Mindray BC 3000Plus Service Manual

BC-3000 Plus
Auto
Hematology
Analyzer

Service Manual

Copyright

®

SHENZHEN MINDRAY

Statement

SHENZHEN MINDRAY
Mindray) owns all rights to this unpublished work and intends to maintain this work as
confidential. Mindray may also seek to maintain this work as an unpublished copyright.
This publication is to be used solely for the purposes of reference, operation, maintenance,
or repair of Mindray equipment. No part of this can be disseminated for other purposes.

BIO-MEDICAL ELECTRONICS CO., LTD. 2003

®

BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called

In the event of inadvertent or deliberate publication, Mindray intends to enforce its rights to
this work under copyright laws as a published work. Those having access to this work may
not copy, use, or disclose the information in this work unless expressly authorized by
Mindray to do so.

All information contained in this publication is believed to be correct. Mindray shall not be
liable for errors contained herein nor for incidental or consequential damages in
connection with the furnishing, performance, or use of this material. This publication may
refer to information and protected by copyrights or patents and does not convey any
license under the patent rights of Mindray, nor the rights of others. Mindray does not
assume any liability arising out of any infringements of patents or other rights of third
parties.

Content of this manual is subject to changes without prior notice.

PROPERTY OF

®

SHENZHEN MINDRAY

BIO-MEDICAL ELECTRONICS CO., LTD.

ALL RIGHTS RESERVED

Responsibility on the manufacturer party

Mindray is responsible for safety, reliability and performance of this equipment only in the
condition that:

Auto Hematology Analyzer Service Manual (V1.1) i

•

all installation, expansion, change, modification and repair of this equipment are
conducted by Mindray qualified personnel;

•

applied electrical appliance is in compliance with relevant National Standards;

•

the instrument is operated under strict observance of this manual.

Note

This equipment is not intended for family usage.
This equipment must be operated by skilled/trained clinical personnel.

Warning

It is important for the hospital or organization that employs this equipment to carry out a
reasonable maintenance schedule. Neglect of this may result in machine breakdown or
injury of human health.

Upon request, Mindray may provide, with compensation, necessary circuit diagrams,
calibration illustration list and other information to help qualified technician to maintain and
repair some parts, which Mindray may define as user serviceable.

ii Auto Hematology Analyzer Service Manual (V1.1)

Warranty

Workmanship & Materials

Mindray guarantees new equipment other than accessories to be free from defects in
workmanship and materials for a period of one year from date of shipment under normal
use and service. Mindray's obligation under this warranty is limited to repairing, at
Mindray’s option, any part which upon Mindray's examination proves defective.

THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,
EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANT ABILITY OR
FITNESS FOR ANY PARTICULAR PURPOSE.

Exemptions

Mindray's obligation or liability under this warranty does not include any transportation or
other charges or liability for direct, indirect or consequential damages or delay resulting
from the improper use or application of the product or the substitution upon it of parts or
accessories not approved by Mindray or repaired by anyone other than a Mindray
authorized representative.

This warranty shall not extend to any instrument which has been subjected to misuse,
negligence or accident; any instrument from which Mindray's original serial number tag or
product identification markings have been altered or removed, or any product of any other
manufacturer.

Safety, Reliability and Performance

Mindray is not responsible for the effects on safety, reliability and performance of the
BC-3000plus Hematology Analyzer if:

■ assembly operations, extensions, re-adjusts, modifications or repairs are carried out
by persons other than those authorized by Mindray.

■ Personnel unauthorized by Mindray repairs or modifies the instrument.

Auto Hematology Analyzer Service Manual (V1.1) iii

Return Policy

Return Procedure

In the event that it becomes necessary to return a unit to Mindray, the following procedure
should be followed:

1. Obtain return authorization. Contact the Mindray Service Department and obtain a
Customer Service Authorization (Mindray) number . The Mindray number must appear
on the outside of the shipping container. Return shipments will not be accepted if the
Mindray number is not clearly visible. Please provide the model number, serial
number, and a brief description of the reason for return.

2. Freight policy. The customer is responsible for freight charges when equipment is
shipped to Mindray for service (this includes customs charges).

Company Contact

Address: Mindray Building, Keji 12th Road South, Hi-tech

Industrial Park, Nanshan, Shenzhen, P. R. China

Phone: [phone]
Fax: [phone]

Authorized Representative

Name: Shanghai International Holding Corp. GmbH (Europe)

Address: Eiffestrasse 80 D-20537 Hamburg Germany

Phone: [phone]
Fax: [phone]

iv Auto Hematology Analyzer Service Manual (V1.1)

Conventions Used in This Manual and Instrument

Warnings, Cautions and Notes

Warnings, cautions and notes are used in this manual to alert or signal the reader to
specific information.

WARNING

Warning alerts the user to the possible injury or death associated with the use or
misuse of the instrument.

CAUTION

Caution alerts the user to possible injury or problems with the instrument
associated with its use or problem such as instrument malfunction, instrument
failure, damage to the instrument.

NOTE

Note provides specific information, in the form of recommendations,
pre-requirements, alternative goods or supplemental information.

WARNING

Potential biohazard

Avoid contacting with the sample probe.

WARNING

Auto Hematology Analyzer Service Manual (V1.1) i

Content

Content

Chapter 1 General………………………………………………...……………………………1-1

1.1 Introduction ..............................................................................................................1-1

1.2 Service Policy...........................................................................................................1-2

1.3 Specification.............................................................................................................1-3

1.4 Panel Description.....................................................................................................1-7

1.4.1 Front Panel and Keys............................................................................................1-7

1.4.2 Rear Panel............................................................................................................1-9

1.4.3 Front review without front panel.......................................................................... 1-11

1.4.4 Right-side view without the door .........................................................................1-12

1.4.5 Left-side view without the door............................................................................ 1-13

1.5 Menu Structure Chart.............................................................................................1-14

Chapter 2 Troubleshooting…………………………………………………………………….2-1

2.1 Check Procedure .....................................................................................................2-1

2.2 Check Items before Instrument Check.....................................................................2-2

2.3 How to Check Sample Data.....................................................................................2-4

2.4 Troubleshooting Erroneous Data............................................................................2-16

2.5 Troubleshooting......................................................................................................2-21

2.6 Alarm......................................................................................................................2-27

Chapter 3 Hardware……………………………………………………………………………3-1

3.1 CPU Board...............................................................................................................3-2

3.2 Analog Signal Board.................................................................................................3-6

3.3 Power Drive Board.................................................................................................3-10

3.4 Keypad...................................................................................................................3-13

3.5 Recorder Board...................................................................................................... 3-14

3.6 Volumetric Metering Board.....................................................................................3-16

3.7 Power Supply Board ..............................................................................................3-18

Chapter 4 Hydraulic System…………………………………………………………………..4-1

4.1 Hydraulic System Block Diagram.............................................................................4-1

4.2 Units of Hydraulic System........................................................................................4-2

4.3 Whole Blood Count Cycle........................................................................................ 4-3

4.4 Flow Charts of Main Procedures..............................................................................4-4

4.4.1 Power on...............................................................................................................4-4

4.4.2 Whole Blood Count ...............................................................................................4-5

4.4.3 Prediluted Count ................................................................................................... 4-6

4.4.4 Startup...................................................................................................................4-7

4.4.5 Flush Apertures.....................................................................................................4-8

4.4.6 Dispense Diluent...................................................................................................4-9

4.4.7 Shut Down ..........................................................................................................4-10

Auto Hematology Analyzer Service Manual (V1.1) 1

Content

4.5 Hydraulic System Flow Diagram............................................................................ 4-11

4.5.1 Inspire Sample and Diluent (Whole Blood Mode)................................................ 4-11

4.5.2 Inspire Sample and Diluent (Prediluted Mode) .................................................... 4-12

4.5.3 WBC Injection & Lyse Preparation......................................................................4-13

4.5.4 RBC & Lyse Injection..........................................................................................4-14

4.5.5 Mixture ................................................................................................................4-15

4.5.6 Count Cycle.........................................................................................................4-16

4.5.7 Cleaning..............................................................................................................4-17

4.5.8 Flush...................................................................................................................4-18

4.5.9 Empty Tube.........................................................................................................4-19

