Leica ASP 200 S V1.1 Manual

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Leica ASP200S

Advanced Smart Processor

Automated vacuum tissue processor

Operating Manual

Leica ASP200 S

V1.1 English - 03/2009

Always keep this manual together with the instrument. Read this instruction manual carefully before working with the instrument.

NOTE

The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field.

We are under no obligation to update the present manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.

For erroneous statements, drawings, technical illustrations etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual.

Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products.

Published by:

Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19

D-69226 Nussloch Germany

Telephone: +49 (0)6224 143-0

Fax: +49 (0)6224 143-200

Internet: http://www.histo-solutions.de

These are determined only by the contract provisions agreed between ourselves and our customers.

Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products. This document is protected under copyright laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument.

© Leica Biosystems Nussloch GmbH

Leica ASP200 S

3

Table of Contents

 

1.

Important information .................................................................................................................

6

2.

Safety ..............................................................................................................................................

7

2.1

Safety instructions....................................................................................................................................

7

2.2

Warnings ....................................................................................................................................................

7

2.3

Integrated safety devices ......................................................................................................................

11

3.

Instrument components and specifications .........................................................................

12

3.1

Overview - instrument components.....................................................................................................

12

3.2

Specific instrument options ..................................................................................................................

14

3.3

Standard delivery - packing list ............................................................................................................

15

3.4

Technical Data ........................................................................................................................................

16

3.5

Compatible reagents ..............................................................................................................................

19

4.

Setting up the instrument .........................................................................................................

20

4.1

Site requirements ...................................................................................................................................

20

4.2

Electrical connection .............................................................................................................................

20

4.3

Installing the accessories .....................................................................................................................

21

4.4

Setting up the data connections ..........................................................................................................

23

4.5

Anti-reflection clip – Installation Instructions....................................................................................

24

4.6

Alarm functions .......................................................................................................................................

25

4.7

Switching the instrument on .................................................................................................................

26

4.8

Touchscreen functions ..........................................................................................................................

28

4.9

Checklist for first operation ...................................................................................................................

29

4.10

Switching of the instrument ..................................................................................................................

30

5.

Operation .....................................................................................................................................

31

5.1

Setting up the instrument parameters.................................................................................................

31

5.1.1

System setup ...........................................................................................................................................

31

5.1.2

Access levels ..........................................................................................................................................

34

5.1.2

INSTALLATION menu .............................................................................................................................

36

5.1.3

Editing the reagent list ...........................................................................................................................

39

5.1.4

Viewing the program list ........................................................................................................................

42

5.1.5

Adding and/or modifying programs .....................................................................................................

43

5.1.6

Favorites ...................................................................................................................................................

46

5.1.7

Editing the stations .................................................................................................................................

48

5.1.8

Reagent groups .......................................................................................................................................

49

4

Operating Manual V 1.1 – 03/2009

 

 

Table of Contents

5.2

Reagent handling ....................................................................................................................................

50

5.2.1

Fill/drain reagents (other than wax).....................................................................................................

50

5.2.2

Changing the wax ...................................................................................................................................

52

5.3

Running programs ..................................................................................................................................

55

5.3.1

Editing a selected program ...................................................................................................................

56

5.3.2 Starting a program..................................................................................................................................

58

5.4

Reagent status ........................................................................................................................................

59

5.5

System Monitor .......................................................................................................................................

60

5.6

On-line help..............................................................................................................................................

61

6.

Solutions......................................................................................................................................

62

6.1

General .....................................................................................................................................................

62

6.2

Power failure ...........................................................................................................................................

62

6.3

Troubleshooting ......................................................................................................................................

63

6.4

Typical fill or drain problems.................................................................................................................

64

7.

Cleaning and Maintenance .....................................................................................................

65

7.1

Clean programs .......................................................................................................................................

65

7.1.1

Retort clean programs ...........................................................................................................................

65

7.1.2

Wax clean program ................................................................................................................................

67

7.1.3

Smart Clean .............................................................................................................................................

