Metrohm 788IC User Manual

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788 IC Filtration Sample Processor

METROHM AG CH-9101 Herisau Switzerland

DES FUSIBLES DE 250V

USE ONLY WITH 250V

100-120V

UNIQUEMENT AVEC

FUSES/EMPLOYER

Metrohm

MetrohmMetrohm

220-240V

Instructions for Use

8.788.1003

CH-9101 Herisau/Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 E-Mail info@metrohm.com Internet www.metrohm.com

788 IC Filtration Sample Processor

Instructions for Use

8.788.1003 03.2001 / up, dö

Table of contents

1 Introduction

1.1 Instrument description ................................................................... 1

1.2 Parts and controls........................................................................... 2

1.3 Information about the Instructions for Use................................. 5

1.3.1 Organization........................................................................... 5

1.3.2 Notation and pictograms ....................................................... 6

1.4 Support dokumentation.................................................................. 7

1.4.1 Application Bulletins............................................................... 7

1.4.2 Application Notes................................................................... 7

1.5 Safety Notes ..................................................................................... 7

1.5.1 Electrical Safety...................................................................... 7

1.5.2 General safety rules ............................................................... 8

2 Installation

2.1 Setting up the instrument ..............................................................9

2.1.1 Packaging ..............................................................................9

2.1.2 Check ..................................................................................... 9

2.1.3 Location.................................................................................. 9

2.1.4 Arrangement of the instruments............................................. 9

.....................................................................................

........................................................................................

2.2 Mains connection ..........................................................................10

2.2.1 Setting the mains voltage..................................................... 10

2.2.2 Fuses.................................................................................... 11

2.2.3 Mains cable and mains connection ..................................... 11

2.2.4 Switching the instrument on/off............................................ 11

2.3 Attaching the accessories ...........................................................12

2.3.1 Connecting the swing head .................................................12

2.3.2 Connecting the keyboard..................................................... 12

2.3.3 Installing the plug cover ....................................................... 12

2.3.4 Installing the splash protection ............................................ 13

2.3.5 Installing the needle or tubing.............................................. 13

2.3.6 Placing the sample rack....................................................... 14

2.3.7 Adjusting the sample rack.................................................... 14

2.3.8 Assembling the ultra-filtration cell ........................................ 16

2.3.9 Tubing connections.............................................................. 19

2.3.10 Tubing connections 788 – 754 Dialysis Unit ...................... 22

2.3.11 Fixing tubing and cables.................................................... 22

788 IC Filtration Sample Processor

Table of contents

2.4 Installation for full control with «IC Net»................................... 23

2.4.1 Connection options.............................................................. 23

2.4.2 General information on interface connections..................... 24

2.4.3 Metrohm IC Systems ........................................................... 25

2.4.4 MIC 1 – Modular anion or cation system

with electronic suppression.................................. 26

2.4.5 MIC 2 Modular anion system

with chemical suppression ................................... 27

2.4.6 MIC 3 Modular anion and cation system

with chemical suppression .................................. 28

2.4.7 MIC 7 Modular anion system

with chemical suppression and dialysis .............. 30

2.5 Installation with compact IC systems........................................ 34

2.5.1 Connection to 761 Compact IC or 790 Personal IC ............ 34

2.6 Installation for simple remote control of the IC system.......... 37

2.6.1 IC system without suppression............................................ 38

2.6.2 IC system with suppression with 788 as "Master"................ 38

2.6.3 IC system with suppression with PC as "Master"................. 39

2.6.4 IC system for simultaneous anion/cation determination...... 39

2.6.5 IC system with sample dialysis............................................ 40

2.7 Connection of devices to the RS232 interface ......................... 41

2.7.1 General information on RS232 interface.............................. 41

2.7.2 Connecting a printer ............................................................ 42

3 Operating tutorial

3.1 Prerequisites / Preparations........................................................ 45

3.2 Configuration................................................................................. 46

3.3 Rack configuration........................................................................ 47

3.4 Methods .......................................................................................... 49

3.5 "Tracing" ......................................................................................... 49

....................................................................

788 IC Filtration Sample Processor

Table of contents

4 Operation

4.1 Fundamentals of operation..........................................................53

4.1.1 Display.................................................................................. 53

4.1.2 Keyboard.............................................................................. 54

4.1.3 Overview of key functions .................................................... 55

4.1.4 Instrument dialog ................................................................. 64

4.1.5 Data entry............................................................................. 66

4.1.6 Text entry.............................................................................. 67

4.2 Basic settings ................................................................................69

4.2.1 Configuration – <CONFIG> key .........................................69

4.2.2 Locking keyboard functions ................................................. 73

4.3 Methoden ........................................................................................ 75

4.3.1 Structure of a method .......................................................... 75

4.3.2 Method parameters – <PARAM> key................................. 76

4.3.3 Programming of sequences................................................. 78

4.3.4 LEARN mode........................................................................ 79

4.3.5 TRACE funktion .................................................................... 79

4.3.6 Commands for sequences................................................... 80

4.3.7 Process control .................................................................... 85

4.3.8 User defined methods.......................................................... 86

4.3.9 POWERUP method .............................................................. 87

.........................................................................................

4.4 Manual operation...........................................................................88

4.4.1 Turning the sample rack / Positioning the samples............. 88

4.4.2 Moving the lift ....................................................................... 88

4.4.3 Setting the sample position.................................................. 89

4.4.4 Pump control........................................................................ 89

4.4.5 Display interface signals ......................................................89

4.4.6 Interface control ................................................................... 90

4.4.7 Print out reports.................................................................... 91

4.5 Sample racks.................................................................................. 93

4.5.1 Standard rack (6.2041.430).................................................. 93

4.5.2 Magnetic code ..................................................................... 93

4.5.3 Rack data ............................................................................. 94

4.5.4 Special beakers.................................................................... 96

4.6 Standard methods......................................................................... 97

4.6.1 Method "PC" and "PC Seg"................................................... 99

4.6.2 Method "SP" and "SP Seg".................................................. 100

4.6.3 Method "761" and "761 Seg"............................................... 101

4.6.4 Method "An Cat" and "AnCatSeg"....................................... 102

4.6.5 Method "Preconc"............................................................... 104

4.6.6 Method "Dialysis"................................................................ 106

4.7 Filtration........................................................................................108

4.6.1 Selecting suitable sample types ........................................ 108

4.6.2 Filtration membrane life...................................................... 108

4.6.3 Selecting a suitable filtration membrane............................ 109

788 IC Filtration Sample Processor

III

Table of contents

5 Interfaces

5.1 Remote interface ......................................................................... 111

5.1.1 Pin assignment of the remote socket .................................111

5.1.2 Functional characteristics ...................................................112

5.2 RS232 interface ........................................................................... 115

5.2.1 General rules for remote control .........................................115

5.2.2 Call-up of objects................................................................116

5.2.3 Trigger.................................................................................117

5.2.4 Status messages ................................................................118

5.2.5 Error messages...................................................................119

5.2.6 Remote control commands ................................................120

5.2.7 Data transmission protocol.................................................127

5.2.8 Handshake..........................................................................128

5.2.9 Pin assignment ...................................................................131

5.2.10 RS232 error rectification....................................................132

6 Appendix

6.1 Error messages ........................................................................... 133

....................................................................................

.......................................................................................

111

133

6.2 Technical data ............................................................................. 135

6.3 Maintenance and servicing........................................................ 137

6.3.1 Maintenance by Metrohm service.......................................137

6.3.2 Care of the unit ...................................................................137

6.3.3 Replacing the pump tubing ................................................138

6.3.4 Replacing the filtration membrane......................................139

6.4 Diagnosis...................................................................................... 141

6.4.1 General Information ............................................................141

6.4.2 Preparing the instrument ....................................................142

6.4.3 Working memory (RAM)......................................................143

6.4.4 Display ................................................................................143

6.4.5 Keyboard ............................................................................144

6.4.6 Remote interface.................................................................145

6.4.7 RS232 interface...................................................................146

6.4.8 External Bus interface .........................................................146

6.4.9 Beeper ................................................................................147

6.4.10 Rack code recognition......................................................147

6.5 Initialize data memory ................................................................ 148

6.6 Validierung / GLP ........................................................................ 150

6.7 Warranty and conformity ........................................................... 151

6.7.1 Warranty..............................................................................151

6.7.2 EU Declaration of conformity..............................................152

6.7.3 Certificate of conformity and system validation..................153

6.8 Standard equipment ................................................................... 154

6.9 Optional accessories.................................................................. 157

6.10 Index............................................................................................ 159

788 IC Filtration Sample Processor

Table of contents

List of figures

Fig. 1

: Side view of the 788 IC Filtration Sample Processor ........................................2

Fig. 2

: Rear of the 788 IC Filtration Sample Processor ................................................4

: Setting the mains voltage ................................................................................ 10

Fig. 3

Fig. 4

: Keyboard connection ......................................................................................12

Fig. 5

: Installing the splash protection........................................................................13

: Needle installation ...........................................................................................14

Fig. 6

Fig. 7

: Sample rack placing........................................................................................14

Fig. 8

: Adjusting the sample rack .............................................................................15

Fig. 9

: Assembling the ultra-filtration cell....................................................................16

Fig. 10

: Installing the pump tubing ...............................................................................19

Fig. 11

: Mount ultra-filtration cell...................................................................................20

Fig. 12

: Opening chain links .........................................................................................22

Fig. 13

: Interconnection with anion or cation IC system

with electronic suppression (MIC 1) ................................................................26

Fig. 14

: Interconnection with anion IC system

with chemical suppression (MIC 2) ................................................................. 27

Fig. 15

: Interconnection with the combined anion and cation IC system

with chemical suppression (MIC 3) ................................................................. 28

Fig. 16

: Interconnection with anion IC system

with chemical suppression and dialysis (MIC 7).............................................30

Fig. 17

: Interconnection of with compact IC systems ..................................................34

Fig. 18

Interconnection with IC system without suppression..........................

Fig. 19:

Fig. 20

Fig. 21

Fig. 22

Fig. 23

Fig. 24

Fig. 25

Interconnection with IC system with suppression with 788 as "Master"

: Interconnection with IC system with suppression with PC as "Master"

Interconnection for anion/cation system.............................................

Interconnection with IC system with dialysis.......................................

: Connection possibilities for the RS232 interface ............................................41

: Schematic representation of the instrument dialog .......................................65

: Installation of the preconcentration column ..................................................104

.......

........

788 IC Filtration Sample Processor

Table of contents

788 IC Filtration Sample Processor

1.1 Instrument description

1 Introduction

1.1 Instrument description

788 IC Filtration Sample Processor

The ion chromatographic determinations including in-line filtration, especially in combination with Metrohm IC system instruments. It is possible to apply and automate existing procedures for sample preparation using the ultra-filtration of the 788 IC Filtration Sample Processor.

The 127 sample tubes with a volume of 11 mL are arranged on the sample rack in three rows, which guarantees easy access and unrestricted programming. Two additional rinsing positions allow sample introduction free from cross-contamination even with widely varying sample matrices. Sample tubes made of polypropylene are standard. To protect the samples from external contamination, the tubes can be hermetically sealed.

can be used for automating

Sample filtration and introduction to the injector is achieved by means of its integrated double-channel peristaltic pump. The sample is conveyed by one channel of the pump through the ultra-filtration cell passing the membrane. At the same time the filtrate is aspirated off from the rear of this membrane and transferred to the sample loop by the second channel of the pump. A PEEK needle for sealed or a PEEK tube for open sample tubes can be used alternatively.

The sequences for the processing of each sample are freely definable within broad boundaries. The same is true for the start and final sequences that are executed once either before or at the end of a sample series. With the help of the LEARN mode, which is provided for creating process methods, methods can be created easily and stored as user methods.

Preprogrammed standard methods for the most common modes of operation allow to use the 788 IC Filtration Sample Processor directly, with only little programming effort. The time frame can be given by the 788 IC Filtration Sample Processor; alternatively, the whole sequence can be managed by the «IC Net» remote control software via the

Queue» (sample table). The RS232 interface built in as standard allows remote control of the 788 IC Filtration Sample Processor from a PC.

788 IC Filtration Sample Processor

1 Introduction

1.2 Parts and controls

4444

3333

2222

1111

Always install splash protection!

55556

1010

1111

1212

1313

1414

1515 16

1616 17

1717 18

1818 19

1919 6666

2020 12

1212 21

2121 22

2222 23

2323 24

2424 25

2525 22

2222

3636

3535

3434

3333

3232

DES FU SI B LE S D E 25 0V

100-120V

USE ONLY WITH 250V

UNIQUEMENT AVEC

FUSES/EMPLOYER

Metrohm

MetrohmMetrohm

Fig. 1

: Side view of the 788 IC Filtration Sample Processor

220-240V

Always install plug cover!

2222

2626

2727

2828

2929

3030

3131

788 IC Filtration Sample Processor

1.2 Parts and controls

Splash protection (6.2751.040)

1111

Must be installed always in operation!

Needle

2222

PEEK needle (6.1835.010) or PEEK tubing (6.1835.020)

Lift

3333

4444

5555 PEEK compression fitting

6666

7777

8888

With swing head attached

Needle guide (4.766.4330)

Attached (part of needle holder (6.2833.000))

(4.766.4320)

PEEK compression fitting (6.2744.010)

PTFE capillaries 1/16 "

Swing head

Attached

PTFE capillary (6.1803.070)

(0.97 mm / 0.40 m) Connection needle – pump tubing

For PEEK needle or tubing

For connection of PEEK or

19 PEEK coupling (6.2744.030)

1919

Connecting compression fitting and pump tubing

20 PEEK capillary (6.1831.060)

2020

For conveying the filtrate into the sample loop

21 PTFE capillary (6.1803.050)

2121

(0.5 mm / 0.2 m) Connecting ultrafiltration cell and filtrate pump tubing

22 PVDF compression fitting

2222

(6.2744.000)

ies (6.1803.0X0) to ultra-filtration cell

23 Ultra-filtration cell (6.2729.110)

2323

(Plexiglass)

24 PTFE capillary (6.1803.060)

2424

(0.97 mm / 0.2 m) Connecting sample pump tubing and ultra-filtration cell

25 PTFE capillary (6.1803.080)

2525

(0.97 mm / 1 m) Connecting ultrafiltration cell and waste

26 Cell holder

2626

For ultra-filtration cell

Connecting PTFE capillar-

(Olive)

(1 m)

Guide chain

9999

For fixing tubings and cables

10 Pump tubing (6.1826.070)

1010

(yellow/yellow) Conveying the sample to the ultra-filtration cell

11 Pump tubing (6.1826.030)

1111

(orange/yellow) Conveying the filtrate to the sample loop

Screw thread for splash protection 30

1212

Mounting pin

1313

For attaching the tubing cartridges

Tubing cartridge (6.2755.000)

1414

For pump tubing (6.1826.0X0)

Pump drive

1515

Roller head with contact rollers

Snap-action lever

1616

For releasing the tubing cartridge

Holding clamp

1717

For locking the tubing cartridge into place

27 Tower

2727

28 PE bottle (6.1608.080)

2828

Connection cable for swing head

2929

Attached, incl. branch plug for remote connection

30 Remote connection

3030

For additional Metrohm instruments

31 Plug cover (6.2752.010)

3131

32 Fuse holder

3232

Changing the fuses, see

33 Mains connection plug

3333

Mains connection, see

34 Mains switch

3434

For switching the instrument on/off: I = ON 0 = OFF

Sample rack (6.2041.430)

3535

(300 mL)

section 2.2.2

section 2.2.3

18 Contact pressure lever

1818

For adjusting the contact pressure

788 IC Filtration Sample Processor

PP sample tube (6.2743.050) (11 mL)

3636

Can be sealed with 6.2743.070 PP caps

1 Introduction

9999

3737

1414

3737

2727

1111

3737

2323

3737

3636

2626

2828

3535

3131

: Rear of the 788 IC Filtration Sample Processor

Fig. 2

Splash protection (6.2751.040)

1111

Must be installed always in operation!

Guide chain

9999

For fixing tubings and cables

Type 1.788.

RS 232 Keyboard

Made by Metro hm Herisau Swi tzerland

35 Sample rack 6.2041.430

3535

36 PP sample tube (6.2743.050) (11 mL)

3636

Can be sealed with 6.2743.070 PP caps

2828

3838

3939

4040

Tubing cartridges (6.2755.000)

1414

For 6.1826.0X0 pump tubing

Tower

2727

PE bottle (6.1608.080)

2828

Plug cover (6.2752.010)

3131

788 IC Filtration Sample Processor

(300 mL)

37 Mounting screws for splash protec-

3737

tion

38 Serial number

3838

39 Keyboard connection

3939

40 RS232 connection

4040

1.3 Information about the Instructions for Use

Please read through these Instructions for Use carefully before operating the 788 IC Filtration Sample Processor. The Instructions for Use contain information and warnings to which the user must pay attention in order to assure safe operation of the instrument..

1.3.1 Organization

These ple Processor provide a comprehensive overview of the installation, startup procedure, operation, fault rectification and technical specifications of this instrument. The Instructions for Use are organized as follows:

8.788.1003 Instructions for Use

Section 1 Introduction

Description of the instrument, parts and controls, safety notes

Section 2 Installation

Mains connection, attachment of accessories, connection to IC systems

Section 3 Operating tutorial

Introduction to the operation

Section 4 Operation

Detailed description of display, keyboard, methods, manual operation, sample racks, standard methods, and filtration

Section 5 Interfaces

Remote interface, RS232 interface and remote control language

for the 788 IC Filtration Sam-

788 IC Filtration Sample Processor

Section 6 Appendix

Error messages, technical data, maintenance and servicing, diagnosis, warranty, declaration of conformity, standard equipment, optional accessories, index

To find the information you require about the instruments please use either the

Quick Reference Guide

daily use as it explains the most important parameters and key functions.

