M(O2)Molecular weight (32000 mg/mol)
RGas constant (83.144 lmbar/molK)
T
0
Reference temperature (273.15 K)
T
m
Measuring temperature
V
t
Bottle volume (nominal volume in ml)
V
l
Sample volume in ml
aBunsen absorption coefficient (0.03103)
p(O
2
)Difference of the D. O. partial pressure (mbar)
1Overview
1.1The system OxiTop
®
Control
The classical application field for the OxiTop® measuring heads and controller
is the BODx determination (BODx = Biochemical Oxygen Demand for the time
x). The evaluation of biological degradability (e.g. test according to OECD
301F) is also part of this field.
With different sample vessels, the OxiTop
®
measuring heads and the OxiTop®
Controller 110 can also be used for other applications such as:
Evaluation of respiration and toxicity in earth, sludge, waste and sediment
(e.g. extraction of contaminated earth according to recovery concepts)
Evaluation of the respiration rate of cell cultures
Microbiological growth and stress examinations
Measurement of anaerobic degradation processes (e.g. biogas determina-
tion with the measuring head OxiTop
®
-C/B)
Accessories: see WTW catalog and price list
1.2The measurement principle
The respirometric measurement is a pressure measurement. If oxygen is
consumed in a closed vessel at a constant temperature, a negative pressure
develops. If a gas is released, an overpressure develops. The OxiTop
measuring head measures and stores this pressure for the whole duration of a
measurement once started.
®
The OxiTop
OC 100 controller collects the pressure values from the
measuring heads and processes them.
The formula shown below is the basis for all calculations for the BOD using the
®
values from the OxiTop
measuring head.
®
ba31114e0708/20147
OverviewOxiTop® Control OC 100
The interpretation of the pressure differences in the temporal course depends
on the measured material and its preparation and on the sample manipulation
(e. g. intermediate aerations) during the measuring period.
1.3Data management
The measuring head records the measured values and stores the measuring
data. Using the controller, the collected and stored data are read out from the
measuring head and stored in the “sample management“. Starting at the
OxiTop
®
sample management, it is possible to make the graphical and statis-
tical evaluation of the sample data.
The data can be transferred from the controller to a computer via the RS232
interface. The read-out software Achat OC facilitates to process the stored data
further.
Additionally, the data can be transferred to an IR printer via the IR interface.
1.4Literature
Further information and application reports on the subject of measuring with the
system OxiTop
CD-ROM Applications for OxiTop
®
Control can be obtained at no cost from WTW:
®
Control
CD-ROM Principles of measuring technique
8ba31114e0708/2014
OxiTop® Control OC 100Safety
2Safety
2.1Safety information
2.1.1Safety information in the operating manual
This operating manual provides important information on the safe operation of the instrument. Read this operating manual thoroughly and
make yourself familiar with the instrument before putting it into operation or working with it. The operating manual must b e kept in the vicinity
of the instrument so you can always find the information you need.
Important safety instructions are highlighted in this operating manual.
They are indicated by the warning symbol (triangle) in the left column.
The signal word (e.g. "CAUTION") indicates the danger level:
WARNING
indicates a possibly dangerous situation that can lead to serious (irreversible) injury or death if the safety instruction is not
followed.
CAUTION
indicates a possibly dangerous situation that can lead to slight
(reversible) injury if the safety instruction is not followed.
NOTE
indicates a possibly dangerous situation where goods might be
damaged if the actions mentioned are not taken.
2.1.2Safety signs on the meter
Note all labels, information signs and safety symbols on the instrument.
A warning symbol (triangle) without text refers to safety information in
this operating manual.
2.2Safe operation
2.2.1Authorized use
Only the operation and running of the instrument according to the
instructions and technical specifications given in this operating manual
is authorized (see section 11 T
ECHNICALDATA, page 79).
Any other use is considered unauthorized.
2.2.2Requirements for safe operation
Note the following points for safe operation:
•The instrument may only be operated according to the authorized
ba31114e0708/20149
SafetyOxiTop® Control OC 100
use specified above.
•The instrument may only be supplied with power by the energy
sources mentioned in this operating manual.
•The instrument may only be operated under the environmental
conditions mentioned in this operating manual.
•The instrument may only be opened if this is explicitly described in
this operating manual (example: Changing the batteries).
2.2.3Unauthorized use
The instrument must not be put into operation if:
•it is visibly damaged (e.g. after being transported)
•it was stored under adverse conditions for a lengthy period of time
(storing conditions, see section 11 T
ECHNICALDATA, page 79).
10ba31114e0708/2014
OxiTop® Control OC 100Commissioning
3Commissioning
3.1Scope of delivery
Controller OxiTop® OC 100
Battery holder with batteries (3 x Mignon, AA, AM3, LR6)
Operating manual
Additional scope of delivery: depending on set composition
3.2Power supply
The controller OxiTop® OC 100 and measuring head OxiTop®-C or OxiTop®C/B are both battery-powered.
Additionally, the controller has a data backup battery. This battery ensures that
your measuring data and instrument settings are stored in the controller
OxiTop
Size: Mignon, AA, AM3, LR6
Runtime: > 100 h
(approx. 1000 start-ups in normal use)
Data backup1 x lithium battery CR2430 (accessory).
Runtime: typically 4 years
Measuring
head
Power supplyBatteries: 2 x lithium battery CR2430 (accessory).
Runtime: typically 2 years
3.2.2Battery status signals
®
The OxiTop
-OC 100 controller and the OxiTop® measuring heads are powered by batteries. A low battery status of the controller or measuring head is
automatically indicated by a display message and a signal tone after the instrument is switched on (see section 10.2 D
The current battery status can also be queried in the menu:
Controller: Menu GLP/TOOLS- Check- Controller Info
Measuring head: Menu GLP/TOOLS-Check - info
ba31114e0708/201411
CommissioningOxiTop® Control OC 100
LISTED 8F93
LaboratoryEquipment
E163694
This device complies with Part 15 of the FCC Rules. Operation
is subject to the following conditions: (1)This devise may not
cause harmful interference, and (2) this devise must accept
any interference received, including interference that may
cause undesired operation.
Ser.-Nr.:05150013
OxiTop OC 1x0
WTW 82362 Weilheim
Made in Germany
1x CR 2430 Lithium
3xAlkaline, AA size, 1,5V
1
1
1
1
3.2.3Energy saving feature (automatic switch-off)
The instrument switches off automatically following the last key actuation after
expiry of the specified switch-off interval.
To specify the switch-off interval: see section 7.4.8 S
WITCH-OFFINTERVAL, page
49.
3.3Initial commissioning
Perform the following activities:
Insert the supply battery holder in the controller (see section 3 .3.1 I
THESUPPLYBATTERYHOLDER - CONTROLLER, page 12)
Switch on the instrument
Set the date and time (see section 7.4 S
ETTINGS, page 43)
3.3.1Inserting the supply battery holder - controller
Loosen the 4 screws underneath the housing using a Phillips screwdriver.
NSERTING
1Screws
Place the controller on the lower case.
Remove the upper case and put it down to the right next to the lower case
with the display downwards.
Connect the supply battery holder with the power supply contacts of the
upper case.
Insert the supply battery holder in the lower case with the open side down.
12ba31114e0708/2014
OxiTop® Control OC 100Commissioning
1
2
1 Supply batteries in the battery holder (lower case)
2 Data backup battery (upper case)
Set the upper case on top of the lower case, turn the controller and tighten
the housing screws using the screwdriver.
Switch on the controller. The battery insertion was successful if no error
message appears concerning the supply batteries.
ba31114e0708/201413
CommissioningOxiTop® Control OC 100
14ba31114e0708/2014
OxiTop® Control OC 100Operation
ON/OFF
GLP
RUN/ENTER
1
2
5
34
7
8
6
The function keys are used to start a function or to change to another
function. Confirmed data and settings are preserved.
1<On/Off>Switching on/off
2<PRINT>Printing of measurement data and set-
tings via IR interface
3<RUN/
ENTER>
Confirmation of entries
4<><>Select and change settings
Function keys:
5<ORG>Sample management: List of samples,
reading/calling up/deleting the data of individual measuring heads or samples,
shows measuring heads or samples
6<COMM>Communication with measuring heads:
Start measuring, change or show settings,
call up data
7<GRAPH> Evaluation: Graphic and numerical dis-
play of measuring data
8<GLP>GLP/Tools: Change or display settings,
display free measuring heads, display or
change settings, perform checks or maintenance
ON/OFF
RUN/ENTER
GLP
4Operation
4.1General operating principles
4.1.1Keys - controller
ba31114e0708/201415
OperationOxiTop® Control OC 100
1
4.1.2Socket field - controller
1 RS232 interface
The RS232 interface is used for communication with the PC. Usage
of the RS232 interface is exclusively intended with the WTW software
"Achat OC“ (see section 8.1 A
CHAT OC SOFTWARE, page 62).
4.2Switching on
4.2.1Switching on the controller
The current date and time appear for approx. 2
seconds (important for the allocation of sample
numbers).
If the date or time is wrong, correct them under
GLP/TOOLS.
The instrument is in the sample management
mode (operating mode BOD Routine , in the
default condition).
4.2.2Switching on the measuring heads
The measuring heads are immediately ready for operation. The controller
switches the measuring heads on and off during communication.
To avoid malfunctions:
If you use two or more controllers simultaneously make sure that
the distance between the controllers is at least 3 meters!
16ba31114e0708/2014
OxiTop® Control OC 100Operation
4.3Navigation
4.3.1Operating modes
BOD Routine:
(preselected)
Measurement of single samples for a BOD
(x = 0.5h ... 99d) at 20 °C
x
The measuring range and filling volume can be selected out of seven per-
manent ranges and the corresponding filling volumes.
The function is permanently switched on
BOD Standard: Measurement of up to 12 parallel sample processes per overall sample for
a BOD
(x = 0.5h ... 99d) at 20 °C
x
The measuring range and filling volume can be selected out of seven per-
manent ranges and the corresponding filling volumes.
Automatic sample statistics with averaging
The AutoTempfunction and the GLP mode can be switched on
ba31114e0708/201417
OperationOxiTop® Control OC 100
4.3.2Menus, functions and dialogs
Example: Pressing the <COMM> button causes the controller to change to the
SELECT ACTION menu (communication with the measuring heads).
Signal tone
Action:
Press button.The instrument displays:
This symbol in the operating manual means:
A signal tone sounds.
Description of what the
action caused and possible
further request:
SELECT ACTION menu
(communication with the measuring heads):
Preselected: - Start sample.
Select a menu item with
<><>.
The selected function or line is
displayed in inverse video.
Confirm the menu item with
<RUN/ENTER>.
The submenu is opened or the
function is carried out.
Measuring headEach measuring head that receives a command from the controller indicates
this by a short flashing signal.
®
The OxiTop
measuring head is indicated by the symbol
18ba31114e0708/2014
OxiTop® Control OC 100Operation
GLP
Time
End of measurement
Start of
measurement
AutoTemp
Measurement
Data transfer
Starting the
measuring
heads
Sample
management
Evaluation
Print
Print
4.3.3The course of the measurement
Preparation before
starting the mea-
surement
Measuring
Select operating mode
Select settings
Operating mode and settings cannot be changed for a measurement once it is started!
ba31114e0708/201419
OperationOxiTop® Control OC 100
20ba31114e0708/2014
OxiTop® Control OC 100Operating mode: BOD Standard / BOD Routine
RUN/ENTER
5Operating mode: BOD Standard / BOD Rou-
tine
The BOD Standard mode (parallel sample process for up to 12 measuring
heads per overall sample) is preset in the delivery condition of the controller.
To change to the BOD Routine operating mode (single samples): see section
7.4.1 O
The handling of controller and measuring heads is basically the same in both
operating modes. Where there are differences for the BOD Routine operating
mode they are described.
5.1Sample preparation
See application reports.
Screw the OxiTop
close them tightly.
PERATINGMODE, page 43.
®
measuring heads onto the measuring vessels and
NOTE
Never use joint grease or other lubricants for your OxiTop
®
measuring
heads. Some of these products contain solvents that can cause severe
damage to the plastic housing of the measuring head.
The sealing of the measuring vessels is perfectly adequate without
grease. However, you should always wipe off heavy contamination and
®
particles on the sealing surfaces of the rubber sleeves and OxiTop
measuring heads. WTW accepts no liability for damage due to the use of
joint grease.
5.2Starting the measurement
SELECT ACTION menu (communication with the measuring
heads):
Preselected: - Start sample.
(In BOD Routine mode appears: START).
Select the measuring range with
<><>.
The filling volume required is
given in the right hand column.
The controller stores the setting
(memory function: the last
selected measurement range is
set).
ba31114e0708/201421
Operating mode: BOD Standard / BOD RoutineOxiTop® Control OC 100
RUN/ENTER
RUN/ENTER
Confirm the selected measurement range for the sample. The
automatically assigned sample
number (YY/MM/DD and
sequential number) is given in
the header line.
Additional information:
Type of measurement, run duration, measurement range,
final date, ID number.
Temp display => the AutoTemp function (see section 7.1
GLP/TOOLS
MAINMENU, page 41) is switched on.
GLP display => the GLP function (see section 7 GLP/
TOOLS, page 41) is switched on.
Change the ID number for the additional identification of the
sample (e.g. sampling location) as follows:
Select the - I.D. number menu item with <><>
Confirm with <RUN/ENTER>.
Set the required Id number (setting range 001 ... 255) with
<><>.
Confirm with <RUN/ENTER>.
Use <PRINT>
(in BOD Routine mode only), see section 6 P
to print out the entire sample information
RINTING, page
39).
Confirm the start of measure-
ment. Contact selection:
The controller repeatedly sends the start information in the
scanner mode until feedback is successfully received from the
OxiTop
®
measuring head. After the successful start message
from the measuring head:
Displays ! started !
From this
point in time, the sample exists
within the SAMPLE STATUS TYPE list (sample manage-
ment).
Subsequently, the request to
start the next measuring head is
made automatically.
In BOD Routine operating mode there is no 1... identifier in
the display, and the
controller automatically returns to the entry menu after starting
the measuring head.
22ba31114e0708/2014
OxiTop® Control OC 100Operating mode: BOD Standard / BOD Routine
Start the next measuring head
(2).
Contact selection:
The number of the measuring
head is automatically incremented.
The controller continues to work with the contact selection, i.e.
you can now start a sequence of measuring heads (parallel
sample process) without having to press any further keys by
holding the controller to the next measuring head to be
started.
When all the measuring heads provided for this sample have
been started:
Confirm Stop start with <RUN/ENTER>.
The controller returns to the entry menu.
If no measuring head is started (e.g. because the controller
was not held to - or not close enough to - a measuring head):
The start was stopped.
Use <RUN/ENTER>- Continue
start to confirm “Continue
start” and hold the controller to a
measuring head (see above).
Return to entry level menu with
-Stop start
If an attempt is made to start a measuring head that was
already started:
Displays already used!
Use <RUN/ENTER> to confirm -
New and hold the controller
to a free measuring head (see
above).
Return to entry level menu with
-Stop start
In the delivery state, the controller automatically makes space when
Start is selected if the measured value memory is full.
To do this, it deletes the oldest finished sample (if a finished sample
is available). You can change this setting to “manual erase” (see
section 7.4.6 D
ATASTORAGE, page 47).
ba31114e0708/201423
Operating mode: BOD Standard / BOD RoutineOxiTop® Control OC 100
40 cm to 1 m
RUN/ENTER
5.3Calling up all data
This function is used to call up the data of all measuring heads, no
matter in which operating mode they were started.
To call up the data of individual measuring heads: see section 5.4
SAMPLE STATUS TYPE
LIST (SAMPLEMANAGEMENT), page 26.
SELECT ACTION menu (communication with the measuring
heads):
Preselected: - Start sample.
Select the - Call up all data menu
item with <><>
Point the controller at the measuring
heads:
Queries the data of all active
measuring heads in the scanner
mode.
The controller stores the data
obtained and updates the
SAMPLE STATUS TYPE list
(sample management).
Duration of a pass: approx. 3
seconds for 12 measuring heads
in a stirring system.
If the measuring heads do not all respond in the 1st pass of the data query,
the controller searches for the missing measuring heads in the scanner mode
for approximately a further 7 seconds. Without having to press another key,
you can continue the data queries at other locations (e.g. other stirring platforms or other thermal cabinets).
Approximately 7 seconds after the last call up was performed, the scanner
mode breaks off automatically and the following display appears:
24ba31114e0708/2014
OxiTop® Control OC 100Operating mode: BOD Standard / BOD Routine
40 cm to 1 m
RUN/ENTER
Preselected:
- Continue transfer:
A new pass is made only for
measuring heads that have not
yet responded.
Return to the entry menu with -
Stop
For information on searching for
missing measuring heads, see
section 10 W
HATTODOIF..., page
69
Point the controller at the measuring
heads:
The call up of further measuring
heads is performed in the scanner mode.
Message that all the data of all
the measuring heads has been
called up. The instrument then
returns to the entry menu.
Immediately after the complete measurement data records of a finished measurement have been called up, the corresponding measuring head is given the free status. The measuring head can be
used for a new measurement. The relevant sample in the
SAMPLE STATUS TYPE list (sample management) is marked as
finished (see section 5.4 SAMPLE STATUS TYPE
MANAGEMENT), page 26), as soon as the measurement time has
LIST (SAMPLE
expired and the data of all measuring heads of this sample have
been called up.
ba31114e0708/201425
Operating mode: BOD Standard / BOD RoutineOxiTop® Control OC 100
3
2
1
5.4SAMPLE STATUS TYPE list (sample management)
Entry into the sample management.
A list of samples appears in the display (if samples are available):
1 Sample number:
Date (JJ/MM/TT) and consecutive number
2 Temporal process of the sample:
– Status bar partly filled:
The sample is not yet ready.
– Status bar filled:
The ready and complete data set of the sample can be called up
from the measuring head.
– Status bar filled plus checkmark:
The sample is ready. The complete data set is given in the controller for evaluation.
3 BOD type and run duration.
– B20h: BOD with 20 hours run duration
– BOD5: BOD with 5 days run duration
The samples started in the BOD Routine operating mode also
appear in the SAMPLE STATUS TYPE lists (sample management)
of all other modes. Data of samples that have been started in other
operating modes do not appear in the sample management of the
BOD Routine operating mode.
Reporting order:
At the upper end of the list: finished samples (if available)
Under this: current samples
Sorting of the samples:
according to date and sequential number 01 ... 99,
from the oldest to the newest sample.
26ba31114e0708/2014
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