68

7.2

General cleaning steps ..........................................................................................................................

69

7.3.1

Daily cleaning and maintenance ..........................................................................................................

71

7.3.2

Periodic cleaning and maintenance ....................................................................................................

73

7.4

Preventative maintenance schedule ...................................................................................................

74

8.

Warranty and Service ...............................................................................................................

76

9. EC Declaration of Conformity ..................................................................................................

77

Leica ASP200 S

5

1.Important information

Symbols used in this manual

Warnings

appear in a gray box and are marked by a warning triangle .

Notes,

i.e. important user information appear in a gray box and are marked

by an information symbol .

Flammable solvents and reagents are marked with this symbol.

Instrument surfaces which become hot during operation are marked with this symbol.

Avoid direct contact with these surfaces - they may cause burns.

Figures in brackets refer to item

(5)nos. in drawings.

Function keys to be pressed on the ENTER instrument touch screen are written

in bold-print capital letters.

Qualification of personnel

The Leica ASP200 S may be operated only by trained laboratory personnel.

The instrument may be operated only according to the instructions contained in this manual.

Designated use

The instrument has been designed so that it is safe to use by the operator as well as for processing specimens – provided that it is operated according to the present instruction manual.

The Leica ASP200 S is a modular automated tissue processor designed for the following laboratory applications:

fixation,

dehydration

paraffin wax infiltration of histological tissue specimens.

The Leica ASP200S must be operated exclusively with the reagents listed

in chapter 3.5 - "Compatible reagents" .

Any other use of the instrument is considered improper!

Failure to adhere to these instructions may result in an accident, personal injury, damage to the instrument or accessory equipment.

Instrument type

All information provided in this manual applies only to the instrument type indicated on the title page.

A name plate

 

indicating the

 

instrument

 

serial number

 

is attached to

 

the back of the

 

instrument.

Fig. 1

6

Operating Manual V 1.1 – 03/2009

2. Safety

Make sure to comply with the safety instructions and warnings in this chapter.

Make sure to read these instructions, even if you are already familiar with the operation and use of other Leica products.

2.1 Safety instructions

This instruction manual includes important information related to the operating safety and maintenance of the instrument and it is an important part of the product.

If additional requirements on accident prevention and environmental protection exist in the country of operation, this instruction manual must be supplemented by appropriate instructions to ensure compliance with such requirements.

This instrument has been built and tested in accordance with the safety regulations on electrical measuring, control, regulating and laboratory devices.

In order to maintain this condition and to ensure safe operation the operator must comply with the instructions and warning contained in this instruction manual.

For current information on applicable standards, please refer to the instrument’s CE declaration and visit:

http://www.histo-solutions.com

The protective devices on both instrument and accessories may neither be removed nor modified. Only authorized and qualified service personnel may repair the instrument and access the instrument’s internal components.

2.2Warnings

The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Primarily responsible for accident-free operation is above all the institution which owns the instrument and, in addition, the designated personnel who operates, services or repairs the instrument.

To ensure trouble-free operation of the instrument, make sure to comply with the following instructions and warnings.

Leica ASP200 S

7

2.Safety

Warnings - Markings on the instrument itself

Markings on the instrument showing the warning triangle indicate that the correct operating instructions (as defined in this manual) must be followed when operating or replacing the item marked. Failure to adhere to these instructions may result in an accident, personal injury, damage to the instrument or accessory equipment.

Some instrument surfaces become hot during operation.

They are marked with this warning label. Touching these surfaces may cause burns.

Transport and Installation

The instrument may only be transported in an upright position.

Follow the unpacking instructions carefully to avoid damage to the instrument!

A cleaning must be performed prior to each transport, during which the instrument may be shaken, tilted or lifted. Severe internal damage may otherwise occur.

The instrument MUST be connected to an earthed mains power outlet socket. The instrument must not be connected to an extension cord without protective earth conductor. Ensure uniform current supply according the to set voltage!

The set voltage CANNOT be changed by the user.

