Mindray BC-2800 Operator's Manual

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BC-2800

Auto Hematology Analyzer

Operator’s Manual

For this Operator’s Manual, the issued date is 2009-06.

Intellectual Property Statement

SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called

Mindray) owns the intellectual property rights to this Mindray product and this manual. This

manual may refer to information protected by copyright or patents and does not convey any

license under the patent rights or copyright of Mindray, or of others.

Mindray intends to maintain the contents of this manual as confidential information.

Disclosure of the information in this manual in any manner whatsoever without the written

permission of Mindray is strictly forbidden.

Release, amendment, reproduction, distribution, rental, adaptation, translation or any other

derivative work of this manual in any manner whatsoever without the written permission of

Mindray is strictly forbidden.

, are the trademarks, registered or otherwise, of Mindray in China and

other countries. All other trademarks that appear in this manual are used only for

informational or editorial purposes. They are the property of their respective owners.

Responsibility on the Manufacturer Party

Contents of this manual are subject to change without prior notice.

All information contained in this manual is believed to be correct. Mindray shall not be liable

for errors contained herein or for incidental or consequential damages in connection with the

furnishing, performance, or use of this manual.

Mindray is responsible for the effects on safety, reliability and performance of this product,

only if:

 all installation operations, expansions, changes, modifications and repairs of this product

are conducted by Mindray authorized personnel;

 the electrical installation of the relevant room complies with the applicable national and

local requirements; and

 the product is used in accordance with the instructions for use.

z It is important for the hospital or organization that employs this equipment

to carry out a reasonable service/maintenance plan. Neglect of this may result in machine breakdown or personal injury.

z Be sure to operate the analyzer under the situation specified in this manual;

otherwise , the analyze r will not w ork normall y and the ana lysis resul ts will be unreliable, which would damage the analyzer components and cause personal injury.

z This equipment must be operated by skilled/trained clinical professionals.

Warranty

THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,

EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR

FITNESS FOR ANY PARTICULAR PURPOSE.

Definitions

 Main unit: Integrated facilities which implement the specified function separately.

Generally speaking, the main unit should include power supply, control system and

some functional modules.

 Accessories: Materials connected to the main unit to extend or implement specified

function.

 Consumables: Disposable or short-life parts which should be replaced each time after

use or periodically

Exemptions

Mindray's obligation or liability under this warranty does not include any transportation or

other charges or liability for direct, indirect or consequential damages or delay resulting from

the improper use or application of the product or the use of parts or accessories not approved

by Mindray or repairs by people other than Mindray authorized personnel.

This warranty shall not extend to:

 Malfunction or damage caused by improper use or man-made failure.  Malfunction or damage caused by unstable or out-of-range power input.  Malfunction or damage caused by force majeure such as fire and earthquake.  Malfunction or damage caused by improper operation or repair by unqualified or

unauthorized service people.

 Malfunction of the instrument or part whose serial number is not legible enough.  Others not caused by instrument or part itself.

The standard warranty period is as below:

 Main unit: 18 months from shipment  Accessories: 15 months from shipment  Consumables: N/A

III

Return Policy

Return Procedure

In the event that it becomes necessary to return this product or part of this product to Mindray,

the following procedure should be followed:

1. Return authorization: Contact the international Customer Service Department and obtain

a Return Materials Authorization number. This number must appear on the outside of the

shipping container. Returned shipments will not be accepted if the number is not clearly

visible. Please provide the model number, serial number, and a brief description of the

reason for return.

2. Freight policy: The customer is responsible for freight charges when this product is

shipped to Mindray for service (this includes customs charges).

3. Return address: Please send the part(s) or equipment to the address offered by the

international Customer Service Department.

Company Contact

Manufacturer: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

E-mail Address:

Fax:

EC-Representative: Shanghai International Holding Corp. GmbH(Europe)

Address:

Tel:

service@mindray.com.cn

+86 755 26582479 26582888

Tel:

+86 755 26582934 26582500

Eiffestraβe 80, Hamburg 20537, Germany

0049-40-2513175

Fax:

