BUCHI C-660 User Manual

Operation Manual

Fraction Collector

C 660

096969 en

Table of contents

Table of contents

1	About this manual . . . . . . . . . . . . . . . . . .		. . . . . . . . . . . . . . . . . .	. .		. 5
	1.1	Reference documents				5
	1.2	Trademarks . . . . . . . . . . . .	. . . . . . . . . . . .	.	.	5
	1.3	Abbreviations . . . . . . . . . . . .	. . . . . . . . . . . .	.		5
2	Safety . . . . . . . . . . . . . . . . . . . . . . . . .		. . . . . . . . . . . . . . . . . .	. .		. 6
	2.1	User qualification . . . . . . . . . .	. . . . . . . . . . . .	.	.	6
	2.2	Proper use . . . . . . . . . . . .	. . . . . . . . . . . .	.	.	6
	2.3	Improper use . . . . . . . . . . . .	. . . . . . . . . . . .	.		6
	2.4	Warning notices used in this manual . . . .	. . . . . . . . . . . .	.		7
	2.5	Product safety				7
	2.5.1	Instrument-related hazards . . . . . . .	. . . . . . . . . . . .	.	. 7
	2.5.2	Other hazards				8
	2.5.3	Safety measures . . . . . . . . . .	. . . . . . . . . . . .	.	.	8
	2.6	General safety rules . . . . . . . . . .	. . . . . . . . . . . .	.		8
3	Technical data . . . . . . . . . . . . . . .		. . . . . . . . . . . . . .	.	.	9. . . .
	3.1	Scope of delivery . . . . . . . . . .	. . . . . . . . . . . .	.	.	9
	3.1.1	Standard instrument				9
	3.1.2	Standard accessories . . . . . . . . .	. . . . . . . . . . . .	.	. 9
	3.1.3	Optional accessories . . . . . . . . .	. . . . . . . . . . . .	.	10
	3.2	Materials used			12
	3.3	Technical data overview			12
4	Description of function . . . . . . . . . . .		. . . . . . . . . . . . . .	.	. 14. . . . .
	4.1	Overview over the instrument . . . . . .	. . . . . . . . . . . .	.	. 14
	4.2	Function prinicple . . . . . . . . . .	. . . . . . . . . . . .	.	. 14
5	Putting into operation . . . . . . . . . . . . . . . .		. . . . . . . . . . . . . . . . . .	. .	15
	5.1	Installation site			15
	5.2	Electrical connections			15
	5.3	Installation			16
	5.3.1	Power connection			16
	5.3.2	Fitting and ferrule . . . . . . . . . .	. . . . . . . . . . . .	.	. 16
	5.3.3	Optional waste diverter valve . . . . . .	. . . . . . . . . . . .	.	. 16

Read this manual carefully before installing and running your system and note the safety precautions in chapter 2 in particular. Store the manual in the immediate vicinity of the instrument, so that it can be consulted at any time.

No technical modifications may be made to the instrument without the prior written agreement of BUCHI. Unauthorized modifications may affect the system safety or result in accidents.

This manual is copyright. Information from it may not be reproduced, distributed, or used for competitive purposes, nor made available to third parties. The manufacture of any component with the aid of this manual without prior written agreement is also prohibited.

The English manual is the original language version and serves as basis for all translations into other languages.. Other language versions can be downloaded at www..buchi..com..

3	C-660 Operation Manual, Version F

Table of contents

6	Operation . . . . . . . . . . . . . . . . . . .		. .	. . . . . . .	. .	.	.	18. . . . .
	6.1	Control elements . . . . . . . . . . . .	. .	. . . . . .	. .	.	. 18
	6.2	Display . . . . . . . . . . . . . . . .	. .	. . . . . .	. .	.		19
	6.3	Operating the instrument . . . . . . . . . .	. .	. . . . . .	. .	.		20
	6.3.1	Remote Control . . . . . . . . . . . . .	. .	. . . . . .	. .	.		20
	6.3.2	Manual control / manual mode . . . . . . . .	. .	. . . . . .	. .	.		21
	6.3.3	Setting the height of the collector arm						24
	6.3.4	Interface RS 232 . . . . . . . . . . . .	. .	. . . . . .	. .	.	. 25
	6.3.5	Peak detection . . . . . . . . . . . . .	. .	. . . . . .	. .	.		25
7	Maintenance . . . . . . . . . . . . . . . . . . . . . . . .		. . .	. . . . . . . . .	. . . . .			28
	7.1	Cleaning						28
8	Troubleshooting . . . . . . . . . . . . . . . . .		. .	. . . . . . .	. .	.		. 29. . . . .
	8.1	Malfunctions and their remedy						29
	8.2	Customer service . . . . . . . . . . . .	. .	. . . . . .	. .	.	. 30
9	Shutdown, storage, transport and disposal . . . . . . .		. . .	. . . . . . . . .	. . . . .			31
	9.1	Storage and transport						31
	9.2	Disposal						31
10	Spare parts . . . . . . . . . . . . . . . . . . . . . . . . .		. . .	. . . . . . . . .	. . . . . 32
11	Declarations and requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
	11.1	FCC requirements (for USA and Canada) . . . .	. .	. . . . . .	. .	.	. 33
	11.2	Declaration of conformity . . . . . . . . . .	. .	. . . . . .	. .	.		34

4	C-660 Operation Manual, Version F

1  About this manual

1 About this manual

This manual describes the Fraction Collector C-660 and provides all information required for its safe operation and to maintain it in good working order.

It is addressed in particular to laboratory personnel and operators.

NOTE

The symbols pertaining to safety (WARNINGS and ATTENTIONS) are explained in chapter 2.

1..1 Reference documents

•Installation Guide SepacoreControl

•Pump Modules C-601 & C-605, Pump Controller C-610 / Pump Manager C-615, Operation Manual numbers 96961 – 96965

•UV-Monitor C-630, Operation Manual numbers 96950 – 96953 and 96966

•UV-Photometer C-635, Operation Manual numbers 96957 – 96960 and 96967

•Cartridger C-670, Operation Manual numbers 96945 – 96949

1..2 Trademarks

The following product names and any registered and unregistered trademarks mentioned in this manual are used for identification purposes only and remain the exclusive property of their respective owners:

•Sepacore® is a registered trademark of BÜCHI Labortechnik AG

•Syncore® is a registered trademark of BÜCHI Labortechnik AG

•Cartridger® is a registered trademark of BÜCHI Labortechnik AG

1..3 Abbreviations

ETFE: Ethylene/Tetrafluoroethylene Copolymer

FEP: Fluorinated Ethylene Propylene

POM: Polyoxymethylene

PUR: Polyurethane

5	C-660 Operation Manual, Version F

2  Safety

2 Safety

This chapter highlights the safety concept of the fraction collector and contains general rules of behavior and warnings from hazards concerning the use of the product.

The safety of users and personnel can only be ensured if these safety instructions and the safetyrelated warnings in the individual chapters are strictly observed and followed, therefore, the manual must always be available to all persons performing the tasks described herein.

2..1 User qualification

The instrument may only be used by laboratory personnel or other persons who on account of training or professional experience have an overview of the dangers which can develop when operating the instrument.

Personnel without this training or persons who are currently being trained require careful supervision. The present Operation Manual serves as a basis for training.

2..2 Proper use

The Fraction Collector C-660 has been conceived of and constructed as a laboratory instrument. Its correct usage is the optimal and reliable collection of individual aqueous and/or organic fractions in predetermined or customized racks (e.g. chromatography or Syncore racks).

Correct usage of the Fraction Collector C-660 also includes its maintenance and careful handling of the instrument on the basis of the specifications given in the Operation Manual.

2..3 Improper use

Applications beyond those described above are improper. Furthermore, applications which do not comply with the technical data are also considered improper. The operator bears the sole risk for any damages caused by such improper use.

The following applications are expressly forbidden:

•Use of the instrument in rooms which require ex-protected instruments.

•Collection of samples which can explode or inflame due to shock, friction, heat or spark formation or that are combustible (e.g. explosive substances, etc.)

•Use in human medicine diagnostics.

•Preparation of food.

6	C-660 Operation Manual, Version F

2  Safety

2..4 Warning notices used in this manual

WARNING

Generally, the triangular warning symbol indicates the possibility of personal injury or even loss of life if the instructions are not followed.

WARNING

Electrical hazard.

WARNING

Harmful to life forms.

WARNING

Inhalation of harmful substances.

ATTENTION

With the “Read this” symbol, ATTENTION indicates the possibility of equipment damage, malfunctions or incorrect process results if the instructions are not followed.

NOTE

Useful tips for the optimum operation of the instrument.

2..5 Product safety

The Pump System is designed and built in accordance with current state-of-the-art technology, however, risks to users, property, and the environment can arise when the instrument is used carelessly or improperly.

The manufacturer has determined residual dangers emanating from the instrument

•if the instrument is operated by insufficiently trained personnel.

•if the instrument is not operated according to its proper use.

Appropriate warnings in this manual serve to make the user alert to these residual dangers.

2..5..1 Instrument-related hazards

Pay attention to the following safety notices:

WARNING

Potential risk in case of damaged glassware.

•Beware of shivering glass parts.

•Beware of defective FEP tubing.

•Beware of leaky connections and insufficiently long connection tubings.

•Beware of defective valves.

•Beware of a false positioning of the collector arm

7	C-660 Operation Manual, Version F

2  Safety

WARNING

Potential risk if solvent vapors accumulate within the instrument housing.

•Beware of damaged or cracked glass parts.

•Beware of dangerous, flammable or explosive samples.

•Operate the instrument in a fume hood or in an adequately ventilated work area.

2..5..2 Other hazards

WARNING

Certain solvents within or in the vicinity of the Pump Systems can form peroxides and/or are highly inflammable.

•Always be aware of the explosion risk when working with hazardous substances or with substances of unknown composition.

•Always use the instrument in an adequately ventilated work area.

2..5..3 Safety measures

Always wear personal protective equipment such as protective goggles, protective clothing and gloves when working with the instrument.

2..6 General safety rules

Responsibility of the operator

The head of laboratory is responsible for training his personnel.

The operator shall inform the manufacturer without delay of any safety-related incidents which might occur during the operation of the instrument. Legal regulations, such as local, state and federal laws applying to the instrument must be strictly followed.

Duty of maintenance and care

The operator is responsible for ensuring that the instrument is only operated in proper manner and that maintenance, service, and repairs are performed with care, on schedule and by authorized personnel only.

Spare parts to be used

Use only recommended consumables and spare parts for maintenance to ensure continued optimum system performance and reliability. Any modifications to the spare parts used are only allowed with the prior written permission of the manufacturer.

Modifications

Modifications to the instrument are only permitted after prior consultation with and written approval obtained from the manufacturer. Modifications and upgrades should only be carried out by an authorized BUCHI technical engineer. The manufacturer reserves the right to decline any claim resulting from unauthorized modifications.

8	C-660 Operation Manual, Version F

3  Technical data

3 Technical data

This chapter introduces the reader to the fraction collector and its main components. It contains technical data, requirements and performance data.

3..1 Scope of delivery

Check the scope of delivery according to the order number.

NOTE

For detailed information on the listed products, see www.buchi.com or contact your local dealer.

3..1..1 Standard instrument

3..1..2 Standard accessories

4

1

2

3

Table 3-1: Standard instrument

Product	Order number
Fraction Collector C-660	44900

Table 3-2: Standard accessories

Product	Order number
a FEP tubing, 1/8”×1/16”, 5 m, green	44354
FEP tubing thin, 1/16”×1/32”, 5 m,
gray	44357
b 25 fittings, 1/8”, green	40956
25 fittings, 1/16”, gray	44816
c 25 ferrules, 1/8” green	40961
25 ferrules, 1/16” gray	44269
d Tubing guide	20780

9	C-660 Operation Manual, Version F

3  Technical data

Table 3-2: Standard accessories (cont.)

		Product	Order number
		Regional power cord, 1.5 m:
		Type CH	10010
		Type Schuko (D,F)	10016
		Type GB	17835
		Type USA	10020
		Type AUS	17836
		Operation Manual:
		German	96968
		English	96969
		Spanish	96970
		French	96971
		Italian	96972
3..1..3	Optional accessories
		Table 3-3: Optional accessories
		Product	Order number
		Waste valve, valve for the Fraction	51114
		Collector. Prevents dripping during the
		fraction change.

Tubing fitting set for connecting the	44021
waste valve.

Waste level sensor, optimal accessory for 45288 the Fraction Collector. Mounted at the top

of the waste bottle the float type switch prevents the running over of the waste reservoir. Connector for GL 45 glass threads.

10	C-660 Operation Manual, Version F

3  Technical data

Table 3-3: Optional accessories (cont.)

		Product	Order number
		e 4 racks FC 12, set of 4 racks FC 12	44957
		– each for 12 tubes at 250 mL with a
	9	diameter of 40 mm (without tubes).
		f 4 racks FC 30, set of 4 racks FC 30	44958
8		– each for 30 tubes at 50 mL with
	10	diameter of 26 mm (without tubes).
	6
		g 4 racks FC 60, Set of 4 racks FC 60	44959
5		– each for 60 tubes at 20 mL with
	7	diameter of 17 mm (without tubes).
		h 10×250 mL tubes for rack FC 12,	44960
		diameter of 39 mm; height of 300 mm
		i 20×50 mL tubes for rack FC 30,	44961
		diameter of 25 mm; height of 185 mm
		j 100×20 mL tubes for rack FC 60,	44962
		diameter of 16 mm; height of 160 mm
		Chromatography table	54290
		Pivoting table for the integration of
		two pump modules, detectors, fraction
11		collector including support rod holder.

Chromatography table with monitor

54292

		holder
	12	Pivoting table for the compact integra-
		tion of the Control Unit C-620, two pump
		modules, detectors, fraction collector and
		PC including monitor.
		Easy access and safe working due to
		clearly visible tubing guides. PC and
		monitor are not included.

11	C-660 Operation Manual, Version F