Metrohm 766IC User Manual

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CH-9101 Herisau/Switzerland Tel. ++41 71 353 85 85 Fax ++41 71 353 89 01 Internet www.metrohm.ch E-Mail info@metrohm.ch

766 IC Sample Processor

DES FUSIBLES DE 250V

USE ONLY WITH 250V

100-120V

UNIQUEMENT AVEC

FUSES/EMPLOYER

MetrohmMetrohm

220-240V

8.766.1003 Instructions for Use

30.06.1998 / dö

Table of contents

1 Introduction....................................................................................... 1

1.1 Instrument description................................................................... 1

1.2 Parts and controls........................................................................... 2

1.3 Information about the Instructions for Use................................ 5

1.3.1 Organization ............................................................................ 5

1.3.2 Notation and pictograms ........................................................ 6

1.4 Support documentation .................................................................. 7

1.4.1 Application Bulletins................................................................ 7

1.4.2 Application Notes.................................................................... 7

1.5 Safety notes....................................................................................... 7

1.5.1 Electrical safety ....................................................................... 7

1.5.2 General safety rules ................................................................ 8

2 Installation......................................................................................... 9

2.1 Setting up the instrument............................................................... 9

2.1.1 Packaging................................................................................ 9

2.1.2 Check....................................................................................... 9

2.1.3 Location................................................................................... 9

2.1.4 Arrangement of the instruments ............................................. 9

2.2 Mains connection........................................................................... 10

2.2.1 Setting the mains voltage ..................................................... 10

2.2.2 Fuses ..................................................................................... 11

2.2.3 Mains cable and mains connection...................................... 11

2.2.4 Switching the instrument on/off ............................................ 11

2.3 Attaching the accessories............................................................ 12

2.3.1 Connecting the swing head.................................................. 12

2.3.2 Connecting the keyboard ..................................................... 12

2.3.3 Installing the plug cover........................................................ 12

2.3.4 Installing the splash protection............................................. 13

2.3.5 Installing the needle .............................................................. 13

2.3.6 Placing the sample rack........................................................ 14

2.3.7 Adjusting the sample rack .................................................... 14

2.3.8 Tubing connections 766 – 733 ............................................. 16

2.3.9 Tubing connections 766 – 754 ............................................. 18

2.3.10 Fixing tubing and cables..................................................... 18

766 IC Sample Processor

Table of contents

3 Operating tutorial..................................................................... 27

2.4 Connection of devices to the remote interface........................ 19

2.4.1 General information on remote interface...............................19

2.4.2 Connection cables .................................................................19

2.4.3 IC system without suppression .............................................20

2.4.4 IC system with suppression with 766 as "Master".................20

2.4.5 IC system with suppression with PC as "Master"..................21

2.4.6 IC system for simultaneous anion/cation determination ......21

2.4.7 IC system with sample dialysis..............................................22

2.5 Connection of devices to the RS232 interface......................... 23

2.5.1 General information on RS232 interface ...............................23

2.5.2 Connection of a printer ..........................................................24

3.1 Prerequisites / Preparations......................................................... 27

3.2 Configuration .................................................................................. 28

3.3 Rack configuration......................................................................... 29

3.4 Methods............................................................................................ 31

3.5 "Tracing".......................................................................................... 31

4 Operation.......................................................................................... 35

4.1 Fundamentals of operation.......................................................... 35

4.1.1 Display....................................................................................35

4.1.2 Keyboard................................................................................36

4.1.3 Overview of key functions......................................................37

4.1.4 Instrument dialog ...................................................................46

4.1.5 Data entry ...............................................................................48

4.1.6 Text entry................................................................................49

4.2 Basic settings ................................................................................. 51

4.2.1 Configuration – <CONFIG> key...........................................51

4.2.2 Locking keyboard functions ..................................................55

4.3 Methods............................................................................................ 57

4.3.1 Structure of a method ............................................................57

4.3.2 Method parameters – <PARAM> key..................................58

4.3.3 Programming of sequences ..................................................60

4.3.4 LEARN mode..........................................................................61

4.3.5 TRACE function......................................................................61

4.3.6 Commands for sequences ....................................................62

4.3.7 Process control ......................................................................67

4.3.8 User defined methods ...........................................................68

4.3.9 POWERUP method................................................................69

4.4 Manual operation............................................................................ 70

4.4.1 Turning the sample rack / Positioning the samples..............70

4.4.2 Moving the lift .........................................................................70

766 IC Sample Processor

Table of contents

4.4.3 Setting the sample position .................................................. 71

4.4.4 Pump control......................................................................... 71

4.4.5 Display interface signals ....................................................... 71

4.4.6 Interface control..................................................................... 72

4.4.7 Print out reports..................................................................... 73

4.5 Sample racks................................................................................... 75

4.5.1 Standard rack (6.2041.430) .................................................. 75

4.5.2 Magnetic code....................................................................... 75

4.5.3 Rack data .............................................................................. 76

4.5.4 Special beakers..................................................................... 78

4.6 Standard methods.......................................................................... 79

4.6.1 Method "PC" .......................................................................... 79

4.6.2 Method "PC Seg"................................................................... 80

4.6.3 Method "SP"........................................................................... 81

4.6.4 Method "SP Seg" ................................................................... 82

4.6.5 Method "An Cat" .................................................................... 83

4.6.6 Method "AnCatSeg"............................................................... 84

4.6.7 Method "Preconc".................................................................. 86

4.6.8 Method "Dialysis"................................................................... 87

5 Interfaces.........................................................................................89

5.1 Remote interface............................................................................. 89

5.1.1 Pin assignment of the remote socket:.................................. 89

5.1.2 Functional characteristics ..................................................... 90

5.2 RS232 interface............................................................................... 93

5.2.1 General rules for remote control........................................... 93

5.2.2 Call-up of objects .................................................................. 94

5.2.3 Trigger.................................................................................... 95

5.2.4 Status messages................................................................... 96

5.2.5 Error messages..................................................................... 97

5.2.6 Remote control commands .................................................. 98

5.2.7 Data transmission protocol................................................. 105

5.2.8 Handshake .......................................................................... 106

5.2.9 Pin assignment.................................................................... 109

5.2.10 RS232 error rectification.................................................... 110

6 Appendix.........................................................................................111

6.1 Error messages............................................................................. 111

6.2 Technical data............................................................................... 113

6.3 Maintenance and servicing......................................................... 115

766 IC Sample Processor

6.3.1 Maintenance by Metrohm service ...................................... 115

6.3.2 Care of the unit.................................................................... 115

6.3.3 Replacing the pump tubing ................................................ 116

III

Table of contents

6.4 Diagnosis....................................................................................... 117

6.4.1 General Information .............................................................117

6.4.2 Preparing the instrument .....................................................118

6.4.3 Working memory (RAM).......................................................119

6.4.4 Display..................................................................................119

6.4.5 Keyboard ..............................................................................120

6.4.6 Remote interface..................................................................121

6.4.7 RS232 interface....................................................................122

6.4.8 External Bus interface ..........................................................122

6.4.9 Beeper ..................................................................................123

6.4.10 Rack code recognition.......................................................123

6.5 Initialize data memory ................................................................. 124

6.6 Validation / GLP............................................................................ 126

6.7 Warranty and conformity............................................................ 127

6.7.1 Warranty ...............................................................................127

6.7.2 EU Declaration of conformity...............................................128

6.7.3 Certificate of conformity and system validation ..................129

6.8 Standard equipment .................................................................... 130

6.9 Optional accessories................................................................... 132

6.10 Index ............................................................................................. 133

List of figures

Fig. 1: Side view of the 766 IC Sample Processor ...................................................2

Fig. 2: Rear of the 766 IC Sample Processor .......................................................... 4

Fig. 3: Setting the mains voltage.......................................................................... 11

Fig. 4: Keyboard connection ...............................................................................12

Fig. 5: Installing the splash protection.................................................................. 13

Fig. 6: Needle installation.................................................................................... 14

Fig. 7: Sample rack placing................................................................................. 14

Fig. 8: Adjusting the sample rack ....................................................................... 15

Fig. 9: Installing the pump tubing ........................................................................17

Fig. 10: Opening chain links.................................................................................. 18

Fig. 11: Interconnection with IC system without suppression...................................20

Fig. 12: Interconnection with IC system with suppression with 766 as "Master"......... 20

Fig. 13: Interconnection with IC system with suppression with PC as "Master".......... 21

Fig. 14: Interconnection with IC system for anion/cation system .............................. 21

Fig. 15: Interconnection with IC system with dialysis............................................... 22

Fig. 16: Connection possibilities for the RS232 interface ........................................ 23

Fig. 17: Schematic representation of the instrument dialog .....................................47

Fig. 18: Installation of the preconcentration column ............................................... 86

766 IC Sample Processor

1 Introduction

1.1 Instrument description

The 766 IC Sample Processor can be used for automating ion chromatographic determinations, especially in combination with the Metrohm IC system instruments. The 127 sample tubes with a volume of up to 11 mL are arranged on the sample rack in three rows, which guarantees easy access and unrestricted programming. Two additional rinsing positions allow sample introduction free from crosscontamination even with widely varying sample matrices. If the 766 IC Sample Processor is used together with the 732 IC Detector and the 733 IC Separation Center, various injections are possible from a single sample tube, depending on the sample size. Sample tubes made of polypropylene are standard. To protect the samples from external contamination, the tubes can be hermetically sealed.

1.1 Instrument description

Sample introduction from the 766 IC Sample Processor is achieved by means of its integrated peristaltic pump. The sample is conveyed by the pump through the capillary into the sample loop of the injector located within the 733 IC Separation Center. A steel needle for sealed or a PEEK needle for open sample tubes can be used alternatively. Owing to the relatively large volume of the sample tubes the 766 IC Sample Processor can also be used for applications with enrichment columns, dialysis (with the 754 Dialysis Unit) or simultaneous anion/cation determinations.

The sequences for the processing of each sample are freely definable within broad boundaries. The same is true for the start and final sequences that are executed once either before or at the end of a sample series. With the help of the LEARN mode, which is provided for creating process methods, methods can be created easily and stored as user methods.

Preprogrammed standard methods for the most common modes of operation allow to use the 766 IC Sample Processor directly, with only little programming effort. The time frame can be given by the IC Sample Processor; alternatively, the whole sequence can be managed by the «IC Metrodata» remote control software via the «Queue» (sample table). The RS232 interface built in as standard allows remote control of the IC Sample Processor from a PC.

766 IC Sample Processor

1 Introduction

1.2 Parts and controls

55 66 77 88 99

Always install splash protection!

1010

1111

1212

1313

1414

1515

1616

1717

1111

2828

2727

2626

2525

2424

MetrohmMetrohm

DES FUSIBLES DE 250V

USE ONLY WITH 250V

100-120V

UNIQUEMENT AVEC

FUSES/EMPLOYER

220-240V

Always install plug cover!

Fig. 1: Side view of the 766 IC Sample Processor

1818

1919

2020

2121

2222

2323

766 IC Sample Processor

1.2 Parts and controls

11 Splash protection

(6.2751.040)

Must be installed always in operation!

22 Needle

Steel needle (6.2624.000) or PEEK needle (6.1835.000)

33 Lift

With swing head attached

44 Needle holder

(attached)

55 PEEK compression fitting

(4.766.4070)

For peek capillary 88

66 PEEK compression fitting

(6.2744.010)

1515 Contact pressure lever

For adjusting the contact pressure

1616 Holding clamp

For locking the tubing cartridge into place

1717 Snap-action lever

For releasing the tubing cartridge

1818 PEEK capillary (6.1831.060; 1 m)

For conveying the sample to the injection valve of the 733 IC Separation Center

1919 Tower

2020 PE bottle (6.1608.080; 300 mL)

77 Swing head

(attached)

88 PEEK capillary (6.1831.050;

40 cm)

Connection needle – pump tubing

99 Guide chain

For fixing tubings and cables

1010 Pump tubing (6.1826.040)

For conveying the sample

1111 Screw thread for splash

protection

1212 Mounting pin

For attaching the tubing cartridge

2121 Connection cable for swing head

Attached, incl. branch plug for remote connection

2222 Remote connection

2323 Plug cover (6.2752.010)

2424 Fuse holder

Changing the fuses, see section 2.2.2

2525 Mains connection plug

Mains connection, see section 2.2.3

2626 Mains switch

For switching the instrument on/off: I = ON 0 = OFF

1313 Tubing cartridge (6.2755.000)

For 6.1826.0X0 pump tubing

1414 Pump drive

Roller head with contact rollers

766 IC Sample Processor

2727 Sample rack (6.2041.430)

2828 PP sample tube (6.2743.050)

(can be sealed with 6.2743.060 PE caps)

1 Introduction

2929

1313

2929

2020

2323

2929

1919

2929

2828

2020

3030

Type 1.766.

RS 232 Keyboard

Made by Metrohm Herisau Switzerland

3131

3232

Fig. 2: Rear of the 766 IC Sample Processor

11 Splash protection (6.2751.040)

Must be installed always in operation!

99 Guide chain

For fixing tubings and cables

1313 Tubing cartridge (6.2755.000)

For 6.1826.0X0 pump tubing

1919 Tower 3030 Serial number 2020 PE bottle (6.1608.080; 300 mL) 3131 Keyboard connection 2323 Plug cover (6.2752.010) 3232 RS232 connection

2727 Sample rack (6.2041.430)

2828 PP sample tube (6.2743.050)

(can be sealed with 6.2743.060 PE caps)

2929 Mounting screws for splash

protection

766 IC Sample Processor

1.3 Information about the Instructions for Use

Please read through these Instructions for Use carefully before operating the 766 IC Sample Processor. The Instructions for Use contain information and warnings to which the user must pay attention in order to assure safe operation of the instrument.

1.3.1 Organization

These 8.766.1003 Instructions for Use for the 766 IC Sample Processor provide a comprehensive overview of the installation, startup procedure, operation, fault rectification and technical specifications of this instrument. The Instructions for Use are organized as follows:

Section 1 Introduction

Description of the instrument, parts and controls, safety notes

Section 2 Installation

Mains connection, attachment of accessories, connection of external devices

Section 3 Operating tutorial

Introduction to the operation

Section 4 Operation

Detailed description of display, keyboard, methods, manual operation, sample racks, standard methods

Section 5 Interfaces

Remote interface, RS232 interface and remote control language

Section 6 Appendix

Error messages, technical data, maintenance and servicing, diagnosis, warranty, declaration of conformity, standard equipment, optional accessories, index

To find the information you require about the instruments please use either the Table of contents or the Index at the back. The 8.766.1013 Quick Reference Guide is suitable for use as a reference work for daily use as it explains the most important parameters and key functions.

766 IC Sample Processor

1 Introduction

1.3.2 Notation and pictograms

The following notations and pictograms (symbols) are used in these Instructions for Use:

<PUMP> Switch or key

1515 Part or control of 766

8989 Part or control of 732/733

2626 Part or control of 754

'rack number' Parameter or entry value

at 766 IC Sample Processor

******** counter 0/127 PUMP- ready

Display

Text in keyboard display of 766 IC Sample Processor

Hazard

This symbol draws attention to a possible danger to life or injury if the associated directions are not followed correctly.

Warning

This symbol draws attention to possible damage to instruments or instrument parts if the associated directions are not followed correctly.

Caution

This symbol marks important information. Read these directions before continuing.

Comment

This symbol marks additional information and tips.

766 IC Sample Processor

1.4 Support documentation

1.4.1 Application Bulletins

The «Application Bulletin» is a collection of analytical methods, application examples and literature references. Of Metrohm's approximately 200 Application Bulletins, 34 currently refer to ion chromatography. All these Application Bulletins are available on request free of charge from your Metrohm supplier.

You will find an updated list of the Application Bulletins at any time under «http://www.metrohm.ch».

1.4.2 Application Notes

The «Application Notes» present application information in concentrated form, i.e. on maximum 2 pages. There are currently 88 Application Notes (in English) in the field of ion chromatography. You can order these free of charge from your Metrohm supplier or view them in the Internet under «http://www.metrohm.ch» and copy them from there.

1.4 Support documentation

1.5 Safety notes

1.5.1 Electrical safety

While electrical safety in the handling of the 766 IC Sample Processor is assured in the context of the specifications IEC 1010-1 (protection class 1, degree of protection IP40), the following points should be noted:

• Mains connection

Set the mains voltage and check the mains fuse and mains connection in accordance with the instructions in section 2.2.

• Opening the 766 IC Sample Processor

To avoid all danger of coming into contact with live components do not open the instrument or remove any parts when the 766 IC Sample Processor is connected to the power supply. Always disconnect the instrument from all voltage sources before you open it and ensure that the mains cable is disconnected from mains connection 2525 !

766 IC Sample Processor

1 Introduction

• Protection against static charges

Electronic components are sensitive to static charging and can be destroyed by discharges. Before you touch any of the components inside the 766 IC Sample Processor, you should earth yourself and any tools you are using by touching an earthed object (e.g. housing of the instrument or a radiator) to eliminate any static charges which exist.

1.5.2 General safety rules

• Install splash protection

To avoid any danger of injury by the needle, the splash protection must always be installed when operating the 766 IC Sample Processor!

• Install plug cover

To prevent any contamination of the mains and remote connection by spilled solvents or chemicals, the plug cover must always be installed when operating the 766 IC Sample Processor !

• Do not use caps together with the PEEK tube

If you are using the 6.1835.000 PEEK needle, the sample tubes may not be sealed with caps because they cannot be pierced by the PEEK needle and the needle may be damaged thereby.

• Handling of solvents

Check the pump tubing and all input and output leads periodically for possible leaks. Follow the relevant instructions regarding the handling of flammable and/or toxic solvents and their disposal.

• Regular exchange of pump tubings

Pump tubings constitute consumable material and must be replaced from time to time (see section 6.3.3). Suitable measures must be taken so that any leak which might occur in the pump tubing or connections during unattended operation will cause no damage (collection device for any liquid which may leak out).

