Page 1
Quick Guide
Kjeldahl Sampler System
K�370/371
93131
en
Page 2
Page 3
Table of contents
Table of contents
1 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.1 Proper use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.2 Improper use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.3 Instrument-related hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.4 Other hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.5 General safety measures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.6 Safety measures prior to maintenance work. . . . . . . . . . . . . . . . . . . . . . . 5
1.7 Safety measures concerning transport and shipping . . . . . . . . . . . . . . . . . . 5
2 Daily maintenance before working with the instrument . . . . . . . . . . . . . . . . . . 6
2.1 pH electrode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.2 Calibrating the pH electrode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.3 Priming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.4 Preheating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3 Working with the Autosampler K-371 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.1 Defining blanks using the express rack . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.2 Defining a sample rack and adding sample information to this rack . . . . . . . . . . . 8
3.3 Measuring blanks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.4 Blank consideration for samples in a rack . . . . . . . . . . . . . . . . . . . . . . . . 8
3.5 Measuring samples in a rack with blank consideration. . . . . . . . . . . . . . . . . . 9
4 Working without the Autosampler K-371 . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.1 Sample determination in a group with blank correction . . . . . . . . . . . . . . . . 10
4.1.1 Group definition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.1.2 Data input . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.1.3 Blank determination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4.1.4 Definition of blank consideration. . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.1.5 Sample determination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5 View and print results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 View results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 Printing results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
6 Daily maintenance after working with the instrument . . . . . . . . . . . . . . . . . . 18
6.1 Cleaning the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.2 pH electrode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
7 Declaration of conformity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Read this manual carefully before installing and running your system and note the safety precautions.
Store the manual in the immediate vicinity of the PC running the software, so that it can be consulted
at any time.
No technical modifications may be made to the system without the prior written agreement of Buchi.
Unauthorized modifications may affect the system safety or result in accidents.
This manual is copyright. Information from it may not be reproduced, distributed, or used for competitive purposes, nor made available to third parties. The manufacture of any component with the aid of
this manual without prior written agreement is also prohibited.
If you need another language version of this manual, you can download it at
www.buchi.com.
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1 Safety
This chapter points out the safety concept of the instrument and contains general rules of behavior
and warnings from hazards concerning the use of the product.
The safety of users and personnel can only be ensured if these safety instructions are strictly observed
and followed.
1.1 Proper use
The instrument has been designed and built for laboratories. It serves for nitrogen determination
according to Kjeldahl. The AutoKjeldahl Unit K-370 as stand-alone instrument may also be used for
distillations of steam-volatile substances.
In order to guarantee proper function of the transfer process, all sample particles must be smaller than
1.5 mm.
1 Safety
1.2 Improper use
Applications not mentioned above are improper. Also applications which do not comply with the technical data are considered improper.
The operator bears the sole risk for any damages caused by such improper use.
The following uses are expressly forbidden:
Use of the instrument in rooms which require ex-protected instruments.
•
Use on samples which can explode or inflame (e.g.: explosives, etc.) due to shock, friction, heat or
•
spark formation.
1.3 Instrument-related hazards
Warning
Surface temperature exceeds 60 °C.
WARNING
Risk of injury.
Never operate the instrument when glassware is damaged.
•
1.4 Other hazards
Fundamental dangers arise from
hot acids and lye
•
flammable gases or solvent fumes in the direct vicinity of the instrument
•
damaged glass parts
•
insufficient distance between the instrument and the wall
•
burns caused by contact with hot glass parts
•
burns caused by contact with steam at the waste-outlet
•
defective transfer tube: escape of steam and/or sulfuric acid
•
4
K-370/371 Quick Guide, Version B
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1.5 General safety measures
Always wear personal protective equipment such as protective eye goggles, protective clothing and
gloves when working with the instrument.
1.6 Safety measures prior to maintenance work
WARNING
Electrical hazard:
•
Prior to all maintenance work on the instrument switch off the power supply and remove all
sources of flammable vapor.
WARNING
All maintenance and repair work requiring the opening or removal of instrument covers may only be
carried out by trained personnel and with the tools provided for this purpose.
1 Safety
WARNING
Hot surface. The steam generator heats up during operation.
Always let the instrument cool down after operation before opening the service door.
•
1.7 Safety measures concerning transport and shipping
WARNING
At least two people must be involved in the manual transport of the Autosampler K-371 due to its
heavy weight of 42 kg. Watch your fingers when putting the instrument down.
WARNING
Biohazard:
Remove all dangerous substances from the instrument and clean it thoroughly.
•
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2 Daily maintenance before working with the instrument
2 Daily maintenance before working with the instrument
2.1 pH electrode
Rinse the pH electrode and place it into the receiving vessel.
2.2 Calibrating the pH electrode
NOTE
It is recommended to use buffer solutions pH 4.00 and 7.00.
Discard buffer solutions after usage. Work with fresh solutions every day.
Select Operator > pH Calibration. Press
[Run], and follow the SOP given by the software.
2.3 Priming
Priming is used to prepare the entire system. Prior to the priming procedure check the chemical level
and switch on the cooling water. Priming includes distillation and titration with a clean and empty
sample tube. It is recommended to perform a priming at least once a day, before starting analysis. The
priming method can be modified.
Prime the system as follows:
Select Operator > System Preparation >
Priming.
Activate Proceed via Sampler if an Autosampler
K-371 is connected.
Prime the system 1 - 3 times by pressing [Run]
before starting the analysis.
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2.4 Preheating
The glass parts of the distillation system have to be preheated prior to analysis (holding time > 10 min).
This is done by means of a clean and empty sample tube. It is recommended to perform a preheating,
when the glass (splash protector) has cooled down. The preheating time is predefined.
2 Daily maintenance before working with the instrument
Select Operator > System Preparation >
Preheating.
Activate Proceed via Sampler by pressing the
Enter key if an Autosampler K-371 is connected.
Start the preheating process by pressing [Run].
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3 Working with the Autosampler K-371
The methods described in the following just show one of various possibilities. Other procedures are
described in the Operation Manual.
3.1 Defining blanks using the express rack
Select Configurator > Rack and press [New]. Select the Group and User to which you want to
assign the new Rack (for Group definition see chapter 4.1.1).
Press [Page] twice and enter a name for the rack with the blanks.
Select Express by pressing the Tab key
Press the Enter key .
Press the Tab key again.
Press the Enter key .
Enter the information for Blank 1 and press [Save] and then [Next].
Enter the information for Blank 2 and press [Save] and then [Next].
Enter the information for Blank 3 and press [Save].
Press [Close].
twice.
3 Working with the Autosampler K-371
3.2 Defining a sample rack and adding sample information to this rack
Select Configurator > Rack and press [New]. Select the Group and User to which you want to
assign the new Rack.
Press [Page] twice and enter a rack name.
Press [Save]. Press the Tab key and then press the Enter key .
Enter the sample information for Sample 1, then press [Save].
Press [Next] and enter the sample information for Sample 2.
Continue until you have entered all samples.
Press [Save], then press [Close].
3.3 Measuring blanks
Place the blanks for the measurement into the Express rack.
Select Operator > Determination > Rack with sampler and search for the rack you have defined
with the blank samples.
Press the Enter key .
Press [Run] to start the measurement of the blanks.
3.4 Blank consideration for samples in a rack
Select Operator > Determination > Rack with sampler. Search for the Rack with the samples
for which you like to use the blanks and press the Enter key , then press [Blank]. Select [Assign
blanks manually], press the Enter key .
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3 Working with the Autosampler K-371
Select the blank to be considered and press
[Set].
Select the sample(s) with the Tab key and activate it/them by pressing the Enter key .
Press [Set] and then 4 times [Close].
3.5 Measuring samples in a rack with blank consideration
Place the samples for the measurement into the 20 rack.
Select Operator > Determination > Rack with sampler and search for the rack you have defined
with your samples. Press the Enter key .
Press [Run] to start the measurement of the samples.
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4 Working without the Autosampler K-371
4 Working without the Autosampler K-371
4.1 Sample determination in a group with blank correction
4.1.1 Group definition
Use the Group Default or define a new Group.
Select Configurator > Group and press [New].
Enter a Group Name (e. g. Meat 1006).
Press [Save] then press [Close].
4.1.2 Data input
Select Configurator > Group.
Select the group e. g. Meat 1006 and open it with
Enter .
Open a new sample by pressing [New].
Fill in the sample information.
E. g.:
Name = Blank 20
Type = Blank
Method = Standard
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4 Working without the Autosampler K-371
Press [Next] to enter information for the next
sample.
Press [Yes] to save the information for Blank 20.
Fill in the sample information.
E. g.:
Name = Blank 21
Press [New] to enter information for the next
sample.
Press [Yes] to save the information for Blank 21.
Fill in the sample information.
E. g.:
Name = Sample 20
Weight = 2.879
Type = Sample
Protein Factor = 6.25
Press [New] to enter the information for the next
sample.
Press [Yes] to save the information for Sample
20.
Fill in the sample information.
E. g.:
Name = Sample 21
Weight = 2.5910
Press [Save] to save the information for Sample
21.
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4.1.3 Blank determination
NOTE
This step only needs to be carried out when sample definition in a group should be performed with
blank correction.
4 Working without the Autosampler K-371
Press [Close] three times.
Select Operator > Determination and press
the Enter key .
Select Group and press then Enter key .
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4 Working without the Autosampler K-371
Select the Group Meat 1006 and press the Enter
key to open the Group.
Place the tube with Blank 20 into the instrument.
Press [Run] to start Blank 20.
The display switches to the Status mode and
shows the different steps of the determination
method. At the end of the determination, State
will show Ready.
Press [Page] to see the Result of Blank 20.
Place the next tube (Blank 21) into the instrument.
Press the Mode key to switch to the Operator.
Select the next sample (Blank 21) and start the
determination by pressing [Run].
The display switches to the Status mode and
shows the different steps of the determination
method. At the end of the determination, State
will show Ready.
Press [Page] to see the Result of Blank 21.
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4.1.4 Definition of blank consideration
NOTE
This step only needs to be carried out when sample definition in a group should be performed with
blank correction.
4 Working without the Autosampler K-371
The mean value of the blank determinations,
Blank 20 and Blank 21, is set to be considered
for the following determination of samples.
Select Operator > Determination > Group >
Group Name (e. g. Meat 1006) and press the
Enter key .
Press [Blank].
Select the first blank value (e. g. Blank 20)
and press [First].
Select the last blank value (e. g. Blank 21)
and press [Last].
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4 Working without the Autosampler K-371
Press [Calc] to define the calculation of the mean
value.
Enter the definition for the calculation of the mean
value.
E. g.:
Name = Mean Blank 20/21
Group = Meat 1006
Lower mean limit = 0.0010 ml
Upper mean limit = 0.6000 ml
Std. deviation limit = 0.050 ml
Press [Calc] to calculate.
The mean value is calculated.
Select the calculated mean value (Mean Blank
20/21) and activate it with [Set].
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4.1.5 Sample determination
4 Working without the Autosampler K-371
Select Operator > Determination > Group >
Group Name (e. g. Meat 1006) and press the
Enter key .
Place the next tube (Sample 20) into the instrument.
Select the next sample (Sample 20) and start the
determination by pressing [Run].
The display switches to the Status mode and
shows the different steps of the determination
method. At the end of the determination, State will
show Ready.
Press [Page] to see the Result of Sample 20.
Or select Configurator > Data Manager > Re-
sult > Group > Group Name (e. g. Meat 1006)
and press the Enter key .
Place the next tube (Sample 21) into the instrument.
Press the Mode key to switch to the Operator.
Select the next sample (Sample 21) and start the
determination by pressing [Run].
Press [Page] to see the result of Sample 21.
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5 View and print results
5.1 View results
5 View and print results
All results of the group are visible under:
Configurator > Data Manager > Result >
Group > Group Name (e. g. Meat 1006).
Press the Enter key to see details of the
determination (e. g. Sample 20).
Press [Blank] to see the considered blank value.
NOTE
The defined blank value is considered.
5.2 Printing results
Select Configurator > Data Manager > Result > Group. Press [Print] to print out all results
summarized in the selected group.
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6 Daily maintenance after working with the instrument
6 Daily maintenance after working with the instrument
6.1 Cleaning the instrument
At the end of the day, the system should be rinsed thoroughly by carrying out a cleaning. The splash
protector and the condenser are rinsed with water to remove sodium hydroxide residues. With regular
cleaning, the lifetime of the glass parts can be extended.
Select Operator > System preparation > Cleaning and press [Run] to start the cleaning process.
6.2 pH electrode
Rinse the pH electrode and store it in 3 mol/l KCl solution.
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7 Declaration of conformity
We BÜCHI Labortechnik AG
do hereby declare under our sole responsibility that the products:
AutoKjeldahl Unit K-370 and
Kjeldahl Sampler K-371
to which this declaration relates is in conformity with the following standards:
EN 61010-1:2002 German Version (~IEC 61010-1; VDE 0411)
Safety requirements for electrical equipment for measurements, control, and laboratory use:
General requirements.
EN 61010-2-081:2002 German Version (~IEC 61010-2-081; VDE 0411)
7 Declaration of conformity
Safety requirements for electrical equipment for measurement, control and laboratory use:
Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other
purposes.
EN 61326:2002 German Version (~IEC 61326; VDE 0843)
Electrical equipment for measurement, control and laboratory use - EMC requirements.
In accordance with the regulations of EU guidelines:
73/23/EEC (Electrical special tools/low voltage guidelines)
89/336/EEC (electromagnetic compability)
Flawil, July 01, 2003
BÜCHI Labortechnik AG
Meierseggstrasse 40
Postfach
CH-9230 Flawil 1
Switzerland
Tel.: +41 (0)71 394 63 63 Guido Worch
Fax: +41 (0)71 394 65 65 Quality Manager
buchi@buchi.com
www.buchi.com
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BÜCHI Labortechnik AG
CH-9230
T +41 71 394 63 63
F +41 71 394 65 65 093131
Flawil 1 / Switzerland
www.buchi.com Quality in your hands
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