Chapter 5 System Structure…………………………………………………………………..5-1

5.1 Disassemble/Replace Parts and Components.........................................................5-1

5.1.1 Disassemble Syringe and Replace Piston ............................................................5-1

5.1.2 Replace Sample Probe .........................................................................................5-7

5.1.3 Replace Sample Probe Wipe Block ....................................................................5-12

5.1.4 Replace Vacuum Chamber.................................................................................5-18

5.1.5 Replace Pump.....................................................................................................5-19

5.1.6 Replace Count Bath............................................................................................5-22

5.1.7 Clean or Replace V11 or V12 Valve....................................................................5-26

5.1.8 Replace V11 or V12 ............................................................................................5-27

5.1.9 Clean V11 or V12................................................................................................5-31

5.1.10 Replace TFT Screen.........................................................................................5-33

5.1.11 Replace Recorder Paper...................................................................................5-35

5.1.12 Replace Recorder Module ................................................................................ 5-37

5.2 Disassemble/assemble Circuit Boards...................................................................5-38

5.3 Connect Power Supply...........................................................................................5-39

5.4 Connect Circuit Boards..........................................................................................5-41

5.4.1 CPU board connectors defined...........................................................................5-43

5.4.2 Power Driver Board Connectors Defined ............................................................ 5-48

5.4.3 Analog Board’s connectors defined.....................................................................5-52

5.4.4 Keyboard Connectors defined.............................................................................5-54

5.4.5 Indicator Board Connector Defined.....................................................................5-55

5.4.6 MTB Connector Defined......................................................................................5-55

Chapter 6 Adjustment………………………………………………………………………….6-1

6.1 General....................................................................................................................6-1

6.2 Adjusted procedures ................................................................................................6-1

6.3 Gain of WBC (whole blood and prediluted) Channel................................................6-4

6.4 Gain of RBC Channel............................................................................................... 6-6

6.5 Gain of PLT Channel.............................................................................................. 6-10

6.6 Gain of HGB Channel ............................................................................................ 6-11

6.7 Adjust Display Brightness.......................................................................................6-12

6.8 Adjust Vacuum and Pressure.................................................................................6-13

2 Auto Hematology Analyzer Service Manual (V1.1)

Content

6.9 Adjust Count time...................................................................................................6-14

6.10 Adjust Auto Clean Time........................................................................................6-16

6.1 1 Adjust Volumetric Metering Board ........................................................................6-17

6.12 Re-calibrating Instrument.....................................................................................6-19

Chapter 7 Maintenance………………………………………………………………………..7-1

7.1 Daily maintenance....................................................................................................7-1

7.2 Monthly maintenance...............................................................................................7-1

7.3Half-year maintenance..............................................................................................7-2

Chapter 8 Spare Part List…………..………………………………………………………….8-1

Chapter 9 Performance Test…………………………………………………………………..9-1

Chapter 10 Histograms and Pulse Graphs…………………………………………………10-1

10.1 Histograms...........................................................................................................10-1

10.2 Pulse Graphs .......................................................................................................10-4

10.2.1 Normal Pulse Graphs........................................................................................ 10-4

10.2.2 Abnormal Pulse Graphs .................................................................................... 10-6

Chapter 11 Password and Upgrade software………………………………………………11-1

11.1 Password.............................................................................................................. 11-1

11.2 Upgrade System Software.................................................................................... 11-2

Appendix………………………………………………………………………………………..A-1

Hardware Diagram of BC-3000PLUS.............................................................................A-1

Hydraulic Diagram of BC-3000PLUS .............................................................................A-2

Auto Hematology Analyzer Service Manual (V1.1) 3

General

Chapter 1 General

1.1 Introduction

CAUTION

To maintain the instrument in normal condition, the user must perform the periodic
maintenance. Refer to the user manual.

This service manual provides useful information to help service personnel to understand,
troubleshoot, service, maintain and repair the Hematology Analyzer.

All replaceable parts or units of this instrument and its optional units are clearly list with
exploded illustration to help you locate the parts quickly.

The maintenance must be periodically performed because the instrument has fluid paths
and precision parts. Accordingly, the user is responsible for performing the periodic
maintenance. The “maintenance” chapter in this service manual describes the
maintenance that should be performed by the qualified service personnel. The
“maintenance” chapter in the user manual describes the maintenance that can be
performed by the user.

NOTE

If the instrument has a problem and there has been no periodic maintenance, the
instrument will usually be normal again by cleaning the fluid paths or replacing a
consumable with a new one.

The information in the user manual is primarily for the user. However, it is important for
service personnel to thoroughly read the user manual and service manual before starting
to troubleshoot, service, maintain or repair this instrument. This is because service
personnel needs to understand the operation of the instrument in order to effectively use
the instrument in order to effectively use the information in the service manual.

Auto Hematology Analyzer Service Manual (V1.1) 1-1

General

1.2 Service Policy

CAUTION

Be careful not to directly touch any place where blood is or may spread to.
Wear rubber gloves to protect yourself from infection before doing maintenance.

Our company’s basic policy for technical service is to replace faulty units, printed circuit
boards or parts. We do not support component-level repair of boards and units outside the
factory.

NOTE

When ordering parts or accessories from your nearest distributor, please quote the
part number and part name which is listed in the service manual, and the name or
model of the unit in which the required part is located. This will help us to promptly
attend to your needs.
Always use parts and accessories recommended or supplied by our company to
assure maximum performance from your instrument.

1-2 Auto Hematology Analyzer Service Manual (V1.1)

1.3 Specification

Hemoglobin Analysis

Wavelength 525nm

Sampling Features

Volumes Required for Each Analysis：
Whole Blood Mode (vein blood) 13uL

Prediluted Mode (capillary blood) 20uL

Aspirated volumes：
500uL of lyse first dilution per cycle for WBC measurement
300uL of second dilution per cycle for RBC and PLT measurement

Dilution Ratios Whole Blood Prediluted

General

WBC/HGB 1: 308 1:407

RBC/PLT 1:44833 1: 44274

Cell Counting Aperture Size：
WBC 100um
RBC 70um

Throughput more than 60 samples/hour

Check Diluent, Rinse and Lyse

The applied volume of each reagent is:

Diluent Rinse Lyse E-Z

Normal Startup 42ml 10ml

Prepare a sample

25.4ml 6ml 0.5ml

(whole blood)

Prepare a sample

25.1ml 6ml 0.26ml

(prediluted)

Normal Shutdown 32ml 10ml 1.6ml

Performance Specifications

Imprecision
Imprecision is based on replicate determinations of the same sample. The first

Auto Hematology Analyzer Service Manual (V1.1) 1-3

General

result is not used in the calculation.

Imprecision Specifications

Parameter Level Units CV%

WBC 7.0-15.0 109/L

RBC 3.5-6.0 1012/L
HGB 110 – 180 g/L

MCV 80.0 – 110.0 fL

PLT 200 – 500 109/L

Operating Range

Parameter Range Units

WBC 0.0-999.9 109/L

RBC 0.00-9.99 1012/L

HGB 0-300 g/L

MCV 0-250 fL

PLT 0-3000 109/L

≤ 2.5

≤ 2
≤ 1.5
≤ 0.5

≤ 5

Linearity

Parameter Linearity Range Units

WBC 0.3-99.9 109/L ±0.3 or ±5%

RBC 0.20-9.99 1012/L ±0.05 or ±5%
HGB 0-300 g/L ±2 or ±3%

PLT 10-999 109/L ±10 or ±10%

Display
Liquid Crystal Display（LCD），resolution: 640×480

Input/Output
Two RS232/C serial ports
One printer port
One keyboard interface

Difference

(whichever is greater)

Built-in Thermal Recorder

1-4 Auto Hematology Analyzer Service Manual (V1.1)

Printer (optional)
EPSON LX-300, EPSON LX-300+

Scanner(optional)

TYSSO CCD-82

Reagents Required

DILUENT M-30D DILUENT
RINSE M-30R RINSE
LYSE M-30L LYSE
E-Z CLEANSER（Enzyme cleanser） M-30E CLEANSER
PROBE CLEANSER M-30P CLEANSER

Power
Input: AC 220V±10% AC 110V±10%

General

50/60±1 Hz 50/60±1 Hz
Consumption: 180 VA 180 VA
Fuse: 2A 4A

Ambient Temperature and Humidity
Temperature:

15℃～35℃ (59℉～95℉)

Humidity:

10%～85% without condensation

Dimensions

Height Width Depth

46cm 39cm 40cm

Weight

25KG

Recommended Anticoagulant

A salt of K

EDTA with the proper proportion of blood to anticoagulant, as specified by

2

the tube manufacturer.

