Leica DM6000 CFS
Confocal Fixed Stage System
The solution for applications in live cell research, from physiology to electrophysiology – from single cells to whole organisms
Leica TCS SP5 with integrated fixed stage microscope Leica DM6000 CFS
Features and Benefits
•Maximum workspace for manipulators and attached pipettes
•High collection efficiency due to short collection path before detectors
•Integrated change of magnification (0.35x, 1x, 4x) of camera port
•Remote control of all microscope functions via touch panel
•Special booster optic to fill the entrance pupil of the 20x1.0 objective
•“Dip-in” feature for immersing the objective
•Patented condenser drainage system
•High microscope and sample stability
Excellent Results
Under All Conditions
Leica Microsystems sets a new standard with the integration of the Leica DM6000 CFS fixed stage microscope into the Leica TCS SP5 confocal platform.
Neurobiological research has a long history not only in measurements of single cells, but also of thick brain slices. Here more realistic results can be achieved due to the intact network. Ultimately, accessing the brain directly in a whole animal provides the most intact environment for conducting measurements on individual cells. So for optimal results, space for whole animal samples is an absolute must.
Electrophysiological research employs micropipette systems for recording electrical signals (patch clamp, whole cell recordings), electrical stimulation (intracellular stimulation, synaptic stimulation, soma-stimulation), dye injection and intracellular perfusion. To make such measurements effective, the microscope needs to have as much space as possible for micropipettes and other manipulators.
Searching for the perfect spot in the sample requires a large field of view, whilst the precise positioning of the micropipettes requires high magnification. Furthermore, high quality confocal image scanning requires a large numerical aperture for best performance. To fulfill these experimental prerequisites, a camera with an adjustable magnification and specially adapted objectives are needed. Any direct manipulation of the system potentially disturbs the delicate positioning of the micropipettes within the sample. This emphasizes the importance of a remote control for the imaging setup, providing convenient access to all relevant functions.
Finally, recording electrophysiological and imaging data with perfect synchrony is paramount for correct interpretation. Triggering image recording by external events and synchronizing the application of stimuli with image scanning down to the single line level helps realize sophisticated experimental setups. Online analysis of the resulting data allows on-the-fly adjustment of external parameters, helping the researcher to get everything just right.
The Leica TCS SP5 with the integrated fixed stage microscope Leica DM6000 CFS gives excellent experimental results under all conditions.
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Variable Field
at High Resolution
With the highest aperture objective 20x 1.0 NA, with the innovative, ergonomic and stable microscope stand Leica DM6000 CFS, with short coupled non-descanned detectors and with the fastest, most sensitive scanning system SP5, we enter a new dimension in imaging, from single cells to whole organisms.
The objective
The new Leica HCX APO L 20x 1.0 water immersion objective offers both a large field and a high resolution with one single objective lens, making it unnecessary to change objectives between overview imaging for sample preparation and detail imaging for data recording.
In addition to optimizing the optical parameters, a number of physical, chemical and mechanical improvements have been integrated into the new HCX APO L 20x1.0 application objective: It is corrosion-free, chemically neutral and avoids diffusion of metal ions. Wettability is outstanding, thermal conductivity is minimal and magnetic fields at the front of the objective have been eliminated.
These new objective characteristics are possible by employing a special, extremely hard ceramic material for the whole front lens area. This material is resistant to mechanical damage and much better suited to the requirements of electrophysiology than typical objective metal sleeves.
A further advantage is the access angle of the objective, which is a measure of how easily manipulators can be fitted. This has been widened to 39 degrees, which is almost the limit of what is theoretically possible. In combination with a free working distance of 2 mm, the 20x objective is ideal for many applications.
Leica objective HCX APO L 20x1.0
Transmission HCX Apo L 20x/1.00 W
Features and Benefits
•20x water immersion objective
•High numerical aperture (NA) of 1.0
•Free working distance (FWD) of 2 mm
•Optimized access angle of 39°
•Large objective focusing range of 13 mm
•Insulated ceramic tip
•Minimum conductivity
•High transmission in VIS and IR (see graph)
•Best DIC and Dodt contrast
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Leica DFC350 FX Camera with parfocal magnification changer
Features and Benefits
of the Leica STP6000
•Intuitive user interface
•Control of all microscope functions
•Z-focusing wheel for coarse and fine z-movements
•Freely programmable function keys
•Helps to avoid vibrations caused by manual operation
The microscope stand
But an objective alone doesn’t make an electrophysiology setup. The Leica DM6000 CFS combines the specific needs of electrophysiological experiments with the optimized imaging performance of the DM series. With its focusing objective or nosepiece, it offers a highly stable and convenient optical platform for all fixed stage applications.
To cover the demand for large scale sample screening and fine needle approaches for electrophysiology, the DM6000 CFS has a special observation tube with 3 magnification positions (0.35x; 1x; 4x). This parfocal magnification changer allows a total change in magnification up to a factor of 12 without changing the objective.
The specimen is completely decoupled from the microscope, both mechanically and electrically. The microscope provides a high degree of free space for application-specific sample holders, bath chambers and several manipulators at a time. In this way, the sample and the patch electrodes can be moved below the fixed optical axis of the microscope. This allows the researcher to scan the entire dendritic tree and follow the axons of fluorescently labeled cells. The DM6000 CFS is also designed for use with a separate third-party fixed platform for holding micromanipulators and other devices, as well as an x,y-translation stage.
To avoid the need for direct manipulation of the microscope stand which could cause accidental vibrations, the external control panel Leica STP6000 (Smart Touch Panel) controls all the microscope functions. A touch screen and freely programmable function keys allow quick and easy operation without adversely affecting the measurements.
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