ÄKTApurifier
UNICORN 5.0
71-1944-37
ÄKTApurifier
ÄKTApurifier ÄKTApurifier 10 pH/C-900
ÄKTApurifier UPC 10 ÄKTApurifier UPC 100 ÄKTApurifier 100 plus
ÄKTApurifier 100 UV
ÄKTApurifier 10
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P-903 |
P-901 |
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UV-900 pH/C-900 |
ÄKTApurifier 10 plus |
ÄKTApurifier 100 plus |
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UV-900 pH/C-900 |
ÄKTApurifier 10 |
ÄKTApurifier 100 |
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UPC-900 |
ÄKTApurifier UPC 10 |
ÄKTApurifier UPC 100 |
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ÄKTApurifier 10 plus ÄKTApurifier 100 plusP-960
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ÄKTApurifier 10 |
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ÄKTApurifier 100 |
ÄKTApurifier UPC 10 |
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ÄKTApurifier UPC 100 |
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P-903 |
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P-901 |
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P-903 |
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P-901 |
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0.001 10 ml/min |
0.01 100 ml/min |
0.001 10 ml/min |
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0.01 100 ml/min |
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25 MPa |
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10 MPa |
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25 MPa |
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10 MPa |
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SV-903 *1 |
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UV |
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UV-900 |
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UPC-900 |
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190 700 nm 3 |
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280 nm 254 nm 1 |
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10 mm |
8 µl |
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2 mm |
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2 µl |
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5 mm |
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6 µl |
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2 mm |
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2 µl |
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pH/C-900 |
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UPC-900 |
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UV |
*2 |
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pH |
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*1 ÄKTApurifier 10 plus ÄKTApurifier 100 plus |
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*2 pH |
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P-901
2 0.01 100 ml/min 10 MPa
UPC-900
UV 280 nm 254 nm 1
2 mm 2 µl 5 mm 6 µl
313 365 405 463 546 nm
214 nm Zn 214 nm
pH pH
18-1027-11 10
V2 V3
V1 UV
V2 V3
i/o
V2
V3 UV
1
2 8
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Pump Wash Basic |
○ |
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Pump Wash Purifier |
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Pump Wash Explorer |
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○ |
○ |
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○ |
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カラムの接続、溶液交換など、カラムへ送液する場合に使用します。カラムへ送液する際は必ず耐圧設定を行ってか ら送液を開始します。
Manual ↓ Flowpath → ColumnPosition → → Execute
Main Selection
Wavelength and Buffer inlets
UV
UV-900 3
UPC-900
Segmented gradient advanced
Show details
UNICORN Ver.5.0
UNICORN Ver.5.0
PumpWash Purifier Continue
PumpWash PauseContinue
Frac-950
Frac-950
2
Manual↓Flowpath → OutletValve; F2→ Execute
Manual↓Frac↓Man_Fractionation → → Execute
Frac-950
Wizard Elution Fractionation
Start at First TubeSegment1 Fraction Volume
Fraction Volume 0
First Tube
→Segment Segment1 First Tube
OFF→10 ON
UV Cond/pH
Frac-900
Wizard Elution Technique Gradient
Segmented Gradient Advanced
Segmented Gradient Advanced
Contents ● ● ●
1. .......................................................................................................4
1.1 |
ÄKTApurifier ............................................................................................ |
4 |
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1.2 |
ÄKTApurifier |
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2. ...............................................................................................................9
2.1 |
UNICORN ........................................................................................................... |
9 |
2.2 |
UNICORN .......................................................................................... |
11 |
3. ..........................................................................................13
3.1 |
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3.2 |
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3.3 |
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3.4 |
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3.5 |
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PumpWash |
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3.6 |
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4. ..............................................................................................17
4.1 |
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4.2 |
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4.3 |
.......................................................................................................... |
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4.4........................................................................................ 19
5. ................................................................20 |
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5.1 ................................................... |
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5.2............................ 21
6. ................................................................22 |
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6.1 |
Frac-950....................................................................................................................... |
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6.2 |
Frac-900....................................................................................................................... |
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26 |
7. ..............................................................................................30 |
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7.1 |
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Wizard .................................................................... |
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7.2 |
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7.3 |
.......... |
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7.4 |
Equilibration ............................................................. |
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7.5 |
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7.6 |
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7.7 |
Elution .................................................................................. |
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7.8 |
Segment 1 |
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7.9 |
Segment 2 |
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7.10 |
Variables ......................................................................... |
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7.11 |
Run Setup ........................................... |
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7.12 |
.......................................................................................................... |
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7.13 |
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8. ..........................................................................................43
8.1 |
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8.2 |
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8.3 |
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8.4 |
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8.5 |
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8.6 |
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8.7 |
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8.8..................................................................... 51
9. |
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9.1 |
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9.2 |
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9.3 |
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10. ............................................................................................57