0049-40-255726

Table of Contents

1 Using This Manual................................................................................... 1-1

1.1 Introduction ............................................................................................ 1-1

1.2 Who Should Read This Manual ............................................................. 1-2

1.3 How to Find Information......................................................................... 1-3

1.4 Conventions Used in This Manual ......................................................... 1-4

1.5 Special Terms Used in This Manual....................................................... 1-5

1.6 Symbols ................................................................................................. 1-6

2 Understanding Your Analyzer.................................................................2-1

2.1 Introduction ............................................................................................ 2-1

2.2 Intended Use.......................................................................................... 2-2

2.3 User Interface......................................................................................... 2-3

2.3.1 LCD ............................................................................................. 2-6

2.3.2 Input Devices .............................................................................. 2-6

2.3.3 Recorder...................................................................................... 2-8

2.3.4 Keyboard Interface...................................................................... 2-8

2.3.5 Serial Ports.................................................................................. 2-8

2.3.6 Parallel Port................................................................................. 2-8

2.3.7 Power Supply for the Floppy Disk Drive ..................................... 2-8

2.3.8 Power Indicator ........................................................................... 2-8

2.3.9 Optional Devices ......................................................................... 2-8

2.4 Instrument Software............................................................................... 2-9

2.4.1 Main Screen................................................................................ 2-9

2.4.2 Screen saver............................................................................. 2-10

2.4.3 System Menu ............................................................................ 2-11

2.5 Reagents, Controls and Calibrator....................................................... 2-13

2.5.1 Diluent....................................................................................... 2-13

2.5.2 Lyse........................................................................................... 2-13

2.5.3 Rinse......................................................................................... 2-14

2.5.4 E-Z Cleanser ............................................................................. 2-14

2.5.5 Probe Cleanser ......................................................................... 2-14

2.5.6 Controls and Calibrator ............................................................. 2-14

3 Understanding the System Principles................................................... 3-1

3.1 Introduction ............................................................................................ 3-1

3.2 Aspiration ............................................................................................... 3-2

3.3 Dilution ................................................................................................... 3-3

3.4 WBC/HGB Measurement....................................................................... 3-4

3.4.1 Volumetric Metering .................................................................... 3-4

3.4.2 Measurement Principles.............................................................. 3-5

Table of Contents

3.4.3 Derivation of WBC-Related Parameters ..................................... 3-6

3.4.4 HGB............................................................................................. 3-7

3.5 RBC/PLT Measurement ......................................................................... 3-8

3.5.1 Volumetric Metering .................................................................... 3-8

3.5.2 Measurement Principles.............................................................. 3-9

3.5.3 Derivation of RBC-Related Parameters...................................... 3-9

3.5.4 Derivation of PLT-Related Parameters ..................................... 3-11

3.6 Wash .................................................................................................... 3-12

4 Installing Your Analyzer .......................................................................... 4-1

4.1 Introduction ............................................................................................ 4-1

4.2 Installation Requirements....................................................................... 4-2

4.2.1 Space Requirements ................................................................... 4-2

4.2.2 Power Requirements................................................................... 4-2

4.2.3 General Environment .................................................................. 4-3

4.3 Unpacking .............................................................................................. 4-4

4.3.1 Unpacking and Inspecting the Analyzer...................................... 4-4

4.3.2 How to move the analyzer........................................................... 4-4

4.4 Installation Procedure ............................................................................ 4-5

4.4.1 Connecting Regents.................................................................... 4-5

4.4.2 Installing Recorder Paper............................................................ 4-9

4.4.3 Connecting the Keyboard.......................................................... 4-10

4.4.4 Connecting the Printer (Optional) ............................................. 4-10

4.4.5 Connecting the Bar-Code Scanner (Optional) .......................... 4-10

4.5 Starting the Analyzer ............................................................................ 4-11

5 Customizing the Analyzer Software....................................................... 5-1

5.1 Introduction ............................................................................................ 5-1

5.2 Password ............................................................................................... 5-2

5.2.1 Entering the Administrator Password .......................................... 5-2

5.2.2 Resuming the Common User Password..................................... 5-3

5.3 Editing Settings ...................................................................................... 5-4

5.3.1 Reagent....................................................................................... 5-5

5.3.2 Printing and Communication Settings ......................................... 5-6

5.3.3 Date and Time........................................................................... 5-11

5.3.4 Gain........................................................................................... 5-13

5.3.5 Count......................................................................................... 5-16

5.3.6 Reference Range ...................................................................... 5-20

5.3.7 Auto Maintain ............................................................................ 5-23

5.3.8 Other Settings ........................................................................... 5-25

6 Operating Y our Analyzer.........................................................................6-1

6.1 Introduction ............................................................................................ 6-1

6.2 Initial Checks.......................................................................................... 6-2

Table of Contents

6.3 Power-on................................................................................................ 6-3

6.4 Daily Quality Control .............................................................................. 6-4

6.5 Selecting Analysis Mode ........................................................................ 6-5

6.6 Sample Collection and Handling............................................................ 6-6

6.6.1 Whole Blood Samples................................................................. 6-6

6.6.2 Prediluted Samples..................................................................... 6-7

6.7 Running Whole Blood Samples ............................................................. 6-9

6.7.1 Entering Sample Information ...................................................... 6-9

6.7.2 Running the Samples................................................................ 6-15

6.7.3 Special Functions...................................................................... 6-16

6.8 Running Prediluted Samples ............................................................... 6-19

6.8.1 Entering Sample Information .................................................... 6-19

6.8.2 Running the Samples................................................................ 6-24

6.8.3 Special Functions...................................................................... 6-25

6.9 Shutdown ............................................................................................. 6-28

7 Reviewing Sample Results..................................................................... 7-1

7.1 Introduction ............................................................................................ 7-1

7.2 Browsing All Sample Results ................................................................. 7-2

7.2.1 Browsing in the Histogram Mode................................................ 7-2

7.2.2 Browsing in the “Table” mode...................................................... 7-7

7.3 Searching for Interested Sample Results ............................................ 7-17

7.3.1 Starting a Search....................................................................... 7-17

7.3.2 Reviewing search result in the “Table” mode ............................ 7-19

7.3.3 Reviewing Search Result in the “Histogram” Mode.................. 7-29

8 Using the QC Programs.......................................................................... 8-1

8.1 Introduction ............................................................................................ 8-1

8.2 QC With Controls ................................................................................... 8-2

8.2.1 QC Editing................................................................................... 8-2

8.2.2 Running the Controls .................................................................. 8-6

8.2.3 Reviewing QC Results .............................................................. 8-10

8.3 X-B Analysis ......................................................................................... 8-15

8.3.1 QC Editing................................................................................. 8-15

8.3.2 Running X-B Analysis................................................................ 8-18

8.3.3 Reviewing X-B Results.............................................................. 8-18

9 Using the Calibration Programs.............................................................9-1

9.1 Introduction ............................................................................................ 9-1

9.2 When to Calibrate .................................................................................. 9-2

9.3 How to Calibrate..................................................................................... 9-3

9.3.1 Preparing Your Analyzer.............................................................. 9-3

9.3.2 Calibration Using Calibrator Program ......................................... 9-4

9.3.3 Fresh Blood Calibration............................................................. 9-10

Table of Contents

9.3.4 Manual Calibration Program ..................................................... 9-17

10 Maintaining Your Analyzer....................................................................10-1

10.1 Introduction .......................................................................................... 10-1

10.2 General Guidelines .............................................................................. 10-2

10.3 Using the “Maintenance” Program ....................................................... 10-3

10.3.1 Diluent Prime............................................................................. 10-4

10.3.2 Rinse Prime............................................................................... 10-5

10.3.3 Lyse Prime ................................................................................ 10-6

10.3.4 Zap Aperture ............................................................................. 10-7

10.3.5 Flush Aperture........................................................................... 10-8

10.3.6 Probe Cleanser Cleaning.......................................................... 10-9

10.3.7 E-Z Cleanser Cleaning............................................................ 10-11

10.3.8 Lyse Test ................................................................................. 10-13

10.3.9 Clean Bath .............................................................................. 10-15

10.3.10 Drain Bath............................................................................. 10-16

10.3.11 Drain Tubing ......................................................................... 10-18

10.3.12 Clean Probe Wipe................................................................. 10-19

10.3.13 Prepare to Ship..................................................................... 10-21

10.4 Using the “Status” Program................................................................ 10-23

10.5 Using the “Self-test” Program ............................................................ 10-24

10.5.1 Testing the Fluidic System ...................................................... 10-25

10.5.2 Testing Motors and Recorder/Printer ...................................... 10-25

10.5.3 Testing Valves ......................................................................... 10-26

10.5.4 Testing A/D Interrupt................................................................ 10-27

10.6 Log ..................................................................................................... 10-28

10.7 Viewing System Configuration ........................................................... 10-30

10.8 Printing Management......................................................................... 10-31

10.9 Calibrating Sample Probe Position .................................................... 10-32

10.10 Replacing the Probe Wipe ................................................................. 10-36

10.11 Replacing the Filter of the Vacuum Chamber.................................... 10-37

10.12 Maintaining Recorder ......................................................................... 10-39

11 Troubleshooting Your Analyzer............................................................ 11-1

11.1 Introduction .......................................................................................... 11-1

11.2 Errors without available error messages ............................................. 11-2

11.3 Errors indicated by error messages..................................................... 11-3

12 Appendices .............................................................................................. A-1

A Index.........................................................................................................A-1

B Specifications ..........................................................................................B-1

Table of Contents

C Precautions, Limitations and Hazards...................................................C-1

D Communication (8ID communication protocol)....................................D-1

E Communication (15ID communication protocol)..................................E-1

1 Using This Manual

1.1 Introduction

This chapter explains how to use your operator’s manual, which is shipped with your

BC-2800 auto hematology analyzer and contains reference information about the BC-2800

and procedures for operating, troubleshooting and maintaining the analyzer. Read this

manual carefully before operating your analyzer and operate your analyzer strictly as

instructed in this manual.

z Operate your analyzer strictly as instructed in this manual. z All illustrations in this manual are provided as examples only. They may not

necessarily reflect your analyzer setup or data displayed and must not be used for any other purpose.

1-1

Using This Manual

1.2 Who Should Read This Manual

This manual contains information written for clinical laboratory professionals to

 learn about the BC-2800 hardware and software;

 customize system settings;

 perform daily operating tasks;

 perform system maintenance and troubleshooting.

1-2

Using This Manual

1.3 How to Find Information

This operator’s manual comprises 11 chapters and 4 appendices. Refer to the table below to

find the information you need.

If you want to … See …

learn about the intended use and parameters of the BC-2800

learn about the hardware and software of the BC-2800

learn about how the BC-2800 works

learn about how to install the BC-2800

learn about how to define/adjust system settings

learn about how to use the BC-2800 to perform your daily

operating tasks

learn about how to review the saved analysis results

learn about how to use the quality control programs

Chapter 2 Understanding Y our Analyzer Chapter 2 Understanding Y our Analyzer Chapter 3 Understanding the System Principles Chapter 4 Installing Your Analyzer Chapter 5 Customizing the Analyzer Software Chapter 6 Operating Your Analyzer Chapter 7 Reviewing Sample Results Chapter 8 Using the QC

learn about how to calibrate the BC-2800

learn about how to maintain/service the BC-2800

learn about the meanings of the error messages and how to

correct the problems

learn about the technical specifications of the BC-2800

see the summary of all safety messages included in this

manual

learn about the communication protocol of the BC-2800

1-3

Programs Chapter 9 Using the Calibration Programs Chapter 10 Maintaining Your Analyzer Chapter 11 Troubleshooting Y our Analyzer Appendix B Specifications Appendix C Precautions, Limitations and Hazards Appendix D Communication

Using This Manual

1.4 Conventions Used in This Manual

This manual uses certain typographical conventions to clarify meaning in the text:

 All capital letters enclosed in [ ] indicate a key name (either on the built-in keypad or the

external keyboard), such as [ENTER].

 All capital, bold and italic letters indicate a special operation defined in the following

section, such as SELECT.

 Bold letters included in “ ” indicate text you can find on the screen, such as “Prepare to

ship”.

 Bold letters indicate defined screen areas/fields, such as System Status area, or

chapter titles, such as Chapter 1 Using This Manual.

All illustrations in this manual are provided as examples only. They may not necessarily

reflect your analyzer setup or data displayed.

1-4

Using This Manual

1.5 Special Terms Used in This Manual

When you read … It means …

CLICK

ENTER

DELETE

to press the arrow keys ([←][→] [↑][↓]) as needed to move the

cursor to a certain software button on screen and press

[ENTER].

to press the arrow keys ([←][→] [↑][↓]) as needed to move

cursor to the desired edit box and use the built-in keypad or

the external keyboard to enter the desired characters or digits.

Note that besides the numeric keys you may also use the

[PgUp] or [PgDn] keys to enter digits; or to scan the number

using the bar-code scanner.

to press the arrow keys ([←][→] [↑][↓]) as needed to move the

cursor to the character or digit to the left of the one you want to

delete and press [DEL]; or to press the arrow keys

([←][→][↑][↓]) as needed to move the cursor to the character

or digit to the right of the one you want to delete and press

[BackSpace] on the external keyboard.

to move the cursor to the character or digit you want to change

MODIFY

SELECT from “ ** ”

pull-down list

SELECT

and re-enter the desired one using either the built-in keypad or

the external keyboard.

to press the arrow keys ([←][→] [↑][↓]) as needed to move the

cursor to the desired edit box and press [ENTER] to display

the pull-down list and press [↑] or [↓] to move the cursor to the

desired item and press [ENTER] to select it.

to press the arrow keys ([←][→] [↑][↓]) as needed to the

desired item and press [ENTER].

z This analyzer adopts a fixed decimal point. You can enter the digits without

bothering to look for the [.] on the external keyboard.

1-5

Using This Manual

1.6 Symbols

You will find the following symbols in this manual.

When you see… Then…

read the statement below the symbol. The statement is

You may find the following symbols on the analyzer or the reagents.

When you see… It means…

alerting you to an operating hazard that can cause

personnel injury.

read the statement below the symbol. The statement is

alerting you to a possibility of analyzer damage or unreliable

analysis results.

read the statement below the symbol. The statement is

alerting you to information that requires your attention.

read the statement below the symbol. The statement is

alerting you to a potentially biohazardous condition.

EQUIPOTENTIALITY

CAUTION, CONSULT ACCOMPANYING

DOCUMENTS.

BIOLOGICAL RISK

HIGH VOLTAGE

ALTERNATING CURRENT

USE BY

1-6

Using This Manual

SERIAL NUMBER

IN VITRO DIAGNOSTIC

DATE OF MANUFACTURE

TEMPERATURE LIMITATION

CONSULT INSTRUCTIONS FOR USE

THE DEVICE IS FULLY CONFORMANCE

WITH THE COUNCIL DIRECTIVE

CONCERNING IN VITRO DIAGNOSTIC

MEDICAL DEVICES 98/79/EC.

MANUFACTURER

AUTHORISED REPRESENTATIVE IN THE

EUROPEAN COMMUNITY

IRRITATING SUBSTANCE

THE FOLLOWING DEFINITION OF THE

WEEE LABEL APPLIES TO EU MEMBER

STATES ONLY: THE USE OF THIS SYMBOL

INDICATES THAT THIS PRODUCT SHOULD

NOT BE TREATED AS HOUSEHOLD

WASTE. BY ENSURING THAT THIS

PRODUCT IS DISPOSED OF CORRECTLY,

YOU WILL HELP PREVENT BRINGING

POTENTIAL NEGATIVE CONSEQUENCES

TO THE ENVIRONMENT AND HUMAN

HEALTH. FOR MORE DETAILED

INFORMATION WITH REGARD TO

1-7

Using This Manual

RETURNING AND RECYCLING THIS

PRODUCT, PLEASE CONSULT THE

DISTRIBUTOR FROM WHOM YOU

PURCHASED THE PRODUCT.

1-8

Using This Manual

（4）

（3）

（2）

（1）

Figure 1-1 Back of the analyzer

(1)

Equipotentiality.

（2）

 Connect only to a properly earth grounded outlet;

 To avoid electric shock, disconnect power cord prior to removing or replacing fuse;

 Replace fuse only with the type and rating specified.

（3）

Biological risk.

（4）

The following definition of the WEEE label applies to EU member states only: The use of this

symbol indicates that this product should not be treated as household waste. By ensuring that

1-9

Using This Manual

this product is disposed of correctly, you will help prevent bringing potential negative

consequences to the environment and human health. For more detailed information with

regard to returning and recycling this product, please consult the distributor from whom you

purchased the product.

（5）

Figure 1-2 Warning label

（5）

To avoid being injured, do not put hand under the motor when the machine is running.

1-10

Using This Manual

（6）

Figure 1-3 Warning label

（6）

To avoid electrical shock, disconnect the power supply before maintaining this device.

1-11

Using This Manual

（7）

High Voltage

Figure 1-4 High voltage warning label

1-12

2 Understanding Your Analyzer

2.1 Introduction

The BC-2800 Auto Hematology Analyzer is a quantitative, automated hematology analyzer

and leukocyte differential counter for In Vitro Diagnostic Use in clinical laboratories.

2-1

Understanding Your Analyzer

2.2 Intended Use

z The purpose of this analyzer is to identify the normal patient, with all normal

system-generated parameters, and to flag or identify patient results that require additional studies.

The analyzer is used for the quantitative determination of a maximum of 19 parameters and 3

histograms of blood samples.

White Blood Cell or leukocyte WBC

Lymphocyte Lymph#

Mid-sized cell Mid#

Granulocyte Gran#

Lymphocyte percentage Lymph%

Mid-sized cell percentage Mid%

Granulocyte percentage Gran%

Red Blood Cell or erythrocyte RBC

Hemoglobin Concentration HGB

Mean Corpuscular (erythrocyte) Volume MCV

Mean Cell (erythrocyte) Hemoglobin MCH

Mean Cell (erythrocyte) Hemoglobin Concentration

MCHC

Red Blood Cell (erythrocyte) Distribution Width

Coefficient of Variation

Red Blood Cell (erythrocyte) Distribution Width

Standard Deviation

Hematocrit HCT

Platelet PLT

Mean Platelet Volume MPV

Platelet Distribution Width PDW

Plateletcrit PCT

White Blood Cell Histogram WBC Histogram

Red Blood Cell Histogram RBC Histogram

Platelet Histogram PLT Histogram

2-2

RDW-CV

RDW-SD

2.3 User Interface

Understanding Your Analyzer

Figure 2-1 Front view

1 ---- LCD 2 ---- Keypad

3 ---- Recorder 4 ---- Power indicator

5 ---- Aspirate key 6 ---- Sample probe

2-3

Understanding Your Analyzer

Figure 2-2 Back view

1 --- Power switch 2 --- Equipotentiality

3 --- Waste outlet(Red) 4 --- Lyse inlet(Orange)

5 --- Rinse inlet(Blue) 6 --- Diluent inlet(Green)

2-4

Understanding Your Analyzer

Figure 2-3 Left view

1 --- RS-232 port1 2 --- Parallel port

3 --- RS-232 port2 4 --- Power Interface of Floppy Disk Drive

5 --- Keyboard interface

2-5

Understanding Your Analyzer

2.3.1 LCD

The LCD is located on the front panel of the analyzer, as Figure 2-4 shows. It displays all

alphanumeric and graphic data.

2.3.2 Input Devices

The input devices include the aspirate key, built-in keypad and PS/2 keyboard.

 Aspirate key

The aspirate key is located behind the sample probe, as Figure 2-4 shows. You can press the

key to start the selected run cycle or dispense diluent.

Figure 2-4 Sample probe and aspirate key

1.Sample probe 2. Aspirate key

2-6

Understanding Your Analyzer

 Built-in keypad

The 18-key keypad is located below the LCD, as Figure 2-5 shows.

Figure 2-5 Build-in keypad

 PS/2 keyboard

The analyzer can also be controlled by an external PS/2 keyboard that should be connected

to the analyzer’s keyboard interface. See Table 2-1 for the correspondence between the

keypad keys and the keyboard keys and for their functions.

Table 2-1 Key functions

Keypad PS/2 keyboard Function

[MENU] [Esc] Press it to enter/exit the system menu.

[PRINT] [P] or [p] Press it to print out data by the recorder or printer.

[HELP] [H] or [h] Press it to call out the analyzer’s on-line help.

[DEL] [Delete] or [Del] Press it to delete data and characters.

[ENTER] [Enter] Press it to confirm or execute an operation

[↑], [↓],

[←], [→]

[F1], [F2], [F3],

[F4], [F5]

[PgUp][PgDn] [PageUp]

[↑], [↓],

[←], [→]

[F1], [F2], [F3],

[F4], [F5]

Press them to move the cursor.

Press them to use various functions. See the rest of this

manual or the analyzer’s on-line help for details.

Press them to scroll the screen.

[PageDown]

[MODE] [Ctrl+A] Switch to another analysis mode (works only in the

Count screen).

[DILUENT] In the prediluted mode, press it if you want to dispense

diluent into the sample tube.

/ Other keys Use them as needed. See the rest of this manual or the

analyzer’s on-line help for details.

2-7

Understanding Your Analyzer

2.3.3 Recorder

A thermal recorder is located on the front panel. It prints out analysis reports and other related

information.

2.3.4 Keyboard Interface

A PS/2 keyboard can be connected here.

2.3.5 Serial Ports

The analyzer provides two RS-232 ports, one for connecting the scanner and the other for

connecting a computer (host).

2.3.6 Parallel Port

The analyzer provides a parallel port to connect a printer or a floppy disk drive (a floppy disk

drive is needed to upgrade the system software; the drive can only be connected by a

Mindray-supplied cable).

2.3.7 Power Supply for the Floppy Disk Drive

It supplies power to the connected floppy disk drive. Only the drive power cable supplied by

Mindraycan be used.

2.3.8 Power Indicator

The power indicator tells you whether the analyzer is on, off or in the screen saver mode.

2.3.9 Optional Devices

 Printer

An external printer can be connected to the parallel port at the left side of the analyzer. You

can use it to print out a detailed report and other desired information.

 Bar-code scanner

A bar-code scanner can be connected to the RS-232 port 1 of the analyzer. You can use it to

scan the bar-coded sample IDs and reagent information into the analyzer.

z Use the printer and scanner of the specified model.

2-8

Understanding Your Analyzer

2.4 Instrument Software

2.4.1 Main Screen

After finishing the startup procedure, the analyzer enters the “Count” screen, which is the

screen to be used most frequently, hence the name is main screen.

Title Area Count Mode Area System Status Area

Error Message

Area

 Title Area The Title area displays the title of the current screen, which, in case of Figure 2-6, is “Count”.

 Count Mode Area The Count Mode area displays in which analysis (count) mode, the next sample is to be

analyzed. In case of Figure 2-6, the next sample is to be analyzed in the “Whole Blood-All”.

 System Status Area The System Status area displays whether this analyzer is ready for the next analysis. When

it displays “Ready”, it means this analyzer is ready and you can proceed to analyze the next sample. When it displays “Waiting”, it means the analyzer is not ready for the next run yet. When it displays “Running”, it means this analyzer is analyzing a sample.

Figure 2-6 “Count” screen

Help Area

Reagent Status

Area

 System Time Area

2-9

Understanding Your Analyzer

The System Time area displays the system time.

 Sample Information Area The Sample Information area has two sub-areas, the upper titled “Current sample” and the

lower “Next sample”.

The “Current sample” refers to the sample, whose analysis result is displayed on the “Count” screen. Its sample ID, time of analysis, analysis mode and patient information (name, gender, age), are respectively displayed in the fields of the “Current sample” area.

The “Next Sample” refers to the sample to be analyzed next. Its sample ID and analysis mode are displayed in the “Next sample” area.

 Analysis Result Area The Analysis Result area displays the analysis result, including histograms, of the current

sample.

 Error Message Area The Error Message area displays error messages one by one, alternating every two

seconds.

 Reagent Status Area The Reagents Status area displays how many counts the remaining reagents are enough for.

Note that when it displays “99 counts”, it indicates the reagents are enough for over 99

counts and there is also enough space left in the waste container for the counts; when it

displays “0 counts”, it indicates either at least one of the reagents is insufficient or the waste

container is full.

 Menu Area

When you press [MENU], this area displays the system menu.

 Help Area The Help area reminds you how to proceed to the next step.

2.4.2 Screen saver

This analyzer will enter the screen saver if it has been idle at the “Count” screen for 10

minutes. When it happens, the sample probe will retract into the analyzer, the LCD will turn

dark and the power indicator will be flickering. You can press any key to resume the display

and reposition the sample probe.

2-10

Understanding Your Analyzer

2.4.3 System Menu

Press the [MENU] button and the system menu, shown in Figure 2-7 below, will pop up.

Figure 2-7 System menu

The system menu contains 7 programs. The programs followed by “>”s have further

sub-menus. See Figure 2-8 for the expanded menu.

Figure 2-8 Fully expanded system menu

2-11

Understanding Your Analyzer

You can select the desired program as instructed below.

If you want to… Select…

analyze samples

review sample results

run the QC program

customize system software

maintain/service the analyzer

calibrate the analyzer

shut down the analyzer

Count Review Quality Control Setup Service Calibration Shutdown

2-12

Understanding Your Analyzer

2.5 Reagents, Controls and Calibrator

Because the analyzer, reagents (diluent, rinse, lyse, probe cleanser and E-Z cleanser),

controls, and calibrator are components of a system, performance of the system depends on

the combined integrity of all components. You should only use the Mindray-specified reagents

(see Appendix B Specifications), which are formulated specifically for the fluidic system of

your analyzer in order to provide optimal system performance. If other reagents are used, the

analyzer may not meet the performance specified in this manual and may provide unreliable

results. All references related to reagents in this manual refer to the reagents specifically

formulated for this analyzer.

Each reagent package must be examined before use. Inspect the package for signs of

leakage or moisture. Product integrity may be compromised in packages that have been

damaged. If there is evidence of leakage or improper handling, do not use the reagent.

z Store and use the reagents as directed by instructions for use of the

reagents.

z When you have changed the diluent, rinse or lyse, run a background to see

if the results meet the requirement.

z Pay attention to the expiration dates and open-container stability days of all

the reagents. Never use expired reagents.

z After installing new reagents, let the reagents stand for a while before using

them.

2.5.1 Diluent

The diluent is formulated to meet the following requirements:

 To dilute the blood samples;

 To provide the blood cells with an environment similar to the blood plasma;

 To maintain the cell volume of each red blood cell and platelet during the count and

sizing portion of the measurement cycle;

 To provide a conductive medium for impedance counting of white and red blood cells

and platelets.

2.5.2 Lyse

The lyse is formulated to meet the following requirements:

2-13

Understanding Your Analyzer

 To rapidly break down red blood cell walls, release the hemoglobin from the cell, and

reduce the size of cellular debris to a level that does not interfere with white blood cell

counting.

 To convert hemoglobin to a complex whose absorbance is determined by the

hemoglobin concentration.

2.5.3 Rinse

The rinse is formulated to rinse the bath and metering tubes and to provide proper meniscus

formation in the metering tubes and maintain it during each measurement cycle.

2.5.4 E-Z Cleanser

The E-Z (enzymatic) cleaner is an enzyme-based isotonic, cleaning solution and wetting

agent formulated to clean the fluidic lines and bath.

2.5.5 Probe Cleanser

The probe cleanser is an alkaline cleaning solution formulated to clean the fluidic lines,

apertures and bath.

2.5.6 Controls and Calibrator

The controls and calibrator are used to verify accurate operation of and calibrate the

analyzer.

The controls are commercially prepared whole-blood products used to verify that the analyzer

is functioning properly. They are available in low, normal, and high levels. Daily use of all

levels verifies the operation of the analyzer and ensures reliable results are obtained. The

calibrators are commercially prepared whole-blood products used to calibrate the analyzer.

Read and follow the instructions for use to use the controls and calibrators. All references

related to controls and calibrators in this manual refer to the controls and calibrators reagents

specifically formulated for this analyzer. Controls and calibrators can be purchased from

Mindray or Mindray-authorized distributors.

2-14

3 Understanding the System

Principles

3.1 Introduction

The two independent measurement methods used in this analyzer are:

 the impedance method for determining the WBC, RBC, and PLT data;

 the colorimetric method for determining the HGB.

During each analysis cycle, the sample is aspirated, diluted and mixed before the

determination for each parameter is performed.

3-1

Understanding the System Principles

3.2 Aspiration

This analyzer can process two types of blood samples – whole blood samples and prediluted

blood samples.

If you are going to analyze a whole blood sample, you can simply present the sample to the

sample probe and press the aspirate key to aspirate 13µL of the sample into the analyzer.

If you are going to analyze a capillary blood sample, you should first manually dilute the

sample (20 µL of capillary sample needs to be diluted by 1.6 mL of diluent) and then present

the pre-diluted sample to the sample probe and press the aspirate key to aspirate 0.7 mL of

the sample into the analyzer.

3-2

Understanding the System Principles

3.3 Dilution

Usually in blood samples, the cells are too close to each other to be identified or counted. For

this reason, the diluent is used to separate the cells so that they are drawn through the

aperture one at a time as well as to create a conductive environment for cell counting.

Moreover, red blood cells usually outnumber white blood cells by 1,000 times. For this reason,

lyse needs to be added to the sample to eliminate the red cells before the WBC counting.

3-3

Understanding the System Principles

3.4 WBC/HGB Measurement

3.4.1 Volumetric Metering

An accurate cell count cannot be obtained unless the precise volume of diluted sample that

passes through the aperture during the count portion of the analysis cycle (the count cycle) is

known. This analyzer uses a volumetric metering unit to control the count cycle and to ensure

that a precise volume of sample is analyzed.

The metering unit controlling the WBC count cycle consists of a metering tube with two

optical sensors mounted on it, as Figure 3-1 shows. This tube ensures that a precise amount

of diluted sample is measured during each count cycle. The exact amount is determined by

the distance between the two optical sensors. The rinse is used to create a meniscus in the

metering tube. The count cycle starts when the meniscus reaches the upper sensor and

stops when the meniscus reaches the lower sensor. The amount of time required for the

meniscus to travel from the upper sensor to the lower sensor is called the WBC Count Time

and is measured in seconds. At the end of the count cycle, the measured count time is

compared to the pre-defined reference count time (see Chapter 5.3 for details). If the former

is less than or greater than the latter by 2 seconds or more, the analyzer will report WBC

bubbles or WBC clog error. Seeing the error message, you can refer to Chapter 11

Troubleshooting Your Analyzer for solutions.

Figure 3-1 Volumetric metering process

3-4

Understanding the System Principles

3.4.2 Measurement Principles

 WBC measurement

WBCs are counted and sized by the impedance method, as Figure 3-2 shows. This method is

based on the measurement of changes in electrical resistance produced by a particle, which

in this case is a blood cell, suspended in a conductive diluent as it passes through an

aperture of known dimensions. An electrode is submerged in the liquid on both sides of the

aperture to create an electrical pathway. As each particle passes through the aperture, a

transitory change in the resistance between the electrodes is produced. This change

produces a measurable electrical pulse. The number of pulses generated indicates the

number of particles that passed through the aperture. The amplitude of each pulse is

proportional to the volume of each particle. Each pulse is amplified and compared to the

internal reference voltage channels, which only accepts the pulses of certain amplitude. If the

pulse generated is above the WBC threshold, it is counted as a WBC.

Figure 3-2 Impedance method of counting and sizing

 HGB measurement

HGB is determined by the colorimetric method. The WBC/HGB dilution is delivered to the

bath where it is bubble mixed with a certain amount of lyse, which converts hemoglobin to a

hemoglobin complex that is measurable at 525 nm. An LED is mounted on one side of the

bath and emits a beam of monochromatic light, whose central wavelength is 525nm, and then

is measured by a photo-sensor that is mounted on the opposite side. The signal is then

amplified and the voltage is measured and compared to the blank reference reading

(readings taken when there is only diluent in the bath). The HGB is calculated per the

following equation and expressed in g/L.

3-5

Understanding the System Principles

HGB(g/L) = Constant×Log

(Blank Photocurrent/Sample Photocurrent)

3.4.3 Derivation of WBC-Related Parameters

 WBC

WBC (10

passing through the aperture.

Note that NRBCs do not react with the lyse and can be mistaken by the analyzer for white

blood cells. If you observe NRBCs in the microscope, correct the system-generated result by

the following formula,

where WBC represents the system-generated white cell number, NRBC the number of

NRBCs counted in 100 white cells and WBC′ the corrected white cell number.

/ L) is the number of leukocytes measured directly by counting the white blood cells

WBC'WBC

＝ ×

×=

100

＋

L/10nWBC

NRBC100

 WBC differentia

With the help of the diluent and lyse, this analyzer can size the white cells into three

sub-populations - lymphocytes, mid-sized cells (including monocytes, basophils and

eosinophils) and granulocytes. Based on the WBC histogram, this analyzer calculates Lymph ％, Mid％ and Gran％ as follows and express the results in percents.

100

PGPMPL

100

PGPMPL

100

PGPMPL

L/10

)

L/10

)

L/10

where PL = particles in the lymphocyte region(

PM = particles in the mid size region(

PG = particles in the granulocyte region(

Lymph% ×

Mid% ×

Gran% ×

Having achieved the three parameters above, this analyzer proceeds to calculate the

Lymph# , Mid# and Gran# per the following equations and express them in

3-6

L/10

Understanding the System Principles

 WBC histogram

Besides the parameters mentioned above, this analyzer also presents a WBC histogram, whose x-coordinate represents the cell volume（fL）and y-coordinate represents the number

of the cells. The histogram is presented in the Analysis Result area of the “Count” screen

when the analysis is done. You can also review the histograms of the stored patient results

(see Chapter 7 Reviewing Sample Results).

The first three discriminators of the WBC histogram can be adjusted in case you are not

satisfied with the result. Note that you cannot adjust them if the WBC result is less than 0.5 or

out of the operating range.

#Lymph

#Mid

100

#Gran

100

WBC%Lymph

100

WBC%Mid

WBC%Gran

3.4.4 HGB

Using the colorimetric method, this analyzer calculates hemoglobin concentration (g/L) as

follows.

HGB (g/L)=Constant×Log

(Blank Photocurrent/Sample Photocurrent)

3-7

Understanding the System Principles

3.5 RBC/PLT Measurement

3.5.1 Volumetric Metering

An accurate cell count cannot be obtained unless the precise volume of diluted sample that

passes through the aperture during the count cycle is known. This analyzer uses a volumetric

metering unit to control the count cycle and to ensure that a precise volume of sample is

analyzed for the measurement.

The metering unit controlling the RBC/PLT count cycle consists of a metering tube with two

optical sensors mounted on it, as Figure 3-3 shows. This tube ensures that a precise amount

of diluted sample is measured during each count cycle. The exact amount is determined by

the distance between the two optical sensors. The rinse is used to create a meniscus in the

metering tube. The count cycle starts when the meniscus reaches the upper sensor and

stops when the meniscus reaches the lower sensor. The amount of time required for the

meniscus to travel from the upper sensor to the lower sensor is called the RBC Count Time

and is measured in seconds. At the end of the count cycle, the measured count time is

compared to the pre-defined reference count time (see Chapter 5.3 for details). If the former

is less than or greater than the latter by 2 seconds or more, the analyzer will report RBC

bubbles or RBC clog error. Seeing the error message, refer to Chapter 11 Troubleshooting

Your Analyzer for solutions.

Figure 3-3 Volumetric metering process

3-8

Understanding the System Principles

3.5.2 Measurement Principles

 RBC/PLT measurement

RBCs/PLTs are counted and sized by the impedance method, as Figure 3-4 shows. This

method is based on the measurement of changes in electrical resistance produced by a

particle, which in this case is a blood cell, suspended in a conductive diluent as it passes

through an aperture of known dimensions. An electrode is submerged in the liquid on both

sides of the aperture to create an electrical pathway. As each particle passes through the

aperture, a transitory change in the resistance between the electrodes is produced. This

change produces a measurable electrical pulse. The number of pulses generated indicates

the number of particles that passed through the aperture. The amplitude of each pulse is

proportional to the volume of each particle. Each pulse is amplified and compared to the

internal reference voltage channels, which only accepts the pulses of a certain amplitude. If

the pulse generated is above the RBC/PLT lower threshold, it is counted as an RBC/PLT.

Figure 3-4 Impedance method of counting and sizing

3.5.3 Derivation of RBC-Related Parameters

 RBC

RBC (10 passing through the aperture.

 MCV

Based on the RBC histogram, this analyzer calculates the mean cell volume (MCV) and

/L) is the number of erythrocytes measured directly by counting the erythrocytes

3-9

Understanding the System Principles

expresses the result in fL.

This analyzer calculates the HCT (%), MCH (pg) and MCHC (g/L) as follows:

Where the RBC is expressed in 10

 RDW-CV

Based on the RBC histogram, this analyzer calculates the CV (Coefficient of Variation) of the

erythrocyte distribution width.

HCT

MCH =

MCHC ×=

MCVRBC

HGB RBC

HGB

100

HCT

/L, MCV in fL and HGB in g/L.

 RDW-SD

RDW-SD (RBC Distribution Width – Standard Deviation, fL) is set on the 20% frequency level

with the peak taken as 100%, as Figure 3-5 shows.

Figure 3-5 RBC Distribution Width – Standard Deviation

 RBC Histogram

Besides the parameters mentioned above, this analyzer also presents an RBC histogram, whose x-coordinate represents the cell volume（fL）and y-coordinate represents the number

of the cells. The histogram is presented in the Analysis Result area of the “Count” screen

when the analysis is done. You can also review the histograms of the stored patient results

(see Chapter 7 Reviewing Sample Results).

The two discriminators of the RBC histogram can be adjusted in case you are not satisfied

with the result. Note that you cannot adjust them if the RBC result is less than 0.2 or out of the

operating range.

3-10

Understanding the System Principles

3.5.4 Derivation of PLT-Related Parameters

 PLT

PLT (10

 MPV

Based on the PLT histogram, this analyzer calculates the mean platelet volume (MPV, fL).

 PDW

Platelet distribution width (PDW) is the geometric standard deviation (GSD) of the platelet

size distribution. Each PDW result is derived from the platelet histogram data and is reported

as 10 (GSD).

 PCT

/L) is measured directly by counting the platelets passing through the aperture.

This analyzer calculates the PCT as follows and express it in ％.

Where the PLT is expressed in 10

PCT

/L and the MPV in fL.

MPVPLT

10000

 PLT Histogram

Besides the parameters mentioned above, this analyzer also presents a PLT histogram, whose x-coordinate represents the cell volume（fL）and y-coordinate represents the number

of the cells. The histogram is presented in the Analysis Result area of the “Count” screen

when the analysis is done. You can also review the histograms of the stored patient results

(see Chapter 7 Reviewing Sample Results).

The two discriminators of the PLT histogram can be adjusted in case you are not satisfied

with the result. Note that you cannot adjust them if the PLT result is less than 10 or out of the

operating range.

3-11

Understanding the System Principles

3.6 Wash

After each analysis cycle, each element of the analyzer is washed.

 The sample probe is washed internally and externally with diluent;

 The bath is washed with diluent and rinse;

 The metering tube is washed with rinse;

 The rest of the fluidic system is washed by diluent.

3-12

4 Installing Your Analyzer

4.1 Introduction

This chapter introduces how to install the BC-2800. To ensure all system components

function correctly and to verify system performance, Mindray-authorized representatives will

handle the installation and initial software setup.

z Installation by personnel not authorized or trained by Mindray may damage

your analyzer. Do not install your analyzer without the presence of Mindray-authorized personnel.

4-1

Installing Your Analyzer

4.2 Installation Requirements

Before installation, you should ensure that the following space, power and environmental

requirements are met.

4.2.1 Space Requirements

Check the site for proper space allocation. In addition to the space required for the analyzer

itself, arrange for

 at least 28 cm on each side, which is the preferred access to perform service

procedures;

 at least 10 cm behind for cabling and ventilation;

 enough room on or below the countertop to accommodate the diluent, rinse and waste

containers.

4.2.2 Power Requirements

Check the availability of a power outlet that meets the following requirements

 Voltage: 100 to 240 VAC;

 Frequency: 50/60±1 Hz

 Power: 180VA

 Fuse: 250V T4A

z Make sure the analyzer is properly grounded. z Replace fuse only with the type and rating specified. z Make sure the electrical outlet meets the requirements.

z Before connecting the power cord, make sure the power switch at the back

of the analyzer is placed in the off (O) position.

4-2

Installing Your Analyzer

4.2.3 General Environment

 Operating temperature: 15 ℃ to 30 ℃.

 Relative humidity: 30% to 85%.

 Atmospheric pressure: 60 kPa to 106 kPa.

 The environment should be as free as possible from dust, mechanical vibrations, loud

noises, and electrical interference.

 Do not place the analyzer near brush-type motors, flickering fluorescent lights, and

electrical contacts that regularly open and close.

 Do not place the analyzer in direct sunlight or in front of a source of heat or drafts.

z Do not place the analyzer in a flammable or explosive environment.

z Do not place any container on the top of the analy zer.

z If the ambient temperature is outside the specified operating range, the

analyzer will alarm you for abnormal ambient temperature and the analysis results may be unreliable. See Chapter 11 Troubleshooting Your Analyzer for solutions.

4-3

Installing Your Analyzer

4.3 Unpacking

4.3.1 Unpacking and Inspecting the Analyzer

Your analyzer is tested before it is shipped from the factory. International symbols and special

handling instructions tell the carrier how to treat this electronic instrument. When you receive

your analyzer, carefully inspect the carton. If you see any signs of mishandling or damage,

contact Mindray customer service department or your local distributor immediately. When you

are sure the carton is fine, follow the steps below to unpack the analyzer:

 Place the carton on the floor upright with the arrows on the side upwards;

 Remove the tape and take out the accessory box. Check the accessories against the

packing list. Notify the Mindray customer service department or your local distributor

immediately if you find anything missing;

 Open the main box and check the items inside against the packing list. Notify the

Mindray customer service department or your local distributor immediately if you find

anything missing;

 Remove the top protective foam, carefully carry out the analyzer from the box and place

it on the countertop.

 Remove the foam fixing the sample probe.

z Retain the shipping carton and all the packing materials, as they can be

used for packaging if analyzer must be reshipped.

4.3.2 How to move the analyzer

 If the carton is intact, you may use a plate and fork-lifter to move the analyzer for a short

distance.

 If your analyzer has been used, do the ”Empty tubing” procedure and shut it down

before moving it.

 For a short - distance moving on a smooth ground, you may use a trolley to facilitate the

transportation.

 During the moving process, protect the LCD and the sample probe from excessive force

and from contact with other objects.

 Keep the analyzer upright during the moving process. Do not tilt or incline it.

 Do your best to minimize the mechanical shock when moving the analyzer. After a

long-distance moving, check and tune the analyzer before using it.

4-4

Installing Your Analyzer

4.4 Installation Procedure

z Dispose of reagents, waste, samples, consumables, etc. according to

government regulations.

z The reagents are irritating to eyes, skin and diaphragm. Wear proper

personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.

z If the reagents accidentally spill on your skin, wash them off with plenty of

water and if necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and immediately go see a doctor.

z Use the specified reagents. z After installing new reagents, keep the reagents still for a while before using

them.

z Never use expired reagents. z T o prevent c ont amination, tighte n the cont ainer c ap s when the inst allation is

finished.

z Samples, controls, calibrator and waste are potentially infectious. Wear

proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.

4.4.1 Connecting Regents

On the back of the instrument you will find a fluidic connection equipped with 4 plastic plugs.

These plugs are closed by protection caps to prevent dust and the leaking of liquids during

transportation. Take off these caps by unscrewing them and keep them in a safe place for

future transportation.

Connecting the diluent container

1. Take out the diluent pickup tube (the one with a green connector) from the accessory box

4-5

Installing Your Analyzer

（Figure 4-1）;

Figure 4-1 Diluent container

2. Take out the diluent container and place it on or below the countertop;

3. Remove the container cap and insert the tube end that has no connector into the diluent

container and tighten the cap until properly secured, as Figure 4-2 shows;

Figure 4-2 Insert the tube end into the container

4. Locate the green fitting marked “DILUENT” in the lower right corner of the back of the

analyzer;

5. Plug the green connector of the tube into the fitting and turn it clockwise until properly

secured.

Connecting the rinse container

1. Take out the rinse pickup tube (the one with a blue connector) from the accessory box, as

Figure 4-3 shows;

4-6

Installing Your Analyzer

Figure 4-3 Rinse container

2. Take out the rinse container and place it on or below the countertop;

3. Remove the container cap and insert the tube end that has no connector into the rinse

container and tighten the cap until properly secured, as Figure 4-4 shows;

Figure 4-4 Insert the tube end into the container

4. Locate the blue fitting marked “RINSE” in the lower right corner of the back of the

analyzer.

5. Plug the blue connector of the tube into the fitting and turn it clockwise until properly

secured.

Connecting the lyse container

1. Take out the lyse pickup tube (the one with an orange connector) from the accessory box,

as Figure 4-5 shows;

4-7

Installing Your Analyzer

Figure 4-5 Lyse container

2. Take out the lyse container;

3. Remove the container cap and insert the tube end that has no connector into the

container and tighten the cap until properly secured, as Figure 4-6 shows;

Figure 4-6 Insert the tube into the container

4. Locate the orange fitting marked “LYSE” in the lower right corner of the back of the

analyzer;

5. Plug the orange connector of the tube into the fitting and turn it clockwise until properly

secured.

Connect the waste container

1. Take out the waste tube (the one with a red connector) from the accessory box;

2. Locate the red fitting marked “WASTE” in the lower right corner of the back of the

analyzer;

3. Plug the red connector of the tube into the fitting and turn it clockwise until properly

secured;

4. Prepare a container to receive the waste and place it on or below the countertop;

5. Insert the waste tube into the waste container.

4-8

Installing Your Analyzer

4.4.2 Installing Recorder Paper

z Remove the protective paper between the recorder head and the roller

inside the recorder before installing recorder paper.

Follow the procedure below to install the recorder paper.

1. Use the latch at the upper right corner of the recorder door to pull the door open.

2. Insert a new roll into the compartment as shown below.

3. Close the recorder door.

4. Check if paper is installed correctly and the paper end is feeding from the top.

Paper roll

Figure 4-7 Installing recorder paper

z Use only specified recorder paper. Otherwise, it may cause damage to the

recorder head, or the recorder may be unable to print, or poor print quality may result.

z Never pull the recorder paper with force when a recording is in process.

Otherwise, it may cause damage to the recorder.

z Do not leave the recorder door open unless you install paper or remove

trouble.

z Improper installation of recorder paper may jam the paper and/or result in

blank printout.

4-9

Installing Your Analyzer

4.4.3 Connecting the Keyboard

Take out the keyboard from the accessory kit and connect it to the keyboard interface marked

“KB”.

4.4.4 Connecting the Printer (Optional)

Follow the printer’s instructions for use to connect the printer to the parallel port.

4.4.5 Connecting the Bar-Code Scanner (Optional)

Follow the scanner’s instructions for use to connect the scanner to the serial port1.

z Use the printer and scanner of the specified model.

4-10

Installing Your Analyzer

4.5 Starting the Analyzer

Take out the power cord from the accessory box. Plug the non-pronged end into the AC input

at the back of the analyzer and the pronged end into an electrical outlet. Place the power

switch at the back of the analyzer in the ON position (1) to turn on the analyzer. The power

indicator light will be illuminated and the screen will display “Initializing…“. The analyzer will

sequentially initialize the file, hardware and fluidic systems and the whole initializing process

lasts about 4 to 7 minutes. When the initialization is finished, the analyzer will automatically

enter the “Count” screen.

4-11

5 Customizing the Analyzer Software

5.1 Introduction

The BC-2800 is a flexible laboratory instrument that can be tailored to your work environment.

You can use the “Setup” program to customize the software options as introduced in Chapters 5.2 to 5.3.

5-1

Customizing the Analyzer Software

5.2 Password

The BC-2800 classifies users into two categories: common users (default) and administrators.

You need to enter the administrator password to adjust certain options such as “Count”, “Gain”, etc.

5.2.1 Entering the Administrator Password

Press [MENU] to enter the system menu.

Figure 5-1 System menu

SELECT “Setup → Password” ( Figure 5-1 ) to enter the ”Password” screen ( Figure 5-2 ).

Figure 5-2 “Password” screen

ENTER “2826” and press [MENU], a message box will pop up to remind you of the current

password level, as Figure 5-3 shows, to remind you of the current password level,

5-2

Customizing the Analyzer Software

Figure 5-3 A message box to confirm the user level

CLICK “Yes” to confirm the password and exit to the system menu.

5.2.2 Resuming the Common User Password

Enter the “Password” screen and the default password is the common user password. Press

[MENU] again and a message box will pop up to remind you of the current password level, as

Figure 5-4 shows.

Figure 5-4 A message box to confirm the user level

CLICK “Yes” to confirm the password and exit to the system menu.

5-3

Customizing the Analyzer Software

5.3 Editing Settings

You can use the “Settings” menu to edit system settings.

Press [MENU] to enter the system menu. SELECT “Setup → Settings“, as Figure 5-5 shows, to enter the “Settings” screen, as Figure 5-6 shows.

Figure 5-5 System menu

Figure 5-6 “Settings” screen

This screen can be interpreted as follows:

 Setting Groups area (on the left)

This area displays the visible or changeable setting groups. You can press [F1] to select the desired group. The selected group is preceded by a ⊙.

 Settings area (on the right)

You can change the settings of the items displayed in this area.

 Help area (on the bottom)

5-4

Customizing the Analyzer Software

This area displays useful information to help you move to the next step.

At this screen, if you want to acquire help information, press [HELP]; if you want to return to

the system menu, press [MENU].

5.3.1 Reagent

You can select the “Reagent” group to change the settings regarding the reagents and the

waste, as Figure 5-7 shows.

Figure 5-7 “Reagent” settings

 Selecting the “Reagent” group Press [F1] to select the “Reagent” group.

 Setting remaining volumes for reagents

You may set the remaining volumes for the diluent, rinse and lyse. When any of the entered

volumes is counted down to zero, the system will remind you to install a new container.

1. SELECT “Diluent”, “Rinse” or “Lyse” in the “Remains” field as needed;

2. ENTER the desired digits. See Table 5-1 for the valid reagent volumes.

Table 5-1 Valid reagent volumes

Allowed range

 Entering the usable volume of the waste container

About 0 to 30.0 L About 0 to 30.0 L About 0 to 999.0 mL

Diluent Rinse Lyse

You may enter the usable volume of the waste container. When the system counts down the

entered volume to 0, it will alert you to empty the waste container. Follow the steps given

below to set the volume.

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Customizing the Analyzer Software

1. SELECT “Waste Cont ainer”;

2. ENTER the desired digits.

 Entering expiration dates of reagents

You can specify the expiration dates for the diluent, rinse and lyse. Once any of these

reagents is expired, the system will alert you to install a new container. Follow the steps given

below to enter the expiration dates.

1. SELECT “Exp. Date”;

2. ENTER the desired digits. You can use the bar-code scanner (if available) to scan the

bar-code of the reagents into the analyzer;

3. Note that open reagents are stable for 60 days. The entered expiration date should be

the open date + 60 days or the expiration date marked on the packaging of the reagent,

whichever is earlier.

 Exiting the “Reagent” group

When you have finished changing all the desired reagent settings, you may

1. Press [F1] to select another setting group you want to change; or

2. Press [MENU] and a message box will pop up to remind you to save the changes, as

Figure 5-8 shows. CLICK “Yes” to save the changes and exit to the system menu; or

CLICK “No” to exit to the system menu without saving the changes.

Figure 5-8 Saving changes

Note that if any entered value is beyond the valid range, a message box will pop up after you

have pressed [MENU]. CLICK “Yes” to close the message box and clear the invalid values.

Figure 5-9 A message box to remind you of the erroneous entry

5.3.2 Printing and Communication Settings

You can select the “Print & comm.” group to change the printing and communication settings

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Customizing the Analyzer Software

to your own need, as Figure 5-10 shows.

Figure 5-10 Printing and communication settings

 Selecting the “Print & comm.” group Press [F1] to select the “Print & comm.” group.

 Selecting the printing device

To select a printing device, SELECT “Recorder” or “Printer” from the “Device” drop-down list, as Figure 5-10 shows.

 Selecting the printing format

If you have selected the printer, you can choose either of the following printing formats.

1. Format1 - One page with histogram;

2. Format2 - One page without histogram.

To select a printing format, SELECT desired format from the “Format” drop-down list,

If you have selected the recorder, you can choose any of the following 4 printing formats.

1. Format1 - parameter values + histograms;

2. Format2 – parameter values only;

3. Format3 - parameter values + histograms;

4. Format4 - parameter values only.

To choose the desired format, SELECT the desired format from the “Format” pull-down list,

as Figure 5-11 shows.

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 Auto printing

Customizing the Analyzer Software

Figure 5-11 Selecting printing format

The auto printing function refers to the analyzer’s ability to automatically print out the analysis

results once they are done. To activate this function, SELECT “ON” (or “OFF”) from the “Auto” pull-down list, as Figure 5-12 shows.

Figure 5-12 Selecting auto-printing

 Setting baud rate

To choose one of the four baud rates, “19200”, “9600”, “4800”, “2400” and “1200”, SELECT the desired baud rate from the “Baud” drop-down list, as Figure 5-13 shows.

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Customizing the Analyzer Software

Figure 5-13 Selecting baud rate

 Selecting parity

To choose the “Odd”, “Even” or “None” (default) check, SELECT the desired check from the “Parity” drop-down list, as Figure 5-14 shows.

Figure 5-14 Setting parity

 Activating/deactivating handshake

If the “Handshake” function is activated, to start the transmission this analyzer will send a

handshake signal to an external computer and wait for the response. If the computer does not

respond, this analyzer will abort the transmission and give an alarm for the transmission error.

If the “Handshake” function is deactivated, this analyzer will transmit data to the external

computer regardless of the response. This function is deactivated by default.

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Customizing the Analyzer Software

To activate or deactivate this option, SELECT “Yes” or ”No” from the “Handshake” drop-down list, as Figure 5-15 shows.

Figure 5-15 Setting handshake

 Auto Communication

The auto communication function refers to the analyzer’s ability to automatically transmit the

analysis results to an external computer once they are done. To activate or deactivate this

function, SELECT “On” or ”Off” from the “Auto” drop-down list in the “Transmit” field, as

Figure 5-16 shows.

Figure 5-16 Auto communication

 Editing report titles (external keyboard needed)

To edit the title of an analysis report,

1. SELECT “Recorder” or ”Printer” in the “Report Title” field, depending on the selected

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Customizing the Analyzer Software

printing device;

2. ENTER the desired report title.

 Exiting the “Print & comm.” group

When you have finished changing all the desired printing and transmission settings, you may

press [F1] to select another setting group you want to change; or press [MENU] and a

message box will pop up to remind you to save the changes, as Figure 5-17 shows. CLICK “Yes” to save the changes and exit to the system menu; or CLICK “No” to exit to the system

menu without saving the changes.

Figure 5-17 Saving changes

5.3.3 Date and Time

You can select the “Date & time” group to set the system date and time, as Figure 5-18

shows. Follow the instructions given below to do so.

Figure 5-18 Setting date and time

 Selecting the “Date & time” group Press [F1] to select the “Date & time” group.

 Setting the system date

1. SELECT “Year”, ” Month” or ”Day”;

2. ENTER the desired digits.

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Customizing the Analyzer Software

 Selecting the date format

You may choose one of the three formats “YYYY-MM-DD”, “MM-DD-YYYY“ and “DD-MM-YYYY”. To do so, SELECT the desired format from the “Format” drop-down list,

as Figure 5-19 shows.

Figure 5-19 Setting date format

 Setting the system time

1. SELECT “Hour”, “Minute” or “Second”;

2. ENTER the desired digits.

 Exiting the “Date & time” group

When you have finished changing all the desired date and time settings, you may

1. Press [F1] to select another setting group you want to change; or;

2. Press [MENU] and a message box will pop up to remind you to save the changes, as

Figure 5-20 shows. CLICK “Yes” to save the changes and exit to the system menu; or

CLICK “No” to exit to the system menu without saving the changes.

Figure 5-20 Saving changes

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Customizing the Analyzer Software

5.3.4 Gain

You can select the “Gain” group to view or change (if you have the administrator password)

the WBC, RBC and HGB gains.

Figure 5-21 Setting gain

 Selecting the “Gain” group Press [F1] to select the “Gain” group, as Figure 5-21 shows.

 Setting the WBC gain

When WBC histograms of most samples are similar to Figure 5-22, it implies too small a

WBC gain and you need to increase the gain appropriately.

Figure 5-22 WBC gain too small

When WBC histograms of most samples are similar to Figure 5-23, it implies too large a WBC

gain and you need to decrease the gain appropriately.

Figure 5-23 WBC gain too large

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Customizing the Analyzer Software

To increase (or decrease) the gain

1. Enter the administrator password as introduced in Chapter 5.2.1.

2. At the ”Gain” screen and ENTER the desired gain into the “WBC (WB) ”, as Figure 5-24 shows, or “WBC (PB)”, as Figure 5-25 shows.

Figure 5-24 Setting WBC (WB) gain

Figure 5-25 Setting WBC (PB) gain

 Setting the RBC gain

When the difference between the actual MCV result and the expected result exceeds 6%,

you need to change the RBC gain.

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Customizing the Analyzer Software

For example, assuming the expected MCV result is 90.0fL, while the actual analysis result is

82.0fL, then

VExpectedMC

%100 =×× ＝

ActualMCV

You should adjust the RBC gain to 109.8% as close as possible. Follow the steps given below

to do so.

1. Enter the administrator password as introduced in Chapter 5.2.1;

2. At the “Settings” screen, press [F1] to select the “Gain” group;

3. SELECT “RBC”, as Figure 5-26 shows;

4. ENTER the desired gain so that the adjustment becomes as close to 109.8% as possible.

0.90 %8.109%100

0.82

Figure 5-26 Setting RBC gain

 Setting the HGB gain

You may adjust the HGB gain to change the HGB blank voltage, which usually should be set

between 3.4 to 4.8V (4.5V recommended). Follow the steps given below to set the HGB gain.

1. Enter the administrator password as introduced in Chapter 5.2.1;

2. At the “Settings” screen, press [F1] to select the “Gain” group;

3. SELECT “HGB”;

4. ENTER the desired gain so that the HGB blank voltage is between 3.4 to 4.8V, as Figure

5-27 shows.

5-15

 Exiting “Gain” group

Customizing the Analyzer Software

Figure 5-27 Setting HGB gain

When you have finished changing all the desired gain settings, you may

1. Press [F1] to select another setting group you want to change; or;

2. Press [MENU] and a message box will pop up to remind you to save the changes, as

Figure 5-28 shows. CLICK “Yes” to save the changes and exit to the system menu; or

CLICK “No” to exit to the system menu without saving the changes.

Figure 5-28 Saving changes

5.3.5 Count

You can select the “Count” group to view or change (if you have the administrator password)

the parameter units and count time.

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Customizing the Analyzer Software

Figure 5-29 Setting unit and count time

 Selecting the “Count” group Press [F1] to select the “Count” group, as Figure 5-29 shows.

 Selecting parameter units

This analyzer provides multiple units for certain parameters. Refer to Table 5-2 for all the

selectable units of all parameters. The 19 parameters are divided into 11 groups based on

their units and you can only select unit for the first parameter of a group. Pay special attention

to the HGB group, which includes HGB, MCHC and MCH. When you select g/L or g/dL as the

unit of HGB, the default unit for MCH is pg; when you select mmol/L as the unit of HGB, the

default unit of MCH is fmol.

Table 5-2 Units of Parameters

Parameter Display format Unit Remarks

WBC

Lymph#

Mid#

Gran#

***.* 109/L Default

***.* 103/uL /

**** 102/uL /

***.* /nL /

Mid%

Gran%

HGB, MCHC

**.* Lymph%

.***

Default

*** g/L Default

**.* g/dL /

**.* mmol/L /

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Customizing the Analyzer Software

RBC

*.** 1012/L Default

*.** 106/uL /

*** 104/uL /

*.** /pL /

**.* % Default HCT

.*** L/L /

***.* fL Default MCV, RDW-SD

***.* um

RDW-CV **.* % Default

PLT

**** 109 /L Default

**** 103 /uL /

***.* 104 /uL /

**** /nL /

***.* fL Default MPV

***.* um3 /

PDW **.* / Default

.*** % Default PCT

*.** mL/L /

MCH

*.*** pg Default

**.** fmol /

Follow the steps given below to select the units.

1. Enter the administrator password as introduced in Chapter 5.2.1;

2. At the “Settings” screen, press [F1] to select the “Count” group;

3. SELECT the desired unit from the drop-down list of the desired parameter, as Figure

5-30 shows.

5-18

 Setting count time

Customizing the Analyzer Software

Figure 5-30 Selecting parameter unit

If the WBC or RBC count time is inappropriately set, the system may give false alarms for

clogs or bubbles. When this happens, follow the steps given below to change the WBC or

RBC count time. Refer to the actual count time (see Chapter 10.5.1 for details) when editing

the count time. Follow the steps given below to set the count time.

1. Enter the administrator password as introduced in Chapter 5.2.1;

2. At the “Settings” screen, press [F1] to select the “Count” group;

3. SELECT “WBC” or “RBC”, as Figure 5-31 shows;

4. ENTER the desired digits.

 Exiting the “Count” group

Figure 5-31 Setting count time

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Customizing the Analyzer Software

When you have finished changing all the parameter units and count time settings you want to

change, you may

1. Press [F1] to select another setting group you want to change; or;

2. Press [MENU] and a message box will pop up to remind you to save the changes, as

Figure 5-32 shows. CLICK “Yes” to save the changes and exit to the system menu; or

CLICK “No” to exit to the system menu without saving the changes.

Figure 5-32 Saving changes

5.3.6 Reference Range

You can set a reference range for every parameter. The system will flag any analysis result

that exceeds this range with either an H or L. This analyzer divides the patients into 5 patient groups, which are all listed in Table 5-3. The default ranges are given. Note that the default

ranges are for references only. Each laboratory is encouraged to establish its own

references.

Table 5-3 Demographic groups

Group Gender Age

Not specified, male or female. Not specified. General

Not specified.

Man Male

Woman Female

＞ 12 years

Child Male or Female

Neonate Male or Female

The upper and lower limits of the reference ranges are visible to all users but changeable

5-20

＞ 28 days and ≤12 years

≤ 28 days

Customizing the Analyzer Software

only to administrators, as Figure 5-33 shows. Follow the instructions below to set the ranges.

Figure 5-33 Reference range screen

 Selecting patient group

Follow the steps given below to select the patient group you want.

1. At the “Settings” screen, press [F1] to select the “Ref. Range” group.

2. SELECT the combo box to the right of “Group”.

Figure 5-34 Selecting patient group

3. SELECT “General”, “Man”, “Woman”, “Child” or “Neonate” from the “Group”

pull-down list.

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Customizing the Analyzer Software

 Setting reference ranges (administrators only)

1. Enter the administrator password as introduced in Chapter 5.2.1;

2. At the “Settings” screen, press [F1] to select the “Ref. Range” group, as Figure 5-35

shows;

3. When you have finished selecting the patient group, SELECT the desired parameter and ENTER the desired digits for the upper and lower limits;

Figure 5-35 Setting reference range

4. Press [F2] to save the changes. If the changes are successfully saved, a message box

shown in Figure 5-36 will pop up; CLICK the “Yes” to close the message box;

Figure 5-36 A message box to note data saved

5. If some entered limits are invalid, a message box shown in Figure 5-37 will pop up.

CLICK “Yes” and enter valid number again;

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Customizing the Analyzer Software

Figure 5-37 A message box to remind you of the erroneous entry

6. If you want to resume the default settings, press [F4].

 Exiting the “Ref. Range” group

When you have finished changing all the parameter units and count time settings you want to

change, you may

1. Press [F1] to select another setting group you want to change; or;

2. Press [MENU] to exit to the system menu.

5.3.7 Auto Maintain

You can select “Auto Maintain” group to view or set the how many WB samples can be run

after perform auto maintaining.

 Selecting “Auto Maintain” group Press [F1] to select the “Auto Maintain” group.

Figure 5-38 “Auto Maintain” screen(1)

The screen displays:

1) how many WB samples had been run after auto-cleaning and how many WB

samples can be run between two auto-cleanings;

2) how many WB samples had been run and how long the analyzer run after soak by

probe cleanser

5-23

Customizing the Analyzer Software

3) how many WB samples had been run after probe wipe cleaning.

For more information about auto maintain, see Chapter 10 Maintaining Y our Analyzer for

detail.

 Change the WB samples can be run between two cleanings

1. Enter the administrator password as introduced in Chapter 5.2.1.

2. At the “Settings” screen, press [F1] to select the “Auto Maintain” group, as Figure 5-39

shows.

3. ENTER the desired digits.

Figure 5-39 Auto maintain screen(2)

 Exiting the “Auto Maintain” group

When you have finished the changing, you may

1. Press [F1] to select another setting group you want to change; or;

2. Press [MENU] and a message box will pop up to remind you to save the changes, as

Figure 5-40 shows. CLICK “Yes” to save the changes and exit to the system menu; or

CLICK “No” to exit to the system menu without saving the changes.

Figure 5-40 Saving changes

5-24

Customizing the Analyzer Software

5.3.8 Other Settings

You can select the “Other” group to view or change other settings.

 Selecting the “Other” group Press [F1] to select the “Other” group.

 Muting beeper

This analyzer beeps when an error occurs. You can mute the beeper by pressing any key or

leave it beeping until the errors are removed. If you prefer the former, SELECT “Enabled”

from the “Any key to mute” drop-down list ; if you prefer the latter, SELECT “Disabled” from the “Any key to mute” drop-down list. See Figure 5-41 shows.

Figure 5-41 Selecting how to mute the beeper

 Selecting LCD contrast

Follow the steps given below to adjust the LCD contrast.

1. SELECT “LCD contrast”, as Figure 5-42 shows;

2. ENTER the desired contrast (0 to 255).

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Customizing the Analyzer Software

Figure 5-42 Select LCD contrast

 Setting alarm time

Follow the steps given below to set for how long (2s to120s) the error messages listed in

Table 5-4 should be displayed on the screen.

Table 5-4 Error List

No. Error No. Error No. Error

1 Communication Error 2 Scanner Error 3 Scanner

Communication Error

4 Environmental

5 Background Abnormal 6 HGB Error

Temperature Abnormal

7 HGB Adjustment 8 WBC Clog 9 WBC Bubbles

10 RBC Clog 11 RBC Bubbles

1. SELECT “Alarm time(s)”, as Figure 5-43 shows;

2. ENTER the desired digits.

5-26

Customizing the Analyzer Software

Figure 5-43 Setting alarm time

 Selecting PMB color (administrator password needed)

The PMB color refers to the background color of the screen when your analyzer is in the

prediluted mode. Follow the steps below to select the PMB color.

1. Enter the administrator password as introduced in Chapter 5.2.1;

2. At the “Settings” screen, press [F1] to select the “Other” group;

3. SELECT “Black” (default) or “Blue” from the “PMB color” drop-down list, as Figure

5-44 shows.

Figure 5-44 Setting PMB color

 Delete data information list

You can delete the information in the list of “Dept.”, “Sender”, “Tester” and “Checker” stored

5-27

Customizing the Analyzer Software

in the edit window when input patient information.

1. Enter the administrator password as introduced in Chapter 5.2.1;

2. At the “Settings” screen, press [F1] to select the “Other” group;

3. SELECT “Not Delete” (default) or “Delete All” from the “Info. In list of Dept., Sender

etc.” drop-down list, as Figure 5-45 shows.

Figure 5-45 Delete the information

 Exiting the “Other” group

When you have finished changing all the parameter units and count time settings you want to

change, you may

1. Press [F1] to select another setting group you want to change; or;

2. Press [MENU] and a message box will pop up to remind you to save the changes, as

Figure 5-46 shows. SELECT “Yes” to save the changes and exit to the system menu; or SELECT “No” to exit to the system menu without saving the changes.

5-28

Customizing the Analyzer Software

Figure 5-46 Saving changes

5-29

6 Operating Your Analyzer

6.1 Introduction

This chapter provides step-by-step procedures for operating your analyzer on a daily basis.

Initial Checks

Power on

Daily Quality Control

Sample Collection and Handling

Whole Blood Mode？

Yes

Run Whole Blood Samples

Shutdown

Run Prediluted Samples

6-1

Operating Your Analyzer

6.2 Initial Checks

Perform the following checks before turning on the analyzer.

1. Check and make sure the waste container is empty;

2. Check and make sure there are enough reagents;

z Samples, controls, calibrator and waste are potentially infectious. Wear

proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.

z Dispose of reagents, waste, samples, consumables, etc. according to

government regulations.

3. Checking tubing and power connections ;

 Check and make sure the diluent, rinse and waste tubes are properly connected and not

bent;

 Check and make sure the power cord of the analyzer is properly plugged into an

electrical outlet.

4. Checking the printer (optional) and recorder;

Check and make sure enough printer or recorder paper is installed. Check and make sure the

power cord of the printer is properly plugged into an electrical outlet. Check and make sure

the printer cable is properly connected to the analyzer.

5. Check keyboard connection.

Check and make sure the keyboard is properly connected to the keyboard interface (marked

“KB”) of the analyzer.

6-2

Operating Your Analyzer

6.3 Power-on

Place the power switch at the back of the analyzer in the ON position (1) to turn on the

analyzer. The power indicator light will be illuminated and the screen will display

“Initializing…“.

The analyzer will sequentially initialize the file, hardware and fluidic systems and the whole

initializing process lasts 4 to 7 minutes, depending on how the analyzer was previously shut

down.

If any error occurs during the initialization, the analyzer will display the error messages in the

lower left corner of the screen. You should remove all the errors before running any sample.

See Chapter 11 Troubleshooting Your Analyzer for solutions.

z Running samples with the abnormal background error present will lead to

unreliable results.

6-3

Operating Your Analyzer

6.4 Daily Quality Control

Before running any samples, run the controls. See Chapter 8 Using the QC Programs for

details.

6-4

Operating Your Analyzer

6.5 Selecting Analysis Mode

At the “Count” screen, press [MODE] to select one of the six analysis modes. The selected mode will be displayed in the Count Mode area.

1. Whole - All

It means the sample to be analyzed is a whole blood sample and all the 19 parameters are to

be analyzed.

2. Whole - WBC/HGB

It means the sample to be analyzed is a whole blood sample and only the following

parameters are analyzed: WBC, Lymph#, Mid#, Gran#, Lymph%, Mid%, Gran% and HGB,

plus the WBC histogram.

3. Whole -RBC/PLT

It means the sample to be analyzed is a whole blood sample and only the following

parameters are analyzed: RBC, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT, MPV,

PDW and PCT, plus the RBC and PLT histograms.

4. Prediluted - All

It means the sample to be analyzed is a prediluted blood sample and all the 19 parameters

are to be analyzed, plus 3 histograms.

5. Prediluted - WBC/HGB

It means the sample to be analyzed is a whole blood sample and only the following

parameters are analyzed: WBC, Lymph#, Mid#, Gran#, Lymph%, Mid%, Gran% and HGB,

plus the WBC histogram.

6. Prediluted -RBC/PLT

It means the sample to be analyzed is a prediluted blood sample and only the following

parameters are analyzed: RBC, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT, MPV,

PDW and PCT, plus the RBC and PLT histograms.

6-5

Operating Your Analyzer

6.6 Sample Collection and Handling

z Samples, controls, calibrator and waste are potentially infectious. Wear

proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.

z Avoid direct contact with blood samples.

z Do not re-use disposable products.

z Use clean K

test tubes and 20µL borosilicate

EDTA anticoagulant collection tubes, fused silica glass/plastic

glass capillary tubes.

6.6.1 Whole Blood Samples

Collect and handle the whole blood sample as follows:

1. Collect venous blood with a K

2. Rapidly and thoroughly mix the blood with the anticoagulant.

EDTA (1.5 to 2.2mg/mL ) anticoagulant collection tube;

z You should collect at least 2mL venous blood sample. z For the whole blood samples to be used for WBC differential or PLT count,

you shall store them at the room temperature and run them within 4 hours after collection.

z If you do not need the PLT, MCV and WBC differential results, you can store

the samples in a refrigerator (2℃ to 8℃) for 24 hours. You need to warm the

6-6

Operating Your Analyzer

refrigerated samples at room temperature for at least 30 minutes before running them.

z Mix any sample that has been prepared for a while before running it.

6.6.2 Prediluted Samples

Collect and handle the prediluted sample as follows:

1. Press [MENU] and SELECT ”Count” to enter the ”Count” screen;

2. Press [MODE] to select prediluted analysis mode (any mode preceded by Prediluted);

3. Press [DILUENT] and a message box will pop up to instruct you how to dispense the

diluent into the sample tube, as Figure 6-1 shows;

Figure 6-1 A message box showing you how to dispense diluent

4. Present a clean sample tube to the sample probe and make sure the tube is tilted

towards the probe, as Figure 6-2 shows, to avoid spills and bubbles. Press the aspirate

key to dispense 0.7mL of diluent (the dispensing volume is controlled by the analyzer)

into the tube;

Figure 6-2 How to dispense diluent

5. When the dispensing is finished, press [ENTER] to close the message box;

6. Add 20µL of capillary blood to the diluent and shake the tube to mix the sample.

6-7

Operating Your Analyzer

z Keep dust from the prepared diluent. z When preparing a prediluted sample, only use lint-free tissue to wipe the

external wall of the capillary tube; do not use cotton balls.

z After mixing the capillary sample with the diluent, wait 5 minutes before

running the sample.

z Run the prediluted samples within 30 minutes after the mixing. z Mix any sample that has been prepared for a while before running it. z Evaluate predilute stability based on your laboratory’s sample population

and sample collection techniques or methods.

6-8

Mindray BC-2800 Operator's Manual

Specifications and Main Features

Frequently Asked Questions

User Manual