Xylem OC100 User Manual

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Operating Manual

System OxiTop® Control

OxiTop® OC100

Controller

OxiTop®-C

Measuring Heads

OC Model

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Safety Instructions

This instrument is built and checked according to IEC 1010, safety rules for electronic measuring instruments and left the factory secure from a safety engineering aspect.

The smooth functioning and operational safety of the equipment can only be guaranteed by following the general safety precautions applicable and the special safety instructions given in this operating manual.

– The trouble-free function and operational safety of the instrument can only be guaranteed by following the climate conditions specified in the chapter "Technical data" in this operating manual.

– If the system is transported from a cold environment to a warm environment, its function can be impaired as a result of condensation forming. In this case, the temperature of the equipment must be allowed to adapt to room temperature before putting it into operation again.

– Adjustment of the equipment and maintenance or repair work must only be performed by personnel authorized by WTW.

– If safe operation is no longer possible, the equipment must be taken out of service and secured against inadvertent operation by labeling with warning signs.

– The safety of the user can be affected by the instrument if, for example,

• the instrument is visibly damaged,

• the instrument no longer operates as prescribed,

• the instrument has been stored under adverse conditions for a lengthy period of time,

• the instrument was exposed to adverse transport conditions.

– Basically, if you are in any doubt, please return the instrument for repair or maintenance to the manufacturer of the equipment, "Wissenschaftlich-Technische-Werkstätten GmbH".

Please read these safety instructions carefully before installing the instrument!

Contents

Safety Instructions .................................................................................................... 3

Contents.....................................................................................................................4

Introduction ...............................................................................................................6

The measuring principle ........................................................................................7

The OxiTop

Operating modes ...................................................................................................8

Litterature.........................................................................................................8

Operating Elements ..................................................................................................9

Controller keybord .................................................................................................9

General Operating Principles.................................................................................10

Switching On ...........................................................................................................11

Switching on the controller...................................................................................11

Switching on the measuring heads .....................................................................11

Operating Mode: Routine BOD...............................................................................12

Sample preparation .............................................................................................12

Start the measurement ........................................................................................12

Call up all data ....................................................................................................15

Sample management...........................................................................................17

Show sample ......................................................................................................19

Erase data of finished samples ...........................................................................20

Show measuring head list....................................................................................21

Call up data .........................................................................................................22

Call up data - Stop ...............................................................................................24

Evaluation............................................................................................................25

Curve display of samples that are too cold ....................................................26

Measured values outside the measurement ranges ......................................26

Printing.................................................................................................................27

Operating Mode: Standard BOD ............................................................................29

Sample preparation .............................................................................................29

Start the measurement ........................................................................................29

Call up all data ....................................................................................................32

Sample management...........................................................................................35

Show sample ......................................................................................................36

Erase data of finished samples ...........................................................................37

Show measuring head list....................................................................................38

Call up data .........................................................................................................39

Call up data - Stop ...............................................................................................42

Evaluation............................................................................................................44

Sample statistics..................................................................................................45

Excluding a curve ................................................................................................46

Cursor interrogation .............................................................................................48

Curves display for cold samples ....................................................................49

Measured values outside the measuring range .............................................50

Print .....................................................................................................................51

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Control measuring system ...............................................................7

Contents

GLP/Tools ................................................................................................................53

GLP/Tools main menu .........................................................................................53

Show free measuring heads ................................................................................54

Show settings ......................................................................................................55

Settings................................................................................................................56

Operating mode .............................................................................................56

Measuring time...............................................................................................57

Date/Time.......................................................................................................57

GLP ................................................................................................................58

GLP - calibrating interval (”Calinterval”) ...................................................59

Memory ..........................................................................................................60

AutoTemp.......................................................................................................61

Switch-off interval...........................................................................................63

Language .......................................................................................................64

Check...................................................................................................................65

Show measuring heads..................................................................................65

Measuring head info.......................................................................................66

Controller info.................................................................................................67

Cal test ...........................................................................................................68

Pneumatic test measuring head.....................................................................71

Maintenance ........................................................................................................72

Erase finished samples..................................................................................72

Reset/release measuring head ......................................................................74

RS232 Interface .......................................................................................................76

Cleaning ...................................................................................................................77

Cleaning the sample bottles ................................................................................77

Cleaning the controller and measuring heads .....................................................77

Power Supply...........................................................................................................78

OxiTop®-OC100 controller ...................................................................................78

OxiTop

Disposing of the batteries ....................................................................................83

What to do if ...? ......................................................................................................84

Display messages................................................................................................84

Requirements / Problems ....................................................................................90

Accessories and Spare Parts.................................................................................95

Technical Data .........................................................................................................96

Note: This operating manual refers to software release 1.xx. The right to implement minor changes is reserved.

-C measuring head.................................................................................82

Introduction

The OxiTop® Control system provides a further development of the mercury-free, measured value storing OxiTop a multitude of new technological features that simplify the whole process of the BOD determination for the user. Furthermore, the instrument is almost unlimited with respect to its sample capacity and use. It can also be expanded flexibly to fulfill its tasks. In addition to its application in the water economy, the OxiTop ”respirometric” measuring principle can also be used in other areas such as

• Evaluation of biological degradability, i.e. testing aerobic degradation (e.g. test according to OECD 301F)

• Evaluation of respiration and toxicity in earth, sludge, waste and sediment (e.g. extraction of earth contaminated according to recovery concepts)

• Evaluation of the respiration rate of cell cultures

• Microbiological growth and stress examinations

BOD measurement system. The instrument provides

Control with its

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Introduction

In addition, in conjunction with the enhanced OxiTop accessories, the OxiTop

-C measuring heads can also be used to

Controller OC110 and relevant

• Measure anaerobic degradation processes (e.g. biogas evaluation)

The measuring principle

The sample bottles are filled according to the data visible in the display (BOD measuring range with assigned water sample amount). The microorganisms draw oxygen to degrade organic substances from the amount of air remaining in the closed system. The carbon dioxide formed by this process is absorbed. Due to the reduction in the amount of oxygen, the pressure in the bottle sinks. This change is detected and stored by the measuring head. After the data transfer to the controller, it is used to determine the BOD value. Further information is given in the WTW BOD handbook.

The OxiTop® Control measuring system

The 6 or 12 position OxiTop and consist of the OC100 OxiTop inductive stirring system and various accessories. The measured value determination and storage of the measurement data is performed in the measuring head. This data is transmitted via ”infrared to the controller” without the need for wires and then calculated to the BOD value. The user can start his individual measuring program and printout the calculated data via the TD100 IR printer or further process it via a PC program using the ”Routine BOD” and ”Standard BOD” operating modes described in detail in this operating manual. The user interface appears in one of 5 selectable languages. An overview of the entire sample management together with the status and function response is shown on the display. With the aid of the display function, sample identification is no longer required. Via the OxiTop to 144 measuring positions.

An overview of the essential advantages

• DIN procedure • Modular extensions

• Mercury-free • Data storage

• Precise • Comfortable handling

• Automatic sample management

• Automatic measurement report

Control systems are configured for BOD determination

controller, the OxiTop®-C measuring heads, the

-C IS 6/12 expansion sets, the system can be extended up

• Wireless data transfer, (even through glass or transparent plastic)

General Operating Principles

Operating modes

The OC100 controller has 2 standard operating modes that differ from one another as described below:

”Routine BOD”

This mode provides the user-friendly ”quick introduction”. The samples are managed as single samples. The setting functions are logically restricted.

”Standard BOD”

The main difference to the ”Routine BOD” mode is in the parallel sample process for up to 12 measuring heads per whole sample with mean value formation and statistical evaluation. The setting is made via the function ”GLP/Tools”. The extended setting functions allow a multitude of further options (see chapter ”GLP/Tools”). The samples plotted in the ”Standard BOD” mode are not displayed in the ”Routine BOD” mode.

A table of the settings in the delivery state is given in the chapter ”GLP/Tools”.

Literature

Further information on this subject can be obtained at no cost from WTW:

- BOD handbook

- Application reports

- Special printouts

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Switching On

Controller keyboard

Sample management: List of samples, reading the data of individual

measuring heads or samples, displays of measuring heads or samples

Printing of measurement data and settings

Switching on/off

GLP / Tools: Display free measuring heads, display or change settings,

perform checks or maintenance

Evaluation: Graphic and numerical display of measuring data

Communication with measuring heads: Start measuring, call up data

Select

Confirmation of entries

General Operating Principles

Representation of keys and displays:

Example: Pressing the ”Communication” button causes the mode to change to the ”Communication with the measuring heads” mode of operation:

Required action: press button.

The instrument displays:

Description of what the action caused and possible further request:

Starting communication with the measuring heads. Select a menu item using

Function keys:

These function keys are used to start or change a function. Confirmed data and settings are preserved.

Display:

- In selection menus, the selected function or line appear in a lighter font on a

dark background

- :

Measuring head:

Each measuring head that receives a command from the controller, indicates this by a short flashing signal.

Signal tone: This symbol means: A signal tone sounds.

Notes:

Instructions and additional information appear in italics.

Symbol for an OxiTop®-C measuring head

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Switching On

Switching on the controller

The current date and time appear (important for the allocation of sample numbers). If the date/time is incorrect, undertake corrections in ”GLP/Tools”.

The instrument is in the sample management (Routine mode, in the delivery state).

Switching on the measuring heads

The measuring heads are immediately ready for operation. The controller switches the measuring heads independently on and off during communication.

To avoid malfunctions: In case of using two or more controllers please pay attention to the following:

Absolutely avoid starting measuring heads simultaneously between controllers is less than 3 meters when doing this.

if the distance

Operating Mode: Routine BOD

Operating mode: Routine BOD

Sample preparation

See WTW application reports (contained within the scope of delivery of the accessories supplied).

Screw the OxiTop and tightly close them.

Important note: Never use joint grease or other lubricant for your OxiTop

heads. Some of these products contain solvents that can cause severe damage to the plastic housing. The sealing of the BOD bottles is also perfectly adequate without grease. However, you should always wipe off heavy contamination and particles on the sealing surfaces of the rubber sleeves and OxiTop liability for damage due to the use of joint grease.

Start the measurement



-C measuring heads onto the sample bottles



-C. WTW accepts no

Entry into the mode “Communication with the measuring heads“.

Preselected: Start

Use to select the measurement range. The filling volume required is given in the right-hand column.

The controller stores the setting (memory function: the last selected measurement range is set).

(measuring head).



-C measuring

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Operating Mode: Routine BOD

Confirm the selected measurement range for the sample. The automatically allocated sample number is given in the header line (YY/MM/DD and sequential number).

Additional information: Type of measurement, run time, measurement range, final date, Id number. “Temp“ display => the AutoTemp function (see the chapter “GLP/Tools”) is set as a default in the routine mode.

Change the Id number of the additional identification of the sample (e.g. extraction location) as follows:

- Use

- Press and

- Use (setting range 001 ... 255).

- Use

to move the cursor to the Id number,

to set the Id number required

to confirm this.

Confirm the start of the measurement. Contact selection:

The controller repeatedly sends the start information in the scanner mode until successful feedback is received from the OxiTop®-C measuring head.

Operating Mode: Routine BOD

Displays “Started“. From this point in time, the sample exists within the

If no sample starts (e.g. because the controller was not held to - or not

held close enough to - a measuring head):

sample management. Subsequently, the controller returns automatically to the input menu.

The sample start was stopped.

Use

If an attempt is made to start an already started sample:

“Continue controller to the measuring head (see above).

Use “Stop start“ if you want to return to the entry menu.

to confirm

start“ and hold the

In the delivery state, the controller automatically makes space when “Start erases the oldest finished sample (if a finished sample is available). You can change this setting in “manual erase” (see the chapter “GLP/Tools Settings”).

” is selected if the measured value memory is full. To do this, it

Display shows “Measuring head already used!”

Use

” and hold the controller to the

measuring head (see above).

Use “Stop start“ if you want to return to the entry menu.

to confirm “New

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Operating Mode: Routine BOD

Call up all data

This function is used to call up the data of all measuring heads. For the call up of the data of individual measuring heads: see the chapter on sample management.

Entry menu “Communication with the measuring heads“.

Point the controller at the measuring heads:

Use to select “Call up all data“.

40 cm to 100 cm

Queries the data of all active measuring heads in the scanner mode.

The controller stores the data obtained and updates the list of the sample management. Duration of a pass: approx. 3 seconds for 12 measuring heads in a stirring system.

Operating Mode: Routine BOD

If all the measuring heads do not respond in the 1st pass of the data query, the controller searches for the missing measuring heads for approximately a further 7 seconds in the scanner mode. Without having to press another key, you can continue the data queries at other locations (e.g. other stirring platforms or other thermal cabinets). Approximately 7 seconds after the last call up was performed, the scanner mode breaks off automatically and the following display appears:

Point the controller at the measuring heads:

“Continue transfer“ (preselected): A new pass is made only for measuring heads that have not yet responded.

Stop: The instrument returns to the entry menu. For information on searching for missing measuring heads, see the chapter “What to do if...“

40 cm to 100 cm

The call up of further measuring heads takes place in the scanner mode.

Message that the data of all measuring heads has been called up. The instrument subsequently returns to the input menu.

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Operating Mode: Routine BOD

Immediately after the data call up of the complete measurement data records of a finished measuring head, this measuring head is set to the “free“ status. The measuring head is now available for a new measurement. The sample allocated in the sample management is “finished“ (see the chapter “Sample management”).

Sample management

Entry into the sample management. A list of samples appears in the display (if samples are available):

BOD type and run-duration

Sample number: Date (YY/MM/DD) and consecutive number

Data of samples that have been started in the standard BOD operating mode, are not listed in the routine BOD operating mode.

Reporting order:

- At the upper end of the list: finished samples (if available)

- Under this: running samples

Sorting of the samples: according to date and sequential number 01 ... 99 from the oldest to the newest sample.

Temporal process of the sample:

Status bar partly filled: The sample is not yet ready.

Status bar filled: The ready and complete data set of the sample can be called up from the measuring head.

Status bar filled plus hook: The sample is ready. The complete data set is given in the controller for evaluation.

Operating Mode: Routine BOD

Select a sample.

Depending on the sample selected, one of the two following menus appears. The header line contains the:

- sample number

- Id number (e.g. I001)

- BOD type and run time of the selected sample.

Finished sample

Current sample

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Operating Mode: Routine BOD

Show sample

This function is used to find the measuring heads. A sample labeling is not necessary.

Starting point: sample management .

Point the controller at the measuring heads:

Use to select a sample.

Use to select “Show sample“.

40 cm to 100 cm

The controller transmits the call up of the selected sample. The allocated measuring head flashes for approx. 5 seconds.

After the message, the controller automatically returns to the

previous menu.

Operating Mode: Routine BOD

Erase data of finished samples

This function erases the data of finished samples from the sample management of the controller. You can only erase a sample if it is finished; in non-finished samples, the menu item “Erase sample“ does not appear.

Starting point: sample management .

Use to select a finished sample.

Use to select the “Erase sample“ submenu.

Safety query with possibility to return.

The sample has been erased.

After the message, the controller automatically returns to the starting

menu, “Sample management“.

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Operating Mode: Routine BOD

Show measuring head list

This function is used to allocate a measuring head to the relevant sample.

Point the controller at the measuring heads:

Use to select “ list“.

“List“, i.e. a measuring head is displayed together with the serial number of the measuring head.

The controller transmits the call up of the selected sample. The allocated measuring head flashes for approx. 5 seconds.

40 cm to 100 cm

Operating Mode: Routine BOD

Call up data

This function is used to call up the data of individual measuring heads. To call up the data of all measuring heads, see the chapter “Call up all data“.

Use to select a sample whose

- run time is not yet completed (bar not yet full)

- data after complete measurement that has not yet been called up (bar full, no tick)

Jumps to the submenu of the selected sample. Preselected: “Call up data“

Point the controller at the measuring heads:

40 cm to 100 cm

Call up the measuring head allocated to the sample in the scanner mode: The controller

- stores the data obtained

- updates the lists of the sample management

The controller confirms the call up performed.

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Operating Mode: Routine BOD

If the measuring head does not respond, the following display appears:

The controller then returns automatically to the submenu.

The controller continues to search for the measuring head in the scanner mode. Without pressing any further keys, the data call up can be continued at other locations (e.g. other stirring platforms or other thermal cabinets). After approx. 7 seconds, the scanner mode automatically breaks off and the following display appears:

Use to restart the call up of the data (see above).

Return to the main menu, “Sample management“. Samples that were called up and completed appear with a tick next to them.

Call up data - Stop

Operating Mode: Routine BOD

If a measuring head is missing or defective and the controller cannot transfer the data as a result, the menu item “Stop“ is provided.

After confirmation, the display message shown here appears.

Then:

“Show

“Missing

“:

As in “Show sample“ but used selectively for missing measuring heads (identification option if only the sender of the measuring head is defective. Otherwise identify missing measuring heads using “Show all“ (see the chapter “GLP/Tools”).

Using this you can remove the data of a missing or defective measuring head from the sample management of the controller.

Erase: Removes missing samples from the data stock.

Return: Jumps back to the previous menu.

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Operating Mode: Routine BOD

Evaluation

Change to cursor prompt:

Starting point: sample management.

Use to select a sample.

Enters into the evaluation: Display of the selected sample as a curve together with measured value data.

Pressing causes the cursor (crosswire) to run through the points of the curve. The allocated run time and the corresponding measured value are each displayed.

Pressing display of the curve together with the measured value data.

changes between the cursor prompt and

Operating Mode: Routine BOD

Curve display of samples that are too cold

Display of a curve

In using samples that are too cold, the maximum AutoTemp time is not sufficient to reach the temperature adaption of the sample: By warming the sample, excess pressure can result.

Display: Negative values of the curve are truncated and the curve emerges from the time axis but not from the origin.

Measured values outside the measurement ranges

In the following cases, the evaluation displayed is not the measured value but “undef.“ (undefined):

- The measurement curve exceeds the measurement range at some point of its path.

- The measurement curve undercuts the measurement range at its end point.

Sample displays:

The measured value exceeds the measurement range (overflow).

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Operating Mode: Routine BOD

The measured value undercuts the measurement range (underflow).

Printing

Point the controller at the printer:

Switch on the TD 100 IR printer.

The controller outputs the report data of the selected sample on the IR interface. Printout of:

- sample information (start date and time, final date, type, measurement range, identification number)

- curve + BOD final value

- report printouts: - current settings

- start information

- controller info

The measured value temporarily leaves the measurement range.

10 cm to 100 cm

info

Operating Mode: Routine BOD

From the sample management: From the evaluation:

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Operating Mode: Standard BOD

Operating mode: Standard BOD

Sample preparation

See WTW application reports (contained within the scope of delivery of the accessories supplied).

Screw the OxiTop and close them tightly.

Important note: Never use joint grease or other lubricant for your OxiTop

Start the measurement

To change to the operating mode BOD Standard refer to chapter GLP/Tools Settings - Operating mode.



-C measuring heads onto the sample bottles



-C measuring



-C. WTW accepts no

“Communication with the measuring heads” mode.

Preselected: “Start sample”.

Use to select the measurement range. The filling volume required is given in the right hand column.

The controller stores the setting (memory function: the last selected measurement range is set).

Operating Mode: Standard BOD

Additional information: Type of measurement, run time, measurement range, final date, Id number. “Temp“ display => the AutoTemp function (see the chapter “GLP/Tools”) is switched on.

Change the Id number for the additional identification of the sample (e.g. extraction location) as follows:

- Use

- Press and

- Use (setting range 001 ... 255).

- Use

to move the cursor to the Id number,

to set the Id number required

to confirm this.

Confirm the selected measurement range for the sample. The automatically assigned sample number (YY/MM/DD and sequential number) is given in the header line.

Use possible (see the chapter “Standard BOD - Print”).

to print out the entire sample information

Confirm the start of measurement. Contact selection:

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Operating Mode: Standard BOD

The controller repeatedly sends the start information in the scanner

mode until successful feedback is received from the OxiTop®-C measuring head. After the successful start message from the measuring head:

Displays “!Started!”. From this point in time, the sample exists within the sample management.

Subsequently, the request to start the next measuring head is made automatically.

Start the next measuring head

2).

( Contact selection:

The number of the measuring head is automatically incremented.

The controller continues to work with the stirring selection, i.e. you

can now start a sequence of measuring heads (parallel sample start) without having to press any further keys by holding the controller to the next measuring head to be started.

When all the measuring heads provided for this sample have been started:

Use The controller returns to the input menu.

to selecct “Stop start”:

If no measuring head is started (e.g. because the controller was not held to - or not close enough to - a measuring head):

Operating Mode: Standard BOD

The start was stopped.

Use

If an attempt is made to start a measuring head that was already

started:

“Continue controller to a measuring head (see above).

Select “Stop start” to return to entry level menu.

Displays “Measuring head already used!”

Use

” and hold the controller to a free measuring head (see above).

to confirm

start” and hold the

to confirm “New

Select “Stop start” to return to entry level menu.

In the delivery state, the controller automatically makes space when “Start it deletes the oldest finished sample (if a finished sample is available). You can change this setting in “manual erase” (see the chapter “GLP/Tools - Settings”).

” is selected if the measured value memory is full. To do this,

Call up all data

This function is used to call up the data of all measuring heads. To call up the data of individual measuring heads: see the chapter “ Sample management”.

Entry menu “Communication with the measuring heads“.

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Operating Mode: Standard BOD

Use to select “Call up all data”.

Point the controller at the measuring heads:

40 cm to 100 cm

Queries the data of all active measuring heads in the scanner mode.

The controller stores the data obtained and updates the list of the sample management. Duration of a pass: approx. 3 seconds for 12 measuring heads in a stirring system.

If the measuring heads do not all respond in the 1st pass of the data query, the controller searches for the missing measuring heads in the scanner mode for approximately a further 7 seconds. Without having to press another key, you can continue the data queries at other locations (e.g. other stirring platforms or other thermal cabinets). Approximately 7 seconds after the last call up was performed, the scanner mode breaks off automatically and the following display appears:

“Continue transfer“ (preselected): A new pass is made only for measuring heads that have not yet responded.

Stop: The instrument returns to the entry menu. For information on searching for missing measuring heads, see

Operating Mode: Standard BOD

Point the controller at the measuring heads:

the chapter “What to do if...“

40 cm to 100 cm

The call up of further measuring heads is performed in the scanner mode.

Message that all the data of all the measuring heads has been called up. The instrument then returns to the input menu.

Immediately after the data transfer of the complete measurement data records of a finished measuring head, this measuring head is given the “free” status. The measuring head can be used for a new measurement. The relevant sample in the sample management is marked as finished (see the chapter “Sample management”).

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Operating Mode: Standard BOD

Sample management

Entry into the sample management. A list of samples appears in the display (if samples are available):

Data of samples that have been started in the standard BOD operating mode is not listed in the routine BOD operating mode.

Reporting order:

- At the upper end of the list: finished samples (if available)

- Under this: current samples

Sorting of the samples: according to date and sequential number 01 ... 99 from the oldest to the newest sample.

Select a sample.

Operating Mode: Standard BOD

According to the sample selected, one of the two following menus appears. The header line contains the:

- sample number

- Id number (e.g. I001)

- BOD type and run time of the selected sample.

Finished sample

Current sample

Show sample

This function is used to find the measuring heads or samples. Sample labeling is not necessary.

Starting point: sample management .

Use to select a sample.

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Operating Mode: Standard BOD

Point the controller at the measuring heads:

40 cm to 100 cm

The controller transmits the call up of the selected sample. The allocated measuring head flashes for approx. 5 seconds.

After the message, the controller automatically returns to the

previous menu.

Erase data of finished samples

Starting point: sample management .

Use to select a finished sample.

Use to select the “Erase sample“ submenu.

Operating Mode: Standard BOD

Safety prompt with possibility to return.

The sample has been erased.

After the message, the controller automatically returns to the starting

menu, “Sample management“.

Show measuring head list

This function is used to allocate individual measuring heads to the relevant sample in the sample management and to find individual measuring heads.

Use to select “Measuring head list”.

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Operating Mode: Standard BOD

Point the controller at the measuring heads:

List of measuring heads of the selected sample is displayed together with the serial numbers of the measuring heads.

40 cm to 100 cm

Use confirm. The controller again transmits the call up of the selected sample. The measuring head flashes for approx. 5 seconds.

Use

to select the measuring head and use to

to return to the sample management.

Call up data

This function is used to call up the data of individual samples. To call up the data of all samples, see the chapter “Call up all data“.

Use to select a sample whose

- run time is not yet completed (bar not yet full)

- data after complete measurement that has not yet been called up (bar full, no tick)

Operating Mode: Standard BOD

Point the controller at the measuring heads:

Jumps to submenu of the selected sample. Preselected: “Call up data“

40 cm to 100 cm

Sequential call up of the measuring heads allocated to the sample (in this example: 7) in the scanner mode: The controller

- stores the data obtained

- updates the list of the sample management

Duration of a pass: approx. 3 seconds for 12 measuring heads in a stirring system.