10.1 |
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10.2 |
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10.3 |
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10.4 |
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11. ............................................................................................60
11.1 |
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11.2 |
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11.3 |
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11.4 |
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11.5........................................................................................ 61
11.6.......................................................................... 61
12. |
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12.1 |
................................................................. |
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12.2 |
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Copy to External |
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63 |
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12.3 |
UNICORN CD .................................................... |
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Copy from External |
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12.5 |
.......................................................................................................... |
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66 |
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12.6 |
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Rename |
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66 |
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1
ÄKTApurifierOn-line Help
1.1ÄKTApurifier
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INV-907 |
Box-900 |
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pH/C-900 |
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PV-908 |
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UV-900 |
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M-925 |
P-903 |
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M-925 |
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(W1, W2 and W3)
A |
B |
UV |
SV-903 SV-903
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180 mm |
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New capillary130 mmof |
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New capillary of |
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130 mm |
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180 mm |
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G4 |
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pHpH |
Flow cell |
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G5 |
(Bypass) |
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On-line |
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filter |
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Column |
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G3 |
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COND |
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Flow restrictor |
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Mixer |
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G6 |
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G1 |
G2 |
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G8 |
G9 (H9) |
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(H6) |
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Injection |
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UV |
(H8) |
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Valve (V1) |
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Pump AB |
PumpBA |
21 76 |
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B3 |
3 4 5 |
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A3 |
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Fraction |
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Collector |
NC |
NO |
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NC NO |
Sample loop |
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A2 |
A1 |
B2 |
B1 |
W2 |
W1 |
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W3 |
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6 |
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3 |
4 5 |
Outlet Valve (V4) |
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F3 |
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ÄKTApurifier
um 4
1
P-903
2 0.001 10ml/min 25MPa
UV-900
190-700nm UV-Vis 310mm 8µl 2mm 2µl
pH/C-900
pH
5
6
Frac-900
95 10 18 mm 175 12 mm 40 30 mm3
Frac-950
XY 4
A 120 18 & 30 mmB 240 12 mmC 96 4 30 mm 8D 45 30 mm
Frac-900 |
Frac-950 |
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V1
3 7
Load
Inject
Waste
PumpWash
Column |
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Column |
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Column |
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2 |
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2 |
7 |
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2 |
7 |
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Pump |
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Pump |
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Pump |
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Sample |
3 |
6 |
3 |
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syringe |
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6 |
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5 |
4 |
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5 |
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Waste |
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Waste |
Waste |
Waste |
Load |
Inject |
Waste |
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um 6
1
V1 2 6
10 µl 100 µl 1 ml
150 ml 10 ml 50 ml 150 ml 3
FR-902
0.2 MPa FR-904 0.4 MPa 2 FR-902
FR-902
FR-904
2 µm 2M NaOH18-1120-94 2
67 11.3
7
1.2 ÄKTApurifier
□&
□500 ml
□20 %
●
ÄKTA 2
↓
3
↓5
4
↓
4
↓
6
↓
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↓
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20 % |
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Evaluation |
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↓ |
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10 |
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ÄKTA |
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um 8
21
2
2.1 UNICORN
ÄKTA 2 1 ÄKTA 1PC CU-950 ÄKTA ÄKTACU-950 ÄKTA CU-950
1.CU-950 CU-950
2.ÄKTA
ON PC
OFF ON
3. ON
4. Windows UNICORN
5. Logon User name default
Password “default”
6. ÄKTA UNICORN Manager
9
6. ÄKTA System ControlRun data Instruments “Ready”CU-950 LED Power PC System 3
um 10
21
2.2 UNICORN
2.2.1 UNCORN
UNICORN 4 UNICORN Manager Method Editor System Control Evaluation6
UNICORN Manager |
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Copy, Rename, Delete, Move |
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Method Editor |
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System Control |
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Evaluation |
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Instant Run |
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Logon/Logoff |
UNICORN Logon/Logoff |
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Method Queue |
Method Queue Editor |
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Display running |
Method Queue |
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11
2.2.2
1
2.
3. Close
4.
5.
5-1 Manual
Manual ↓
5-2 Flowpath
Manual ↓ Flowpath...
5-3 Pump A Inlet
Manual ↓ Flowpath... → Pump A Inlet
5-4 A2
Manual ↓ Flowpath... → Pump A Inlet → A2
5-5 Execute
um 12
31
3
System Control Manual
3.1
W1 5 W24 W3 1 F3
PEEK 4 |
W3 |
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F2 |
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F3 |
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F3 |
3.2
20% 
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A |
B |
18-1132-75
13
3.3
ろ過では溶出時間が遅れ、イオン交換のようなグラジエント溶出ではグラジエ
右2 12
A1, A2, B1, B2 4
1 4 A1, B1
2 A
3
4 10 ml
5 A 2 4 A1
6 B 2
7 A2, B2
Manual ↓ Flowpath... → PumpAInlet → A2 → Execute A1 A2
Manual ↓ Flowpath... → PumpAInlet → B2 → Execute B1 B2
8 2 4 A2, B2
1A1
um 14
31
3.4
なお、このチェックは実験で使用するインレットのみで結構です。カラムは接続せず、インジェクションバルブとUV
1 System Control Run Data Curves
2 A A1 Manual ↓ Pump... → Flow → 5 ml/min → Execute
Pressure
3 B B1 Manual ↓ Pump... → Gradient → Target 100 %B, Length 0 min
→ Execute
Pressure
4 B B2 Manual ↓ Flowpath... → PumpBInlet → B2 → Execute
5 A A2 Manual ↓ Pump... → Gradient → Target 0 %B → Length 0 min
→ Execute
Manual ↓ Flowpath... → PumpAInlet → A2 → Execute
6 End
A1, A2, B1, B2 ±0.05MPa
3.5 PumpWash
1 System Control
2 5
Manual ↓ Pump... → PumpWash Purifier → ON, ON, ON, ON → Execute
PumpWash Continue Pause Continue
PumpWash → → waste → W1
15
3.6
1. UV G5
2.
Manual ↓ Pump... → Flow → 5ml/min → Execute
5 UV Cond Pressure
3. END
→ → Load → G5 →UV → → pH → →
W3
um 16
41
4
4.1
ÄKTAdesign valco 1/16 HiTrap FPLCM6
PEEK ÄKTApurifier 10 ÄKTApurifier UPC 10 0.5 mm PEEK ÄKTApurifier 100 ÄKTApurifier UPC 100 0.75 mm PEEK ÄKTApurifier 10 ÄKTApurifier UPC 10 0.25 mm PEEK
RESOURCE Tricorn 1/16 M6 1/16
18-3858-01
M6
18-1112-55
18-1112-55 |
18-1112-58 |
1/16 M6
18-1112-57 18-1112-55
HiTrap M6 HiLoad XK M6 HiPrep
FPLC HR M6
18-3858-01
18-1112-57 18-1112-55
18-1112-58
18-1112-55
HiTrap
18-1112-57 19-2143-01
HiLoad XK valco
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ÄKTAdesign |
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28-4010-81 |
11-0003-39 |
18-1112-55 |
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Union M6 female / 1/16” male |
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PEEK |
5 |
18-3858-01 |
Union 1/16” female / M6 male |
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PEEK |
6 |
18-1112-57 |
Union 1/16” male / M6 female |
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PEEK |
8 |
18-1112-58 |
Union 1/16” male / Union 1/16” male |
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PEEK |
2 |
18-1120-93 |
Fingertight connector, 1/16” male |
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PEEK |
10 |
18-1112-55 |
HiTrap / HiPrep 1/16” male connector for ÄKTAdesign |
PP |
8 |
28-4010-81 |
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Stop plug, 1/16” male |
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PEEK |
5 |
18-1112-52 |
Stop plug, 1/16” female |
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PEEK |
5 |
11-0004-64 |
Union, Luer female 1/16” male |
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PEEK |
2 |
18-1112-51 |
Union 1/16” female / 1/16” female |
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PEEK |
5 |
11-0003-39 |
Union M6 female / 1/16” female |
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PEEK |
2 |
18-1123-94 |
SRTC-2, M6 female (1.5 mm i.d.) |
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PEEK |
5 |
19-2143-01 |
Tubing (0.25 mm id, blue) |
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PEEK |
2 m |
18-1120-95 |
Tubing (0.50 mm id, orange) |
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PEEK |
2 m |
18-1113-68 |
Tubing (0.75 mm id, green) |
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PEEK |
2 m |
18-1112-53 |
Tubing (1.0 mm id, beige) |
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PEEK |
2 m |
18-1115-83 |
17
4.2
HiTrap HiPrep
UNICORN
Manual ↓ Alarms&Mon... → Alarm_Pressure → High Alarm → Execute
1
Manual ↓ Pump... → Flow → 0.5 ml/min → Execute
2 UV
3 END
4.3
20%
1 Pressure limit
Manual ↓ Alarms&Mon... → Alarm_Pressure → High Alarm → Execute
2
20%
Manual ↓ Pump... → Flow → 1/2 → Execute
1/4
3 3 UV Cond Pressure END
um 18
414
HiPrep ÄKTA
温では液の粘性が上昇するために送液圧力が上昇します。HiPrep 0.15MPa
る場合には十分ご注意ください。
ÄKTA
0.35 MPa HiPrep 0.15 MPa
FR-902 0.2 MPa
HiTrap
ÄKTA HiTrap
0.5 MPa HiTrap 0.3 MPa
FR-902 0.2 MPa
4.4
1.
Manual ↓ Pump... → Pumpwash Purifier → ON, OFF, ON, OFF → Execute
1 A1
2 A1/B1
BufferPrep A1 Buffer
A2 Acid/Base
B1 Water
B2 Salt
19
5
Column |
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1 |
Column |
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1 |
Column |
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1 |
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2 |
7 |
2 |
7 |
2 |
7 |
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Pump |
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Pump |
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Pump |
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Sample |
3 |
6 |
3 |
3 |
syringe |
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6 |
6 |
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4 |
5 |
4 |
4 |
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5 |
5 |
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Waste |
Waste |
Waste |
Waste |
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Load |
Inject |
Waste |
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5.1
1.2 6
2.3
3.4 5
が一体になったガラスシリンジをお使いいただくとサンプルのロスを減らせます。外径0.7mm 90
um 20
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