
Mains connection
Mains connection
Mains connectionMains connection
Connecting PC
Connecting PC
Connecting PCConnecting PC
sect. 2.2
sect. 2.2sect. 2.2
sect. 2.3
sect. 2.3
sect. 2.3sect. 2.3
sect. 2.4
sect. 2.4
sect. 2.4sect. 2.4
sect. 2.5
sect. 2.5
sect. 2.5sect. 2.5
Connecting high pr. pump
Connecting high pr. pump
Connecting high pr. pumpConnecting high pr. pump
Precolumn
Precolumn
PrecolumnPrecolumn
No
No
NoNo
Installing sample loop
Installing sample loop
Installing sample loopInstalling sample loop
Connecting column
Connecting column
Connecting columnConnecting column
Suppressor
Suppressor Connecting suppressor module
SuppressorSuppressor
No
No
NoNo
Conditioning
Conditioning
ConditioningConditioning
Connecting sample changer
Connecting sample changer
Connecting sample changerConnecting sample changer
sect 2.6
sect 2.6
sect 2.6sect 2.6
Yes
Yes
YesYes
sect. 2.7.6
sect. 2.7.6
sect. 2.7.6sect. 2.7.6
sect. 2.7.7/8
sect. 2.7.7/8
sect. 2.7.7/8sect. 2.7.7/8
Yes
Yes
YesYes
sect. 2.9
sect. 2.9
sect. 2.9sect. 2.9
sect. 2.10
sect. 2.10
sect. 2.10sect. 2.10
Precolumn with cartridge head
Precolumn with cartridge head
Precolumn with cartridge headPrecolumn with cartridge head
Precolumn with cartridge holder
Precolumn with cartridge holder
Precolumn with cartridge holderPrecolumn with cartridge holder
IC anion precolumn SUPERSEP
IC anion precolumn SUPERSEP
IC anion precolumn SUPERSEPIC anion precolumn SUPERSEP
Connecting suppressor module
Connecting suppressor moduleConnecting suppressor module
sect. 2.7.2
sect. 2.7.2
sect. 2.7.2sect. 2.7.2
sect. 2.7.3
sect. 2.7.3
sect. 2.7.3sect. 2.7.3
sect. 2.7.4
sect. 2.7.4
sect. 2.7.4sect. 2.7.4
sect. 2.8
sect. 2.8
sect. 2.8sect. 2.8
2.1.2 Connections in the 790 Personal IC
The two following illustrations show the internal connections in the 790
Personal IC in schematic form. The meanings of the various numbered
components are given in the detailed illustrations and descriptions in
ections 2.2 – 2.10
s
790 Personal IC
.
13
2 Installation
20
32
32
3232
31
31
3131
34
34
3434
29
29
2929
36
36 35
3636
35
3535
28
28 27
27
2828
2727
18
18 21
21 22
1818
2121
20 60
2020
22
2222
Injection valve
Column
23
23
2323
60 37
6060
37 38
3737
38
3838
Detector
6.1834.010
30
30
3030
6.2821.090
Eluent
Fig. 5
: Connecting diagram for 2.790.0010 Personal IC without suppressor
36
32
32
3232
34
34
3434
36 35
3636
35
3535
28
28 27
27
2828
2727
49
49
4949
24
24
2424
18
18 21
1818
21 22
22
2121
2222
20
20 60
2020
78
78
7878
25
25
2525
Sample
60 75
6060
Column
80
80
8080
75
7575
76
76 26
7676
26 37
2626
39
39
3939
Waste
37 38
3737
Detector
77
77
7777
79
79
7979
38
3838
29
29
2929
28
28
H2O
68
68
6868
2828
72
7272
74
74
7474
31
31
3131
6.1834.010
14
30
30
3030
6.2821.090
Eluent
Fig. 6
: Connecting diagram for 2.790.0020 Personal IC with suppressor
790 Personal IC
49
49
4949
25
25
2525
24
24
2424
Sample
69
69
6969
23
23
2323
H2SO
71
71
7171
70
70 72
7070
4
Waste
2.2 Setting up the instrument
2.2 Setting up the instrument
2.2.1 Packaging
The 790 Personal IC is supplied together with the separately packed
accessories in special packagings containing shock-absorbing foam
linings designed to provide excellent protection. The instrument itself is
packed in an evacuated polyethylene bag to prevent the ingress of
dust. Please store all these special packagings as only they assure
transport of the instrument free from damage.
2.2.2 Check
After receipt, immediately check whether the shipment is complete and
has arrived without damage (compare with delivery note and list of
accessories in
instructions in
2.2.3 Location
section 6.2
section 6.4.1
). In the case of transport damage, see
"Warranty".
Position the instrument in the laboratory at a location convenient for operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals.
To avoid disturbing temperature influences on the insulated column
compartment, the instrument must be protected against direct
sunlight.
2.3 Attaching the accessories
2.3.1 Connection of detector block
The metal-free
delivery of the 790 Personal IC; it must be inserted in the instrument
and connected up. Proceed as follows:
1 Note the cell constant
•
1.732.0110 Detector block
The cell constant
detector block. Note this value; it must subsequently be en-
tered in the software in order to ensure that an exact display
of the conductivity is obtained (see
c = XX,X /cm
belongs to the scope of
is printed on the rear of the
section 2.5.3
).
790 Personal IC
2 Install detector block
•
Unscrew the four knurled screws
of the 790 Personal IC and remove rear panel (see
•
Position detector block
vided in the 790 Personal IC and push fully to the front (see
Fig. 3
and
Fig. 4
).
8888
from the top rear panel
Fig. 2
38
38
from the back in the space pro-
3838
).
9999
15
2 Installation
•
Insert the cable permanently attached to the detector block
38
38
in one of the openings
3838
the openings
•
Replace rear panel
7777
of the rear panel
the four knurled screws
9999
5555
and the outlet capillary in one of
9999
.
and screw to the 790 Personal IC using
8888
.
3 Connect detector block
•
Plug the gray connecting cable permanently attached to the
38
detector block
38
into connection
3838
15
15
"Detector Block" of the
1515
790 Personal IC and fasten to the instrument by tightening
the screws in the cable connector (see
Fig. 2
).
4 Connect waste container
•
Lead the outlet capillary of the detector block
38
38
to a suffi-
3838
ciently large waste container and fix in place.
2.3.2 Connection of syringe and aspirating tubing
22
For manual filling of the sample loop
the 6.2816.020 Syringe, the syringe tubing
23
PTFE aspirating tubing
23
are needed. These accessories are mounted
2323
as follows:
1 Connect syringe to syringe tubing
•
Pull syringe tubing
25
25
2525
hand out of feedthrough
Fig. 4
).
•
Mount a PEEK compression fitting
the end of the syringe tubing
(6.2744.120) to the compression fitting
•
Push 6.2816.020 Syringe (without needle) as far as it will go
into the connection of coupling
2 Install aspirating tubing
•
Pull aspirating tubing
hand out of feedthrough
Fig. 4
).
22
mounted on the injection valve,
2222
25
25
with coupling
2525
connected to injection valve
3333
as far as desired (see
46
46
(see
4646
25
25
and screw the coupling
2525
24
24
.
2424
23
23
connected to injection valve
2323
4444
as far as desired (see
section 2.3.4
46
46
.
4646
24
24
2424
22
22
2222
Fig. 3
Fig. 3
and the
by
and
) to
24
24
2424
22
22
by
2222
and
2.3.3 Connection of the 6.5324.000 Bottle rack (option)
The optional available 6.5324.000 Bottle rack for supply vessels can be
placed on top of the 790 Personal IC. The accessories include the supply vessels for eluent (2 L), regeneration solution (1 L) and rinsing solution (1 L).
790 Personal IC
16
2.3 Attaching the accessories
2.3.4 Connection of PEEK capillaries
For the connections between high-pressure pump and detector block
6.1831.010 PEEK capillaries
which are connected using either
tings (long)
These PEEK connectors can also be used to connect 6.1822.010 PTFE
microcapillaries (i.d. = 0.3 mm). Proceed as follows:
Capillaries fitted with new connectors must have a perfectly flat cut
surface. To cut PEEK or PTFE capillaries it is best to use the
6.2621.080 Capillary tubing cutter
1 Mount compression fitting
Slide a compression fitting
fitting
fastened as shown in
2 Insert capillary in connection
Push capillary end in the corresponding connection as far as it
will go (to avoid dead volume).
3 Tighten compression fitting
Tighten compression fitting
6.2744.070 PEEK compression fittings (short)
or
47
47
(6.2744.070) over the end of the capillary
4747
(i.d. = 0.25 mm, e.d. =
6.2744.010 PEEK compression fit-
.
46
46
(6.2744.010) or a compression
4646
Fig. 7
.
46
46
47
47
or
4646
by hand (never use tools).
4747
1
16
/
) are used
"
48
48
to be
4848
.
46
46 48
4646
Fig. 7
: Connectors for capillaries
48 47
4848
Compression fitting
46
46
4646
(6.2744.010)
Compression fitting
47
47
4747
47
4747
(6.2744.070)
Capillary
48
48
4848
6.1831.010 PEEK capillary or
6.1822.010 PTFE microcapillary
790 Personal IC
17
2 Installation
2.3.5 Filter unit PEEK
6.2821.100 Filter unit PEEK
One
tween the high-pressure pump and the injection valve
Personal IC. This filter unit serves to avoid contamination by abrasive
particles of the piston seals.
The two other filter units PEEK supplied with the 2.790.0020 Personal IC
(with suppressor) are installed between the pump tubings of the peristaltic pump and the inlet capillaries for regeneration and rinsing solution (see
sor module from foreign particles and bacterial growth.
The Filter unit PEEK
tors
For the connection of capillaries
(6.2744.010) or
filter are available as an option with the ordering number 6.2821.110
(set of 10).
For the connection of the filter unit, please note the flow direction
arrow printed on the housing.
section 2.8.2
49
49
(with filter) and
4949
47
47
4747
). These filter units serve to protect the suppres-
28
28
consists of the housing
2828
51
51
(without filter) screwed into the housing
5151
(6.2744.070) must be used. New connectors
28
28
2828
Fig. 8
(see
48
48
PEEK compression fittings
4848
) is already installed be-
22
22
at the 790
2222
50
50
and the two connec-
5050
49
49
4949
50
50
5050
46
46
4646
with
.
48
48 46
4848
Filter unit PEEK (6.2821.100)
28
28
2828
Compression fitting (6.2744.010)
46
46
4646
Capillary
48
48
4848
6.1831.010 PEEK capillary or
6.1822.010 PTFE microcapillary
790 Personal IC
18
46 49
4646
49 50
4949
Fig. 8
: 6.2821.100 Filter unit PEEK
50 51
5050
Connector with filter (6.2821.110)
49
49
4949
Part of 6.2821.100 Filter unit
50
Housing for filter unit
50
5050
Part of 6.2821.100 Filter unit
Connector without filter
51
51
5151
Part of 6.2821.100 Filter unit
51 46
5151
46 48
4646
48
4848
2.4 Mains connection
2.4 Mains connection
Follow the instructions below for connecting to the power supply. If
the instrument is operated with a mains voltage set wrongly and/or
wrong mains fuse, there is a danger of fire!
2.4.1 Setting the mains voltage
Before switching on the 790 Personal IC for the first time, check that the
mains voltage set on the instrument (see
mains voltage. If this is not
on the instrument as follows:
1 Disconnect mains cable
Disconnect mains cable from mains connection plug
790 Personal IC.
the case, you must reset the mains voltage
Fig. 9
) matches the local
12
12
1212
of the
2 Remove fuse holder
13
Using a screwdriver, loosen fuse holder
12
connection plug
3 Check and change fuse if necessary
Carefully take the fuse installed for the desired mains voltage out
of fuse holder
fuse in the fuse holder is marked by the white arrow imprinted
next to the mains voltage range):
100
120 V 1.0 A (slow-blow)
…………
220
240 V 0.5 A (slow-blow)
…………
4 Insert fuse
Change fuse if necessary and reinsert in fuse holder
5 Install fuse holder
Depending on the desired mains voltage, insert fuse holder
in the 790 Personal IC so that the corresponding mains voltage
range can be read normally and the adjacent white arrow points
to the white bar imprinted below the fuse holder (see
12
and take out completely.
1212
13
13
and check its specifications (the position of the
1313
13
below the mains
1313
Metrohm No. U.600.0016
Metrohm No. U.600.0013
13
13
1313
Fig. 9
.
13
13
1313
).
790 Personal IC
19
2 Installation
2.4.2 Fuses
100 – 120 V
Mains switch
11
11
1111
Mains connec-
12
12
1212
11
11
1111
12
12
1212
220 – 240 V
tion plug
----
220
100
120 V
----
240 V
13
13 Fuse holder
1313
13
13
1313
220
240 V
----
----
100
120 V
Fig. 9
: Setting the mains voltage
One of the two fuses 1 A/slow-blow for 100…120 V or 0.5 A/slow-blow
13
for 220…240 V is installed in fuse holder
13
of the 790 Personal IC as
1313
standard.
Ensure that the instrument is never put into operation with fuses of
another type, otherwise there is danger of fire!
For checking or changing fuses, process as described in
2.4.3 Mains cable and mains connection
Mains cable
The instrument is supplied with one of three mains cables
•
6.2122.020 with plug SEV 12 (Switzerland, …)
•
6.2122.040 with plug CEE(7), VII (Germany, …)
•
6.2133.070 with plug NEMA 5-15 (USA, …)
which are three-cored and fitted with a plug with an earthing pin. If a different plug has to be fitted, the yellow/green lead (IEC standard) must
be connected to protective earth (protection class 1).
Any break in the earthing inside or outside the instrument can make it
a hazard!
Mains connection
Plug the mains cable into mains connection plug
sonal IC (see
Fig. 9
).
section 2.4.1
12
12
of the 790 Per-
1212
.
2.4.4 On/off switching of the instrument
The 790 Personal IC is switched on and off using mains switch
When the instrument is switched on, the mains pilot lamp
790 Personal IC
20
2222
lights up.
11
11
1111
.
2.5 Connection to the PC
2.5 Connection to the PC
2.5.1 Connecting cable
Always switch off 790 Personal IC and PC before you connect the two
instruments with the 6.2134.100 Cable.
14
Connect the RS232 interface
rial COM ports at the PC using the 6.2134.100 Cable (9 pin/9 pin). If
only a 25-pin COM interface is available on the PC then the 6.2125.110
Adapter cable or a commercially available adapter must be used.
2.5.2 Software installation
14
at the 790 Personal IC to one of the se-
1414
The PC program
«790 Personal IC 1.0»
is required for the operation
of the 790 Personal IC; this is contained on the 6.6035.003 CD included
in the accessories. This program runs under Windows 95, Windows 98,
Windows NT and Windows 2000 operating systems and is installed as
follows:
1 Install program
•
Insert 6.6035.003 Installation CD into CD drive.
•
Select
installation CD and click on
<Start>
and
Run
. Browse for the
<OK>
setup.exe
file on the
. Follow the instructions
given in the setup program.
The software package will be installed in the desired direc-
tory. Icons are created in the program folder and in the
startup folder. In addition to the program files, the following
folders are installed:
Data
Folder for storage of chromatogram files
Devices
Methods
Reports
Systems
*.chw
(
) and batch reprocessing files (
Folder for storage of device files (
Folder for storage of method files (
Folder for storage of report files (
and graphic files (
*.wmf
)
Folder with subfolders with system files
*.smt
(
) and sample queue files (
*.dev
*.txt
*.que
*.bar
)
*.mtw
)
).
)
)
790 Personal IC
2 Registration
•
Please send us your 8.790.8007 Registration card as soon as
possible. Only registered users will get updated program versions at a special price.
The installed files (incl. system and method files) are generally not
write-protected. To prevent these files from being deleted by mistake,
switch on the write-protection or make a backup copy in another
directory.
21
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