766 IC Sample Processor

2 Installation

2.1 Setting up the instrument

2.1.1 Packaging

The 766 IC Sample Processor is supplied together with the separately packed accessories in special packagings containing shock-absorbing foam linings designed to provide excellent protection. The actual instrument is packed in an evacuated polyethylene bag to prevent the ingress of dust. Please store all these special packagings as only they can assure damage-free transport of the instrument.

2.1 Setting up the instrument

2.1.2 Check

After receipt, immediately check whether the shipment is complete and undamaged (compare with delivery note and list of accessories in sec-

tion 6.8). In the case of transport damage, see instructions in section

6.7.1 "Warranty".

2.1.3 Location

Position the instrument in the laboratory at a location convenient for operation, free from vibrations and protected against a corrosive atmosphere and contamination by chemicals.

Do not operate the 766 IC Sample Processor without splash protection 11 and plug cover 2323 being installed!

2.1.4 Arrangement of the instruments

To make the connection between needle 22 and the injection valve at the 733 IC Separation Center with the peek capillary 1818 (length 1 m) supplied, position the 766 IC Sample Processor immediately on the left side of the IC system.

766 IC Sample Processor

Take precautions to ensure that any leaks from pump tubings or connections cannot cause more damage.

2 Installation

2.2 Mains connection

Follow the instructions below for connecting to the power supply. If the instrument is operated with the mains voltage set wrongly and/or wrong mains fuse there is a danger of fire!

2.2.1 Setting the mains voltage

Before switching on the 766 IC Sample Processor for the first time, check that the mains voltage set on the instrument (see Fig. 2) matches the local mains voltage. If not, reset the mains voltage on the instrument as follows:

Disconnect mains cable

Disconnect mains cable from mains connection plug 2525 of the 766 IC Sample Processor.

Remove fuse holder

Using a screwdriver, loosen fuse holder 2424 beside the mains connection 2525 and take out completely.

Check fuse

Carefully take the fuse installed for the desired mains voltage out of fuse holder 2424 and check its specifications (the position of the fuse in the fuse holder is marked by the white arrow imprinted next to the mains voltage range):

100……120 V 0.5 A (slow-blow) Metrohm-Nr. U.600.0013 220……240 V 0.25 A (slow-blow) Metrohm-Nr. U.600.0010

Insert fuse

Change fuse if necessary and reinsert in fuse holder 2424.

Install fuse holder

Depending on the desired mains voltage, insert fuse holder 2424 in the 766 IC Sample Processor so that the corresponding mains voltage range can be read normally and the adjacent white arrow points to the white bar imprinted on the right side of the fuse holder (see Fig. 3).

766 IC Sample Processor

2.2 Mains connection

100 – 120 V

2626

2525

2424

2.2.2 Fuses

220 – 240 V

220

- 240 V

- 120 V

100

- 120 V

- 240 V

220

2424 Fuse holder

2525 Mains connection

plug

2626 Mains switch

Fig. 3: Setting the mains voltage

One of the two fuses 0.5 A/slow-blow for 100…120 V or 0.25 A/slowblow for 220…240 V is installed in fuse holder 2424 of the 766 IC Sample Processor as standard.

Ensure that the instrument is never put into operation with fuses of another type, otherwise there is danger of fire!

For checking or changing fuses, proceed as described in section 2.2.1.

2.2.3 Mains cable and mains connection

Mains cable

The instrument is supplied with one of three mains cables

• 6.2122.020 with plug SEV 12 (Switzerland, …)

• 6.2122.040 with plug CEE(7), VII (Germany, …)

• 6.2133.070 with plug NEMA 5-15 (USA, …)

which are three-cored and fitted with a plug with an earthing pin. If a different plug has to be fitted, the yellow/green lead (IEC standard) must be connected to protective earth (protection class 1).

Any break in the earthing inside or outside the instrument can make it a hazard!

Mains connection

Plug the mains cable into mains connection plug 2525 of the 766 IC Sample Processor (see Fig. 3).

2.2.4 Switching the instrument on/off

The 766 IC Sample Processor is switched on and off using mains switch 2626.

766 IC Sample Processor

2 Installation

2.3 Attaching the accessories

For attaching the accessories at the 766 IC Sample Processor, proceed in the order described below.

2.3.1 Connecting the swing head

Plug in the branch plug of the connection cable 2121 permanently at- tached to the swing head into the remote connection socket at the 766 IC Sample Processor and screw it onto this connection using a screwdriver (see Fig. 1).

2.3.2 Connecting the keyboard

Connecting the keyboard

Connect the 6.2142.010 keyboard to the keyboard connection 3131 "Keyboard". For disconnection, press the plug together slightly on both sides.

Switch on instrument

Switch on the 766 IC Sample Processor with mains switch 2626. The keyboard display lights up. The instrument is initialized and the lift is raised completely.

2.3.3 Installing the plug cover

Fig. 4: Keyboard connection

To prevent any contamination of the mains and remote connection by spilled solvents or chemicals, the 6.2752.010 plug cover must always be installed when operating the 766 IC Sample Processor !

Install the plug cover 2323 in the corresponding guide groove above mains connection plug 2525 and remote connection 2222 (see Fig. 1 and Fig. 2).

766 IC Sample Processor

2.3.4 Installing the splash protection

To avoid any danger of injury by the needle, the 6.2751.040 splash protection must always be installed when operating the 766 IC Sample Processor!

Remove holding screws

Remove the holding screws 2929 and the washer mounted on the screw threads 1111 at tower 1919 using the 6.2621.100 Allen key.

Remove protective film from splash protection

Remove the plastic film glued on both sides of the splash protection 11.

Install splash protection

Attach splash protection 11 onto the screw threads 1111 at the tower 1919 and fix it with the holding screws 2929 and the washer using the 6.2621.100 Allen key.

2.3 Attaching the accessories

11 Splash protection

(6.2751.040)

2929 Holding screws

2.3.5 Installing the needle

Remove PEEK compression fitting 5

Remove PEEK compression fitting 55 screwed onto the needle holder 44.

Insert needle

Insert needle 22 (6.2624.000 steel needle or 6.1835.000 PEEK needle) completely into the opening of the needle holder 44.

2929

Fig. 5: Installing the splash protection

766 IC Sample Processor

Fix needle

Tighten compression fitting 55 in needle holder 44 by hand (never use tools!).

2 Installation

22 Steel needle (6.2624.000) or

PEEK needle (6.1835.000)

44 Needle holder

55 PEEK compression fitting

(4.766.4030)

66 PEEK compression fitting

(6.2744.010)

77 Swing head

88 PEEK capillary (6.1831.050)

If you are using the 6.1835.000 PEEK needle, the sample tubes may not be sealed with caps because they cannot be pierced by the PEEK needle and the needle may be damaged thereby.

Fig. 6: Needle installation

2.3.6 Placing the sample rack

Place sample rack

Place sample rack 2727 on the turntable of the 766 IC Sample Processor acc. to Fig. 7.

Read magnetic code

Press <RESET> to move the rack to the home position, in which the magnetic code can be read (details see section

4.5).

2.3.7 Adjusting the sample rack

If a new sample rack is placed on the 766 IC Sample Processor for the first time, it must be adjusted on a rack position in the middle row (example: position 37) as follows:

Fig. 7: Sample rack placing

Place sample rack

Place empty sample rack 2727 on the 766 IC Sample Processor and press <RESET> (see section 2.3.6).

766 IC Sample Processor

2.3 Attaching the accessories

22 Steel neddle

(6.2624.000) or PEEK needle (6.1835.000)

2727 Sample rack

(6.2041.430)

3232 Adjusting position 37

3333 Adjusting screw

Move to adjusting position

Press <MOVE>, enter number '37' and confirm with <ENTER>. Sample rack and swing head are turned until needle 22 is above the adjusting position 3232 (position 37: first opening of the middle row).

Check needle position

• Press <ê> until the needle 22 is ca. 1 cm above the upper

level of the sample rack 2727.

• Check needle position: If the needle 2 cannot be lowered

unhindered through the upper hole of the adjusting position 32, continue directly with point 4.

• Continue lowering the needle by pressing <ê> until the

needle 22 is ca. 1 cm above the lower level of the sample rack

2727.

• Check needle position: If the needle 2 cannot be lowered

unhindered through the lower hole of the adjusting position 32, continue directly with point 4.

• Lower needle completely by pressing <ê>.

• Check needle position: If the needle 22 is in the middle of the

lower hole, the sample rack must not be adjusted (continue in this case with point 5).

3232

3333

2727

Fig. 8: Adjusting the sample rack

766 IC Sample Processor

Adjust sample rack

• Loosen the three adjusting screws 3333 on the lower level of the

sample rack using the 6.2621.100 Allen key

• Carefully turn the two upper levels of the sample rack 2727 by

hand until the lowered needle 22 is exactly in the middle of the lower hole at the adjusting position 3232.

• Tighten the adjusting screws 3333.

Move to rest position

Press <RESET> to move the sample rack to the rest position.

2 Installation

2.3.8 Tubing connections 766 – 733

For transferring the sample from the 766 IC Sample Processor to the injection valve of the 733 IC Separation Center the following tubing connections must be made:

Pump tubings are consumable material with a lifetime which depends on the contact pressure (see section 6.3.3). This is why the tubing cartridges should be raised completely by loosening snap-action lever 1717 on the right-hand side if the pump is to remain switched off for a considerable length of time (the set contact pressure remains unchanged).

The 6.1826.0X0 pump tubing is made of PVC and must not be used for rinsing with solutions which contain acetone. In such cases, rinse with different pump tubing or a different pump.

Install pump tubing

• Release tubing cartridge 1313 from holding clamp 1616 by

pressing down snap-action lever 1717 and remove from mounting pin 1212 on the 766 IC Sample Processor (see Fig.

1).

• Press contact pressure lever 1515 on the tubing cartridge down

as far as it will go.

• Insert pump tubing 1010 in the tubing cartridge 1313 as shown in Fig. 9. The black-black stopper 3636 must click into the corre- sponding holder on the left-hand side of the tubing cartridge.

• Place the tubing cartridge 1313 on mounting pin 1212 and press down on the right-hand side until snap-action lever 1717 clicks into position on holding clamp 1616. Take care that no kinks are formed in the pump tubing.

Connection needle – pump tubing

• Mount the PEEK compression fitting 66 on one end of the PEEK capillary 88 and the PEEK compression fitting 3434 on the other end.

• Screw the PEEK capillary 88 with the PEEK compression fitting 66 on to the PEEK compression fitting 55 already mounted on needle holder 44 (see Fig. 6).

• Insert PEEK capillary 88 into the guide chain 99 (see Fig. 1 and section 2.3.8).

• Screw a coupling 3535 on to the PEEK compression fitting 3434 at the other end of PEEK capillary 88.

• Push coupling 3535 on to the inlet end of the pump tubing 1010 (see Fig. 6).

766 IC Sample Processor

88 3535 1010 3636 1313 1515 3636 3535 18181717 343466

2.3 Attaching the accessories

Fig. 9: Installing the pump tubing

66 PEEK compression fitting

1717 Snap-action lever

(6.2744.010)

88 PEEK capillary (6.1831.050) 1818 PEEK capillary (6.1831.060)

1010 Pump tubing (6.1826.040) 3434 PEEK compression fitting

(6.2744.010)

1313 Tubing cartridge (6.2755.000) 3535 Coupling (6.2744.030) 1515 Contact pressure lever 3636 Stopper (black-black)

Connection pump tubing – injection valve

• At the 733 IC Separation Center, loosen the rotary nipple

screwed onto the interior side of connection 2222 or 2828.

• Take PTFE suction tubing 8484 (see Fig. 14 and Fig. 15 of the

732/733 Instructions for Use) completely out of connection 2222 or 2828 and unscrew from connection "1" of injection valve 6666.

• Pull the PEEK capillary 1818 through the opening 2222 or 2828 of

the 733 IC Separation Center and screw onto connection "1" of injection valve 6666 using a 6.2744.010 PEEK compression fitting.

• Retighten rotary nipple on the interior side of connection 2222

or 2828 to fix the capillary 1818.

766 IC Sample Processor

Tubing connection injection valve – waste

• Insert 6.2744.020 coupling (from 733 accessories) into con-

nection 2121 or 2727 of the 733 IC Separation Center.

• Screw PTFE suction tubing 8484 onto the 6.2744.020 coupling

attached to connection 2121 or 2727 and lead it into the waste container.

In the case of the 733.0020 IC Separation Center with two injection valves, it is possible to fill both sample loops from the same 766 IC Sample Processor. For this, connection "1" of valve A (outlet of the sample loop) must be connected to connection "2" of valve B (inlet of the sample loop) using a 6.1803.040 PEEK capillary (15 cm).

2 Installation

2.3.9 Tubing connections 766 – 754

If the 766 IC Sample Processor is used for an IC system with sample dialysis (see section 2.4.6), the peristaltic pump at the 754 Dialysis Unit is used for sample conveying instead of the pump at the 766 IC Sample Processor. The tubing connections between the 754 Dialysis Unit 754 and the 733 IC Separation Center 733 have to be made as shown in Fig. 8 of the 754 Instruction for Use. The only change concerns the inlet of the sample, which is installed as follows:

Install a PEEK compression fitting 6.2744.010 on the inlet end of the PTFE tubing 2626 (6.1803.030, see Fig. 8 of the 754 Instruction for Use) connected to the 754 Dialysis Unit and screw it on the PEEK compression fitting 55 (see Fig. 6).

2.3.10 Fixing tubing and cables

In order to fix tubing or cables in the guide chain 99 any chain link may be opened with a screw driver or another appropriate tool.

Fig. 10: Opening chain links

766 IC Sample Processor

2.4 Connection of devices to the remote interface

732/1 Remote

2.4 Connection of devices to the remote interface

2.4.1 General information on remote interface

The branch plug of cable 2121 leading from the swing head (see Fig. 1) is plugged into the 25 pin remote interface (see section 2.3.1). Any external devices can be connected to remote connection 2222 of this branch plug. The 766 IC Sample Processor can be remote controlled via the 8 input lines, the 14 output lines can be used to control external devices.

Before an external device is connected to remote connection 2222, the 766 IC Sample Processor must always be switched off using mains switch 2626!

The pin assignment of the remote interface, its functions, the electrical requirements and conditions are described in section 5.1.

2.4.2 Connection cables

Connecting peripheral instruments to the 766 IC Sample Processor requires Metrohm cables. Otherwise a safe data transmission may not be guaranteed.

Metrohm cables are labeled with the type of the instrument which they may be connected with and optionally with the particular socket. Mind the cable ends.

Example:

766 IC Sample Processor

2 Installation

2.4.3 IC system without suppression

The 766 IC Sample Processor is connected to an IC system without suppression consisting of 732 IC Detector, 733 IC Separation Center and 709 IC Pump as shown in Fig. 11 using the 6.2141.110 cable. With this interconnection the standard methods 'PC', 'PC Seg', 'SP' and

'SP Seg' can be used (see section 4.6).

732

733

6.2125.060 cable

709

766

6.2141.110 cable

Fig. 11: Interconnection with IC system without suppression

2.4.4 IC system with suppression with 766 as "Master"

The 766 IC Sample Processor is connected to an IC system with suppression consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump and either 752 Pump Unit or 753 Suppressor Module as shown in Fig. 12 using the 6.2125.120 adaptor. With this interconnection, in which the 766 IC Sample Processor is the "Master", the standard methods 'SP' and 'SP Seg' can be used (see section 4.6).

6.2125.090 cable

732

733

709

766

6.2125.120 adaptor

6.2125.090 cable

6.2143.210 cable

6.2125.090 cable

6.2141.110 cable

6.2125.060 cable

752

753

6.2143.200 cable

Fig. 12: Interconnection with IC system with suppression with

766 as "Master"

766 IC Sample Processor

2.4 Connection of devices to the remote interface

2.4.5 IC system with suppression with PC as "Master"

The 766 IC Sample Processor is connected to an IC system with suppression consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump and either 752 Pump Unit or 753 Suppressor Module as shown in Fig. 13 using the 6.2125.120 adaptor. With this interconnection, in which the PC is the "Master", the standard methods 'PC',

'PC Seg' and 'Preconc' can be used (see section 4.6).

732

733

709

766

6.2141.110 cable

6.2125.120 adaptor

Fig. 13: Interconnection with IC system with suppression with

PC as "Master"

2.4.6 IC system for simultaneous anion/cation determination

The 766 IC Sample Processor is connected to an IC system for simultaneous determination of anions and cations consisting of two 732 IC Detectors, the 733 IC Separation Center, two 709 IC Pumps and (if suppression is used) the 753 Suppressor Module as shown in Fig. 14 using the 6.2125.120 adaptor. With this interconnection the standard methods 'AnCat' and 'AnCatSeg' can be used (see section 4.6).

6.2125.090 cable

6.2125.060 cable

752

753

6.2143.210 cable

6.2143.200 cable

6.2141.110 cable

766

6.2125.090 cable

766 IC Sample Processor

732/1

733

709/1

6.2125.120 adaptor

6.2143.210 cable

732/2

6.2125.090 cable

709/2

6.2125.060 cable

753

Fig. 14: Interconnection for anion/cation system

6.2125.090 cable

6.2125.060 cable

6.2141.120 cable

2 Installation

2.4.7 IC system with sample dialysis

The 766 IC Sample Processor is connected to an IC system with sample dialysis consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump, 754 Dialysis Unit and (if suppression is used) the 753 Suppressor Module as shown in Fig. 15 using the 6.2125.120 adaptor. With this interconnection the standard method 'Dialysis' can be used (see section 4.6). If no suppression is used, the 754 Dialysis Unit can be connected to the 6.2125.120 adaptor using the 6.2143.200 cable.

732

733

709

766

6.2125.120 adaptor

6.2125.090 cable

6.2125.060 cable

754

753

6.2143.220 cable6.2141.110 cable

Fig. 15: Interconnection with IC system with dialysis

766 IC Sample Processor

2.5 Connection of devices to the RS232 interface

6.2125.060 cable

Printer cables, see section 2.5.2

2.5 Connection of devices to the RS232 interface

2.5.1 General information on RS232 interface

Many different instruments may be connected via the serial RS232 interface 3232. In addition to all Metrohm instruments that support the Metrohm remote control language (see section 5.2) any printer with serial interface (or parallel interface and parallel/serial converter) or a personal computer (PC) may be connected. Any other measuring instrument may be controlled via RS232 interface, as long as it supports serial data transmission.

766

732

Fig. 16: Connection possibilities for the RS232 interface

In order to guarantee safe data transmission, it is important to set the same RS232 interface parameters correctly for both instruments connected (see section 2.5.2).

Control commands (examples)

CTL:RS &M;$G starts a Metrohm instrument CTL:RS &M;$S stops a Metrohm instrument PRINT: config prints a configuration report to

a printer or PC

766 IC Sample Processor

Scanning input data (example):

SCN:RS : *R" waiting for readiness of a Metrohm

instrument

2 Installation

2.5.2 Connection of a printer

Printers with the following printer drivers may be connected:

IBM IBM Proprinter and printers with IBM emulation Epson Epson printers and printers with Epson emulation Seiko Seiko printers DPU-411 and DPU-414 Citizen Citizen printer IDP562-RS HP HP printers and compatibles with HP PCL3 emulation

If you connect other printers, ensure that these emulate a printer mode supported by the 766 IC Sample Processor.

Most printers with a serial interface are connected using the 6.2125.050 Cable. Printers with a parallel interface need a serial/parallel converter (e.g. 2.145.0300) and the 6.2125.020 Cable.

Before connecting a printer to the RS232 interface, switch off the 766 IC Sample Processor !

The interface parameters are set in the configuration submenu ">RS232

settings".

The following table provides information on a few selected printers.

Printer Cable RS232 settings Settings on printer

IBM Proprinter 6.2125.050

Seiko DPU-411

Seiko DPU-414

6.2125.020 baud rate: 9600

6.2125.130 baud rate: 9600

baud rate: 9600 data bit: 8 stop bit: 1 parity: none handshake: Hws character set: IBM

data bit: 8 stop bit: 1 parity: none handshake: Hws character set: Seiko

see printer manual

Settings of the DIP switches:

DIP01 DIP02

on off

1 2 3 4 5 6 7 8 1 2 3 4 5 6

The switchable 7-bit ASCII character set of the printer will be automatically set by the 732 IC Detector to the national character sets in accordance with the set dialog language.

Settings of the DIP switches:

Dip SW-1Dip SW-1 Dip SW-2Dip SW-2 Dip SW-3Dip SW-3 11 OFF ON ON 22 ON OFF ON 33 ON ON ON 44 OFF ON ON 55 ON ON OFF 66 OFF ON ON 77 ON OFF ON 88 ON OFF ON

The switchable 7-bit ASCII character set of the printer will be automatically set by the 732 IC Detector to the national character sets in accordance with the set dialog language.

766 IC Sample Processor

2.5 Connection of devices to the RS232 interface

Printer Cable RS232 settings Settings on printer

Citizen IDP562-RS

6.2125.050 baud rate: 9600

data bit: 8 stop bit: 1 parity: none handshake: Hws character set:Citizen

Epson with 6-pin round connector

6.2125.040 baud rate: 9600

data bit: 8 stop bit: 1 parity: none handshake: Hws character set: Epson

Epson with additional serial interface #8148

6.2125.050 baud rate: 9600

data bit: 8 stop bit: 1 parity: none handshake: Hws character set: Epson

Epson LX-300 6.2125.050 baud rate: 9600

data bit: 8 stop bit: 1 parity: none handshake: Hws character set: Epson

Epson and Canon printers with parallel interface

HP Deskjet with serial interface

HP Laserjet with serial interface

6.2125.020 +

2.145.0300 serial/ parallel converter

6.2125.050 or adaptor cable 25-pin neg./9-pin pos. (e.g. HP C2933A)

Adaptor cable 25-pin neg./9-pin pos. (e.g. HP C2933A)

baud rate: 9600 data bit: 8 stop bit: 1 parity: none handshake: Hws character set: Epson

baud rate: 9600 data bit: 8 stop bit: 1 parity: none handshake: Hws character set: HP

HP Deskjet/ Laserjet with parallel

6.2125.020 +

2.145.0300 serial/ parallel converter

baud rate: 9600 data bit: 8 stop bit: 1 parity: none handshake: Hws character set: HP

Settings of the DIP switches:

1 2 3 4 5 6 7 8 9

SSW1

The switchable 7-bit ASCII character set of the printer can be changed to the national character sets only by setting Dip switch 4 and 5:

44 55 Character setCharacter set

OFF OFF USA

ON ON Great Britain ON OFF France

OFF ON Germany

Spanish does not have its own character set (it is best to select French).

Settings of the DIP switches:

SW1 SW2

on off

1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8

Settings of the DIP switches on the interface:

SW1 SW2

on off

1 2 3 4 5 6 7 81 2 3 4 5 6

see printer manual

Settings of the DIP switches:

A B

on off

1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8

see printer manual

766 IC Sample Processor

2 Installation

766 IC Sample Processor

3 Operating tutorial

In order to become acquainted with the 766 IC Sample Processor and its mode of operation, it is helpful to work through the short Operating Tutorial. The basic operating steps that are required to prepare the first sample series and run it with a given method are described here.

For further explanations of the operation, please refer to section 4, which describes the functions of the individual keys and the programming in detail.

3.1 Prerequisites / Preparations

• It is assumed that the 766 IC Sample Processor is fully installed (see

section 2). Don’t forget to mount the splash protection and the plug cover.

• Connect a 732 IC Detector at the remote connection 2222 (see section

2.4).

• Choose a simple IC method that you have saved in the 732 IC De-

tector or create a new simple method.

• The <é> and <ê> keys can be used to move the lift up or down.

RESET

CLEAR

ENDSEQ

• Install the 6.2041.430 sample rack. Press <RESET> or

<ENDSEQ> + <ENTER>. The 766 IC Sample Processor is initialized in this way with the lift and rack placed in the rest position. In this position the magnetic rack code can be read so that the internally stored rack data (position table, etc.) can be loaded. This should be done after every rack change.

• Insert some sample tubes into the sample rack, beginning with

position 1. Using the keys <ç> and <è> the rack can be turned for this purpose.

766 IC Sample Processor

3 Operating tutorial

3.2 Configuration

• The dialog language can be set in the configuration menu. Press

CONFIG

ENTER

• and then <ENTER>.

configuration >auxiliaries

>auxiliaries dialog: english

TOWER

SELECT

4 × <ê>

<ENTER>

• This menu item has a colon, indicating that here the parameters can

be selected from a list. Press <SELECT> several times in order to view the various selections and get used to this type of dialog.

>auxiliaries dialog: deutsch

• With <ENTER> you can accept the suggestion 'dialog: eng-

lish'.

>auxiliaries display contrast: 3

• By pressing <ê> 4 times you reach the menu selection 'max. lift

way'.

>auxiliaries max. lift way 125 mm

• Here the lowest allowable lift position for automatic and manual

operation can be set. For the 6.2041.430 sample rack with

6.2743.050 sample tubes, this limit value of 125 mm should not be changed. Accept the value by pressing <ENTER>.

QUIT

STOP

configuration >rack definitions

• In order to put the 766 IC Sample Processor back into the initial

position, press <QUIT> or <STOP>.

******** counter 0/127 PUMP- ready

• In the normal state, the method name and the sample counter

reading are displayed in the first line. The second line serves as status line which displays the pump status and the changer status.

766 IC Sample Processor

3.3 Rack configuration

RESET

CLEAR

• At the end of this basic configuration the 766 IC Sample Processor

must be turned off and on again or re-initialized by pressing <RESET> to make the latest settings effective.

• All data entered up to this point however, are retained. The same is

true for any methods that may have been saved.

3.3 Rack configuration

• Using the keys <é> and <ê> you can run the lift to the desired

work position for the needle.

CONFIG

<ê>

<ENTER>

<ê>

RESET

CLEAR

• Now open the configuration menu with <CONFIG> and move the

cursor key <ê>, until you reach the submenu '>rack defini-

tions'. Press <ENTER> to open this submenu where you can de-

fine the rack configuration.

>rack definitions rack number 2

• The rack number of the engaged rack will be displayed as soon as

the sample rack has been correctly identified. By confirming with <ENTER> you access the rack data. (By entering another rack number you can also edit the data of a sample rack that is not engaged.)

• You can skip the first entries (code and rack type) with the cursor

key <ê>. Now you can enter the work position of the needle.

>rack definitions 2 work position 125 mm

• Because you have already positioned the needle at the desired

height, you can accept the current lift position directly by pressing <CLEAR>. Of course the work position can be entered manually or the value that has automatically been accepted can be modified later. Lift positions are given in millimeters (0…125 mm), measured from the uppermost limit (rest position) of the lift. Consider during the input of the working position that with sample tubes sealed with PE caps the work position must be set to 125 mm, since otherwise a vacuum can develop in the sample tube and the sample will not be aspirated correctly.

766 IC Sample Processor

3 Operating tutorial

>rack definitions 2 work position 71 mm

<ENTER>

• In any case don’t forget to confirm the value with <ENTER>.

>rack definitions 2 rinse position 125 mm

• The next menu item 'rinse position' defines the height at which

the lift must be when the needle is rinsed. As for the work position, the value here can also be entered manually or automatically accepted. For the latter, the configuration menu must be exited by pressing <QUIT> twice and the lift newly positioned.

>rack definitions 2 rinse position 105 mm

>rack definitions 2 shift position 0 mm

• The menu item 'shift position' defines the height of the needle

when the sample rack is rotated. For the 766 IC Sample Processor this height is set to 0 mm and cannot be changed.

• Press <ENTER>.

<ENTER>

STOP

>rack definitions 2 special position 0 mm

• The 'special position' defines a further height of the lift. For the

entry proceed as for the work position.

>rack definitions 2 special position 55 mm

• The final entry in the rack configuration is the definition of the posi-

tion of the special beakers.

>rack definitions 2 >>special positions

• In the submenu '>>special positions' enter the positions at which

you have placed conditioning or rinsing beakers (for the 6.2041.430 sample rack the two positions 'special beaker 1 128' and 'spe-

cial beaker 2 129' are already set).

• The configuration can now be exited with <STOP> or by pressing

<QUIT> three times. The rack data entered are now available at all times and must not be re-defined every time.

766 IC Sample Processor

3.4 Methods

USER

METHOD

<ENTER>

TOWER

SELECT

<ENTER>

• Now open the user method menu by pressing <USER METHOD>.

methods >recall method

• Press <ENTER> to load a predefined method.

>recall method method: ∗∗∗∗∗∗∗∗∗∗∗∗∗∗∗∗

• Choose 'SP' with the <SELECT> key. This is a universal method

with the 766 IC Sample Processor as "Master" from which you can learn the basic sample changer commands.

• After you have confirmed loading the method with <ENTER>, the

name of the method appears in the upper left corner of the display. You can now use the TRACE function to run the method in steps to understand how it works (see section 3.5).

3.5 "Tracing"

SAMPLE

• Before you begin tracing, set the position of the first sample with the

<2>

<ENTER>

PARAM

<3>

<ENTER>

• Press <2> and <ENTER>.

• Now press <PARAM> to open the parameter menu. All parameters

• The first menu entry defines the number of sample tubes (without

SAMPLE command. Press <SAMPLE>.

manual operation SAMPLE: = 1

and sequences that are stored with methods can be found here.

parameters number of samples: rack

the special beakers) that are to be treated in a series. Here you can choose between 'rack' (= a sample rack that is partially or completely filled, only positions with a sample tube are counted) and '*' (= infinite number of samples) with <SELECT>. However for this learning sequence, enter '3' on the keyboard. It is also possible here, as with the other parameters, to enter data manually or use the "select" choice.

766 IC Sample Processor

3 Operating tutorial

<ENTER>

<ê>

START

parameters >start sequence

• In the submenu '>start sequence' the commands that are exe-

cuted at the start of a sample series are found.

>start sequence 1 CTL:Rm: INIT

• The first command is a CTL command for the initialization of the

remote interface. This command should be used in the start sequence of every method. Do not change anything here and press <ê>.

>start sequence 2 CTL:Rm: PUMP 752 ON

• With the second CTL command the 752 Pump Unit used for the

operation of the suppressor is started. If you use a suppressor and the 752 Pump Unit is connected to the remote interface of the 766 IC Sample Processor, press <START>. The 752 Pump Unit is started.

<QUIT>

<ê>

<ENTER>

START

<START>

• Leave this submenu with <QUIT>.

• In the submenu '>sample sequence' you find the command se-

quences that are executed for every sample. It is recommended to test out this procedure line by line with the TRACE function.

>sample sequence 1 SCN:Rm : Pump1 ?

• In the first line the SCAN command is used to scan the status of the

709 IC Pump. The 766 IC Sample Processor 766 waits until the pump drive of the 709 IC Pump is switched on. Therefore switch on the pump drive at the 709 IC Pump. If you press <START> at this point, this command is executed and the next program line appears.

>sample sequence 2 MOVE 1 : sample

• Press <START> to place the sample tube in the position predefined

as sample position 2 below the needle.

>sample sequence 3 LIFT: 1 : work mm

<START>

LEARN

HOLD

• On the next line press <START> again to move the needle into the

work position you previously defined for this rack.

• With this command you can become acquainted with the LEARN

mode. It allows the user to manually set the parameters of a command on a trial basis.

• Press <LEARN> to access the LEARN mode. The blinking LEARN-

LED indicates that the 766 IC Sample Processor is ready to execute the command.

766 IC Sample Processor

<ENTER>

3.5 "Tracing"

• Now move the lift into the desired position with the <ê> and <é>

keys. You will notice that the current lift position is always indicated "live". During execution of the command the LEARN-LED is lit continuously. Accept the lift position that has been set by pressing <ENTER> and thereby exit the LEARN mode. The LEARN LED goes off again.

>sample sequence 4 CTL:Rm: FILL A 1

<START>

LEARN

HOLD

LEARN

HOLD

<ENTER>

• In this line the injection valve at the 733 IC Separation Center is

switched to the "Fill" position.

>sample sequence 5 PUMP 1.1 : 120 s

• In this line the peristaltic pump at the 766 IC Sample Processor is

started in order to convey the sample from the sample tube to the sample loop at the 733 IC Separation Center.

• Here, you can use the LEARN mode to optimize the pump time too.

• In this case, as with the other "teachable" commands (the LIFT

command is an exception), pressing the <LEARN> key causes immediate execution of the corresponding command. The elapsed time is also displayed here "live". By pressing the <LEARN> key again the command can be interrupted.

• The blinking LED indicates that the IC Sample Processor is still in the

LEARN mode. If you now switch the pump back on with the <LEARN> key, you will see that the "live" value (pump time) is now added to the existing value.

• Now set the pump time in this way. Accept the total time with

<ENTER> and exit the LEARN mode in this way.

<START>

<ENTER>

>sample sequence 6 CTL:Rm: ZERO 1

• In this line the autozero function at the 732 IC Detector is triggered.

>sample sequence 7 CTL:Rm: INJECT A 1

• In this line the injection valve at the 733 IC Separation Center is

switched to the "Inject" position.

>sample sequence 8 WAIT 1200 s

• In this line a waiting time is defined that is used for the acquisition of

the chromatogram.

• The LEARN mode can also be used with the WAIT command.

766 IC Sample Processor

3 Operating tutorial

>sample sequence 9 NOP

<QUIT>

<ê>

<ENTER>

START

• An empty line with a 'NOP'-entry (no operation) always forms the end

of a sequence.

• Exit the sample sequence with <QUIT> and go to the final se-

quence.

• After all sample tubes have been processed, the final sequence is

executed.

>final sequence 1 CTL:Rm: PUMP R/S 1

• In this line the pump drive at the 709 IC Pump is switched off. Press

<START>.

>final sequence 2 CTL:Rm: PUMP 752 OFF

• In this line the pump drive at the 752 Pump Unit is switched off.

Press <START>.

>final sequence 3 NOP

PARAM

QUIT

START

SAMPLE

• Now you have reached the end of the final sequence and have

completed the entire run of a sample series.

• By pressing <QUIT> twice the 766 IC Sample Processor returns to

the normal state.

• Now prepare some sample tubes and place them on the sample

rack. Prepare the IC system with 732 IC Detector, 733 IC Separation Center, 709 IC Pump and 752 Pump Unit for the recording of chromatograms.

• Enter the number of samples to be processed (<PARAM>) and

define the position of the first sample (SAMPLE = 1).

• Now you can start your first sample series with <START>.

766 IC Sample Processor

4 Operation

This section provides a detailed description of the operation of the 766 IC Sample Processor using the keyboard and dialog display. The overview of the fundamentals of operation and key functions (section 4.1) is followed by a detailed description of configuration settings (section 4.2), methods (section 4.3), manual operation (section 4.4), sample racks (section 4.5), and standard methods (section 4.6).

4.1 Fundamentals of operation

4.1.1 Display

The display on the 6.2142.010 keyboard consists of two lines, each having 24 characters.

The first line serves as a title line in which the current method and the sample counter reading are displayed. In the edit mode the menu title is shown.

The second line serves as a status line which displays specific activities depending on the operating state. In the edit mode it serves as an entry line.

Normal State

Method name èè

Pump status èè

Method processing

******** counter 1/127 PUMP- ready

Sample counter êê

çç Changer status

766 IC Sample Processor

Method name èè

Running sequence èè

Edit mode

Menu title èè

Menu line/command èè

******** counter 2/127 START 03 WAIT 11 s

éé Current command with line number

>sample sequence 1 MOVE 1 : sample

é 1st Parameter

çç Parameter

çç 2nd Parameter

4 Operation

4.1.2 Keyboard

SC Controller

******** counter 1/999 PUMP- ready

TOWER 1 TOWER 2 LEARN

CONFIG

HOME

PARAM

USER

METHOD

END

SAMPLE

PUMP

MOVE

STIR DOS

4 5

TOWER

INSERT

DELETE QUIT ENTER

SELECT

RESET

CLEAR

SCAN

DEF

LEARN

CTRL

MetrohmMetrohm

LIFT

WAIT

ENDSEQ

HOLD

6.2142.010

STARTSTOP

Most of the keys have two functions according to whether the 766 IC Sample Processor is in the normal state or in the edit mode.

The uppermost row contains the keys which make the menus accessible (<CONFIG>, <PARAM>, <USER METHOD>). Here with the help of the keys on the left side of the keyboard, you can navigate and change parameters. For the latter, the numerical keypad on the right half of the keyboard is available. Except for the menu 'methods' the entries under these selection menus can be altered while a method is in process and for the most part, have an immediate effect on the procedure which is running.

The lowermost row of keys (<HOLD>, <STOP>, <START>) is used for the direct control of method processing.

766 IC Sample Processor

4.1.3 Overview of key functions

Key Normal state Edit mode

4.1 Fundamentals of operation

CONFIG

PARAM

Open the configuration menu

• The <CONFIG> key opens the

selection menu for the configuration of the 766 IC Sample Processor.

• The settings in the configuration

menu remain constant until they are changed or the working memory (RAM) for the configuration settings is re-initialized.

Open the parameter menu

• The <PARAM> key opens the

selection menu for the changer settings.

• All settings that are set in the

parameter menu belong to a method and will be saved with the method. These parameters are method-specific.

Select configuration settings

• When the configuration menu is

open, pressing the <CONFIG> key displays the next menu line.

• After the last line is displayed, the

first one follows.

• <QUIT> exits the menu.

Select method parameters

• When the parameter menu is open,

pressing the <PARAM> key displays the next menu line.

• After the last line is displayed, the

first one follows.

• <QUIT> exits the menu.

USER

METHOD

HOME

Open the user method menu

• The <USER METHOD> key opens

the selection menu for the loading, saving and deletion of user-defined methods.

Bring lift to zero-position

• The <HOME> key runs the lift with

the needle to the zero-position (0 mm), i.e. to the upper stop.

Select method functions

• When the user method menu is

open, pressing the <USER METHOD> key displays the next menu line.

• After the last line is displayed, the

first one follows.

• <QUIT> exits the menu.

Select the first line of a menu

• With the <HOME> key, the first

line in a menu or a sequence can be accessed.

• Any data that has been altered in a

menu or command line is not carried over (see <ENTER> key).

766 IC Sample Processor

4 Operation

Key Normal state Edit mode

END

Lift in work position

• The <END> key runs the lift into

the work position.

• The work position is defined

separately for every sample rack in the configuration menu under

'>rack definitions' (in mm from

the rest position, i.e. as measured from the upper stop).

Run lift upwards

• Run lift upwards. The lift movement

is executed as long as the key remains pressed.

Select the last line of a menu

• With the <END> key, the last line

in a menu or a sequence can be accessed.

• Any data that has been altered in a

menu or command line is not carried over (see <ENTER> key).

Select previous menu line

• In a Select menu or a sequence the

<é> key accesses the previous line.

• Any data that has been altered in a

menu or command line is not carried over (see <ENTER> key).

Run lift downwards

• Run lift downwards. The lift

movement is executed as long as the key remains pressed.

Turn rack left

• The <ç> key turns the sample

rack one position to the left, i.e. in the counterclockwise direction. The next highest beaker position is placed under the needle.

• The turning speed of the rack can

be defined in the Parameter menu.

• The rotation of the rack can only be

carried out when the lift is at or above the shift position.

Select next menu line

• In a Select Menu or a sequence the

<ê> key accesses the next line.

• Any data that has been altered in a

menu or command line is not carried over (see <ENTER> key).

Move the cursor one column to the left

• With the <ç> key the cursor is

moved one column to the left in an edit line with two parameters.

• Any altered data will not be carried

over during this action (see <ENTER> key).

766 IC Sample Processor

Key Normal state Edit mode

4.1 Fundamentals of operation

INSERT

Turn rack right

• The <è> key turns the sample

rack one position to the right, i.e. in the clockwise direction. The next lowest beaker position is placed under the needle.

• The turning speed of the rack can

be defined in the Parameter menu.

• The rotation of the rack can only be

carried out when the lift is at or above the shift position.

Move the cursor one column to the right

• With the <è> key the cursor is

moved one column to the right in an editing line with two parameters.

• Any data which has been altered

will not be carried over during this action (see <ENTER> key).

Add a command line to a sequence

• Adds a new command line above

the current line in a sequence.

The NOP command (no operation) automatically occupies this line and has no function.

• The lines following this line are

shifted one line downwards.

DELETE

TOWER

SELECT

Delete a command line in a sequence

• Deletes the current line in a

sequence.

• The lines which follow shift upwards

by one line.

Select parameter

• With the <SELECT> key given

data values can be selected for a particular parameter in manual operation.

• With every repeated keystroke the

next value that can be selected is displayed. The last value is followed again by the first.

• The data is accepted with

<ENTER>.

766 IC Sample Processor

4 Operation

Key Normal state Edit mode

RESET

CLEAR

QUIT

Initialization of the IC Sample Processor

• The <RESET> key serves to

initialize the IC Sample Processor.

• A method in the working memory

remains unchanged. The sample rack and the lift return to their initial positions in this case.

Interruption of a method after the current sequence

• During processing of a method, the

sample series can be aborted with <CLEAR> so that the sample currently being processed is processed to completion. The final sequence is not executed in this case.

Aborting a command already in operation

• When a sequence is being

processed the currently running command will be aborted and continued in the course of the next command line. This is useful when a programmed waiting time should be shortened or when a signal cannot be recorded with a SCAN command.

Quitting error message

• With the <QUIT> key error

messages can be acknowledged. Before acknowledging error messages, the cause should be remedied.

• The command during which the

error message occurs will nevertheless be carried out (during manual operation).

• If an error occurs during method

processing the error message is acknowledged by pressing the <QUIT> key and the method is interrupted (HOLD status). Afterwards <START> can be used to continue with the following command line or <STOP> will halt processing.

Deleting parameters, setting the default values

• The <CLEAR> key sets the initial

(default) value given for a parameter.

Delete last character

• In text edit mode the last character

will be deleted with <CLEAR> (Backspace).

Abort entry, select next highest menu level

• With the <QUIT> key the active

(sub)menu or a menu or command line is exited. The next highest menu level or the basic state is selected.

• Any data from a menu or command

line which has been changed will not be taken over in this case. This will be signaled by an acoustic signal (see <ENTER> key).

• <QUIT> acknowledges error

messages.

766 IC Sample Processor

Key Normal state Edit mode

Accept data, next line

ENTER

• The <ENTER> key accepts the

value entered and selects the next menu line.

• A modification of data or parame-

ters must always be confirmed by <ENTER>, otherwise the change will not be accepted.

• If a change in a parameter is not

confirmed by <ENTER> and another menu line is accessed, the previous value will be retained. This is indicated by an acoustic signal.

4.1 Fundamentals of operation

SAMPLE

MOVE

Set sample position

• The <SAMPLE> key serves to set

the current sample position.

• When starting a method, this

position is assumed to have the first sample of a series.

• If the current sample position is not

manually set before the start of a sample series, rack position 1 is always started first.

Position sample tube

• Turn the sample rack to position

the described sample tube under the needle. In addition to the predefined sample tubes, the eight rack-specific special beakers available can be placed. Absolute positions can also be chosen.

• The turning speed can be altered in

the Parameter menu.

Numerical entry ('7')

Set sample position

• In a start sequence the SAMPLE

command serves to define the first sample of a sample series.

• If no SAMPLE definition is made in

any sequence, the manually set rack position is assumed to be the first sample.

Numerical entry ('8')

Position sample tube

• Turning the sample rack to position

the described sample tube under the needle. In addition to the predefined sample tubes, the 8 rack specific special beakers available can also be placed. Absolute positions can also be chosen.

• The turning speed can be altered in

the Parameter menu.

766 IC Sample Processor

4 Operation

Key Normal state Edit mode

LIFT

PUMP

Positioning the lift

• Raises or lowers the lift to a

predefined position. These positions (work position, rinse position, shift position, special position) can be defined in the Configuration menu as rack-specific.

• An absolute lift position in mm can

also be given.

Control pump

• The <PUMP> key is used to

switch the peristaltic pump of the 766 IC Sample Processor on or off.

• The state of the pump is shown in

the second display line in the normal state. 'PUMP+' means "switched on", 'PUMP–' means "switched off").

Numerical entry ('9')

Positioning the lift

• The lift can be run to the predefined

positions (work position, rinse position, shift position, special position) in a sequence.

• An absolute lift position in mm can

also be given.

Numerical entry ('4')

Control pump

• The pump can be specifically

switched on and off in a sequence or set to operate for a fixed amount of time (in seconds).

SCAN

Display input signals

• Display of the incoming signal or

data on the remote or RS interface. This function serves to control the data communication or states of connected instruments.

• The first parameter represents the

interface selection. The signals for data being received at that moment are displayed as the second parameter.

• If the parallel remote interface (Rm)

is selected, the signal states of the incoming remote lines are displayed in binary form (1 = line active, 0 = line inactive). Details see section 5.1.

• If the serial RS232 interface (RS) is

selected, the character string being received is displayed line by line (14 characters). Details see section

5.2.

Numerical entry ('1')

Scan input signals

• In a sequence the SCAN command

causes method processing to stop until the predefined bit pattern (with the remote interface) or the given character string (with the RS232 interface) is received.

• Predefined bit patterns are

available for the remote interface and can be selected via simple short-names (e.g. 'Ready 1' or

'End 2').

• Character strings consisting of 14

ASCII characters may be defined with the RS232 interface.

766 IC Sample Processor

Key Normal state Edit mode

4.1 Fundamentals of operation

CTRL

Interface control

• Controlling of external instruments

via the remote and RS232 interface. This function is used for data communication with or control of connected instruments.

• The first parameter stands for the

selection of the interface. The second parameter defines the state of the remote output lines or data to be transmitted via RS232 interface.

Parameters for remote interface

• Bit pattern with 14 digits (0, 1 or *)

for the 14 output lines or predefined patterns accessible by the <SELECT> key ('INIT 732',

'FILL A 1', etc.).

Parameters for RS232 interface

• Character string with up to 14

alphanumerical characters. Default value: '&D.S"9"', may be set with <CLEAR>. Most Metrohm instruments can be controlled with such remote control commands, see section 5.2.

Numerical entry ('2')

Interface control

• Setting the 14 signal lines of the

remote interface or sending a character string via the RS232 interface to control instruments connected.

• Predefined bit patterns are

available for the remote interface and can be selected via simple short-names (e.g. 'INIT 732' or

'FILL A 1').

• Character strings consisting of 14

ASCII characters may be defined with the RS232 interface.

WAIT

DEF

Print report

• The <PRINT> key serves for the

manual printout of reports.

• The choice of printer type and the

parameters of the RS232 interface must be done in the Configuration menu under '>RS232 settings'.

Numerical entry ('3')

Define waiting time

• Waiting for a certain time interval to

elapse in a sequence.

Numerical entry ('0')

Numerical entry ('.')

Text entry

• In a menu or sequence line in

which entry of text is required, (e.g. method name), text edit mode is activated with "<".

• Existing text is deleted in this event

and the text cursor is set on the left margin of the text field.

766 IC Sample Processor

4 Operation

Key Normal state Edit mode

• "<" serves also for shifting the

chain of characters to chose from, i.e. the text cursor is shifted one place to the left for each instance (see section 4.1.6).

ENDSEQ

Initialization

• The 766 IC Sample Processor is

initialized with the <ENDSEQ> key followed by <ENTER>.

• A method in the working memory

remains unchanged. The rack and the lift are run to the initial position.

Text entry

• In a menu or sequence line in

which entry of text is required, (e.g. method name), text edit mode is activated with ">". Existing text remains in this event and the text cursor is set at the end of the existing character string.

• ">" serves also for shifting the

chain of characters to chose from, i.e. the text cursor is shifted one place to the right for each instance (see section 4.1.6).

Set end mark

• For test purposes an <ENDSEQ>

command can be placed at any line desired in a sequence. This has the effect that the sequence is only executed up to this end mark.

START

Start a method

• The <START> key starts a

method. Starting is only possible when the IC Sample Processor is in the normal state, i.e. when 'ready' is shown in the display.

• When starting a sample series, the

sample counter is set to 0.

• When <START> is pressed after

an interruption (<HOLD>), the sequence is continued with the next command line.

Trace function

• During editing of a sequence, the

command defined in the command line can be directly executed with the <START> key.

• A sequence can therefore be tested

from start to finish (or in parts) in single steps ("tracing").

766 IC Sample Processor

Key Normal state Edit mode

4.1 Fundamentals of operation

STOP

Stop process and peripheral instruments

• The <STOP> key terminates a

method.

• Any peripheral instruments that are

connected (e.g. 709 IC Pump) are not automatically stopped. In

'>manual stop options', a sub-

menu of the Parameter menu, you can specify what signal or data is to be transmitted via the interface involved (remote or RS232) during manual activation of the <STOP> key. The connected instrument can be halted or if necessary, initialized (see section 4.3.2).

• During a manual halt of a sample

series with <STOP>, the end sequence of the method will not be executed.

• In the normal state the <STOP>

key also stops the pump. The

'>manual stop options' for con-

nected peripheral instruments are also effective in the normal state.

Stop editing

• <STOP> causes the editing to

abort and the instrument to return to the normal state. (exception: Process sequences).

LEARN

HOLD

Interrupt Process

• The <HOLD> key interrupts the

processing of a method. However connected peripheral instruments (e.g. 709 IC Pump) are not halted. Only method processing is interrupted. In the "HOLD" state a method can be completely halted with <STOP> or continued with the next command in line by pressing <START>.

• After quitting an error message

during method processing the IC Sample Processor automatically goes into the "HOLD" state.

Switch on LEARN mode

• The <LEARN> key serves to start

the LEARN mode. This mode is provided for easier editing of process sequences. It allows direct acceptance of a parameter value that has been set by manual control. The LEARN mode is available for the following commands:

LIFT, PUMP, SCN, WAIT

• Details concerning the LEARN mode see section 4.3.4.

766 IC Sample Processor

4 Operation

4.1.4 Instrument dialog

The instrument dialog of the 766 IC Sample Processor is organized in the form of so-called rolling inquiries which are arranged in menu levels in a hierarchical manner and are subject to the following rules:

Main menu

The main function keys <CONFIG>, <PARAM> and <USER METHOD> of the 766 IC Sample Processor open a main menu whose thematically arranged submenus are selected by repeated pressing of this key or with <ê>. The name of the key always appears in the top line of the display.

Submenu

Each submenu has a title marked by ">" which appears in the bottom line of the display. <ENTER> is used to move from the title to the individual inquiries, which are used to change the most important settings of the instrument. The top line of the display always shows the title of the active submenu.

Navigating within the menus is accomplished with the cursor keys; <HOME> jumps to the first, <END> to the last menu line.

<QUIT> exits the active menu, opens the next highest menu level or returns to the normal state.

<ENTER> either opens a submenu or confirms data entry at the lowermost menu level.

Inquiries

In inquiries without ":", the values must be entered using the numeric keys. <ENTER> is used to accept the inputted value and the next inquiry appears.

In the case of inquiries with ":", the admissible values can be selected with the <SELECT> key>. <ENTER> is used to accept the set value and the next inquiry appears.

Depending on the parameter, <CLEAR> is used to reset the displayed value to the smallest possible value or the default value. The <CLEAR> key also serves to abort wrong entries.

<QUIT> is used to quit the inquiries and return to the main menu. You will find a schematic representation of the instrument dialog in

Fig. 17.

766 IC Sample Processor

Normal state

KEY

4.1 Fundamentals of operation

QUIT

Kex title >Submenu 1

KEY

Key title >Submenu 2

KEY

ENTER

>Submenu 1 Inquiry 1

ENTER

>Submenu 1 Inquiry 2

>Submenu 1 Inquiry n

>Submenu 2 Inquiry 1

ENTER

QUIT

>Submenu 2 Inquiry 2

ENTER

>Submenu 2 Inquiry n

Key title >Submenu n

KEY

Fig. 17: Schematic representation of the instrument dialog

766 IC Sample Processor

4 Operation

4.1.5 Data entry

Edit line

In a menu line or a sequence one or two parameters respectively can be entered. A blinking block cursor indicates where a parameter can be entered.

>changer settings lift rate 1 ÛÛ12 mm/s

é é é

ç title line ç edit line

menu item cursor parameter

>sample sequence 7 PUMP 1.1 : ÛÛ150 s

é é é

ç title line ç edit line

command 1st param. 2nd param.

The cursor keys<è> and <ç> can be used to switch between the parameters. Pressing <ENTER> shifts the cursor automatically to the right, pressing <QUIT> correspondingly to the left.

<Select> choices (Roll-up selection)

TOWER

SELECT

Data can usually be entered directly via the numerical keypad block on the keyboard. Pressing <SELECT> at entries which are specially identified by a colon displays a preset selection of data. This selection is cyclic, structured like a revolving drum.

Example:

>RS232 settings character set: IBM

>RS232 settings character set: Epson

Epson Seiko

Citizen

IBM

<ENTER>

766 IC Sample Processor

4.1.6 Text entry

The text editor can be used when text entry is provided. Numbers can be entered directly via the keyboard.

Opening the text editor

ENDSEQ

The keys "<<" or ">>" open the text editor. With "<<" an existing character

string is deleted and the text cursor is set to the left margin of the edit line. With ">>" an existing character string remains and the text cursor is set on the last character of the existing text.

A character chain is displayed that is composed of all the characters in alphabetical order that can be entered. The blinking character is the currently selected one (text cursor).

Character selection

The keys "<<" and ">>" move the character chain composed of all

possible characters (capital and small letters, numbers and special characters, in alphabetical order) in the desired direction underneath the text cursor. Pressing these keys once has the effect that the

character chain is shifted one position left or right. The character chain can be shifted quickly by pressing the keys longer.

4.1 Fundamentals of operation

ENTER

RESET

CLEAR

QUIT

Confirmation of the character selection

The <ENTER> key causes the character that is currently positioned at the text cursor to be appended to the existing text. When the entire width of the edit line is filled, text edit mode is left and the text is accepted with <ENTER>.

Delete character

The <CLEAR> key deletes the last character of the existing text line. The text cursor automatically shifts one character to the left.

Exit text entry

With <QUIT> the text edit mode is exited. The text line displayed can be accepted with <ENTER> or rejected by pressing <QUIT> a second time.

766 IC Sample Processor

4 Operation

Scheme

>store method method: ********

"<" or ">"

blinking text cursor

>store method method:

<ENTER>

>store method method:

>store method method: Chloride

ÛÛ

ABCDEFGHIJ...abcd...1234...*%&/()=...

ÛÛ

DEFGHIJ...abcd...1234...*%&/()=...

<QUIT>

<ENTER>

blinking text cursor

"<" or ">"

...<ENTER>...

<QUIT>

character chain

2x ">"

reject

An entire text line can be entered in this way, for example, for the naming of a method. Text editing can be ended with <QUIT>. The text line will be displayed in its entirety and can be accepted with <ENTER> or rejected with <QUIT>.

766 IC Sample Processor

4.2 Basic settings

4.2.1 Configuration – <CONFIG> key

The <CONFIG> is used for the entry of basic settings of general appli-

CONFIG

cability. If you have changed the configuration, press <RESET> or switch on and off the instrument to make the entries effective. The <CONFIG> key opens the following main menu:

4.2 Basic settings

configuration >auxiliaries

configuration >rack definitions

configuration >RS232 settings

configuration >auxiliaries

Various general instrument settings

Sample rack settings

Settings for RS232 interface

Repeated pressing of the <CONFIG> key selects the submenus in turn. Use <ê> or <é> to move up or down one menu item; use <HOME> or <END> to move to the first or last menu item respectively. The submenu is opened with <ENTER>, <QUIT> returns to the normal state. The following listings show all dialog items which appear under <CONFIG>. The values shown in the display are the default values, the possible entry values or ranges are shown below the display.

General instrument settings

>auxiliaries dialog: english

english,deutsch, français,español

>auxiliaries display contrast 3

766 IC Sample Processor

0…7

Dialog language

english English

deutsch German français French español Spanish

Display contrast

0 = large contrast 7 = weak contrast

4 Operation

>auxiliaries beeper: ON

ON,OFF

>auxiliaries device label

8 ASCII characters

>auxiliaries program 5.766.0010

read only

>auxiliaries max. lift way 125 mm

0…125 mm

Acoustic warning signal

ON Acoustic warning signal in case of error

messages

OFF No warning signal

Device label

Freely selectable ASCII character string for characterizing the instrument (text entry, see section 4.1.6).

Number of the program version

Display only (no entry possibility). Please specify this number in inquiries to Metrohm.

Maximum stroke path for needle

The following entry becomes effective after a RESET or switching the IC Sample Processor off and on again.

This setting for max. lift way is important for safe operation. If the value for this entry is correct, damage to the needle can be avoided because this prevents the needle from being driven lower than the position indicated. If you use the 6.2041.430 sample rack with

6.2743.050 sample tubes, the default value of 125 mm should be retained.

Pressing <CLEAR> directly accepts the current lift position of the active tower.

configuration >rack definitions

>rack definitions rack number 2

>rack definitions 2 code 010001

Definition of sample racks

Number of the rack

The number of the rack in position is automatically dis-

1…16

played here, when its configuration is already stored and when a RESET has been performed. If the configuration of another rack must be changed, its rack number must be entered and confirmed with <ENTER>. The rack number will be shown in the first menu line for the subsequent entries. For more information concerning sample racks, see section 4.5. The number 2 is assigned to the 6.2041.430 sample rack delivered.

Identification code of the rack

The rack code must be unique and can only occur once

6 bits

in the instrument (see section 4.5).

766 IC Sample Processor

4.2 Basic settings

>rack definitions 2 type: M129-2

M129-2……

>rack definitions 2 work position 125 mm

0…125 mm

If you use the steel needle for 6.2743.050 sample tubes which are sealed with 6.2743.060 PE caps, the working position must always be set to 125 mm, since otherwise a vacuum can develop in the sample tube and the sample will not be aspirated correctly.

>rack definitions 2 rinse position 125 mm

0…125 mm

Type description of the rack

<SELECT> enables the choice of Metrohm-specific and self-defined rack types (see section 4.5).

Working position of the needle

in mm from the upper stop. Pressing <CLEAR> directly accepts the current lift po-

sition.

Rinsing position of the needle

in mm from the upper stop. Pressing <CLEAR> directly accepts the current lift po-

sition.

>rack definitions 2 shift position 0 mm

0 mm

>rack definitions 2 special position 0 mm

0…125 mm

>rack definitions 2 >>special positions

Submenu

>>special positions special beaker 1 128

0…number of positions

Shifting position of the needle

in mm from the upper stop. Only the value '0' is permissible.

Special position of the needle

in mm from the upper stop. Pressing <CLEAR> directly accepts the current lift po-

sition.

Special positions submenu

Position of special beaker 1

>>special positions special beaker 2 129

0…number of positions

etc. up to special beaker 8

766 IC Sample Processor

Position of special beaker 2

Up to 8 special beaker positions can be defined (see section 4.5).

4 Operation

configuration >RS232 settings

>RS232 settings baud rate: 9600

9600,4800,2400,

1200,600,300

>RS232 settings data bit: 8

>RS232 settings stop bit: 1

>RS232 settings parity: none

none,odd,even

Settings for RS232 interface

For further details on the RS232 interface, see section 5.2.

Data transmission rate (baud rate)

Data transmission rate in bit/s

Data bits

7,8

Stop bits

1,2

Parity

none Parity is not checked. odd Odd parity. even Even parity.

>RS232 settings handshake: HWs

HWs,HWf,

SWchar,SWline,none

>RS232 settings character set: IBM

IBM,HP,Epson, Seiko,Citizen

>RS232 settings RS control: ON

ON,OFF

Handshake

HWs Reduced hardware handshake. HWf Full hardware handshake. SWchar Software handshake with character stop. SWline Software handshake with line stop. none No handshake.

For detailed information on the handshake, see section

5.2.8.

Character set for printer or PC

The settings for the printers recommended by Metrohm are listed in section 2.5.2. For printers not listed, the setting 'Epson' is recommended. In any case the printer handbook should be consulted. For data transfer with personal computers, 'IBM' must be chosen.

Control via RS232 interface

ON Data receipt via RS232 interface switched

on.

OFF Data receipt via RS232 interface switched off

(no external control via RS232 possible).

766 IC Sample Processor

4.2.2 Locking keyboard functions

4.2 Basic settings

Switch on +

CONFIG

Certain domains of the user dialog can be made inaccessible to the novice user by locking particular keys. For example, the inadvertent overwriting of a method or even the changing of parameters can be prevented in this way.

The menu '>keyboard options' for the corresponding functions is opened by holding down the <CONFIG> key while switching on the 766 IC Sample Processor. Alternatively, a reset can be executed by pressing <CLEAR> and then the <CONFIG> key within

0.4 seconds. This menu is also accessible when the entire keyboard has been locked

The individual key domains that can be locked are the following:

>keyboard options lock keyboard: OFF

ON,OFF

>keyboard options lock configuration: OFF

ON,OFF

Locking the entire keyboard

If, during routine use, only one particular method is to be used, it may be desirable to block manual manipulations on the 766 IC Sample Processor. Almost all the keys on the keyboard can be locked for this purpose. The <START>, <STOP> and <CLEAR/RESET> keys however, remain operable so that it is still possible to start and stop a method. This can also be beneficial when using the 766 IC Sample Processor with a PC software (e.g. «IC Metrodata»). For this application the keyboard may be disconnected.

Locking <CONFIG> key

The basic configuration of the changer can be protected from overwriting. All settings of the configuration menu are no longer accessible at this point.

>keyboard options lock parameters: OFF

766 IC Sample Processor

ON,OFF

Locking <PARAM> key

If user methods are generally used, it might be wise to protect the stored method parameters from alteration. The parameter menu can then be made inaccessible.

4 Operation

>keyboard options >user methods

>user methods lock method recall: OFF

>user methods lock method store: OFF

>user methods lock method delete: OFF

Submenu for locking method storage functions

Lock loading of methods

ON,OFF

Lock storage of methods

ON,OFF

Lock deletion of methods

ON,OFF

>keyboard options lock display: OFF

ON,OFF

Locking the display

If the IC Sample Processor is to be operated exclusively by an external control software (e.g. «IC Metrodata»), the display for manual operation can be switched off.

766 IC Sample Processor

4.3 Methods

4.3.1 Structure of a method

A method consists of the following parts:

• Number of samples to be processed

• Process sequences (start, sample and final sequence)

• Definition of the various instrument settings

(changer settings, manual stop options)

These method parameters are accessible by pressing the <PARAM> key (see section 4.3.2). With the <USER METHOD> key, methods can be stored, loaded and deleted (see section 4.3.8).

A sequence is a succession of commands that are carried out in the order specified during automatic processing of a sample series. There are functions for controlling lift and pump and for moving the turntable (racks). External instruments (732 IC Detector, 709 IC Pump, 752 Pump Unit, etc.) can be controlled using the corresponding commands.

4.3 Methods

Processing a sample series is accomplished in three phases. These are:

• Start sequence Sequence of commands that is exe-

cuted once at the beginning of a series.

• Sample sequence Sequence of commands used for each

sample.

• Final sequence Sequence of commands that is exe-

cuted once at the end of a series.

number of samples

<START>

start sequence final sequence

sample sequence

normal state

The creation of sequences is done in the submenus '>start se-

quence', '>sample sequence' and '>final sequence', which are ac-

cessible via the parameter menu (see section 4.3.2). A sequence is organized in lines. When a command is entered, a new

line with the corresponding command is added after the command that is displayed at the moment. The line number is visible in the display. 99 lines per sequence are possible.

766 IC Sample Processor

In a command sequence, the commands that are on the numerical keypad as alternate functions can be used. For the most part these are the same commands that are used for manual operation (see sec- tion 4.4). However, in a sequence these can exhibit different or more extensive selection possibilities.

4 Operation

4.3.2 Method parameters – <PARAM> key

The <PARAM> key combines the most important parameter settings

PARAM

for the 766 IC Sample Processor. All these settings constitute a method and may be saved as such (see section 4.3.8). The key opens the following main menu:

parameters number of samples: rack

parameters >start sequence

parameters >sample sequence

parameters >final sequence

parameters >changer settings

parameters >manual stop

Number of samples to be processed (parameter)

Start sequence before sample series

Sample sequence for sample series

Final sequence after sample series

Sample changer settings

Options with manual stop

Repeated pressing of the <PARAM> key selects the submenus in turn. Use <ê> or <é> to move up or down one menu item; use <HOME> or <END> to move to the first or last menu item respectively. The submenu is opened with <ENTER>, <QUIT> returns to the normal state. The following listings show all dialog items which appear under <PARAM>. The values shown in the display are the default values, the possible entry values or ranges are shown below the display.

parameters number of samples: rack

1…999,rack,*

Number of samples to be processed

rack = all sample positions of the rack * = infinite number of samples

All sample positions of an engaged rack will be processed when the instrument is on the 'rack' setting (max. number of rack positions – number of special beakers defined). It is important that the 766 IC Sample Processor can recognize the rack. This is only possible when the rack is at the ground position. It is recommended to initialize the 766 IC Sample Processor with the <CLEAR> key or <ENDSEQ> and <ENTER> after every rack change.

766 IC Sample Processor

4.3 Methods

parameters >start sequence

parameters >sample sequence

parameters >final sequence

Start sequence of a sample series

The processing sequence entered here is executed once at the start of a sample series. This can be useful for switching

on external devices, for example. In this submenu up to 99 command lines can be entered as a

processing sequence (see section 4.3.3).

Processing sequence for each sample

This processing sequence is executed during the processing of every sample of a series.

In this submenu up to 99 command lines can be entered as a processing sequence (see section 4.3.3).

Final sequence of a sample series

This processing sequence is executed once at the end of a sample series. This could be the useful for switching off external devices, for example.

In this submenu up to 99 command lines can be entered as a processing sequence (see section 4.3.3).

parameters >changer settings

>changer settings rack number 2

>changer settings lift rate 1 12 mm/s

>changer settings shift rate 20

0…16

3…12 mm/s

3…20

Settings for 766 IC Sample Processor

Rack that is assigned to the method

0 = no particular rack

This setting can force the use of a certain rack with the method chosen. If this is not desired, the rack number

'0' must be chosen.

Stroke speed of lift

Rack shift rate

Turning speed of the rack in angular degrees/second

766 IC Sample Processor

4 Operation

parameters >manual stop

>manual stop CTL Rmt: **************

14 bit (1,0 or *)

>manual stop CTL RS232:

14 ASCII characters

Actions at manual stop

The following entries define the commands or signals that are transmitted via the interfaces when the <STOP> key is pressed. This enables peripheral instruments connected to be stopped automatically.

Signal output via remote interface

The 3 lines 11, 12 and 13 are occupied for the swing head and are therefore ignored (see section 5.1).

Data to be transmitted via RS232 interface

Clear value '&PR;$S'

4.3.3 Programming of sequences

The creation of sequences is done in the submenus '>start se-

quence', '>sample sequence' and '>final sequence', which are ac-

cessible via the parameter menu (see section 4.3.2). Each sequence is organized in lines. In each line, the commands that

are on the numerical keypad as alternate functions can be used to enter commands (see section 4.3.6 ). After selecting a command and entering the necessary data, the entry is accepted with <ENTER>. The line number is visible in the display. 99 lines per sequence are possible.

Navigation in a sequence is accomplished as in the other menus. In addition the <INSERT> and <DELETE> keys can be used.

<INSERT> adds a new command line above the current line in a

INSERT

DELETE

sequence. It is automatically occupied by the "NOP" command that has no function. The following lines are shifted one line downwards.

<DELETE> deletes the current line in a sequence. The following lines are shifted one line upwards.

The "LEARN" mode is available for the easy entry of parameters (details see section 4.3.4).

Furthermore the "TRACE" function can be used to execute every command line step by step (details see section 4.3.5).

766 IC Sample Processor

4.3.4 LEARN mode

4.3 Methods

LEARN

HOLD

When editing a method, the parameters of a command are most easily determined experimentally, i.e. by manual execution, and it is for this reason that certain commands are "teachable". The LEARN function makes the manual execution of particular changer commands possible during the editing of a sequence. The resulting parameters (for example, the lift position or the status of the input lines) can be taken over in the current command line. The LEARN function can be used repetitively. When times or volumes are "learned", the repetitive values are added up.

Procedure for creating a method

• Enter a command or select an existing command line.

• Press the <LEARN> key.

− Function is started, "LEARN" LED lights up.

− Press the <LEARN> key.

− Function is stopped, "LEARN" LED blinks.

− With the <ENTER> key, accept the value

(or re-start the LEARN function).

• "LEARN" LED goes out, edit next command line.

The LEARN function can be used with the following commands:

Command Teachable parameter Mode of function

LIFT Lift position in mm absolute PUMP Pump time in sec additive WAIT Waiting time in sec additive SCN Rm Status of the 8 remote lines "live" value SCN RS Character sequence received "live" value

4.3.5 TRACE function

The "TRACE" function is a valuable aid for operating through an entire

START

sequence or method (or parts thereof) for test purposes. Every command line in a sequence can be executed directly by pressing the <START> key. Upon completion of the action the next command line is displayed.

766 IC Sample Processor

Tracing can be executed immediately after entry of a sequence line or at any time after opening the parameter menu and selecting a sequence.

4 Operation

4.3.6 Commands for sequences

The following commands are programmable within a sequence. Most of them are also available in manual operation but may vary partially in their operation or exhibit a limited parameter selection (see section 4.4).

SAMPLE

>start sequence 1 SAMPLE: = 1

=,+,–; 1…999

Define current sample position

The SAMPLE command defines the rack position for the current sample (SAMPLE = X). This is stored as a index variable. It may be modified for example, in a sample sequence (SAMPLE + X or SAMPLE – X), in order to control the course of a sample series during processing.

The SAMPLE command does not have to be used for simple applications. As a general rule the first sample of a series is assumed to be in rack position 1 unless specified otherwise. Therefore it is recommended not to place the special beakers in the first rack positions; place them in the highest positions instead.

Before starting a sample series, the position of the first sample can be defined with the <SAMPLE> key in manual operation, as long as this has not been defined in the method itself.

If a certain sample tube order is needed for each application of a method, the position of the first sample can be defined in the start sequence with 'SAMPLE = X' and this setting can be saved with the corresponding method.

MOVE

If the SAMPLE command is not executed during a sample sequence, the SAMPLE variable will be increased by 1 after every processing of the sample sequence.

>sample sequence 2 MOVE 1 : sample

sample,spec.1…8,1…999

Position vessel / Turn rack

The MOVE command can place the current sample tube or a special beaker below the needle position by rotating the rack. An absolute rack position can also be specified.

During method processing a MOVE command automatically sets the lift to the shift position. In the parameter menu under '>changer settings' the turning speed can be defined specifically for each method.

If there is no sample tube in the rack position chosen, this is not recognized by the IC Sample Processor, and air is aspirated instead of sample solution. Therefore always make sure that sample tubes are placed at all rack positions defined in the processing sequence.

766 IC Sample Processor

LIFT

4.3 Methods

LIFT

PUMP

>sample sequence 3 LIFT: 1 : rest mm

work,rinse,shift,

special,rest,0…125 mm

Position the lift

Raising or lowering the lift to a defined position. Work, rinse, shift and special position are rack-specifically defined in the Configuration menu under '>rack definitions' (see section 4.2.1).

The rest position is the zero position (0 mm) of the lift, i.e. the upper stop.

Each lift can also be precisely positioned to the millimeter. The LEARN function is also available for this purpose (see section 4.3.4).

With LIFT commands, please note that if you are using the 6.1835.000 PEEK needle, the sample tubes may not be sealed with caps because they cannot be pierced by the PEEK needle !

>sample sequence 4 PUMP 1.1 : 1 s

Pump control

SCAN

1…999 s,ON,OFF

With the <PUMP> command the peristaltic pump of the 766 IC Sample Processor can be controlled.

The pump can be turned off and on as desired or operated for a specific amount of time. The LEARN function is useful for determining the interval of time (see section 4.3.4).

>sample sequence 5 SCN:Rm : Ready1

Rm,RS Ready1

End1

End2 Wait1 Wait2

Wait∗

Pump1 ? Pump2 ? Pump∗ ?

8 bit (1,0 or ∗)

Scanning the remote interface

= Waiting until 732/1 is ready = Waiting for EOD pulse of 732/1 = Waiting for EOD pulse of 732/2 = Waiting until remote line 3 of 732/1 is set to 1 = Waiting until remote line 3 of 732/2 is set to 1 = Waiting until remote line 3 of 732/1 and 732/2

are set to 1 = Waiting until IC Pump 709/1 is running = Waiting until IC Pump 709/2 is running = Waiting until IC Pumps 709/1 and 709/2 are

running

any 8-bit pattern

766 IC Sample Processor

In a sequence the SCN:Rm command causes method processing to stop until the predefined bit pattern is received.

4 Operation

Predefined bit patterns are supported which can be selected by short names (e.g. 'Ready1' or 'End2').

'Ready' signifies a static "ready" line of an external instrument. 'End'

stands for pulse signals, for example EOD (= end of determination). When scanning for pulse signals parallel scanning of several lines cannot be applied.

Setting special bit patterns allows flexible control of connected instruments.

Here the following is valid: 0 = line inactive

1 = line active

∗ = arbitrary line state

Example: 00000001 = input line 0 is active = instrument 1 "ready"

The bit pattern (= line state) can be taken over interactively with the LEARN function (see section 4.3.4).

For details about the remote interface, see section 5.1.

SCAN

>sample sequence 6 SCN:RS

Rm,RS

14 ASCII characters

Scanning the RS232 interface

arbitrary series of 14 characters

In a sequence the SCN:RS command causes method processing to stop until the predefined character string (up to 14 characters) is received via serial RS232 interface. The received data is compared character by character.

Be sure that the interface parameters agree with those of the instrument connected (see Configuration menu '>RS232 settings, section 4.2.1).

Any letters, numbers and special characters from the character set of the IC Sample Processor can be chosen. The asterisk '*' may be used as a wildcard for an arbitrary character or character string. (If '*' is to be interpreted as an ASCII character, '**' has to be set). A wildcard may be used in any position of a character string. If the first part of character string is correctly identified, the first appearance of the character following the asterisk '*' is scanned. When it is found, the next part of the character string is compared.

This function is especially suited to instruments with Metrohm remote control language. Here the autoinfo status messages can be scanned. For example, the 732 IC Detector has the following possibilities for scanning autoinfo information:

*R"* Ready, "Ready" state reached, e.g. at program end *S"* Stop, instrument manually stopped *W"* Instrument in wait status *E"* Error, error message

These status messages, however, are only transmitted if the corresponding status message has been previously switched on, for example, in the the start sequence (e.g. for the "Ready" message of the 732 IC Detector 732 with the command 'CTL:RS &Set.A.R"on"').

766 IC Sample Processor

CTL

4.3 Methods

More detailed information about the syntax can be found in the Instructions for Use of the instrument from which the status messages should be transmitted.

With the LEARN function transmitted data (= character strings) can be taken over interactively (see section 4.3.4).

CTRL

>sample sequence 7 CTL:Rm: INIT

Rm,RS INIT

INIT 732 PROG R/S 1 PROG R/S 2 PUMP R/S 1

FILL A 1 INJECT A 1

FILL B/STEP 1

INJECT B 1

ZERO 1

PUMP 752 ON PUMP 752 OFF STEP MSM 753

******0*001*** ******0*010*** ******1*000*** ******0*101***

******1*010*** ******0*011*** *******1******

*******0******

14 bit (1,0 or *)

Setting the remote lines

= initialize remote lines of 766 = initialize remote lines of 732/1 and 732/2 = run/stop time program at 732/1 = run/stop time program at 732/2 = run/stop IC Pump 709/1 = switch valve A at 733/1 to "Fill" = switch valve A at 733/1 to "Inject" = switch valve B at 733/1 to "Fill" or switch

suppressor to next position = switch valve B at 733/1 to "Inject" = trigger the autozero function at 732/1 = switch on pump at 752/753 = switch off pump at 752/753 = switch suppressor at 753 to next position = run/stop IC Pump 709/2 = switch valve A at 733/2 to "Fill" = switch valve A at 733/2 to "Inject" = switch valve B at 733/2 to "Fill" or switch

suppressor to next position = switch valve B at 733/2 to "Inject" = trigger the autozero function at 732/2 = switch on pump at 754 (with 6.2143.220 cable) = switch off pump at 754 (with 6.2143.220 cable)

arbitrary 14 bit pattern

CTRL

The CTL:Rm command controls external instruments via the remote interface. It causes the setting of defined line states or the sending of pulses via the 14 remote output lines.

Predefined bit patterns are supported which can be selected by short names (e.g. 'INIT 732' or 'ZERO 1').

Setting particular bit patterns allows flexible control of connected instruments.

Here the following is valid: 0 = line inactive

1 = line active

∗ = do not change line state

Example: ***000*******1 = Output line 0 active

= start/stop time program at 732/1

For details about the remote interface, see section 5.1.

>sample sequence 8 CTL:RS

Rm,RS

Clear value: &D.S"9"

14 ASCII characters

Data communication via

serial RS232 interface

= start/stop time program at 732 arbitrary string of 14 characters

766 IC Sample Processor

4 Operation

Data (= character string) can be sent to instruments connected via the serial RS232 interface.

Make sure that the transmission parameters of the RS232 interface correspond to those of the connected instrument (see Configuration menu '>RS232 settings', section 4.2.1).

Any letters, numbers and special characters can be chosen from the character set of the 766 IC Sample Processor.

This function is suitable for instruments with Metrohm remote control language. These can be controlled with so-called triggers.

&M;$G Go, start instrument in current mode &M;$S Stop, stop instrument &M;$Q Query, request of information

The following remote control commands (for example, in a start sequence) can switch on the autoinfo status messages of the 732 IC Detector 732:

&Set.A.R"on" "Ready" status message &Set.A.S"on" status message at manual stop &Set.A.W"on" status message at waiting state &Set.A.P"on" status message at switching on the instrument &Set.A.E"on" status message during an error condition

WAIT

ENDSEQ

To be consistent, the corresponding autoinfo messages should also be switched off again in a final sequence (..."off").

Detailed information about the syntax of the Metrohm remote control language can be found in section 5.2 or in the instruction manual of your instrument. Please keep the syntax and conventions of the foreign instruments or computers the 766 IC Sample Processor is communicating with.

>sample sequence 9 WAIT 1 s

0…1…9999 s

Waiting time

The WAIT command sets a particular waiting interval during method processing.

>sample sequence 10 ENDSEQ

End of the sequence

End mark of a sequence. This ENDSEQ command can be inserted in any desired command line for test purposes. This has the effect that the sequence (start, sample, or final sequence) is processed only up to this line.

766 IC Sample Processor

4.3.7 Process control

With <START> a method is started from the normal state. If there is no

START

STOP

manual intervention or unexpected errors, the sample series is correctly processed and closed with the final sequence. The sample sequence is executed repeatedly according to the entry under 'number of sam-

ples', beginning with the sample tube that is defined as 'SAMPLE'.

If the sample series is interrupted with <STOP>, the IC Sample Processor returns immediately to the normal state. Samples that have not been processed are ignored and the end sequence is not executed. If settings for such instances have been activated under 'manual stop

options', the corresponding actions or commands are also executed

via the interfaces to stop instruments that are connected or to initiate other actions.

4.3 Methods

LEARN

HOLD

RESET

CLEAR

QUIT

With <HOLD> the processing of a method can be interrupted. The command that is active at this point is immediately interrupted as a result. <START> continues the active sequence beginning with the command immediately following the one interrupted. Any peripheral instrument connected is not stopped with the <HOLD> key.

<CLEAR> interrupts a sample series at the end of a currently active sequence (soft break). The sample currently being processed will be completed.

<QUIT> interrupts the command currently being executed and starts the next command line in the sequence.

If errors occur during the sample series, the corresponding error mes- sage is displayed and must be acknowledged with <QUIT>. The IC Sample Processor then goes into the HOLD status (see above). After remedying the error, <START> resumes the sequence or <STOP> halts it entirely.

766 IC Sample Processor

During the course of a method it is possible to change all entries in the menus '>configuration' and '>parameters'. These changes have an immediate effect on the course of the method (with few exceptions, see

section 4.2.1).

Care should be taken when editing process sequences. These can be edited "live" (including insertion or deletion of a command line). The TRACE and LEARN functions however, are not available in this case. Testing of the edited function is therefore not possible. Illogical command sequences could easily result, which would cause an error condition and force the interruption of a sample series.

4 Operation

4.3.8 User defined methods

USER

METHOD

fined methods, which can be stored and recalled under freely selectable names. A method comprises the parameters defined under the <PARAM> key. The key opens the following main menu:

The <USER METHOD> key is used for the management of user de-

methods >recall method

methods >store method

methods >delete method

Repeated pressing of the <USER METHOD> key selects the submenus in turn. Use <ê> or <é> to move up or down one menu item; use <HOME> or <END> to move to the first or last menu item respectively. The submenu is opened with <ENTER>, <QUIT> returns to the normal state. The following listings show all dialog items which appear under <USER METHOD>. The values shown in the display are the default values, the possible entry values or ranges are shown below the display.

Recall method

Store method

Delete method

methods >recall method

>recall method method: ********

8 ASCII characters

methods >store methods

>store methods method: ********

8 ASCII characters

Recall existing method

Selection of the method

With <SELECT> any method saved can be chosen. If an "empty" method is to be loaded, the method

'********' can be selected. Like this, the actual work

memory is deleted.

Storage of methods

Define method name

"<" or ">" activates text edit mode where any method name desired can be entered (see section 4.1.6).

When a certain method should be worked off after switching on the IC Sample Processor, a command sequence can be stored under the name 'POWERUP'. This method is started automatically after switching on the main switch (see section 4.3.9).

766 IC Sample Processor

4.3 Methods

methods >delete method

>delete method method: ********

8 ASCII characters

>delete method delete ******** ?

Deletion of methods

4.3.9 POWERUP method

When the 766 IC Sample Processor is switched on, the sample rack and the needle are brought into the rest position. To bring them to other positions, the "POWERUP" method can be used. This method is started automatically, when the 766 IC Sample Processor is switched on.

Selection of the method

Selection of the method which should be deleted using the <SELECT> key.

Confirmation of the deletion

Confirmation with <ENTER> Abort with <QUIT>

Create a method that contains the command sequence which should be worked off, when the 766 IC Sample Processor is switched on. Store this method under the name 'POWERUP' (see section 4.3.8).

766 IC Sample Processor

4 Operation

4.4 Manual operation

4.4.1 Turning the sample rack / Positioning the samples

Using the <ç> and <è> keys the sample rack can be turned one position to the left (in the counterclockwise direction) or right (clockwise).

MOVE

4.4.2 Moving the lift

manual operation MOVE 1 : sample

sample,spec.1…8,

1…999

Position vessel / Turn rack

With the MOVE command, a particular sample tube or other vessel can be placed under the needle. With <SELECT> the numerical rack position as well as the predefined current sample (SAMPLE command) or the special beakers 1-8 can be chosen.

For security reasons turning the sample rack is only possible when the lift is in or above the shift position.

The keys <é> and <ê> allow upward and downward move- ment of the lift. The lowest possible lift position is defined by the configuration parameter 'max. lift way'.

HOME

LIFT

The <HOME> key runs the lift to the rest position (0 mm), i.e. to

END

the upper limit. <END> runs the lift to the predefined work position (see sec-

tion 4.3.2).

manual operation LIFT: 1 : rest mm

work,rinse,shift,

special,rest,

0…125 mm

Position the lift

With the LIFT command, the lift can be run to a given position. In addition to selecting an exact position in mm (0 - 125 mm), the <SELECT> key can select a predefined position (work position, rinse position, shift position, special position, rest position = 0 mm).

766 IC Sample Processor

4.4.3 Setting the sample position

4.4 Manual operation

SAMPLE

manual operation SAMPLE: = 1

The <SAMPLE> command serves to set the current sample position. It defines the first sample tube for the subsequent sample series.

44.4.4.4 .4 Pump control

PUMP

******** counter 1/128 PUMP+ ready

The PUMP command is used to switch on/off the peristaltic pump at the 766 IC Sample Processor. The pump command switches the selected pump on or off, according to the current state. The current state of the pumps is shown directly in the display:

Pump switched on: PUMP + (+ = ON)

Define current sample position

1…999

Switch pump on/off

PUMP+,PUMP-

Pump switched off: PUMP - (- = OFF) By pressing <STOP> the pump is also stopped.

4.4.5 Display interface signals

SCAN

manual operation SCN:Rm :00000000

Rm,RS 0,1

The SCN:Rm command causes the signal states of the incoming remote lines to be displayed in binary form (1 = line active, 0 = line inactive).

manual operation SCN:RS

Rm,RS ASCII

characters

The SCN:RS command causes the character string received at the RS232 interface to be displayed line by line (14 characters).

Display remote interface signals

Display RS232 interface signals

766 IC Sample Processor

4 Operation

4.4.6 Interface control

CTRL

manual operation CTL:Rm : INIT

Rm,RS INIT

INIT 732 PROG R/S 1 PROG R/S 2 PUMP R/S 1

FILL A 1 INJECT A 1

FILL B/STEP 1

INJECT B 1

ZERO 1

PUMP 752 ON PUMP 752 OFF STEP MSM 753

******0*001*** ******0*010*** ******1*000*** ******0*101***

******1*010*** ******0*011*** *******1******

*******0******

14 bit (1,0 or *)

Setting the remote lines

arbitrary 14 bit pattern

The CTL:Rm command controls external instruments via the remote interface. It causes the setting of defined line states or the sending of pulses via the 14 remote output lines. Predefined bit patterns are supported which can be selected by short names (e.g. 'INIT 732' or 'ZERO 1').

Here the following is valid: 0 = line inactive

1 = line active

∗ = do not change line state

Example: ***000*******1 = Output line 0 active

= start/stop time program at 732/1

For details about the remote interface, see section 5.1.

manual operation CTL:RS

Rm,RS

Clear value: &D.S"9"

14 ASCII characters

Data communication via RS232 interface

= start/stop time program at 732 arbitrary string of 14 characters

Using the CTL:RS command data (= character string) can be sent to instruments connected via the serial RS232 interface.

For details about the remote interface, see section 4.3.6 and section

5.2.

766 IC Sample Processor

44.4.4.7 .7 Print out reports

4.4 Manual operation

******** counter 1/128 Print: param

all

config

param

usermeth

Print out a report

All reports in the order usermeth, config, param

Configuration report Method parameter report Report of stored methods

The <PRINT> key is used for the output of reports on an external printer or PC connected to the RS232 interface (see section 2.5).

Example of "config" report

'co 766 IC Sample Proc. 0100 5.766.0010 configuration >auxiliaries dialog: english display contrast 3 beeper: ON device label ******** program 5.766.0010 max. lift way 125 mm >rack definitions number code type 1 000110 M128-2 work position 125 mm rinse position 125 mm shift position 0 mm special position 0 mm position special beaker 1...8 127 128 0 0 0 0 0 0 number code type 2 010001 M129-2 work position 125 mm rinse position 125 mm shift position 0 mm special position 0 mm position special beaker 1...8 128 129 0 0 0 0 0 0 number code type 3 001010 M142-2 work position 125 mm rinse position 125 mm shift position 0 mm special position 0 mm position special beaker 1...8 142 0 0 0 0 0 0 0 >RS232 settings baud rate: 9600 data bit: 8 stop bit: 1 parity: none handshake: HWs character set: IBM

------------

766 IC Sample Processor

4 Operation

Example of "param" report

'pa

766 IC Sample Proc. 0100 5.766.0010 parameters method ******** number of samples: rack >start sequence >sample sequence >final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

Example of "usermeth" report

'um 766 IC Sample Proc. 0100 5.766.0010 user memory >methods PC 760 PC Seg 808 SP 872 SP Seg 920 An Cat 1216 AnCatSeg 1304 Preconc 904 Dialysis 680 remaining bytes 26192

------------

766 IC Sample Processor

4.5 Sample racks

4.5.1 Standard rack (6.2041.430)

Metrohm delivers the following standard sample rack for the 766 IC Sample Processor:

4.5 Sample racks

Type Number of

M129-2 127

If desired, other user-defined racks can be delivered and defined in the instrument via PC software. Irregular arrangements of vessel positions are also possible.

4.5.2 Magnetic code

Every single rack can be identified by a unique magnetic code. Rod magnets which are attached to the bottom of the rack can be combined to form a 6-place binary code. The 766 IC Sample Processor can then automatically recognize the mounted rack. This is possible when the rack is positioned in the normal position. When changing a rack, the 766 IC Sample Processor should first be re-set to the normal position by pressing the <RESET> key or <ENDSEQ> and <ENTER>. This way the save recognition of a rack and therefore the correct vessel positioning is made possible. An internal position table containing the unambiguous definition of the turning angles and vessel positions is assigned to each rack type.

vessels

Type of vessel Predef.

code

PP Sample tube (6.2743.050; 11 mL) PE Bottle (6.1608.080; 300 mL)

010001 2

Predef.

rack no.

766 IC Sample Processor

When a sample series is started the IC Sample Processor first runs the rack automatically into the normal starting position so that it is always ensured that the vessel positions correspond with the internal position table of the current rack.

The rack code is used for automatic rack recognition. A code can be assigned only once. The standard rack supplied by Metrohm already have a predefined code. The rack code is a 6-place binary pattern with the digits 0 and 1 and this has to correspond to the order of the rod magnets. The digit 1 stands for a magnet which is inserted and 0 signifies that no magnet is inserted. 63 different codes are possible (000001 to 111111).

4 Operation

Magnets inserted

In this example the code is:

000110

Rack bottom view

In order to assign a certain sample rack for different applications, certain properties or recognition data can be defined for up to 16 racks. This is useful when a certain beaker size or the size of the sample series or a certain course of processing is to be predefined in an application.

4.5.3 Rack data

The following recognition data can be defined for each rack:

The Rack number serves to identify a rack. It can be chosen from 1 to

16. In a method a particular rack number can be assigned to the process sequences (see section 4.3.2). This ensures that if the wrong sample rack is used, the automatic rack recognition will realize this and issue a warning to the user.

Rack number unique identification Code automatic rack recognition Type rack type / position table Work position working height of the needle Rinse position rinsing height of the needle Shift position turning height of the needle Special position additional height of the needle Special beaker position reserved beaker positions

The Code is used for automatic rack recognition. Make sure that this 6place binary code in the rack configuration agrees with the actual inserted magnet code on the rack. Rack codes can be changed at any time. They must however, only be assigned to one rack. The assignment of standard predefined codes of standard racks provided by Metrohm should be avoided.

766 IC Sample Processor

4.5 Sample racks

The rack Type serves for the assignment to a position table internal to the instrument, in which the positions of the sample tubes in the rack are defined in tenths of a degree (0-3599) of the full turning angle. The rack type is coded as Mxxx-y, whereby M stands for Metrohm-defined types. The placeholder xxx stands for the number of sample tubes in a rack. The numerical code y is a special code for the number of rows on a rack (0 = single-row, 1 = double-row, 2 = triple-row). Position tables for user-defined rack types can be created with a suitable PC software and introduced into the instrument via the serial interface. The name of the rack type may be chosen at will this way.

The Work position determines the height of the needle in which the sample solution is aspirated. In this way the ideal position for every sample rack can be chosen dependent on the height of the sample tubes. This work position can be accessed directly in manual operation with the <END> key. In a process sequence this can be programmed with 'LIFT :1 : work mm'.

The Rinse position determines the correct position of the needle in which, for example, a rinsing solution should be aspirated. In this way the ideal position for every sample rack can be chosen dependent on the height of the sample tubes. This rinse position can be accessed with 'LIFT :1: rinse mm'.

The Shift position determines the correct position of the needle, in which the rack can be turned. This height must be 0 mm for the 766 IC Sample Processor 766. If the lift is not at or above the shift position, the sample rack can not be turned in manual operation. This is a safety feature to prevent damage to the needle due to turning maneuvers of the rack. However, a prerequisite is that this shift position is correctly set. In a process sequence the positioning of the lift to the shift position can be programmed with 'LIFT :1 : shift mm'.

The Special position determines an additional user defined height of the needle. This special position can be accessed with

'LIFT :1 : special mm'.

766 IC Sample Processor

4 Operation

4.5.4 Special beakers

Special beakers are reserved positions in a sample rack. 0 to 8 special beakers can be defined per rack. They can be placed under the needle during method processing for particular operations without interrupting or hindering the sample series run. Special beakers can be used in a sample sequence for rinsing the needle.

Special beakers are placed under the needle with 'MOVE 1 : spec.1'. Reserved special beaker positions, that can be individually defined for

each rack, are recognized as such in a sample series and are omitted during processing of the individual sample beakers.

For the 6.2041.430 sample rack, the two positions 128 and 129 are already defined as special positions for the 6.1608.080 PE bottles (300 mL).

Sample method for the use of special beakers

With this method 'PCRinse', modified from the standard method 'PC', the needle and the tubing leading to the sample loop is rinsed with rinsing solution during 60 s after each sample. For this method, a

6.1608.080 PE bottle filled with rinsing solution must be set as special beaker 2 on position 129 of the sample rack.

766 IC Sample Proc. 11111 5.766.0010 parameters method PCRinse number of samples: rack >start sequence 1 CTL:Rm: INIT >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump1 ? 3 MOVE 1 : sample 4 LIFT: 1 : work mm 5 CTL:Rm: FILL A 1 6 PUMP 1.1 : 120 s 7 CTL:Rm: ZERO 1 8 CTL:Rm: INJECT A 1 9 MOVE 1 : spec.2 10 LIFT: 1 : rinse mm 11 PUMP 1.1 : 60 s

>final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack

− Initialize remote interface

− Wait until 732 IC Det. sends signal on remote line 3

− Wait until 709 IC Pump runs

− Move needle to sample

− Place needle at working position

− Switch injection valve A at 733 to "Fill"

− Fill sample loop with sample during 120 s

− Trigger autozero at 732 IC Detector

− Switch injection valve A at 733 to "Inject"

− Move needle to rinsing solution

− Place needle at rinsing position

− Rinse tubing to injection valve during 60 s with rins-

ing solution

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

766 IC Sample Processor

4.6 Standard methods

The following pages contain the listing of standard user methods included with the instrument with explanations of the important commands. A prerequisite for the use of these methods is a correct configuration of the 766 IC Sample Processor for the 6.2041.430 sample rack supplied (M129-2).

The user methods are designed for IC systems with the Metrohm instruments 732 IC Detector, 733 IC Separation Center, 709 IC Pump, 752 Pump Unit, 753 Suppressor Module, and 754 Dialysis Unit and the PC evaluation program «IC Metrodata». The specific IC methods have to be programmed at the 732 IC Detector or via PC program «IC Metrodata». Details for the connection of these instruments to the remote connection 2222 can be found in section 2.4.

It is recommended to work through every new method step-by-step with the TRACE function, making adjustments where necessary, before starting the method for the first time.

4.6 Standard methods

4.6.1 Method "PC"

Application

Processing a queue with the PC program «IC Metrodata» with the PC as "Master".

Interconnection

see Fig. 11 (without suppression) and Fig. 13 (with suppression)

Program of 766 IC Sample Processor

766 IC Sample Proc. 11111 5.766.0010 parameters method PC number of samples: rack >start sequence 1 CTL:Rm: INIT >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump1 ? 3 MOVE 1 : sample 4 LIFT: 1 : work mm 5 CTL:Rm: FILL A 1 6 PUMP 1.1 : 120 s 7 CTL:Rm: ZERO 1 8 CTL:Rm: INJECT A 1 >final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack)

− Initialize remote interface

− Waiting until 732 IC Det. sends signal on rem. line 3

− Waiting until 709 IC Pump runs

− Move needle to sample

− Place lift with needle to working position

− Switch injection valve A at 733 to "Fill"

− Fill sample loop with sample during 120 s

− Trigger autozero at 732 IC Detector

− Switch injection valve A at 733 to "Inject"

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

766 IC Sample Processor

4 Operation

Settings in the «IC Metrodata» program

Definition of IC method (analysis time, start parameters for IC instruments 732, 733 and 709, etc.), additional association of this method with the following time program for the 732 IC Detector:

Method / Equipment / Setup / Program / Text:

0.0 Puls_766_set Set remote line 3 at 732/1 to 1 (s. below)

0.1 Puls_766_reset Set remote line 3 at 732/1 to 0 (s. below)

0.2 Flag end Program end

Method / Equipment / Setup / Program / Remote configuration:

Puls_766_set = **1***** Set remote line 3 at 732/1 to 1 Puls_766_reset = **0***** Set remote line 3 at 732/1 to 0

Method / Equipment:

Start with method

Procedure at program start

1. Start 766 IC Sample Processor with <START>.

2. Create new queue in the program «IC Metrodata» and start it.

4.6.2 Method "PC Seg"

Application

Processing a queue with the PC program «IC Metrodata» with the PC as "Master", additional insertion of a defined air bubble between the samples.

Interconnection

see Fig. 11 (without suppression) and Fig. 13 (with suppression)

Program of 766 IC Sample Processor

766 IC Sample Proc. 11111 5.766.0010 parameters method PC Seg number of samples: rack >start sequence 1 CTL:Rm: INIT >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump1 ? 3 MOVE 1 : sample 4 PUMP 1.1 : 5 s 5 LIFT: 1 : work mm 6 CTL:Rm: FILL A 1 7 PUMP 1.1 : 120 s 8 CTL:Rm: ZERO 1 9 CTL:Rm: INJECT A 1 >final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack)

− Initialize remote interface

− Waiting until 732 IC Det. sends signal on rem. line 3

− Waiting until 709 IC Pump runs

− Move needle to sample

− Aspirate air into the transfer tubing during 5 s

− Place lift with needle to working position

− Switch injection valve A at 733 to "Fill"

− Fill sample loop with sample during 120 s

− Trigger autozero at 732 IC Detector

− Switch injection valve A at 733 to "Inject"

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

766 IC Sample Processor

4.6 Standard methods

Settings in the «IC Metrodata» program

Definition of IC method (analysis time, start parameters for IC instruments 732, 733 and 709, etc.), additional association of this method with the following time program for the 732 IC Detector:

Method / Equipment / Setup / Program / Text:

0.0 Puls_766_set Set remote line 3 at 732/1 to 1 (s. below)

0.1 Puls_766_reset Set remote line 3 at 732/1 to 0 (s. below)

0.2 Flag end Program end

Method / Equipment / Setup / Program / Remote configuration:

Puls_766_set = **1***** Set remote line 3 at 732/1 to 1 Puls_766_reset = **0***** Set remote line 3 at 732/1 to 0

Method / Equipment:

Start with method

Procedure at program start

1. Start 766 IC Sample Processor with <START>.

2. Create new queue in the program «IC Metrodata» and start it.

4.6.3 Method "SP"

Application

Processing a queue with the PC program «IC Metrodata» with the 766 IC Sample Processor as "Master".

Interconnection

see Fig. 11 (without suppression) and Fig. 12 (with suppression)

Program of 766 IC Sample Processor

766 IC Sample Proc. 11111 5.766.0010 parameters method SP number of samples: rack >start sequence 1 CTL:Rm: INIT 2 CTL:Rm: PUMP 752 ON >sample sequence 1 SCN:Rm : Pump1 ? 2 MOVE 1 : sample 3 LIFT: 1 : work mm 4 CTL:Rm: FILL A 1 5 PUMP 1.1 : 120 s 6 CTL:Rm: ZERO 1 7 CTL:Rm: INJECT A 1 8 WAIT 1200 s >final sequence 1 CTL:Rm: PUMP R/S 1 2 CTL:Rm: PUMP 752 OFF >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack)

− Initialize remote interface

− Switch on 752 Pump Unit for suppressor

− Waiting until 709 IC Pump runs

− Move needle to sample

− Place lift with needle to working position

− Switch injection valve A at 733 to "Fill"

− Fill sample loop with sample during 120 s

− Trigger autozero at 732 IC Detector

− Switch injection valve A at 733 to "Inject"

− Waiting time for recording and evaluation of the

chromatogram (must be adapted)

− Switch off 709 IC Pump

− Switch off 752 Pump Unit

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

766 IC Sample Processor

4 Operation

Settings for 732 IC Detector

Setting the measurement parameters.

Settings in the «IC Metrodata» program

Definition of an IC method without method associated remote control. The analysis time Duration must be equal to the waiting time WAIT entered under sample sequence in the 766 IC Sample Processor.

Procedure at program start

1. Create new queue in the program «IC Metrodata» and start it.

2. Enter parameter number of samples for 766 IC Sample Processor.

3. Start 766 IC Sample Processor with <START>.

4.6.4 Method "SP Seg"

Application

Processing a queue with the PC program «IC Metrodata» with the 766 IC Sample Processor as "Master", additional insertion of a defined air bubble between the samples.

Interconnection

see Fig. 11 (without suppression) and Fig. 12 (with suppression)

Program of 766 IC Sample Processor

766 IC Sample Proc. 11111 5.766.0010 parameters method SP Seg number of samples: rack >start sequence 1 CTL:Rm: INIT 2 CTL:Rm: PUMP 752 ON >sample sequence 1 SCN:Rm : Pump1 ? 2 MOVE 1 : sample 3 PUMP 1.1 : 5 s 4 LIFT: 1 : work mm 5 CTL:Rm: FILL A 1 6 PUMP 1.1 : 120 s 7 CTL:Rm: ZERO 1 8 CTL:Rm: INJECT A 1 9 WAIT 1200 s >final sequence 1 CTL:Rm: PUMP R/S 1 2 CTL:Rm: PUMP 752 OFF >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack)

− Initialize remote interface

− Switch on 752 Pump Unit for suppressor

− Waiting until 709 IC Pump runs

− Move needle to sample

− Aspirate air into the transfer tubing during 5 s

− Place lift with needle to working position

− Switch injection valve A at 733 to "Fill"

− Fill sample loop with sample during 120 s

− Trigger autozero at 732 IC Detector

− Switch injection valve A at 733 to "Inject"

− Waiting time for recording and evaluation of the

chromatogram (must be adapted)

− Switch off 709 IC Pump

− Switch off 752 Pump Unit

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

766 IC Sample Processor

Settings for 732 IC Detector

Setting the measurement parameters.

Settings in the «IC Metrodata» program

Definition of an IC method without method associated remote control. The analysis time Duration must be equal to the waiting time WAIT entered under sample sequence in the 766 IC Sample Processor.

Procedure at program start

1. Create new queue in the program «IC Metrodata» and start it.

2. Enter parameter number of samples for 766 IC Sample Processor.

3. Start 766 IC Sample Processor with <START>.

4.6.5 Method "An Cat"

Application

Simultaneous determination of anions and cations.

Interconnection

see Fig. 14

4.6 Standard methods

Program of 766 IC Sample Processor

766 IC Sample Proc. 11111 5.766.0010 parameters method An Cat number of samples: rack >start sequence 1 CTL:Rm: INIT >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump* ? 3 MOVE 1 : sample 4 LIFT: 1 : work mm 5 CTL:Rm: FILL A 1 6 CTL:Rm: STEP MSM 753 7 PUMP 1.1 : 150 s 8 CTL:Rm: ZERO 1 9 CTL:Rm: INJECT A 1 10 SAMPLE: + 1 11 MOVE 1 : sample 12 LIFT: 1 : work mm 13 CTL:Rm: ******0*010*** 14 CTL:Rm: INIT 732 15 PUMP 1.1 : 150 s 16 CTL:Rm: ******0*011*** 17 CTL:Rm: INIT 732 18 CTL:Rm: ******1*000*** 19 CTL:Rm: INIT 732 20 SAMPLE: + 1 >final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack)

− Initialize remote interface

− Waiting until 732 IC Det. sends signal on rem. line 3

− Waiting until the two 709 IC Pumps run

− Move needle to sample position

− Place lift with needle to working position

− Switch injection valve A at 733 to "Fill"

− Switch 753 suppressor module to next position

− Fill sample loop A with sample during 150 s

− Trigger autozero at 732/1 IC Detector

− Switch injection valve A at 733 to "Inject"

− Raise sample position by 1

− Move needle to sample position

− Place lift with needle to working position

− Switch injection valve B at 733 to "Fill"

− Initialize remote lines at 732/1 and 732/2

− Fill sample loop B with sample during 150 s

− Trigger autozero at 732/2 IC Detector

− Initialize remote lines at 732/1 and 732/2

− Switch injection valve B at 733 to "Inject"

− Initialize remote lines at 732/1 and 732/2

− Raise sample position by 1

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

766 IC Sample Processor

4 Operation

Settings in the «IC Metrodata» program

1. Definition of anion method for channel 1 (analysis time, start parameters for IC instruments 732, 733 and 709, etc.), additional association of this method with the following time program for the 732/1 IC Detector:

Method / Equipment / Setup / Program / Text:

0.0 Puls_766_set Set remote line 3 at 732/1 to 1 (s. below)

0.1 Puls_766_reset Set remote line 3 at 732/1 to 0 (s. below)

0.2 Flag end Program end

Method / Equipment / Setup / Program / Remote configuration:

Puls_766_set = **1***** Set remote line 3 at 732/1 to 1 Puls_766_reset = **0***** Set remote line 3 at 732/1 to 0

Method / Equipment:

Start with method

2. Definition of cation method for channel 2 (analysis time, start parameters for IC instruments 732, 733 and 709, etc.). The analysis time Duration must be shorter than the analysis time for the anion method.

Procedure at program start

1. Create queue with cation method for channel 2 in the program «IC Metrodata» and start it.

2. Start 766 IC Sample Processor with <START>.

3. Create queue with anion method for channel 1 in the program «IC Metrodata» and start it.

4.6.6 Method "AnCatSeg"

Application

Simultaneous determination of anions and cations, additional insertion of a defined air bubble between the samples.

Interconnection

see Fig. 14

Program of 766 IC Sample Processor

766 IC Sample Proc. 11111 5.766.0010 parameters method AnCatSeg number of samples: rack >start sequence 1 CTL:Rm: INIT >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump* ? 3 MOVE 1 : sample 4 PUMP 1.1 : 5 s 5 LIFT: 1 : work mm 6 CTL:Rm: FILL A 1

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack)

− Initialize remote interface

− Waiting until 732 IC Det. sends signal on rem. line 3

− Waiting until the two 709 IC Pumps run

− Move needle to sample position

− Aspirate air into the transfer tubing during 5 s

− Place lift with needle to working position

− Switch injection valve A at 733 to "Fill"

766 IC Sample Processor

4.6 Standard methods

7 CTL:Rm: STEP MSM 753 8 PUMP 1.1 : 150 s 9 CTL:Rm: ZERO 1 10 CTL:Rm: INJECT A 1 11 SAMPLE: + 1 12 MOVE 1 : sample 13 PUMP 1.1 : 5 s 14 LIFT: 1 : work mm 15 CTL:Rm: ******0*010*** 16 CTL:Rm: INIT 732 17 PUMP 1.1 : 150 s 18 CTL:Rm: ******0*011*** 19 CTL:Rm: INIT 732 20 CTL:Rm: ******1*000*** 21 CTL:Rm: INIT 732 22 SAMPLE: + 1 >final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

− Switch 753 suppressor module to next position

− Fill sample loop A with sample during 150 s

− Trigger autozero at 732/1 IC Detector

− Switch injection valve A at 733 to "Inject"

− Raise sample position by 1

− Move needle to sample position

− Aspirate air into the transfer tubing during 5 s

− Place lift with needle to working position

− Switch injection valve B at 733 to "Fill"

− Initialize remote lines at 732/1 and 732/2

− Fill sample loop B with sample during 150 s

− Trigger autozero at 732/2 IC Detector

− Initialize remote lines at 732/1 and 732/2

− Switch injection valve B at 733 to "Inject"

− Initialize remote lines at 732/1 and 732/2

− Raise sample position by 1

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

Settings in the «IC Metrodata» program

Method / Equipment / Setup / Program / Text:

0.0 Puls_766_set Set remote line 3 at 732/1 to 1 (s. below)

0.1 Puls_766_reset Set remote line 3 at 732/1 to 0 (s. below)

0.2 Flag end Program end

Method / Equipment / Setup / Program / Remote configuration:

Puls_766_set = **1***** Set remote line 3 at 732/1 to 1 Puls_766_reset = **0***** Set remote line 3 at 732/1 to 0

Method / Equipment:

Start with method

Procedure at program start

1. Create queue with cation method for channel 2 in the program «IC Metrodata» and start it.

2. Start 766 IC Sample Processor with <START>.

766 IC Sample Processor

3. Create queue with anion method for channel 1 in the program «IC Metrodata» and start it.

4 Operation

Waste

Sample

Waste

Sample injection

Preconcentration

IC column

Preconc. column

Eluent

IC column

Eluent

4.6.7 Method "Preconc"

Application

Preconcentration of samples with very low concentrations in an preconcentration column (trace analysis).

Interconnection

see Fig. 13

Installation of the preconcentration column

The preconcentration column, consisting of the 6.1006.200 Metrosep Anion preconcentration cartridge and the 6.2828.010 glass cartridge holder, is installed at the injection valve of the 733 IC Separation Center instead of the sample loop (see Fig. 18). It is important to install the sample inlet from the 766 IC Sample Processor at connection 2 of the injection valve in order to run the preconcentration column for the enrichment and the sample injection in the opposite direction. If a larger sample throughput is desired, install the optionally available 6.1826.040 pump tubing (inner diameter 1.6 mm, flow ca. 3 mL/min) at the peristaltic pump of the 766 IC Sample Processor.

3 6

Fig. 18: Installation of the preconcentration column

Program of 766 IC Sample Processor

766 IC Sample Proc. 11111 5.766.0010 parameters method Preconc number of samples: rack >start sequence 1 CTL:Rm: INIT 2 CTL:Rm: INJECT A 1 >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump1 ? 3 MOVE 1 : sample 4 LIFT: 1 : work mm 5 PUMP 1.1 : ON s 6 WAIT 60 s 7 CTL:Rm: FILL A 1 8 WAIT 120 s

9 PUMP 1.1 : OFF s 10 CTL:Rm: ZERO 1 11 CTL:Rm: INJECT A 1

4 5

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack)

− Initialize remote interface

− Switch injection valve A at 733 to "Inject"

− Waiting until 732 IC Det. sends signal on rem. line 3

− Waiting until 709 IC Pump runs

− Move needle to sample position

− Place lift with needle to working position

− Switch on pump at 766

− Waiting time 60 s

− Switch injection valve A at 733 to "Fill"

− Sample preconcentration during 120 s

(preconcentration time must be adapted)

− Switch off pump at 766

− Trigger autozero at 732 IC Detector

− Switch injection valve A at 733 to "Inject"

3 6

4 5

766 IC Sample Processor

4.6 Standard methods

>final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

Settings in the «IC Metrodata» program

Definition of IC method (analysis time, start parameters for IC instruments 732, 733 and 709, etc.), additional association of this method with the following time program for the 732 IC Detector:

Method / Equipment / Setup / Program / Text:

0.0 Puls_766_set Set remote line 3 at 732/1 to 1 (s. below)

0.1 Puls_766_reset Set remote line 3 at 732/1 to 0 (s. below)

0.2 Flag end Program end

Method / Equipment / Setup / Program / Remote configuration:

Puls_766_set = **1***** Set remote line 3 at 732/1 to 1 Puls_766_reset = **0***** Set remote line 3 at 732/1 to 0

Method / Equipment:

Start with method

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

Procedure at program start

1. Start 766 IC Sample Processor with <START>.

2. Create new queue in the program «IC Metrodata» and start it.

4.6.8 Method "Dialysis"

Application

IC determinations with automatic sample dialysis.

Interconnection

see Fig. 13 and section 2.3.9 as well as 754 Instructions for Use

Program of 766 IC Sample Processor

766 IC Sample Proc. 11111 5.766.0010 parameters method Dialysis number of sample: rack >start sequence 1 CTL:Rm: INIT >sample sequence 1 SCN:Rm : Pump1 ? 2 MOVE 1 : sample 3 LIFT: 1 : work mm 4 CTL:Rm: PROG R/S 1 5 SCN:Rm : Ready1

− Report header with serial number and program ver-

sion

− Method name

− Number of samples to be processed (entire sample

rack)

− Initialize remote interface

− Waiting until 709 IC Pump runs

− Move needle to sample position

− Place lift with needle to working position

− Start time program at 732

− Waiting until 732 IC Detector sends ready signal

766 IC Sample Processor

4 Operation

>final sequence 1 CTL:Rm: PUMP R/S 1 >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232:

------------

Settings for 732 IC Detector

Setting of measurement parameters and creation of a program according to section 3.3.2 of the 754 Instruction for Use. For the program type, select remote instead of cycle.

Settings in the «IC Metrodata» program

Definition of an IC method without method associated remote control. The analysis time Duration must be ca 1 min shorter than the analysis time entered in the 732 IC Detector.

Procedure at program start

1. Enter parameter number of samples for 766 IC Sample Processor.

2. Create new queue in the program «IC Metrodata» and start it.

3. Start 766 IC Sample Processor with <START>.

− Switch off 709 IC Pump

− —— Settings for changer functions ——

− —— Reaction to manual stop ———

766 IC Sample Processor

5 Interfaces

5.1 Remote interface

Peripheral instruments connected such as 732 IC Detector, 709 IC Pump, 752 Pump Unit, etc. can be controlled via the remote interface (25-pin socket).

14 lines (Output 0 – 13) are available for the emission signals. Lines 11 – 13 are used for swing head control.

5.1 Remote interface

For receiving signals (e.g. the "ready" signal of the 732 IC Detector) 8 lines (Input 0 – 7) are provided. Line 7 is used for the swing head.

5.1.1 Pin assignment of the remote socket:

14 1

1325

>20 ms

Output 6 Output 7 Output 4 Output 2 Output 0 Output 8 Output 9 Output 10 Input 1 Input 3 Input 5 Input 7 Output 11

0 Volt

+5 Volt Output 5 Output 3 Output 1

Output 12 Output 13

Input 0

Input 2

Input 4

Input 6

0 Volt

Inputs

+5V

active = low inactive = high

766 IC Sample Processor

Outputs

>200 ms

CEO

= 40 V = 20 mA

active = low inactive = high

+5V I ≤ 20 mA

The +5 V supply line may by charged with 20 mA maximally.

5 Interfaces

For the 766 IC Sample Processor, the output lines 11–13 and the input line 7 are occupied for the control of the swing head. These four lines are not continued in the plug and ignored, when further instruments are connected via the remote cable (see section 2.4).

Various remote cables are available to use the specific functions of the individual instruments of the various Metrohm model lines (see section

2.4). Metrohm also delivers special cables on request suited to the customer’s needs, which allow complex couplings (including foreign instruments).

5.1.2 Functional characteristics

Output lines

The 14 output lines of the remote socket can be separately set (statically) in manual operation as well as during method processing with the "Control" command (CTL). A 14-place bit pattern must be defined for this. Every bit is assigned to an output line.

Output 13 12 11 10 9 8 7 6 5 4 3 2 1 0 Bit 13 12 11 10 9 8 7 6 5 4 3 2 1 0

(Bits are always numbered from right to left)

Example: CTL Rm ************1*

sets the output line 1 to active (=set), that for example, would switch on the pump of a connected 752 Pump Unit.

0 = inactive (high) 1 = active (low) * = no change

It is recommended to mask the output lines that are not relevant with a asterisk (*) to prevent alterations to these line states.

766 IC Sample Processor

5.1 Remote interface

Input lines

The 8 input lines of the remote socket are queried during method processing with the "Scan" command (SCN). Method processing is interrupted until the predefined bit pattern compares to the effective state of the input lines (for example, the status of the ready line of the 732 IC Detector). An 8-place bit pattern must be set for this. Every bit is assigned to an input line. If there is correspondence, method processing will continue with the next command line. During manual operation the SCAN command serves as a status display of all input lines.

Input 7 6 5 4 3 2 1 0 Bit 7 6 5 4 3 2 1 0

(Bits are always numbered from right to left)

Example: SCN Rm *******1

expects an active input line 0 (1=set or active). This line is set for example, by the 732 IC Detector, if no program runs.

0 = inactive (high) 1 = active (low) * = arbitrary

Input lines that are not being used or for which no defined state can be predicted, should also be masked here with an asterisk (*).

Control of instruments

With a suitable multi-cable (with special wiring) or with the 6.2125.120 adaptor several instruments can be controlled simultaneously via the remote lines (see section 2.4). The bit patterns for the CTL and SCN commands can be combined for this. Be aware, however, that some instruments only transmit short impulses (typically 20 ms) at the end of a determination and therefore a combined query of the end of determination with other instruments is only possible under certain conditions (dependent upon time).

To simplify the use of these remote control commands especially when connecting several instruments with Metrohm cables, the following command parameters are available for the CTL and SCN commands. Beside these command parameters the first table contains some further useful, but not implemented bit patterns for the control of IC instruments, which are sent as static signals and not as pulses as for the predefined commands. In order to get such commands becoming effective, the command 'INIT 732' to reset the remote lines at 732/1 and 732/2 must be sent after each command.

766 IC Sample Processor

5 Interfaces

CTL commands

Parameter Bit pattern Function Signal

INIT 00000000000000 initializes the remote interface static INIT 732 ***0000*000**0 initializes the remote lines at 732/1 and 732/2 static PROG R/S 1 ***000*******1 starts/stops time program at 732/1 pulse (200 ms) PROG R/S 2 ******0*100*** starts/stops time program at 732/2 pulse (200 ms) PUMP R/S 1 ***001*******0 starts/stops 709/1 IC Pump pulse (200 ms) FILL A 1 ***010*******0 switches valve A at 733/1 to "Fill" pulse (200 ms) INJECT A 1 ***100*******0 switches valve A at 733/1 to "Inject" pulse (200 ms) FILL B/STEP 1 ***001*******1 switches valve B at 733/1 to "Fill" pulse (200 ms) INJECT B 1 ***110*******0 switches valve B at 733/1 to "Inject" pulse (200 ms) ZERO 1 ***011*******0 triggers autozero at 732/1 pulse (200 ms) PUMP 752 ON ************1* switches on pump at 752/753 static PUMP 752 OFF ************0* switches off pump at 752/753 static STEP MSM 753 ***********1** switches 753 suppressor module to next position pulse (200 ms)

[PUMP R/S 2 ] ******0*001*** starts/stops 709/2 IC Pump static [FILL A 2 ] ******0*010*** switches valve A at 733/2 to "Fill" static [INJECT A 2 ] ******1*000*** switches valve A at 733/2 to "Inject" static [FILL B/STEP 2] ******0*101*** switches valve B at 733/2 to "Fill" static [INJECT B 2 ] ******1*010*** switches valve B at 733/2 to "Inject" static [ZERO 2 ] ******0*011*** triggers autozero at 732/2 static [PUMP 754 ON ] *******1****** switches on pump at 754 (with 6.2143.220 cable) static [PUMP 754 OFF ] *******0****** switches off pump at 754 (with 6.2143.220 cable) static

SCAN commands

Parameter Bit pattern Function

Ready1 *******1 waits until the "ready" state of 732/1 is reached End1 ****1*** waits until the 732/1 sends the EOD signal End2 *1****** waits until the 732/2 sends the EOD signal Wait1 *****1** waits until the 732/1 sets the remote line 3 to 1 Wait2 ***1**** waits until the 732/2 sets the remote line 3 to 1 Wait* ***1*1** waits until the 732/1 and 732/2 set their remote line 3 to 1 Pump1 ? ******1* waits until the 709/1 IC Pump is running Pump2 ? **1***** waits until the 709/2 IC Pump is running Pump* ? **1***1* waits until the 709/1 and 709/2 IC Pumps are running

766 IC Sample Processor

5.2 RS232 interface

5.2.1 General rules for remote control

The 766 IC Sample Processor is equipped with the comprehensive Metrohm remote control language, which allows full control over the instrument via an RS232 interface, i.e. the 766 IC Sample Processor can receive data from an external device or send data to an external device. The 766 IC Sample Processor sends 2×CRR and L requested data block. In contrast, CRR and LFF are used as the terminator of a data line. On receipt of data from an external device, this must always close its commands with CRR and LFF. If more than one command is sent on a line, ‘;’ must be used as a delimiter between the individual commands.

The data are grouped logically and readily understandable. For example, to select the dialog language the command

&Config.Aux.Language"english"

must be sent with entry of the boldface characters sufficing, in other words

&C.A.L"english"

5.2 RS232 interface

as the terminator of a

F F

All quantities of the 766 IC Sample Processor are collected in groups. The entries for the configuration, for example, are located in the group

&Config

The ‘Config’ group contains sub-groups, e.g. for setting the RS232 interface parameters

&Config.RSset

or for various settings

&Config.Aux

The data have a hierarchical structure (tree structure). The quantities which appear in this tree are called objects in what follows. The dialog language is that object which is called up with the command

&Config.Aux.Language

Once you are at the desired location in the tree, you can request the value of the object:

&Config.Aux.Language $Q Q for query

The inquiry ‘$Q’ initiates the output of the value on the instrument, in other words the value output is triggered. Entries which begin with the character ‘$’ always trigger something and are hence referred to as triggers in what follows.

766 IC Sample Processor

However, values of objects can not only be requested, they can also be modified. Values are always inputted in inverted commas, e.g.

&Config.Aux.Language"english"

5 Interfaces

5.2.2 Call-up of objects

A section from the object tree is shown below:

Node 3

Node 2

Node 1

Language Prog

Aux

RSset

Config

Mode

Node 0

Root

The following rules apply to the call-up of objects:

Rules Examples

The root of the tree is designated by ‘&’.

For the call-up of an object, the nodes (levels) of the tree are marked by a point (.).

For the call-up of the objects, as many characters as necessary to allow unambiguous allocation of the object suffice. If the call-up is not unambiguous, the first object in the series is identified.

Call up of the dialog language:

&Config.Aux.Language

&C.A.L

Uppercase and lowercase letters can be used. &C.A.L or &c.a.l

A value can be assigned to an object. Values are marked at both their beginning and end by inverted commas ("). They can contain maximum 24 ASCII characters. In the case of parameters with specified text expressions (e.g. on, off), only the English expressions may be used. Numeric values can contain up to 6 digits, a negative sign and a decimal point. Numbers with more than 6 digits are not accepted; more than 4 decimal places are rounded off. With numbers <1, leading zeros must be entered.

Until a new object is called up, the old object remains in force.

New objects can be addressed relative to the old object:

A leading point leads one node forwards in the tree.

More than one leading point leads one node backwards

in the tree. n nodes backwards require n+1 leading points.

To return to the root, enter a leading '&'. Switch from node 'Language' via

Entry of the dialog language:

&C.A.L"english"

Correct numeric entries:

"0.1"

Incorrect numeric entries: "1,5" or "+3" or ".1"

Entry of a different dialog language: "deutsch"

From the root to the node 'Aux':

&C.A

Forwards from the node 'Aux' to 'Prog': .P

Jump from node 'Prog' to the node 'Aux' and selection of the new object 'Language' at this node: ..L

the root to node 'Mode': &M

766 IC Sample Processor

5.2.3 Trigger

5.2 RS232 interface

Triggers initiate an action at the 766 IC Sample Processor, e.g. sequence start or data transmission. Triggers are marked by the introducer '$'.

The following triggers are possible:

$G Go Starts processes, e.g. starting the mode run or set-

ting the RS232 interface parameters

$S Stop Stops processes

$Q Query Used to request all information from the current

node in the tree forwards up to and including the values

$Q.P Path Used to request the path from the root of the tree

up to the current node

$Q.H Highest

Index

$Q.N"i" Name Used to request the name of the daughter node

$D Detail-Info Used to request detailed status information

$U qUit Used to terminate the data flow of the instrument,

Used to request the number of daughter nodes of the current node

with index i, i = 1...n

e.g. after $Q

The triggers '$G' and '$S' are linked to particular objects, see Overview table in section 5.2.6.

All other triggers can always be used at all locations in the data tree. Examples:

Request of the baud rate value: &Config.RSset.Baud $Q Request of all values of the node 'RSset': &Config.RSset $Q Request of the path of the node 'RSset': &Config.RSset $Q.P Start mode: &Mode $G Request of the detailed status: $D

766 IC Sample Processor

Metrohm 766IC User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual