Page 1
HydrolEx H-506
Operation Manual
11593968 | B en
Page 2
Imprint
Product Identification:
Operation Manual (Original) HydrolEx H-506
11593968
Publication date: 01.2020
BÜCHI Labortechnik AG
Meierseggstrasse 40
Postfach
CH-9230 Flawil 1
E-Mail: quality@buchi.com
BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experience, especially with respect to structure, illustrations and technical details.
This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for competitive purposes, nor made available to third parties. The manufacture of any component with the aid of
this manual without prior written agreement is also prohibited.
Page 3
BÜCHI Labortechnik AG Table of contents
Table of contents
1 About this document........................................................................................................... 5
1.1 Warning notices in this document........................................................................................................ 5
1.2 Symbols............................................................................................................................................... 5
1.2.1 Warning symbols .....................................................................................................................5
1.2.2 Mark-ups and symbols.............................................................................................................5
1.3 Trademarks.......................................................................................................................................... 6
2 Safety....................................................................................................................................7
2.1 Proper use ........................................................................................................................................... 7
2.2 Use other than intended ...................................................................................................................... 7
2.3 Staff qualification ................................................................................................................................. 7
2.4 Location of warning signs on the product ............................................................................................ 8
2.5 Residual risks ...................................................................................................................................... 8
2.5.1 Hot surfaces.............................................................................................................................8
2.5.2 Faults during operation ............................................................................................................ 8
2.5.3 Glass breakage........................................................................................................................8
2.6 Personal protective equipment ............................................................................................................ 9
2.7 Modifications........................................................................................................................................ 9
3 Product description...........................................................................................................10
3.1 Description of function ....................................................................................................................... 10
3.1.1 Principle of hydrolysis ............................................................................................................ 10
3.2 Configuration ..................................................................................................................................... 11
3.2.1 Front view ..............................................................................................................................11
3.2.2 Rear view ...............................................................................................................................12
3.3 Type plate.......................................................................................................................................... 12
3.4 Scope of delivery ............................................................................................................................... 12
3.5 Technical data ................................................................................................................................... 12
3.5.1 HydrolEx H-506......................................................................................................................12
3.5.2 Ambient conditions.................................................................................................................13
3.5.3 Material .................................................................................................................................. 13
4 Transport and storage ......................................................................................................14
4.1 Transport ........................................................................................................................................... 14
4.2 Storage .............................................................................................................................................. 14
4.3 Lifting the instrument ......................................................................................................................... 14
5 Installation..........................................................................................................................15
5.1 Before installation .............................................................................................................................. 15
5.2 Installation site................................................................................................................................... 15
5.3 Establishing electrical connections.................................................................................................... 15
5.4 Securing against earthquakes ........................................................................................................... 16
5.5 Assembling the suction tube.............................................................................................................. 16
5.6 Assembling suction pump.................................................................................................................. 17
5.7 Assembling the lower rubber couplings............................................................................................. 17
6 Operation............................................................................................................................19
6.1 Assembling the sample aspiration tube............................................................................................. 19
6.2 Preparing the sample......................................................................................................................... 19
6.3 Performing a hydrolysis ..................................................................................................................... 21
6.4 Performing a filtration......................................................................................................................... 22
6.5 Ending a hydrolysis............................................................................................................................ 22
6.6 Shutting down the instrument ............................................................................................................ 22
Operation Manual HydrolEx H-506 iii
Page 4
Table of contents BÜCHI Labortechnik AG
7 Cleaning and servicing .....................................................................................................23
7.1 Regular maintenance work................................................................................................................ 23
8 Help with faults ..................................................................................................................25
8.1 Troubleshooting................................................................................................................................. 25
9 Taking out of service and disposal.................................................................................. 27
9.1 Disposal............................................................................................................................................. 27
9.2 Taking out of service.......................................................................................................................... 27
9.3 Returning the instrument ................................................................................................................... 27
10 Appendix ............................................................................................................................28
10.1 Spare parts and accessories ............................................................................................................. 28
10.1.1 Spare parts ............................................................................................................................28
10.1.2 Accessories............................................................................................................................29
10.1.3 Consumables .........................................................................................................................29
iv Operation Manual HydrolEx H-506
Page 5
BÜCHI Labortechnik AG About this document | 1
1 About this document
This operation manual is applicable for all variants of the instrument.
Read this operation manual before operating the instrument and follow the instructions to ensure safe and trouble-free operation.
Keep this operation manual for later use and pass it on to any subsequent user or
owner.
BÜCHI Labortechnik AG accepts no liability for damage, faults and malfunctions resulting from not following this operation manual.
If you have any questions after reading this operation manual:
u Contact BÜCHI Labortechnik AG Customer Service.
https://www.buchi.com/contact
1.1 Warning notices in this document
Warning notices warn you of dangers that can occur when handling the device. There
are four danger levels, each identifiable by the signal word used.
Signal word Meaning
DANGER Indicates a danger with a high level of risk which could result in
death or serious injury if not prevented.
WARNING Indicates a danger with a medium level of risk which could result in
CAUTION Indicates a danger with a low level of risk which could result in mi-
NOTICE Indicates a danger that could result in damage to property.
1.2 Symbols
The following symbols may be displayed in this instruction manual or on the device:
1.2.1 Warning symbols
Symbol Meaning
death or serious injury if not prevented.
nor or medium-severity injury if not prevented.
Hot surface
General warning
Breakable items
1.2.2 Mark-ups and symbols
NOTE
This symbol draws attention to useful and important information.
R This character draws attention to a requirement that must be met before the in-
structions below are carried out.
u This character indicates an instruction that must be carried out by the user.
Operation Manual HydrolEx H-506 5/30
Page 6
1 | About this document BÜCHI Labortechnik AG
ð This character indicates the result of a correctly carried out instruction.
Mark-up Explanation
Win dow Software Windows are marked-up like this.
Tab Tabs are marked-up like this.
Dia log Dialogs are marked-up like this.
[Button] Buttons are marked-up like this.
[Field names] Field names are marked-up like this.
[Menu / Menu item] Menus or menu items are marked-up like this.
Status Status is marked-up like this.
Signal Signals are marked-up like this.
1.3 Trademarks
Product names and registered or unregistered trademarks that are used in this document are used only for identification and remain the property of the owner in each
case.
6/30 Operation Manual HydrolEx H-506
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BÜCHI Labortechnik AG Safety | 2
2 Safety
2.1 Proper use
The instrument is designed and built for laboratories. It serves for the hydrolysis of
samples for fat determination with the use of diluted hydrochloric acid.
2.2 Use other than intended
Use of any kind other than that described in Chapter2.1 "Proper use", page7 and
any application that does not comply with the technical specifications constitutes use
other than that intended.
In particular, the following applications are not permissible:
Use of the instrument in rooms which require ex-protected instruments.
Use of samples, which can explode or inflame (example: explosives, oxidizing,
etc.) due to shock, friction, heat or spark formation.
Use in overpressure situations.
Use without ventilation or fume hood.
Use with flammable substances.
2.3 Staff qualification
Unqualified persons are unable to identify risks and are therefore exposed to greater
dangers.
The device may only be operated by suitably qualified laboratory staff.
These operating instructions are aimed at the following target groups:
Users
Users are persons that meet the following criteria:
They have been instructed in the use of the device.
They are familiar with the contents of these operating instructions and the applica-
ble safety regulations and apply them.
They are able on the basis of their training or professional experience to assess
the risks associated with the use of the device.
Operator
The operator (generally the laboratory manager) is responsible for the following aspects:
The device must be correctly installed, commissioned, operated and serviced.
Only suitably qualified staff may be assigned the task of performing the operations
described in these operating instructions.
The staff must comply with the local applicable requirements and regulations for
safe and hazard-conscious working practices.
Safety-related incidents that occur while using the device should be reported to the
manufacturer (quality@buchi.com).
BUCHI service technicians
Service technicians authorized by BUCHI have attended special training courses and
are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair
measures.
Operation Manual HydrolEx H-506 7/30
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2 | Safety BÜCHI Labortechnik AG
2.4 Location of warning signs on the product
Fig.1: Location of warning signs
Hot surface
2.5 Residual risks
The device has been developed and manufactured using the latest technological advances. Nevertheless, risks to persons, property or the environment can arise if the
device is used incorrectly.
Appropriate warnings in this manual serve to alert the user to these residual dangers.
2.5.1 Hot surfaces
The surfaces of the device can become very hot. If touched they can cause skin
burns.
u Do not touch hot surfaces or else wear suitable protective gloves.
2.5.2 Faults during operation
If a device is damaged, sharp edges, moving parts or exposed electrical wires can
cause injuries.
u Regularly check device for visible damage.
u If faults occur, switch off the device immediately, unplug the power cord and in-
form the operator.
u Do not continue to use devices that are damaged.
2.5.3 Glass breakage
Broken glass can cause severe cuts.
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BÜCHI Labortechnik AG Safety | 2
Minor damage to the ground joints impairs the sealing effect and may therefore diminish suction capacity.
Handle the glass components carefully and do not drop them.
Always place the glassware in a suitable holder when they are not in use.
Always visually inspect glass components for damage every time they are to be
used.
Do not continue to use glass components that are damaged.
Always wear protective gloves when disposing of broken glass.
2.6 Personal protective equipment
Depending on the application, hazards due to heat and/or corrosive chemicals may
arise.
u Always wear appropriate personal protective equipment such as safety goggles,
protective clothing and gloves.
u Make sure that the personal protective equipment meets the requirements of the
safety data sheets for all chemicals used.
2.7 Modifications
Unauthorized modifications may impair safety and lead to accidents.
u Use only genuine BUCHI accessories, spare parts and consumables.
u Technical modifications to the device or accessories should only be carried out
with the prior written approval of BÜCHI Labortechnik AG and only by authorized
BUCHI technicians.
BUCHI accepts no liability whatsoever for damage arising as a result of unauthorized
modifications.
Operation Manual HydrolEx H-506 9/30
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3 | Product description BÜCHI Labortechnik AG
3 Product description
3.1 Description of function
The hydrolysis frees fatty substances which are mechanically surrounded by different
constituents (carbohydrates and protein in undamaged cells or starch membranes).
This is also true for colloid-disperse fractions (protein) which, as a result of surface
forces, coat the fat drops (milk, cream, cheese) and for certain fat fractions which are
bound, chemically or adsorptively to other components (phosphatide-protein complexes in yeast, eggs, etc.).
For fat determination including acid hydrolysis such as the method according to
Weibull-Stoldt in food and animal feed, the product is to be hydrolyzed to make the fat
accessible for extraction. Thus a reproducible fat determination is possible.
3.1.1 Principle of hydrolysis
1. The sample is boiled in hydrochloric acid to break down proteins and high-molecular carbohydrates into acid-soluble constituents.
2. The sample is filtered over a layer of Celite® and sand in a glass sample tube and
dried. The free fat in the hydrolysate adsorbs on the Celite® in the glass sample
tube. The underlying sand layer prevents the Celite® from reaching the frit of the
glass sample tube.
3. The fat can be extracted with an appropriate solvent.
10/30 Operation Manual HydrolEx H-506
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BÜCHI Labortechnik AG Product description | 3
9
8
7
5
6
10
11
12
4
32
1
3.2 Configuration
3.2.1 Front view
Fig.2: Front view
1 Sample aspiration tube 2 Lift device
3 Rinsing funnel 4 Rack
5 Hydrolysis vessel 6 Upper insulation plate
7 Lever 8 Power regulator
9 On/Off master switch 10 Suction tube
11 Stopcock 12 Glass sample tube with frit
Operation Manual HydrolEx H-506 11/30
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3 | Product description BÜCHI Labortechnik AG
1
2
3
4
3.2.2 Rear view
Fig.3: Rear view
1 Power supply connection 2 Fuse
3 Fuse 4 Type plate
3.3 Type plate
The type plate identifies the instrument. The type plate is located at the rear of the instrument.
3.4 Scope of delivery
NOTE
The scope of delivery depends of the configuration of the purchase order.
Accessories are delivered as per the purchase order, order confirmation, and delivery note.
3.5 Technical data
3.5.1 HydrolEx H-506
Specification HydrolEx H-506 for 115 V HydrolEx H-506 for 230 V
Dimensions (W x D x H) 312 x 614 x 470 mm 312 x 614 x 470 mm
Minimum clearance (W xD)200 mm 200 mm
Minimum clearance (H) 300 mm 300 mm
Weight
(without glassware)
12/30 Operation Manual HydrolEx H-506
13 kg 13 kg
Page 13
BÜCHI Labortechnik AG Product description | 3
Specification HydrolEx H-506 for 115 V HydrolEx H-506 for 230 V
Weight
16.5 kg 16.5 kg
(with glassware)
Connection voltage 110 - 120 ± 10 % VAC 220 - 240 ± 10 % VAC
Fuse 10 A 10 A
Frequency 50 / 60 Hz 50 / 60 Hz
Overvoltage category II II
Protection class 1 1
Pollution degree 2 2
IP Code - -
Power consumption 1200 W 1200 W
Temperature range heater 70 - 495 °C 70 - 495 °C
Total Heating power 1200 W 1200 W
Number of hydrolysis positions
Approvals CE / CSA CE / CSA
3.5.2 Ambient conditions
For indoor use only.
Max. altitude above sea level 2000 m
Ambient temperature 5‒40 °C
Maximum relative humidity 80% for temperatures up to 31 °C
Storage temperature max. 45 °C
3.5.3 Material
Component Materials of construction
Housing Stainless Steel
Insulation plate heating PROMATECT MST
Rinsing funnel Polypropylene
Hydrolysis vessel Borosilikat 3.3
6 6
decreasing linearly to 50 % relative humidity at 40 °C
Sample aspiration tube Borosilikat 3.3
Rubber coupling FKM
Glass sample tube Borosilikat 3.3
Suction tube Borosilikat 3.3
Stopcock PTFE
Vacuum hose Natural rubber
Operation Manual HydrolEx H-506 13/30
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4 | Transport and storage BÜCHI Labortechnik AG
4 Transport and storage
4.1 Transport
NOTICE
Risk of breakage due to incorrect transportation
Make sure that the instrument is fully dismantled.
Pack every instrument components properly to prevent breakage. Use the original
packaging whenever possible.
Avoid sharp movements during transit.
u After transporting, check the instrument and all glass components for damage.
u Damage that has occurred in transit should be reported to the carrier.
u Keep packaging for future transportation.
4.2 Storage
u Make sure that the ambient conditions are complied with (see Chapter3.5 "Tech-
nical data", page12).
u Wherever possible, store the device in its original packaging.
u After storage, check the device, all glass components, seals and tubing for dam-
age and replace if necessary.
4.3 Lifting the instrument
NOTICE
Dragging the instrument can damage the feet of the instrument.
u Lift the instrument when positioning or re-locating.
u Lift the instrument at the points indi-
cated.
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BÜCHI Labortechnik AG Installation | 5
5 Installation
5.1 Before installation
NOTICE
Instrument damage due to switching it on too early.
Switching on the instrument too early after transportation can cause damage.
u Climatize the instrument after transportation.
5.2 Installation site
The installation site must meet the following requirements:
Firm, level surface
Take into account the maximum product dimensions and weight. See Chapter3.5
"Technical data", page12
Fume hood
Make sure that cables / tubes can be routed safely
Own mains outlet socket
Own water tap (only when using a water jet pump)
NOTE
Make sure that the power supply can be disconnected at any time in an emergency.
5.3 Establishing electrical connections
NOTE
Observe the regulatory provisions when connecting the instrument to power supply.
4 Use external main switches (e.g., emergency off) in accordance with the stan-
dards IEC 60947-1 and IEC 60947-3.
4 Use additional electrical safety features (e.g., residual-current circuit breakers) to
comply with local laws and regulations.
The power supply must fulfill the following conditions:
1. Provide the mains voltage and frequency specified on the type plate of the instrument.
2. Be designed for the load imposed by the instruments connected.
3. Be equipped with suitable fuses and electrical safety features.
4. Be equipped with a proper grounding.
Operation Manual HydrolEx H-506 15/30
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5 | Installation BÜCHI Labortechnik AG
NOTICE
Risk of property damage and diminished performance due to use of unsuitable power cables.
The power supply cables supplied with the product by BUCHI precisely match the requirements of the device. If other power cables that do not meet those requirements
are used, the device may be damaged and/or its performance diminished.
u Use only the power supply cables supplied with the product or ordered separately
from BUCHI.
u If using any other power supply cables, make sure that they match the specifica-
tions on the type plate.
u Make sure that all connected devices are grounded.
u Make sure that the power plug is freely accessible at all times.
u Insert the power cable into the connection labeled Power IN on the back of the in-
strument.
u Insert the power plug into the power socket.
5.4 Securing against earthquakes
NOTE
4 Use two eye screws M4 x 10.
4 Screw-in depth 10 mm.
u Attach two eye screws to the instrument.
u Attach the instrument to a fixed point using a
strong cord or a wire.
5.5 Assembling the suction tube
NOTE
Malfunction because of incorrect assembled suction tube.
Make sure:
4 That the stopcocks are freely accessible.
4 That the openings are facing upright.
u Switch the On/Off master switch to Off.
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BÜCHI Labortechnik AG Installation | 5
u Put the suction tube on the support.
u Fasten the suction tube on the support with
the screws.
ð The suction tube is assembled
5.6 Assembling suction pump
There are two possibilities of vacuum supply:
Vacuum pump see Chapter10.1.2 "Accessories", page29
Waterjet pumpt see Chapter10.1.2 "Accessories", page29
Precondition:
R The suction tube is assembled. See Chap-
ter5.5 "Assembling the suction tube", page16
u Moisten the vacuum tube with water.
u Install the vacuum tube onto the connection at
the suction tube.
u Connect the other side of the vacuum tube to
the vacuum supply.
5.7 Assembling the lower rubber couplings
NOTICE
Broken glass
Hold the suction tube on a point not indicated can cause glass breakage.
u Hold the suction tube at the point indicated.
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5 | Installation BÜCHI Labortechnik AG
Precondition:
R The suction tube is assembled. See Chap-
ter5.5 "Assembling the suction tube", page16
u Moisten the rubber coupling with water.
u Slip over the rubber coupling on the suction
tube.
u Make sure that the rubber coupling is in the
correct position.
ð The rubber coupling is assembled.
u Assemble all rubber couplings in the same
way.
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BÜCHI Labortechnik AG Operation | 6
6 Operation
6.1 Assembling the sample aspiration tube
NOTICE
Broken glass
Hold the sample aspiration tube on a point not indicated can cause glass breakage.
u Hold the sample aspiration tube at the point indicated.
u Moisten the upper rubber coupling with water.
u Slip over the top rubber coupling on the sam-
ple aspiration tube.
u Make sure that the upper rubber coupling is in
the correct position.
ð The sample aspiration tube is assembled.
u Assemble all upper rubber couplings in the
same way.
6.2 Preparing the sample
NOTE
The maximum sample weight is 10 g.
Before performing a hydrolysis prepare the sample.
Precondition:
R All commissioning operations have been completed. See Chapter5 "Installation",
page15
R There are no samples in the instrument.
u Put the lever at the instrument in the lower position.
Operation Manual HydrolEx H-506 19/30
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6 | Operation BÜCHI Labortechnik AG
u Put 2 g Celite® 545 into the hydrolysis vessel.
u Weigh-in sample.
u Add 50 mL 4 M HCl and mix the sample with the
Celite® and the HCl.
u Rinse the walls of the hydrolysis vessel with 50 mL, 4 M
HCl.
NOTE
Use quartz sand with a grain size of 0.3 - 0.9 mm.
4 In case the grain size is too small it will clog the frit of the glass sample tube.
4 In case the grain size is too large the Celite® will pass through the frit of the sam-
ple tube during filtration.
u Apply approx. 20 g quartz sand equally in the glass
sample tube.
u Add a layer of 2 g Celite® 545 in the glass sample tube.
20/30 Operation Manual HydrolEx H-506
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BÜCHI Labortechnik AG Operation | 6
3
7
6
5
2
1
4
u Insert the glass sample tube in the rubber coupling at
the instrument.
ð The glass sample tube is prepared.
6.3 Performing a hydrolysis
NOTE
To reduce processing time preheat the instrument.
Fig.4: Instrument assembled for hydrolysis
1 Sample aspiration tube 2 Rinsing funnel
3 Hydrolysis vessel 4 Suction tube
5 Upper rubber coupling 6 Glass sample tube with frit
7 Lower rubber coupling
Precondition:
R All installations are done. See Chapter5 "Installation", page15.
R The samples are prepared.
R The sample aspiration tube is assembled. See Chapter6.1 "Assembling the sam-
ple aspiration tube", page19.
u Switch the On/Off master switch in position On.
u Turn the power regulator to position Preheat.
u Wait 10 minutes.
u Close the stopcocks for the unused positions.
u Put the hydrolysis vessel in the rack at the instrument.
u Put the rinsing funnel onto the hydrolysis vessels.
u Lower the hydrolysis vessels by lifting the lever.
u Install the sample aspiration tube.
u Turn the power regulator to position 2.5.
u As soon as all samples start boiling switch on the suction pump.
Operation Manual HydrolEx H-506 21/30
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6 | Operation BÜCHI Labortechnik AG
6.4 Performing a filtration
NOTE
Only use distilled water with a temperature between 40 and 50 °C.
Precondition:
R All samples are hydrolyzed according the requirements.
u Turn the power regulator to position Off.
u Add water in each position.
u Lift the hydrolysis vessels by lowering the lever.
ð The samples from the hydrolysis vessels will be moved by suction.
u Rinse the hydrolysis vessels.
u Wait until the content of the hydrolysis vessel is completely transferred.
u Repeat the rinsing step of the hydrolysis vessel three times.
u Remove the sample aspiration tube.
u Remove the glass sample tube from the rubber coupling.
u Perform a pH-test.
The following results are possible:
Neutral
Acidic
Neutral
Precondition:
R The result of the pH-test is neutral.
u The hydrolysis is completed.
Acidic
Precondition:
R The result of the pH-test is acidic.
u Put the sample tube back to the rubber couplings at the instrument.
u Install the sample aspiration tube.
u Rinse the hydrolysis vessels.
u Perform a pH-test.
6.5 Ending a hydrolysis
u Dry the sample for further processing.
6.6 Shutting down the instrument
u Switch the On/Off master switch to Off.
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BÜCHI Labortechnik AG Cleaning and servicing | 7
7 Cleaning and servicing
NOTE
Users may only carry out the servicing and cleaning operations described in this section.
Any servicing and repair work which involves opening up the casing may only be carried out by BUCHI service technicians.
u Use only genuine BUCHI consumables and spare parts in order to ensure correct
operation of the device and preserve the warranty.
7.1 Regular maintenance work
CAUTION
Hot surfaces.
Skin burns from hot surfaces.
u Let the instrument cool down sufficiently before carrying out any maintenance
work.
Component Action Frequency
Glass parts u Clean the glass parts with commer-
Daily
cially available cleaning agents.
u If heavily soiled, use ethanol or a mild
detergent.
u WARNING!Do not clean the glass
sample tube with frit in an ultrasonic bath.
u Dry the glass parts fully.
u Check each part visually for cracks,
scratches and for any parts or sections that might have splintered off.
u Replace the damaged glass part.
Rubber couplings u Rinse the rubber couplings with wa-
Daily
ter.
Suction tube u Rinse the stopcocks.
u Make sure the stopcocks move eas-
Weekly
ily.
Housing u Wipe down the casing with a damp
Weekly
cloth.
u If heavily soiled, use ethanol or a mild
detergent.
Warning symbols u Check that the warning symbols on
Weekly
the instrument are legible.
u If they are dirty, clean them.
u If illegible, replace them.
Heating chamber u Remove dust and foreign objects us-
Monthly
ing compressed air or a vacuum
cleaner.
Operation Manual HydrolEx H-506 23/30
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7 | Cleaning and servicing BÜCHI Labortechnik AG
Component Action Frequency
Control panel u Wipe down the display with a damp
Monthly
cloth.
Rubber couplings u Replace the rubber couplings. Yearly
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BÜCHI Labortechnik AG Help with faults | 8
8 Help with faults
8.1 Troubleshooting
Problem Action
Insufficient boiling u Make sure that the power supply is
connected.
u Make sure that both heating coils are
working properly (glowing).
u Make sure that the instrument has
been preheated for 10 minutes.
u Make sure that the power regulator is
set between 2 - 3.
Excessive foam formation during
hydrolysis
Hydrolysate cannot be transferred
through the aspiration tube
u Make sure that the power regulator is
set between 2 - 3.
u Add some drops of 4 M HCl.
u Reduce sample weight.
u Make sure that the aspiration tube, all
rubber couplings and all hoses are
connected properly.
u Make sure that the glass parts are not
cracked or broken.
u Make sure that the hose is not por-
ous.
u Make sure that the water jet pump or
vacuum pump is switched on.
u Close the stopcocks on unused posi-
tions.
u Close the stopcocks on finished posi-
tions to increase the suction capacity
on difficult position.
u Reduce the amount of sample.
Celite® 545 is washed out during rinsing u Make sure to use quartz sand with a
grain size of 0.3 - 0.9 mm.
u Make sure the right amount of quartz
sand and Celite® 545 is chosen.
Glass sample tube with frit blocked u Rinse the frit thoroughly to remove
any remaining quartz sand and
Celite® 545 prior to cleaning in a
dishwasher. Please refer to the glass
sample tube cleaning guide.
u Do not sonicate the glass sample
tube.
u Make sure that the quartz sand with
grain size of 0.3-0.9 mm is used.
u Replace glass sample tube.
Operation Manual HydrolEx H-506 25/30
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8 | Help with faults BÜCHI Labortechnik AG
Problem Action
Breakage hydrolysis vessel Precondition:
R WARNING!Wear gloves during
carrying out the following instructions.
u Let the temperature of the instrument
decrease until it is the same as the
ambient temperature.
u Remove glass debris.
u Wipe the heating chamber with a
damp cloth.
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Page 27
BÜCHI Labortechnik AG Taking out of service and disposal | 9
9 Taking out of service and disposal
9.1 Disposal
The operator is responsible for proper disposal of the instrument.
u When disposing of equipment observe the local regulations and statutory require-
ments regarding waste disposal.
u When disposing, observe the disposal regulations of the materials used. Materials
used see Chapter3.5 "Technical data", page12
9.2 Taking out of service
u Switch off the instrument and disconnect it from the mains power supply.
u Remove all cables from the device.
9.3 Returning the instrument
Before returning the instrument, contact the BÜCHI Labortechnik AG Service Department.
https://www.buchi.com/contact
Operation Manual HydrolEx H-506 27/30
Page 28
10 | Appendix BÜCHI Labortechnik AG
10 Appendix
10.1 Spare parts and accessories
Use only genuine BUCHI consumables and spare parts in order to ensure correct,
safe and reliable operation of the system.
NOTE
Any modifications of spare parts or assemblies are only allowed with the prior written
permission of BUCHI.
10.1.1 Spare parts
Order no. Image
Insulation plate 11065169
Suction tube, cpl. 11064546
Set of hydrolysis vessels, 2 pcs. 11064547
Set of aspiration tubes, 2 pcs. 11064548
Set of aspiration tubes, long, 2 pcs. 11069475
Glass sample tubes with frit, 6 pcs. 11067497
Glass sample tubes with frit, long, 6 pcs.
The glass sample tubes with 150 mm length fit perfectly into the Universal glass extraction chamber.
Set of rinsing funnels, 2pcs. 11067582
11067815
Vacuum hose, 2 m 040459
28/30 Operation Manual HydrolEx H-506
Page 29
BÜCHI Labortechnik AG Appendix | 10
Order no. Image
Set of upper couplings, FKM, 3 pcs. 11068611
Set of lower couplings, FKM, 3 pcs. 11068612
10.1.2 Accessories
Order no. Image
Water jet pump. Plastic
Used when tap water is used to generate vacuum.
002913
Suction set with pump
Vacuum pump V-100, bottle, tubing
11068473
Holder for glass sample tubes, PTFE 11067220
Holder for glass sample tubes, stainless steel 11067219
Weighing support for hydrolysis vessels 11067040
Hydrolysis vessel carrier 11067492
Adapter clips for B-411 sample tubes, 4 pcs.
These adapters allow the use of glass sample tubes for
B-411/ B-811 on combination with the HydrolEx H-506.
In addition the rubber couplings of the E-416 / B-411
are needed for the use in the H-506. EPDM (037381)
or Viton (044491)
11069239
10.1.3 Consumables
Order no.
Quartz sand 0.3 - 0.9 mm, 2.5 kg 037689
Celite® 545, 1 kg 11068920
Quartz sand (25 kg) 034925
Operation Manual HydrolEx H-506 29/30
Page 30
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