Severe damage may occur if the instrument is connected to a power supply voltage other than that to which it was originally set.

The instrument must be set up in a well ventilated area, free from any ignition sources. The chemicals to be used in the Leica ASP200 S are both flammable and noxious.

Do not operate the instrument in rooms with explosion hazard.

If there is a significant difference in temperature between the warehousing and the installation site of the instrument and if at the same time there is a high air humidity level, condensation water may form. In this case, a waiting period of at least two hours must be observed before the instrument is switched on. Failure to adhere to this waiting period may result in damage to the instrument. Failure to adhere to this waiting period may result in damage to the instrument.

8

Operating Manual V 1.1 – 03/2009

2. Safety

Warnings - Operating the instrument

The Leica ASP200S may only be operated by trained laboratory personnel, according to its designated use and per the present instruction manual.

The ON/STOP switch at the side of the instrument may be used in an emergency to stop the instrument during operation.

Always press PAUSE before opening the retort lid during processing to allow the retort to vent.

Do not remove the wax drain hose or the remote fill/drain hose until the fill or drain has been completed, as pressurized air is used to clear the hose after each fill / drain.

After refilling / replacing the reagent bottles ensure that the lids are tight.

The bottles must be properly pushed home into the connection manifolds at the rear inner wall of the reagent module.

Failure to correctly plug the reagent bottles into the manifold will cause an interruption to the processing run and may result in spilling of reagents.

Fixatives containing mercuric salts, acetic or picric acid will corrode metallic components in the instrument.

After each paraffin step a retort clean cycle must be run.

Material safety data sheets can be obtained from the supplier of the chemicals. They are also available on the Internet:

http://www.msdsonline.com

Leica ASP200 S

9

2.Safety

Warnings -handling reagents

Be careful when handling solvents!

Always wear rubber gloves and safety goggles when handling the chemicals used in this instrument.

Reagents used for tissue infiltration can be both toxic and/or flammable.

To avoid damaging the instrument only use those reagents that are listed in chapter 3.5! Do not use acetone, benzene or trichlorethane in the instrument!

Use caution when handling paraffin wax or removing baskets – molten paraffin is hot and may cause burns.

Also, avoid personal contact with paraffin stations and retort walls – they can be very hot as well.

Dispose of waste solvents with care according to local regulations and the waste management policy of the company or institution.

Do not clean reagent bottles in an automatic dishwasher – they are NOT dishwasherproof.

Warnings - Cleaning and maintenance

Prior to each maintenance and/or cleaning, switch the instrument off and disconnect mains power.

Do not clean the instrument with solvents containing acetone or xylene. No liquid may be spilled into the internal components of the instrument – neither during operation nor during cleaning.

When working with cleaning detergents, comply with all safety instructions by the manufacturer of the product and the laboratory management policy.

The condensate bottle should be inspected at least once every week and, if necessary, emptied.

10

Operating Manual V 1.1 – 03/2009

2. Safety

2.3 Integrated safety devices

In the case of power failures or other processing problems the Leica ASP200S incorporates specimen protection features such as a fluid level sensor and sophisticated software controls which ensure that processing can be completed successfully without damage to the tissue specimens.

Overpressure protection

When power is off the air pump and air valves default to a safe condition (retort vented, no pressure generation).

If the microprocessor control fails to stop the air pump at the right moment during retort pressurizing, a separate electronic hardware circuit will cut pump power.

In addition, there is a safety relief valve that vents all excess air pump output to atmosphere.

Overcurrent protection

Overcurrent conditions are protected against by both the mains fuse and the separate heating power fuses.

Overheating protection

An error is indicated and all heating is stopped by the microprocessor control if the instrument detects any of the following conditions:

abnormally high temperature (>75 °C)

contradictory results of the temperature sensors

failure of one or more heating power control components

If the microprocessor fails to interrupt heating power, independent temperature limiting hardware circuits limit the temperature rise to a safe level.

If the temperature limiting circuits malfunction, an independent hardware thermal fuse circuit cuts power to the heating elements.

Over vacuum protection

The vacuum system is not capable of generating a dangerous vacuum condition.

Overcurrent protection

Overcurrent conditions are protected against by both the mains fuse and the separate heating power fuses.

Leica ASP200 S

11

Leica ASP 200 S V1.1 Manual

3.Instrument components and specifications

3.1 Overview - instrument components

3

4

 

 

 

6

 

 

24

1

 

 

 

7

10

9

22

 

 

 

5

 

 

 

23

 

 

 

20

12

11

8

 

21

13

17

18

19

25

2

15

14

16

Fig. 2

12

Operating Manual V 1.1 – 03/2009

3.Instrument components and specifications

Overview - instrument components

1

-

Basic instrument - processor module

2

-

Basic instrument - reagent module

3

-

Wax bath lids

4

-

Retort lid

5

-

Touch screen

6

-

Cover flap of the instrument console

7

-

Instrument console comprising:

8

-

Printer port

9

-

Local alarm connection

10

-

Remote alarm connection

11

-

Floppy disk drive

12

-

Serial port

13

-

Activated carbon filter

14

-

Condensate bottle

15

-

Reagent bottle (13 units)

16

-

Drip tray

17

-

Wax drain connection

18

-

Remote drain connection

19

-

Retort

20

-

Wax baths

21

-

Specimen basket

22

-

Lid for specimen basket

23

-

Specimen basket carrier

24

-

Splash protection

25

-

Displacement block

Instrument components and accessories

The processor module contains three paraffin wax baths, a processing retort, and also a touch screen with integrated key symbols as well as the electronic components.

The cassettes are held in three baskets (21), each of which has a capacity of up to 100 cassettes.

All processing occurs in the stainless steel retort under the pressure, vacuum and temperature conditions selected.

The quick-connect reagent bottles are stored in the reagent cabinet.

Leica ASP200 S

13

3.Instrument components and specifications

3.2Specific instrument options

Reagent Management System option (RMS) that displays the usage (length and frequency of use) of each reagent and also allows automatic ordering of reagent sequence, thus eliminating the need to physically move reagent bottles. After renewal of one or more reagents in a sequence, the RMS automatically uses the reagents in order of increasing cleanliness.

In-process fluid recirculation ("tidal agitation") for continuous and efficient mixing of fluids.

Remote fill/drain system – can drain and refill reagent bottles from bulk containers via the retort and a hose plugged into the processor module while the user is fully protected against coming into contact with reagents.

Remote wax bath fill/drain feature.

Optical fluid level sensor.

Active paraffin wax solvent removal feature that increases the life of the wax by extracting and condensing contaminating solvents.

Magnetic stirrer – to gently circulate reagents, ensuring uniform reagent temperature.

Programmable finish time for processing programs.

Three-step retort drain feature (adjustable) minimizes reagent carryover.

Specimen processing under pressure, vacuum, alternating pressure / vacuum cycles or under ambient pressure.

Four user-programmable clean programs. The clean programs automatically skip any steps not required to complete the clean.

14

Operating Manual V 1.1 – 03/2009

3.Instrument components and specifications

3.3 Standard delivery - packing list

The basic version of the Leica ASP200 S includes the following parts:

Part no.

1Leica ASP200 Basic Instrument

1Set of power cords

 

230-240 V version:

 

 

1

Mains cable "D"

14 0411 13558

 

1 Mains cable "UK" ST-BU F-5A

14 0411 27822

 

100-120 V version:

 

 

1

Mains cable "USA-C-J"

14 0411 13559

1

Jumper cable - mains

14 0411 34604

1

ASP300 S basket set, consisting of:

 

 

1 Basket carrier

14 0480 43578

 

2 Specimen baskets with integrated spacers

14 0476 43569

 

1 Cover for first basket

14 0476 43362

1

Displacement block

14 0480 37127

1

Remote fill/drain hose

14 0476 34716

1

Paraffin drain hose

14 0476 34721

1

Funnel

14 0476 43631

1

Lubricant, Molykote 111, for valves and O-rings

14 0336 35460

2

Activated carbon filters, assy.

14 0476 34150

1

Magnetic stirrer, complete

14 0476 34202

1

Paraffin wax scraper

14 0476 35923

14

Reagent bottles, plastic (13 in instrument)

14 0476 34274

1

Condensate collector, plastic (in instrument)

14 0476 34278

1

Drip tray (in instrument)

14 0476 34145

1

Splash protection

14 0476 34770

1

Maintenance kit (2 replacement lids, 9 o-rings)

14 0476 35921

1

Maintenance kit for pump (in instrument)

14 0476 35922

2

Sets of labels for reagent bottles, set of 15 each

14 0476 35792

1

Remote alarm plug

14 6844 01005

1

Operating manual for Leica ASP200 S, multi-language

14 0480 80001

1

3,5" HD-diskette, blank, DOS-formatted, (on rear of console cover)

 

Please check all delivered parts against the packing list and against your order to verify whether the delivery is complete! If there is any difference, please contact your local Leica Sales Office immediately.

Leica ASP200 S

15

3.Instrument components and specifications

3.4 Technical Data

Nominal voltage:

Nominal frequency:

Main fuses:

Nominal power:

Dimensions, (W x D x H), in mm: Dry weight, unpacked: Weight, packed:

Operating temperature range: Relative humidity:

IEC 1010 classifications:

Altitude:

Local / remote alarm relays:

Two factory-preset voltages (not user-adjustable):

100 to 120 V

or

230 to 240 V

 

50 to 60 V

Two fast-blowing melting fuses, 20 x 5 mm, UL-approved

for 100 to 120 V ; F 10 A 250 VAC

for 230 to 240 V ; F 5 A 250 VAC

1000 VA

595 x 680 x 1,325 approx. 160 kg 220 kg

15 °C to 35 °C

10 % to 80 %, non-condensing

Protective class 1 Pollution degree 2

Overvoltage installation category II:

800 V impulse (120 V systems)

1500 V impulse (240 V systems)

2500 m maximum

30 V DC, maximum 2 A

2 connections:

Both single voltage-free, changeover contacts (both Normally-Open and Normally-Closed connections available)

Paraffin wax baths

 

Number of baths:

3

Volume (liters):

4.3 l per bath

Melt time:

approx. 10 hrs

Temperature:

40 to 65 °C

Temperature accuracy:

+ 1 K

16

Operating Manual V 1.1 – 03/2009

 

3.

Instrument components and specifications

 

 

 

Retort

 

 

Capacity:

 

max. 250 cassettes

Reagent volume (in liters):

4.3

Temperature (paraffin):

40 to 65 °C

Temperature (processing reagents):

ambient or 35 to 55°C

Temperature (cleaning reagents):

50 to 65 °C

Temperature accuracy:

+ 1 K

Filling time:

approx.

90 sec

Drain time:

approx.

80, 120, 140 sec (adjustable)

Impregnation vacuum:

-70 kPa (g)

Impregnation pressure:

35 kPa (g)

Fill vacuum:

 

-70 kPa (g)

Drain pressure:

35 kPa (g)

General

 

 

Reagent bottles:

10

Cleaning solution bottles:

3

Maximum bottle volume:

4.3 l

Printer:

 

Optional accessory

Pretest check:

ON/OFF

Fluid level sensor:

ON/OFF

Recirculation (pump in/out):

ON/OFF

(a) Time before 1st cycle:

16 min

(b) Time between cycles:

20 min

System setup

 

Password status:

Supervisor / User

Type of password:

alphanumeric, freely selectable

Reagent Management Status:

ON/OFF

Software access control (lock mode):

ON/OFF

Leica ASP200 S

17

3.Instrument components and specifications

3.4 Technical Data (continued)

Hardware and Software:

Large color Liquid Crystal Display (LCD) and touchscreen.

User-friendly, intelligent software.

3,5" floppy drive and printer port.

Alarm system with two remote alarm sockets.

Password-protected instrument supervisor mode.

Multiple specimen protection system.

Capacities:

• 15 programs that consist of up to 10 reagent and 3 paraffin wax steps.

Time per program step:

0 to 99 hours, 59 minutes.

Delay time:

max. 7 days.

Up to 250 cassettes can be processed simultaneously.

Four user-programmable retort clean programs.

Wax clean program.

10 reagent bottles.

3 wax baths.

3 cleaning solution bottles.

1 condensate bottle.

Reagent temperature adjustable from 35 °C to 55 °C or ambient.

Paraffin temperature adjustable from 40 °C to 65 °C.

Choice of three retort drain rates of 80, 120 and 140 seconds.

Up to 100 reagent names in memory.

18

Operating Manual V 1.1 – 03/2009

3.Instrument components and specifications

3.5 Compatible reagents

The following reagents may be used in the Leica ASP200S:

Fixatives

1.Formalin, buffered or unbuffered

2.Formalin saline

Dehydration

1.Ethanol

2.Isopropanol

3.Methanol

Clearing

1.Xylene

2.Toluol *

3.Chloroform *

Paraffin wax 1. Paraffin

4.Butanol

5.Industrial Methylated Spirits

* Prior to using these reagents, contact your Leica representative or Leica directly for informationonnecessaryprotectivemeasures.

Caution:

Reagents other than those listed here may damage some components of the instrument.

Do not use acetone, benzene or trichlorethane in the instrument.

Fixatives containing mercuric salts, acetic or picric acid will corrode metallic components in the instrument and shorten instrument life.

If you choose to work with such fixatives, it is essential to perform a clean cycle which contains multiple water rinses each time after use, to minimize damage.

In addition, we recommend frequent and regular preventive maintenance by the Leica Technical Service.

Leica ASP200 S

19

4.Setting up the instrument

4.1 Site requirements

The instrument must be set up in a clear space of about 650 x 700 mm on a non-vibrating base.

Room temperature constantly between +10°C and +35 °C.

Relative humidity maximum 80%, non-con- densing.

Avoid vibrations, direct sunlight and heavy variation in temperature.

Moving the instrument

The chemicals to be used in the Leica ASP200S are both flammable and noxious. The Leica ASP200S must be set up in a well ventilated area free from any ignition sources. Do not operate the instrument in rooms with explosion hazard.

When the instrument has been unpacked (see Unpacking instructions on the outside of the shipping container), it must be grasped only at the points indicated by ‘‘ to move it to its final setup location. Once it has reached this position, the brakes on the instrument rollers must be engaged immediately.

4.2 Electrical connection

Caution!

The instrument MUST be connected to an earthed mains power outlet socket.

It is recommended that the ASP200S be plugged into a wall socket that has Ground Fault Circuit Interruption (GFCI) protection – as an additional electrical safeguard.

Make sure to use the appropriate mains cable for the local voltage supply (wall outlet).

The instrument must be set up so that the power switch on the rear of the instrument (item 42 in fig. 4) can be reached at all times.

Check the voltage label (Fig. 3) on the rear of the instrument to ensure that the instrument delivered is set to the correct voltage range.

Severe damage may occur if the instrument is connected to a power supply voltage other than that to which it was originally set.

The power supply voltage for the instrument is factory preset, and CANNOT be altered by the user.

Fig. 3

20

Operating Manual V 1.1 – 03/2009

4.Setting up the instrument

Connecting the instrument to mains

41

42

40 43

Illustration on the left Electrical connections at the rear panel of the instrument.

Once the instrument has been switched on, the mains switch (ON/OFF) (42) should always remain in position “ON“.

37

• Connect the mains cable (37) to

the mains power supply socket

 

 

(41).

 

 

• The jumper cable (38) which is

 

supplied as part of standard

 

delivery connects the mains

 

power supply output (43) to the

38

electronics module input (40).

 

 

 

 

 

 

 

Important!

 

 

The specification for

 

 

connection (40) is as

Fig. 4

 

follows:

Illustration on the right

 

100 - 120 V or

Correct connection of the

 

230 - 240 V,

cables

 

maximum 200VA.

 

 

 

4.3 Installing the accessories

 

• Place the instrument in the definitive installa-

 

 

tion position.

13

18

Activated carbon filter

 

 

29

 

• Unpack the activated carbon filter (29) and in-

 

 

 

 

sert it into the instrument (see Fig. 5).

 

 

Make sure to insert the filter with the correct

 

 

side facing up-

 

 

wards. See label

 

 

(27) at the front of

 

 

the filter – the ar-

 

 

row must point

 

 

upwards.

 

 

 

 

Fig. 5

Leica ASP200 S

21

4.Setting up the instrument

28

18

Fig. 6

31

30

32

Fig. 7

Remote fill / drain hose

Connect the supplied remote fill/drain hose to the remote drain connection on the front of the instrument (see Fig. 6).

Important!

When inserting the hose into the fill/drain connection (item 18 in Fig. 6) the connecting device (28) of the hose must lock with an audible click.

If bulk containers can be located next to the instrument during remote filling and draining, the hose may be shortened to improve handling and the fill/drain rate.

If the hose is shortened, a vee-notch can be cut into the hose end to ensure a clear passage for fluid flow.

Magnetic stirrer

Unpack the magnetic stirrer (30, fig. 7) and insert into the retort.

Important!

Insert the magnetic stirrer so that the convex side (32) of the rotor blades (31) faces down. (Fig. 8)

Inserting the stirrer incorrectly will result in unsatisfactory stirring action.

22

Operating Manual V 1.1 – 03/2009

4. Setting up the instrument

26

25

27 25

Fig. 8

10

9

8

11

12

6

Inserting the Displacement block

Important!

The instrument must not be operated unless the volume displacement block is in the retort. Otherwise, malfunctions may cause damage to the instrument.

Grasp the volume displacement block (25) by the metal clip (26) and insert into the retort so that the hole (27) is located above the position of the magnetic stirrer.

4.4 Setting up the data connections

Printer (optional)

Lift the flap of the instrument console (6).

Connect the printer to the printer port (8) in the console via a standard Centronics cable (Fig. 9)

A suitable printer can be recommended by your Leica distributor.

If conformance to electromagnetic interference standards is essential, a specially shielded printer cable will be required.

Other connections

For additional data transfer and storage the instrument console is equipped with a standard 1.44 MB floppy disc drive (11) and an RS 232 serial interface (12).

Fig. 9

Leica ASP200 S

23

4.Setting up the instrument

4.5 Anti-reflection clip – Installation Instructions

Anti-reflection clip – Function

The anti-reflection clip (Order no. 14 0476 44135) prevents any reflections that might otherwise be emitted from the specimen basket (3, order no. 14 0476 34193) and that could affect the level sensor in the retort, thus causing malfunctions.

1 3

4

2a

Fig. 9

1

4

2b

Fig. 10

4 5

Fig. 11

Installing the clip

The anti-reflection clips are intended for installation on the specimen basket to prevent reflections of the level sensor.

Insert the left lug (2a) of the anti-reflection clip (1) into the seventh hole (from the left) of the lower oval openings (Fig. 9).

Snap the right lug (2b, Fig. 10) into the seventh hole (from the right) with light pressure.

When inserting or removing the metal baskets into/from the retort, always do so carefully so that they do not touch or become caught on the retort wall.

Changing the clip

Once the clip is installed, the handle (4) of the specimen basket cannot be removed, as otherwise the clip will be bent.

To remove or replace a clip, all parts that are inside the basket (e.g. spiral insert and separating walls) must be removed from the basket.

Then, insert a suitable tool (screwdriver 5, Fig. 11) and pry the clip out from the inside.

Once bent, clips may no longer be used and must be disposed of. In this case, install a new clip from the standard scope of delivery.

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Operating Manual V 1.1 – 03/2009

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