Table of contents

is suitable for use as a reference work for

or the

Index

at the back. The

8.788.1013

1 Introduction

1.3.2 Notation and pictograms

The following notations and pictograms (symbols) are used in these Instructions for Use:

<PUMP>

1515

8989

2626

'Bereich'

******** counter 0/127 PUMP- ready

Switch or key

Part or control of 788

Part or control of 732/733

Part or control of 754

Parameter or entry value

at 788 IC Filtration Sample Processor

Display

Text in keyboard display of 788 IC Filtration Sample Processor

Hazard

This symbol draws attention to a possible danger to life or injury if the associated directions are not followed correctly.

Warning

This symbol draws attention to possible damage to instruments or instrument parts if the associated directions are not followed correctly.

Caution

This symbol marks important information. Read these directions before continuing.

Comment

This symbol marks additional information and tips.

788 IC Filtration Sample Processor

1.4 Support documentation

1.4.1 Application Bulletins

The «Application Bulletin» is a collection of analytical methods, application examples and literature references. All these Application Bulletins are available on request free of charge from your Metrohm supplier.

You will find an updated list of the Application Bulletins at any time under «http://www.metrohm.com».

1.4.2 Application Notes

The «Application Notes» present application information in concentrated form, i.e. on maximum 2 pages. You can order these free of charge from your Metrohm supplier or view them in the Internet under

http://www.metrohm.com» and copy them from there.

1.5 Safety notes

1.5.1 Electrical safety

While electrical safety in the handling of the 788 IC Filtration Sample Processor is assured in the context of the specifications IEC 1010-1 (protection class I), the following points should be noted:

x

Mains connection

Set the mains voltage and check the mains fuse and mains connection in accordance with the instructions in section 2.2.

x

Opening the 788 IC Filtration Sample Processor

To avoid all danger of coming into contact with live components do not open the instrument or remove any parts when the 788 IC Filtration Sample Processor is connected to the power supply. Always disconnect the instrument from all voltage sources before you open it and ensure that the mains cable is disconnected from mains con-

nection 33

33 !

3333

x

788 IC Filtration Sample Processor

Protection against static charges

Electronic components are sensitive to static charging and can be destroyed by discharges. Before you touch any of the components inside the 788 IC Filtration Sample Processor, you should earth yourself and any tools you are using by touching an earthed object (e.g. housing of the instrument or a radiator) to eliminate any static charges which exist.

1 Introduction

1.5.2 General safety rules

x

Install splash protection

To avoid any danger of injury by the needle, the splash protection must always be installed when operating the 788 IC Filtration Sample Processor!

x

Install plug cover

To prevent any contamination of the mains and remote connection by spilled solvents or chemicals, the plug cover must always be installed when operating the 788 IC Filtration Sample Processor!

x

Do not use caps together with the PEEK tube

If you are using the 6.1835.020 PEEK tube, the sample tubes may not be sealed with caps because they cannot be pierced by the PEEK tubing and the tubing may be damaged thereby.

x

Handling of solvents

Check the pump tubing and all input and output leads periodically for possible leaks. Follow the relevant instructions regarding the handling of flammable and/or toxic solvents and their disposal.

x

Regular exchange of pump tubings

Pump tubings constitute consumable material and must be replaced from time to time (see section 6.3.3). Suitable measures must be taken so that any leak which might occur in the pump tubing or connections during unattended operation will cause no damage (collection device for any liquid which may leak out).

788 IC Filtration Sample Processor

2.1 Setting up the instrument

2 Installation

2.1 Setting up the instrument

2.1.1 Packaging

The 788 IC Filtration Sample Processor is supplied together with the separately packed accessories in special packaging containing shockabsorbing foam linings designed to provide excellent protection. The actual instrument is packed in an evacuated polyethylene bag to prevent the ingress of dust. Please store all these special packagings as only they can assure damage-free transport of the instrument.

2.1.2 Check

After receipt, immediately check whether the shipment is complete and undamaged (compare with delivery note and list of accessories in

section 6.8 section 6.7.1

). In the case of transport damage, see instructions in

"Warranty".

2.1.3 Location

Position the instrument in the laboratory at a location convenient for operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals.

Do not operate the 788 IC Filtration Sample Processor without splash protection 1111 and plug cover 31

31 being installed!

3131

2.1.4 Arrangement of the instruments

788 IC Filtration Sample Processor

To make the connection for conveying the sample filtrate to the injection valve at the 733 IC Separation Center (or 761 Compact IC or 790

Personal IC) with the peek capillary the 788 IC Filtration Sample Processor immediately on the left side of the IC system.

Take precautions to ensure that any leaks from pump tubings or connections cannot cause more damage.

(length 1 m) supplied, position

2020

2 Installation

2.2 Mains connection

Follow the instructions below for connecting to the power supply. If the instrument is operated with the mains voltage set wrongly and/or wrong mains fuse there is a danger of fire!

2.2.1 Setting the mains voltage

3434

3333

3232

100 – 120 V

Before switching on the 788 IC Filtration Sample Processor for the first time, check that the mains voltage set on the instrument (see matches the local mains voltage. If not instrument as follows:

1 Disconnect mains cable

220 -

- 240 V

- 120 V

100 -

220 – 240 V

Fuse holder

100 -

- 120 V

- 240 V

220 -

323232

33 Mains connection

3333

plug

34 Mains switch

3434

: Setting the mains voltage

Fig. 3

, reset the mains voltage on the

Disconnect mains cable from mains connection plug 788 IC Filtration Sample Processor.

of the

3333

Fig. 3

)

2 Remove fuse holder

Using a screwdriver, loosen fuse holder

beside the mains

3232

connection and take out completely.

3 Check fuse

Carefully take the fuse installed for the desired mains voltage out of fuse holder and check its specifications (the position of the fuse in the fuse holder is marked by the white arrow imprinted next to the mains voltage range):

100}}}}120 V 0.5 A (slow-blow)

220}}}}240 V 0.25 A (slow-blow)

788 IC Filtration Sample Processor

Metrohm-Nr. U.600.0013

Metrohm-Nr. U.600.0010

2.2 Mains connection

4 Insert fuse

Change fuse if necessary and reinsert in fuse holder

3232

5 Install fuse holder

Depending on the desired mains voltage, insert fuse holder

3232

in the 788 IC Filtration Sample Processor so that the corresponding mains voltage range can be read normally and the adjacent white arrow points to the white bar imprinted on the right side of the fuse holder (see

Fig. 3

2.2.2 Fuses

One of the two fuses 0.5 A/slow-blow for 100}120 V or 0.25 A/slow-

blow for 220}240 V is installed in fuse holder Sample Processor as standard.

of the 788 IC Filtration

3232

Ensure that the instrument is never put into operation with fuses of another type, otherwise there is danger of fire!

For checking or changing fuses, proceed as described in

2.2.3 Mains cable and mains connection

Mains cable

The instrument is supplied with one of three mains cables

x

6.2122.020 with plug SEV 12 (Switzerland, })

x

6.2122.040 with plug CEE(7), VII (Germany, })

x

6.2133.070 with plug NEMA 5-15 (USA, })

which are three-cored and fitted with a plug with an earthing pin. If a different plug has to be fitted, the yellow/green lead (IEC standard) must be connected to protective earth (protection class I).

Any break in the earthing inside or outside the instrument can make it a hazard!

Mains connection

Plug the mains cable into mains connection plug Filtration Sample Processor (see

Fig. 3

section 2.2.1

of the 788 IC

3333

2.2.4 Switching the instrument on/off

The 788 IC Filtration Sample Processor is switched on and off using

mains switch

788 IC Filtration Sample Processor

3434

2 Installation

2.3 Attaching the accessories

For attaching the accessories at the 788 IC Filtration Sample Processor, proceed in the order described below.

2.3.1 Connecting the swing head

Plug in the branch plug of the connection cable attached to the swing head into the remote connection socket at the 788 IC Filtration Sample Processor and screw it onto this connection using a screw-driver (see

2.3.2 Connecting the keyboard

Fig. 1

permanently

29 29

Connecting the keyboard

Connect the 6.2142.010 keyboard to the keyboard connection "Keyboard". For disconnection, press the plug together slightly on both sides.

Switch on instrument

Switch on the 788 IC Filtration Sample Processor with mains

switch lights up. The instrument is initialized and the lift is raised completely.

. The keyboard display

3434

2.3.3 Installing the plug cover

3939

Fig. 4

: Keyboard connection

To prevent any contamination of the mains and remote connection by spilled solvents or chemicals, the 6.2752.010 plug cover must always be installed when operating the 788 IC Filtration Sample Processor!

Install the plug cover mains connection plug

Fig. 2

788 IC Filtration Sample Processor

in the corresponding guide groove above

3131

and remote connection

3333

3030

(see

Fig. 1

and

2.3 Attaching the accessories

2.3.4 Installing the splash protection

To avoid any danger of injury by the needle, the 6.2751.040 splash protection must always be installed when operating the 788 IC Filtration Sample Processor!

1 Remove holding screws

Remove the holding screws

screw threads

2 Remove protective film from splash protection

Remove the plastic film glued on both sides of the splash protection

3 Install splash protection

Attach splash protection onto the screw threads at the tower and fix it with the holding screws and the washer using the

6.2621.100 Allen key.

at tower

1212

1111

and the washer mounted on the

3737

using the 6.2621.100 Allen key.

2727

1111 Splash protection

(6.2751.040)

Holding screws

373737

2.3.5 Installing the needle or tubing

1 Remove PEEK compression fitting 5

Remove PEEK compression fitting holder

2 Insert needle

Insert needle tubing) completely into the opening of the needle holder

4444

2222

(6.1835.010) PEEK needle or 6.1835.020 PEEK

: Installing the splash protection

Fig. 5

5555

screwed onto the needle

3737

1111

3737

4444

788 IC Filtration Sample Processor

3 Fix needle or tubing

Tighten compression fitting

use tools!).

(never

5555

in needle holder

4444

by hand

2 Installation

2222 PEEK needle (6.1835.010) or

PEEK tubing (6.1835.020)

4444 Needle holder

5555 PEEK compression fitting

(4.766.4320)

6666 PEEK compression fitting

(6.2744.010)

7777 Swing head

8888 PTFE capillary (6.1803.070)

If you are using the 6.1835.020 PEEK tubing, the sample tubes may not be sealed with caps because they cannot be pierced by the PEEK tubing and the needle may be damaged thereby.

2.3.6 Placing the sample rack

8888

7777

6666

5555

4444

2222

Fig. 6

: Needle installation

Place sample rack

Place sample rack turntable of the 788 IC Filtration Sample Processor according

Fig. 7

Read magnetic code

Press <RESET> to move the rack to the home position, in which the magnetic code can be read (details see

section 4.5

2.3.7 Adjusting the sample rack

If a new sample rack is placed on the 788 IC Filtration Sample Processor for the first time, it must be adjusted on a rack position in the middle row (example: position 37) as follows:

on the

3535

Fig. 7

: Sample rack placing

1 Place sample rack

Place empty sample rack on the 788 IC Filtration Sample Processor and press <RESET> (see

788 IC Filtration Sample Processor

section 2.3.6

2.3 Attaching the accessories

2222 PEEK needle (6.1835.010) or

2222

PEEK tubing (6.1835.020)

Sample rack (6.2041.430)

353535

Adjusting position 37

414141

Adjusting screw

424242

: Adjusting the sample rack

Fig. 8

2 Move to adjusting position

Press <MOVE>, enter number '37' and confirm with <ENTER>. Sample rack and swing head are turned until needle is above the adjusting position 37 (first opening of the middle row).

3 Check needle position

x

Press < of the sample rack.

x

Check needle position: If the needle cannot be lowered unhindered through the upper hole of the adjusting position, continue directly with point 4.

x

Continue lowering the needle by pressing < needle is ca. 1 cm above the lower level of the sample rack.

x

Check needle position: If the needle cannot be lowered unhindered through the lower hole of the adjusting position, continue directly with point 4.

x

Lower needle completely by pressing <

x

Check needle position: If the needle is in the middle of the lower hole, the sample rack must not be adjusted (continue in this case with point 5).

> until the needle is ca. 1 cm above the upper level

> until the

4141

4242

3535

788 IC Filtration Sample Processor

4 Adjust sample rack

4141

on the lower level of the

4242

x

Loosen the three adjusting screws sample rack using the 6.2621.100 Allen key.

x

Carefully turn the two upper levels of the sample rack by hand until the lowered needle is exactly in the middle of the lower hole at the adjusting position

x

Tighten the adjusting screws.

5 Move to rest position

Press <RESET> to move the sample rack to the rest position.

2 Installation

2.3.8 Assembling the ultra-filtration cell

In this section, the assembling of the ultra-filtration cell including the filtration membrane is described. Please consider section 4.7 for evaluating the membrane type and time of exchange. The exchange of filtration membranes is also described in section 6.3.4.

23 43

2323

43 44

4343

4444

45 46

4545

4646

4747

Fig. 9

Ultra-filtration cell (6.2729.110)

2323

Top part of the ultra-filtration cell 47

4343

44 Sealing ring (E.301.0111) 48

4444

Bottom part of the ultra-

4545

filtration cell

Filtration membrane (6.2714.020)

5050

1 Prepare ultra-filtration cell

x

Extract ultra-filtration cell the four dummy stoppers.

x

Using the 6.2621.070 Allen key, completely loosen the 5 screws remove sealing ring

x

Thoroughly rinse the sealing ring, bottom part and top part of the dialysis cell with ultrapure water.

: Assembling the ultra-filtration cell

, separate top part

4646

5050

46 Screws V.022.6030 incl.

4646

47 Outlet for sample filtrate

4747

Inlet for sample solution

4848

49 Outlet for sample solution

4949

4444

3434

48 49

4848

washer 4.754.4090

from its packaging and remove

from bottom part

4343

4950

4949

4545

and

788 IC Filtration Sample Processor

2.3 Attaching the accessories

Use only ultrapure water or ethanol to clean the dialysis cell; organic solvents (e.g. acetone) will cause damage to the plexiglas cell!

2 Prepare filtration membrane

x

Use the 6.2831.010 tweezers to extract a new filtration

membrane

(6.2714.020) from its packaging and immerse

5050

in a Petri dish containing ultra pure water for approx. 2 min until the membrane is completely saturated with water.

Because the filtration membrane it is stretched out thereby, it has to be watered before inserting into the ultra-filtration cell. Otherwise, the membrane could be crumbled in the cell, which leads to blocking.

3 Insert filtration membrane

x

Lay top part

on a paper wipe with its inside facing

4343

upwards.

x

Insert sealing ring

x

Using the tweezers, place the wet dialysis membrane inside

in the appropriate recess in the top part.

4444

the sealing ring on the top part of the filtration cell.

Do not connect the dialysis cell with anything other than the

6.2744.000 PVDF compression fittings supplied. If 6.2744.010 PEEK compression fittings are used, stress cracks may appear in the ultra-filtration cell !

4 Close ultra-filtration cell

46 46

4343

right

x

Insert sealing ring in the appropriate recess in top part

x

Place top part on bottom part

x

Using the 6.2621.070 Allen key, screw the 5 screws

so that both parts lie flush.

4545

in and tighten up.

5 Attach ultra-filtration cell

x

Insert the assembled ultra-filtration cell in the cell holder

26 26

according to Fig. 1 or Fig. 11. The heads of the five screws

fit to the small holes of the cell holder.

46 46

788 IC Filtration Sample Processor

2 Installation

6 Rinse ultra-filtration cell

x

Before rinsing the cell, please finish all tubing connections as described in the following sections.

x

Every time the filtration membrane is changed, the remaining air in the cell and tubings has to be removed. This can be done by rinsing with ultrapure water.

x

A completely installed 788 IC Filtration Sample Processor with ultra-filtration cell is required for processing the following steps. The rinsing solution (e.g. ultrapure water) is stored in a 300 mL PE bottle on the special beaker position 1. The 788 IC Filtration Sample Processor is online and in normal state. The

').

PUMP–'

) and the 788 is ready

pump is switched of (display: ' for use (display: '

x

Turn the sample rack to position the beaker with the rinsing

ready

solution under the needle by pressing <MOVE> followed by choosing 's

pec.1

' with <SELECT>.

x

Lower the lift into rinse position with <LIFT> and <SELECT>

rinse

x

Turn on the pump by pressing <PUMP>. All connected

tubings and the ultra-filtration cell will be rinsed.

x

After adequate time (e.g. 5 min.), switch off the Pump (<PUMP>) and raise the lift to its rest position by pressing <HOME>.

788 IC Filtration Sample Processor

2.3 Attaching the accessories

2.3.9 Tubing connections

The tubing connections on the 788 IC Filtration Sample Processor and the connection to the injection valve of the 733 IC Separation Center must be made as follows:

Pump tubings are consumable material with a lifetime which depends on the contact pressure (see chapter 6.3.3). This is why the tubing cartridges should be raised completely by loosening snap-action lever

on the right-hand side if the pump is to remain switched off for a

1616

considerable length of time (the set contact pressure remains unchanged).

The 6.1826.0X0 pump tubing is made of PVC and must not be used for rinsing with solutions which contain acetone. In such cases, rinse with different pump tubing or a different pump.

8/21

8/218/21

19 10/11

1919

10/11 43

10/1110/11

43 14

4343

14 18

1414

18 43

1818

43 19

16 66666666

4343

1616

1919

24/20

24/2024/20

Abb. 10

Line 1: Conveying the sample

to the ultra-filtration cell

8888 PTFE capillary (6.1803.070)

6666 PEEK compression fitting

(6.2744.010)

PEEK coupling (6.2744.030)

1919

10 Pump tubing (6.1826.070)

1010

43 Stopper (yellow/yellow)

4343

Tubing cartridge (6.2755.000)

1414

Contact pressure lever

1818

16 Snap-action lever

1616

PTFE capillary (6.1803.060)

2424

: Installing the pump tubing

Line 2: Conveying the filtrate

to the injection loop

21 PTFE capillary (6.1803.050)

2121

6666 PEEK compression fitting (6.2744.010)

19 PEEK coupling (6.2744.030)

1919

11 Pump tubing 6.1826.030

1111

43 Stopper (orange/yellow)

4343

14 Tubing cartridge (6.2755.000)

1414

18 Contact pressure lever

1818

16 Snap-action lever

1616

20 PEEK capillary (6.1831.060)

2020

788 IC Filtration Sample Processor

2 Installation

1 Install pump tubing

x

Release tubing cartridge sing down snap-action lever

on the 788 IC Filtration Sample Processor (see

1313

x

Press contact pressure lever as far as it will go.

x

Insert pump tubings shown in the corresponding holder on the left-hand side of each tubing cartridge.

x

Place both tubing cartridges on mounting pin down on the right-hand side until snap-action lever into position on holding clamp formed in the pump tubing.

2 Connection needle – pump tubing 10

x

Mount a PEEK compression fitting PTFE capillary

x

Screw the PTFE capillary on to the PEEK compression fitting needle holder

x

Insert PTFE capillary into the guide chain

section 2.3.10

x

Screw a coupling the other end of the PTFE capillary.

x

Push coupling on to the inlet end of the pump tubing (yellow/yellow, see

Fig. 10

4444

. The black-black stopper

8888

(see

1919

from holding clamp

1414

and remove from mounting

1616

on the tubing cartridge down

1818

1010

Fig. 6

on to the PEEK compression fitting

Fig. 10

and

in the tubing cartridges

1111

. Take care that no kinks are

1717

6666

on both ends of the

8888

with the PEEK compression fitting

5555

already mounted on

9999

by pres-

1717

must click into

4343

and press

1313

1616

Fig. 1

(see

1010

Fig. 1

1313

click

and

6666

6666 PEEK compression fitting

(6.2744.010)

10 Pump tubing (6.1826.070)

1010

Pump tubing 6.1826.030

1111

19 PEEK coupling (6.2744.030)

1919

21 PTFE capillary (6.1803.050)

2121

PVDF compression fitting

2222

(6.2744.000)

24 PTFE capillary (6.1803.060)

2424

PTFE capillary (6.1803.080)

2525

Cell holder

2626

Top part of the ultra-filtration cell

4343

45 Bottom part of the ultra-filtration cell

4545

Fig. 11

1111

1010

2121 19

1919 6666 22

2222 43

4343 45

4545 24

2424 22

2222

2525

2626

: Mount ultra-filtration cell

788 IC Filtration Sample Processor

2.3 Attaching the accessories

Do not connect the dialysis cell with anything other than the

6.2744.000 PVDF compression fittings supplied. If 6.2744.010 PEEK compression fittings are used, stress cracks may appear in the ultra-filtration cell !

3 Connection pump tubing 10 – ultra-filtration cell

x

Mount a PEEK compression fitting

capillary

x

Mount a PVDF compression fitting PTFE capillary

x

Screw this PEEK capillary with the PVDF compression fitting to inlet opening

x

Push coupling (yellow/yellow) (see

and screw it on to coupling

24 24

2424

of the bottom part of the cell (see

4848

on to the outlet end of the pump tubing

1919

Fig. 10

6666

on the end of the PTFE

1919

on the other end of the

2222

Fig. 9

1010

4 Connection ultra-filtration cell – waste

x

Mount a PVDF compression fitting

capillary

x

Screw this end of the PTFE capillary with the PVDF

2525

compression fitting to outlet opening the cell (see

x

Mount the other end of the PTFE capillary to a waste bottle

Fig. 9

on the end of the PTFE

2222

of the bottom part of

4949

(e.g. optional: 6.1608.070 bottle with 6.1602.150 siphon GL45).

5 Connection ultra-filtration cell – pump tubing 11

x

Mount a PEEK compression fitting

capillary

x

Mount a PVDF compression fitting PTFE capillary

x

Screw this PTFE capillary with the PVDF compression fitting

and screw it on to coupling

2121

to outlet opening for the filtrate

Fig. 9

(see

x

Push coupling (orange/yellow) (see

on to the inlet end of the pump tubing

1919

Fig. 10

6666

on the end of the PTFE

1919

on the other end of the

2222

of the top part of the cell

4747

1111

6 Connection pump tubing 11 – injection valve

(Underlined numbers are parts or controls from the Instructions for Use for the 733 IC Separation Center)

(see

6666

on the end of the PEEK

1919

2828

of the

Fig. 14

2222

and

Fig. 15

x

Mount a PEEK compression fitting

capillary

x

Push coupling (orange/yellow) (see

x

At the 733 IC Separation Center, loosen the rotary nipple

and screw it on to coupling

2020

on to the outlet end of the pump tubing

1919

Fig. 10

screwed onto the interior side of connection

x

Take PTFE suction tubing

8484

732/733 Instructions for Use) completely out of connection

and unscrew from connection "1" of injection valve

2828

6666

1111

2222

788 IC Filtration Sample Processor

2 Installation

x

Pull the PEEK capillary the 733 IC Separation Center and screw onto connection "1" of injection valve

6666

fitting

x

Retighten rotary nipple on the interior side of connection or

7 Tubing connection injection valve – waste

x

Insert 6.2744.020 coupling (from 733 accessories) into connection

x

Screw PTFE suction tubing attached to connection container.

In the case of the 733.0020 IC Separation Center with two injection valves, it is possible to fill both sample loops from the same 788 IC Filtration Sample Processor. For this, connection "1" of valve A (outlet of the sample loop) must be connected to connection "2" of valve B (inlet of the sample loop) using a 6.1803.040 PEEK capillary (15 cm).

to fix the capillary

2828

2121

through the opening

2020

using a 6.2744.010 PEEK compression

6666

2020

of the 733 IC Separation Center.

2727

onto the 6.2744.020 coupling

8484

and lead it into the waste

2727

2121

2222

2828

2222

2.3.10 Tubing connections 788 – 754 Dialysis Unit

If the 788 IC Filtration Sample Processor is used for an IC system with sample dialysis (see at the 754 Dialysis Unit is used for sample conveying instead of the pump at the 788 IC Filtration Sample Processor. Additionally, the ultrafiltration cell is bridged. The tubing connections between the 754 Dialysis Unit and the 733 IC Separation Center have to be made as shown in the inlet of the sample, which is installed as follows:

Install a PEEK compression fitting 6.2744.010 on the inlet end of the PTFE tubing connected to the 754 Dialysis Unit and screw it on the PEEK compression fitting

Fig. 8

2626

section 2.4.7 or section 2.5.8

of the

754 Instruction for Use

(6.1803.030, see

5555

(see

Fig. 6

Fig. 8 of the 754 Instruction for Use

. The only change concerns

2.3.11 Fixing tubing and cables

In order to fix tubing or cables in the guide chain opened with a screw driver or another appropriate tool..

9999

any chain link may be

), the peristaltic pump

)

Fig. 12

788 IC Filtration Sample Processor

: Opening chain links

2.4 Installation for full control with «IC Net»

2.4.1 Connection options

The 788 IC Filtration Sample Processor can be connected to Metrohm IC Systems according to the following techniques:

1. The IC System is totally controlled by the PC software

Data acquisition and direct control of the IC components is done by the PC software «IC Net 2.1» in combination with the 762 IC Interface. The 788 IC Filtration Sample Processor is connected to the IC system both by the RS232 Interface and by the remote interface. Due to direct control of all procedures by the PC software, internal methods of the 788 IC Filtration Sample Processor are not used. This section describes several example combinations of the 788 IC Filtration Sample Processor with Metrohm IC systems based on this technique.

2. Compact IC Systems are remote controlled by PC software

Compact designed IC-Systems 761 Compact IC and 790 Personal IC are also controlled by the PC. The 788 IC Filtration Sample Processor is connected to these IC Systems by remote connection only. Therefore, internal methods of the sample processor are used. Please find a description of the connection of the 788 IC Filtration Sample Processor to the IC Systems 761 Compact IC and 790 Personal IC in The required 788 standard method is given in section 4.6.3.

3. The IC System is connected to PC for simple control

Instead of using the 762 IC Interface, modular Metrohm IC Systems can be simply controlled via the 714 PC Board or 771 IC Compact Interface. These devices just convert the analog signals from the 732 IC Detector to digital data for the PC. For simple control of the IC system, the 732 IC detector has to be connected to the PC additionally via its RS232 port. «IC Net» and the prior software «IC Metrodata» use this serial connection for a simple control of the IC system. As described for compact IC systems the 788 IC Filtration Sample Processor is connected to these IC Systems by remote connection. Also, internal methods of the Sample Processor are used. Please find a description of the connection of the 788 IC Filtration Sample Processor to such IC Systems in methods is given in

section 2.6

section 4.6

. The use of the corresponding 788 standard

section 2.5

788 IC Filtration Sample Processor

2 Installation

2.4.2 General information on interface connections

Before an external device is connected to remote connection 30 RS232 interface 40 always be switched off using mains switch 34

40, the 788 IC Filtration Sample Processor must

4040

34!

3434

30 or

3030

Remote connection

The branch plug of cable plugged into the 25 pin remote interface (see

leading from the swing head (see

2929

section 2.3.1

external devices can be connected to remote connection

Fig. 1

). Any

of this

3030

branch plug. The 788 IC Filtration Sample Processor can be remote controlled via the 8 input lines, the 14 output lines can be used to control external devices.

The pin assignment of the remote interface, its functions, the electrical requirements and conditions are described in

RS232 interface

section 5.1

Many different instruments may be connected via the serial RS232

interface that support the Metrohm remote control language (see

. In addition to Metrohm instruments (e.g. 762 IC Interface)

4040

section 5.2

any printer with serial interface (or parallel interface and parallel/serial converter) or a personal computer (PC) may be connected (see

section 2.7

). Other measuring instrument may be controlled via RS232

interface, as long as it supports serial data transmission.

) is

)

In order to guarantee safe data transmission, it is important to set the same RS232 interface parameters correctly for both instruments connected (see

section 4.2.1

Connection cables

Connecting peripheral instruments to the 788 IC Filtration Sample Processor requires Metrohm cables. Otherwise a safe data transmission may not be guaranteed.

Metrohm cables are labeled with the type of the instrument, which they may be connected with and optionally with the particular socket. Mind the cable ends.

Example

732/1 Remote

cable 6.2125.060

788 IC Filtration Sample Processor

2.4 Installation for full control with «IC Net»

2.4.3 Metrohm IC systems

Metrohm IC systems can be classified into modular IC systems (" and compact IC systems (e.g.

761 Compact IC

790 Personal IC

MIC

The following table gives a review over pre-configured Metrohm IC systems:

Name Application

MIC 1 Anion or cation system with electronic suppression

MIC 2 Anion system with chemical suppression

MIC 3 Anion and cation system with chemical suppression

MIC 4 Anion or cation system with electronic suppression and preconcentration

MIC 5 Anion system with chemical suppression and preconcentration

MIC 6 Anion system with chemical suppression, preconcentration and matrix elimination

MIC 7 Anion system with chemical suppression and dialysis

The following sections describe the electronic connection of some of these systems with the 788 IC Filtration Sample Processor on the base of full control by « 4 and MIC 2 / MIC 5, only the common used systems are described. Additionally, the anion and cation system as the dialysis system

IC Net 2.1

MIC 7

». Due to similar compilation of MIC 1 / MIC

MIC 1

MIC 3

und

MIC 2

as well

are included. Please note that with the MIC 7 system the 788 IC Filtration Sample Processor can be used for dialysis in combination with the 754 Dialysis Unit. For this purpose, the ultra-filtration cell of the 788 IC Filtration Sample Processor should be bridged (see

section 2.3.10

") ).

For connecting the 788 IC Filtration Sample Processor to

and

790 Personal IC

, the remote connection is used only.

761 Compact

Therefore, internal methods of the sample processor are used. Please find the corresponding descriptions in

section 4.6.3

(methods).

section 2.5

(connection) and

788 IC Filtration Sample Processor

2 Installation

2.4.4 MIC 1

Modular anion or cation system with electronic suppression

The 788 IC Filtration Sample Processor is connected to an IC system with electronic suppression consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump and 762 IC Interface as shown in

Fig. 13

6.2134.080 RS232 cable.

6.2128.130

using the 6.2141.110 remote connection cable and the

732

733

6.2115.070

6.2134.100

709762

6.2125.090

6.2141.110

788

6.2134.090

Fig. 13

: Interconnection with anion or cation IC system

6.2134.080

with electronic suppression (MIC 1)

Settings in the «IC Net 2.1» program

Creation of a time program for the 788 IC Filtration Sample Processor:

001 Ctrl INIT 732 002 Move sample 003 Lift work 004 Ctrl ZERO 1 005 Ctrl FILL A 1 006 Pump 240 s 007 Ctrl INJECT A 1

 Initialize remote interface at 732  Move needle to sample  Place lift with needle to working position  Trigger autozero at 732  Switch injection valve A at 733 to "Fill"  Fill sample loop with sample during 240 s  Switch injection valve A at 733 to "Inject"

788 IC Filtration Sample Processor

2.4 Installation for full control with «IC Net»

2.4.5 MIC 2

Modular anion system with chemical suppression

The 788 IC Filtration Sample Processor is connected to an anion IC system with chemical suppression consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump, 752 IC Pump Unit, and 762 IC Interface as shown in cable and the 6.2134.080 RS232 cable.

6.2128.130

6.2134.090

6.2115.070

Fig. 14

732

733

using the 6.2141.110 remote connection

6.2125.090

6.2141.110

709

6.2134.100

762

752

788

6.2128.180

6.2134.080

Fig. 14: Interconnection with anion IC system with chemical

suppression (MIC 2)

Settings in the «IC Net 2.1» program

Creation of a time program for the 788 IC Filtration Sample Processor:

001 Ctrl INIT 732 002 Move sample 003 Lift work 004 Ctrl ZERO 1 005 Ctrl FILL A 1 006 Pump 240 s 007 Ctrl INJECT A 1

788 IC Filtration Sample Processor

2 Installation

2.4.6 MIC 3

Modular anion and cation system with chemical suppression

6.2128.130

732

6.2134.100

762

709

The modular anion and cation system can be operated with chemical or electronic suppression. The 788 IC Filtration Sample Processor is connected to the MIC 3 system consisting of two 732 IC Detectors, 733 IC Separation Center (two injectors), two 709 IC Pumps, 753 IC Suppressor Module (if suppression is used), and the 762 IC Interface as shown in

Fig. 15

using the 6.2125.120 remote adapter, the 6.2125.090

remote connection cable and the 6.2134.080 RS232 cable.

6.2125.120

6.2125.090

733

6.2125.090

6.2115.070

732

709

753

6.2143.210

6.2141.110

788

6.2134.090

6.2128.180

6.2134.080

Fig. 15: Interconnection with the combined anion and cation IC

system with chemical suppression (MIC 3)

788 IC Filtration Sample Processor

2.4 Installation for full control with «IC Net»

Settings in the «IC Net 2.1» program

1. Definition of new program parameters for the 788 IC Filtration

Sample Processor under

INJECT A 2 = ---***1*000*** ZERO 2 = ---***0*011***

FILL A 2 = ---***0*010***

2. Program for the 788 IC Filtration Sample Processor:

001 Ctrl INIT 002 Move sample 003 Lift work 004 Ctrl ZERO 1 005 Ctrl FILL A 1 006 Ctrl STEP MSM 753 007 Pump 240 s 008 Ctrl INJECT A 1 009 Move sample+1 010 Lift work 011 Ctrl ZERO 2 012 Ctrl Init 732 013 Scan Wait1 014 Ctrl FILL A 2 015 Ctrl Init 732 016 Pump 240 s 017 Ctrl INJECT A 2 018 Ctrl Init 732

Configuration/Control

 Initialize remote interface  Move needle to sample position  Place lift with needle to working position  Trigger autozero at 732/1 IC Detector  Switch injection valve A at 733 to "Fill"  Switch 753 suppressor module to next position  Fill sample loop A with sample during 240 s  Switch injection valve A at 733 to "Inject"  Move needle to sample position (+1)  Place lift with needle to working position  Trigger autozero at 732/2 IC Detector  Initialize remote lines at 732/1 and 732/2  Wait until 732/1 IC Det. sends signal on remote line 3  Switch injection valve B at 733 to "Fill"  Initialize remote lines at 732/1 and 732/2  Fill sample loop B with sample during 240 s  Switch injection valve B at 733 to "Inject"  Initialize remote lines at 732/1 and 732/2

3. Definition of new program parameters for the 723/1 IC Detector

Programm/Remote Configuration

under

Start_788 = **1*****

Reset_Start_788 = **0*****

4. To synchronize the second injection to the start of the second

chromatogram, a time program is started for the 732/1 IC Detector directly with the first injection:

1.1 Reset_Start_788

1.2 Flag end

1.0 Start_788

Both detectors 732/1 and 732/2 are registered in the system window

Setup/Start mode

under

in the field

'Start with inject

' .

4. By this procedure, with a delay of one minute, the 788 IC Filtration

Sample Processor receives a signal for processing the second sample. The total resulting delay (5 min.) is now independent from the time the sampler needs for rotating to the next sample. Therefore, the method for the second sample injection (e.g. method

cation

') gets a start delay under

Start delay: 5.0 min

Method Setup/Measure

788 IC Filtration Sample Processor

2 Installation

2.4.7 MIC-7

6.2128.130

Modular anion system with chemical suppression and dialysis

It is possible to operate the 788 IC Filtration Sample Processor in combination with an anion IC system with dialysis. For this purpose, the ultra-filtration cell of the 788 IC Filtration Sample Processor should be bridged (see

The 788 IC Filtration Sample Processor is connected to this anion IC system consisting of 732 IC Detector, 733 IC Separation Center (two injectors), 709 IC Pump, 753 IC Suppressor Module, 754 IC Dialysis Unit, and 762 IC Interface as shown in remote adapter, the 6.2125.090 remote connection cable and the

6.2134.080 RS232 cable.

section 2.3.10

6.2143.220

732

Fig. 16

6.2125.120

using the 6.2125.120

6.2125.090

762

6.2128.180

6.2134.100

6.2134.090

Abb. 16

6.2115.070

754

753

733

709

6.2141.110

6.2134.080

: Interconnection with anion IC system with chemical

suppression and dialysis (MIC 7)

788

788 IC Filtration Sample Processor

2.4 Installation for full control with «IC Net»

Settings in the «IC Net 2.1» program

There are two ways to operate an automated Metrohm IC system with dialysis:

A) For each sample, every analysis procedure contains first a dialysis

step followed by the chromatographic separation.



The total analysis time per sample results from summation of

the single times of dialysis and separation.

B) Chromatographic separation of one sample and dialysis of the next

sample is done simultaneously.



The total analysis time per sample is reduced to the time of

chromatographic separation.

Both techniques are described on the following pages.

A) Dialysis and separation of one sample in a single procedure

1. Definition of new program parameters for the 788 IC Filtration

Sample Processor under

PUMP 754 off = ---****0******

2. Program for the 788 IC Filtration Sample Processor:

001 Move sample 002 Lift work 003 Ctrl PUMP 754 on 004 Wait 120 s 005 Ctrl FILL B 1 / STEP 006 Wait 480 s 007 Ctrl ZERO 1 008 Ctrl STEP MSM 753 009 Wait 120 s 010 Ctrl FILL A 1 011 Ctrl INJECT B 1 012 Wait 30 s 013 Ctrl INJECT A 1 014 Ctrl PUMP 754 off

Configuration/Control

PUMP 754 on = ---****1******

 Move needle to sample position  Place lift with needle to working position  Start 754 Pump Unit  Rinse dialysis cell with sample solution  Stop of acceptor solution  Dialysis time  Trigger autozero at 732 IC Detector  Switch 753 suppressor module to next position  Additional dialysis time (total 600 s)  Switch injection valve A at 733 to "Fill"  Transfer acceptor solution to sample loop  Fill sample loop with acceptor solution for 30 s  Switch injection valve A at 733 to "Inject"  Stop 754 Pump Unit

788 IC Filtration Sample Processor

2 Installation

B) Separation with simultaneous dialysis of the next sample

The following time program descriptions are based on chromatogram times of 20 minutes. Approx. 10 minutes before end of each separation the dialysis of the next sample is started. For this purpose, the 788 program in the system dialysis.smt contains a waiting time of 540 s in line 006 (see below). These values have to be adapted, if necessary.

A main time program for the 788 IC Filtration Sample Processor is needed, which controls the both the injection of a dialyzed sample as well as the dialysis of the following sample: ('dialysis.smt').

The processing of the first and last sample of a queue requires time programs in additional system files. The 788 program in the system file 'start-dialysis.smt' just starts the dialysis of the first sample. 'enddialysis.smt' is used to inject the acceptor solution of the last sample.

dialysis.smt

1. Definition of new program parameters for the 788 IC Filtration Sample Processor under

Configuration/Control

PUMP 754 off = ---****0******

PUMP 754 on = ---****1******

2. Program for the 788 IC Filtration Sample Processor:

001 Ctrl FILL A 1 002 Ctrl INJECT B 1 003 Wait 30 s 004 Ctrl INJECT A 1 005 Ctrl PUMP 754 off 006 Wait 540 s 007 Move sample+1 008 Lift work 009 Ctrl PUMP 754 on 010 Wait 120 s 011 Ctrl FILL B 1 / STEP 012 Wait 540 s 013 Ctrl STEP MSM 753 014 Ctrl ZERO 1 015 Wait 60 s

start-dialysis.smt

3. Save the system file 'dialysis.smt' with a new name 'startdialysis.smt'.

 Switch injection valve A at 733 to "Fill"  Transfer acceptor solution to sample loop  Fill sample loop with acceptor solution for 30 s  Switch injection valve A at 733 to "Inject"  Stop 754 Pump Unit  Wait: 540 s  Move needle to sample position (+1)  Place lift with needle to working position  Start 754 Pump Unit  Rinse dialysis cell with sample solution  Stop of acceptor solution  Dialysis time  Switch 753 suppressor module to next position  Trigger autozero at 732 IC Detector  Additional dialysis time (total 600 s)

4. Remove the data recorder by clicking on the recorder icon with the right mouse button an selecting '

788 IC Filtration Sample Processor

unlink

2.4 Installation for full control with «IC Net»

5. Change this program for 'start-dialysis.smt' as follows and save it again:

001 Ctrl INIT 732 002 Move sample 003 Lift work 004 Ctrl PUMP 754 on 005 Wait 120 s 006 Ctrl FILL B 1 / STEP 007 Wait 540 s 008 Ctrl STEP MSM 753 009 Ctrl ZERO 1 010 Wait 60 s

End-Dialysis.smt

6. Open system file 'dialysis.smt', again. Select

Workplace

and save it under the new name 'end-dialysis.smt'.

7. Change the program for 'end-dialysis.smt' as follows and save it again:

001 Ctrl FILL A 1 002 Ctrl INJECT B 1 003 Wait 30 s 004 Ctrl INJECT A 1 005 Ctrl PUMP 754 off

 Initialize remote lines at 732 detector  Move needle to sample position  Place lift with needle to working position  Start 754 Pump Unit  Rinse dialysis cell with sample solution  Stop of acceptor solution  Dialysis time  Switch 753 suppressor module to next position  Trigger autozero at 732 IC Detector  Additional dialysis time (total 600 s)

Control/Connect to

8. The sample queue then contains the following entries, for example:

System Ident Vial

start-dialysis.smt Probe 0 1

dialysis.smt Probe 1 1

dialysis.smt Probe 2 2

dialysis.smt Probe 3 3

dialysis.smt Probe 4 4

dialysis.smt Probe 5 5

end-dialysis.smt Probe 6 6

Chrom. No. Dialysis No.

- 1 1 2 2 3 3 4 4 5 5 6 6 -

This example describes the processing of a queue with six samples. The columns 'Chrom. No.' and 'Dialysis No.' are just given for information. Please note that in the 788 program of 'dialysis.smt' not the given sample position 'Vial' is used; it is increased by 1 to start dialysis of the next sample. Therefore, all samples have to be arranged on the sample rack in a closed line.

788 IC Filtration Sample Processor

2 Installation

2.5 Installation with compact IC systems

2.5.1 Connection to 761 Compact IC or 790 Personal IC

Compact IC systems 761 Compact IC and 790 Personal IC are remote controlled by PC software. The 788 IC Filtration Sample Processor is connected to these IC systems via its remote connection:

761 / 790

788

Fig. 17

: Interconnection with compact IC systems

6.2141.110 cable

Tubing connections are described in section 2.10.2 of the instructions for use for the 761 Compact IC and 790 Personal IC, respectively.

The 6.2141.110 remote cable is needed for connecting the 788 IC Filtration Sample Processor to the compact IC system (761 or 790) as follows:

Electrical connection 788 – 761/790

x

Connect the end of the 6.2141.110 Cable marked with "766" to the

remote interface

x

Connect the end of the 6.2141.110 Cable marked with "732/1" to

788 IC Filtration Sample Processor.

3030

the remote interface of the 761 Compact IC and 790 Personal IC respectively.

x

The third end of this cable is not required with this configuration.

Settings at the 788 IC Filtration Sample Processor

x

For operation with the 761 Compact IC or 790 Personal IC it is recommended that the program "761" is used with the 788 IC Filtration Sample Processor (see

section 4.6.3

788 IC Filtration Sample Processor

2.5 Installation with compact IC systems

The corresponding settings in the PC software vary according to the IC system which is used:

Settings in the «761 Compact IC» program

A time program must be drawn up for the selected system which first produces an impulse at remote lead 3 to start sample changing on the 788 IC Filtration Sample Processor and then switches the injection valve to the "Fill" position. A further impulse is then produced to start filling the sample loop within 240 s. At the end the injection valve is switched to the "Inject" position, which also starts data acquisition.

Run conditions

In order that the joint operation of 761 – 788 functions properly the following conditions must be fulfilled:

x

The program must be started first on the 788 IC Filtration Sample Processor, then the "Sample Queue" on 761 Compact IC.

x

Remote lead 3 on the Compact IC must be set to 0 at the start of each determination (Set

System startup values: Remote line 3 = 0

788 IC Filtration Sample Processor

2 Installation

Settings in the «790 Personal IC» program

Run conditions

In order that the joint operation of 790 – 788 functions properly the program must be started first on the 788 IC Filtration Sample Processor, then the "Sample Queue" on 790 Personal IC.

788 IC Filtration Sample Processor

2.6 Installation for simple remote control of the IC system

Using the 714 PC Board or 771 IC Compact Interface a simple interconnection of modular IC systems to a PC can be established. These devices just convert the analog signals from the 732 IC Detector to digital data for the PC.

For simple control of the IC system, the 732 IC detector has to be connected to the PC additionally via its RS232 port. «IC Net» and the prior software «IC Metrodata» use this serial connection for a simple control of the IC system.

As described for compact IC systems the 788 IC Filtration Sample Processor is connected to these IC Systems by remote connection. Also, internal methods of the Sample Processor are used. In the present chapter, a description of the connection of the 788 IC Filtration Sample Processor to such IC Systems is given. The use of the corresponding 788 standard methods is given in

section 4.6

For this type of simple remote control of the IC System the 732 IC Detector is used as "Master".

Another version of PC connection for modular IC systems includes only signal transmission and conversion to digital data using the 714 PC Board or 771 IC Compact Interface. All methods and programs are directly edited at the 732 IC Detector or 788 IC Filtration Sample Processor. One of these instruments then holds the time control of the program and operated as "Master".

Examples for these different configurations are given in the following sections.

788 IC Filtration Sample Processor

2 Installation

2.6.1 IC system without suppression

The 788 IC Filtration Sample Processor is connected to an IC system without suppression consisting of 732 IC Detector, 733 IC Separation Center and 709 IC Pump as shown in cable. With this interconnection the standard methods

'SP'

and

'SP Seg'

can be used (see

732

Fig. 18

section 4.6

using the 6.2141.110

'PC', 'PC Seg'

733

6.2125.060 cable

709

788

6.2141.110 cable

Abb. 18: Interconnection with IC system without suppression

2.6.2 IC system with suppression with 788 as "Master"

The 788 IC Filtration Sample Processor is connected to an IC system with suppression consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump and either 752 Pump Unit or 753 Suppressor Module as shown in interconnection, in which the 788 IC Filtration Sample Processor is the "Master", the standard methods

section 4.6

Fig. 19

using the 6.2125.120 adaptor. With this

'SP'

732

and

'SP Seg'

can be used (see

6.2125.090 cable

6.2141.110 cable

6.2125.060 cable

752

753

6.2143.200 cable

6.2125.090 cable

Abb. 19

733

709

6.2125.120 adaptor

6.2143.210 cable

: Interconnection with IC system with suppression with

788 as "Master"

788 IC Filtration Sample Processor

2.6 Installation for simple remote control of the IC system

2.6.3 IC system with suppression with PC as "Master"

'PC', 'PC Seg'

and

Fig. 20

'Preconc'

using the 6.2125.120 adaptor. With this

can be used (see

732

section 4.6

6.2125.090 cable

6.2125.060 cable

752

753

6.2143.200 cable

788

6.2141.110 cable

Abb. 20

733

709

6.2143.210 cable

6.2125.120 adaptor

: Interconnection with IC system with suppression with

PC as "Master"

2.6.4 IC system for simultaneous anion/cation determination

The 788 IC Filtration Sample Processor is connected to an IC system for simultaneous determination of anions and cations consisting of two 732 IC Detectors, the 733 IC Separation Center, two 709 IC Pumps and (if suppression is used) the 753 Suppressor Module as shown in using the 6.2125.120 adaptor. With this interconnection the standard methods

'AnCat'

and

'AnCatSeg'

can be used (see

section 4.6

Fig. 21

6.2141.110 cable

788

6.2125.090 cable

788 IC Filtration Sample Processor

Abb. 21

732/1

733

709/1

Adapter 6.2125.120

732/2

6.2125.090 cable

709/2

6.2125.060 cable

753

: Interconnection for anion/cation system

6.2125.090 cable

6.2125.060 cable

6.2141.120 cable

6.2143.210 cable

2 Installation

2.6.5 IC system with sample dialysis

The 788 IC Filtration Sample Processor can be connected to an IC system with sample dialysis. However, this application is not planned for usage together with the ultra-filtration cell, which should be bridged.

The sample processor is connected to the modular IC system consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump, 754 Dialysis Unit and (if suppression is used) the 753 Suppressor Module as shown in interconnection the standard method

section 4.6

). If no suppression is used, the 754 Dialysis Unit can be

connected to the 6.2125.120 adaptor using the 6.2143.200 cable.

732

Fig. 22

using the 6.2125.120 adaptor. With this

'Dialysis'

can be used (see

788

6.2141.110 cable

Abb. 22

733

709

6.2125.090 cable

6.2125.060 cable

754

753

6.2143.220 cable

6.2125.120 adaptor

: Interconnection with IC system with dialysis

788 IC Filtration Sample Processor

2.7 Connection of devices to the RS232 interface

2.7.1 General information on RS232 interface

Many different instruments may be connected via the serial RS232

interface Metrohm remote control language (see serial interface (or parallel interface and parallel/serial converter) or a personal computer (PC) may be connected. Any other measuring instrument may be controlled via RS232 interface, as long as it supports serial data transmission.

. In addition to all Metrohm instruments that support the

4040

section 5.2

) any printer with

788

732

6.2125.060 cable

section 2.5.2

Fig. 23

Printer cables, see

: Connection possibilities for the RS232 interface

In order to guarantee safe data transmission, it is important to set the same RS232 interface parameters correctly for both instruments connected (see

section 2.5.2

Control commands (examples)

CTL:RS &M;$G CTL:RS &M;$S PRINT: config

starts a Metrohm instrument stops a Metrohm instrument prints a configuration report to a printer or PC

Scanning input data (example):

788 IC Filtration Sample Processor

SCN:RS : *R"

waiting for readiness of a Metrohm instrument

The settings and cables for the connection of a printer to the 788 IC Filtration Sample Processor are described in section 2.7.2.

2 Installation

2.7.2 Connecting a printer

Before connecting a printer to the RS232 interface, switch off the 788 IC Filtration Sample Processor !!

Printers with the following printer drivers may be connected:

IBM

IBM Proprinter and printers with IBM emulation

Epson

Epson printers and printers with Epson emulation

Seiko

Seiko printers DPU-411 and DPU-414

Citizen

Citizen printer IDP562-RS

HP printers and compatibles with HP PCL3 emulation

The interface parameters are set in the configuration submenu "

settings

". Corresponding parameters have to be set on the printer. The

following table provides information on a few selected printers.

Printer Cable RS232 settings Settings on printer

Seiko DPU-414

6.2125.130 baud rate: 9600

data bit: 8 stop bit: 1 parity: none handshake: Hws character set: Seiko

Citizen IDP562-RS

6.2125.050 baud rate: 9600

data bit: 8 stop bit: 1 parity: none handshake: Hws character set:Citizen

Epson LX-300 6.2125.050 baud rate: 9600

data bit: 8 stop bit: 1 parity: none handshake: Hws character set: Epson

Settings of the DIP switches:

Dip SW

Dip SW----1111 Dip SW

Dip SWDip SW 1111 OFF ON ON 2222 ON OFF ON 3333 ON ON ON 4444 OFF ON ON 5555 ON ON OFF 6666 OFF ON ON 7777 ON OFF ON 8888 ON OFF ON

Dip SW----2222 Dip SW

Dip SWDip SW

The switchable 7-bit ASCII character set of the printer will be automatically set to the national character sets in accordance with the set dialog language.

Settings of the DIP switches:

123456789

SSW1

The switchable 7-bit ASCII character set of the printer can be changed to the national character sets only by setting Dip switch 4 and 5:

444

OFF OFF USA ON ON Great Britain ON OFF France OFF ON Germany

Character set

555

Spanish does not have its own character set (it is best to select French).

see printer manual

Dip SW----3333

Dip SWDip SW

Character set

Character setCharacter set

>RS232

788 IC Filtration Sample Processor

2.7 Connection of devices to the RS232 interface

If you connect other printers, ensure that these emulate a printer mode supported by the 788 IC Filtration Sample Processor. Please note, that some printers with a GDI-Interface do not have such printer emulation. They require a direct control by a personal computer and therefore cannot be used with a 788 IC Filtration Sample Processor.

Most printers with a serial interface are connected using the 6.2125.050 cable. Printers with a parallel interface need a serial/parallel converter (e.g. 2.145.0300) and the 6.2125.020 Cable. The RS232 settings correspond to the data given in the table above. Only the setting for the printer driver (

character set:

) has to be adapted to the connected

printer or its printer emulation, respectively.

788 IC Filtration Sample Processor

2 Installation

788 IC Filtration Sample Processor

3.1 Prerequisites / Preparations

3 Operating tutorial

In order to become acquainted with the 788 IC Filtration Sample Processor and its mode of operation, it is helpful to work through the short Operating Tutorial. The basic operating steps that are required to prepare the first sample series and run it with a given method are described here.

For further explanations of the operation, please refer to section 4, which describes the functions of the individual keys and the programming in detail.

3.1 Prerequisites / Preparations

x

It is assumed that the 788 IC Filtration Sample Processor is fully installed (see and the plug cover.

x

Connect a 732 IC Detector at the remote connection

2.4

x

Prepare the ultra-filtration cell (see

x

Choose a simple IC method that you have saved in the 732 IC Detector or create a new simple method.

x

The <Ï> and <Ð> keys can be used to move the lift up or down.

x

Install the 6.2041.430 sample rack. Press <RESET> or <ENDSEQ> + <ENTER>. The 788 IC Filtration Sample Processor is initialized in this way with the lift and rack placed in the rest position. In this position the magnetic rack code can be read so that the internally stored rack data (position table, etc.) can be loaded. This should be done after every rack change.

section 2

). Don’t forget to mount the splash protection

section 2.3.8

RESET

CLEAR

ENDSEQ

3030

(see

section

788 IC Filtration Sample Processor

x

Insert some sample tubes into the sample rack, beginning with position 1. Using the keys <Í> and <Î> the rack can be turned for this purpose.

3 Operating tutorial

3.2 Configuration

x

The dialog language can be set in the configuration menu. Press

CONFIG

ENTER

x

and then <ENTER>.

configuration >auxiliaries

TOWER

SELECT

4 u <Ð>

<ENTER>

>auxiliaries dialog: english

x

This menu item has a colon, indicating that here the parameters can be selected from a list. Press <SELECT> several times in order to view the various selections and get used to this type of dialog.

>auxiliaries dialog: deutsch

x

With <ENTER> you can accept the suggestion

>auxiliaries display contrast: 3

x

By pressing <Ð> 4 times you reach the menu selection

way'

>auxiliaries max. lift way 125 mm

x

Here the lowest allowable lift position for automatic and manual

'dialog: english'

'max. lift

operation can be set. For the 6.2041.430 sample rack with

6.2743.050 sample tubes, this limit value of 125 mm should not be changed. Accept the value by pressing <ENTER>.

configuration >rack definitions

x

In order to put the 788 IC Filtration Sample Processor back into the

QUIT

initial position, press <QUIT> or <STOP>.

STOP

x

In the normal state, the method name and the sample counter reading are displayed in the first line. The second line serves as status

******** counter 0/127 PUMP- ready

line which displays the pump status and the changer status.

788 IC Filtration Sample Processor

3.3 Rack configuration

RESET

CLEAR

x

At the end of this basic configuration the 788 IC Filtration Sample Processor must be turned off and on again or re-initialized by pressing <RESET> to make the latest settings effective.

x

All data entered up to this point however, are retained. The same is true for any methods that may have been saved.

3.3 Rack configuration

x

Using the keys <Ï> and <Ð> you can run the lift to the desired work position for the needle.

CONFIG

<Ð>

<ENTER>

<Ð>

RESET

CLEAR

x

Now open the configuration menu with <CONFIG> and move the cursor key <Ð>, until you reach the submenu

tions'

. Press <ENTER> to open this submenu where you can de-

'>rack defini-

fine the rack configuration.

>rack definitions rack number 2

x

The rack number of the engaged rack will be displayed as soon as the sample rack has been correctly identified. By confirming with <ENTER> you access the rack data. (By entering another rack number you can also edit the data of a sample rack that is not engaged.)

x

You can skip the first entries (code and rack type) with the cursor key <Ð>. Now you can enter the work position of the needle.

>rack definitions 2 work position 125 mm

x

Because you have already positioned the needle at the desired height, you can accept the current lift position directly by pressing <CLEAR>. Of course the work position can be entered manually or the value that has automatically been accepted can be modified later. Lift positions are given in millimeters (0…125 mm), measured from the uppermost limit (rest position) of the lift. Consider during the input of the working position that with sample tubes sealed with non-perforated PE caps the work position must be set to 125 mm, since otherwise a vacuum can develop in the sample tube and the sample will not be aspirated correctly.

788 IC Filtration Sample Processor

3 Operating tutorial

<ENTER>

>rack definitions 2 work position 71 mm

x

In any case don’t forget to confirm the value with <ENTER>.

>rack definitions 2 rinse position 125 mm

x

The next menu item

'rinse position'

defines the height at which the lift must be when the needle is rinsed. As for the work position, the value here can also be entered manually or automatically accepted. For the latter, the configuration menu must be exited by pressing <QUIT> twice and the lift newly positioned.

>rack definitions 2 rinse position 105 mm

>rack definitions 2 shift position 0 mm

x

The menu item

'shift position'

defines the height of the needle when the sample rack is rotated. For the 788 IC Filtration Sample Processor this height is set to 0 mm and cannot be changed.

x

Press <ENTER>.

<ENTER>

STOP

>rack definitions 2 special position 0 mm

x

'special position'

The

defines a further height of the lift. For the

entry proceed as for the work position.

>rack definitions 2 special position 55 mm

x

The final entry in the rack configuration is the definition of the position of the special beakers.

>rack definitions 2 >>special positions

x

In the submenu

'>>special positions'

enter the positions at which you have placed conditioning or rinsing beakers (for the 6.2041.430 sample rack the two positions

cial beaker 2 129'

x

The configuration can now be exited with <STOP> or by pressing

are already set).

'special beaker 1 128'

and

<QUIT> three times. The rack data entered are now available at all times and must not be re-defined every time.

'spe-

788 IC Filtration Sample Processor

3.4 Methods

x

USER

METHOD

<ENTER>

Now open the user method menu by pressing <USER METHOD>.

methods >recall method

x

Press <ENTER> to load a predefined method.

TOWER

SELECT

<ENTER>

x

Choose with the 788 IC Filtration Sample Processor as "Master" from which you can learn the basic sample changer commands.

x

After you have confirmed loading the method with <ENTER>, the name of the method appears in the upper left corner of the display. You can now use the TRACE function to run the method in steps to understand how it works (see

3.5 "Tracing"

>recall method method: 

'SP'

with the <SELECT> key. This is a universal method

section 3.5





SAMPLE

<2>

<ENTER>

PARAM

<3>

<ENTER>

x

Before you begin tracing, set the position of the first sample with the SAMPLE command. Press <SAMPLE>.

manual operation SAMPLE: = 1

x

Press <2> and <ENTER>.

x

Now press <PARAM> to open the parameter menu. All parameters and sequences that are stored with methods can be found here.

parameters number of samples: rack

x

The first menu entry defines the number of sample tubes (without the special beakers) that are to be treated in a series. Here you can choose between

'rack'

(= a sample rack that is partially or completely filled, only positions with a sample tube are counted) and (= infinite number of samples) with <SELECT>. However for this

'3'

learning sequence, enter

on the keyboard. It is also possible here, as with the other parameters, to enter data manually or use the "select" choice.

'*'

788 IC Filtration Sample Processor

3 Operating tutorial

<ENTER>

<Ð>

START

<QUIT>

parameters >start sequence

x

In the submenu

'>start sequence'

the commands that are exe-

cuted at the start of a sample series are found.

>start sequence 1 CTL:Rm: INIT

x

The first command is a CTL command for the initialization of the remote interface. This command should be used in the start sequence of every method. Do not change anything here and press <Ð>.

>start sequence 2 CTL:Rm: PUMP 752 ON

x

With the second CTL command the 752 Pump Unit used for the operation of the suppressor is started. If you use a suppressor and the 752 Pump Unit is connected to the remote interface of the 788 IC Filtration Sample Processor, press <START>. The 752 Pump Unit is started.

x

Leave this submenu with <QUIT>.

<Ð>

<ENTER>

START

<START>

x

In the submenu

'>sample sequence'

you find the command sequences that are executed for every sample. It is recommended to test out this procedure line by line with the TRACE function.

>sample sequence 1 SCN:Rm : Pump1 ?



x

In the first line the SCAN command is used to scan the status of the 709 IC Pump. The 788 IC Filtration Sample Processor waits until the pump drive of the 709 IC Pump is switched on. Therefore switch on the pump drive at the 709 IC Pump. If you press <START> at this point, this command is executed and the next program line appears.

>sample sequence 2 MOVE 1 : sample

x

Press <START> to place the sample tube in the position predefined as sample position 2 below the needle.

>sample sequence 3 LIFT: 1 : work mm

x

On the next line press <START> again to move the needle into the work position you previously defined for this rack.

x

With this command you can become acquainted with the LEARN mode. It allows the user to manually set the parameters of a command on a trial basis.

788 IC Filtration Sample Processor

3.5 "Tracing"

LEARN

HOLD

<ENTER>

<START>

LEARN

HOLD

LEARN

HOLD

<ENTER>

x

Press <LEARN> to access the LEARN mode. The blinking LEARNLED indicates that the 788 IC Filtration Sample Processor is ready to execute the command.

x

Now move the lift into the desired position with the <Ð> and <Ï> keys. You will notice that the current lift position is always indicated "live". During execution of the command the LEARN-LED is lit continuously. Accept the lift position that has been set by pressing <ENTER> and thereby exit the LEARN mode. The LEARN LED goes off again.

>sample sequence 4 CTL:Rm: FILL A 1

 x

In this line the injection valve at the 733 IC Separation Center is switched to the "Fill" position.

>sample sequence 5 PUMP 1.1 : 240 s

x

In this line the peristaltic pump at the 788 IC Filtration Sample Processor is started in order to convey the sample from the sample tube to the sample loop at the 733 IC Separation Center.

x

Here, you can use the LEARN mode to optimize the pump time too.

x

In this case, as with the other "teachable" commands (the LIFT command is an exception), pressing the <LEARN> key causes immediate execution of the corresponding command. The elapsed time is also displayed here "live". By pressing the <LEARN> key again the command can be interrupted.

x

The blinking LED indicates that the 788 IC Filtration Sample Processor is still in the LEARN mode. If you now switch the pump back on with the <LEARN> key, you will see that the "live" value (pump time) is now added to the existing value.

x

Now set the pump time in this way. Accept the total time with <ENTER> and exit the LEARN mode in this way.

<START>

788 IC Filtration Sample Processor

>sample sequence 6 CTL:Rm: ZERO 1

x

In this line the autozero function at the 732 IC Detector is triggered.

>sample sequence 7 CTL:Rm: INJECT A 1

x

In this line the injection valve at the 733 IC Separation Center is switched to the "Inject" position.

3 Operating tutorial

<ENTER>

<QUIT>

<Ð>

<ENTER>

START

QUIT

>sample sequence 8 WAIT 1200 s

x

In this line a waiting time is defined that is used for the acquisition of the chromatogram.

x

The LEARN mode can also be used with the WAIT command.

>sample sequence 9 NOP

x

An empty line with a

'NOP'

-entry (no operation) always forms the

end of a sequence.

x

Exit the sample sequence with <QUIT> and go to the final sequence.

x

After all sample tubes have been processed, the final sequence is executed.

>final sequence 1 CTL:Rm: PUMP R/S 1

x

In this line the pump drive at the 709 IC Pump is switched off. Press <START>.

>final sequence 2 CTL:Rm: PUMP 752 OFF

x

In this line the pump drive at the 752 Pump Unit is switched off. Press <START>.

>final sequence 3 NOP

x

Now you have reached the end of the final sequence and have completed the entire run of a sample series.

x

By pressing <QUIT> twice the 788 IC Filtration Sample Processor returns to the normal state.

x

Now prepare some sample tubes and place them on the sample rack. Prepare the IC system with 732 IC Detector, 733 IC Separation Center, 709 IC Pump and 752 Pump Unit for the recording of chromatograms.

x

Enter the number of samples to be processed (<PARAM>) and define the position of the first sample (SAMPLE = 1).

PARAM

SAMPLE

START

x

Now you can start your first sample series with <START>.

788 IC Filtration Sample Processor

4.1 Fundamentals of operation

4 Operation

This section provides a detailed description of the operation of the 788 IC Filtration Sample Processor using the keyboard and dialog display. The overview of the fundamentals of operation and key functions (section 4.1) is followed by a detailed description of configuration settings (section 4.2), methods (section 4.3), manual operation (section 4.4), sample racks (section 4.5), standard methods (section

4.6), and filtration principles (section 4.7).

4.1 Fundamentals of operation

4.1.1 Display

The display on the 6.2142.010 keyboard consists of two lines, each having 24 characters.

The first line serves as a title line in which the current method and the sample counter reading are displayed. In the edit mode the menu title is shown.

The second line serves as a status line which displays specific activities depending on the operating state. In the edit mode it serves as an entry line.

Normal State

Method name

Pump status

Method processing

Sample counter

ÎÎÎÎ

******** counter 1/127 PUMP- ready

ÎÎÎÎ

Sample counter

ÐÐÐÐ

ÍÍÍÍ

Changer status

Running sequence

Edit mode

Menu line/command

788 IC Filtration Sample Processor

Method name

Menu title

ÎÎÎÎ

******** counter 2/127 START 03 WAIT 11 s

ÎÎÎÎ

ÏÏÏÏ

Current command with line number

ÎÎÎÎ

>sample sequence 1 MOVE 1 : sample

ÎÎÎÎ

Parameter

ÍÍÍÍ

Parameter

ÍÍÍÍ

Parameter

4 Operation

4.1.2 Keyboard

Metrohm

SC Controller

MetrohmMetrohm

******** counter 1/999 PUMP- ready

TOWER 1 TOWER 2 LEARN

CONFIG

HOME

INSERT

DELETE QUIT ENTER

6.2142.010

PARAM

TOWER

SELECT

USER

METHOD

END

RESET

CLEAR

SAMPLE

PUMP

4 5

SCAN

DEF

LEARN

HOLD

MOVE

STIR DOS

CTRL

ENDSEQ

STARTSTOP

LIFT

WAIT

Most of the keys have two functions according to whether the 788 IC Filtration Sample Processor is in the normal state or in the edit mode.

The uppermost row contains the keys which make the menus accessible (<CONFIG>, <PARAM>, <USER METHOD>). Here with the help of the keys on the left side of the keyboard, you can navigate and change parameters. For the latter, the numerical keypad on the right half of the keyboard is available. Except for the menu

'methods'

tries under these selection menus can be altered while a method is in process and for the most part, have an immediate effect on the procedure which is running.

The lowermost row of keys (<HOLD>, <STOP>, <START>) is used for the direct control of method processing.

Except for editing '5' and '6' the keys <5/STIR> and <6/DOS> don't have any additional functions with this instrument.

788 IC Filtration Sample Processor

the en-

4.1 Fundamentals of operation

4.1.3 Overview of key functions

Key Normal state Edit mode

CONFIG

PARAM

Open the configuration menu

x

The <CONFIG> key opens the selection menu for the configuration of the 788 IC Filtration Sample Processor .

x

The settings in the configuration menu remain constant until they are changed or the working memory (RAM) for the configuration settings is re-initialized.

Open the parameter menu

x

The <PARAM> key opens the selection menu for the changer settings.

x

All settings that are set in the parameter menu belong to a method and will be saved with the method. These parameters are method-specific.

Select configuration settings

x

When the configuration menu is open, pressing the <CONFIG> key displays the next menu line.

x

After the last line is displayed, the first one follows.

x

<QUIT> exits the menu.

Select method parameters

x

When the parameter menu is open, pressing the <PARAM> key displays the next menu line.

x

After the last line is displayed, the first one follows.

x

<QUIT> exits the menu.

USER

METHOD

HOME

Open the user method menu

x

The <USER METHOD> key opens the selection menu for the loading, saving and deletion of user-defined methods.

Bring lift to zero-position

x

The <HOME> key runs the lift with the needle to the zero-position (0 mm), i.e. to the upper stop.

Select method functions

x

When the user method menu is open, pressing the <USER METHOD> key displays the next menu line.

x

After the last line is displayed, the first one follows.

x

<QUIT> exits the menu.

Select the first line of a menu

x

With the <HOME> key, the first line in a menu or a sequence can be accessed.

x

Any data that has been altered in a menu or command line is not carried over (see <ENTER> key).

788 IC Filtration Sample Processor

4 Operation

Key Normal state Edit mode

END

Lift in work position

x

The <END> key runs the lift into the work position.

x

The work position is defined separately for every sample rack in the configuration menu under

'>rack definitions'

the rest position, i.e. as measured from the upper stop).

Run lift upwards

x

Run lift upwards. The lift movement is executed as long as the key remains pressed.

(in mm from

Select the last line of a menu

x

With the <END> key, the last line in a menu or a sequence can be accessed.

x

Any data that has been altered in a menu or command line is not carried over (see <ENTER> key).

Select previous menu line

x

In a Select menu or a sequence the <Ï> key accesses the previous line.

x

Any data that has been altered in a menu or command line is not carried over (see <ENTER> key).

Run lift downwards

x

Run lift downwards. The lift movement is executed as long as the key remains pressed.

Turn rack left

x

The <Í> key turns the sample rack one position to the left, i.e. in the counterclockwise direction. The next highest beaker position is placed under the needle.

x

The turning speed of the rack can be defined in the Parameter menu.

x

The rotation of the rack can only be carried out when the lift is at or above the shift position.

Select next menu line

x

In a Select Menu or a sequence the <Ð> key accesses the next line.

x

Any data that has been altered in a menu or command line is not carried over (see <ENTER> key).

Move the cursor one column to the left

x

With the <Í> key the cursor is moved one column to the left in an edit line with two parameters.

x

Any altered data will not be carried over during this action (see <ENTER> key).

788 IC Filtration Sample Processor

4.1 Fundamentals of operation

Key Normal state Edit mode

INSERT

Turn rack right

x

The <Î> key turns the sample rack one position to the right, i.e. in the clockwise direction. The next lowest beaker position is placed under the needle.

x

The turning speed of the rack can be defined in the Parameter menu.

x

The rotation of the rack can only be

Move the cursor one column to the right

x

With the <Î> key the cursor is moved one column to the right in an editing line with two parameters.

x

Any data which has been altered will not be carried over during this action (see <ENTER> key).

carried out when the lift is at or above the shift position.

Add a command line to a

sequence

x

Adds a new command line

the current line

NOP

The

command (no operation)

in a sequence.

automatically occupies this line and has no function.

x

The lines following this line are shifted one line downwards.

above

DELETE

TOWER

SELECT

Delete a command line in a

sequence

x

Deletes the current line in a sequence.

x

The lines which follow shift upwards by one line.

Select parameter

x

With the <SELECT> key given data values can be selected for a particular parameter in manual operation.

x

With every repeated keystroke the next value that can be selected is displayed. The last value is followed again by the first.

x

The data is accepted with <ENTER>.

788 IC Filtration Sample Processor

4 Operation

Key Normal state Edit mode

RESET

CLEAR

QUIT

Initialization of the 788 IC Filtration Sample Processor

x

The <RESET> key serves to initialize the IC Sample Processor.

x

A method in the working memory remains unchanged. The sample rack and the lift return to their initial positions in this case.

Interruption of a method after the current sequence

x

During processing of a method, the sample series can be aborted with <CLEAR> so that the sample currently being processed is processed to completion. The final sequence is not executed in this case.

Aborting a command already in operation

x

When a sequence is being processed the currently running command will be aborted and continued in the course of the next command line. This is useful when a programmed waiting time should be shortened or when a signal cannot be recorded with a SCAN command.

Quitting error message

x

With the <QUIT> key error messages can be acknowledged. Before acknowledging error messages, the cause should be remedied.

x

The command during which the error message occurs will nevertheless be carried out (during manual operation).

x

If an error occurs during method processing the error message is acknowledged by pressing the <QUIT> key and the method is interrupted (HOLD status). Afterwards <START> can be used to continue with the following command line or <STOP> will halt processing.

Deleting parameters, setting the default values

x

The <CLEAR> key sets the initial (default) value given for a parameter.

Delete last character

x

In text edit mode the last character will be deleted with <CLEAR> (Backspace).

Abort entry, select next highest menu level

x

With the <QUIT> key the active (sub)menu or a menu or command line is exited. The next highest menu level or the basic state is selected.

x

Any data from a menu or command line which has been changed will not be taken over in this case. This will be signaled by an acoustic signal (see <ENTER> key).

x

<QUIT> acknowledges error messages.

788 IC Filtration Sample Processor

4.1 Fundamentals of operation

Key Normal state Edit mode

ENTER

SAMPLE

Set sample position

x

The <SAMPLE> key serves to set the current sample position.

x

When starting a method, this position is assumed to have the first sample of a series.

x

If the current sample position is not manually set before the start of a sample series, rack position 1 is always started first.

Accept data, next line

x

The <ENTER> key accepts the value entered and selects the next menu line.

x

A modification of data or parameters must

always

be confirmed by <ENTER>, otherwise the change will not be accepted.

x

If a change in a parameter is not confirmed by <ENTER> and another menu line is accessed, the previous value will be retained. This is indicated by an acoustic signal.

Numerical entry ('7')

Set sample position

x

In a start sequence the SAMPLE command serves to define the first sample of a sample series.

x

If no SAMPLE definition is made in any sequence, the manually set rack position is assumed to be the first sample.

MOVE

Position sample tube

x

Turn the sample rack to position the described sample tube under the needle. In addition to the predefined sample tubes, the eight rackspecific special beakers available can be placed. Absolute positions can also be chosen.

x

The turning speed can be altered in the Parameter menu.

Numerical entry ('8')

Position sample tube

x

Turning the sample rack to position the described sample tube under the needle. In addition to the predefined sample tubes, the 8 rack specific special beakers available can also be placed. Absolute positions can also be chosen.

x

The turning speed can be altered in the Parameter menu.

788 IC Filtration Sample Processor

4 Operation

Key Normal state Edit mode

LIFT

PUMP

Positioning the lift

x

Raises or lowers the lift to a predefined position. These positions (work position, rinse position, shift position, special position) can be defined in the Configuration menu as rack-specific.

x

An absolute lift position in mm can also be given.

Control pump

x

The <PUMP> key is used to switch the peristaltic pump of the 788 IC Filtration Sample Processor on or off.

x

The state of the pump is shown in the second display line in the normal state.

'PUMP+'

"switched on", "switched off").

means

'PUMP–'

means

Numerical entry ('9')

Positioning the lift

x

The lift can be run to the predefined positions (work position, rinse position, shift position, special position) in a sequence.

x

An absolute lift position in mm can also be given.

Numerical entry ('4')

Control pump

x

The pump can be specifically switched on and off in a sequence or set to operate for a fixed amount of time (in seconds).

SCAN

Display input signals

x

Display of the incoming signal or data on the remote or RS interface. This function serves to control the data communication or states of connected instruments.

x

The first parameter represents the interface selection. The signals for data being received at that moment are displayed as the second parameter.

x

If the parallel remote interface ( is selected, the signal states of the incoming remote lines are displayed in binary form (

active, see

x

If the serial RS232 interface (

= line inactive). Details

section 5.1

selected, the character string being received is displayed line by line (14 characters). Details see

5.2

= line

)

) is

section

Numerical entry ('1')

Scan input signals

x

In a sequence the SCAN command causes method processing to stop until the predefined bit pattern (with the remote interface) or the given character string (with the RS232 interface) is received.

x

Predefined bit patterns are available for the remote interface and can be selected via simple short-names (e.g.

'End 2'

x

Character strings consisting of 14

'Ready 1'

ASCII characters may be defined with the RS232 interface.

788 IC Filtration Sample Processor

4.1 Fundamentals of operation

Key Normal state Edit mode

CTRL

Interface control

x

Controlling of external instruments via the remote and RS232 interface. This function is used for data communication with or control of connected instruments.

x

The first parameter stands for the selection of the interface. The second parameter defines the state of the remote output lines or data to be transmitted via RS232 interface.

Parameters for remote interface

x

Bit pattern with 14 digits ( for the 14 output lines or predefined patterns accessible by the <SELECT> key (

A 1'

, etc.).

'INIT 732', 'FILL

Parameters for RS232 interface

x

Character string with up to 14 alphanumerical characters. Default value:

'&D.S"9"'

, may be set with <CLEAR>. Most Metrohm instruments can be controlled with such remote control commands, see

section 5.2

0, 1

or *)

Numerical entry ('2')

Interface control

x

Setting the 14 signal lines of the remote interface or sending a character string via the RS232 interface to control instruments connected.

x

Predefined bit patterns are available for the remote interface and can be selected via simple short-names (e.g.

'FILL A 1'

x

Character strings consisting of 14

'INIT 732'

ASCII characters may be defined with the RS232 interface.

WAIT

DEF

Numerical entry ('3')

Define waiting time

x

Waiting for a certain time interval to elapse in a sequence.

Print report

x

The <PRINT> key serves for the manual printout of reports.

The choice of printer type and the parameters of the RS232 interface must be done in the Configuration menu under

'>RS232 settings'

Numerical entry ('0')

Numerical entry ('.')

Text entry

x

In a menu or sequence line in which entry of text is required, (e.g. method name), text edit mode is activated with "<".

x

Existing text is deleted in this event and the text cursor is set on the left margin of the text field.

788 IC Filtration Sample Processor

4 Operation

Key Normal state Edit mode

x

"<" serves also for shifting the chain of characters to chose from, i.e. the text cursor is shifted one place to the left for each instance

section 4.1.6

(see

ENDSEQ

Initialization

x

The 788 IC Filtration Sample Processor is initialized with the <ENDSEQ> key followed by <ENTER>.

x

A method in the working memory remains unchanged. The rack and the lift are run to the initial position.

Text entry

x

In a menu or sequence line in which entry of text is required, (e.g. method name), text edit mode is activated with ">". Existing text remains in this event and the text cursor is set at the end of the existing character string.

x

">" serves also for shifting the chain of characters to chose from, i.e. the text cursor is shifted one place to the right for each instance

section 4.1.6

(see

Set end mark

x

For test purposes an <ENDSEQ> command can be placed at any line desired in a sequence. This has the effect that the sequence is only executed up to this end mark.

START

Start a method

x

The <START> key starts a method. Starting is only possible when the IC Sample Processor is in the normal state, i.e. when is shown in the display.

x

When starting a sample series, the sample counter is set to 0.

x

When <START> is pressed after an interruption (<HOLD>), the sequence is continued with the next command line.

'ready'

Trace function

x

During editing of a sequence, the command defined in the command line can be directly executed with the <START> key.

x

A sequence can therefore be tested from start to finish (or in parts) in single steps ("tracing").

788 IC Filtration Sample Processor

4.1 Fundamentals of operation

Key Normal state Edit mode

STOP

Stop process and peripheral instruments

x

The <STOP> key terminates a method.

x

Any peripheral instruments that are connected (e.g. 709 IC Pump) are not automatically stopped. In

'>manual stop options'

menu of the Parameter menu, you can specify what signal or data is to be transmitted via the interface involved (remote or RS232) during manual activation of the <STOP> key. The connected instrument can be halted or if necessary, initialized

section 4.3.2

(see

x

During a manual halt of a sample

series with <STOP>, the end sequence of the method will not be executed.

x

In the normal state the <STOP> key also stops the pump. The

'>manual stop options'

nected peripheral instruments are also effective in the normal state.

, a sub-

for con-

Stop editing

x

<STOP> causes the editing to abort and the instrument to return to the normal state. (exception: Process sequences).

LEARN

HOLD

Interrupt Process

x

The <HOLD> key interrupts the processing of a method. However connected peripheral instruments (e.g. 709 IC Pump) are not halted. Only method processing is interrupted. In the "HOLD" state a method can be completely halted with <STOP> or continued with the next command in line by pressing <START>.

x

After quitting an error message during method processing the IC

Switch on LEARN mode

x

The <LEARN> key serves to start the LEARN mode. This mode is provided for easier editing of process sequences. It allows direct acceptance of a parameter value that has been set by manual control. The LEARN mode is available for the following commands:

LIFT, PUMP, SCN, WAIT

x

Details concerning the LEARN mode see

section 4.3.4

Filtration Sample Processor automatically goes into the "HOLD" state.

788 IC Filtration Sample Processor

4 Operation

4.1.4 Instrument dialog

The instrument dialog of the 788 IC Filtration Sample Processor is organized in the form of so-called rolling inquiries which are arranged in menu levels in a hierarchical manner and are subject to the following rules:

Main menu

The function keys <CONFIG>, <PARAM> and <USER METHOD> of the 788 IC Filtration Sample Processor open a main menu whose thematically arranged submenus are selected by repeated pressing of this key or with <Ð>. The name of the key always appears in the top line of the display.

Submenu

Each submenu has a title marked by line of the display. <ENTER> is used to move from the title to the individual inquiries, which are used to change the most important settings of the instrument. The top line of the display always shows the title of the active submenu.

">"

which appears in the bottom

Navigating within the menus is accomplished with the cursor keys; <HOME> jumps to the first, <END> to the last menu line.

<QUIT> exits the active menu, opens the next highest menu level or returns to the normal state.

<ENTER> either opens a submenu or confirms data entry at the lowermost menu level.

Inquiries

In inquiries without

":"

, the values must be entered using the numeric keys. <ENTER> is used to accept the inputted value and the next inquiry appears.

In the case of inquiries with

":"

, the admissible values can be selected with the <SELECT> key>. <ENTER> is used to accept the set value and the next inquiry appears.

Depending on the parameter, <CLEAR> is used to reset the displayed value to the smallest possible value or the default value. The <CLEAR> key also serves to abort wrong entries.

<QUIT> is used to quit the inquiries and return to the main menu.

You will find a schematic representation of the instrument dialog in

Fig. 24

788 IC Filtration Sample Processor

4.1 Fundamentals of operation

Normal state

KEY

QUIT

Kex title >Submenu 1

KEY

Key title >Submenu 2

KEY

ENTER

>Submenu 1 Inquiry 1

ENTER

>Submenu 1 Inquiry 2

>Submenu 1 Inquiry n

>Submenu 2 Inquiry 1

ENTER

QUIT

Key title >Submenu n

Fig. 24

: Schematic representation of the instrument dialog

KEY

ENTER

>Submenu 2 Inquiry 2

>Submenu 2 Inquiry n

788 IC Filtration Sample Processor

4 Operation

4.1.5 Data entry

Edit line

In a menu line or a sequence one or two parameters respectively can be entered. A blinking block cursor indicates where a parameter can be entered.

>changer settings lift rate 1 Ű12 mm/s

Ï Ï Ï

menu item cursor parameter

>sample sequence 7 PUMP 1.1 : Ű150 s

Ï Ï Ï

command 1st param. 2nd param.

The cursor keys<Î> and <Í> can be used to switch between the parameters. Pressing <ENTER> shifts the cursor automatically to the right, pressing <QUIT> correspondingly to the left.

title line

edit line

title line

edit line

<Select> choices (Roll-up selection)

TOWER

SELECT

Data can usually be entered directly via the numerical keypad block on the keyboard. Pressing <SELECT> at entries which are specially identified by a colon displays a preset selection of data. This selection is cyclic, structured like a revolving drum.

Example

>RS232 settings character set: IBM

IBM

Epson Seiko

Citizen

<ENTER>

>RS232 settings character set: Epson

788 IC Filtration Sample Processor

4.1 Fundamentals of operation

4.1.6 Text entry

The text editor can be used when text entry is provided.

Numbers can be entered directly via the keyboard.

Opening the text editor

ENDSEQ

The keys "<<<<" or ">>>>" open the text editor. With "<<<<" an existing character

string is deleted and the text cursor is set to the left margin of the edit line. With ">>>>" an existing character string remains and the text cursor is set on the last character of the existing text.

A character chain is displayed that is composed of all the characters in alphabetical order that can be entered. The blinking character is the currently selected one (text cursor).

Character selection

The keys "<<<<" and ">>>>" move the character chain composed of all

possible characters (capital and small letters, numbers and special characters, in alphabetical order) in the desired direction underneath the text cursor. Pressing these keys once has the effect that the

character chain is shifted one position left or right. The character chain can be shifted quickly by pressing the keys longer.

ENTER

RESET

CLEAR

QUIT

Confirmation of the character selection

The <ENTER> key causes the character that is currently positioned at the text cursor to be appended to the existing text. When the entire width of the edit line is filled, text edit mode is left and the text is accepted with <ENTER>.

Delete character

The <CLEAR> key deletes the last character of the existing text line. The text cursor automatically shifts one character to the left.

Exit text entry

With <QUIT> the text edit mode is exited. The text line displayed can be accepted with <ENTER> or rejected by pressing <QUIT> a second time.

788 IC Filtration Sample Processor

4 Operation

Scheme

>store method method: ********

"<" or ">"

blinking text cursor

>store method method:

<ENTER>

>store method method:

>store method method: Chloride

àààà

ABCDEFGHIJ...abcd...1234...*%&/()=...

àààà

DEFGHIJ...abcd...1234...*%&/()=...

<QUIT>

<ENTER>

blinking text cursor

"<" or ">"

...<ENTER>...

<QUIT>

character chain

2x ">"

reject

An entire text line can be entered in this way, for example, for the naming of a method. Text editing can be ended with <QUIT>. The text line will be displayed in its entirety and can be accepted with <ENTER> or rejected with <QUIT>.

788 IC Filtration Sample Processor

4.2 Basic settings

4.2.1 Configuration – <CONFIG> key

The <CONFIG> key is used for the entry of basic settings of general

CONFIG

configuration >auxiliaries

applicability. If you have changed the configuration, press <RESET> or switch on and off the instrument to make the entries effective. The <CONFIG> key opens the following main menu:

Various general instrument settings

configuration >auxiliaries

>auxiliaries dialog: english

>auxiliaries display contrast 3

configuration >rack definitions

configuration >RS232 settings

Repeated pressing of the <CONFIG> key selects the submenus in turn. Use <Ð> or <Ï> to move up or down one menu item; use <HOME> or <END> to move to the first or last menu item respectively. The submenu is opened with <ENTER>, <QUIT> returns to the normal state. The following listings show all dialog items which appear under <CONFIG>. The values shown in the display are the default values, the possible entry values or ranges are shown below the display.

General instrument settings

english,deutsch, français,español

0}7

Sample rack settings

Settings for RS232 interface

Dialog language

english

English

deutsch

français

español

German French Spanish

Display contrast

= large contrast

= weak contrast

788 IC Filtration Sample Processor

4 Operation

>auxiliaries beeper: ON

ON,OFF

Acoustic warning signal

Acoustic warning signal in case of error messages

OFF

No warning signal

>auxiliaries device label

8 ASCII characters

Device label

Freely selectable ASCII character string for characterizing the instrument (text entry, see

section 4.1.6).

>auxiliaries program 5.788.0010

read only

Number of the program version

Display only (no entry possibility).

Please specify this number in inquiries to Metrohm.

>auxiliaries max. lift way 125 mm

0}125 mm

Maximum stroke path for needle

The following entry becomes effective after a RESET or switching the IC Sample Processor off and on again.

This setting for max. lift way is

important

for safe operation. If the value for this entry is correct, damage to the needle can be avoided because this prevents the needle from being driven lower than the position indicated. If you use the 6.2041.430 sample rack with

6.2743.050 sample tubes, the default value of 125 mm should be retained.

Pressing <CLEAR> directly accepts the current lift position of the active tower.

configuration >rack definitions

>rack definitions rack number 2

>rack definitions 2 code 010001

Definition of sample racks

Number of the rack

The number of the rack in position is automatically dis-

1}16

played here, when its configuration is already stored and when a RESET has been performed. If the configuration of another rack must be changed, its rack number must be entered and confirmed with <ENTER>. The rack number will be shown in the first menu line for the subsequent entries. For more information concerning sample racks, see signed to the 6.2041.430 sample rack delivered.

Identification code of the rack

The rack code must be unique and can only occur once

6 bits

in the instrument (see

section 4.5

. The number

is as-

788 IC Filtration Sample Processor

4.2 Basic settings

>rack definitions 2 type: M129-2

M129-2}}}}

Type description of the rack

<SELECT> enables the choice of Metrohm-specific and self-defined rack types (see

section 4.5

>rack definitions 2 work position 125 mm

0}125 mm

Working position of the needle

in mm from the upper stop. Pressing <CLEAR> directly accepts the current lift po-

sition.

>rack definitions 2 rinse position 125 mm

0}125 mm

Rinsing position of the needle

in mm from the upper stop. Pressing <CLEAR> directly accepts the current lift po-

sition.

>rack definitions 2 shift position 0 mm

0 mm

Shifting position of the needle

in mm from the upper stop. Only the value

'0'

is permissible.

>rack definitions 2 special position 0 mm

0}125 mm

Special position of the needle

in mm from the upper stop. Pressing <CLEAR> directly accepts the current lift po-

sition.

>rack definitions 2 >>special positions

Submenu

Special positions submenu

>>special positions special beaker 1 128

0}number of positions

Position of special beaker 1

>>special positions special beaker 2 129

0}number of positions

etc. up to special beaker 8

Position of special beaker 2

Up to 8 special beaker positions can be defined (see

section 4.5

788 IC Filtration Sample Processor

4 Operation

configuration >RS232 settings

>RS232 settings baud rate: 9600

>RS232 settings data bit: 8

>RS232 settings stop bit: 1

>RS232 settings parity: none

Settings for RS232 interface

For further details on the RS232 interface, see

9600,4800,2400,

1200,600,300

7,8

1,2

none,odd,even

section 5.2.

Data transmission rate (baud rate)

Data transmission rate in bit/s

Data bits

Stop bits

Parity

none

Parity is not checked.

odd

Odd parity.

even

Even parity.

>RS232 settings handshake: HWs

HWs,HWf,

SWchar,SWline,none

Handshake

HWs

Reduced hardware handshake.

HWf

Full hardware handshake.

SWchar

SWline

none

Software handshake with character stop. Software handshake with line stop.

No handshake.

For detailed information on the handshake, see

5.2.8.

section

>RS232 settings character set: IBM

IBM,HP,Epson, Seiko,Citizen

Character set for printer or PC

The settings for the printers recommended by Metrohm are listed in

'Epson'

ting

section 2.7.2

. For printers not listed, the set-

is recommended. In any case the printer

handbook should be consulted. For data transfer with

'IBM'

personal computers,

must be chosen.

>RS232 settings RS control: ON

ON,OFF

Control via RS232 interface

Data receipt via RS232 interface switched

on.

OFF

Data receipt via RS232 interface switched off

(no external control via RS232 possible).

788 IC Filtration Sample Processor

4.2 Basic settings

4.2.2 Locking keyboard functions

Switch on +

CONFIG

Certain domains of the user dialog can be made inaccessible to the novice user by locking particular keys. For example, the inadvertent overwriting of a method or even the changing of parameters can be prevented in this way.

The menu

'>keyboard options'

for the corresponding functions is opened by holding down the <CONFIG> key while switching on the 788 IC Filtration Sample Processor. Alternatively, a reset can be executed by pressing <CLEAR> and then the <CONFIG> key within 0.4 seconds. This menu is also accessible when the entire keyboard has been locked

The individual key domains that can be locked are the following:

>keyboard options lock keyboard: OFF

ON,OFF

Locking the entire keyboard

If, during routine use, only one particular method is to be used, it may be desirable to block manual manipulations on the 788 IC Filtration Sample Processor. Almost all the keys on the keyboard can be locked for this purpose. The <START>, <STOP> and <CLEAR/RESET> keys however, remain operable so that it is still possible to start and stop a method. This can also be beneficial when using the 788 IC Filtration Sample Processor with a PC software (e.g. «IC Net»). For this application the keyboard may be disconnected.

>keyboard options lock configuration: OFF

ON,OFF

Locking <CONFIG> key

The basic configuration of the changer can be protected from overwriting. All settings of the configuration menu are no longer accessible at this point.

>keyboard options lock parameters: OFF

ON,OFF

Locking <PARAM> key

If user methods are generally used, it might be wise to protect the stored method parameters from alteration. The parameter menu can then be made inaccessible.

788 IC Filtration Sample Processor

4 Operation

>keyboard options >user methods

Submenu for locking method storage func-

tions

>user methods lock method recall: OFF

ON,OFF

Lock loading of methods

>user methods lock method store: OFF

ON,OFF

Lock storage of methods

>user methods lock method delete: OFF

ON,OFF

Lock deletion of methods

>keyboard options lock display: OFF

ON,OFF

Locking the display

If the 788 IC Filtration Sample Processor is to be operated exclusively by an external control software (e.g. «IC Net»), the display for manual operation can be switched off.

788 IC Filtration Sample Processor

4.3 Methods

4.3.1 Structure of a method

method

x

Number of samples to be processed

x

Process sequences (start, sample and final sequence)

x

Definition of the various instrument settings (changer settings, manual stop options)

These method parameters are accessible by pressing the <PARAM> key (see be stored, loaded and deleted (see

sequence

A order specified during automatic processing of a sample series. There are functions for controlling lift and pump and for moving the turntable (racks). External instruments (732 IC Detector, 709 IC Pump, 752 Pump Unit, etc.) can be controlled using the corresponding commands.

consists of the following parts:

section 4.3.2

). With the <USER METHOD> key, methods can

section 4.3.8

is a succession of commands that are carried out in the

Processing a sample series is accomplished in three phases. These are:

x Start sequence

Sequence of commands that is exe-

cuted once at the beginning of a series.

x Sample sequence

Sequence of commands used for each

sample.

x Final sequence

Sequence of commands that is exe-

cuted once at the end of a series.

number of samples

<START>

start sequence final sequence

The creation of sequences is done in the submenus

quence'

'>sample sequence'

cessible via the parameter menu (see

A sequence is organized in lines. When a command is entered, a new line with the corresponding command is added after the command that is displayed at the moment. The line number is visible in the display. 99 lines per sequence are possible.

sample sequence

'>final sequence'

and

section 4.3.2

'>start se-

, which are ac-

normal state

788 IC Filtration Sample Processor

In a command sequence, the commands that are on the numerical keypad as alternate functions can be used. For the most part these are the same commands that are used for manual operation (see

tion 4.4

). However, in a sequence these can exhibit different or more ex-

sec-

tensive selection possibilities.

4 Operation

4.3.2 Method parameters – <PARAM> key

The <PARAM> key combines the most important parameter settings

PARAM

parameters number of samples: rack

for the 788 IC Filtration Sample Processor. All these settings constitute a method and may be saved as such (see opens the following main menu:

section 4.3.8

). The key

Number of samples to be processed (parameter)

parameters >start sequence

parameters >sample sequence

parameters >final sequence

parameters >changer settings

parameters >manual stop

Repeated pressing of the <PARAM> key selects the submenus in turn. Use <Ð> or <Ï> to move up or down one menu item; use <HOME> or <END> to move to the first or last menu item respectively. The submenu is opened with <ENTER>, <QUIT> returns to the normal state. The following listings show all dialog items which appear under <PARAM>. The values shown in the display are the default values, the possible entry values or ranges are shown below the display.

Start sequence before sample series

Sample sequence for sample series

Final sequence after sample series

Sample changer settings

Options with manual stop

parameters number of samples: rack

1}999,rack,*

Number of samples to be processed

rack

= all sample positions of the rack

= infinite number of samples

All sample positions of an engaged rack will be processed when the instrument is on the

'rack'

setting (max. number of rack positions – number of special beakers defined). It is important that the 788 IC Filtration Sample Processor can recognize the rack. This is only possible when the rack is at the ground position. It is recommended to initialize the 788 IC Filtration Sample Processor with the <CLEAR> key or <ENDSEQ> and <ENTER> after every rack change.

788 IC Filtration Sample Processor

4.3 Methods

parameters >start sequence

parameters >sample sequence

parameters >final sequence

parameters >changer settings

>changer settings rack number 2

>changer settings lift rate 1 12 mm/s

>changer settings shift rate 20

Start sequence of a sample series

Processing sequence for each sample

Final sequence of a sample series

Settings for 788 IC Filtration Sample Processor

0}16

3}12 mm/s

3}20

The processing sequence entered here is

the start

of a sample series. This can be useful for switching

executed once at

on external devices, for example. In this submenu up to 99 command lines can be entered as a

processing sequence (see

This processing sequence is

ing of every sample

section 4.3.3

executed during the process-

of a series.

In this submenu up to 99 command lines can be entered as a processing sequence (see

This processing sequence is

section 4.3.3

executed once at the end

of a sample series. This could be the useful for switching off external devices, for example.

In this submenu up to 99 command lines can be entered as a processing sequence (see

section 4.3.3

Rack that is assigned to the method

= no particular rack

This setting can force the use of a certain rack with the method chosen. If this is not desired, the rack number

'0'

must be chosen.

Stroke speed of lift

Rack shift rate

Turning speed of the rack in angular degrees/second

788 IC Filtration Sample Processor

4 Operation

parameters >manual stop

Actions at manual stop

The following entries define the commands or signals that are transmitted via the interfaces when the <STOP> key is pressed. This enables peripheral instruments connected to be stopped automatically.

>manual stop CTL Rmt: **************

14 bit (1,0 or *)

Signal output via remote interface

The 3 lines 11, 12 and 13 are occupied for the swing head and are therefore ignored (see

>manual stop CTL RS232:

14 ASCII characters

Data to be transmitted via RS232 interface

Clear value '&PR;$S'

4.3.3 Programming of sequences

section 5.1

INSERT

DELETE

The creation of sequences is done in the submenus

quence'

'>sample sequence'

cessible via the parameter menu (see

'>final sequence'

and

section 4.3.2

'>start se-

, which are ac-

Each sequence is organized in lines. In each line, the commands that are on the numerical keypad as alternate functions can be used to enter commands (see

section 4.3.6

). After selecting a command and entering the necessary data, the entry is accepted with <ENTER>. The line number is visible in the display. 99 lines per sequence are possible.

Navigation in a sequence is accomplished as in the other menus. In addition the <INSERT> and <DELETE> keys can be used.

<INSERT> adds a new command line

above the current line

sequence. It is automatically occupied by the "NOP" command that has no function. The following lines are shifted one line downwards.

<DELETE> deletes the current line in a sequence. The following lines are shifted one line upwards.

LEARN

The " tails see

" mode is available for the easy entry of parameters (de-

section 4.3.4

in a

Furthermore the " mand line step by step (details see

788 IC Filtration Sample Processor

TRACE

" function can be used to execute every com-

section 4.3.5

4.3 Methods

4.3.4 LEARN mode

LEARN

HOLD

When editing a method, the parameters of a command are most easily determined experimentally, i.e. by manual execution, and it is for this reason that certain commands are "teachable". The LEARN function makes the manual execution of particular changer commands possible during the editing of a sequence. The resulting parameters (for example, the lift position or the status of the input lines) can be taken over in the current command line. The LEARN function can be used repetitively. When times or volumes are "learned", the repetitive values are added up.

Procedure for creating a method

x

Enter a command or select an existing command line.

x

Press the <LEARN> key.



Function is started, "LEARN" LED lights up.



Press the <LEARN> key.



Function is stopped, "LEARN" LED blinks.



With the <ENTER> key, accept the value (or re-start the LEARN function).

x

"LEARN" LED goes out, edit next command line.

The LEARN function can be used with the following commands:

Command Teachable parameter Mode of function

LIFT

PUMP

WAIT

SCN Rm

SCN RS

4.3.5 TRACE function

The "TRACE" function is a valuable aid for operating through an entire

START

sequence or method (or parts thereof) for test purposes. Every command line in a sequence can be executed directly by pressing the <START> key. Upon completion of the action the next command line is displayed.

Lift position in mm absolute Pump time in sec additive Waiting time in sec additive Status of the 8 remote lines "live" value Character sequence received "live" value

788 IC Filtration Sample Processor

Tracing can be executed immediately after entry of a sequence line or at any time after opening the parameter menu and selecting a sequence.

4 Operation

4.3.6 Commands for sequences

The following commands are programmable within a sequence. Most of them are also available in manual operation but may vary partially in their operation or exhibit a limited parameter selection (see

section 4.4

SAMPLE

>start sequence 1 SAMPLE: = 1

=,+,–; 1}999

Define current sample

position

The SAMPLE command defines the rack position for the current sample

SAMPLE = X

( example, in a sample sequence (

). This is stored as a index variable. It may be modified for

SAMPLE + X

SAMPLE – X

), in order to

control the course of a sample series during processing.

The SAMPLE command does not have to be used for simple applications. As a general rule the first sample of a series is assumed to be in rack position 1 unless specified otherwise. Therefore it is recommended not to place the special beakers in the first rack positions; place them in the highest positions instead.

Before starting a sample series, the position of the first sample can be defined with the <SAMPLE> key in manual operation, as long as this has not been defined in the method itself.

If a certain sample tube order is needed for each application of a method, the position of the first sample can be defined in the start sequence with

'SAMPLE = X'

and this setting can be saved with the corre-

sponding method.

If the SAMPLE command is not executed during a sample sequence, the SAMPLE variable will be increased by 1 after every processing of the sample sequence.

MOVE

>sample sequence 2 MOVE 1 : sample

sample,spec.1}8,1}999

The MOVE command can place the current sample tube or a special beaker below the needle position by rotating the rack. An absolute rack position can also be specified.

During method processing a MOVE command automatically sets the lift to the shift position. In the parameter menu under the turning speed can be defined specifically for each method.

If there is no sample tube in the rack position chosen, this is not recognized by the IC Sample Processor, and air is aspirated instead of sample solution. Therefore always make sure that sample tubes are placed at all rack positions defined in the processing sequence.

Position vessel /

Turn rack

'>changer settings'

788 IC Filtration Sample Processor

4.3 Methods

LIFT

>sample sequence 3 LIFT: 1 : rest mm

work,rinse,shift,

special,rest,0}125 mm

Position the lift

Raising or lowering the lift to a defined position. Work, rinse, shift and special position are rack-specifically defined in the Configuration menu under

'>rack definitions'

(see

section 4.2.1

The rest position is the zero position (0 mm) of the lift, i.e. the upper stop.

Each lift can also be precisely positioned to the millimeter. The LEARN function is also available for this purpose (see

section 4.3.4

With LIFT commands, please note that if you are using the 6.1835.020 PEEK tubing, the sample tubes may not be sealed with caps because they cannot be pierced by the PEEK tubing !

PUMP

>sample sequence 4 PUMP 1.1 : 1 s

1}999 s,ON,OFF

With the <PUMP> command the peristaltic pump of the 788 IC Filtration Sample Processor can be controlled.

Pump control

The pump can be turned off and on as desired or operated for a specific amount of time. The LEARN function is useful for determining the interval of time (see

section 4.3.4

SCAN

>sample sequence 5 SCN:Rm : Ready1

Rm,RS Ready1

End1

End2 Wait1 Wait2 Wait

 Pump1 ?  Pump2 ?

 Pump ?

8 bit (1,0 or 

In a sequence the

SCN:Rm

until the predefined bit pattern is received.

Scanning the remote

interface

= Waiting until 732/1 is ready = Waiting for EOD pulse of 732/1 = Waiting for EOD pulse of 732/2 = Waiting until remote line 3 of 732/1 is set to 1 = Waiting until remote line 3 of 732/2 is set to 1 = Waiting until remote line 3 of 732/1 and 732/2

are set to 1



= Waiting until IC Pump 709/1 is running = Waiting until IC Pump 709/2 is running = Waiting until IC Pumps 709/1 and 709/2 are

running

any 8-bit pattern

command causes method processing to stop

788 IC Filtration Sample Processor

4 Operation

Predefined bit patterns are supported which can be selected by short names (e.g.

'Ready1'

'End2'

'Ready'

signifies a static "ready" line of an external instrument.

'End'

stands for pulse signals, for example EOD (= end of determination). When scanning for pulse signals parallel scanning of several lines cannot be applied.

Setting special bit patterns allows flexible control of connected instruments.

Here the following is valid:

Example

00000001

= input line 0 is active = instrument 1 "ready"

= line inactive

= line active



= arbitrary line state

The bit pattern (= line state) can be taken over interactively with the LEARN function (see

For details about the remote interface, see

section 4.3.4

section 5.1

SCAN

>sample sequence 6 SCN:RS

Rm,RS

14 ASCII characters

In a sequence the

SCN:RS

Scanning the RS232 interface

arbitrary series of 14 characters

command causes method processing to stop until the predefined character string (up to 14 characters) is received via serial RS232 interface. The received data is compared character by character.

Be sure that the interface parameters agree with those of the instrument connected (see Configuration menu

'>RS232 settings

section 4.2.1

Any letters, numbers and special characters from the character set of the IC Filtration Sample Processor can be chosen. The asterisk

'*'

may

be used as a wildcard for an arbitrary character or character string. (If

'*'

is to be interpreted as an ASCII character,

'**'

has to be set). A wildcard may be used in any position of a character string. If the first part of character string is correctly identified, the first appearance of the

'*'

character following the asterisk

is scanned. When it is found, the

next part of the character string is compared.

This function is especially suited to instruments with Metrohm remote control language. Here the autoinfo status messages can be scanned. For example, the 732 IC Detector has the following possibilities for scanning autoinfo information:

*R"*

Ready, "Ready" state reached, e.g. at program end

*S"*

Stop, instrument manually stopped

*W"*

Instrument in wait status

*E"*

Error, error message

These status messages, however, are only transmitted if the corresponding status message has been previously switched on, for example, in the the start sequence (e.g. for the "Ready" message of the 732 IC Detector 732 with the command

'CTL:RS &Set.A.R"on"'

788 IC Filtration Sample Processor

4.3 Methods

More detailed information about the syntax can be found in the Instructions for Use of the instrument from which the status messages should be transmitted.

With the LEARN function transmitted data (= character strings) can be taken over interactively (see

section 4.3.4

CTL

CTRL

>sample sequence 7 CTL:Rm: INIT

Rm,RS INIT

INIT 732 PROG R/S 1 PROG R/S 2 PUMP R/S 1

FILL A 1 INJECT A 1

FILL B/STEP 1

INJECT B 1

ZERO 1

PUMP 752 ON PUMP 752 OFF STEP MSM 753

******0*001*** ******0*010*** ******1*000*** ******0*101***

******1*010*** ******0*011*** *******1******

*******0******

14 bit (1,0 or *)

Setting the remote lines

= initialize remote lines of 788 = initialize remote lines of 732/1 and 732/2 = run/stop time program at 732/1 = run/stop time program at 732/2 = run/stop IC Pump 709/1 = switch valve A at 733/1 to "Fill" = switch valve A at 733/1 to "Inject" = switch valve B at 733/1 to "Fill" or switch

suppressor to next position = switch valve B at 733/1 to "Inject" = trigger the autozero function at 732/1 = switch on pump at 752/753 = switch off pump at 752/753 = switch suppressor at 753 to next position = run/stop IC Pump 709/2 = switch valve A at 733/2 to "Fill" = switch valve A at 733/2 to "Inject" = switch valve B at 733/2 to "Fill" or switch

suppressor to next position = switch valve B at 733/2 to "Inject" = trigger the autozero function at 732/2 = switch on pump at 754 (with 6.2143.220 cable) = switch off pump at 754 (with 6.2143.220 cable)

arbitrary 14 bit pattern

CTL:Rm

The

command controls external instruments via the remote interface. It causes the setting of defined line states or the sending of pulses via the 14 remote output lines.

CTRL

788 IC Filtration Sample Processor

Predefined bit patterns are supported which can be selected by short names (e.g.

'INIT 732'

'ZERO 1'

Setting particular bit patterns allows flexible control of connected instruments.

Here the following is valid:

Example

***000*******1

= line inactive

= line active



= do not change line state

= Output line 0 active

= start/stop time program at 732/1

For details about the remote interface, see

>sample sequence 8 CTL:RS

Rm,RS

Clear value: &D.S"9"

14 ASCII characters

Data communication via

serial RS232 interface

= start/stop time program at 732 arbitrary string of 14 characters

section 5.1

4 Operation

Data (= character string) can be sent to instruments connected via the serial RS232 interface.

Make sure that the transmission parameters of the RS232 interface correspond to those of the connected instrument (see Configuration menu

'>RS232 settings'

section 4.2.1

Any letters, numbers and special characters can be chosen from the character set of the 788 IC Filtration Sample Processor.

This function is suitable for instruments with Metrohm remote control language. These can be controlled with so-called triggers.

&M;$G

Go, start instrument in current mode

&M;$S

Stop, stop instrument

&M;$Q

Query, request of information

The following remote control commands (for example, in a start sequence) can switch on the autoinfo status messages of the 732 IC Detector 732:

&Set.A.R"on" &Set.A.S"on" &Set.A.W"on" &Set.A.P"on" &Set.A.E"on"

"Ready" status message status message at manual stop status message at waiting state status message at switching on the instrument status message during an error condition

To be consistent, the corresponding autoinfo messages should also be switched off again in a final sequence (

..."off"

Detailed information about the syntax of the Metrohm remote control language can be found in

section 5.2

or in the instruction manual of your instrument. Please keep the syntax and conventions of the foreign instruments or computers the 788 IC Filtration Sample Processor is communicating with.

WAIT

>sample sequence 9 WAIT 1 s

0}1}9999 s

The WAIT command sets a particular waiting interval during method processing.

Waiting time

ENDSEQ

>sample sequence 10 ENDSEQ

End mark of a sequence. This ENDSEQ command can be inserted in any desired command line for test purposes. This has the effect that the sequence (start, sample, or final sequence) is processed only up to this line.

788 IC Filtration Sample Processor

End of the sequence

4.3 Methods

4.3.7 Process control

<START>

START

With manual intervention or unexpected errors, the sample series is correctly processed and closed with the final sequence. The sample sequence is executed repeatedly according to the entry under

ples'

, beginning with the sample tube that is defined as

a method is started from the normal state. If there is no

'number of sam-

'SAMPLE'

STOP

LEARN

HOLD

RESET

CLEAR

QUIT

If the sample series is interrupted with

<STOP>

, the 788 IC Filtration Sample Processor returns immediately to the normal state. Samples that have not been processed are ignored and the end sequence is not executed. If settings for such instances have been activated under

'manual stop options'

, the corresponding actions or commands are also executed via the interfaces to stop instruments that are connected or to initiate other actions.

<HOLD>

With

the processing of a method can be interrupted. The command that is active at this point is immediately interrupted as a result. <START> continues the active sequence beginning with the command immediately following the one interrupted. Any peripheral instrument connected is

<CLEAR>

interrupts a sample series at the end of a currently active

not

stopped with the <HOLD> key.

sequence (soft break). The sample currently being processed will be completed.

<QUIT>

interrupts the command currently being executed and starts

the next command line in the sequence.

788 IC Filtration Sample Processor

If errors occur during the sample series, the corresponding

sage

is displayed and must be acknowledged with

<QUIT>

error mes-

. The 788 IC Filtration Sample Processor then goes into the HOLD status (see above). After remedying the error, <START> resumes the sequence or <STOP> halts it entirely.

During the course of a method it is possible to change all entries in the menus

'>configuration'

'>parameters'

and

. These changes have an

immediate effect on the course of the method (with few exceptions, see

section 4.2.1

Care should be taken when editing process sequences. These can be edited "live" (including insertion or deletion of a command line). The TRACE and LEARN functions however, are not available in this case. Testing of the edited function is therefore not possible. Illogical command sequences could easily result, which would cause an error condition and force the interruption of a sample series.

4 Operation

4.3.8 User defined methods

USER

METHOD

fined methods, which can be stored and recalled under freely selectable names. A method comprises the parameters defined under the <PARAM> key. The key opens the following main menu:

The <USER METHOD> key is used for the management of user de-

methods >recall method

methods >store method

methods >delete method

Recall method

Store method

Delete method

Repeated pressing of the <USER METHOD> key selects the submenus in turn. Use <Ð> or <Ï> to move up or down one menu item; use <HOME> or <END> to move to the first or last menu item respectively. The submenu is opened with <ENTER>, <QUIT> returns to the normal state. The following listings show all dialog items which appear under <USER METHOD>. The values shown in the display are the default values, the possible entry values or ranges are shown below the display.

methods >recall method

>recall method method: ********

methods >store methods

>store methods method: ********

Recall existing method

8 ASCII characters

Storage of methods

8 ASCII characters

Selection of the method

With <SELECT> any method saved can be chosen. If an "empty" method is to be loaded, the method

'********'

can be selected. Like this, the actual work

memory is deleted.

Define method name

"<" or ">" activates text edit mode where any method name desired can be entered (see

section 4.1.6

When a certain method should be worked off after switching on the 788 IC Filtration Sample Processor, a command sequence can be stored under the name

'POWERUP'

switching on the main switch (see

. This method is started automatically after

section 4.3.9

788 IC Filtration Sample Processor

4.3 Methods

methods >delete method

Deletion of methods

>delete method method: ********

8 ASCII characters

Selection of the method

Selection of the method which should be deleted using the <SELECT> key.

>delete method delete ******** ?

Confirmation of the deletion

Confirmation with <ENTER> Abort with <QUIT>

4.3.9 POWERUP method

When the 788 IC Filtration Sample Processor is switched on, the sample rack and the needle are brought into the rest position. To bring them to other positions, the "POWERUP" method can be used. This method is started automatically, when the 788 IC Filtration Sample Processor is switched on.

Create a method that contains the command sequence which should be worked off, when the 788 IC Filtration Sample Processor is switched on. Store this method under the name

'POWERUP'

(see

section 4.3.8

788 IC Filtration Sample Processor

4 Operation

4.4 Manual operation

4.4.1 Turning the sample rack / Positioning the samples

Using the <Í> and <Î> keys the sample rack can be turned one position to the left (in the counterclockwise direction) or right (clockwise).

MOVE

4.4.2 Moving the lift

manual operation MOVE 1 : sample

sample,spec.1}8,

1}999

Position vessel /

Turn rack

With the MOVE command, a particular sample tube or other vessel can be placed under the needle. With <SELECT> the numerical rack position as well as the predefined current sample (SAMPLE command) or the special beakers 1-8 can be chosen.

For security reasons turning the sample rack is only possible when the lift is in or above the shift position.

The keys <Ï> and <Ð> allow upward and downward movement of the lift. The lowest possible lift position is defined by the configuration parameter

'max. lift way'

The <HOME> key runs the lift to the rest position (0 mm), i.e. to

HOME

LIFT

END

the upper limit. <END> runs the lift to the predefined work position (see

tion 4.3.2

manual operation LIFT: 1 : rest mm

Position the lift

work,rinse,shift,

special,rest,

0}125 mm

With the LIFT command, the lift can be run to a given position. In addition to selecting an exact position in mm (0 - 125 mm), the <SELECT> key can select a predefined position (work position, rinse position, shift position, special position, rest position = 0 mm).

788 IC Filtration Sample Processor

sec-

4.4 Manual operation

4.4.3 Setting the sample position

SAMPLE

manual operation SAMPLE: = 1

The <SAMPLE> command serves to set the current sample position. It defines the first sample tube for the subsequent sample series.

4.4.4

4.4.4 Pump control

4.4.44.4.4

PUMP

******** counter 1/128 PUMP+ ready

The PUMP command is used to switch on/off the peristaltic pump at the 788 IC Filtration Sample Processor. The pump command switches the selected pump on or off, according to the current state. The current state of the pumps is shown directly in the display:

Pump switched on:

1}999

PUMP+,PUMP-

PUMP + (+ = ON)

Define current sample

position

Switch pump on/off

Pump switched off:

By pressing <STOP> the pump is also stopped.

4.4.5 Display interface signals

SCAN

manual operation SCN:Rm :00000000

The

SCN:Rm

command causes the signal states of the incoming remote lines to be displayed in binary form ( inactive).

manual operation SCN:RS

Rm,RS ASCII

characters

The

SCN:RS

command causes the character string received at the RS232 interface to be displayed line by line (14 characters).

PUMP - (- = OFF)

Rm,RS

0,1

Display remote interface

signals

= line active, 0 = line

Display RS232 interface

signals

788 IC Filtration Sample Processor

4 Operation

4.4.6 Interface control

CTRL

manual operation CTL:Rm : INIT

Rm,RS INIT

INIT 732 PROG R/S 1 PROG R/S 2 PUMP R/S 1

FILL A 1 INJECT A 1

FILL B/STEP 1

INJECT B 1

ZERO 1

PUMP 752 ON PUMP 752 OFF STEP MSM 753

******0*001*** ******0*010*** ******1*000*** ******0*101***

******1*010*** ******0*011*** *******1******

*******0******

14 bit (1,0 or *)

Setting the remote lines

arbitrary 14 bit pattern

The

CTL:Rm

command controls external instruments via the remote interface. It causes the setting of defined line states or the sending of pulses via the 14 remote output lines. Predefined bit patterns are supported which can be selected by short names (e.g.

'ZERO 1'

'INIT 732'

Here the following is valid:

Example

***000*******1

= line inactive

= line active



= do not change line state

= Output line 0 active

= start/stop time program at 732/1

For details about the remote interface, see

manual operation CTL:RS

Rm,RS

Clear value: &D.S"9"

14 ASCII characters

Data communication via

RS232 interface

= start/stop time program at 732 arbitrary string of 14 characters

section 5.1

Using the

CTL:RS

command data (= character string) can be sent to

instruments connected via the serial RS232 interface.

For details about the remote interface, see

5.2

section 4.3.6

and

788 IC Filtration Sample Processor

section

4.4 Manual operation

4.4.7

4.4.7 Print out reports

4.4.74.4.7

******** counter 1/128 Print: param

config

param

usermeth

Print out a report

all

All reports in the order usermeth, config, param

Configuration report Method parameter report Report of stored methods

The <PRINT> key is used for the output of reports on an external printer or PC connected to the RS232 interface (see

section 2.5

Example of "config" report

'co 788 IC Filtr. Proc. 5.788.0010 configuration >auxiliaries dialog: english display contrast 3 beeper: ON device label program 5.788.0010 max. lift way 125 mm >rack definitions number code type 1 000110 M128-2 work position 125 mm rinse position 125 mm shift position 0 mm special position 0 mm position special beaker 1...8 127 128 0 0 0 0 0 0 number code type 2 010001 M129-2 work position 125 mm rinse position 125 mm shift position 0 mm special position 0 mm position special beaker 1...8 128 129 0 0 0 0 0 0 number code type 3 001010 M142-2 work position 125 mm rinse position 125 mm shift position 0 mm special position 0 mm position special beaker 1...8 142 0 0 0 0 0 0 0 >RS232 settings baud rate: 9600 data bit: 8 stop bit: 1 parity: none handshake: HWs character set: IBM RS control: on

------------

788 IC Filtration Sample Processor

4 Operation

Example of "param" report

'pa 788 IC Filtr. Proc. 5.788.0010 parameters method ******** number of samples: rack >start sequence >sample sequence >final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

Example of "usermeth" report

'um 788 IC Filtr. Proc. 5.788.0010 user memory >methods PC 760 PC Seg 808 SP 872 SP Seg 920 761 632 761 Seg 672 An Cat 1328 AnCatSeg 1416 Preconc 1024 Dialysis 1656 remaining bytes 17808

------------

788 IC Filtration Sample Processor

Metrohm 788IC User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Table of contents

1 Introduction

1.1 Instrument description

1.2 Parts and controls

1.3 Information about the Instructions for Use

1.3.1 Organization

1.3.2 Notation and pictograms

1.4 Support documentation

1.4.1 Application Bulletins

1.4.2 Application Notes

1.5 Safety notes

1.5.1 Electrical safety

1.5.2 General safety rules

2.1 Setting up the instrument

2 Installation

2.1.1 Packaging

2.1.2 Check

2.1.3 Location

2.1.4 Arrangement of the instruments

2.2 Mains connection

2.2.1 Setting the mains voltage

2.2.2 Fuses

2.2.3 Mains cable and mains connection

2.2.4 Switching the instrument on/off

2.3 Attaching the accessories

2.3.1 Connecting the swing head

2.3.2 Connecting the keyboard

2.3.3 Installing the plug cover

2.3.4 Installing the splash protection

2.3.5 Installing the needle or tubing

2.3.6 Placing the sample rack

2.3.7 Adjusting the sample rack

2.3.8 Assembling the ultra-filtration cell

2.3.9 Tubing connections

2.3.10 Tubing connections 788 – 754 Dialysis Unit

2.3.11 Fixing tubing and cables

2.4 Installation for full control with «IC Net»

2.4.1 Connection options

2.4.2 General information on interface connections

2.4.3 Metrohm IC systems

2.5 Installation with compact IC systems

2.5.1 Connection to 761 Compact IC or 790 Personal IC

2.6 Installation for simple remote control of the IC system

2.6.1 IC system without suppression

2.6.2 IC system with suppression with 788 as "Master"

2.6.3 IC system with suppression with PC as "Master"

2.6.4 IC system for simultaneous anion/cation determination

2.6.5 IC system with sample dialysis

2.7 Connection of devices to the RS232 interface

2.7.1 General information on RS232 interface

2.7.2 Connecting a printer

3.1 Prerequisites / Preparations

3 Operating tutorial

3.2 Configuration

3.3 Rack configuration

3.4 Methods

3.5 "Tracing"

4.1 Fundamentals of operation

4 Operation

4.1.1 Display

4.1.2 Keyboard

4.1.3 Overview of key functions

4.1.4 Instrument dialog

4.1.5 Data entry

4.1.6 Text entry

4.2 Basic settings

4.2.1 Configuration – <CONFIG> key

4.2.2 Locking keyboard functions

4.3 Methods

4.3.1 Structure of a method

4.3.2 Method parameters – <PARAM> key

4.3.3 Programming of sequences

4.3.4 LEARN mode

4.3.5 TRACE function

4.3.6 Commands for sequences

4.3.7 Process control