Metrohm 889IC User Manual
889 IC Sample Center
Manual
8.889.8001EN
Metrohm AG
CH-9101 Herisau
Switzerland
Phone +41 71 353 85 85
Fax +41 71 353 89 01
info@metrohm.com
www.metrohm.com
889 IC Sample Center
8.889.8001EN
Manual
02/2010 dm
Teachware
Metrohm AG
CH-9101 Herisau
teachware@metrohm.com
This documentation is protected by copyright. All rights reserved.
Although all the information given in this documentation has been
checked with great care, errors cannot be entirely excluded. Should you
notice any mistakes please send us your comments using the address
given above.
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Table of contents
1 Introduction 1
1.1 Instrument description ......................................................... 1
1.1.1 Model versions ........................................................................ 1
1.1.2 Instrument components ........................................................... 2
1.1.3 Intended use ........................................................................... 2
1.2 About the documentation ................................................... 2
1.2.1 Symbols and conventions ........................................................ 2
1.3 Safety instructions ................................................................ 3
1.3.1 General notes on safety ........................................................... 3
1.3.2 Electrical safety ........................................................................ 3
1.3.3 Tubing and capillary connections ............................................. 5
1.3.4 Personnel safety ...................................................................... 5
1.3.5 Flammable solvents and chemicals ........................................... 6
1.3.6 Recycling and disposal ............................................................. 6
Table of contents
2 Overview of the instrument 7
2.1 Front and rear ....................................................................... 7
2.2 Opening the device ............................................................... 8
2.3 Interior view ........................................................................ 10
2.4 Interior ................................................................................. 11
2.5 Cooling option .................................................................... 12
3 Installation 13
3.1 Setting up the instrument .................................................. 13
3.1.1 Packaging .............................................................................. 13
3.1.2 Checks .................................................................................. 13
3.1.3 Location ................................................................................ 13
3.1.4 Removing the device from the packaging .............................. 13
3.2 Connecting a computer ...................................................... 14
3.3 Connecting pump and column .......................................... 15
3.4 Tubing ................................................................................. 16
3.4.1 Tubing guide ......................................................................... 17
3.5 Outlet tubings ..................................................................... 17
4 Functioning 19
889 IC Sample Center
3.6 Rinsing the syringe ............................................................. 18
4.1 Injection modes .................................................................. 19
4.2 Syringe and buffer loop ..................................................... 20
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Table of contents
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4.3 Full loop injection ............................................................... 21
4.4 Partial loopfill injection ...................................................... 24
4.5 Pickup injection .................................................................. 27
5 Handling and maintenance 31
5.1 General ................................................................................ 31
5.2 Care ...................................................................................... 31
5.2.1 Cleaning in general ................................................................ 31
5.3 Maintenance and service ................................................... 32
5.3.1 Sample loop .......................................................................... 32
5.3.2 Replacing the sample needle ................................................. 33
5.3.3 Replacing the air needle ........................................................ 35
5.3.4 Replacing the fuses ................................................................ 36
5.4 Quality Management and validation with Metrohm ....... 37
6 Troubleshooting 38
6.1 Error list .............................................................................. 38
6.1.1 Rack unit ............................................................................... 38
6.1.2 Needle unit ............................................................................ 39
6.1.3 Syringe unit ........................................................................... 40
6.1.4 Injection valve unit ................................................................. 42
6.1.5 Cooling unit .......................................................................... 42
6.1.6 Electronics ............................................................................. 42
6.2 Analytical problems ............................................................ 43
6.2.1 Autosampler .......................................................................... 43
7 Appendix 45
7.1 Samples and sample vials .................................................. 45
7.2 I/O interface ........................................................................ 45
7.2.1 Properties of the I/O interface ................................................ 46
7.2.2 Pin assignment of the remote interface .................................. 46
8 Technical specifications 47
8.1 General ................................................................................ 47
8.2 Sampling .............................................................................. 47
8.3 Analytical characteristics ................................................... 48
8.4 Programming ...................................................................... 48
8.5 Interfaces ............................................................................. 48
8.6 Options (pre-installed) ........................................................ 48
8.7 Mains connection ............................................................... 49
8.8 Safety specifications ........................................................... 49
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IV
889 IC Sample Center
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9 Conformity and warranty 51
10 Accessories 55
Index 60
Table of contents
8.9 Electromagnetic compatibility (EMC) ................................ 49
8.10 Ambient temperature ......................................................... 50
8.11 Dimensions .......................................................................... 50
9.1 Declaration of Conformity ................................................. 51
9.2 Warranty (guarantee) ......................................................... 52
9.3 Quality Management Principles ........................................ 53
10.1 Scope of delivery 2.889.0010 ............................................ 55
10.2 Scope of delivery 2.889.0020 ............................................ 57
10.3 Optional accessories 2.889.0010 ...................................... 58
10.4 Optional accessories 2.889.0020 ...................................... 59
889 IC Sample Center
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Table of figures
Table of figures
Figure 1 889 IC Sample Center - front ............................................................. 7
Figure 2 889 IC Sample Center - rear .............................................................. 8
Figure 3 Interior view without covering ......................................................... 10
Figure 4 Interior with sampling device ........................................................... 11
Figure 5 Interior with installed cooling option. .............................................. 12
Figure 6 Unpacking the 889 IC Sample Center .............................................. 13
Figure 7 Connecting pump and column ........................................................ 16
Figure 8 Guiding the rinsing tubing ............................................................... 17
Figure 9 Tubing guide from above ................................................................ 17
Figure 10 Mounting the outlet tubing ............................................................. 17
Figure 11 Mounting the leakage tubing .......................................................... 18
Figure 12 PASA™ injection concept ................................................................ 19
Figure 13 Full loop injection ............................................................................ 21
Figure 14 Replacing the sample needle ........................................................... 33
Figure 15 Pin assignment of I/O socket and plug ............................................. 46
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VI
889 IC Sample Center
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1 Introduction
1.1 Instrument description
The 889 IC Sample Center is a robust Autosampler for high sample
throughput, optimized for the challenges of the modern analytic laboratory. Its greatest advantages are speed and small sample volumes.
Special features:
￭ Control of syringes with high resolution. This enables very high preci-
sion during injections.
￭ The control per PC software makes operation readily comprehensible.
A context-sensitive online Help function is available for every window
and every dialog.
￭ As a means of enhancing safety, no needle movements are carried out
when the door is open.
￭ The optional sample cooling guarantees consistent results.
1 Introduction
Standard micro titer plates (high or low form) or sample racks can be
used. The same plates or racks must be present in the left-hand and righthand loading drawer.
1.1.1 Model versions
2.889.0010 889 IC Sample Center
With:
￭ 15 µL injection needle
￭ 500 µL syringe
￭ 1000 µL buffer loop
￭ 100 µL sample loop
2.889.0020 889 IC Sample Center Cool
With:
￭ 15 µL injection needle
￭ 500 µL syringe
￭ 1000 µL buffer loop
￭ 100 µL sample loop
￭ Accessories for cooling the samples
889 IC Sample Center
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1.2 About the documentation
1.1.2 Instrument components
The 889 IC Sample Center has the following components:
￭ 2 sample racks
Each for 48 vials.
￭ Needle arm
With air needle and drives for horizontal and vertical positioning.
￭ Injection valve
With six connectors and two switching positions (LOAD and INJECT)
￭ Syringe module
For aspirating and transferring the samples.
￭ Cooling module (optional)
For cooling the samples.
￭ USB connector
For the connection to a PC.
￭ I/O interface
For output of an inject marker signal.
1.1.3 Intended use
The 889 IC Sample Center is designed for usage as an automation system
in analytical laboratories.
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This instrument is suitable for processing chemicals and flammable samples. The usage of the 889 IC Sample Center therefore requires that the
user has basic knowledge and experience in the handling of toxic and
caustic substances. Knowledge with respect to the application of the fire
prevention measures prescribed for laboratories is also mandatory.
1.2 About the documentation
Caution
Please read through this documentation carefully before putting the
instrument into operation. The documentation contains information
and warnings which the user must follow in order to ensure safe operation of the instrument.
1.2.1 Symbols and conventions
The following symbols and styles are used in this documentation:
Cross-reference to figure legend
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The first number refers to the figure number, the
second to the instrument part in the figure.
889 IC Sample Center
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1 Introduction
Instruction step
Carry out these steps in the sequence shown.
Warning
This symbol draws attention to a possible life hazard
or risk of injury.
Warning
This symbol draws attention to a possible hazard due
to electrical current.
Warning
This symbol draws attention to a possible hazard due
to heat or hot instrument parts.
Warning
This symbol draws attention to a possible biological
hazard.
1.3 Safety instructions
1.3.1 General notes on safety
Warning
This instrument may only be operated in accordance with the specifications in this documentation.
This instrument has left the factory in a flawless state in terms of technical
safety. To maintain this state and ensure non-hazardous operation of the
instrument, the following instructions must be observed carefully.
Caution
This symbol draws attention to a possible damage of
instruments or instrument parts.
Note
This symbol marks additional information and tips.
1.3.2 Electrical safety
The electrical safety when working with the instrument is ensured as part
of the international standard IEC 61010.
889 IC Sample Center
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1.3 Safety instructions
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Warning
Only personnel qualified by Metrohm are authorized to carry out service
work on electronic components.
Warning
Never open the housing of the instrument. The instrument could be
damaged by this. There is also a risk of serious injury if live components
are touched.
There are no parts inside the housing which can be serviced or replaced
by the user.
Mains voltage
Warning
An incorrect mains voltage can damage the instrument.
Only operate this instrument with a mains voltage specified for it (see
rear panel of the instrument).
Mains cable
Warning
Replace or repair defective or frayed insulation of the mains cable.
Protection against electrostatic charges
Warning
Electronic components are sensitive to electrostatic charges and can be
destroyed by discharges.
Always pull the mains cable out of the mains connection socket before
connecting or disconnecting electrical appliances on the rear panel of
the instrument.
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889 IC Sample Center
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Fuses
Warning
Replaced burned fuses only with new fuses of the same size and the
same type, as specified next to the fuse holder or in the accessories list
in this manual.
1.3.3 Tubing and capillary connections
Caution
Leaks in tubing and capillary connections are a safety risk. Tighten all
connections well by hand. Avoid applying excessive force to tubing
connections. Damaged tubing ends lead to leakage. Appropriate tools
can be used to loosen connections.
Check the connections regularly for leakage. If the instrument is used
mainly in unattended operation, then weekly inspections are mandatory.
1 Introduction
1.3.4 Personnel safety
Wear protective goggles and working clothes suitable for laboratory
work while operating the 889 IC Sample Center. It is also advisable to
wear gloves when caustic liquids are used or in situations where glass
vessels could break.
Personnel are not permitted to reach into the working area of the
instrument while operations are running!
A considerable risk of injury exists for the user.
Warning
Warning
889 IC Sample Center
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1.3 Safety instructions
Warning
In the event of a possible jamming of a drive, the mains plug must be
pulled out of the socket immediately. Do not attempt to free jammed
sample vessels or other parts while the instrument is switched on.
Blockages can only be cleared when the instrument is in a voltage-free
status; this action generally involves a considerable risk of injury.
1.3.5 Flammable solvents and chemicals
Warning
All relevant safety measures are to be observed when working with
flammable solvents and chemicals.
￭ Set up the instrument in a well-ventilated location.
￭ Keep all sources of flame far from the workplace.
￭ Clean up spilled liquids and solids immediately.
￭ Follow the safety instructions of the chemical manufacturer.
￭ Dispose of solvents and chemicals according to good professional
practice.
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1.3.6 Recycling and disposal
This product is covered by European Directive 2002/96/EC, WEEE – Waste
from Electrical and Electronic Equipment.
The correct disposal of your old equipment will help to prevent negative
effects on the environment and public health.
More details about the disposal of your old equipment can be obtained
from your local authorities, from waste disposal companies or from your
local dealer.
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889 IC Sample Center
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1
2
3
4
2 Overview of the instrument
2.1 Front and rear
2 Overview of the instrument
Tubing guide
1
For capillaries and tubings.
Connector for the outlet tubing
3
889 IC Sample Center
Figure 1 889 IC Sample Center - front
Door to the sample chamber
2
With handle.
Connector for the condensation and
4
leakage tubing
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2.2 Opening the device
1
2
3
4
5
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Figure 2 889 IC Sample Center - rear
USB connector
1
For the connection to a PC.
I/O connector
3
For output of an inject marker signal.
Type plate
5
Contains specifications concerning mains
voltage and serial number.
2.2 Opening the device
Warning
Mobile parts are to be found in the interior of the 889 IC Sample Center. Reaching into the interior during operation puts personnel at a serious risk of injury. Open the door only if the device is in idle mode. Due
to safety considerations, no needle movements are carried out by the
device when the door is open.
Fan
2
For the cooling unit. Do not cover!
Mains connection socket
4
With mains switch and fuse holder.
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889 IC Sample Center
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2 Overview of the instrument
Opening the door
Proceed as follows:
Grip the door handle:
1
Carefully pull out the door and press it upwards until it is in a hori-
2
zontal position.
889 IC Sample Center
Slide the door into the housing.
3
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2.3 Interior view
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Removing the covering
You can remove the covering on the housing in order to make the interior
more readily accessible:
Press the two black buttons on the sides of the housing (above)
1
simultaneously.
Carefully pull out the covering towards the front.
2
2.3 Interior view
Figure 3
Interior view without covering
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889 IC Sample Center
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2.4 Interior
2 Overview of the instrument
The following parts are to be found in the interior of the 889 IC Sample
Center:
Syringe
1
Injection valve
3
Sample chamber
5
Washing bottle
7
889 IC Sample Center
Figure 4 Interior with sampling device
Needle arm
2
Sample drip pan
4
Needle washing position
6
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2.5 Cooling option
2.5 Cooling option
Figure 5 Interior with installed cooling option.
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If the cooling option is installed, pull the covering of the cooling option off
towards the front. Now you can place the sample racks or micro titer
plates.
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889 IC Sample Center
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3 Installation
3.1 Setting up the instrument
3.1.1 Packaging
The instrument is supplied in highly protective special packaging together
with the separately packed accessories. Keep this packaging, as only this
ensures safe transportation of the instrument.
3.1.2 Checks
Immediately after receipt, check whether the shipment has arrived complete and without damage by comparing it with the delivery note.
3.1.3 Location
The instrument has been developed for operation indoors and may not be
used in explosive environments.
3 Installation
Place the instrument in a location of the laboratory suitable for operation
and free of vibrations, if possible protected from corrosive atmospheres
and contamination by chemicals.
The instrument should be protected against excessive temperature fluctuations and direct sunlight.
3.1.4 Removing the device from the packaging
Grip the device at the positions marked with arrows and lift it out of the
packaging.
Figure 6
Unpacking the 889 IC Sample Center
889 IC Sample Center
Grip the device underneath with both hands when you are transporting it.
Always hold the device in an upright position.
Before you switch on the 889 IC Sample Center, allow the device to stand
for at least one hour in order to adapt to the room temperature.
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