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Lyovapor™ L-200/L-200 Pro
Operation Manual
11593861 | F en
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Imprint
Product Identification:
Operation Manual (Original) Lyovapor™ L-200/L-200 Pro
11593861
Publication date: 02.2020
BÜCHI Labortechnik AG
Meierseggstrasse 40
Postfach
CH-9230 Flawil 1
E-Mail: quality@buchi.com
BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experience, especially with respect to structure, illustrations and technical details.
This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for competitive purposes, nor made available to third parties. The manufacture of any component with the aid of
this manual without prior written agreement is also prohibited.
Page 3
BÜCHI Labortechnik AG Table of contents
Contents
1 About this document........................................................................................................... 7
1.1 Connected devices .............................................................................................................................. 7
1.2 Warning notices in this document........................................................................................................ 7
1.3 Symbols............................................................................................................................................... 7
1.3.1 Warning symbols .....................................................................................................................7
1.3.2 Mandatory directive symbols ...................................................................................................8
1.3.3 Mark-ups and symbols.............................................................................................................8
1.4 Trademarks.......................................................................................................................................... 8
2 Safety....................................................................................................................................9
2.1 Proper use ........................................................................................................................................... 9
2.2 Use other than that intended ............................................................................................................... 9
2.3 Staff qualification ................................................................................................................................. 9
2.4 Location of warning signs on the product .......................................................................................... 10
2.5 Residual risks .................................................................................................................................... 10
2.5.1 Faults during operation .......................................................................................................... 10
2.5.2 Damage to the ice condenser................................................................................................11
2.5.3 Glass and acrylic breakage....................................................................................................11
2.5.4 Low internal pressure.............................................................................................................11
2.5.5 Cold and hot surfaces............................................................................................................11
2.6 Personal protective equipment .......................................................................................................... 11
2.7 Modifications...................................................................................................................................... 11
3 Product description...........................................................................................................12
3.1 Description of function ....................................................................................................................... 12
3.1.1 Freezing phase ...................................................................................................................... 12
3.1.2 Main drying phase..................................................................................................................12
3.1.3 Secondary-drying phase........................................................................................................13
3.2 Configuration ..................................................................................................................................... 14
3.2.1 Front view ..............................................................................................................................14
3.2.2 Rear view ...............................................................................................................................14
3.2.3 Connections on the rear side.................................................................................................15
3.2.4 Control panel..........................................................................................................................15
3.2.5 Pro control panel....................................................................................................................16
3.3 Type plate.......................................................................................................................................... 16
3.4 Scope of delivery ............................................................................................................................... 17
3.5 Refrigerant Specification.................................................................................................................... 17
3.6 Technical data ................................................................................................................................... 17
3.6.1 Lyovapor™ L-200 ..................................................................................................................17
3.6.2 Ambient conditions.................................................................................................................18
3.6.3 Materials ................................................................................................................................18
4 Transport and storage ......................................................................................................19
4.1 Transport ........................................................................................................................................... 19
4.2 Storage .............................................................................................................................................. 19
4.3 Lifting the instrument ......................................................................................................................... 19
Operation Manual Lyovapor™ L-200/L-200 Pro iii
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Table of contents BÜCHI Labortechnik AG
5 Installation..........................................................................................................................21
5.1 Installation site................................................................................................................................... 21
5.2 Securing against earthquakes ........................................................................................................... 21
5.3 Putting the instrument in operation.................................................................................................... 22
5.3.1 Preparing the instrument........................................................................................................22
5.3.2 Establishing electrical connections ........................................................................................ 22
5.3.3 Fitting the advanced vacuum control unit ..............................................................................23
5.3.4 Manual switch on the main valve...........................................................................................24
5.3.5 Connecting the inert gas (optional feature)............................................................................25
5.3.6 Assembling pressure sensor PPG010 (optional accessory)..................................................26
5.3.7 Assembling the alternative pressure sensor (optional accessory).........................................26
5.3.8 Fitting the drain valve strainer................................................................................................27
5.3.9 Preparing the condensate drain hose....................................................................................27
5.4 Commissioning the vacuum pump..................................................................................................... 28
5.5 Establishing LAN connection............................................................................................................. 29
5.5.1 Requirements for local network settings................................................................................29
5.5.2 Preparing the instrument for app using..................................................................................30
5.5.3 Enabling BUCHI Cloud access .............................................................................................. 30
5.6 Insert SD card (Pro control panel only).............................................................................................. 30
6 Operating the control panel.............................................................................................. 32
6.1 Layout of the control panel ................................................................................................................ 32
6.2 Function bar....................................................................................................................................... 32
6.3 Menu bar............................................................................................................................................ 33
6.3.1 Start menu .............................................................................................................................34
6.3.2 Favourites menu .................................................................................................................... 34
6.3.3 Configuration menu................................................................................................................34
6.3.4 Messages menu.....................................................................................................................37
6.4 Status bar .......................................................................................................................................... 37
6.5 Carrying out freeze-drying ................................................................................................................. 39
6.5.1 Preparing the instrument........................................................................................................39
6.5.2 Starting freeze-drying.............................................................................................................39
6.5.3 Editing parameters while the process is running ...................................................................40
6.5.4 Ending freeze-drying..............................................................................................................40
6.5.5 Shutting down the instrument ................................................................................................40
6.5.6 Switching off the instrument...................................................................................................41
iv Operation Manual Lyovapor™ L-200/L-200 Pro
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BÜCHI Labortechnik AG Table of contents
7 Operating Pro control panel .............................................................................................42
7.1 Layout of Pro control panel................................................................................................................ 42
7.2 Function bar....................................................................................................................................... 42
7.3 Other symbols on the control panel................................................................................................... 44
7.4 Menu bar............................................................................................................................................ 44
7.4.1 Start menu .............................................................................................................................44
7.4.2 Favourites menu .................................................................................................................... 45
7.4.3 Method menu.........................................................................................................................45
7.4.4 Configuration menu................................................................................................................45
7.4.5 Messages menu.....................................................................................................................48
7.5 Status bar .......................................................................................................................................... 49
7.6 Editing a method................................................................................................................................ 50
7.6.1 Creating a new method..........................................................................................................50
7.6.2 Changing the name of a method............................................................................................51
7.6.3 Setting the sample collapse temperature...............................................................................51
7.6.4 Setting the gas type ............................................................................................................... 52
7.6.5 Setting the shelf loading temperature .................................................................................... 52
7.6.6 Setting the steps of a method ................................................................................................ 52
7.6.7 Setting the phases of a method ............................................................................................. 54
7.7 Deleting a method.............................................................................................................................. 56
7.8 Setting end point definitions............................................................................................................... 56
7.8.1 Connect the sensors for pressure difference test (optional feature)......................................56
7.8.2 Pressure difference test (optional feature).............................................................................56
7.8.3 Temperature difference test...................................................................................................58
7.8.4 Determining offset value ........................................................................................................ 59
7.9 Performing freeze-drying using a method [Pro control panel] ........................................................... 59
7.9.1 Preparing the instrument........................................................................................................59
7.9.2 Selecting a method ................................................................................................................ 59
7.9.3 Starting freeze-drying.............................................................................................................60
7.9.4 Ending freeze-drying..............................................................................................................61
7.9.5 Shutting down the instrument ................................................................................................61
7.9.6 Switching off the instrument...................................................................................................61
7.10 Performing freeze-drying manually [Pro control panel]...................................................................... 62
7.10.1 Preparing the instrument........................................................................................................62
7.10.2 Starting freeze-drying.............................................................................................................62
7.10.3 Editing parameters while the process is running ...................................................................63
7.10.4 Ending freeze-drying..............................................................................................................63
7.10.5 Shutting down the instrument ................................................................................................63
7.10.6 Switching off the instrument...................................................................................................64
8 Operating top-mount drying racks ..................................................................................65
8.1 Operating stoppering acrylic drying chamber .................................................................................... 65
8.2 Operating manifold acrylic drying chamber (heatable shelves)......................................................... 68
8.3 Operating manifold acrylic drying chamber (non-heatable shelf) ...................................................... 70
8.4 Operating acrylic drying chamber (heatable shelf) ............................................................................ 72
8.5 Operating acrylic drying chamber (on-heatable shelf)....................................................................... 75
8.6 Operating manifold drying rack.......................................................................................................... 76
8.7 Operating manifold valves ................................................................................................................. 77
9 Cleaning and servicing .....................................................................................................78
9.1 Regular maintenance work................................................................................................................ 78
9.2 Performing a vacuum test.................................................................................................................. 79
9.3 Performing a leak test........................................................................................................................ 80
9.3.1 Performing a leak test with a drying chamber........................................................................80
9.3.2 Performing leak test with a manifold drying rack ...................................................................81
Operation Manual Lyovapor™ L-200/L-200 Pro v
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Table of contents BÜCHI Labortechnik AG
10 Help with faults ..................................................................................................................83
10.1 Troubleshooting................................................................................................................................. 83
10.2 Faults, possible causes and remedies............................................................................................... 83
10.3 Locating leaking manifold valve......................................................................................................... 84
10.4 Finding a leaking regulating valve or aeration valve.......................................................................... 84
11 Taking out of service and disposal.................................................................................. 86
11.1 Disposal............................................................................................................................................. 86
11.2 Returning the instrument ................................................................................................................... 86
12 Appendix ............................................................................................................................87
12.1 Spare parts and accessories ............................................................................................................. 87
12.1.1 Accessories............................................................................................................................87
12.1.2 Further accessories ...............................................................................................................88
12.1.3 Spare parts ............................................................................................................................88
12.1.4 Top-mount drying rack accessories.......................................................................................90
12.1.5 Software.................................................................................................................................93
vi Operation Manual Lyovapor™ L-200/L-200 Pro
Page 7
BÜCHI Labortechnik AG About this document | 1
1 About this document
This operation manual is applicable for all variants of the instrument.
Read this operation manual before operating the instrument and follow the instructions to ensure safe and trouble-free operation.
Keep this operation manual for later use and pass it on to any subsequent user or
owner.
BÜCHI Labortechnik AG accepts no liability for damage, faults and malfunctions resulting from not following this operation manual.
If you have any questions after reading this operation manual:
u Contact BÜCHI Labortechnik AG Customer Service.
https://www.buchi.com/contact
1.1 Connected devices
In addition to these operating instructions, you should also follow the instructions and
specifications in the documentation for the connected devices.
1.2 Warning notices in this document
Warning notices warn you of dangers that can occur when handling the device. There
are four danger levels, each identifiable by the signal word used.
Signal word Meaning
DANGER Indicates a danger with a high level of risk which could result in
WARNING Indicates a danger with a medium level of risk which could result in
CAUTION Indicates a danger with a low level of risk which could result in mi-
NOTICE Indicates a danger that could result in damage to property.
1.3 Symbols
The following symbols may be displayed in this instruction manual or on the device:
1.3.1 Warning symbols
Symbol Meaning
death or serious injury if not prevented.
death or serious injury if not prevented.
nor or medium-severity injury if not prevented.
General warning
Breakable items
Device damage
Operation Manual Lyovapor™ L-200/L-200 Pro 7/94
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1 | About this document BÜCHI Labortechnik AG
1.3.2 Mandatory directive symbols
Symbol Meaning Symbol Meaning
Wear safety goggles Wear protective clothing
Wear protective gloves Heavy load, do not lift with-
out assistance
1.3.3 Mark-ups and symbols
NOTE
This symbol draws attention to useful and important information.
R This character draws attention to a requirement that must be met before the in-
structions below are carried out.
u This character indicates an instruction that must be carried out by the user.
ð This character indicates the result of a correctly carried out instruction.
Mark-up Explanation
Win dow Software Windows are marked-up like this.
Tab Tabs are marked-up like this.
Dia log Dialogs are marked-up like this.
[Button] Buttons are marked-up like this.
[Field names] Field names are marked-up like this.
[Menu / Menu item] Menus or menu items are marked-up like this.
Status Status is marked-up like this.
Signal Signals are marked-up like this.
1.4 Trademarks
Product names and registered or unregistered trademarks that are used in this document are used only for identification and remain the property of the owner in each
case.
8/94 Operation Manual Lyovapor™ L-200/L-200 Pro
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BÜCHI Labortechnik AG Safety | 2
2 Safety
2.1 Proper use
The Lyovapor™ L-200 is used for freeze-drying solid materials in ampoules, vials,
dishes, round-neck or wide-neck flasks and trays is exclusively intended for that purpose. The Lyovapor™ L-200 can be used in laboratories for the following tasks:
Sublimating and re-sublimating water
2.2 Use other than that intended
Use of any other kind than that described in the section Chapter2.1 "Proper use",
page9 and any application that does not comply with the technical specifications
(see Chapter3.6 "Technical data", page17) constitutes use other than that intended.
In particular, the following applications are not permissible:
Use of the instrument in an environment with a potential risk of explosion or areas
which require explosion-safe apparatus.
Use of the instrument for processing substances outside of research and develop-
ment.
Production and processing of substances that can lead to spontaneous reactions,
such as explosives, metal hydrides or solvents that can form peroxides.
Processing with explosive gas mixtures.
Use of acids and alkalis without first checking material compatibility.
Damage or hazards attributable to use of the product other than as intended are entirely at the risk of the operator alone.
2.3 Staff qualification
Unqualified persons are unable to identify risks and are therefore exposed to greater
dangers.
The device may only be operated by suitably qualified laboratory staff.
These operating instructions are aimed at the following target groups:
Users
Users are persons that meet the following criteria:
They have been instructed in the use of the device.
They are familiar with the contents of these operating instructions and the applica-
ble safety regulations and apply them.
They are able on the basis of their training or professional experience to assess
the risks associated with the use of the device.
Operation Manual Lyovapor™ L-200/L-200 Pro 9/94
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2 | Safety BÜCHI Labortechnik AG
Operator
The operator (generally the laboratory manager) is responsible for the following aspects:
The device must be correctly installed, commissioned, operated and serviced.
Only suitably qualified staff may be assigned the task of performing the operations
described in these operating instructions.
The staff must comply with the local applicable requirements and regulations for
safe and hazard-conscious working practices.
Safety-related incidents that occur while using the device should be reported to the
manufacturer (quality@buchi.com).
BUCHI service technicians
Service technicians authorized by BUCHI have attended special training courses and
are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair
measures.
2.4 Location of warning signs on the product
The following warning symbols are present on the instrument.
Fig.1: Location of warning signs on the product
General warning
2.5 Residual risks
The device has been developed and manufactured using the latest technological advances. Nevertheless, risks to persons, property or the environment can arise if the
device is used incorrectly.
Appropriate warnings in this manual serve to alert the user to these residual dangers.
2.5.1 Faults during operation
If a device is damaged, sharp edges or exposed electrical wires can cause injuries.
u Regularly check device for visible damage.
u If faults occur, switch off the device immediately, unplug the power cord and in-
form the operator.
u Do not continue to use devices that are damaged.
10/94 Operation Manual Lyovapor™ L-200/L-200 Pro
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BÜCHI Labortechnik AG Safety | 2
2.5.2 Damage to the ice condenser
Damage to the ice condenser leads to coolant leakage and failure of the instrument.
u Wait until the ice is completely thawed.
u Do not use mechanical means to remove ice from the ice condenser.
2.5.3 Glass and acrylic breakage
Broken glass and acrylic can cause severe cuts.
Damaged glass and acrylic components may implode if subjected to a vacuum.
Minor damage to the ground joints impairs the sealing effect and may therefore diminish sublimination capacity.
u Handle the flask and other glass and acrylic components carefully and do not drop
them.
u Always place the flasks in a suitable holder when they are not mounted on the Ly-
ovapor™.
u Always visually inspect glass and acrylic components for damage every time they
are to be used.
u Do not continue to use glass and acrylic components that are damaged.
u Always wear protective gloves when disposing of broken glass and acrylic.
2.5.4 Low internal pressure
Evacuating the system reduces the pressure in the drying chamber. This reduced
pressure can cause glass and acrylic components to implode.
u Make sure that all glass and acrylic components are free of damage.
2.5.5 Cold and hot surfaces
The condenser coil and probes can be extremely cold. Heatable shelf areas can be
extremely hot. If touched, hot and cold surfaces can cause skin burns.
u Do not touch cold or hot surfaces or liquids and/or wear suitable protective gloves.
2.6 Personal protective equipment
Depending on the application, hazards due to heat and/or corrosive chemicals may
arise.
u Always wear appropriate personal protective equipment such as safety goggles,
protective clothing and gloves.
u Make sure that the personal protective equipment meets the requirements of the
safety data sheets for all chemicals used.
2.7 Modifications
Unauthorized modifications may impair safety and lead to accidents.
u Use only genuine BUCHI accessories, spare parts and consumables.
u Technical modifications to the device or accessories should only be carried out
with the prior written approval of BÜCHI Labortechnik AG and only by authorized
BUCHI technicians.
BUCHI accepts no liability whatsoever for damage arising as a result of unauthorized
modifications.
Operation Manual Lyovapor™ L-200/L-200 Pro 11/94
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3 | Product description BÜCHI Labortechnik AG
3 Product description
3.1 Description of function
The Lyovapor™ is a freeze-dryer in which frozen preparations can be gently dried.
The basis of freeze-drying is sublimation. Sublimation refers to the process whereby a
substance transforms directly from the solid to the gaseous state.
The physical process of sublimation can be explained using the solvent water as an
example.
The water is frozen.
The frozen water is transformed into the gaseous state under vacuum at a pres-
sure below the triple point.
The vacuum prevents the ice melting when heat is applied to the preparation.
Thus, freeze-drying takes place in three phases:
1. Freezing phase: the preparation is frozen at atmospheric pressure.
2. Main drying phase: heat is applied to the frozen preparation under a vacuum.
The frozen water is removed by sublimation.
3. Secondary drying phase (only possible with heatable shelves): the trace levels of water remaining are removed by heating.
The Lyovapor™ consists of an ice condenser and various top-mount drying racks.
The top-mount drying racks can be chosen to suit the availability of the preparation
being dried and the requirements of the end product.
The following top-mount drying racks can be used:
Non-heatable and heatable shelves in rack
Trays in rack
Top-mount rack with manifold valves
3.1.1 Freezing phase
In the freezing phase, the aqueous preparation is transformed to a solid state. Freezing takes place under atmospheric pressure using a separate freezer, a liquid nitrogen bath, or a mixture of dry ice and alcohol.
The end of the freezing phase is reached as soon as the water contained in the
preparation has fully crystallized.
3.1.2 Main drying phase
In the main drying phase, the ice crystals are removed from the preparation by sublimation. Sublimation in the Lyovapor™ takes place under a vacuum with the addition
of thermal energy.
To create the vaccum, the pressure is lowered to the level required for sublimation.
For water that is: less than 6.11mbar.
As the ice condenser is colder than the preparation being dried, the vapour pressure
in the area of the ice condenser is lower than in the area of the preparation. The water vapour escaping from the preparation therefore flows towards the ice condenser.
The water vapour condenses on the coil of the ice condenser.
If a manifold-valve rack is used, heat transfer takes place by convection and radiation
from the surroundings. Control of the thermal energy transferred is then difficult.
12/94 Operation Manual Lyovapor™ L-200/L-200 Pro
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BÜCHI Labortechnik AG Product description | 3
If using a drying chamber with heatable shelves, heat transfer takes place by direct
contact. The temperature of the heatable shelves is controllable. Control of the thermal energy transferred is then possible.
Controlling the heat transfer prevents the following critical temperatures for amorphous and crystalline materials to be reached:
the glass transition temperature Tg' of the frozen preparation
the collapse temperature Tc.
the eutectic temperature Teu.
Above the glass transition temperature and the collapse temperature, the viscosity of
the frozen preparation increases. The increased viscosity leads to the collapse of the
preparation's matrix structure.
Above the eutectic temperature, the preparation melts.
During the main drying phase, the product temperature must remain below the collapse temperature for amorphous materials in the preparation.
Sublimation of the ice crystals progresses downwards from the surface of the product.
Above the sublimation boundary, the product is dry ("freeze-dried cake"), while further
inside the product is still frozen.
The end of the main drying phase is reached when all ice crystals have been removed from the preparation.
After the main drying phase, the remaining fluid content in the preparation can still be
between 5 to 10%.
3.1.3 Secondary-drying phase
In the secondary-drying phase, the unfrozen water is removed from the preparation
by desorption. The secondary-drying function is performed by the heatable shelves in
the drying chamber of the Lyovapor™.
In the secondary-drying phase, the temperature of the heatable shelves is raised and
held for several hours.
The end of the secondary-drying phase is reached when the residual moisture in the
preparation is between 1% and 5%.
Operation Manual Lyovapor™ L-200/L-200 Pro 13/94
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3 | Product description BÜCHI Labortechnik AG
3
5
2
1
4
3
2
1
3.2 Configuration
3.2.1 Front view
Fig.2: Front view Lyovapor™ L-200
1 Connections for heated shelves
(Lyovapor™ L-200 Pro only)
(48 V, max. 2 A)
3 Ventilation slots 4 On/Off master switch
5 User interface
3.2.2 Rear view
2 Ice condenser
Fig.3: Rear viewLyovaporTM L-200
1 Connections on the rear side
2 Drain hose for condensate
(See Chapter3.2.3 "Connections on
the rear side", page15)
3 Ventilation slots
14/94 Operation Manual Lyovapor™ L-200/L-200 Pro
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BÜCHI Labortechnik AG Product description | 3
4
3
14
15
2
1
11
9
8
7
5
6
10
12
13
1
2
3
3.2.3 Connections on the rear side
Fig.4: Connections on rear panel
1 Vacuum pump connection 2 Vacuum connection
3 Type plate 4 Refrigerant details
5 Main valve connection
(24 V, ≤ 0.4 A)
7 Aeration valve connection
(24 V, ≤ 0.4 A)
9 External pressure sensor connection
(24 V, max. 0.125 A)
6 Pressure regulating valve connection
(24 V, ≤ 0.4 A)
8 Spare
(24 V, ≤ 0.4 A)
10 Internal pressure sensor connection
(5 V, max. 0.125 A)
11 LAN port 12 COM port
(24 V, ≤ 0.4 A)
13 Power supply connection 14 Fuse
15 Fuse
3.2.4 Control panel
Fig.5: Control panel
1 View 2 Function buttons
3 Navigation control
Operation Manual Lyovapor™ L-200/L-200 Pro 15/94
Page 16
3 | Product description BÜCHI Labortechnik AG
1
2
Buchi Operations India Private Limited
394230 Surat / India
Type xxx
SN xxxxxxxx
Volt xxx-xxx VAC
Freq xx Hz
Power xxxx W
Built 20xx
Designed by BUCHI in Switzerland
Made in India
6
5
4
1
3
2
7
8
9
10
9
3.2.5 Pro control panel
Fig.6: Pro control panel
1 Touch-screen display 2 Navigation control
3.3 Type plate
The type plate identifies the instrument. The type plate is located on the rear of the instrument.
Fig.7: Type plate
1 Company name and address 2 Instrument name
3 Serial number 4 Input voltage range
5 Frequency 6 Power consumption maximum
7 Year of manufacture 8 Product code
9 Approvals 10 Symbol for "Do not dispose of as
The following instrument names are possible:
L-200
L-200 Pro
household waste"
16/94 Operation Manual Lyovapor™ L-200/L-200 Pro
Page 17
BÜCHI Labortechnik AG Product description | 3
Type: L-xxx xxHz
Cooling System Refrigerant: Rxxx
Filling: xxx g
Global Warming Potential (GWP): xx tCO2
High Side Design Pressure: xxxx
Low Side Design Pressure: xxxx
5
3
1
2
4
6
3.4 Scope of delivery
NOTE
The scope of delivery depends of the configuration of the purchase order.
Accessories are delivered as per the purchase order, order confirmation, and delivery note.
3.5 Refrigerant Specification
The refrigerant details are shown on the back of the instrument.
Fig.8: Refrigerant details
1 Instrument name 2 Refrigerant details
3 Filling capacity 4 Global warming potential
5 High-pressure system design pres-
sure
6 Low-pressure system design pres-
sure
3.6 Technical data
3.6.1 Lyovapor™ L-200
Specification L-200 for 50Hz L-200 for 60Hz
Dimensions without drying attachments (W x D x H)
Weight 75kg 75kg
Minimum clearance on all sides 300mm 300mm
Connection voltage 220-240 VAC 208-230 VAC
Power consumption
(rated)
Power consumption
(maximum)
Lug 10 A/250 V 10 A/250 V
Frequency 50 Hz 60 Hz
Overvoltage category II II
Protection rating IP20 IP20
Pollution degree 2 2
460 × 585 × 510 mm 460 × 585 × 510 mm
1200 W 1200 W
1800 W 1800 W
Condensing capacity
≥ 6 kg / 24 h ≥ 6 kg / 24 h
at 20 °C ambient temperature
Operation Manual Lyovapor™ L-200/L-200 Pro 17/94
Lowest condenser temperature -55 °C -55 °C
Temperature divergence ± 1.0 °C ± 1.0 °C
Page 18
3 | Product description BÜCHI Labortechnik AG
Specification L-200 for 50Hz L-200 for 60Hz
Condenser capacity ≤ 6 kg ≤ 6 kg
Condenser surface area 1410 cm
2
1410 cm
2
Number of compressors 1 1
Refrigerant R507 CFC-free R507 CFC-free
Refrigerant quantity 485 g 467 g
Drying shelf temperature up to 60 °C up to 60 °C
Inert gas pressure 1.1 - 1.2 bar
Drying shelf temperature tolerance ± 1.0 °C ± 1.0 °C
Cooling capacity compressor 1.97 kW 2.33 kW
Vacuum generation time to 0.1
mbar*
Volume-based leakage rate* Typically ≤ 0.001
Minimum system vacuum* Typically ≤ 30 mTorr Typically ≤ 30 mTorr
Optimum control range vacuum* 50 to 500 mTorr 50 to 500 mTorr
* With Pfeiffer Duo 6 vacuum pump / without samples
3.6.2 Ambient conditions
For indoor use only.
Max. altitude above sea level 2000 m
Ambient temperature 15 - 30°C
Maximum relative humidity 80 % for temperatures up to 30°C
Storage temperature max. 45°C
1.1 - 1.2 bar
(max. 2 bar)
(max. 2 bar)
Typically ≤ 10 min Typically ≤ 10 min
Typically ≤ 0.001
mbar x L/sec
mbar x L/sec
3.6.3 Materials
Component Materials
Lyovapor™ housing Steel 1.4301/304 with powder coating
Vacuum chamber and components Steel 1.4301/304
Main connector PE-UHMW 1000
Drying chamber tube and cover PMMA GS
Seals FKM
Manifold drying rack Steel 1.4301/304
Manifold valves Natural rubber, PP
Cooling medium circuit Copper for freezing applications to EN
Vacuum clips Aluminium
Condensate drain tube Silicone
Condensate drain, ventilation and control
valve
Main valve Aluminum with FKM seal
12735-1
Brass with EPDM seal
18/94 Operation Manual Lyovapor™ L-200/L-200 Pro
Page 19
BÜCHI Labortechnik AG Transport and storage | 4
4 Transport and storage
4.1 Transport
NOTICE
Risk of breakage due to incorrect transportation
Make sure that the instrument is fully dismantled.
Pack every instrument components properly to prevent breakage. Use the original
packaging whenever possible.
Avoid sharp movements during transit.
u After transporting, check the instrument and all glass components for damage.
u Damage that has occurred in transit should be reported to the carrier.
u Keep packaging for future transportation.
4.2 Storage
u Make sure that the ambient conditions are complied with (see Chapter3.6 "Tech-
nical data", page17).
u Wherever possible, store the device in its original packaging.
u After storage, check the device, all glass components, seals and tubing for dam-
age and replace if necessary.
4.3 Lifting the instrument
WARNING
Danger due to incorrect transportation
The possible consequences are crushing injuries, cuts and breakages.
u The instrument should be transported by four persons at the same time.
u Lift the instrument at the points indicated.
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1
4
3
Fig.9: Lifting the instrument
1 Instrument 2 Feet
3 Equipment trolley 4 Castor brakes on trolley
Precondition:
R Make sure that the castor brakes on the equipment trolley are locked on.
u Lift the instrument – this requires four persons each lifting at one of the points indi-
cated on the front and rear of the instrument.
u Set the instrument down on the equipment trolley.
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5 Installation
5.1 Installation site
NOTICE
Instrument damaged if switched on too early.
After transporting, wait twelve hours before switching on the instrument. The fluid in
the cooling system requires twelve hours to collect in the refrigerant compressor.
The installation site must meet the following requirements:
Firm, level surface
Minimum space requirement: 520 mm x 645 mm x 510 mm (W x D x H).
Take into account the maximum product dimensions and weight.
Take into account the 1100 mm operating height of the drying attachments.
Clearance on each side of the instrument must be at least 30 cm. This clearance
ensures air circulation and prevents the instrument from overheating.
Do not place loose papers or cloths below or to the sides of the instrument, as
these could impede the air circulation if drawn in.
Operate the instrument at an ambient temperature of +15°C to +30°C.
Do not expose the instrument to any external thermal loads, such as direct solar
radiation.
When installing the instrument, make sure that the feet do not bend.
When mounting on an instrument cart, place the feet of the instrument into the sup-
ports on the instrument cart.
NOTE
Make sure that the power supply can be interrupted at any time during an emergency.
5.2 Securing against earthquakes
The Lyovapor™ L-200 has an earthquake fixing point to protect the device against
falling.
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Fig.10: Lyovapor™ L-200
1 Lashing mount
u Tie the lashing mount to a fixed point using strong cord or a wire.
5.3 Putting the instrument in operation
NOTICE
Instrument damaged if restarted too early
Wait ten minutes before restarting the instrument. The oil in the refrigerant compressor requires ten minutes to return to the collection tank.
5.3.1 Preparing the instrument
u Clean the instrument with a damp cloth before commissioning.
u Check all sealing surfaces for scratches, dust, and cleanliness.
5.3.2 Establishing electrical connections
NOTE
Observe the legal requirements when connecting the instrument to the power supply.
4 Use additional electrical safety features (e.g., residual-current circuit breakers) to
comply with local laws and regulations.
The power supply must fulfil the following conditions:
1. Provide the mains voltage and frequency specified on the type plate of the instrument.
2. Be designed for the load imposed by the instruments connected.
3. Be equipped with suitable fuses and electrical safety features.
4. Be equipped with proper earthing.
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NOTICE
Risk of property damage and diminished performance due to use of unsuitable power cables.
The power supply cables supplied with the product by BUCHI precisely match the requirements of the device. If other power cables that do not meet those requirements
are used, the device may be damaged and/or its performance diminished.
u Use only the power supply cables supplied with the product or ordered separately
from BUCHI.
u If using any other power supply cables, make sure that they match the specifica-
tions on the type plate.
u Make sure that all connected devices are earthed.
u If an extension lead is required, make sure that it is earthed and has a suitable
power rating.
u Make sure that the power plug is freely accessible at all times.
u Plug the power cable into the connection labelled Power IN on the back of the in-
strument.
u Plug the mains plug into the power socket.
5.3.3 Fitting the advanced vacuum control unit
u Fit the 90° elbows onto the cross-pipe.
u Fit the aeration valve onto the 90° elbow.
Precondition:
R The arrow is pointing in the direction of the
vacuum pump.
u Fit the main valve onto the cross-pipe.
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u Fit the pressure-regulating valve onto the 90°
elbow.
u Fit the assembled advance vacuum control
unit onto the instrument.
u Plug the aeration valve connector into the
socket marked Aeration Valve.
u Plug the pressure-regulating valve connector
into the socket marked Regulation valve.
u Plug the main valve connector into the socket
marked Main Valve.
5.3.4 Manual switch on the main valve
Use the main valve manual switch to set the vacuum control mode.
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Fig.11: Manual switch on main valve
1 Main valve 2 Manual switch on main valve
Manual switch positions
Manual switch position
Meaning
Precondition:
R Pressure-regulating valve and venting valve are connected.
u The vacuum is regulated by the pressure-regulating valve
and venting valve.
Precondition:
R Pressure-regulating valve and venting valve are not con-
nected.
u The vacuum is regulated by the main valve.
Precondition:
R The main valve manual switch is depressed.
u As long as the main valve manual switch is depressed,
vacuum regulation is deactivated.
The vacuum is not regulated.
5.3.5 Connecting the inert gas (optional feature)
Precondition:
R Make sure that the pressure of the inert gas
matches the technical specifications. See
Chapter3.6 "Technical data", page17
u Fit the inert gas tube onto the gas connection
on the pressure regulating valve.
u Fix the inert gas tube with the tube clip.
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5.3.6 Assembling pressure sensor PPG010 (optional accessory)
The pressure sensor measures the pressure in the ice condenser.
To protect against damage in transit, the pressure sensor is supplied pre-calibrated in
the original packaging.
Fig.12: Fitting the pressure sensor
1 Pressure sensor PPG010 2 Clamp, ISO-KF 16
3 Seal, ISO-KF 16 4 Connection, ISO-KF 16
u Switch the On/Off master switch to Off.
u Remove the shipping cap from the connection (4).
u Fit the pressure sensor (1) and seal (3) to the connection (4) and fix with the
clamp (2).
u Plug the pressure sensor electrical connection into the socket marked Internal
Vacuum Sensor.
u Select the sensor from the submenu [Settings] on the control panel.
5.3.7 Assembling the alternative pressure sensor (optional accessory)
Instead of the standard pressure sensor, an alternative pressure sensor can be used.
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Fig.13: Fitting alternative pressure sensor
1 Connection, ISO-KF 16 2 External pressure sensor connection
u Switch the On/Off master switch to Off.
u Remove the blanking cap from the connection (1).
u Connect the pressure sensor to the connection (1).
u Plug the pressure sensor connecting lead into the socket marked External Vac-
uum Sensor (2).
u Select the sensor from the submenu [Settings] on the control panel.
5.3.8 Fitting the drain valve strainer
u Place the drain valve strainer in the
waste outlet in the base of the ice
condenser.
5.3.9 Preparing the condensate drain hose
CAUTION
Risk of scalding by hot water
u Make sure the condensate drain hose is not loose.
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1
2
3
NOTICE
Contamination of the device
Escaping condensate can contaminate the instrument.
u Fit the condensate drain hose with a downward slope. Make sure that the drain
hose is not immersed in the condensate.
u Seal the condensate drain hose with the end plug as soon as cleaning of the in-
strument is completed.
Fig.14: Condensate drain hose
1 Rubber ring 2 Drain hose for condensate
3 End plug
The condensate drain hose is on the side of the instrument. The drain hose is sealed
with an end plug. The condensation is drained off through this drain hose after completion of the freeze-drying process.
u Pull the drain hose (2) together with end plug (3) out of the side panel of the instru-
ment.
u Remove the end plug.
u Direct the drain hose into a waste outlet or place a container underneath it.
5.4 Commissioning the vacuum pump
The vacuum pump evacuates the top-mount drying rack during the freeze-drying
process.
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NOTICE
Closed gas ballast valve when using solvent.
A closed gas ballast valve when using solvents can cause damage to the instrument.
u When using solvents, keep the gas ballast valve open.
NOTE
To increase the service life of the vacuum pump, operate the vacuum pump with an
open gas ballast valve.
NOTE
Prepare the vacuum pump according to the manufacturer's instructions. See relevant
documentation.
Fig.15: Connections for vacuum pump
1 Vacuum pump connection 2 Vacuum tube connection,
ISO-KF 25
u Switch the On/Off master switch to Off.
u Connect the vacuum pump vacuum tube to the vacuum tube connection (2).
u Plug the vacuum pump electrical connection into the socket marked Vacuum
Pump.
5.5 Establishing LAN connection
5.5.1 Requirements for local network settings
u The following port has to be enabled in the firewall settings on the internet gate-
way:
TCP (HTTPS) traffics through remote port 443
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u In order to use the BUCHI Cloud a DNS server must be configured on the instru-
ment.
NOTE
If there is no DNS server available enter the IP address for the BUCHI Cloud connection manually.
NOTE
If there is no DHCP server available enter the IP address, gateway subnet mask and
DNS server manually.
5.5.2 Preparing the instrument for app using
NOTICE!Do not unplug the LAN cable
while the device is connected to the
BUCHI Cloud Services
u Connect the instrument with the LAN
network.
Navigation path
➔ ➔ [Settings] ➔ [Network]
u Navigate to the action [Network].
u Enable the function [DHCP].
ð The instrument is prepared.
5.5.3 Enabling BUCHI Cloud access
Enable access to BUCHI Cloud in order to use the BUCHI Monitor App.
Navigation path
➔ ➔ Settings ➔ Network ➔ BUCHI Cloud
u Navigate to the action [[BUCHI Cloud]] via the navigation path.
u Select the option [Yes].
ð The instrument is connected to the BUCHI Cloud.
5.6 Insert SD card (Pro control panel only)
NOTE
Only insert or remove the SD card in Standby mode.
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u Fold the control panel forward.
u Insert the SD card on the underside.
u Switch on the instrument.
ð The status bar shows the SD
card symbol.
The following data is stored on the SD card:
Numbering
Date
Time
Set pressure
Current pressure in the ice condenser
Inlet temperature of the ice condensers
Set drying shelf temperature
Current temperature of the drying shelves
Current sample temperatures
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6
5
6 Operating the control panel
This section describes the operation of the instrument using the control panel.
CAUTION
Risk of injury from glass splinters
Sharp objects can damage the display.
u Keep sharp objects away from the display.
6.1 Layout of the control panel
Fig.16: Layout of the control panel
No. Description Function
1 Status bar Shows the current status of the instrument.
2 Menu bar Shows symbols representing the menus.
3 Content area Shows current settings, submenus or actions
4 Function bar Shows functions that can be performed ac-
5 Navigation control Used for navigating the user interface.
6 Function buttons Pressing a function button performs the as-
6.2 Function bar
The function bar shows available functions according to the current operation.
The functions on the function bar are executed by tapping the relevant function buttons or pressing the navigation control.
depending on current operation.
cording to the current operation.
Pressing the control performs the assigned
function on the function bar.
signed function on the function bar.
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E S C
O K
E D I T
M E N U
S AV E
OFF
S TAR T
SKIP
General function buttons
Symbol Description Meaning
[Back] The display reverts to the previous
view.
[Cancel] Cancels an operation.
[Add to favourites] Adds the selected item to the
[[Favourites]] menu.
[Remove from
favourites]
[Confirm] Confirms an entry.
[Edit] Allows the selected item to be
[Menu] Allows selection of a menu from the
[Save] Saves the setting.
Process control function buttons
Symbol Description Meaning
[Defrost] Defrosts the ice condenser.
[Aerate] Vents the system.
[Shut down] The instrument shuts down.
[[Start]] Starts the freeze-drying process.
Removes the selected item from the
[Favourites] menu.
edited.
menu bar using the navigation control.
[Start conditioning] Starts the conditioning phase.
[[Skip]] Skips the current process.
6.3 Menu bar
The menus are represented by symbols on the menu bar. Navigation through the
menus is by input controls.
The following menus are available:
Menu symbol
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Meaning Submenu/Action
[Start] menu Process control parameters
[Favourites] menu Bookmarks for individual entry points
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6 | Operating the control panel BÜCHI Labortechnik AG
Menu symbol
6.3.1 Start menu
On the [Start] menu, parameters can be set manually.
Changing parameters
u Select a parameter by turning the navigation control.
ð The control panel highlights the selected parameter in green.
u Tap the function [Edit] on the function bar.
ð The control panel highlights the selected parameter in black.
u To increase or decrease the figure, turn the navigation control clockwise or anti-
clockwise.
u Tap the function [Save] on the function bar.
ð The setting is saved.
Meaning Submenu/Action
[Configuration] menu Process settings
Settings
Servicing
Service
System information
[Messages] menu Notifications
Logbook
6.3.2 Favourites menu
The [Favourites] menu allows you to define submenus and actions as bookmarks.
Adding a favourite
u Navigate to a submenu or action.
u Tap the function [Add to favourites] on the function bar.
ð The user interface switches to the [Favourites] menu and displays the
favourite created.
Removing a favourite
u On the [Favourites] menu, navigate to the favourite you wish to remove.
u Tap the function [Remove from favourites] on the function bar.
ð The favourite is removed.
6.3.3 Configuration menu
On the [Configuration] menu, you can enter a variety of settings and retrieve information.
Process settings submenu
The submenu [Process Settings] contains functions for automatic process control.
Action Option Explanation
[Vacuum test after
conditioning]
Off/On Automatic vacuum test after the con-
ditioning phase
Settings submenu
The submenu [Settings] contains system settings for the instrument.
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Action Option Explanation
[Mobile connection
password]
View The control panel shows a password
for entry in the BUCHI Monitor app.
[Mobile connection
QR code]
View The control unit shows a QR code
for reading by the BUCHI Monitor
app.
[Language] Choice of display lan-
guage on the control
panel
The following languages are available:
English/German/French/Italian/
Spanish/Russian/Portuguese/Japanese/Chinese/Indonesian/Korean
[Temperature unit] Choice of unit for indi-
cation of temperatures
The following units are available:
°C (Celsius)/°F (Fahrenheit)/K
(Kelvin)
[Pressure unit] Choice of unit for indi-
cation of the vacuum
The following units are available:
HPa (hectopascals), mbar (mil-
libars), torr (= torr), mTorr (= millitorr), mmHg (millimetres of mercury)
[Date] Date input Enter in sequence: Day, month,
year. Apply the settings by pressing
[Save].
[Time] Time input Enter in sequence: Minutes, hours.
Apply the settings by pressing
[Save].
[Pressure sensor] Pressure sensor selec-
tion
The following pressure sensors are
available:
None, Inficon PPG010, Inficon
Porter CDG020D, Inficon PSG 550
[Vacuum pump oil
change]
Enter value Enter the oil change interval recom-
mended by the manufacturer.
[Button tone] Off/On Setting for audible signal in re-
sponse to input controls.
[Display brightness] Enter setting Display illumination level in %: 0 -
100
[Network] Enter value The following parameters can be
edited:
Device name/MAC address/DHCP/
System IP address/Subnet mask/
Gateway/DNS server/BUCHI Cloud/
Server IP address
[Delete app connection]
Confirmation question Resets external connections to the
instrument.
Submenu Maintenance
The submenu [Maintenance] contains tests for maintaining the instrument.
Action Option Explanation
[Vacuum test] Perform vacuum test See Chapter9.2 "Performing a vac-
uum test", page79
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Action Option Explanation
[Leak test] Perform leak test See Chapter9.3.1 "Performing a
leak test with a drying chamber",
page80
Submenu Service
NOTE
While freeze-drying is in progress, no settings can be changed on the Service submenu.
Action Option Explanation
[Refrigerant circuit] View The following information on the re-
frigerant circuit is available:
Hours of duty
Compressor
Ice condenser inlet temperature
Ice condenser outlet temperature
High-pressure safety cut-out
Low-pressure safety cut-out
[Vacuum system] View The following information on the vac-
uum pump is available:
Pump hours of duty
Pump oil hours of duty
Pressure in the ice condenser
Main valve
Vacuum pump
Venting valve
Regulating valve
[Defrosting system] Display/entry of figures The following information on the de-
frosting system is available:
Hours of duty
Drain valve open/closed
Submenu System Information
The submenu [System information] contains details of the connected components
and information on network connection diagnosis.
Action Option Explanation
[Control panel] View The following information on the
control panel is available:
Serial number
Firmware version
Hours of duty
Status
PCB Temperature
24V power supply
5V power supply
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Action Option Explanation
[L-200] View The following information on the
L-200 is available:
Serial number
Firmware version
Hours of duty
Status
PCB Temperature
48V power supply
24V power supply
5V power supply
3.3V power supply
[Network diagnostics]
6.3.4 Messages menu
The [Messages] menu shows the current instrument messages and the instrument's
message history.
The following message types are possible:
I = Information
W = Warning
E = Error
Submenu Notifications
The submenu [Notifications] shows a list of unacknowledged and unresolved notifications together with date and time in each case.
Submenu Logbook
The submenu [Logbook] shows the instrument's message history.
Logbook:
List of the last 100 messages.
Every occurrence is shown with date and time.
View/Enter settings The following network diagnosis in-
formation is available:
Network interruptions
Event list
The following status types are possible:
Symbol Description Meaning
x Acknowledged The message has been be processed
and acknowledged.
< Sent The message initiator is no longer
present.
> Received The display shows a message.
6.4 Status bar
The status bar shows the status of the instrument.
The following statuses are possible:
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Indication on status bar
View Status
Unload / Load Before the freeze-drying process:
Load the top-mount drying rack with a frozen
sample.
After the freeze-drying process:
Remove the dried sample from the top-mount
drying rack.
Aerating The system is venting.
Shutting down The instrument is shutting down.
The aeration valve and the drain valve are
closed.
The status bar shows the remaining time.
Defrosting The instrument is defrosting.
The aeration valve and the drain valve are
open.
The status bar shows the remaining time.
Manual defrosting with water possible.
Standby The instrument is in energy-saving mode.
Conditioning The instrument is starting up.
Warming up pump The vacuum pump is being brought up to operat-
ing temperature.
Vacuum Test The instrument is performing a vacuum test.
Leak Test The instrument is performing a leak test.
Manual Drying The instrument is in the course of a manual
freeze-drying process.
Recovering The system is in the process of recovering from a
power failure.
The current parameters of the freeze-drying
process are being re-established.
Reconditioning The instrument is restarting after a temporary
power failure.
Symbols on the status bar
Symbol Status
The instrument is connected to the BUCHI Cloud.
The instrument is defrosting.
The instrument is starting up.
The instrument is in energy-saving mode.
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Symbol Status
Before the freeze-drying process:
Load the top-mount drying rack with a frozen
sample.
After the freeze-drying process:
Remove the dried sample from the top-mount
drying rack.
The system evacuates to the set pressure.
The instrument is performing a vacuum test or a
leak test.
6.5 Carrying out freeze-drying
6.5.1 Preparing the instrument
Time
required:
approx.
30min
NOTE
To minimize the condensation of moisture from the air in the condenser, place a drying rack.
Navigation path
➔ Start
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Start conditioning] on the function bar.
ð The temperature in the ice condenser decreases to operating temperature.
ð The vacuum pump is brought up to operating temperature.
ð After completion of the conditioning phase, the status bar shows the status
Unload / Load.
6.5.2 Starting freeze-drying
CAUTION
Risk of skin burns from touching parts of the ice condenser after completion of conditioning.
u Wear protective gloves when working on the instrument after the conditioning
phase.
Navigation path
➔ Start
Precondition:
R The instrument has been prepared.
u Fit a top-mount drying rack. See Chapter8 "Operating top-mount drying racks",
page65.
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u Load the top-mount drying rack with frozen samples.
u Navigate to the [Start] menu via the navigation path.
u Enter the required settings for the process parameters.
u Tap the function [Start] on the function bar.
ð The freeze-drying process starts.
ð The control panel shows the S tar t menu with a black background.
ð The status bar shows a clock counting up and the status Manual Drying.
ð The system evacuates to the set pressure.
6.5.3 Editing parameters while the process is running
Navigation path
➔ Start
Precondition:
R The freeze-drying process has been started.
u Navigate to the [Start] menu via the navigation path.
u Navigate to the desired parameter using the navigation control.
u Tap the function [Edit] on the function bar.
ð The control panel highlights the selected parameter in white.
u Turn the navigation control to increase or decrease the parameter setting.
u Tap the function [Save] on the function bar.
ð The setting is saved.
6.5.4 Ending freeze-drying
Navigation path
➔ Start
Precondition:
R The sample is dry.
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Aerate] on the function bar.
u Answer YES to the confirmation question.
ð The system is vented.
ð The status bar shows the status Aerating.
u As soon as the status bar shows the status Unload / Load, remove the dried sam-
ple from the drying rack.
6.5.5 Shutting down the instrument
Time
40min
required:
NOTICE
Do not use force to remove the ice from the ice condenser.
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Navigation path
➔ Start
Precondition:
R The freeze-drying process has ended.
u Position the condensate drain hose so that the condensate can be drained off.
See Chapter5.3.9 "Preparing the condensate drain hose", page27
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Shut down] on the function bar.
ð The instrument is shutting down.
ð The status bar shows the remaining time and the status Defrosting.
ð After the instrument has shut down, the status bar shows the remaining time
and the status Shutting down.
6.5.6 Switching off the instrument
Precondition:
R The instrument has been shut down. See Chapter6.5.5 "Shutting down the instru-
ment", page40
u Switch the On/Off master switch to Off.
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4
3
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7 Operating Pro control panel
This section describes the operation of the instrument using the Pro control panel.
CAUTION
Risk of injury from glass splinters
Sharp objects can damage the display.
u Keep sharp objects away from the display.
7.1 Layout of Pro control panel
Fig.17: Layout of Pro control panel
No. Description Function
1 Status bar Shows the current status of the instrument.
2 Menu bar Shows symbols representing the menus.
3 Content area Shows current settings, submenus or actions
4 Function bar Shows functions that can be performed ac-
5 Navigation control Used for navigating the user interface. Press-
7.2 Function bar
The function bar shows functions that can be performed according to the current operation.
The functions on the function bar are executed by tapping the relevant function buttons or pressing the navigation control.
General function buttons
depending on the current operation.
cording to the current operation
ing the control performs the assigned function on the function bar.
Symbol Description Meaning
[Back] The display reverts to the previous
view.
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E S C
O K
E D I T
M E N U
S AV E
OFF
S TAR T
SKIP
DELETE
Symbol Description Meaning
[Cancel] Cancels an operation.
[Add to favourites] Adds the selected item to the
[[Favourites]] menu.
[Remove from
favourites]
[Confirm] Confirms an entry.
[Edit] Allows the selected item to be
[Menu] Allows selection of a menu from the
[Save] Saves the setting.
Process control function buttons
Symbol Description Meaning
[Defrost] Defrosts the ice condenser.
[Aerate] Vents the system.
[Shut down] The instrument shuts down.
[[Start]] Starts the freeze-drying process.
Removes the selected item from the
[Favourites] menu.
edited.
menu bar using the navigation control.
[Start conditioning] Starts the conditioning phase.
[Manual] Switches to manual freeze-drying.
[Method] Switches to freeze-drying with pro-
grammable parameters.
[New] Creates a new method
[Right] Moves the selection to the right.
[Left] Moves the selection to the left.
[Progression] Graphical display of method pro-
gression showing pressure and temperature details.
[Activate] Confirms selection of a method.
[[Skip]] Skips the current process.
[Delete] Deletes the selected method or step.
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C O P Y
Symbol Description Meaning
[Copy] Copies the selected method.
7.3 Other symbols on the control panel
Symbol Description Meaning
[Closed] The assigned method is active and
cannot be changed.
7.4 Menu bar
The menus are represented by symbols on the menu bar. Navigation through the
menus is by the input controls.
The following menus are available:
Menu symbol
7.4.1 Start menu
On the [Start] menu, parameters can be set manually.
Meaning Submenu/Action
Start menu Process control parameters
Favourites menu Bookmarks for individual entry points
Method menu For saving freeze-drying methods
Editing and activating freeze-drying
method
Configuration menu Process settings
Settings
End point determination
Maintenance
Service
System information
Messages menu Notifications
Logbook
Setting parameters using the navigation control
u Select a parameter by turning the navigation control.
ð The control panel highlights the selected parameter in green.
u Tap the function [Edit] on the function bar.
ð The control panel highlights the selected parameter in black.
u To increase or decrease the figure, turn the navigation control clockwise or anti-
clockwise.
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u Press the navigation control.
ð The setting is saved.
ð The control panel highlights the new setting in green.
Setting parameters using the touch-screen
u Select the parameter by tapping the control panel screen.
ð The control panel shows a dialog box with a numeric input box.
ð The control panel highlights the selected parameter in black.
u Enter the value in the numeric input box.
u Tap the function [Save] on the function bar.
ð The setting is saved.
ð The dialog box closes.
ð The control panel highlights the new setting in green.
7.4.2 Favourites menu
The [Favourites] menu allows you to define submenus and actions as favourites.
Adding a favourite
u Navigate to a submenu or action.
u Tap the function [Add to favourites] on the function bar.
ð The user interface switches to the [Favourites] menu and displays the
favourite created.
Removing a favourite
u On the [Favourites] menu, navigate to the favourite you wish to remove.
u Tap the function [Remove from favourites] on the function bar.
ð The favourite is removed.
7.4.3 Method menu
The [Method] menu allows freeze-drying processes with multiple phases and steps to
be saved. See Chapter7.6 "Editing a method", page50
7.4.4 Configuration menu
On the [Configuration] menu, you can enter a variety of settings and retrieve information.
Process settings submenu
The submenu [Process Settings] contains actions for automatic process control.
Action Option Explanation
[Vacuum test after
conditioning]
[Leak test after
conditioning]
Off/On Automatic vacuum test after the con-
ditioning phase
Off/On Automatic leak test after the condi-
tioning phase
Settings submenu
The submenu [Settings] contains system settings for the instrument.
Action Option Explanation
[Mobile connection
password]
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View The control panel shows a password
for entry in the BUCHI Monitor app.
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Action Option Explanation
[Mobile connection
QR code]
View The control unit shows a QR code
for reading by the BUCHI Monitor
app.
[Language] Choice of display lan-
guage on the control
panel
The following languages are available:
English/German/French/Italian/
Spanish/Russian/Portuguese/Japanese/Chinese/Indonesian/Korean
[Temperature unit] Choice of unit for indi-
cation of temperatures
The following units are available:
°C (Celsius)/°F (Fahrenheit)/K
(Kelvin)
[Pressure unit] Choice of unit for indi-
cation of the vacuum
The following units are available:
HPa (hectopascals), mbar (mil-
libars), torr (= torr), mTorr (= millitorr), mmHg (millimetres of mercury)
[Date] Date input Enter in sequence: Day, month,
year. Apply the settings by pressing
[Save].
[Time] Time input Enter in sequence: Minutes, hours.
Apply the settings by pressing
[Save].
[Pressure sensor] Pressure sensor selec-
tion
The following pressure sensors are
available:
None, Inficon PPG010, Inficon
Porter CDG020D, Inficon PSG 550
[Vacuum pump oil
change]
Enter value Enter the oil change interval recom-
mended by the manufacturer.
[Button tone] Off/On Setting for audible signal in re-
sponse to input controls.
[Display brightness] Enter setting Display illumination level in %: 0 -
100
[Network] Enter value The following parameters can be
edited:
Device name/MAC address/DHCP/
System IP address/Subnet mask/
Gateway/DNS server/BUCHI Cloud/
Server IP address
[Delete app connection]
Confirmation question Resets external connections to the
instrument.
Submenu End point determination
Action Option Explanation
[Pressure difference test]
[Temperature difference test]
View Actual and specified settings
Result
View Actual and specified settings
Result
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Submenu Maintenance
The submenu [Maintenance] contains tests for maintaining the instrument.
Action Option Explanation
[Vacuum test] Perform vacuum test See Chapter9.2 "Performing a vac-
uum test", page79
[Leak test] Perform leak test See Chapter9.3.1 "Performing a
leak test with a drying chamber",
page80
Submenu Service
NOTE
While freeze-drying is in progress, no settings can be changed on the Service submenu.
Action Option Explanation
[Refrigerant circuit] View The following information on the re-
frigerant circuit is available:
Hours of duty
Compressor
Ice condenser inlet temperature
Ice condenser outlet temperature
High-pressure safety cut-out
Low-pressure safety cut-out
[Vacuum system] View The following information on the vac-
uum pump is available:
Pump hours of duty
Pump oil hours of duty
Pressure in the ice condenser
Main valve
Vacuum pump
Venting valve
Regulating valve
[Defrosting system] Display/entry of figures The following information on the de-
frosting system is available:
Hours of duty
Drain valve open/closed
[Drying shelves] View Switches heating for the individual
shelves on and off (where available).
Submenu System Information
The submenu [System information] contains details of the connected devices and information on network connection diagnosis.
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Action Option Explanation
[Control panel] View The following information on the
control panel is available:
Serial number
Firmware version
Hours of duty
Status
PCB Temperature
24V power supply
5V power supply
[L-200] View The following information on the
L-200 is available:
Serial number
Firmware version
Hours of duty
Status
PCB Temperature
48V power supply
24V power supply
5V power supply
3.3V power supply
[Network diagnostics]
7.4.5 Messages menu
The [Messages] menu shows the current instrument messages and the instrument's
message history.
The following message types are possible:
I = Information
W = Warning
E = Error
Submenu Notifications
The submenu [Notifications] shows a list of unacknowledged and unresolved notifications together with date and time in each case.
Submenu Logbook
The submenu [Logbook] shows the instrument's message history.
Logbook:
List of the last 100 messages.
Every occurrence is shown with date and time.
View/Enter settings The following network diagnosis in-
formation is available:
Network interruptions
Event list
The following status types are possible:
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Symbol Description Meaning
x Acknowledged The message has been be processed
and acknowledged.
< Sent The message initiator is no longer
present.
> Received The display shows a message.
7.5 Status bar
The status bar shows the status of the instrument.
The following statuses are possible:
Indication on status bar
Unload / Load Before the freeze-drying process:
Load the top-mount drying rack with a
frozen sample.
After the freeze-drying process:
Remove the dried sample from the top-
mount drying rack.
Aerating The system is venting.
Shutting down The instrument is shutting down.
The aeration valve and the drain valve
are closed.
The status bar shows the remaining
time.
Defrosting The instrument is defrosting.
The aeration valve and the drain valve
are open.
The status bar shows the remaining
time.
Manual defrosting with water possible.
Standby The instrument is in energy-saving
mode.
Conditioning The instrument is starting up.
Reconditioning The instrument is restarting after a tem-
porary power failure.
Warming up pump The vacuum pump is being brought up to
operating temperature.
Vacuum Test The instrument is performing a vacuum
test.
Leak Test The instrument is performing a leak test.
Manual Drying The instrument is in the course of a man-
ual freeze-drying process.
Recovering The system is in the process of recover-
ing from a power failure.
The current parameters of the freeze-
drying process are being re-established.
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Hold The instrument is in the holding phase.
Primary drying The instrument is in the primary drying
phase.
Secondary drying The instrument is in the secondary dry-
ing phase.
Tempering shelves The instrument is modulating the heat-
able shelves to the set temperature.
Stoppering The instrument is ready for stoppering.
Symbols on the status bar
Symbol Status
The instrument is defrosting.
The instrument is in energy-saving mode.
The instrument is in the course of a freeze-drying
process using a method.
The instrument is starting up.
7.6 Editing a method
The Pro control panel can save up to 35 methods. The methods enable the freezedrying process to be automated.
The instrument is in the course of a manual
freeze-drying process.
The instrument is connected to the BUCHI Cloud.
Before the freeze-drying process:
Load the top-mount drying rack with a frozen
sample.
After the freeze-drying process:
Remove the dried sample from the top-mount
drying rack.
The instrument is performing a vacuum test or a
leak test.
The system evacuates to the set pressure.
The memory card has been inserted.
7.6.1 Creating a new method
There are two possible ways of creating a new method:
Creating a new method
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
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u Tap the function [New] on the function bar.
ð The new method is created.
Creating a new method by copying an existing method
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to copy.
u Tap the function [Copy] on the function bar.
ð The new method is created.
7.6.2 Changing the name of a method
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the action [Information].
ð The control panel shows the action Information.
u Tap the setting [Name].
ð The control panel shows a blank box with an alphanumeric input box.
u Enter a name for the method.
u Tap the function [Save] on the function bar.
ð The new name is saved.
ð The dialog box closes.
7.6.3 Setting the sample collapse temperature
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the action [General].
ð The control panel shows the action [General].
u Tap the setting [Sample collapse temperature].
ð The control panel shows a dialog box with a numeric input box.
u Enter the value in the numeric input box.
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u Tap the function [Save] on the function bar.
ð The setting is saved.
ð The dialog box closes.
7.6.4 Setting the gas type
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the action [General].
ð The control panel shows the action [General].
u Tap the setting [Gas type].
ð The control panel shows a dialog box with an alphanumeric input box.
u Enter the gas type.
u Tap the function [Save] on the function bar.
ð The setting is saved.
ð The dialog box closes.
7.6.5 Setting the shelf loading temperature
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the action [General].
ð The control panel shows the action [General].
u Tap the action [Shelf load temp.]
ð The control panel shows a dialog box with a numeric input box.
u Enter the value in the numeric input box.
u Tap the function [Save] on the function bar.
ð The setting is saved.
ð The dialog box closes.
7.6.6 Setting the steps of a method
The Pro control panel can save up to 30 steps for each method.
NOTE
The maximum heating rate is 3 °C/min.
NOTE
The settings for the action Steps affect a single step in each case.
Navigation path
➔ Method
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u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the action [Steps].
ð The control panel shows the action Steps.
The following settings are available for each step:
Setting Option Meaning
[Step phase] Primary drying/Sec-
Sets the type of step phase.
ondary drying
[Term] Enter value Sets the duration of the step in
minutes.
[Shelf temperature] Enter value Set the temperature of the heat-
able shelves in one step.
[Pressure zone] Regulated/Minimum Regulated: the settings for pres-
sure and pressure limits are applied.
Minimum: The maximum vacuum is applied to reach the lowest possible pressure.
[Pressure] Enter value Sets a target value for the regu-
lated pressure.
[Pressure limit] Enter value Absolute value for divergence
from the set pressure before the
sample protection function is activated.
[Pressure duration] Enter value Sets the period of time that the
pressure is allowed to exceed
the pressure limit before the
sample protection function is activated.
Editing a step
u Use the function [Right] or [Left] on the function bar to navigate to the step that
you wish to edit.
u Using the navigation control, navigate to the setting that you wish to change.
u Tap the function [Edit] on the function bar.
u Edit the setting as required.
u Tap the function [Save] on the function bar.
ð The setting is changed.
Add step
u Use the function [Right] or [Left] on the function bar to navigate to the position at
which you wish to add a step.
u Tap the function [New] on the function bar.
ð The new step is created.
Delete step
u Use the function [Right] or [Left] on the function bar to navigate to the step that
you wish to delete.
u Tap the function [Delete] on the function bar.
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u When asked to confirm, press [OK].
ð The step is deleted.
7.6.7 Setting the phases of a method
NOTE
The settings in the Phase view affect all steps of a phase.
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the action [Phase].
ð The control panel shows the Phase view.
The following phases of a method are available:
Phase Setting Option Meaning
[Primary
drying]
[Pressure action]
None/Sample
protection/
Message
None: no action is carried out.
Sample protection: if the pressure is
too high, heating of the shelves is
paused.
[Temp. action] None/Sample
protection/
Message
[Safety temper-
Enter value Maximum divergence from the set
ature]
[Safety temp.
Enter value Time as of which sample protection
Time]
[End point defi-
More settings See Chapter7.8 "Setting end point
nition]
Message: if the pressure is too high,
the control panel displays a message.
None: no action is carried out.
Sample protection: if the temperature is too high, heating of the
shelves is paused.
Message: if the temperature is too
high, the control panel displays a
message.
collapse temperature before the
sample protection function is activated.
is inactive. The value relates to the
time before completion of primary
drying.
definitions", page56
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Phase Setting Option Meaning
[Secondary
drying]
[Pressure action]
None/Sample
protection/
Message
None: no action is carried out.
Sample protection: if the pressure is
too high, heating of the shelves is
paused.
Message: if the pressure is too high,
the control panel displays a message.
[Stoppering]
[Temp. action] None/Sample
protection/
Message
None: no action is carried out.
Sample protection: if the temperature is too high, heating of the
shelves is paused.
Message: if the temperature is too
high, the control panel displays a
message.
[Safety temperature]
Enter value Maximum divergence from the set
shelf temperature before the sample
protection function is activated.
[End point definition]
[Pressure
zone]
More settings See Chapter7.8 "Setting end point
definitions", page56
Regulated/Minimum
Regulated: the settings for pressure
and pressure limits are applied.
Minimum: The maximum vacuum is
applied to reach the lowest possible
pressure.
[Pressure] Enter value Sets a target value for the regulated
pressure.
[&Mode] None/Manual None: no action is carried out.
Manual: sealing is performed manually.
[hold] [Pressure
zone]
Regulated/Minimum
Regulated: the settings for pressure
are applied.
Minimum: The maximum vacuum is
applied to reach the lowest possible
pressure.
[Pressure] Enter value Sets a target value for the regulated
pressure.
[Shelf temperature]
Enter value Specifies a temperature for the
shelves.
Editing the settings for a phase
u Tap the phase that you wish to edit.
ð The control panel highlights the selected phase in green.
u Tap the setting that you wish to edit.
u Edit the setting as required.
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u Tap the function [Save] on the function bar.
ð The setting is changed.
7.7 Deleting a method
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the function [Delete] on the function bar.
u Select [Confirm] to confirm the action in response to the confirmation question.
ð The method is deleted.
7.8 Setting end point definitions
The end of a phase can be automatically set for each preparation by defining an end
point.
The end point can be defined by means of the temperature difference test or the pressure difference test.
7.8.1 Connect the sensors for pressure difference test (optional feature)
u Fit the pressure sensor, seal to the connection
and fix with the clamp.
u Plug the pressure sensor electrical connector
into the socket marked Internal Vacuum Sensor .
u Fit the pressure sensor, seal to the connection
and fix with the clamp.
u Plug the pressure sensor electrical connector
into the socket marked External Vacuum
Sensor.
u On the control panel, select the sensor Inficon
Porter CDG020 D on the [Settings] submenu.
7.8.2 Pressure difference test (optional feature)
NOTE
The pressure difference test can only be carried out with a pressure difference test
kit. See Chapter12.1.1 "Accessories", page87
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The pressure difference test establishes the difference between the readings from
two pressure sensors in the drying chamber. If the difference between the two sensor
readings is below a threshold, the freeze-drying phase can be ended.
Navigation path
➔ Method
Precondition:
R A vacuum test has been carried out with a pressure equal to the pressure during
the subsequent freeze-drying process. See Chapter9.2 "Performing a vacuum
test", page79
R The sensors for the pressure difference test have been fitted. See Chapter7.8.1
"Connect the sensors for pressure difference test (optional feature)", page56
R The offset value has been determined. See Chapter7.8.4 "Determining offset
value", page59
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the action [Phase].
ð The control panel shows the action Phase.
u Tap the setting [End point definition].
ð The control panel shows the setting End point definition.
u Tap [Pressure difference test].
ð The control panel shows the pressure difference test.
The following settings are available:
Setting Option Explanation
[Pressure difference test]
Yes/No Switches the pressure differ-
ence test on or off.
[Start time] Enter value Sets the time from which the
pressure difference test is to be
performed. The value relates to
the time before completion of
the primary drying phase.
[Pressure difference limit]
Enter value Specifies the difference between
the two sensor readings below
which the end point is reached.
The threshold must be greater
than the offset value from the
vacuum test. See Chapter7.8.4
"Determining offset value",
page59
[Term] Enter value Specifies the length of time for
which the pressure difference
test is to be carried out.
[Continue] Yes/No Yes : the method switches to the
next phase.
No: the phase is ended when
the set levels are reached.
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Setting Option Explanation
[Message] Yes/No The control panel shows or does
not show a message as soon as
the pressure difference test is
passed.
7.8.3 Temperature difference test
NOTE
The temperature test is successfully completed if all drying shelves are below the
threshold.
The samples on a drying shelf have different drying times. Take account of the different drying times in the [Duration] setting.
The temperature difference test establishes the difference between the readings from
the temperature sensor for the heatable shelf and the temperature sensor in the sample. If the difference between the two sensor readings is below a threshold, the
freeze-drying phase can be ended.
Navigation path
➔ Method
Precondition:
R The heatable shelves are fitted in the rack. See Chapter8 "Operating top-mount
drying racks", page65
R The optional temperature sensor has been installed. See Chapter8 "Operating
top-mount drying racks", page65
u Place the optional temperature sensor in the sample.
u Navigate to the [Method] menu via the navigation path.
u Tap the name of the method that you wish to edit.
ð The control panel highlights the selected method in green.
u Tap the action [Phase].
ð The control panel shows the action Phase.
u Tap the setting [End point definition].
ð The control panel shows the setting End point definition.
u Tap [Temperature difference test].
ð The control panel shows the temperature difference test.
The following settings are available:
Setting Option Explanation
[Temperature
difference test]
Yes/No Switches the temperature differ-
ence test on or off.
[Start time] Enter value Sets the time from which the
temperature difference test is to
be performed. The value relates
to the time before completion of
the primary drying phase.
[Temperature
difference limit]
Enter value Specifies the difference between
the two sensor readings below
which the end point is reached.
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Setting Option Explanation
[Term] Enter value The period of time during which
the difference is not to be exceeded. If the threshold is not
exceeded for the full period of
time, the temperature difference
test is passed.
[Continue] Yes/No Yes : the method switches to the
next phase.
No: the phase is ended when
the set levels are reached.
[Message] Yes/No The control panel shows or does
not show a message as soon as
the temperature difference test
is passed.
7.8.4 Determining offset value
Navigation path
➔ Configuration ➔ End point determination ➔ Pressure difference test
u Navigate to the Pressure Difference Test view via the navigation path.
ð The control unit shows the offset figure.
7.9 Performing freeze-drying using a method [Pro control panel]
7.9.1 Preparing the instrument
Time
required:
NOTE
To minimize the condensation of moisture from the air in the condenser, place a drying rack.
Navigation path
➔ Start
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Start conditioning] on the function bar.
approx.
30min
ð The temperature in the ice condenser decreases to operating temperature.
ð The vacuum pump is brought up to operating temperature.
ð After completion of the conditioning phase, the status bar shows the status
Unload / Load.
7.9.2 Selecting a method
Navigation path
➔ Method
u Navigate to the [Method] menu via the navigation path.
u Tap the function [Method] on the function bar.
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u Tap the method that you wish to use.
u Tap the function [Activate] on the function bar.
ð The status bar shows the method activated.
7.9.3 Starting freeze-drying
CAUTION
Risk of skin burns from touching parts of the ice condenser after completion of conditioning.
u Wear protective gloves when working on the instrument after the conditioning
phase.
NOTE
The freeze-drying process can be cancelled by tapping the functions [Manual] and
[Aerate] on the [Start] menu.
Navigation path
➔ Start
If a gas is being used
Precondition:
R The instrument has been prepared.
R A method is selected.
u Fit a top-mount drying rack. See Chapter8 "Operating top-mount drying racks",
page65.
u Load the top-mount drying rack with frozen samples.
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Start] on the function bar.
u Make sure that the specified gas is being used.
u Answer YES to the confirmation question.
ð The freeze-drying process starts.
ð The control panel shows the S tar t menu with a black background.
ð The system carries out the selected method.
If a gas is not being used
Precondition:
R The instrument has been prepared.
R A method is selected.
u Fit a top-mount drying rack. See Chapter8 "Operating top-mount drying racks",
page65.
u Load the top-mount drying rack with frozen samples.
u Navigate to the [Start] menu via the navigation path.
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u Tap the function [Start] on the function bar.
ð The freeze-drying process starts.
ð The control panel shows the S tar t menu with a black background.
ð The system carries out the selected method.
7.9.4 Ending freeze-drying
Navigation path
➔ Start
Precondition:
R The status bar is showing the status Hold.
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Aerate] on the function bar.
u Answer YES to the confirmation question.
ð The system is vented.
ð The status bar shows the status Aerating.
u Wait until the status bar shows the status Unload / Load.
u Remove the dried preparation from the top-mount drying rack.
7.9.5 Shutting down the instrument
Time
40min
required:
NOTICE
Do not use force to remove the ice from the ice condenser.
Navigation path
➔ Start
Precondition:
R The freeze-drying process has ended.
u Position the condensate drain hose so that the condensate can be drained off.
See Chapter5.3.9 "Preparing the condensate drain hose", page27
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Shut down] on the function bar.
ð The instrument is shutting down.
ð The status bar shows the remaining time and the status Defrosting.
ð After the instrument has shut down, the status bar shows the remaining time
and the status Shutting down.
7.9.6 Switching off the instrument
Precondition:
R The instrument has been shut down. See Chapter7.9.5 "Shutting down the instru-
ment", page61
u Switch the On/Off master switch to Off.
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7.10 Performing freeze-drying manually [Pro control panel]
7.10.1 Preparing the instrument
Time
re-
approx.
30min
quired:
NOTE
To minimize the condensation of moisture from the air in the condenser, place a drying rack.
Navigation path
➔ Start
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Start conditioning] on the function bar.
ð The temperature in the ice condenser decreases to operating temperature.
ð The vacuum pump is brought up to operating temperature.
ð After completion of the conditioning phase, the status bar shows the status
Unload / Load.
7.10.2 Starting freeze-drying
CAUTION
Risk of skin burns from touching parts of the ice condenser after completion of conditioning.
u Wear protective gloves when working on the instrument after the conditioning
phase.
Navigation path
➔ Start
Precondition:
R The instrument has been prepared.
u Fit a top-mount drying rack. See Chapter8 "Operating top-mount drying racks",
page65.
u Load the top-mount drying rack with frozen samples.
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Manual] on the function bar.
u Enter the required settings for the process parameters.
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u Tap the function [Start] on the function bar.
ð The freeze-drying process starts.
ð The background color of the S tar t menu changes from white to black.
ð The status bar shows a clock counting up and the status Manual Drying.
ð The system evacuates to the set pressure.
7.10.3 Editing parameters while the process is running
Navigation path
➔ Start
Precondition:
R The process has been started.
u Navigate to the [Start] menu via the navigation path.
u Using the navigation control, navigate to the parameter that you wish to change.
u Tap the function [Edit] on the function bar.
ð The control panel shows a dialog box with a numeric input box.
ð The control panel highlights the selected parameter in white.
u Enter the value in the numeric input box.
u Tap the function [Save] on the function bar.
ð The setting is saved.
ð The dialog box closes.
7.10.4 Ending freeze-drying
Navigation path
➔ Start
Precondition:
R The sample is dry.
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Aerate] on the function bar.
u Answer YES to the confirmation question.
ð The system is vented.
ð The status bar shows the status Aerating.
u As soon as the status bar shows the status Unload / Load, remove the dried sam-
ple from the drying rack.
7.10.5 Shutting down the instrument
Time
40min
required:
NOTICE
Do not use force to remove the ice from the ice condenser.
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7 | Operating Pro control panel BÜCHI Labortechnik AG
Navigation path
➔ Start
Precondition:
R The freeze-drying process has ended.
u Position the condensate drain hose so that the condensate can be drained off.
See Chapter5.3.9 "Preparing the condensate drain hose", page27
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Shut down] on the function bar.
ð The instrument is shutting down.
ð The status bar shows the remaining time and the status Defrosting.
ð After the instrument has shut down, the status bar shows the remaining time
and the status Shutting down.
7.10.6 Switching off the instrument
Precondition:
R The instrument has been shut down. See Chapter7.10.5 "Shutting down the in-
strument", page63
u Switch the On/Off master switch to Off.
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8 Operating top-mount drying racks
8.1 Operating stoppering acrylic drying chamber
u Make sure that the groove above the ice con-
denser is clean, free of dust and not
scratched.
u Check the 300mm dia. O-ring for damage and
grease it with vacuum grease.
u Place the 300mm dia. O-ring in the groove
above the ice condenser.
u Locate the springs in the holes on the ice con-
denser.
u Place the intermediate plate on the ice con-
denser.
u Loosen all fixing screws.
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8 | Operating top-mount drying racks BÜCHI Labortechnik AG
u Align the slots for the shelves.
u Tighten the fixing screws.
u Place the rack on the intermediate plate.
u Slide the shelves into the rack.
u Press the plug onto the shelf connection and
turn the ring counterclockwise at the same
time.
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u Locate the cylinder in the groove above the
main plate.
u Check the O-ring for damage and grease it
with vacuum grease.
u Screw the hand wheel upwards until the plate
inside the lid has pressed the hooks all the
way in.
u Place the O-ring into the groove of the cover.
u Place the cover on the cylinder.
u Make sure that the hooks on the rack are en-
gaged.
u Carry out freeze-drying.
Precondition:
R The status bar shows the status Stoppering.
u Turn the hand wheel, until all samples are
sealed.
u Confirm the verification question on the control
panel.
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8 | Operating top-mount drying racks BÜCHI Labortechnik AG
8.2 Operating manifold acrylic drying chamber (heatable shelves)
u Make sure that the groove above the ice con-
denser is clean, free of dust and not
scratched.
u Check the 300mm dia. O-ring for damage and
grease it with vacuum grease.
u Place the 300mm dia. O-ring in the groove
above the ice condenser.
u Place the intermediate plate on the ice con-
denser.
u Loosen all fixing screws.
u Align the slots for the shelves.
u Tighten the fixing screws.
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u Place the rack on the intermediate plate.
u Slide the shelves into the rack.
u Press the plug onto the shelf connection and
turn the ring counterclockwise at the same
time.
(option)
u Turn the temperature sensor electrical con-
nector so that the marks on the connector and
the heatable shelf are parallel
u Press the temperature sensor electrical con-
nector onto the connection.
u Locate the cylinder in the groove above the
main plate.
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8 | Operating top-mount drying racks BÜCHI Labortechnik AG
u Fit manifold valve onto the connection on the
top-mount drying rack.
u Check the O-ring for damage and grease it
with vacuum grease.
u Place the O-ring into the groove of the cover.
u Place the manifold cover on the cylinder.
u Carry out freeze-drying.
8.3 Operating manifold acrylic drying chamber (non-heatable shelf)
u Make sure that the groove above the ice con-
denser is clean, free of dust and not
scratched.
u Check the 300mm dia. O-ring for damage and
grease it with vacuum grease.
u Place the 300mm dia. O-ring in the groove
above the ice condenser.
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u Place the intermediate plate on the ice con-
denser.
u Place the rack on the intermediate plate.
u Locate the cylinder in the groove above the
main plate.
u Fit manifold valve onto the connection on the
top-mount drying rack.
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8 | Operating top-mount drying racks BÜCHI Labortechnik AG
u Check the O-ring for damage and grease it
with vacuum grease.
u Place the O-ring into the groove of the cover.
u Place the manifold cover on the cylinder.
u Carry out freeze-drying.
8.4 Operating acrylic drying chamber (heatable shelf)
u Make sure that the groove above the ice con-
denser is clean, free of dust and not
scratched.
u Check the 300mm dia. O-ring for damage and
grease it with vacuum grease.
u Place the 300mm dia. O-ring in the groove
above the ice condenser.
u Place the intermediate plate on the ice con-
denser.
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u Loosen all fixing screws.
u Align the slots for the shelves.
u Tighten the fixing screws.
u Place the rack on the intermediate plate.
u Slide the shelves into the rack.
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u Press the plug onto the shelf connection and
turn the ring counterclockwise at the same
time.
(option)
u Turn the temperature sensor electrical con-
nector so that the marks on the connector and
the heatable shelf are parallel
u Press the temperature sensor electrical con-
nector onto the connection.
u Locate the cylinder in the groove above the
main plate.
u Check the O-ring for damage and grease it
with vacuum grease.
u Place the O-ring into the groove of the cover.
u Place the cover on the cylinder.
u Carry out freeze-drying.
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8.5 Operating acrylic drying chamber (on-heatable shelf)
u Make sure that the groove above the ice con-
denser is clean, free of dust and not
scratched.
u Check the 300mm dia. O-ring for damage and
grease it with vacuum grease.
u Place the 300mm dia. O-ring in the groove
above the ice condenser.
u Place the intermediate plate on the ice con-
denser.
u Place the rack on the intermediate plate.
u Locate the cylinder in the groove above the
main plate.
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u Check the O-ring for damage and grease it
with vacuum grease.
u Place the O-ring into the groove of the cover.
u Place the cover on the cylinder.
u Carry out freeze-drying.
8.6 Operating manifold drying rack
u Make sure that the groove above the ice con-
denser is clean, free of dust and not
scratched.
u Check the 300mm dia. O-ring for damage and
grease it with vacuum grease.
u Place the 300mm dia. O-ring in the groove
above the ice condenser.
u Fit manifold valve onto the connection on the
top-mount drying rack.
u Place the base plate on the ice condenser.
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1
2
A
B
u Place the seal on the connection.
u Fit the manifold drying rack onto the seal and
fix it with the clamp.
u Carry out freeze-drying.
8.7 Operating manifold valves
The lever on a manifold valve can be set to the following positions:
Position Function
A: Lever pointing up The connected vessel is evacuated.
B: Lever pointing down The connected vessel is vented.
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9 | Cleaning and servicing BÜCHI Labortechnik AG
9 Cleaning and servicing
NOTE
Users may only carry out the servicing and cleaning operations described in this section.
Any servicing and repair work which involves opening up the casing may only be carried out by BUCHI service technicians.
9.1 Regular maintenance work
Component Action Interval
Vacuum Pump u Carry out maintenance according to
the manufacturer's instructions. See
relevant documentation.
See manufacturer's
instructions
Top-mount drying
racks
O-ring, dia.
300mm
u Wipe down the top-mount drying
racks with a damp cloth.
u If heavily soiled, use a mild detergent.
u Wipe down the 300mm O-rings with
a damp cloth.
u Check for scratches and other dam-
age.
u Replace the 300mm O-ring if it is
damaged.
u Grease with vacuum grease if neces-
sary.
u Replace the 300mm O-ring. Annually
Seals, ISO-KF 16 u Wipe down the seals with a damp
Seals, ISO-KF 25
cloth.
u Check for scratches and other dam-
age.
u Replace seals if they are damaged.
Vacuum tubing u Wipe down the vacuum tube with a
damp cloth.
u Check for scratches and other dam-
age.
u Replace the vacuum tube if it is dam-
aged.
Daily
Daily
Annually
Annually
Ice condenser u Rinse out with water.
u If heavily soiled, use ethanol or a mild
Daily
detergent.
Drain valve strainer u Clean the strainer. Daily
Casing u Wipe down the casing with a damp
Weekly
cloth.
u If heavily soiled, use ethanol or a mild
detergent.
Warning symbols u Check that the warning symbols on
Weekly
the instrument are legible.
u If they are dirty, clean them.
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BÜCHI Labortechnik AG Cleaning and servicing | 9
Component Action Interval
Draining valve u Remove the strainer.
u Fill ice condenser with water.
u Open the drain valve manually.
u Loosen deposits from drain valve us-
Weekly
ing compressed air.
Heat exchanger u Remove dust and foreign objects
from the ventilation slots using compressed air or a vacuum cleaner.
View u Wipe down the display with a damp
cloth.
9.2 Performing a vacuum test
The vacuum test checks the performance capacity of the vacuum system.
Time
required:
Navigation path
➜ Configuration ➜ Servicing ➜ Vacuum test
Precondition:
R Conditioning of the instrument has been completed.
R A top-mount drying rack is fitted.
R The top-mount drying rack does not contain sample.
u Navigate to the action [Vacuum Test] via the navigation path.
u Specify a required setting for the vacuum to be achieved.
u Specify a required time within which the vacuum is to be reached.
u Tap the function [Start] on the function bar.
max. 10 min
ð The vacuum test starts.
Monthly
Monthly
ð The status bar shows the status Vacuum Test.
ð If the vacuum pressure is not below 500 mbar after 30 s, the vacuum test au-
tomatically aborts.
ð After completion of the vacuum test, the [Vacuum test] line shows whether the
vacuum test has been passed or not.
Troubleshooting after failed vacuum test
Possible cause Action
Top-mount drying rack not correctly fitted Fit the top-mount drying rack correctly.
Top-mount drying rack is damaged Check function of PMMA parts, replace
manifold valves, clean drain valve.
O-rings dirty Wipe down the O-rings with a damp
cloth.
O-rings damaged Check O-rings and replace if necessary.
Groove of O-rings dirty Wipe groove of O-rings with a damp
cloth.
KF clamps not closed Close the KF clamps.
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9 | Cleaning and servicing BÜCHI Labortechnik AG
Possible cause Action
KF seals dirty Wipe down the KF seals with a damp
cloth.
KF seals damaged Inspect KF seals and replace as neces-
sary.
The pump connected is not delivering
sufficient performance
Carry out vacuum test with a different
vacuum pump.
9.3 Performing a leak test
9.3.1 Performing a leak test with a drying chamber
The leak test checks the vacuum system for possible leaks.
Time
required:
NOTE
The leak test can be aborted by tapping [Stop] on the function bar.
Precondition:
R Conditioning of the instrument has been completed.
R A top-mount drying rack is fitted.
R The top-mount drying rack does not contain sample.
u Navigate to the action [Leak test] via the navigation path.
u On the [Ice Condenser] line, enter a required setting for the vacuum.
u On the [Shelf temperature] line enter the required shelf temperature.
u On the [Test scope] line select the component to be tested.
u On the [Drying shelf heating] line switch the drying shelf heating on or off.
u On the [Volume] line enter the actual volume of the components to be tested.
45 min
The volume of the system is calculated from the volume of the ice condenser and the
top-mount drying rack.
36.46L
Acrylic drying chamber
(with 4 drying shelves)
u Tap the function [Start] on the function bar.
43.41L
Acrylic drying chamber
(with 6 drying shelves)
ð The leak test starts.
ð The status bar shows the status Leak Test.
ð After completion of the leak test, the [Leak test] line shows whether the leak test
has been passed or not.
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ð The leak test is passed if the measured leakage rate is less than the pre-set rate
of 10.10mbar*L/h.
Troubleshooting after failed leak test
Possible cause Action
Top-mount drying rack not correctly fitted Fit the top-mount drying rack correctly.
O-rings dirty Wipe down the O-ring with a damp cloth.
O-rings damaged Check O-rings and replace if necessary.
Groove of O-rings dirty Wipe groove of O-rings with a damp
cloth.
KF clamps not closed Close the KF clamps.
KF seals dirty Wipe down the KF seals with a damp
cloth.
KF seals damaged Inspect KF seals and replace as neces-
sary.
Pump oil dirty Service according to manufacturer's in-
structions.
Leaking manifold valves
(manifold acrylic drying chamber only)
Replace leaking manifold valve.
Locate leaking manifold valve. See
Chapter10.3 "Locating leaking manifold
valve", page84
9.3.2 Performing leak test with a manifold drying rack
The leak test checks the vacuum system for possible leaks.
Time
45 min
required:
NOTE
The leak test can be aborted by tapping [Stop] on the function bar.
Precondition:
R Conditioning of the instrument has been completed.
u Fit the base plate with a KF 40 blind flange, an ISO-KF 40 seal and an ISO-KF 40
clamp.
u Navigate to the action [Leak test] via the navigation path.
u On the [Ice Condenser] line, enter a required setting for the vacuum.
u On the [Shelf temperature] line enter the required shelf temperature.
u On the [Test scope] line select the component to be tested.
u On the [Drying shelf heating] line switch the drying shelf heating on or off.
u On the [Volume] line enter the actual volume of the components to be tested.
The volume of the system is calculated from the volume of the ice condenser and the
top-mount drying rack.
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9 | Cleaning and servicing BÜCHI Labortechnik AG
13.64L
u Tap the function [Start] on the function bar.
ð The leak test starts.
ð The status bar shows the status Leak Test.
ð When the leak test is completed, the [Leak test] tab indicates whether the leak test
was successful.
ð The leak test is passed if the measured leakage rate is less than the pre-set rate
of 10.10mbar*L/h.
NOTE
To test the leak-tightness of the manifold drying rack, carry out another leak test with
the manifold drying rack installed.
Troubleshooting after failed leak test
Possible cause Action
Top-mount drying rack not correctly fitted Fit the top-mount drying rack correctly.
O-rings dirty Wipe down the O-ring with a damp cloth.
O-rings damaged Check O-rings and replace if necessary.
Groove of O-rings dirty Wipe groove of O-rings with a damp
cloth.
KF clamps not closed Close the KF clamps.
KF seals dirty Wipe down the KF seals with a damp
cloth.
KF seals damaged Inspect KF seals and replace as neces-
sary.
Pump oil dirty Service according to manufacturer's in-
structions.
Leaking manifold valves
(only if manifold drying rack installed)
Replace leaking manifold valve.
Locate leaking manifold valve. See
Chapter10.3 "Locating leaking manifold
valve", page84
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BÜCHI Labortechnik AG Help with faults | 10
10 Help with faults
10.1 Troubleshooting
Problem Possible cause Action
Compressor fails to
start or switches off
No power supply to compressor
Compressor overheated u Allow the compressor to
Circuit-breaker has tripped u Reset circuit of L-200.
u Check the power supply
and switch on the device.
cool down.
u Check ambient conditions.
Cooling temperature is not achieved
Wrong ambient conditions Adjust ambient conditions.
Too much ice in the ice condenser
10.2 Faults, possible causes and remedies
Malfunction Possible cause Remedy
Instrument does
not work
Main valve or vacuum pump
switches over frequently
Main valve does
not switch over
Instrument is not connected to
the power supply.
Vacuum system is leaking. u If necessary, replace tub-
Main valve fitted wrong way
round.
Main valve lead not plugged
in.
See Chapter5.1 "Installation
site", page21
Defrost the ice condenser.
u Make sure that the power
supply is connected and
switched on.
ing and/or seals.
u Fit main valve correct way
round (arrow pointing towards vacuum pump).
u Make sure that the electri-
cal lead of the main valve
is plugged into the correct
socket on the rear of the
instrument.
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10 | Help with faults BÜCHI Labortechnik AG
Malfunction Possible cause Remedy
Vacuum is not
achieved
Vacuum system is leaking. u Clean the drain valve.
u Check manifold valve. See
Chapter10.3 "Locating
leaking manifold valve",
page84
u Check regulating valve and
aeration valve. See Chapter10.4 "Finding a leaking
regulating valve or aeration
valve", page84
u If necessary, replace tub-
ing and/or seals.
Vacuum pump is too weak. u Use a vacuum pump with a
capacity of at least 5m3/h.
u Carry out maintenance
work according to the vacuum pump manufacturer's
documentation.
Instrument is not
vented
Main valve is incorrectly connected.
10.3 Locating leaking manifold valve
Navigation path
➔ Start
Precondition:
R The system does not evacuate to less than 0.1 mbar.
u Navigate to the Sta rt view via the navigation path.
u Set the vacuum to 0.5 mbar.
u Tap the function [Start] on the function bar.
u Turn each manifold valve individually while checking the display to see if the ac-
tual pressure changes.
u If the vacuum decreases in the case of one of the manifold valves, then that valve
is the one that is leaking.
u Tap the function [Aerate] on the function bar.
u Replace the manifold valve concerned.
u Connect the main valve
properly (see Chapter5.3.3 "Fitting the advanced vacuum control
unit", page23).
10.4 Finding a leaking regulating valve or aeration valve
Navigation path
➔ Start
Precondition:
R The instrument has been prepared.
u Remove regulating or aeration valve.
u Seal the opening with KF16 cover.
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u Fit a top-mount drying rack. See Chapter8 "Operating top-mount drying racks",
page65.
u Navigate to the [Start] menu via the navigation path.
u Tap the function [Manual] on the function bar.
u Enter the specified settings for the vacuum.
u Tap the function [Start] on the function bar.
ð The background color of the S tar t menu changes from white to black.
ð The status bar shows a clock counting up and the status Manual Drying.
ð The system evacuates to the set pressure.
ð If the specified setting is not reached, the valve is leaking.
u Carry out the instructions for action on the other valve.
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11 | Taking out of service and disposal BÜCHI Labortechnik AG
11 Taking out of service and disposal
11.1 Disposal
The operator is responsible for proper disposal of the Lyovapor™.
CAUTION
Potential environmental hazard
The device uses refrigerant R507. This refrigerant is toxic and must not be allowed to
enter the soil or groundwater.
u Dispose of the appliance properly, if necessary using a professional disposal ser-
vice.
u When disposing of equipment observe the local regulations and statutory require-
ments regarding waste disposal.
11.2 Returning the instrument
Before returning the instrument, contact the BÜCHI Labortechnik AG Service Department.
https://www.buchi.com/contact
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BÜCHI Labortechnik AG Appendix | 12
12 Appendix
12.1 Spare parts and accessories
Use only genuine BUCHI consumables and spare parts in order to ensure correct,
safe and reliable operation of the system.
NOTE
Any modifications of spare parts or assemblies are only allowed with the prior written
permission of BUCHI.
12.1.1 Accessories
Description Order no. Image
Main valve 11062223
Pressure regulation valve 11064725
Aeration valve 11064724
Pirani / Piezo pressure sensor PPG010 11062228
Capacitive pressure sensor Inficon CDG
020 D
PT1000 sample temperature sensor 11064031
11066564
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12 | Appendix BÜCHI Labortechnik AG
Description Order no. Image
Set for pressure difference test at L-200
incl. PPG010 and CDG 020D sensors, PMMA
top cover, seals and clamps
11067590
12.1.2 Further accessories
Description Order no. Image
Trolley L-200, stainless steel coated 11063692
SD-Card 1 GB 11064730
Connection cable, for vacuum pump 11064934
12.1.3 Spare parts
Description Order no. Image
User interface 11063580
User interface Pro 11063581
O-Ring Ø 300 mm 11065367
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BÜCHI Labortechnik AG Appendix | 12
Description Order no. Image
Vacuum hose, KF 25, L 1000 mm 11066031
Vacuum clamp KF 16 11064939
Vacuum clamp KF 25 11063662
Vacuum clamp, KF 40 11063663
Vacuum seal KF 16 11063455
Vacuum seal KF 25 11063457
Vacuum seal KF 40 11063659
Vacuum flange adapter, stainless steel,
11064870
KF 16 to KF 25
Blind flange KF 16 11064902
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12 | Appendix BÜCHI Labortechnik AG
Description Order no. Image
Blind flange KF 25 11063660
Blind flange KF 40 11063661
12.1.4 Top-mount drying rack accessories
Description Order no. Image
Drying chamber tube, PMMA, for 4
11063278
shelves
L 368 mm, Ø 300 mm
Drying chamber tube, PMMA, for 6
shelves
L 480 mm, Ø 300 mm
Top cover, PMMA, without sealing
Ø 300 mm, H 50 mm
Top cover manifold, PMMA, with 12
valves, without sealing
Ø 300 mm, W 546 mm, H 127 mm
Top cover stoppering, without sealing
only for rack for 4 heatable shelves
Ø 300 mm, H 330 mm, W 320 mm
11065093
11062912
11065595
11064314
Rack for 4 heatable shelves
H 356 mm, Ø 265 mm , shelf distance 30-75
mm
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BÜCHI Labortechnik AG Appendix | 12
Description Order no. Image
Rack for 6 heatable shelves
H 468 mm, Ø 265 mm, shelf distance 30-75
mm
11065103
Heatable shelf, aluminum coated, with
connection cable
Ø 219.5 mm, surface area 376 cm²
Sample tray, stainless steel
Ø 220 mm, H 18.5 mm
Ferrule,
Ø 218 mm, H 40 mm
Drying rack manifold, stainless steel,
with 12 valves
H 340 mm, W 777 mm
11064095
11061439
11065816
11063664
Drip pan, for manifold 11066358
Support for rack, stainless steel
H 4 mm
Baseplate, stainless steel, for manifold
11063789
11064953
rack
Baseplate, PMMA, for manifold rack 11065733
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12 | Appendix BÜCHI Labortechnik AG
Description Order no. Image
Suction nipple
For manifold application to create vacuum in
sample flasks
11065819
Ampoule adapter for manifold
with 19 ampoule connections and cap
adapter
Manifold valve, natural ruber, with SJ
29/32
Flask beaker for manifold 100 mL
with cap adapter and integrated filter
Flask beaker for manifold 200 mL
with cap adapter and integrated filter
11065725
11062300
11066140
11066141
Flask beaker for manifold 600 mL
with cap adapter and integrated filter
Flask beaker for manifold 800 mL
with cap adapter and integrated filter
Flask beaker for manifold 1200 mL
with cap adapter and integrated filter
Manifold flask adapter set
with 12 adapters, incl. filter paper
11066142
11069474
11066143
11066144
92/94 Operation Manual Lyovapor™ L-200/L-200 Pro
Page 93
BÜCHI Labortechnik AG Appendix | 12
Description Order no. Image
Manifold flask adapter set
with 6 adapters, incl. filter paper
11067334
Manifold flask adapter set, US joint size
with 12 adapters, incl. filter paper
Manifold flask adapter set, US joint size
with 6 adapters, incl. filter paper
12.1.5 Software
Lyovapor™ software licence 11065668
Lyovapor™ software DVD 11065667
11066171
11067333
Order no.
Operation Manual Lyovapor™ L-200/L-200 Pro 93/94
Page 94
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