The controller confirms the call up performed.

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Operating Mode: Standard BOD

The controller then returns automatically to the submenu.

If the measuring heads do not all respond, the following display appears:

Renewed start of the data call up.

The controller continues to search for missing measuring heads in the scanner mode. Without pressing any further keys, the data call up can be continued at other locations (e.g. other stirring platforms or other thermal cabinets). Approximately 7 seconds after the last request was performed, the scanner mode automatically breaks off and the following display appears:

Use to restart the the data transfer (see above).

Immediately after the successful data transfer of a sample, the allocated measuring heads are given the “free” status. The measuring heads can be used for a new measurement.

Return to the main menu, “Sample management“. Transferred and completed samples appear with a tick next to them. (The complete measurement data records of all finished measuring heads of the sample have been procured.)

Call up data - Stop

Operating Mode: Standard BOD

If a measuring head is missing or defective and the controller cannot completely call up the sample as a result, the menu item “Stop“ is provided to stop the data transfer.

After confirmation, the display message shown here appears.

The sample is still running and individual measuring heads of the sample are not attainable.

Case 1

Three displays are then possible:

Case 2

The run time of the sample has ended and the finished, complete data of the attainable measuring heads has been transferred.

42 BA31114e5 07/2006 BA31114e5 07/2006 43

Operating Mode: Standard BOD

then

Case 3:

then

“Show

“Missing

”:

Erase: Removes missing measuring heads from the data stock. The completed sample is given the “finished” status.

Back: Jumps back to the previous menu.

Abort using a function key: The stopped sample is not declared as “finished”.

No measuring head of a sample is attainable.

Erase: Removes a sample from the data stock.

Back: Jumps back to the previous menu.

As in “Show sample“ but selectively for missing measuring heads (Identification option if only the sender of the measuring head is defective. Otherwise, see the chapter “What to do if...?”)

Only appears when the run time of the sample has expired. It is used to set the sample to the “finished” status (ticked) if all the other measuring heads are already “finished”: Erases the missing measuring heads from the sample management.

Evaluation

Operating Mode: Standard BOD

Starting point: Sample management.

Use sample.

to select the

Printout of the results with curves.

Evaluation of the whole sample: Display of all the curves together with mean value data. Check the display for outliers.

Printout of the results with curves (according to printing format see the chapter “Print“)

Example: Whole sample with outliers.

Scroll through the parallel samples: Display of individual curves (cyclical pass) with data of the BOD final value.

Printout of a single result with a single curve.

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Operating Mode: Standard BOD

Sample statistics

When jumping to the pull-down menu from “Show all curves“, the menu item “Exclude curve“ disappears.

Results of the finished sample: Data of the current sample:

Starting point: display of a single curve.

Changes to sample statistics.

The menu selection shown here only appears if n (the number of measuring heads) is at least 2. If n = 1, see the chapter “Routine BOD - Evaluation”.

- mean value

- SD: standard deviation (from n = 3)

- n: number of measuring heads

- end date

- current mean value

- n: number of measuring heads

Printout of the results with curves

Printout of the data (current mean value, n, sample information) with curve paths to date

Operating Mode: Standard BOD

Excluding a curve

This function is used to temporarily exclude a single curve (e.g. an outlier) from the evaluation and averaging of a whole sample. The curve is only excluded temporarily! The data stock of the sample management does not change. The excluded curve is present again when the call is repeated.

The function “List of measuring heads“ (see the chapter “Sample management”) is used to find leaky or defective single samples.

Display of the single curves:

Select curve.

Change to “Exclude curve“.

“Exclude curve“ is preselected.

“Return“: Returns to the previous menu.

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Operating Mode: Standard BOD

Message “Curve excluded“.

Updated display (curve excluded, mean value newly calculated).

Printout of the updated results with curves (without the excluded curve).

Operating Mode: Standard BOD

Cursor interrogation

Starting point: Display of a single curve with measured value data.

Use to change to “Cursor query“.

Or from the display of all the single curves:

Use to run through the curve values.

Use current display.

Use to run through the curve mean values.

to print out the

Use to print out the current display.

Return to the previous menu using

Return to the display of all the single curves using

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Operating Mode: Standard BOD

Curves display for cold samples

Display of a single curve.

When using samples that are too cold, the maximum AutoTemp time is not sufficient to reach the temperature balance of the sample: Warming the sample can cause excess pressure.

Display: Negative values of the curve will be truncated and the curve arises from the time axis but not the origin.

Display of all curves.

Operating Mode: Standard BOD

Measured values outside the measuring range

In the following cases, “undef.“ (undefined) is displayed instead of the measured value or mean value during evaluation:

- A measured curve exceeding the measuring range at any point of its path.

- A measured curve undercutting the measuring range at its end point.

After the exclusion of the defective curve (see chapter “Exclude curve“), the controller displays the mean value again.

Sample displays:

A measured value exceeding the measuring range (Overflow).

A measured value undercuts the measuring range (Underflow).

A measured value temporarily leaves the measuring range.

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Operating Mode: Standard BOD

Point the controller at the printer:

Switch on the TD100 IR printer.

10 cm to 100 cm

The controller outputs the report data of the selected sample to

the IR interface. Printout of

- sample statistics (mean value, SD, n)

- sample information (type, running time, measuring range, start date and start time, end date, identification no.)

- curve + BOD final value

- with GLP On: additional information on the system status and list of the series nos. of the measuring heads allocated to the sample

- If AutoTemp is switched on: note “AutoTemp on“

- Report printouts: - current settings

- start information

- controller info

From the sample management: From the evaluation (display of

info

all the curves):

Operating Mode: Standard BOD

Print from the evaluation:

If the controller is located

- in the display of all the curves with cursor display or

- in the display of single curves with or without cursor query, the printout shows a copy of the relevant display.

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GLP / Tools

GLP/Tools main menu

Main menu:

Use

Submenus of the GLP/Configuration:

Show free

Show settings The current settings are displayed here.

Setup point Preset

Operating mode Routine BOD

Routine BOD: Standard BOD:

BOD type and measuring time

Date Current Current

Time Current Current

GLP: ON or OFF Off (fixed setting) On

Calibrating interval --- 12 months

Erase memory auto

AutoTemp: ON or OFF

Switch-off interval 5 minutes (fixed setting) 5 minutes

Language German German

This function is used to identify free measuring heads without requiring sample labeling. Free measuring heads can be used to start new samples.

(The table shows the settings in the delivery state):

BOD5 (5 days) BOD5 (5 days)

(automatic erase)

On (fixed setting) On

to select a submenu

Use to change to the submenu

auto (automatic erase)

GLP / Tools

Settings

Check Show

Here you can undertake or change the following settings:

Operating mode Measuring time Date/time * GLP; calibrating interval Memory * AutoTemp * Switch-off interval Language

(finished/all)

info (with report printout)

Controller info (with report printout) Cal test Pneumatic test

Maintenance Erase finished samples (in the controller - sample management)

Reset/release

* only in the standard BOD operating mode

Show free

Main menu, “GLP/Tools“. The “Show free is preselected.

“ menu

Press .

Point the controller at the measuring heads:

40 cm to 100 cm

All free measuring heads flash for approx. 5 seconds.

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GLP / Tools

Show settings

The following example illustrates the presettings in the Standard BOD operating mode.

Main menu, “GLP/Tools“.

Use to select the “Show settings“ menu.

“Show settings“ menu: A list containing the current settings is displayed.

Use to scroll through the list where the scrolling stops at the beginning and end of the list

In the “Show settings“ submenu: Print out the whole list of current settings (as in the display).

Settings

Operating mode

GLP / Tools

Main menu, “GLP/Tools“.

Use to select the “Settings“ menu.

“Settings“ submenu:

Use to select “Operating mode“.

Use to acknowledge

it and to select between the operating modes, Routine BOD and Standard BOD.

Confirm using

. Return to the “Settings“ main menu:

, then .

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GLP / Tools

Measuring time

Set the type and measuring time of the BOD measurement here.

“Settings“ submenu:

Use to select the “Measuring time“ submenu.

Use to set the days (1 to 99) or hours (0.5 to 23).

Confirm using

Display: preset measuring time. Default on delivery: 5 days.

Date/Time

Set the date and time in the controller here (important for sample number allocation).

“Settings“ submenu:

Use to select the “Date/Time“ submenu.

GLP / Tools

Set each of the number blocks underlaid in black using

Confirm and continue with

The measuring time of samples already started is not affected by a change of date and time.

Set the day, month, year, hours, minutes, seconds consecutively.

GLP

The GLP (Good Laboratory Practice) set of rules presents particular requirements concerning test resource monitoring for the measuring systems implemented (quality assurance). The following points must be fulfilled to comply with the requirements of GLP,:

1. Documentation of the calibration intervals.

2. Reporting of the calibrations: Identification no. of the measuring head/controller Date and signature of the operator.

3. Determination of the calibration frequency and instructions for the performance: The frequency depends on the operating conditions and the data of the test plan.

The OxiTop This mode inhibits measuring if the calibrating interval is exceeded. As a result of these restrictions, the GLP mode effects a standardization of the calibrating and measuring conditions that are indispensable for working from the aspect of quality assurance.

Control system supports these standards using an add-on GLP mode:

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GLP / Tools

Switch the GLP function on or off (only in the standard BOD operating mode):

“Settings“ submenu:

Use to select the “GLP“ submenu.

“GLP“ submenu. “GLP“ is preselected.

Press

then use to select either GLP “On“ or “Off“

Confirm using

GLP - calibrating interval (“Calinterval”)

Set the time period (1 to 36 months) here. When it expires, the instrument registers that the next test resource monitoring of the measuring heads is due. After the calibrating interval expires, measuring is blocked until the calibration is performed or the GLP setting is set to “Off“.

If the end of the calibrating interval set up lies within the measuring time of a measurement to be started, this measurement cannot be started.

“GLP“ submenu.

Use to select the subitem, “Calinterval“.

GLP / Tools

Press , then set the time period for the calibrating interval (1 - 36

months) using Default: 12 months.

Confirm by

Memory

Here, you can set whether the controller should automatically erase the oldest finished samples if the memory is full to create space for new measurement data (setting “auto“). With the “manual“ setting, the message “Lack of memory! Erase finished samples!“ is displayed if the memory is full (See chapter “What to do if ...?).

“Settings“ menu:

Use to select the “Memory“ submenu.

Erase memory is preset to “automatic“. To change it:

Press

use to select between automatic and manual and

use to acknowledge it.

Return to the “Settings“ menu:

then

and

60 BA31114e5 07/2006 BA31114e5 07/2006 61

GLP / Tools

AutoTemp

The AutoTemp function controls the automatic start of the measurement after checking the temperature adaption. The pretemperature regulation to the precise incubator temperature is recommended but not essential. Recommendation: e.g. regulate the temperature of the sample for BOD5 measurements from 15 °C up to 20 °C. You can tightly close the sample bottle with the measuring head immediately and start the measurement. The AutoTemp function then automatically triggers the start of the actual measuring after checking the temperature adaption. The measuring time of the AutoTemp phase (adaption phase plus the test phase) is added to the sample measuring time selected in the settings.

Pretemperature regulation of the sample

With the AutoTemp function switched on and, adhering to the recommendation according to the table, the error quota that results from the temperature adaption of the sample to the incubator temperature, T selected measurement range final value.

Measuring time of the

measurement

Recommended sample temperature at the start of the

1 day T

2 days T

3 days T

4 days T

5 ... 99 days T

BOD5 15°C ... 20°C

, is smaller than 1% of the

Incubator

measurement

-0.5K ... T

Incubator

-1K ... T

Incubator

-2K ... T

Incubator

-3K ... T

Incubator

-5K ... T

Incubator

AutoTemp function in detail The AutoTemp function is made up of the adaption phase and the test phase.

Adaption phase

The phase without evaluation of the pressure process. The duration of the adaption phase is defined for the various measuring times according to the table. The adaption of the microbiology to the characteristics of the sample is made in this phase and small temperature deviations, too high and too low temperatures of the sample, can be compensated.

Test phase The phase in which the further pressure process direction in the sample bottle is checked. The test phase is defined for various measuring times according to the table. In this phase, the continuing temperature deviation can be compensated if the temperature of the sample is too low.

GLP / Tools

Sequence of the test phase:

With a further drop in pressure (consumption) or constant pressure after the adaption phase, the pressure value at the end of the adaption phase is the starting point of the measurement. On a further increase of pressure following the adaption phase (the sample is still too cold), the turning point of the pressure process at which the pressure increase changes into a pressure drop, is the starting point of the measurement. If no starting point is found (according to the procedure given in points 1 and 2) after the termination of the AutoTemp phase (time limit exceeded), the last measuring point of the AutoTemp phase forms the starting point. This means that the BOD curve in the graphical evaluation does not emerge from the coordinate origin at the zero time point. A sample that was too cold was started.

BOD measuring time

0.5 to 23 hours Since the measurement times are very short, the system

1, 2, 3, 4, 5 days 14, 28, 42, 56, 70 minutes Maximum of 28, 56, 84, 112,

6 to 99 days in a 1 day pattern

Switch the AutoTemp function on or off (only in the standard BOD operating mode):

Adaption phase duration Test phase duration

always suppresses the AutoTemp phase here even if the AutoTemp function is switched on in the settings.

70 minutes Maximum of 140 minutes

140 minutes

“Settings“ menu:

Use to select “AutoTemp“ submenu.

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GLP / Tools

AutoTemp is preset to “On“. To change:

Press

and use to select between AutoTemp “On“ and “Off“ and

use

Return to the “Settings“ menu:

then

to confirm it.

Switch-off interval

The time interval after the last time a key is pressed can be set here. After this time interval expires, the controller switches itself off to save energy. (Settings of 5 to 15 minutes are possible, only in the standard BOD operating mode.)

“Settings“ menu:

Use to select the “Switch-off interv.“ submenu.

The switch-off interval is preset to 5 minutes. To change it:

Press

use to set the no. of minutes required and

again to confirm it.

Return to the “Settings“ menu:

then

and

GLP / Tools

Language

Select the language here in which the displays of the OxiTop® Controller appear. The controller has the following 5 languages stored in it (default German): German English - French - Italian - Spanish.

“Settings” menu:

Use to select the “Language“ submenu.

The default language is “German“. To change this:

Press

use to set the language required and

again to confirm it. From now on the displays appear in the selected language.

Return to the “Settings“ menu:

then

and

“Settings“ menu. Return to the main menu, “GLP/Tools“:

then

using

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Check

Show

GLP / Tools

Main menu, “GLP/Tools“.

Use to select the “Check“ menu.

“Check“ menu. “Show

“ preselected.

Point the controller at the measuring heads:

All the measuring heads that are addressed flash for 5 seconds.

“Show “ submenu.

Use to select between “Show free “Show all

“ and

“.

40 cm to 100 cm

info

GLP / Tools

“Check“ menu.

Use to select “ info“.

The measuring head flashes and a display appears on the controller giving the following information:

- serial number of the measuring head,

- battery status (OK/LOBAT/EMPTY!!),

- the next calibration date (only in the measuring mode, Standard BOD and GLP “On“

- status of the measuring head (free/used/defective).

If the measuring head is “used“,

use to scroll through the display of further information:

- start date

- final date

- sample number

- type and

- range for the measurement

Return to the “Check“ menu

using

Repeat the procedure for each measuring head.

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GLP / Tools

If the measuring head does not respond, the following display appears after approximately 7 seconds:

Controller info

The query was stopped. You can select between

- continue query (see above) and

- Stop (return to the “Check“ menu

“Check“ menu.

Use to select “Controller info“.

The display shows the following information:

- number of measuring heads for which there is still memory capacity

- status of the supply batteries

- status of the data backup batteries (OK/LOBAT).

Use specification of the

- software version

- Ser.no. (can differ from the example shown here)

Return to the “Check“ menu

using

to scroll to the

GLP / Tools

Cal test

The Cal test is used to test the sealing of the system measuring head - rubber sleeve - sample bottle and the operability of the systems OxiTop

In the standard BOD operating mode with the “GLP ON” setting, the controller indicates when the next Cal test is due after each set up calibration interval (see the chapter “GLP/Tools - GLP”),. To perform the test, you need the WTW test resource, OxiTop 209 333. Sample preparation: see operating manual, OxiTop

PM test resource.

Control.

PM, order number

“Check“ menu.

Use to select “Cal test”.

For information on the further handling of the sample up to the “finished” status: See the chapter “Start”.

The Cal sample appears together with the other samples in the sample management. The BOD type is “CAL“:

The instrument automatically allocates the sample number (in the header line). The filling volume (164 ml) and the type together with the run time of 5 days are preset.

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GLP / Tools

Evaluation of the Cal test

In the standard BOD operating mode and GLP “On“:

Starting point: sample management.

Use to select the test sample.

Enter the evaluation. Display of the selected sample as a curve with measurement value data.

Compare measurement values with batch test value (according to operating manual,

OxiTop

Now you can set a new calibration date for the measuring head with the controller.

- “Show “ (to find the relevant measuring head): Function and messages as described in the chapter “Sample management“.

- “Set cal date“ (appears only if the Cal test is finished and the controller is in the standard BOD operating mode, GLP is switched on and the test is finished).

- see below.

- “Stop“: Return to the curve display.

Use to select “Set cal date“.

PM).

GLP / Tools

The controller sets a new test date in the measuring head. The controller calculates the new test date from the current date + the check interval set up (see the chapter “GLP/Tools - Settings GLP - Check interval”).

The controller displays the setting of the calibration date.

If the date was not set successfully (e.g. because the controller was not held to - or not held close enough to - the measuring head):

Repeat the procedure using

, then continue as described above.

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GLP / Tools

Pneumatic test

The pneumatic test tests the measurement precision of the measuring head. It says nothing about the long-term impermeability of the system.

To perform the pneumatic test, you require the testing agent, OxiTop order number 209 334).

The user interface of the controller guides you through the test:

“Check“ menu.

Use menu item “Pneumatic test

PT (WTW

to select the

“.

Set the plunger of the siringe on the OxiTop 5 scale parts. Tightly screw the measuring head to be tested onto the OxiTop PT tesing agent.

PT testing agent to

(Exceeding the time causes a return to the “Check“ menu“).

GLP / Tools

Maintenance

(Exceeding the time causes a return to the “Check“ menu).

The controller display shows the result of the pneumatic test.

Use to return to the “Check“ menu.

Main menu, “GLP/Tools“.

Use to select “Maintenance“ menu.

Erase finished samples

Here you can erase the data of finished samples that is already evaluated or no longer required in order to free memory in the controller.

Menu item “Erase samples“ is preset.

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GLP / Tools

A submenu appears with the selection:

- From sample no. (preselected)

- All

- Back (from the “Maintenance” submenu)

The controller displays the list of finished samples. The oldest finished sample is marked.

With , you can mark further samples.

With marking again.

The controller asks again if you really want to erase the marked samples from the memory.

, you can remove the

After confirmation, the display message shown here appears for 2 seconds and then the controller returns to the menu “Erase finished samples“.

GLP / Tools

In the selection of erase “All“ samples, the following display appears:

The controller asks again if you really want to erase the samples from the memory.

Further: See above.

If no finished samples are available in the memory, the following

display appears:

Reset/release

This function can be used to release measuring heads again that were unintentionally started. Caution: After carrying out the “Reset/release“, the data of the measuring head is erased!

Use to select the Reset/release

menu item.

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GLP / Tools

The serial number of the measuring head and the sample number appear on the display.

Press release the measuring head.

(If you do not want to release the measuring head, select and confirm it with “Return“.)

if you want to

Display message: The release/ reset has been performed.

Repeat the process for each of the measuring heads to be released.

Display message when the last measuring head of a sample has

been released:

Use Reset/release

to return to the

menu.

RS232 interface

RS232 Interface

The RS232 interface is used for communication with the computer (PC). The RS232 interface is provided solely for the implementation of the WTW software, “Achat OC“ (WTW software “ACHAT OC“ and RS232 interface cable AK 540/B available from 1998).

Brief information about the software “Achat OC” (requires Microsoft Windows)

• Downloads the sample management of the controller to the PC

• Comfortably displays the sample management together with additional information

on the screen

• Enables the selection of samples in the PC and transfers the measurement data of the selected samples from the controller to the PC

• Creates files from the measurement data for further processing with tabular calculation programs

RS232 interface

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Cleaning

Cleaning the sample bottles

See the WTW application report.

Cleaning the controller and measuring heads

• Do not use any solvent (such as alcohol or acetone)!

Use a soft, damp cloth and dilute soapy solution for cleaning.

•

Power Supply

The OxiTop®-OC100 and OxiTop®-C instruments are battery-powered. To ensure reliable operation, both instruments have a 2-stage battery status monitor 1st level = warning level : Batteries LoBat ! 2nd level = error level : Batteries empty !

OxiTop

Economy circuit (automatic switch off)

The instrument switches off automatically following the last key actuation after expiry of the set switch-off interval.

Switch-off interval:

• Routine BOD operating mode: preset to a fixed period of 5 min.

• Standard BOD operating mode: delivery state of 5 min.,

settings of 5...15 min. possible

Supply batteries

Batteries: 3 pcs, alkaline (alkaline manganese), size: Mignon, AA, AM3, LR6 These batteries ensure the energy supply of the OxiTop Run time: > 100 h (approx. 1000 start-ups in normal use)

Supply batteries status signals are given on switching on the instrument by display messages and a signal tone or can be called up under “GLP/Tools - Check - Controller info”.

Display message

Supply

Battery/ies LoBat !

Please change !

Supply

Battery/ies empty!

Please change !

-OC100 controller

Note / Meaning

The warning appears for approx. 3 seconds. The instrument then continues to run normally. The instrument can still be safely operated within the specifications. When the message first appears, there is still a running reserve available. Please obtain new batteries and replace the old ones !

The message appears for approx. 3 seconds. The instrument then switches itself off. The instrument can no longer be used. The supply batteries must be replaced by new ones.

OC100.

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Power Supply

Changing supply batteries:

• Switch off the OxiTop

• Loosen the 4 screws underneath the housing using a Phillips screwdriver (see

figure

• Place the controller on the lower case.

• Remove the upper case and put it down to the right next to the lower case with

the display downwards (see figure

• Remove the supply battery holder from the fixing in the lower case and turn it around.

• Remove the empty supply batteries.

• Turn the supply battery holder around again and place it in the fixing in the lower

case.

• Set the upper case on top of the lower case, turn the controller and tighten the housing screws using the screwdriver (see figure

• Switch on the controller. The battery change was successful if no error message appears concerning the supply batteries.

• It is recommended to use only brand name batteries of the type specified as “Alkaline“. Using other types of battery can affect reliable functioning.

• The supply batteries have no influence on data integrity.

• Insert new supply batteries - 3 pieces, alkaline (alkaline manganese),

size: Mignon, AA, AM3, LR6). Ensure that they are the right way round! (The poles are marked in the supply battery holder) Always replace the complete set of batteries.

-OC100 controller.

Power Supply

Data backup battery

1 x lithium battery, CR2430, e.g. WTW type Batt/OxiTop order no. 209 012. This battery ensures that your measurement data and the instrument settings in the OxiTop years

Data backup battery - status messages

are given on switching on the instrument by display messages and a signal tone or can be called up under “GLP/Tools - Check - Controller info”.

Display message

Data protection battery/ies LoBat!

Please change !

- OC100 controller are saved. Run time: typically 4

Note / Meaning

The warning appears for approx. 3 seconds. The instrument then continues to run normally. The instrument can still be operated within the specifications. When the message first appears, there is still a running reserve available. Please obtain new batteries and replace the old ones.



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Power Supply

Changing the data backup battery:

• Evaluate and save all the measurement data. Options available :

- print out the results, see the chapter on printing

- save the data in the PC using the software “Achat OC“, see the chapter

“RS232 interface”

- handwritten documentation of the results.

• Documentation of your instrument settings:

- printout of your instrument settings, see the chapter “GLP/Tools - Settings - Show settings”.

• Switch off the OxiTop

• Loosen the 4 screws underneath the housing using a Phillips screwdriver (see

figure

• Place the controller on the lower case.

• Remove the upper case and put it down to the right next to the lower case with

the display downwards (see figure

• Remove the supply battery holder from the fixing in the lower case and turn it around.

• Remove the empty data backup battery.

• Set the upper case on top of the lower case, turn the controller and tighten the

housing screws using the screwdriver (see figure

• Switch on the controller. The message “Please set the system clock!“ appears. This message is a reminder and should be acknowledged by .

• The battery change was successful if no error message appears concerning the data backup battery.

• Enter the current date/time and your settings.

• Insert the new data backup battery (1 piece, lithium battery, CR2430,

e.g. WTW type Batt/OxiTop Ensure that the battery is the right way round! The positive pole must be at the top.

-OC100 controller.



, order no. 209 012)

Power Supply

OxiTop®-C measuring head

Supply batteries

Batteries: 2 x lithium batteries, CR2430, e.g. WTW type Batt/OxiTop order no. 209 012 These batteries ensure the energy supply of the OxiTop Run time: typically 2 years

The OxiTop

-C measuring head does not require any batteries for data backup.

Supply batteries status messages

- are given on starting the measuring head via the OxiTop display messages and a signal tone

- can be called up in the menu “GLP/Tools - Check -

Display message

Batteries LoBat !

start

Note / Meaning

The instrument can still be safely operated within the specifications. The running reserve of the instrument is still sufficient for the measurement to be started. Please obtain new batteries and replace the old ones!

Battery/ies empty!

- New

- Stop

start

The instrument can no longer be used. The supply batteries must be replaced by new ones. It is possible to start a new measuring head or to complete a sample start.

-C measuring head.

OC100 controller by

info”.



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Power Supply

Changing the supply battery

• Change the battery after the measurement has been terminated.

- Press in the snap-action holder.

- Open the upper case.

- Remove the batteries.

- Insert new batteries (2 x lithium batteries, CR2430, e.g. WTW type

Batt/OxiTop way round!

- Insert the upper case with the lug in the locking pin (lower case).

Caution! Do not crush the cable connection!

- Close the upper case (let the hook snap into place).

Check if the battery change was successful: “Batteries: OK“ must appear in “GLP/Tools - Check -

The batteries have no effect on the data integrity of the OxiTop

Disposing of the batteries



, order no. 209 012). Ensure the batteries are inserted the right

info”.

-C

Properly dispose of used batteries at a battery collection point.

What to do if...?

Display messages

Messages

already

used !

Set system

clock !

defective !

No samples

available !

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Explanations, causes and problem solutions

Cause:

• The selected measuring head has already been started for a measurement and can only be used for a new measurement after the current measurement is finished and the measurement data completely retrieved.

Problem solutions:

• Select new and free measuring head or

• Stop

start or

• Release measuring head (chapter “Maintenance - Release/reset”)

Cause: Data backup battery was changed! Problem solution: Set the clock (see the chapter “GLP/Tools - Settings Date/Time”).

Causes: The selected measuring head is defective. Please send it to WTW. Problem solutions:

• Select new and free measuring head or

• Stop

start

Cause: There are no finished samples stored in the sample management. Problem solution:

• Transfer the unfinished samples of the sample management to “finished” status. To do this, the measurement data of all measuring heads must be called up and evaluation and documentation of the measurement data of the finished samples performed.

• If the measurement data of the measuring heads is not yet finished, no instant remedy is possible! The memory capacity limit of the instrument has been reached.

Messages

No active

Cal test

(example date)

due on 18.07.97!

What to do if...?

Explanations, causes and problem solutions

Causes:

• No measurement has been started.

• The measuring heads are placed at another location

(e.g. other levels in the incubator, other incubators, further storage locations).

• The measuring heads have no optical contact with the controller:

- Distance too great, angular position imprecise

- IR window of the measuring head averted from the controller

- Shading by other objects.

• Controller defective

Problem solutions:

• Modification of the distances or angle

• Search other storage locations

• Check controller

• Test the measuring heads (see section

“Requirements/Problems“)

Causes

• GLP is switched on

• The test resource monitoring is due within the

measuring time of measurement for the selected measuring head.

• Date/time is not correctly set in the controller.

Problem solutions

• Use another free measuring head that has been checked

• Start the due measuring head with the Cal test (see chapter “Check - Cal test”)

• Switch off GLP and start measuring head (if your laboratory practice permits).

• Set the date/time in the controller.

Messages

Lack of memory possible !

Memory for

free ! ContinueÃ

Memory lack !

No ready samples autom.

erasable!

What to do if...?

Explanations, causes and problem solutions

Cause: This message is used as information in the standard BOD operating mode. There is a lot of sample data stored in the sample management. The memory is almost full. It is still possible to start a sample as a parallel sample start with 11 measuring heads (the number 11 an example, possible numbers: 1...11 Problem solution, if necessary:

• Erase finished samples: Chapter “GLP/Tools - Maintenance- Erase finished samples”

• Alternative: Changing the instrument setting to automatic erasing of finished samples: Chapter “GLP/Tools - Settings - Memory - Erase: auto"

Cause: The instrument works with the setting, Erase memory “auto“ (automatically). The memory is full and only unfinished samples are stored in the sample management. The instrument can only erase finished samples automatically. Problem solution:

• Transfer the unfinished samples of the sample management to the “finished“ state.

• To do this, call up the measurement data of all measuring heads and perform the evaluation and documentation for the measurement data of the finished samples.

• If the measurement data of the measuring heads is not yet finished, no instant remedy is possible! The memory capacity limit of the instrument has been reached.

used here is

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What to do if...?

Messages

Memory lack !

Erase finished sample/s!

undef.

(Display in the curve presentation or sample statistics)

Explanations, causes and problem solutions

Cause:

• The instrument is working with the “manual“ setting of Erase memory. The memory is full.

Problem solutions:

• Transfer the unfinished samples of the sample management to “finished“ status

• Call up the measurement data of all the measuring heads

• Perform evaluation and documentation of the measurement data of finished samples.

• Erase finished samples from the sample management: Chapter “GLP/Tools - Maintenance- Erase finished samples”

Note: Only the finished samples in the sample management are erased.

Causes:

• The selected measurement range has been exceeded.

• The sample filled was too warm.

• The AutoTemp function is switched off.

• The sample filled was very cold and has a low

consumption behavior (smaller BOD value).

• The system is not sealed (bottle internal pressure = atmospheric pressure).

Avoiding the problem:

• Select the correct measurement range (see WTW application report).

• Pretemper the sample more precisely.

• Switch on the AutoTemp function (only effective for

measuring times longer than one day).

• Check the system for leaks by means of visual checks: Are the sealing surfaces of bottle and measuring head clean and fault-free? Are there cracks in the sleeve, bottle or measuring head?

• Perform “Cal test“ (see chapter “Check”).

• Check the incubator temperature.

Messages

10 of 12

called up

(sample message, no. is variable)

0 of 19

called up

(sample message, no. is variable)

What to do if...?

Explanations, causes and problem solutions

Cause:

• Two measuring heads have not been found by the controller

• The measuring heads are positioned at another location, e.g. other levels in the incubator, other incubators, further storage positions

• The measuring heads have no optical contact with the controller:

- Distance too great, angular position imprecise

- IR window of the measuring head averted from the controller

- Shading by other objects.

• The missing measuring heads are defective

Problem solutions:

• Modification of the distances or angle

• Search other storage locations

• Search for and check measuring heads (see section

“Requirements/Problems“)

Cause:

• No measuring head has been found by the controller.

• The measuring heads are positioned at another location,

e.g. other levels in the incubator, other incubators, further storage positions

• The measuring heads have no optical contact with the controller.

- Distance too great, angular position imprecise

- IR window of the measuring head averted from the controller

- Shading by other objects.

• The controller is defective.

Problem solutions:

• Modification of the distances or angle

• Search other storage locations

• Check the controller (section “Requirements/Problems“)

88 BA31114e5 07/2006 BA31114e5 07/2006 89

What to do if...?

Requirements / Problems

No or missing samples in the sample management

At the beginning of the measurement, no measurement curves are displayed

Measurement curves does not emerge from the origin

No measurement curves are displayed although the measurement has already been running for an extended period of time

Search for free measuring heads for a new measurement

Procedure / Problem solutions

Cause:

• You started samples in the operating mode „BOD Standard“. Then you switched over to the operating mode „BOD Routine“.

Problem solution:

• Switch the instrument to the operating mode „BOD- Standard“ (see chapter GLP/Tools - Settings - Operating mode).

Causes:

• The sample filled and started was too cold.

• AutoTemp function is switched off.

Problem solution:

• Temper the sample more precisely.

• Switch on the AutoTemp function (only effective for

Causes:

• No data was retrieved from the measuring heads.

• The AutoTemp phase is still running

Problem solution:

• Call up the data of the measuring heads.

• Wait for the end of the AutoTemp phase then start the

Procedure: Perform the function “Show free (see chapter “GLP/Tools”). The controller causes the free measuring positions to flash for 5 seconds.

measuring times longer than one day).

(see the chapter ”AutoTemp function in greater detail“).

See chapter “Call up all data“.

evaluation.

“

Requirements / Problems

Measuring head unintentionally started for measurement

Measuring head started with incorrect settings

Measuring head is required for another sample

Search for the defective measuring head

A measuring head is missing or is defective.

Requirement: To determine the corresponding sample

What to do if...?

Procedure / Problem solutions

Problem solution The measuring head can be released again through the command Reset/release: Chapter “GLP/Tools The controller guides you further through the operation.

If only one measuring head is stored under the corresponding sample number (in the routine BOD operating mode, this is always the case), the controller automatically erases the sample in the sample management.

Procedure:

• Perform a measuring head reaction test (see above). A measuring head that repetitively shows no reaction to the test is defective.

• If the defective measuring head cannot be established in this way, perform the measuring head check (see “Single check”). In doing this, each individual measuring head must be checked until the defective measuring head has been found.

Procedure: Call up the function “Call up data” from the sample management for each individual unfinished sample. The corresponding sample can be determined by this. For subsequent error handling, see the chapter “Sample management - Call up data”.

- Maintenance - Reset/release “.

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What to do if...?

Requirements / Problems

The precision and sealing of the system sample bottles plus measuring head should be checked

Measuring head check (single check)

(To which running sample does the selected measuring head belong?)

The measuring precision of the measuring head should be checked.

Perform measuring head reaction test

Procedure / Problem solutions

Procedure:

See the chapter “GLP/Tools

Procedure: Single check method: menu “GLP/Tools

• The controller guides you further through the operation.

• All single information on the state of the measuring

• The check can be undertaken at any time without

• If no reaction at all can be determined, the batteries

• The measuring precision cannot be checked with this!

Procedure: Pneumatic test (PT) of the measuring head: see the chapter “GLP/Tools

Procedure:

• Menu “GLP/Tools

• All optically attainable measuring heads must flash for

• The check can be undertaken at any time without

• This test only checks the reaction to commands.

• If no measuring head reacts, the controller should be

- Check - Cal test “

- Check - info”

heads is listed, i.e. the measuring head responds.

affecting the measurement that is running.

should be changed and the measuring head check repeated.

- Check - Pneumatic test”

- Check - Show all ”:

approx. 5 seconds independent of their status.

affecting the measurement that is running.

checked.

Requirements / Problems

Checking the controller Problem solution:

Incorrect time display on being switched on

Procedure / Problem solutions

Controller info

• See the chapter “GLP/Tools All single information on the state of the controller is listed.

• The check can be undertaken at any time without affecting the measurement that is running.

• Perform the command: “Show all “GLP/Tools (The check is used in this case to test the IR interface.) All working measuring heads must flash for 5 seconds.

• Perform a measuring head check - see the chapter “GLP/Tools case to test the IR interface). Here, a working measuring head should supply its status data.

• Keyboard, display and signal tone should show the required reaction.

• Check the function of the clock (prerequisite: clock is available). Switch off the instrument and switch it on again. Time and date each appear showing the current values.

• Check RS232 interface (only required when using the WTW software, ACHAT OC):

- Connect controller to your PC by means of the interface cable AK540/B.

- Switch on controller and start the PC program, ACHAT OC. In doing so, the PC program checks the RS232 interface.

Cause:

• Data backup battery has been changed!

• Summer/winter time change has taken place.

Problem solution: Set the clock (see the chapter “GLP/Tools - Settings Date/Time”)

What to do if...?

- Check - Controller info”

“. See

- Check - Show ”.

- Check - info” (The check is used in this

92 BA31114e5 07/2006 BA31114e5 07/2006 93

What to do if...?

Requirements / Problems

The IR printer does not react

Procedure / Problem solutions

Causes:

• Printer is not switched on.

• The printer has no optical contact with the controller:

- Distance too great, angular position imprecise

- IR window of the measuring head averted from the controller

- Shading by other objects.

• Printer batteries are empty.

• No paper or the wrong paper is loaded.

• Printing is not possible in the operating state of the

controller selected.

• The printer or the controller is defective. Action:

• Switch on the printer

• Establish optical contact

• Check or change the printer batteries. Please read the

• Check in the operating manual of the controller whether

operating instructions of the printer.

printing is possible in the state selected. Note: During printing, the message “Printing active” always appears on the display.

Power supply / Battery status

For battery status messages, see the chapter “Power supply”.

Accessories and spare parts

Accessories

See WTW General Catalog and WTW application reports.

Spare parts

Description Type Order no.

BOD bottle (Sample bottle, amber, contents 510 ml), minimum order 3 pcs

Rubber sleeve, minimum order 3 pcs GK 600 209170

Stirrer bar, minimum order 3 pcs RST 600 209120

Stirrer bar remover REF 600 209130

2 bottles of sodium hydroxide pellets (of 50 g) NHP 600 209140

Nitrification inhibitor NTH 600 209331

Spare batteries (1 set) Batt/OxiTop

Spare OxiTop

-C measuring head OxiTop®-C 208830

PF 600 209100

209012

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Technical Data

OxiTop® OC 100 controller

Measurement ranges 0 ... 40/ 80/ 200/ 400/ 800/ 2000/ 4000 mg/l BOD Run times and data sets Run time of the

measurement

Measurement period

Display LCD graphics display 64 x 128 pixels Power supply Supply batteries: 3 pcs, alkaline (alkaline

Battery run time

Supply battery

Data backup battery

Safety class 3, equipment safety: IEC 1010, EN 61010 part 1 Protection type IP 41 DIN 40050 EMC

-Interference emission

-interference immunity Climate class 2, VDI/VDE 3540 Ambient temperature Storage: -25°C ... +65°C

Dimensions 45 x 100 x 200 mm (H x B x T) Weight approx. 390 g Test certificates CE, TÜV, UL, CUL(UL/CSA) Warranty 1 year

manganese), Size: Mignon, AA, AM3, LR6 Data backup battery: 1 x lithium battery, CR2430, WTW order no. 209 012

> 1000 switchings in case of normal use; (the instrument has an automatic shut off) Typically 4 years

EN 50081-1, FCC Class A EN 50082-1

Operation: +5°C ... +40°C

0.5 hours 180

1.5 hours 270

2.5 hours 300

3.5 hours 315

4.5 hours 324

5.5 hours 330

all whole hours

(1, 2, 3 to 23 hours)

all whole days

(1, 2, 3 to 99 days)

Data records

360

Technical Data

OxiTop

Measuring principle manometric by means of a pressure measuring head

Measured of BOD

Pressure range

Accuracy ± 1% of measured value ± 0.7 % of the measuring

Resolution 1 hPa

Display LED pilot lamps

Power supply 2 x lithium batteries, CR2430, e.g. WTW type

Battery run time Typically 2 years, in case of normal use

Safety class 3, equipment safety: IEC 1010, EN 61010 part 1

Protection type IP 54 DIN 40050

EMC

-Interference emission

-interference immunity

Climate class 2, VDI/VDE 3540

Ambient temperature Storage: -25°C ... +65°C

Dimensions H: 70 mm, ∅∅∅∅ 70 mm

Weight 95 g

Test certificates CE, TÜV, UL, CUL(UL/CSA)

Warranty 1 year

-C measuring head

(oxygen drift according to DIN 38409 T 52)

500 ... 1350 hPa (mbar).

range

Batt/OxiTop

EN 50081-1, FCC Class A EN 50082-1

Operation: +5°C ... +50°C



, order no. 209 012)

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Technical Data

BA31114e05 07/2006 1

Supplement to the operating manual

System OxiTop® Control

Controller

OxiTop

Measuring heads

OxiTop®-C

This sheet is a supplement to the present operating manual and contains the chapter “Restore data“ and some notes.

Always keep this sheet together with the operating manual.

OC100

Supplement to the operating manual

Restore data

In case you lost your controller or your controller is defective, the “Restore data“ function facilitates to restore the data of running measurements using a new or another controller. This means the data are not lost.

To perform this function the memory of the controller must be absolutely empty. The data of each single measuring head are restored one after the other in a sequence. It is not possible to leave this function and to continue it afterwards because then the memory would no longer be empty.

Use to select the “Restore data“ submenu.

Confirm the restoration for one measuring head and hold the controller to the measuring head. Contact selection:

The controller displays the serial number of the selected measuring head.

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Xylem OC100 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual