Mindray BC User manual

BC-3000 Plus

Auto

Hematology

Analyzer

Service Manual

Copyright

SHENZHEN MINDRAY® BIO-MEDICAL ELECTRONICS CO., LTD. 2003

Statement

SHENZHEN MINDRAY® BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called Mindray) owns all rights to this unpublished work and intends to maintain this work as confidential. Mindray may also seek to maintain this work as an unpublished copyright. This publication is to be used solely for the purposes of reference, operation, maintenance, or repair of Mindray equipment. No part of this can be disseminated for other purposes.

In the event of inadvertent or deliberate publication, Mindray intends to enforce its rights to this work under copyright laws as a published work. Those having access to this work may not copy, use, or disclose the information in this work unless expressly authorized by Mindray to do so.

All information contained in this publication is believed to be correct. Mindray shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing, performance, or use of this material. This publication may refer to information and protected by copyrights or patents and does not convey any license under the patent rights of Mindray, nor the rights of others. Mindray does not assume any liability arising out of any infringements of patents or other rights of third parties.

Content of this manual is subject to changes without prior notice.

PROPERTY OF

SHENZHEN MINDRAY® BIO-MEDICAL ELECTRONICS CO., LTD.

ALL RIGHTS RESERVED

Responsibility on the manufacturer party

Mindray is responsible for safety, reliability and performance of this equipment only in the condition that:

Auto Hematology Analyzer Service Manual (V1.1)

i

•all installation, expansion, change, modification and repair of this equipment are conducted by Mindray qualified personnel;

•applied electrical appliance is in compliance with relevant National Standards;

•the instrument is operated under strict observance of this manual.

Note

This equipment is not intended for family usage.

This equipment must be operated by skilled/trained clinical personnel.

Warning

It is important for the hospital or organization that employs this equipment to carry out a reasonable maintenance schedule. Neglect of this may result in machine breakdown or injury of human health.

Upon request, Mindray may provide, with compensation, necessary circuit diagrams, calibration illustration list and other information to help qualified technician to maintain and repair some parts, which Mindray may define as user serviceable.

ii	Auto Hematology Analyzer Service Manual (V1.1)

Warranty

Workmanship & Materials

Mindray guarantees new equipment other than accessories to be free from defects in workmanship and materials for a period of one year from date of shipment under normal use and service. Mindray's obligation under this warranty is limited to repairing, at Mindray’s option, any part which upon Mindray's examination proves defective.

THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE.

Exemptions

Mindray's obligation or liability under this warranty does not include any transportation or other charges or liability for direct, indirect or consequential damages or delay resulting from the improper use or application of the product or the substitution upon it of parts or accessories not approved by Mindray or repaired by anyone other than a Mindray authorized representative.

This warranty shall not extend to any instrument which has been subjected to misuse, negligence or accident; any instrument from which Mindray's original serial number tag or product identification markings have been altered or removed, or any product of any other manufacturer.

Safety, Reliability and Performance

Mindray is not responsible for the effects on safety, reliability and performance of the BC-3000plus Hematology Analyzer if:

■assembly operations, extensions, re-adjusts, modifications or repairs are carried out by persons other than those authorized by Mindray.

■Personnel unauthorized by Mindray repairs or modifies the instrument.

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Return Policy

Return Procedure

In the event that it becomes necessary to return a unit to Mindray, the following procedure should be followed:

1.Obtain return authorization. Contact the Mindray Service Department and obtain a Customer Service Authorization (Mindray) number. The Mindray number must appear on the outside of the shipping container. Return shipments will not be accepted if the Mindray number is not clearly visible. Please provide the model number, serial number, and a brief description of the reason for return.

2.Freight policy. The customer is responsible for freight charges when equipment is

shipped to Mindray for service (this includes customs charges).

Company Contact

Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park, Nanshan, Shenzhen, P. R. China

Phone: [phone] Fax: [phone]

Authorized Representative

Name:	Shanghai International Holding Corp. GmbH (Europe)
Address:	Eiffestrasse 80 D-20537 Hamburg Germany
Phone:	[phone]
Fax:	[phone]

iv	Auto Hematology Analyzer Service Manual (V1.1)

Conventions Used in This Manual and Instrument

Warnings, Cautions and Notes

Warnings, cautions and notes are used in this manual to alert or signal the reader to specific information.

WARNING

Warning alerts the user to the possible injury or death associated with the use or misuse of the instrument.

CAUTION

Caution alerts the user to possible injury or problems with the instrument associated with its use or problem such as instrument malfunction, instrument failure, damage to the instrument.

NOTE

Note provides specific information, in the form of recommendations, pre-requirements, alternative goods or supplemental information.

WARNING

Potential biohazard

WARNING

Avoid contacting with the sample probe.

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Content

Content

Chapter 1 General………………………………………………...……………………………1-1

1.1	Introduction ..............................................................................................................	1-1
1.2	Service Policy...........................................................................................................	1-2
1.3	Specification.............................................................................................................	1-3
1.4	Panel Description.....................................................................................................	1-7
1.4.1 Front Panel and Keys............................................................................................		1-7
1.4.2 Rear Panel ............................................................................................................		1-9
1.4.3 Front review without front panel ..........................................................................		1-11
1.4.4 Right-side view without the door .........................................................................		1-12
1.4.5 Left-side view without the door............................................................................		1-13
1.5	Menu Structure Chart .............................................................................................	1-14
Chapter 2 Troubleshooting…………………………………………………………………….2-1
2.1	Check Procedure .....................................................................................................	2-1
2.2	Check Items before Instrument Check .....................................................................	2-2
2.3	How to Check Sample Data .....................................................................................	2-4
2.4	Troubleshooting Erroneous Data............................................................................	2-16
2.5	Troubleshooting......................................................................................................	2-21
2.6 Alarm......................................................................................................................		2-27
Chapter 3 Hardware……………………………………………………………………………3-1
3.1	CPU Board...............................................................................................................	3-2
3.2 Analog Signal Board.................................................................................................		3-6
3.3	Power Drive Board .................................................................................................	3-10
3.4	Keypad...................................................................................................................	3-13
3.5	Recorder Board......................................................................................................	3-14
3.6	Volumetric Metering Board.....................................................................................	3-16
3.7	Power Supply Board ..............................................................................................	3-18
Chapter 4 Hydraulic System…………………………………………………………………..4-1
4.1	Hydraulic System Block Diagram .............................................................................	4-1
4.2	Units of Hydraulic System ........................................................................................	4-2
4.3	Whole Blood Count Cycle ........................................................................................	4-3
4.4	Flow Charts of Main Procedures ..............................................................................	4-4
4.4.1 Power on...............................................................................................................		4-4
4.4.2 Whole Blood Count ...............................................................................................		4-5
4.4.3 Prediluted Count ...................................................................................................		4-6
4.4.4 Startup...................................................................................................................		4-7
4.4.5 Flush Apertures .....................................................................................................		4-8
4.4.6 Dispense Diluent ...................................................................................................		4-9
4.4.7 Shut Down ..........................................................................................................		4-10

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Content

4.5	Hydraulic System Flow Diagram ............................................................................	4-11
4.5.1 Inspire Sample and Diluent (Whole Blood Mode)................................................		4-11
4.5.2 Inspire Sample and Diluent (Prediluted Mode)....................................................		4-12
4.5.3 WBC Injection & Lyse Preparation ......................................................................		4-13
4.5.4 RBC & Lyse Injection ..........................................................................................		4-14
4.5.5 Mixture ................................................................................................................		4-15
4.5.6 Count Cycle.........................................................................................................		4-16
4.5.7 Cleaning..............................................................................................................		4-17
4.5.8 Flush ...................................................................................................................		4-18
4.5.9 Empty Tube.........................................................................................................		4-19
Chapter 5 System Structure…………………………………………………………………..5-1
5.1	Disassemble/Replace Parts and Components .........................................................	5-1
5.1.1 Disassemble Syringe and Replace Piston ............................................................		5-1
5.1.2 Replace Sample Probe .........................................................................................		5-7
5.1.3 Replace Sample Probe Wipe Block ....................................................................		5-12
5.1.4 Replace Vacuum Chamber .................................................................................		5-18
5.1.5 Replace Pump.....................................................................................................		5-19
5.1.6 Replace Count Bath ............................................................................................		5-22
5.1.7 Clean or Replace V11 or V12 Valve ....................................................................		5-26
5.1.8 Replace V11 or V12 ............................................................................................		5-27
5.1.9 Clean V11 or V12 ................................................................................................		5-31
5.1.10 Replace TFT Screen .........................................................................................		5-33
5.1.11 Replace Recorder Paper ...................................................................................		5-35
5.1.12 Replace Recorder Module ................................................................................		5-37
5.2	Disassemble/assemble Circuit Boards...................................................................	5-38
5.3	Connect Power Supply...........................................................................................	5-39
5.4	Connect Circuit Boards ..........................................................................................	5-41
5.4.1 CPU board connectors defined ...........................................................................		5-43
5.4.2 Power Driver Board Connectors Defined ............................................................		5-48
5.4.3 Analog Board’s connectors defined.....................................................................		5-52
5.4.4 Keyboard Connectors defined.............................................................................		5-54
5.4.5 Indicator Board Connector Defined .....................................................................		5-55
5.4.6 MTB Connector Defined......................................................................................		5-55
Chapter 6 Adjustment………………………………………………………………………….6-1
6.1	General ....................................................................................................................	6-1
6.2 Adjusted procedures ................................................................................................		6-1
6.3	Gain of WBC (whole blood and prediluted) Channel................................................	6-4
6.4	Gain of RBC Channel...............................................................................................	6-6
6.5	Gain of PLT Channel..............................................................................................	6-10
6.6	Gain of HGB Channel ............................................................................................	6-11
6.7 Adjust Display Brightness.......................................................................................		6-12
6.8 Adjust Vacuum and Pressure .................................................................................		6-13

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		Content
6.9 Adjust Count time...................................................................................................		6-14
6.10 Adjust Auto Clean Time ........................................................................................		6-16
6.11 Adjust Volumetric Metering Board ........................................................................		6-17
6.12 Re-calibrating Instrument .....................................................................................		6-19
Chapter 7 Maintenance………………………………………………………………………..7-1
7.1	Daily maintenance....................................................................................................	7-1
7.2	Monthly maintenance ...............................................................................................	7-1
7.3Half-year maintenance ..............................................................................................		7-2

Chapter 8 Spare Part List…………..………………………………………………………….8-1

Chapter 9 Performance Test…………………………………………………………………..9-1

Chapter 10 Histograms and Pulse Graphs…………………………………………………10-1

10.1	Histograms...........................................................................................................	10-1
10.2	Pulse Graphs .......................................................................................................	10-4
10.2.1 Normal Pulse Graphs........................................................................................		10-4
10.2.2 Abnormal Pulse Graphs ....................................................................................		10-6

Chapter 11 Password and Upgrade software………………………………………………11-1

11.1	Password..............................................................................................................	11-1
11.2	Upgrade System Software....................................................................................	11-2
Appendix………………………………………………………………………………………..A-1
Hardware Diagram of BC-3000PLUS.............................................................................		A-1
Hydraulic Diagram of BC-3000PLUS .............................................................................		A-2

Auto Hematology Analyzer Service Manual (V1.1)	3

General

Chapter 1 General

1.1 Introduction

CAUTION

To maintain the instrument in normal condition, the user must perform the periodic maintenance. Refer to the user manual.

This service manual provides useful information to help service personnel to understand, troubleshoot, service, maintain and repair the Hematology Analyzer.

All replaceable parts or units of this instrument and its optional units are clearly list with exploded illustration to help you locate the parts quickly.

The maintenance must be periodically performed because the instrument has fluid paths and precision parts. Accordingly, the user is responsible for performing the periodic maintenance. The “maintenance” chapter in this service manual describes the maintenance that should be performed by the qualified service personnel. The “maintenance” chapter in the user manual describes the maintenance that can be performed by the user.

NOTE

If the instrument has a problem and there has been no periodic maintenance, the instrument will usually be normal again by cleaning the fluid paths or replacing a consumable with a new one.

The information in the user manual is primarily for the user. However, it is important for service personnel to thoroughly read the user manual and service manual before starting to troubleshoot, service, maintain or repair this instrument. This is because service personnel needs to understand the operation of the instrument in order to effectively use the instrument in order to effectively use the information in the service manual.

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General

1.2 Service Policy

CAUTION

Be careful not to directly touch any place where blood is or may spread to. Wear rubber gloves to protect yourself from infection before doing maintenance.

Our company’s basic policy for technical service is to replace faulty units, printed circuit boards or parts. We do not support component-level repair of boards and units outside the factory.

NOTE

When ordering parts or accessories from your nearest distributor, please quote the part number and part name which is listed in the service manual, and the name or model of the unit in which the required part is located. This will help us to promptly attend to your needs.

Always use parts and accessories recommended or supplied by our company to assure maximum performance from your instrument.

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General

1.3 Specification

Hemoglobin Analysis

Wavelength 525nm

Sampling Features

Volumes Required for Each Analysis

Whole Blood Mode (vein blood)	13uL
Prediluted Mode (capillary blood)	20uL

Aspirated volumes

500uL of lyse first dilution per cycle for WBC measurement 300uL of second dilution per cycle for RBC and PLT measurement

Dilution Ratios	Whole Blood	Prediluted
WBC/HGB	1: 308	1:407
RBC/PLT	1:44833	1: 44274
Cell Counting Aperture Size
WBC	100um
RBC	70um
Throughput	more than 60 samples/hour

Check Diluent, Rinse and Lyse

The applied volume of each reagent is:

	Diluent	Rinse	Lyse	E-Z
Normal Startup	42ml	10ml
Prepare a sample	25.4ml	6ml	0.5ml
(whole blood)
Prepare a sample	25.1ml	6ml	0.26ml
(prediluted)
Normal Shutdown	32ml	10ml		1.6ml

Performance Specifications

Imprecision

Imprecision is based on replicate determinations of the same sample. The first

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General

result is not used in the calculation.

Imprecision Specifications

Parameter	Level	Units	CV%
WBC	7.0-15.0	109/L	≤ 2.5
RBC	3.5-6.0	1012/L	≤ 2
HGB	110 – 180	g/L	≤ 1.5
MCV	80.0 – 110.0	fL	≤ 0.5
PLT	200 – 500	109/L	≤ 5

Operating Range

	Parameter					Range		Units
	WBC				0.0-999.9			109/L
	RBC				0.00-9.99			1012/L
	HGB				0-300			g/L
	MCV				0-250			fL
	PLT				0-3000			109/L
Linearity
	Parameter		Linearity Range			Units		Difference
								(whichever is greater)
	WBC		0.3-99.9			109/L		±0.3 or ±5%
	RBC		0.20-9.99			1012/L		±0.05 or ±5%
	HGB		0-300			g/L		±2 or ±3%
	PLT		10-999			109/L		±10 or ±10%

Display

Liquid Crystal Display LCD resolution: 640×480

Input/Output

Two RS232/C serial ports

One printer port

One keyboard interface

Built-in Thermal Recorder

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General

Printer (optional)

EPSON LX-300, EPSON LX-300+

Scanner(optional)

TYSSO CCD-82
Reagents Required
DILUENT	M-30D	DILUENT
RINSE	M-30R	RINSE
LYSE	M-30L	LYSE
E-Z CLEANSER Enzyme cleanser M-30E		CLEANSER
PROBE CLEANSER	M-30P	CLEANSER
Power
Input:	AC 220V±10%	AC 110V±10%
	50/60±1 Hz	50/60±1 Hz
Consumption:	180 VA	180 VA
Fuse:	2A	4A

Ambient Temperature and Humidity

Temperature:

15 35 (59 95 )

Humidity:

10% 85% without condensation

Dimensions
Height	Width	Depth
46cm	39cm	40cm
Weight
25KG

Recommended Anticoagulant

A salt of K2EDTA with the proper proportion of blood to anticoagulant, as specified by the tube manufacturer.

Sample Identification

An 8-digit identification number is mandatory sample identification.

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General

Results Output

The system can transmit sample and control data to an external computer.

Sample results screen shows sample identification number, sample mode, sample results and any sample result flags.

The system provides a printout of all data.

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General

1.4 Panel Description

1.4.1 Front Panel and Keys

		Figure 1-1
No.	Name	Description
1	Display Screen	Display various messages, measured data and histograms
2	Keypad	Touch key (all of the description refer to under lists), 23 buttons
3	Recorder	Print out measured result
4	Power Light	Show hematology analyzer work status
5	[Start] key	Press to aspirate the sample and start counting
6	Sample Probe	Aspirate the sample
		Dispenses the diluent when in capillary blood mode.
Keypad
1	[START]	In Count screen, QC Count screen and Auto Calibration screen,
		press it to count. In the status of Adding Diluent, press it to add
		Diluent.
2	[MENU]	Press this key to switch between window operation and menu
		operation
3	[PRINT]	Press this key to print using either recorder or printer
4	[FEED]	Press this key to feed paper of the recorder. Release it to stop the
		operation.
5	[MUTE]	Mute the alarm and clear some of the error messages.
6	[DEL]	Delete the selected data in Review screen. Delete error message
		in Error Message screen. Delete reference data and running
		control data in QC Edit screen. Call default value in Normal
		Range screen.
7	[0]...[9]	Enter numbers

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General

8	[↑][↓][←][→]	Move the cursor in the window area or menu area.
9	[ID]	Enter the ID number of the sample
10	[DILUENT]	In the Count screen of Prediluted mode, press this key to enter
		the Adding Diluent status.
11	[PgUp][PgDn]	Scroll the screen up or down page by page.
12	[ENTER]	Confirm
13	[STARTUP]	Clean the tubing, baths and sample probe then check the
		background.
14	[FLUSH]	Press the key to execute the Flush operation to remove the clogs

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General

1.4.2 Rear Panel

				1
				2
				3
				4
				5
				6
11	10	9	8	7

					Figure 1-2
No.		Name			Description
1	Keyboard Interface				Connect the standard keyboard
2	RS-232C Serial Port 1				Connect computer and transfer data to computer
					CAUTION
					In order to avoid any safety hazard, only coonect personal
					computer which are approved to IEC950
					The instrument should only be connected to an external
					instrument which complies with the CISPR 11 Second
					Edition 1990-09, Group 1 and Class B standard
3	RS-232C Serial Port 2				Connect a bar code scanner
4	Printer Interface				Connect the external printer LX-300+ (LX-300)
5	DILUENT Tubing Connector				Inlet for diluent. Connect one end of the tube (standard
					accessory) to the diluent inlet and attach the other end of the
					tube to the diluent
6	BNC	socket	for	DILUENT	connector for diluent. Connect one end of the connector of the
	sensor				cable.
7	BNC	socket	for	RINSE	connector for rinse. Connect one end of the connector of the
	sensor				cable.

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General

8	WASTE Tubing Connector		Inlet for waste. Connect one end of the tube (standard
			accessory) to the waste inlet and attach the other end of the
			tube to the waste
9	RINSE Tubing Connector		Inlet for rinse. Connect one end of the tube (standard
			accessory) to the rinse inlet and attach the other end of the
			tube to the rinse
10	Equipotential	ground	Connects the ground lead to the Equipotential ground terminal
	terminal		on the wall for earth grounding
11	Power switch		Turns power on or off
	AC source		Connects the AC power cord to supply the AC power to the
	Fuse holder		instrument
			Contains the time lag fuse (T 2A for 220V or T 4A for 110v)
			CAUTION
			Fuses cut the power off when an abnormality occurs in the
			hematology analyzer. Remove the malfunction before
			replacing the fuse.
			Before replacing a fuse, turn the power off anf disconnect
			the AC power cord from the instrument.
			Fuse replacement should be done by a qualified person.

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General

1.4.3 Front review without front panel

1

2

3

4

5

6

9 8 7

Figure 1-3 1--- Fluctuating Motor 2--- Sample Probe

3--- Sample Probe Wipe Block

4--- WBC unit shield

5--- RBC/PLT unit shield

6--- [Start] key

7---Valve 11

8--- Valve 12

9---Floppy Disk Driver

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General

1.4.4 Right-side view without the door

23

22

21

20

19

1

18

2

17

3

16

4

15

5

14

6

13

7	8	9	10		11	12
		Figure 1-4
1---	valve 8			2---	volumetric unit
3---	vacuum chamber			4---	valve 15
5---	valve 16			6---	valve 14
7---	valve 13			8---	valve 10
9---	valve 2			10---	valve 9
11---	2.5ml and 50ul motor			12---	10ml motor
13---	2.5ml syringe			14---	50ul syringe
15---	10ml syringe			16---	valve 4
17---	valve 3			18---	valve 1
19---	valve 6			20---	valve 5
21---	valve 17			22---	valve 7
23---	valve 18

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General

1.4.5 Left-side view without the door

Figure 1-5

1---hard disk (Module on disk)

2---vacuum pump

3---pressure pump

4---pressure chamber

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General

1.5 Menu Structure Chart

	Count	Whole Blood
	Sample Mode
		Prediluted	Sample Table Review
	Review	Sample Review	Sample Histogram Review
		Search Review	Search Table Review
			Search HistogramReview

Quality Control

Setup

Service

Calibration

Help

Shutdown

	Commerical		QC Edit
	Control
			QC Count
			QC Graph
			QC Table
	X-B Analysis		Limit
			Sample/Batch
			Start/Stop
			X-B Graph
			X-B Table
	Print
	Count Time
	Password		General
	Patient Limits		Man
	Transmission		Woman
	Date & Time		Child
	Gain		Neonate

Auto Clean Time

Reagent Exp. Date

Print Caption

Parameter Units

Maintenance

System Status

Valve Test

Prepare to Ship

Error Message

Manual Calibraton

Auto Calibration

									File 1
						File 1
									File 2
			File 1
						File 2
									File 3
	File 1		File 2			File 3
									File 4
	File 2		File 3			File 4
									File 5
			File 4
	File 3					File 5
									File 6
	File 4		File 5			File 6
									File 7
			File 6
	File 5					File 7
									File 8
	File 6		File 7			File 8
									File 9
			File 8
	File 7					File 9
	File 8
			File 9			Print
						Select
	File 9
						Print
						Format
	WBC Count Time
						Auto
	RBC Count Time
						Print
	Mid Max Width					Version
						Language
						Type
						Palette
						Recorder
						Type

:items can be viewed Only after input the corresponding password :included items, not sub-menu

1-14	Auto Hematology Analyzer Service Manual (V1.1)

Troubleshooting

Chapter 2 Troubleshooting

2.1 Check Procedure

Check the instrument according to the check procedure below.

Measurement operation check

Was the quantity of each reagent sufficient for measurement?

Were the correct or recommended reagents used? Was the operating temperature proper?

Was the supplied main power voltage correct?

After turning on the instrument.

Did the instrument fail to generate an alarm? NO DILUENT

HARDWARE FAIL

Background noise measurement.

Did the instrument fail to generate an alarm? Was the result within the specification?

Control measurement

Did the instrument fail to generate an alarm? Was the result reproducibility good for:

HGB?	RBC?
WBC?	PLT?
HCT?

(Was the obtained data within the range?)

Refer to “Check Items before Instrument Check”

Refer to “Troubleshooting

and Alarm”

END

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Troubleshooting

2.2 Check Items before Instrument Check

Check items before Instrument check

Sampled Whole Blood

Handling Check

Use the instrument and diluent under the following

operating conditions:
Around instrument	Diluent
- temperature: 15 to 35	temperature: 15 to 30

-humidity: 10 to 85%

-atmospheric pressure: 860 to 1060hPa Working

If the temperature is less than 15 , it slows the reaction rate from hemoglobin to cyanmethemoglobin. This may result in increase of the hemoglobin data. It may also result in increase of the WBC count because the RBCs are not sufficiently hemolysed due to the lower temperature. Insufficiently hemolyzed RBCs will be included in the WBC count as RBC ghosts.

Storage for Blood Sample

Measure all required parameters soon after sampling the whole blood from a patient. As time elapses after blood sampling, the blood cells’ volume and density change. The ratios of the volume and density variations depend on the environmental conditions and patient. If the blood sample isleft in an air conditioned room for a long time, the volume of the red blood cell increases and the MCV, RDW and MPV will be affected, and moreover, the PLT will be easily aggregated.

WBC part differential

To get high reliability on the acquired data, measure the blood samples within 6 hours after sampling the whole blood. If the blood sample is left in an air conditioned room for a long time, geerally, the WBC membrane’s resistance against hemolysing reagent is decreased. Therefore the WBC histogram of the correct shape cannot be obtained.

Blood Sample from a Patient with Specified Conditions To measure a blood from a patient who has hepatopathy, certain special treatments, or is a neonate, it may be necessary to use a method other than the hematology. Analyzer. This is because the RBC membrane’s resistance against hemolysing

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Troubleshooting

reagent is increased (insufficient hemolysing) and it will cause an increase of the WBC count when the blood is measured with the hematology analyzer.

Furthermore, the bilirubin and WBC in the blood may affect the hemoglobin concentration in the measurement.

Capillary Blood Handing

Check

CAUTION

In the capillary blood mode, the instrument aspirates the diluted sample of 20uL. In this mode, if the venous blood is incorrectly aspirated instead of capillary blood, there is a high possibility that the fluid path is clogged or the background noise is not easily decreased.

Most causes of data error using capillary blood are due to incorrect technique for the capillary blood sampling and diluting. Therefore, take care the following notes and make a capillary blood sample.

NOTE

Dilute the sampled capillary blood correctly the first time, because it is difficult to sample the blood twice from the capillary.

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Troubleshooting

2.3 How to Check Sample Data

Background Noise Check This check is used to make sure that the counted and calculated data of a diluent sample is not affected by background noise. If the background checking value exceeds the tolerable dilute data shown in the table below, the diluent data counted and calculated before background noise is reduced erroneous. In the table below, each diluent data is defined as follows:

Recommended diluent data

This data is best for acquiring accurate data of the sample.

Acceptable dilunt data

This data is the minimum value for acquiring accurate data of the sample.

Recommended diluent Data		Acceptable diluent Data
WBC	0.0	WBC	0.3x109/L
RBC	0.00	RBC	0.03x1012/L
HGB	0	HGB	1g/L or 0.1g/dL
HCT	0.0	HCT	0.5%
PLT	3	PLT	10x109/L

Refer to “Troubleshooting Erroneous Data” of this chapter for the possible causes of background noise and how to reduce it.

Check Procedure

1.Press the start key to count and calculate the diluent. There is no need to aspirate the diluent from the sampling probe.

2.Make sure the counted and calculated data is less than or equal to the acceptable diluent data as shown upper. If they are out of range, decrease the background noise.

Parameter Data Check with Diluent

Check that the background values are less than or equal to the data in the previous table. Discard the other parameter values because they are not affected by noise.

Especially check the data for the PLT parameters. When the diluent includes the particles of dust smaller

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