Sample Identification
An 8-digit identification number is mandatory sample identification.

Auto Hematology Analyzer Service Manual (V1.1) 1-5

General

Results Output

The system can transmit sample and control data to an external computer.
Sample results screen shows sample identification number, sample mode, sample
results and any sample result flags.
The system provides a printout of all data.

1-6 Auto Hematology Analyzer Service Manual (V1.1)

1.4 Panel Description

1.4.1 Front Panel and Keys

Figure 1-1

No. Name Description

General

1 Display Screen Display various messages, measured data and histograms
2 Keypad Touch key (all of the description refer to under lists), 23 buttons
3 Recorder Print out measured result
4 Power Light Show hematology analyzer work status
5 [Start] key Press to aspirate the sample and start counting
6 Sample Probe Aspirate the sample

Dispenses the diluent when in capillary blood mode.

Keypad

1

[START]

In Count screen, QC Count screen and Auto Calibration screen,
press it to count. In the status of Adding Diluent, press it to add
Diluent.

2

[MENU]

Press this key to switch between window operation and menu
operation

3
4

[PRINT]
[FEED]

Press this key to print using either recorder or printer
Press this key to feed paper of the recorder. Release it to stop the
operation.

5
6

[MUTE]
[DEL]

Mute the alarm and clear some of the error messages.
Delete the selected data in Review screen. Delete error message
in Error Message screen. Delete reference data and running
control data in QC Edit screen. Call default value in Normal
Range screen.

7

[0]...[9]

Auto Hematology Analyzer Service Manual (V1.1) 1-7

Enter numbers

General

8
9
10

11
12
13

14

[↑][↓][←][→]
[ID]
[DILUENT]

[PgUp][PgDn]
[ENTER]
[STARTUP]

[FLUSH]

Move the cursor in the window area or menu area.
Enter the ID number of the sample
In the Count screen of Prediluted mode, press this key to enter
the Adding Diluent status.
Scroll the screen up or down page by page.
Confirm
Clean the tubing, baths and sample probe then check the
background.
Press the key to execute the Flush operation to remove the clogs

1-8 Auto Hematology Analyzer Service Manual (V1.1)

1.4.2 Rear Panel

General

1

2

3

4

5

6

1

1

1

0

9

8

7

Figure 1-2

No. Name Description

1 Keyboard Interface Connect the standard keyboard
2 RS-232C Serial Port 1 Connect computer and transfer data to computer

CAUTION

In order to avoid any safety hazard, only coonect personal
computer which are approved to IEC950

The instrument should only be connected to an external
instrument which complies with the CISPR 11 Second
Edition 1990-09, Group 1 and Class B standard

3 RS-232C Serial Port 2 Connect a bar code scanner
4 Printer Interface Connect the external print er LX-300+ (LX-300)
5 DILUENT Tubing Connector Inlet for diluent. Connect one end of the tube (standard

accessory) to the diluent inlet and attach the other end of the
tube to the diluent

6 BNC socket for DILUENT

sensor

7 BNC socket for RINSE

sensor

Auto Hematology Analyzer Service Manual (V1.1) 1-9

connector for diluent. Connect one end of the connector of the
cable.
connector for rinse. Connect one end of the connector of the
cable.

General

8 WASTE Tubing Connector Inlet for waste. Connect one end of the tube (standard

accessory) to the waste inlet and attach the other end of the
tube to the waste

9 RINSE Tubing Connector Inlet for rinse. Connect one end of the tube (standard

accessory) to the rinse inlet and attach the other end of the
tube to the rinse

10 Equipotential ground

terminal

11 Power switch

AC source
Fuse holder

Connects the ground lead to the Equipotential ground terminal
on the wall for earth grounding
Turns power on or off
Connects the AC power cord to supply the AC power to the
instrument
Contains the time lag fuse (T 2A for 220V or T 4A for 110v)

Fuses cut the power off when an abnormality occurs in the
hematology analyzer. Remove the malfunction before
replacing the fuse.
Before replacing a fuse, turn the power off anf disconnect
the AC power cord from the instrument.
Fuse replacement should be done by a qualified person.

CAUTION

1-10 Auto Hematology Analyzer Service Manual (V1.1)

1.4.3 Front review without front panel

General

1

2

3

4

5

9

Figure 1-3
1--- Fluctuating Motor
2--- Sample Probe
3--- Sample Probe Wipe Block
4--- WBC unit shield
5--- RBC/PLT unit shield
6--- [Start] key
7---Valve 11

8--- Valve 12
9---Floppy Disk Driver

6

8

7

Auto Hematology Analyzer Service Manual (V1.1) 1-11

General

1.4.4 Right-side view without the door

2

2

3

1

2

3

4

2

2

1

2

1

0

9

1

8

1

7

1

6

1

5

1

5

6

7

8

9

1

0

1

1

4

1

3

1

2

Figure 1-4

1--- valve 8 2---volumetric unit
3---vacuum chamber 4---valve 15
5---valve 16 6---valve 14
7---valve 13 8---valve 10
9---valve 2 10--- valve 9
11---2.5ml and 50ul motor 12---10ml motor
13---2.5ml syringe 14---50ul syringe
15---10ml syringe 16---valve 4
17---valve 3 18---valve 1
19---valve 6 20---valve 5
21---valve 17 22---valve 7
23---valve 18

1-12 Auto Hematology Analyzer Service Manual (V1.1)

1.4.5 Left-side view without the door

General

Figure 1-5

1---hard disk (Module on disk)
2---vacuum pump
3---pressure pump
4---pressure chamber

Auto Hematology Analyzer Service Manual (V1.1) 1-13

General

1.5 Menu Structure Chart

Count

Whole Blood

Sample Mode

Review

Prediluted

Sample Review

Search Review

Sample Table R eview

Sample Histogram Review

Search Table Review

Search Histogram Review

Qu a lity Control

Setup

Com merical

Control

X-B Analysis

Print

Count Time

Password

Patie n t L imits

Transm ission

Date & Time

Gain

Auto Clean Time

Re ag e n t E x p. Date

Prin t C a ptio n

Parameter U n its

QC Edit

QC Count
QC Graph
QC Table

Limit

Sample /B atc h

Start/Stop

X-B Graph

X-B Table

General

Man

Woman

Ch ild

Neonate

File 1

File 1
File 2

File 3
File 4

File 5
File 6

File 7
File 8

File 2
File 3
File 4

File 5
File 6

File 7
File 8

File 9

File 9

WBC Count Time

RBC Count Time

Mid Max Width

File 1
File 2

File 3
File 4

File 5
File 6

File 7
File 8

File 9

Prin t

Select

Prin t

Format

Auto
Print

Version

Language

Type

File 1
File 2

File 3
File 4

File 5
File 6

File 7
File 8

File 9

Service

M ain te na n ce

System Status

Valve Test

Prepare to Ship

Error Messa ge

Ca libr atio n

Help

M an u a l C a lib ra ton

Au to C a lib rat ion

Shutdown

:item s ca n be v ie w e d O n ly af ter in pu t th e co rr es po n d in g pa s sword

:included items, not sub-menu

1-14 Auto Hematology Analyzer Service Manual (V1.1)

Pale tte

Recorder

Type

Chapter 2 Troubleshooting

r

A

2.1 Check Procedure

Check the instrument according to the check procedure below.

Measurement operation check
Was the quantity of each reagent sufficient fo
measurement?
Were the correct or recommended reagents used?
Was the operating temperature proper?
Was the supplied main power voltage correct?

fter turning on the instrument.
Did the instrument fail to generate an alarm?
NO DILUENT
HARDWARE FAIL

Background noise measurement.
Did the instrument fail to generate an alarm?
Was the result within the specification?

Control measurement
Did the instrument fail to generate an alarm?
Was the result reproducibility good for:
HGB? RBC?
WBC? PLT?
HCT?
(Was the obtained data within the range?)

END

before Instrument Check”

Troubleshooting

Refer to “Check Items

Refer to

“Troubleshooting

and Alarm”

Auto Hematology Analyzer Service Manual (V1.1) 2-1

Troubleshooting

2.2 Check Items before Instrument Check

Use the instrument and diluent under the following

Check items before
Instrument check

operating conditions:

Around instrument Diluent

- temperature: 15 to 35℃ temperature: 15 to 30℃

- humidity: 10 to 85%

- atmospheric pressure: 860 to 1060hPa （Working）

If the temperature is less than 15℃, it slows the
reaction rate from hemoglobin to cyanmethemoglobin.
This may result in increase of the hemoglobin data. It
may also result in increase of the WBC count because
the RBCs are not sufficiently hemolysed due to the
lower temperature. Insufficiently hemolyzed RBCs will
be included in the WBC count as RBC ghosts.

Sampled Whole Blood
Handling Check

Storage for Blood Sample

Measure all required parameters soon after sampling
the whole blood from a patient. As time elapses after
blood sampling, the blood cells’ volume and density
change. The ratios of the volume and density
variations depend on the environmental conditions and
patient. If the blood sample isleft in an air conditioned
room for a long time, the volume of the red blood cell
increases and the MCV, RDW and MPV will be
affected, and moreover, the PLT will be easily
aggregated.

WBC part differential

To get high reliability on the acquired data, measure
the blood samples within 6 hours after sampling the
whole blood. If the blood sample is left in an air
conditioned room for a long time, geerally, the WBC
membrane’s resistance against hemolysing reagent is
decreased. Therefore the WBC histogram of the
correct shape cannot be obtained.

Blood Sample from a Patient with Specified Conditions
To measure a blood from a patient who has
hepatopathy, certain special treatments, or is a
neonate, it may be necessary to use a method other
than the hematology. Analyzer. This is because the
RBC membrane’s resistance against hemolysing

2-2 Auto Hematology Analyzer Service Manual (V1.1)

Capillary Blood Handing
Check

Troubleshooting

reagent is increased (insufficient hemolysing) and it
will cause an increase of the WBC count when the
blood is measured with the hematology analyzer.

Furthermore, the bilirubin and WBC in the blood may
affect the hemoglobin concentration in the
measurement.

CAUTION

In the capillary blood mode, the instrument
aspirates the diluted sample of 20uL. In this mode,
if the venous blood is incorrectly aspirated instead
of capillary blood, there is a high possibility that
the fluid path is clogged or the background noise
is not easily decreased.

Most causes of data error using capillary blood are due
to incorrect technique for the capillary blood sampling
and diluting. Therefore, take care the following notes
and make a capillary blood sample.

NOTE

Dilute the sampled capillary blood correctly the
first time, because it is difficult to sample the
blood twice from the capillary.

Auto Hematology Analyzer Service Manual (V1.1) 2-3

Troubleshooting

2.3 How to Check Sample Data

Background Noise Check

This check is used to make sure that the counted and

calculated data of a diluent sample is not affected by
background noise. If the background checking value
exceeds the tolerable dilute data shown in the table
below, the diluent data counted and calculated before
background noise is reduced erroneous. In the table
below, each diluent data is defined as follows:

Recommended diluent data

This data is best for acquiring accurate data of the
sample.

Acceptable dilunt data

This data is the minimum value for acquiring accurate
data of the sample.

Recommended diluent Data Acceptable diluent Data

WBC 0.0 WBC
RBC 0.00 RBC
HGB 0 HGB 1g/L or 0.1g/dL
HCT 0.0 HCT 0.5%
PLT 3 PLT

Refer to “Troubleshooting Erroneous Data” of this
chapter for the possible causes of background noise
and how to reduce it.

Check Procedure

1. Press the start key to count and calculate the

2. Make sure the counted and calculated data is less

Parameter Data Check with Diluent

Check that the background values are less than or
equal to the data in the previous table. Discard the
other parameter values because they are not affected
by noise.
Especially check the data for the PLT parameters.
When the diluent includes the particles of dust smaller

9/L

10

0.3x

12/L

10

0.03x

9/L

10

10x

diluent. There is no need to aspirate the diluent
from the sampling probe.

than or equal to the acceptable diluent data as
shown upper. If they are out of range, decrease the
background noise.

2-4 Auto Hematology Analyzer Service Manual (V1.1)